negative regulation of Rho guanyl-nucleotide exchange factor activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 101 |
| AccI | GTMKAC | 3 cut(s) | 726, 1587, 1651 |
| AccII | CGCG | 1 cut(s) | 2973 |
| AccIII | TCCGGA | 1 cut(s) | 1711 |
| AciI | CCGC | 3 cut(s) | 1778, 1942, 2971 |
| AclWI | GGATC | 9 cut(s) | 212, 374, 959, 2626, 2671, 2677, 2731, 2809, 2848 |
| AcoI | YGGCCR | 2 cut(s) | 441, 2471 |
| AcsI | RAATTY | 6 cut(s) | 359, 1703, 1801, 1994, 2198, 2417 |
| AcuI | CTGAAG | 3 cut(s) | 2838, 2877, 2916 |
| AfaI | GTAC | 3 cut(s) | 342, 1173, 1757 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1013 |
| AflII | CTTAAG | 1 cut(s) | 466 |
| AflIII | ACRYGT | 1 cut(s) | 1491 |
| AhdI | GACNNNNNGTC | 2 cut(s) | 71, 2812 |
| AjiI | CACGTC | 1 cut(s) | 1649 |
| AjnI | CCWGG | 1 cut(s) | 2500 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1259, 1291 |
| Alw21I | GWGCWC | 3 cut(s) | 391, 870, 2316 |
| Alw26I | GTCTC | 4 cut(s) | 9, 1076, 2053, 2357 |
| Alw44I | GTGCAC | 1 cut(s) | 387 |
| AlwI | GGATC | 9 cut(s) | 212, 374, 959, 2626, 2671, 2677, 2731, 2809, 2848 |
| Ama87I | CYCGRG | 2 cut(s) | 52, 1751 |
| Aor13HI | TCCGGA | 1 cut(s) | 1711 |
| AoxI | GGCC | 4 cut(s) | 441, 2189, 2399, 2471 |
| ApaLI | GTGCAC | 1 cut(s) | 387 |
| ApeKI | GCWGC | 3 cut(s) | 985, 1134, 2154 |
| ApoI | RAATTY | 6 cut(s) | 359, 1703, 1801, 1994, 2198, 2417 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 30, 62 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 783, 1971, 2232 |
| AspLEI | GCGC | 3 cut(s) | 102, 282, 2159 |
| AspS9I | GGNCC | 7 cut(s) | 65, 644, 889, 938, 1812, 2400, 2440 |
| AsuHPI | GGTGA | 3 cut(s) | 1298, 2413, 2522 |
| AsuII | TTCGAA | 1 cut(s) | 2415 |
| AvaI | CYCGRG | 2 cut(s) | 52, 1751 |
| AvaII | GGWCC | 6 cut(s) | 65, 644, 889, 938, 1812, 2440 |
| BaeGI | GKGCMC | 1 cut(s) | 391 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 2206, 2239 |
| BalI | TGGCCA | 1 cut(s) | 443 |
| BanII | GRGCYC | 1 cut(s) | 2316 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 2530, 2562 |
| BauI | CACGAG | 2 cut(s) | 1446, 2938 |
| BbsI | GAAGAC | 7 cut(s) | 40, 307, 465, 2299, 2751, 2865, 2904 |
| Bbv12I | GWGCWC | 3 cut(s) | 391, 870, 2316 |
| BbvCI | CCTCAGC | 1 cut(s) | 1946 |
| BbvI | GCAGC | 3 cut(s) | 997, 1146, 2166 |
| BccI | CCATC | 9 cut(s) | 611, 622, 803, 1110, 1598, 1630, 1716, 1852, 1912 |
| BceAI | ACGGC | 3 cut(s) | 136, 571, 2486 |
| BciT130I | CCWGG | 1 cut(s) | 2502 |
| BciVI | GTATCC | 1 cut(s) | 1653 |
| BclI | TGATCA | 1 cut(s) | 139 |
| BcoDI | GTCTC | 4 cut(s) | 9, 1076, 2053, 2357 |
| BfaI | CTAG | 5 cut(s) | 602, 758, 1139, 2204, 2336 |
