negative regulation of Rho guanyl-nucleotide exchange factor activity
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| TIR | PF01582 | 17 - 192 | 2.9e-55 | TIR domain |
| TIR_2 | PF13676 | 20 - 110 | 3.6e-06 | TIR domain |
| NB-ARC | PF00931 | 208 - 362 | 6.4e-15 | NB-ARC domain |
| WHD_ROQ1 | PF23282 | 430 - 498 | 1.2e-12 | Disease resistance protein Roq1-like, winged-helix domain |
| LRR_3 | PF07725 | 602 - 621 | 2.6e-09 | Leucine Rich Repeat |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1625 |
| Acc16I | TGCGCA | 1 cut(s) | 110 |
| AccI | GTMKAC | 2 cut(s) | 700, 1480 |
| AccII | CGCG | 1 cut(s) | 1735 |
| AccIII | TCCGGA | 1 cut(s) | 1921 |
| AciI | CCGC | 3 cut(s) | 1629, 1733, 2594 |
| AclWI | GGATC | 7 cut(s) | 140, 221, 1447, 1932, 1965, 1981, 2843 |
| AcsI | RAATTY | 5 cut(s) | 368, 1888, 2597, 2882, 2894 |
| AcuI | CTGAAG | 1 cut(s) | 2877 |
| AcvI | CACGTG | 1 cut(s) | 1024 |
| AdeI | CACNNNGTG | 1 cut(s) | 313 |
| AfaI | GTAC | 9 cut(s) | 180, 351, 876, 1019, 1260, 1570, 2541, 2702, 2778 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 1150, 2645, 2680 |
| AflII | CTTAAG | 1 cut(s) | 475 |
| AflIII | ACRYGT | 1 cut(s) | 392 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 2634 |
| AjnI | CCWGG | 1 cut(s) | 2638 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 359, 391, 2066, 2098 |
| Alw21I | GWGCWC | 4 cut(s) | 400, 1710, 1818, 2490 |
| Alw26I | GTCTC | 8 cut(s) | 9, 1715, 1905, 2222, 2231, 2374, 2444, 3091 |
| Alw44I | GTGCAC | 2 cut(s) | 396, 1706 |
| AlwI | GGATC | 7 cut(s) | 140, 221, 1447, 1932, 1965, 1981, 2843 |
| Ama87I | CYCGRG | 1 cut(s) | 61 |
| Aor13HI | TCCGGA | 1 cut(s) | 1921 |
| AoxI | GGCC | 2 cut(s) | 74, 2515 |
| ApaLI | GTGCAC | 2 cut(s) | 396, 1706 |
| ApeKI | GCWGC | 3 cut(s) | 994, 2238, 2373 |
| ApoI | RAATTY | 5 cut(s) | 368, 1888, 2597, 2882, 2894 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 753, 792, 2319 |
| AspLEI | GCGC | 2 cut(s) | 111, 1866 |
| AspS9I | GGNCC | 2 cut(s) | 74, 653 |
| AsuC2I | CCSGG | 2 cut(s) | 364, 2577 |
| AsuHPI | GGTGA | 5 cut(s) | 1355, 2488, 2597, 2819, 3020 |
| AsuII | TTCGAA | 1 cut(s) | 2880 |
| AvaI | CYCGRG | 1 cut(s) | 61 |
| AvaII | GGWCC | 1 cut(s) | 653 |
| AxyI | CCTNAGG | 1 cut(s) | 1716 |
| BaeGI | GKGCMC | 2 cut(s) | 400, 1710 |
| BanII | GRGCYC | 2 cut(s) | 1818, 2490 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 162, 194, 1197, 1229 |