| BfmI | CTRYAG | 4 cut(s) | 648, 683, 983, 1132 |
| BfrI | CTTAAG | 1 cut(s) | 466 |
| BfuI | GTATCC | 1 cut(s) | 1653 |
| BglII | AGATCT | 2 cut(s) | 1328, 2976 |
| BisI | GCNGC | 6 cut(s) | 986, 1135, 1778, 1943, 2155, 2971 |
| BlsI | GCNGC | 6 cut(s) | 987, 1136, 1779, 1944, 2156, 2972 |
| BmcAI | AGTACT | 1 cut(s) | 1173 |
| Bme1390I | CCNGG | 1 cut(s) | 2502 |
| Bme18I | GGWCC | 6 cut(s) | 65, 644, 889, 938, 1812, 2440 |
| BmeRI | GACNNNNNGTC | 2 cut(s) | 71, 2812 |
| BmeT110I | CYCGRG | 2 cut(s) | 52, 1751 |
| BmgBI | CACGTC | 1 cut(s) | 1649 |
| BmgT120I | GGNCC | 7 cut(s) | 65, 644, 889, 938, 1812, 2400, 2440 |
| BmiI | GGNNCC | 5 cut(s) | 330, 347, 1577, 1771, 1813 |
| BmrFI | CCNGG | 1 cut(s) | 2502 |
| BpiI | GAAGAC | 7 cut(s) | 40, 307, 465, 2299, 2751, 2865, 2904 |
| BpmI | CTGGAG | 2 cut(s) | 474, 2381 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1946, 2210 |
| Bpu14I | TTCGAA | 1 cut(s) | 2415 |
| BpuEI | CTTGAG | 4 cut(s) | 1340, 1775, 2011, 2312 |
| Bsa29I | ATCGAT | 1 cut(s) | 1524 |
| BsaAI | YACGTR | 3 cut(s) | 392, 1097, 1492 |
| BsaBI | GATNNNNATC | 2 cut(s) | 209, 2668 |
| BsaI | GGTCTC | 2 cut(s) | 1076, 2357 |
| BsaJI | CCNNGG | 2 cut(s) | 2186, 2533 |
| BsaWI | WCCGGW | 3 cut(s) | 67, 353, 1711 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2615, 2645 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1013 |
| Bse1I | ACTGG | 2 cut(s) | 2045, 2364 |
| Bse3DI | GCAATG | 1 cut(s) | 2303 |
| Bse8I | GATNNNNATC | 2 cut(s) | 209, 2668 |
| BseAI | TCCGGA | 1 cut(s) | 1711 |
| BseBI | CCWGG | 1 cut(s) | 2502 |
| BseCI | ATCGAT | 1 cut(s) | 1524 |
| BseDI | CCNNGG | 2 cut(s) | 2186, 2533 |
| BseJI | GATNNNNATC | 2 cut(s) | 209, 2668 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1013 |
| BseMI | GCAATG | 1 cut(s) | 2303 |
| BseMII | CTCAG | 4 cut(s) | 851, 1865, 1937, 2970 |
| BseNI | ACTGG | 2 cut(s) | 2045, 2364 |
| BseSI | GKGCMC | 1 cut(s) | 391 |
| BseXI | GCAGC | 3 cut(s) | 997, 1146, 2166 |
| BseYI | CCCAGC | 1 cut(s) | 1773 |
| Bsh1236I | CGCG | 1 cut(s) | 2973 |
| Bsh1285I | CGRYCG | 1 cut(s) | 213 |
| BshFI | GGCC | 4 cut(s) | 443, 2191, 2401, 2473 |
| BshVI | ATCGAT | 1 cut(s) | 1524 |
| BsiEI | CGRYCG | 1 cut(s) | 213 |
| BsiHKAI | GWGCWC | 3 cut(s) | 391, 870, 2316 |
| BsiHKCI | CYCGRG | 2 cut(s) | 52, 1751 |
| BsiSI | CCGG | 3 cut(s) | 68, 354, 1712 |
| BslFI | GGGAC | 2 cut(s) | 579, 2500 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1013 |
| BsmAI | GTCTC | 4 cut(s) | 9, 1076, 2053, 2357 |
| BsmFI | GGGAC | 2 cut(s) | 579, 2500 |
| BsmI | GAATGC | 1 cut(s) | 535 |
| BsnI | GGCC | 4 cut(s) | 443, 2191, 2401, 2473 |
| Bso31I | GGTCTC | 2 cut(s) | 1076, 2357 |
| BsoBI | CYCGRG | 2 cut(s) | 52, 1751 |
| Bsp119I | TTCGAA | 1 cut(s) | 2415 |
| Bsp1286I | GDGCHC | 3 cut(s) | 391, 870, 2316 |
| Bsp13I | TCCGGA | 1 cut(s) | 1711 |
| BspACI | CCGC | 3 cut(s) | 1778, 1942, 2971 |
| BspANI | GGCC | 4 cut(s) | 443, 2191, 2401, 2473 |
| BspCNI | CTCAG | 4 cut(s) | 852, 1866, 1938, 2969 |
| BspDI | ATCGAT | 1 cut(s) | 1524 |
| BspEI | TCCGGA | 1 cut(s) | 1711 |
| BspFNI | CGCG | 1 cut(s) | 2973 |
| BspLI | GGNNCC | 5 cut(s) | 330, 347, 1577, 1771, 1813 |
| BspMAI | CTGCAG | 4 cut(s) | 652, 687, 987, 1136 |
| BspPI | GGATC | 9 cut(s) | 212, 374, 959, 2626, 2671, 2677, 2731, 2809, 2848 |
| BspQI | GCTCTTC | 1 cut(s) | 2449 |
| BspT104I | TTCGAA | 1 cut(s) | 2415 |
| BspTI | CTTAAG | 1 cut(s) | 466 |
| BspTNI | GGTCTC | 2 cut(s) | 1076, 2357 |
| BsrDI | GCAATG | 1 cut(s) | 2303 |
| BsrI | ACTGG | 2 cut(s) | 2045, 2364 |
| BssECI | CCNNGG | 2 cut(s) | 2186, 2533 |
| BssNAI | GTATAC | 1 cut(s) | 1588 |
| BssSI | CACGAG | 2 cut(s) | 1446, 2938 |
| BssT1I | CCWWGG | 1 cut(s) | 2186 |
| Bst1107I | GTATAC | 1 cut(s) | 1588 |
| Bst2BI | CACGAG | 2 cut(s) | 1446, 2938 |
| Bst2UI | CCWGG | 1 cut(s) | 2502 |
| Bst4CI | ACNGT | 6 cut(s) | 108, 313, 1286, 1585, 2368, 2429 |
| BstAFI | CTTAAG | 1 cut(s) | 466 |
| BstBAI | YACGTR | 3 cut(s) | 392, 1097, 1492 |
| BstBI | TTCGAA | 1 cut(s) | 2415 |
| BstC8I | GCNNGC | 5 cut(s) | 857, 1029, 1748, 2517, 2945 |
| BstFNI | CGCG | 1 cut(s) | 2973 |
| BstHHI | GCGC | 3 cut(s) | 102, 282, 2159 |
| BstMAI | GTCTC | 4 cut(s) | 9, 1076, 2053, 2357 |
| BstMCI | CGRYCG | 1 cut(s) | 213 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 449, 856, 865, 985, 2456 |
| BstNI | CCWGG | 1 cut(s) | 2502 |
| BstNSI | RCATGY | 1 cut(s) | 100 |
| BstSCI | CCNGG | 1 cut(s) | 2500 |
| BstSFI | CTRYAG | 4 cut(s) | 648, 683, 983, 1132 |
| BstSLI | GKGCMC | 1 cut(s) | 391 |
| BstUI | CGCG | 1 cut(s) | 2973 |
| BstV1I | GCAGC | 3 cut(s) | 997, 1146, 2166 |
| BstV2I | GAAGAC | 7 cut(s) | 40, 307, 465, 2299, 2751, 2865, 2904 |
| BstX2I | RGATCY | 4 cut(s) | 204, 1328, 2663, 2976 |
| BstYI | RGATCY | 4 cut(s) | 204, 1328, 2663, 2976 |
| BstZ17I | GTATAC | 1 cut(s) | 1588 |
| Bsu15I | ATCGAT | 1 cut(s) | 1524 |
| BsuI | GTATCC | 1 cut(s) | 1653 |
| BsuRI | GGCC | 4 cut(s) | 443, 2191, 2401, 2473 |
| BsuTUI | ATCGAT | 1 cut(s) | 1524 |
| BtrI | CACGTC | 1 cut(s) | 1649 |
| BtsIMutI | CAGTG | 1 cut(s) | 2357 |
| Cac8I | GCNNGC | 5 cut(s) | 857, 1029, 1748, 2517, 2945 |
| CfoI | GCGC | 3 cut(s) | 102, 282, 2159 |
| Cfr13I | GGNCC | 7 cut(s) | 65, 644, 889, 938, 1812, 2400, 2440 |