| BbrPI | CACGTG | 1 cut(s) | 1024 |
| BbsI | GAAGAC | 6 cut(s) | 49, 474, 1148, 1315, 1772, 2997 |
| Bbv12I | GWGCWC | 4 cut(s) | 400, 1710, 1818, 2490 |
| BbvI | GCAGC | 3 cut(s) | 1006, 2250, 2360 |
| BceAI | ACGGC | 1 cut(s) | 1472 |
| BciT130I | CCWGG | 1 cut(s) | 2640 |
| BclI | TGATCA | 2 cut(s) | 148, 1237 |
| BcnI | CCSGG | 2 cut(s) | 364, 2577 |
| BcoDI | GTCTC | 8 cut(s) | 9, 1715, 1905, 2222, 2231, 2374, 2444, 3091 |
| BfaI | CTAG | 9 cut(s) | 516, 552, 611, 1116, 1223, 2018, 2291, 2384, 2406 |
| BfmI | CTRYAG | 4 cut(s) | 43, 657, 972, 2374 |
| BfoI | RGCGCY | 1 cut(s) | 1867 |
| BfrI | CTTAAG | 1 cut(s) | 475 |
| BisI | GCNGC | 3 cut(s) | 995, 2239, 2374 |
| BlsI | GCNGC | 3 cut(s) | 996, 2240, 2375 |
| BmcAI | AGTACT | 2 cut(s) | 876, 1260 |
| Bme1390I | CCNGG | 3 cut(s) | 364, 2577, 2640 |
| Bme18I | GGWCC | 1 cut(s) | 653 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 2634 |
| BmeT110I | CYCGRG | 1 cut(s) | 61 |
| BmgT120I | GGNCC | 2 cut(s) | 74, 653 |
| BmiI | GGNNCC | 3 cut(s) | 339, 377, 2926 |
| BmrFI | CCNGG | 3 cut(s) | 364, 2577, 2640 |
| BmsI | GCATC | 7 cut(s) | 2014, 2090, 2099, 2340, 2396, 2693, 2958 |
| BpiI | GAAGAC | 6 cut(s) | 49, 474, 1148, 1315, 1772, 2997 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1768, 1800 |
| BpmI | CTGGAG | 3 cut(s) | 483, 1518, 1967 |
| Bpu14I | TTCGAA | 1 cut(s) | 2880 |
| BpuEI | CTTGAG | 4 cut(s) | 728, 1862, 2098, 2237 |
| BpuMI | CCSGG | 2 cut(s) | 364, 2577 |
| Bsa29I | ATCGAT | 1 cut(s) | 2847 |
| BsaAI | YACGTR | 2 cut(s) | 1024, 1181 |
| BsaBI | GATNNNNATC | 3 cut(s) | 218, 320, 1344 |
| BsaI | GGTCTC | 1 cut(s) | 2444 |
| BsaJI | CCNNGG | 4 cut(s) | 70, 363, 1505, 2608 |
| BsaWI | WCCGGW | 1 cut(s) | 1921 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1712, 1742 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 1150, 2645, 2680 |
| Bse1I | ACTGG | 1 cut(s) | 2132 |
| Bse21I | CCTNAGG | 1 cut(s) | 1716 |
| Bse3DI | GCAATG | 3 cut(s) | 456, 1702, 2390 |
| Bse8I | GATNNNNATC | 3 cut(s) | 218, 320, 1344 |
| BseAI | TCCGGA | 1 cut(s) | 1921 |
| BseBI | CCWGG | 1 cut(s) | 2640 |
| BseCI | ATCGAT | 1 cut(s) | 2847 |
| BseDI | CCNNGG | 4 cut(s) | 70, 363, 1505, 2608 |
| BseGI | GGATG | 5 cut(s) | 271, 451, 1991, 2081, 2114 |
| BseJI | GATNNNNATC | 3 cut(s) | 218, 320, 1344 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 1150, 2645, 2680 |
| BseMI | GCAATG | 3 cut(s) | 456, 1702, 2390 |
| BseMII | CTCAG | 4 cut(s) | 1276, 1707, 1952, 2242 |
| BseNI | ACTGG | 1 cut(s) | 2132 |
| BseRI | GAGGAG | 1 cut(s) | 1733 |
| BseSI | GKGCMC | 2 cut(s) | 400, 1710 |
| BseXI | GCAGC | 3 cut(s) | 1006, 2250, 2360 |
| Bsh1236I | CGCG | 1 cut(s) | 1735 |
| Bsh1285I | CGRYCG | 1 cut(s) | 222 |
| BshFI | GGCC | 2 cut(s) | 76, 2517 |
| BshVI | ATCGAT | 1 cut(s) | 2847 |
| BsiEI | CGRYCG | 1 cut(s) | 222 |
| BsiHKAI | GWGCWC | 4 cut(s) | 400, 1710, 1818, 2490 |
| BsiHKCI | CYCGRG | 1 cut(s) | 61 |
| BsiSI | CCGG | 4 cut(s) | 363, 1584, 1922, 2576 |
| BsiWI | CGTACG | 1 cut(s) | 2776 |
| BslFI | GGGAC | 2 cut(s) | 318, 588 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 1150, 2645, 2680 |
| BsmAI | GTCTC | 8 cut(s) | 9, 1715, 1905, 2222, 2231, 2374, 2444, 3091 |
| BsmBI | CGTCTC | 3 cut(s) | 1905, 2222, 2231 |
| BsmFI | GGGAC | 2 cut(s) | 318, 588 |
| BsmI | GAATGC | 2 cut(s) | 544, 2193 |
| BsnI | GGCC | 2 cut(s) | 76, 2517 |
| Bso31I | GGTCTC | 1 cut(s) | 2444 |
| BsoBI | CYCGRG | 1 cut(s) | 61 |
| Bsp119I | TTCGAA | 1 cut(s) | 2880 |
| Bsp1286I | GDGCHC | 4 cut(s) | 400, 1710, 1818, 2490 |
| Bsp13I | TCCGGA | 1 cut(s) | 1921 |
| Bsp1407I | TGTACA | 1 cut(s) | 2539 |
| BspACI | CCGC | 3 cut(s) | 1629, 1733, 2594 |
| BspANI | GGCC | 2 cut(s) | 76, 2517 |
| BspCNI | CTCAG | 4 cut(s) | 1275, 1708, 1953, 2241 |
| BspDI | ATCGAT | 1 cut(s) | 2847 |
| BspEI | TCCGGA | 1 cut(s) | 1921 |
| BspFNI | CGCG | 1 cut(s) | 1735 |
| BspLI | GGNNCC | 3 cut(s) | 339, 377, 2926 |
| BspMAI | CTGCAG | 2 cut(s) | 661, 2378 |
| BspPI | GGATC | 7 cut(s) | 140, 221, 1447, 1932, 1965, 1981, 2843 |
| BspT104I | TTCGAA | 1 cut(s) | 2880 |
| BspTI | CTTAAG | 1 cut(s) | 475 |
| BspTNI | GGTCTC | 1 cut(s) | 2444 |
| BsrDI | GCAATG | 3 cut(s) | 456, 1702, 2390 |
| BsrGI | TGTACA | 1 cut(s) | 2539 |
| BsrI | ACTGG | 1 cut(s) | 2132 |
| BssECI | CCNNGG | 4 cut(s) | 70, 363, 1505, 2608 |
| BssT1I | CCWWGG | 2 cut(s) | 1505, 2608 |
| Bst2UI | CCWGG | 1 cut(s) | 2640 |
| Bst4CI | ACNGT | 7 cut(s) | 117, 792, 2095, 2160, 2455, 3008, 3044 |
| Bst6I | CTCTTC | 5 cut(s) | 165, 1114, 1191, 2346, 2575 |
| BstAFI | CTTAAG | 1 cut(s) | 475 |
| BstAUI | TGTACA | 1 cut(s) | 2539 |
| BstBAI | YACGTR | 2 cut(s) | 1024, 1181 |
| BstBI | TTCGAA | 1 cut(s) | 2880 |
| BstC8I | GCNNGC | 6 cut(s) | 866, 1113, 1220, 1835, 2519, 2523 |
| BstDSI | CCRYGG | 1 cut(s) | 70 |