| ClaI | ATCGAT | 1 cut(s) | 1524 |
| CpoI | CGGWCCG | 1 cut(s) | 65 |
| CseI | GACGC | 1 cut(s) | 2724 |
| Csp6I | GTAC | 3 cut(s) | 341, 1172, 1756 |
| CspI | CGGWCCG | 1 cut(s) | 65 |
| CviAII | CATG | 5 cut(s) | 97, 559, 930, 1984, 2129 |
| CviQI | GTAC | 3 cut(s) | 341, 1172, 1756 |
| DraI | TTTAAA | 1 cut(s) | 1806 |
| DriI | GACNNNNNGTC | 2 cut(s) | 71, 2812 |
| EaeI | YGGCCR | 2 cut(s) | 441, 2471 |
| Eam1105I | GACNNNNNGTC | 2 cut(s) | 71, 2812 |
| Ecl136II | GAGCTC | 1 cut(s) | 2314 |
| Eco130I | CCWWGG | 1 cut(s) | 2186 |
| Eco147I | AGGCCT | 1 cut(s) | 2191 |
| Eco24I | GRGCYC | 1 cut(s) | 2316 |
| Eco31I | GGTCTC | 2 cut(s) | 1076, 2357 |
| Eco32I | GATATC | 3 cut(s) | 271, 1162, 1344 |
| Eco47I | GGWCC | 6 cut(s) | 65, 644, 889, 938, 1812, 2440 |
| Eco53kI | GAGCTC | 1 cut(s) | 2314 |
| Eco57I | CTGAAG | 3 cut(s) | 2838, 2877, 2916 |
| Eco88I | CYCGRG | 2 cut(s) | 52, 1751 |
| EcoICRI | GAGCTC | 1 cut(s) | 2314 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1812 |
| EcoRI | GAATTC | 2 cut(s) | 359, 2417 |
| EcoRII | CCWGG | 1 cut(s) | 2500 |
| EcoRV | GATATC | 3 cut(s) | 271, 1162, 1344 |
| EcoT14I | CCWWGG | 1 cut(s) | 2186 |
| EcoT22I | ATGCAT | 1 cut(s) | 238 |
| EcoT38I | GRGCYC | 1 cut(s) | 2316 |
| ErhI | CCWWGG | 1 cut(s) | 2186 |
| FaeI | CATG | 5 cut(s) | 100, 562, 933, 1987, 2132 |
| FaqI | GGGAC | 2 cut(s) | 579, 2500 |
| FatI | CATG | 5 cut(s) | 96, 558, 929, 1983, 2128 |
| FbaI | TGATCA | 1 cut(s) | 139 |
| FblI | GTMKAC | 3 cut(s) | 726, 1587, 1651 |
| Fnu4HI | GCNGC | 6 cut(s) | 986, 1135, 1778, 1943, 2155, 2971 |
| FriOI | GRGCYC | 1 cut(s) | 2316 |
| Fsp4HI | GCNGC | 6 cut(s) | 986, 1135, 1778, 1943, 2155, 2971 |
| FspBI | CTAG | 5 cut(s) | 602, 758, 1139, 2204, 2336 |
| FspI | TGCGCA | 1 cut(s) | 101 |
| GlaI | GCGC | 3 cut(s) | 101, 281, 2158 |
| GluI | GCNGC | 6 cut(s) | 986, 1135, 1778, 1943, 2155, 2971 |
| GsaI | CCCAGC | 1 cut(s) | 1777 |
| GsuI | CTGGAG | 2 cut(s) | 474, 2381 |
| HaeIII | GGCC | 4 cut(s) | 443, 2191, 2401, 2473 |
| HapII | CCGG | 3 cut(s) | 68, 354, 1712 |
| HgaI | GACGC | 1 cut(s) | 2724 |
| HhaI | GCGC | 3 cut(s) | 102, 282, 2159 |
| Hin1II | CATG | 5 cut(s) | 100, 562, 933, 1987, 2132 |
| Hin6I | GCGC | 3 cut(s) | 100, 280, 2157 |
| HinP1I | GCGC | 3 cut(s) | 100, 280, 2157 |
| HincII | GTYRAC | 3 cut(s) | 727, 1652, 2055 |
| HindII | GTYRAC | 3 cut(s) | 727, 1652, 2055 |
| HindIII | AAGCTT | 2 cut(s) | 426, 2682 |
| HpaII | CCGG | 3 cut(s) | 68, 354, 1712 |
| HphI | GGTGA | 3 cut(s) | 1298, 2413, 2522 |
| Hpy166II | GTNNAC | 8 cut(s) | 389, 727, 773, 889, 1058, 1588, 1652, 2055 |