| BstEII | GGTNACC | 2 cut(s) | 1139, 3008 |
| BstF5I | GGATG | 5 cut(s) | 271, 451, 1991, 2081, 2114 |
| BstFNI | CGCG | 1 cut(s) | 1735 |
| BstH2I | RGCGCY | 1 cut(s) | 1867 |
| BstHHI | GCGC | 2 cut(s) | 111, 1866 |
| BstMAI | GTCTC | 8 cut(s) | 9, 1715, 1905, 2222, 2231, 2374, 2444, 3091 |
| BstMCI | CGRYCG | 1 cut(s) | 222 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 1822, 1843, 2531 |
| BstNI | CCWGG | 1 cut(s) | 2640 |
| BstNSI | RCATGY | 2 cut(s) | 109, 1532 |
| BstPI | GGTNACC | 2 cut(s) | 1139, 3008 |
| BstSCI | CCNGG | 3 cut(s) | 362, 2575, 2638 |
| BstSFI | CTRYAG | 4 cut(s) | 43, 657, 972, 2374 |
| BstSLI | GKGCMC | 2 cut(s) | 400, 1710 |
| BstUI | CGCG | 1 cut(s) | 1735 |
| BstV1I | GCAGC | 3 cut(s) | 1006, 2250, 2360 |
| BstV2I | GAAGAC | 6 cut(s) | 49, 474, 1148, 1315, 1772, 2997 |
| BstX2I | RGATCY | 4 cut(s) | 213, 1924, 1957, 1986 |
| BstYI | RGATCY | 4 cut(s) | 213, 1924, 1957, 1986 |
| Bsu15I | ATCGAT | 1 cut(s) | 2847 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1716 |
| BsuRI | GGCC | 2 cut(s) | 76, 2517 |
| BsuTUI | ATCGAT | 1 cut(s) | 2847 |
| BtgI | CCRYGG | 1 cut(s) | 70 |
| BtgZI | GCGATG | 1 cut(s) | 2670 |
| BtsCI | GGATG | 5 cut(s) | 271, 451, 1991, 2081, 2114 |
| BtsI | GCAGTG | 1 cut(s) | 2248 |
| BtsIMutI | CAGTG | 3 cut(s) | 1365, 2248, 3040 |
| Cac8I | GCNNGC | 6 cut(s) | 866, 1113, 1220, 1835, 2519, 2523 |
| CfoI | GCGC | 2 cut(s) | 111, 1866 |
| Cfr13I | GGNCC | 2 cut(s) | 74, 653 |
| ClaI | ATCGAT | 1 cut(s) | 2847 |
| CseI | GACGC | 2 cut(s) | 1724, 1887 |
| Csp6I | GTAC | 9 cut(s) | 179, 350, 875, 1018, 1259, 1569, 2540, 2701, 2777 |
| CviQI | GTAC | 9 cut(s) | 179, 350, 875, 1018, 1259, 1569, 2540, 2701, 2777 |
| DraI | TTTAAA | 3 cut(s) | 1642, 1678, 2283 |
| DraIII | CACNNNGTG | 1 cut(s) | 313 |
| DriI | GACNNNNNGTC | 1 cut(s) | 2634 |
| Eam1104I | CTCTTC | 5 cut(s) | 165, 1114, 1191, 2346, 2575 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 2634 |
| EarI | CTCTTC | 5 cut(s) | 165, 1114, 1191, 2346, 2575 |
| Ecl136II | GAGCTC | 2 cut(s) | 1816, 2488 |
| Eco130I | CCWWGG | 2 cut(s) | 1505, 2608 |
| Eco24I | GRGCYC | 2 cut(s) | 1818, 2490 |
| Eco31I | GGTCTC | 1 cut(s) | 2444 |
| Eco32I | GATATC | 2 cut(s) | 1249, 1384 |
| Eco47I | GGWCC | 1 cut(s) | 653 |
| Eco53kI | GAGCTC | 2 cut(s) | 1816, 2488 |
| Eco57I | CTGAAG | 1 cut(s) | 2877 |
| Eco72I | CACGTG | 1 cut(s) | 1024 |
| Eco81I | CCTNAGG | 1 cut(s) | 1716 |
| Eco88I | CYCGRG | 1 cut(s) | 61 |
| Eco91I | GGTNACC | 2 cut(s) | 1139, 3008 |
| EcoICRI | GAGCTC | 2 cut(s) | 1816, 2488 |
| EcoO65I | GGTNACC | 2 cut(s) | 1139, 3008 |
| EcoRI | GAATTC | 3 cut(s) | 368, 2597, 2882 |
| EcoRII | CCWGG | 1 cut(s) | 2638 |
| EcoRV | GATATC | 2 cut(s) | 1249, 1384 |
| EcoT14I | CCWWGG | 2 cut(s) | 1505, 2608 |
| EcoT38I | GRGCYC | 2 cut(s) | 1818, 2490 |
| ErhI | CCWWGG | 2 cut(s) | 1505, 2608 |
| Esp3I | CGTCTC | 3 cut(s) | 1905, 2222, 2231 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 737, 769 |
| FaqI | GGGAC | 2 cut(s) | 318, 588 |
| FbaI | TGATCA | 2 cut(s) | 148, 1237 |
| FblI | GTMKAC | 2 cut(s) | 700, 1480 |
| Fnu4HI | GCNGC | 3 cut(s) | 995, 2239, 2374 |
| FokI | GGATG | 5 cut(s) | 278, 458, 1978, 2068, 2121 |
| FriOI | GRGCYC | 2 cut(s) | 1818, 2490 |
| Fsp4HI | GCNGC | 3 cut(s) | 995, 2239, 2374 |
| FspBI | CTAG | 9 cut(s) | 516, 552, 611, 1116, 1223, 2018, 2291, 2384, 2406 |
| FspI | TGCGCA | 1 cut(s) | 110 |
| GlaI | GCGC | 2 cut(s) | 110, 1865 |
| GluI | GCNGC | 3 cut(s) | 995, 2239, 2374 |
| GsuI | CTGGAG | 3 cut(s) | 483, 1518, 1967 |
| HaeII | RGCGCY | 1 cut(s) | 1867 |
| HaeIII | GGCC | 2 cut(s) | 76, 2517 |
| HapII | CCGG | 4 cut(s) | 363, 1584, 1922, 2576 |
| HgaI | GACGC | 2 cut(s) | 1724, 1887 |
| HhaI | GCGC | 2 cut(s) | 111, 1866 |
| Hin6I | GCGC | 2 cut(s) | 109, 1864 |
| HinP1I | GCGC | 2 cut(s) | 109, 1864 |
| HincII | GTYRAC | 2 cut(s) | 701, 2142 |
| HindII | GTYRAC | 2 cut(s) | 701, 2142 |
| HindIII | AAGCTT | 6 cut(s) | 435, 751, 1047, 1109, 1130, 3079 |
| HpaII | CCGG | 4 cut(s) | 363, 1584, 1922, 2576 |
| HphI | GGTGA | 5 cut(s) | 1355, 2488, 2597, 2819, 3020 |
| Hpy188I | TCNGA | 8 cut(s) | 346, 529, 1039, 1265, 1962, 2308, 2857, 3077 |
| Hpy99I | CGWCG | 4 cut(s) | 1740, 2730, 2733, 2945 |
| HpyAV | CCTTC | 8 cut(s) | 30, 192, 436, 558, 1154, 1847, 1906, 2177 |
| HpyCH4III | ACNGT | 7 cut(s) | 117, 792, 2095, 2160, 2455, 3008, 3044 |
| HpyCH4IV | ACGT | 6 cut(s) | 392, 1023, 1180, 2224, 2775, 3072 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 1822, 1843, 2531 |
| HpySE526I | ACGT | 6 cut(s) | 392, 1023, 1180, 2224, 2775, 3072 |
| HspAI | GCGC | 2 cut(s) | 109, 1864 |
| Kpn2I | TCCGGA | 1 cut(s) | 1921 |
| Ksp22I | TGATCA | 2 cut(s) | 148, 1237 |
| LmnI | GCTCC | 5 cut(s) | 337, 429, 1537, 2924, 2946 |
| Lsp1109I | GCAGC | 3 cut(s) | 1006, 2250, 2360 |