| Hpy8I | GTNNAC | 8 cut(s) | 389, 727, 773, 889, 1058, 1588, 1652, 2055 |
| Hpy99I | CGWCG | 1 cut(s) | 1653 |
| HpyAV | CCTTC | 5 cut(s) | 183, 1106, 1819, 2413, 2699 |
| HpyCH4III | ACNGT | 6 cut(s) | 108, 313, 1286, 1585, 2368, 2429 |
| HpyCH4IV | ACGT | 6 cut(s) | 391, 1096, 1303, 1491, 1648, 2117 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 449, 856, 865, 985, 2456 |
| HpySE526I | ACGT | 6 cut(s) | 391, 1096, 1303, 1491, 1648, 2117 |
| Hsp92II | CATG | 5 cut(s) | 100, 562, 933, 1987, 2132 |
| HspAI | GCGC | 3 cut(s) | 100, 280, 2157 |
| Kpn2I | TCCGGA | 1 cut(s) | 1711 |
| Ksp22I | TGATCA | 1 cut(s) | 139 |
| LguI | GCTCTTC | 1 cut(s) | 2449 |
| LmnI | GCTCC | 5 cut(s) | 328, 420, 1547, 2319, 2709 |
| Lsp1109I | GCAGC | 3 cut(s) | 997, 1146, 2166 |
| MaeI | CTAG | 5 cut(s) | 602, 758, 1139, 2204, 2336 |
| MaeII | ACGT | 6 cut(s) | 391, 1096, 1303, 1491, 1648, 2117 |
| MaeIII | GTNAC | 5 cut(s) | 92, 958, 1039, 1286, 2131 |
| MflI | RGATCY | 4 cut(s) | 204, 1328, 2663, 2976 |
| MhlI | GDGCHC | 3 cut(s) | 391, 870, 2316 |
| MlsI | TGGCCA | 1 cut(s) | 443 |
| MluNI | TGGCCA | 1 cut(s) | 443 |
| MlyI | GAGTC | 5 cut(s) | 602, 697, 2055, 2289, 2621 |
| MmeI | TCCRAC | 2 cut(s) | 613, 2491 |
| Mox20I | TGGCCA | 1 cut(s) | 443 |
| Mph1103I | ATGCAT | 1 cut(s) | 238 |
| MroI | TCCGGA | 1 cut(s) | 1711 |
| MroXI | GAANNNNTTC | 3 cut(s) | 783, 1971, 2232 |
| MscI | TGGCCA | 1 cut(s) | 443 |
| MslI | CAYNNNNRTG | 3 cut(s) | 128, 831, 2392 |
| Msp20I | TGGCCA | 1 cut(s) | 443 |
| MspA1I | CMGCKG | 2 cut(s) | 1979, 2090 |
| MspCI | CTTAAG | 1 cut(s) | 466 |
| MspI | CCGG | 3 cut(s) | 68, 354, 1712 |
| MspR9I | CCNGG | 1 cut(s) | 2502 |
| Mva1269I | GAATGC | 1 cut(s) | 535 |
| MvaI | CCWGG | 1 cut(s) | 2502 |
| MvnI | CGCG | 1 cut(s) | 2973 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 449, 856, 865, 985, 2456 |
| NlaIII | CATG | 5 cut(s) | 100, 562, 933, 1987, 2132 |
| NlaIV | GGNNCC | 5 cut(s) | 330, 347, 1577, 1771, 1813 |
| NmuCI | GTSAC | 2 cut(s) | 92, 1286 |
| NsbI | TGCGCA | 1 cut(s) | 101 |
| NsiI | ATGCAT | 1 cut(s) | 238 |
| NspI | RCATGY | 1 cut(s) | 100 |
| NspV | TTCGAA | 1 cut(s) | 2415 |
| PaeR7I | CTCGAG | 1 cut(s) | 1751 |
| PceI | AGGCCT | 1 cut(s) | 2191 |
| PciSI | GCTCTTC | 1 cut(s) | 2449 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 206, 2141 |
| PctI | GAATGC | 1 cut(s) | 535 |
| PdmI | GAANNNNTTC | 3 cut(s) | 783, 1971, 2232 |
| PkrI | GCNGC | 6 cut(s) | 987, 1136, 1779, 1944, 2156, 2972 |
| Ple19I | CGATCG | 1 cut(s) | 213 |
| PleI | GAGTC | 5 cut(s) | 601, 696, 2055, 2289, 2620 |
| PpsI | GAGTC | 5 cut(s) | 601, 696, 2055, 2289, 2620 |