| LweI | GCATC | 7 cut(s) | 2014, 2090, 2099, 2340, 2396, 2693, 2958 |
| MaeI | CTAG | 9 cut(s) | 516, 552, 611, 1116, 1223, 2018, 2291, 2384, 2406 |
| MaeII | ACGT | 6 cut(s) | 392, 1023, 1180, 2224, 2775, 3072 |
| MaeIII | GTNAC | 6 cut(s) | 101, 967, 1123, 1139, 1746, 3008 |
| MfeI | CAATTG | 2 cut(s) | 678, 2535 |
| MflI | RGATCY | 4 cut(s) | 213, 1924, 1957, 1986 |
| MhlI | GDGCHC | 4 cut(s) | 400, 1710, 1818, 2490 |
| MlyI | GAGTC | 4 cut(s) | 611, 696, 1275, 1786 |
| MmeI | TCCRAC | 3 cut(s) | 622, 2007, 3005 |
| MroI | TCCGGA | 1 cut(s) | 1921 |
| MroXI | GAANNNNTTC | 3 cut(s) | 753, 792, 2319 |
| MslI | CAYNNNNRTG | 5 cut(s) | 314, 384, 840, 1768, 2907 |
| MspA1I | CMGCKG | 1 cut(s) | 2594 |
| MspCI | CTTAAG | 1 cut(s) | 475 |
| MspI | CCGG | 4 cut(s) | 363, 1584, 1922, 2576 |
| MspR9I | CCNGG | 3 cut(s) | 364, 2577, 2640 |
| MunI | CAATTG | 2 cut(s) | 678, 2535 |
| Mva1269I | GAATGC | 2 cut(s) | 544, 2193 |
| MvaI | CCWGG | 1 cut(s) | 2640 |
| MvnI | CGCG | 1 cut(s) | 1735 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 1822, 1843, 2531 |
| NciI | CCSGG | 2 cut(s) | 364, 2577 |
| NlaIV | GGNNCC | 3 cut(s) | 339, 377, 2926 |
| NmuCI | GTSAC | 2 cut(s) | 101, 3008 |
| NsbI | TGCGCA | 1 cut(s) | 110 |
| NspI | RCATGY | 2 cut(s) | 109, 1532 |
| NspV | TTCGAA | 1 cut(s) | 2880 |
| PaeR7I | CTCGAG | 1 cut(s) | 61 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 215, 2221 |
| PctI | GAATGC | 2 cut(s) | 544, 2193 |
| PdmI | GAANNNNTTC | 3 cut(s) | 753, 792, 2319 |
| Pfl23II | CGTACG | 1 cut(s) | 2776 |
| PkrI | GCNGC | 3 cut(s) | 996, 2240, 2375 |
| Ple19I | CGATCG | 1 cut(s) | 222 |
| PleI | GAGTC | 4 cut(s) | 610, 696, 1274, 1785 |
| PmaCI | CACGTG | 1 cut(s) | 1024 |
| PmlI | CACGTG | 1 cut(s) | 1024 |
| PpsI | GAGTC | 4 cut(s) | 610, 696, 1274, 1785 |
| Ppu21I | YACGTR | 2 cut(s) | 1024, 1181 |
| PsiI | TTATAA | 1 cut(s) | 1625 |
| Psp124BI | GAGCTC | 2 cut(s) | 1818, 2490 |
| Psp6I | CCWGG | 1 cut(s) | 2638 |
| PspCI | CACGTG | 1 cut(s) | 1024 |
| PspEI | GGTNACC | 2 cut(s) | 1139, 3008 |
| PspGI | CCWGG | 1 cut(s) | 2638 |
| PspLI | CGTACG | 1 cut(s) | 2776 |
| PspN4I | GGNNCC | 3 cut(s) | 339, 377, 2926 |
| PspPI | GGNCC | 2 cut(s) | 74, 653 |
| PstI | CTGCAG | 2 cut(s) | 661, 2378 |
| PsuI | RGATCY | 4 cut(s) | 213, 1924, 1957, 1986 |
| PvuI | CGATCG | 1 cut(s) | 222 |
| RsaI | GTAC | 9 cut(s) | 180, 351, 876, 1019, 1260, 1570, 2541, 2702, 2778 |
| RsaNI | GTAC | 9 cut(s) | 179, 350, 875, 1018, 1259, 1569, 2540, 2701, 2777 |
| RseI | CAYNNNNRTG | 5 cut(s) | 314, 384, 840, 1768, 2907 |
| SacI | GAGCTC | 2 cut(s) | 1818, 2490 |
| SalI | GTCGAC | 1 cut(s) | 699 |
| SatI | GCNGC | 3 cut(s) | 995, 2239, 2374 |
| Sau96I | GGNCC | 2 cut(s) | 74, 653 |
| SbfI | CCTGCAGG | 1 cut(s) | 661 |
| ScaI | AGTACT | 2 cut(s) | 876, 1260 |
| SchI | GAGTC | 4 cut(s) | 611, 696, 1275, 1786 |
| ScrFI | CCNGG | 3 cut(s) | 364, 2577, 2640 |
| SdaI | CCTGCAGG | 1 cut(s) | 661 |
| SduI | GDGCHC | 4 cut(s) | 400, 1710, 1818, 2490 |
| SfaNI | GCATC | 7 cut(s) | 2014, 2090, 2099, 2340, 2396, 2693, 2958 |
| SfcI | CTRYAG | 4 cut(s) | 43, 657, 972, 2374 |
| Sfr274I | CTCGAG | 1 cut(s) | 61 |
| SfuI | TTCGAA | 1 cut(s) | 2880 |
| SinI | GGWCC | 1 cut(s) | 653 |
| SlaI | CTCGAG | 1 cut(s) | 61 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 314, 384, 840, 1768, 2907 |
| SmlI | CTYRAG | 6 cut(s) | 61, 475, 743, 1877, 2113, 2252 |
| SmoI | CTYRAG | 6 cut(s) | 61, 475, 743, 1877, 2113, 2252 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 661 |
| SsiI | CCGC | 3 cut(s) | 1629, 1733, 2594 |
| SspI | AATATT | 1 cut(s) | 2128 |
| SspMI | CTAG | 9 cut(s) | 516, 552, 611, 1116, 1223, 2018, 2291, 2384, 2406 |
| SstI | GAGCTC | 2 cut(s) | 1818, 2490 |
| StyD4I | CCNGG | 3 cut(s) | 362, 2575, 2638 |
| StyI | CCWWGG | 2 cut(s) | 1505, 2608 |
| TaaI | ACNGT | 7 cut(s) | 117, 792, 2095, 2160, 2455, 3008, 3044 |
| TaiI | ACGT | 6 cut(s) | 395, 1026, 1183, 2227, 2778, 3075 |
| TaqII | GACCGA | 1 cut(s) | 2954 |
| TatI | WGTACW | 4 cut(s) | 874, 1258, 2539, 2700 |
| TscAI | CASTG | 3 cut(s) | 1372, 2248, 3047 |
| TseFI | GTSAC | 2 cut(s) | 101, 3008 |
| TseI | GCWGC | 3 cut(s) | 994, 2238, 2373 |
| Tsp45I | GTSAC | 2 cut(s) | 101, 3008 |
| TspGWI | ACGGA | 5 cut(s) | 345, 367, 422, 2185, 2816 |
| TspRI | CASTG | 3 cut(s) | 1372, 2248, 3047 |
| Vha464I | CTTAAG | 1 cut(s) | 475 |
| VneI | GTGCAC | 2 cut(s) | 396, 1706 |
| VpaK11BI | GGWCC | 1 cut(s) | 653 |
| XapI | RAATTY | 5 cut(s) | 368, 1888, 2597, 2882, 2894 |
| XbaI | TCTAGA | 1 cut(s) | 551 |
| XceI | RCATGY | 2 cut(s) | 109, 1532 |
| XhoI | CTCGAG | 1 cut(s) | 61 |
| XmiI | GTMKAC | 2 cut(s) | 700, 1480 |
| XmnI | GAANNNNTTC | 3 cut(s) | 753, 792, 2319 |
| XspI | CTAG | 9 cut(s) | 516, 552, 611, 1116, 1223, 2018, 2291, 2384, 2406 |
| ZrmI | AGTACT | 2 cut(s) | 876, 1260 |