| Ppu21I | YACGTR | 3 cut(s) | 392, 1097, 1492 |
| PpuMI | RGGWCCY | 1 cut(s) | 1812 |
| Psp124BI | GAGCTC | 1 cut(s) | 2316 |
| Psp5II | RGGWCCY | 1 cut(s) | 1812 |
| Psp6I | CCWGG | 1 cut(s) | 2500 |
| PspFI | CCCAGC | 1 cut(s) | 1773 |
| PspGI | CCWGG | 1 cut(s) | 2500 |
| PspN4I | GGNNCC | 5 cut(s) | 330, 347, 1577, 1771, 1813 |
| PspPI | GGNCC | 7 cut(s) | 65, 644, 889, 938, 1812, 2400, 2440 |
| PspPPI | RGGWCCY | 1 cut(s) | 1812 |
| PspXI | VCTCGAGB | 1 cut(s) | 1751 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1069, 1101 |
| PstI | CTGCAG | 4 cut(s) | 652, 687, 987, 1136 |
| PsuI | RGATCY | 4 cut(s) | 204, 1328, 2663, 2976 |
| PvuI | CGATCG | 1 cut(s) | 213 |
| PvuII | CAGCTG | 2 cut(s) | 1979, 2090 |
| RsaI | GTAC | 3 cut(s) | 342, 1173, 1757 |
| RsaNI | GTAC | 3 cut(s) | 341, 1172, 1756 |
| RseI | CAYNNNNRTG | 3 cut(s) | 128, 831, 2392 |
| Rsr2I | CGGWCCG | 1 cut(s) | 65 |
| RsrII | CGGWCCG | 1 cut(s) | 65 |
| SacI | GAGCTC | 1 cut(s) | 2316 |
| SalI | GTCGAC | 2 cut(s) | 725, 1650 |
| SapI | GCTCTTC | 1 cut(s) | 2449 |
| SatI | GCNGC | 6 cut(s) | 986, 1135, 1778, 1943, 2155, 2971 |
| Sau96I | GGNCC | 7 cut(s) | 65, 644, 889, 938, 1812, 2400, 2440 |
| SbfI | CCTGCAGG | 1 cut(s) | 652 |
| ScaI | AGTACT | 1 cut(s) | 1173 |
| SchI | GAGTC | 5 cut(s) | 602, 697, 2055, 2289, 2621 |
| ScrFI | CCNGG | 1 cut(s) | 2502 |
| SdaI | CCTGCAGG | 1 cut(s) | 652 |
| SduI | GDGCHC | 3 cut(s) | 391, 870, 2316 |
| SfcI | CTRYAG | 4 cut(s) | 648, 683, 983, 1132 |
| Sfr274I | CTCGAG | 1 cut(s) | 1751 |
| SfuI | TTCGAA | 1 cut(s) | 2415 |
| SinI | GGWCC | 6 cut(s) | 65, 644, 889, 938, 1812, 2440 |
| SlaI | CTCGAG | 1 cut(s) | 1751 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 128, 831, 2392 |
| SmlI | CTYRAG | 6 cut(s) | 466, 1319, 1751, 1790, 2026, 2327 |
| SmoI | CTYRAG | 6 cut(s) | 466, 1319, 1751, 1790, 2026, 2327 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 652 |
| SseBI | AGGCCT | 1 cut(s) | 2191 |
| SsiI | CCGC | 3 cut(s) | 1778, 1942, 2971 |
| SspI | AATATT | 3 cut(s) | 448, 1150, 2041 |
| SspMI | CTAG | 5 cut(s) | 602, 758, 1139, 2204, 2336 |
| SstI | GAGCTC | 1 cut(s) | 2316 |
| StuI | AGGCCT | 1 cut(s) | 2191 |
| StyD4I | CCNGG | 1 cut(s) | 2500 |
| StyI | CCWWGG | 1 cut(s) | 2186 |
| TaaI | ACNGT | 6 cut(s) | 108, 313, 1286, 1585, 2368, 2429 |
| TaiI | ACGT | 6 cut(s) | 394, 1099, 1306, 1494, 1651, 2120 |
| TatI | WGTACW | 1 cut(s) | 1171 |
| TauI | GCSGC | 3 cut(s) | 1780, 1945, 2973 |
| TscAI | CASTG | 1 cut(s) | 2364 |
| TseFI | GTSAC | 2 cut(s) | 92, 1286 |
| TseI | GCWGC | 3 cut(s) | 985, 1134, 2154 |
| Tsp45I | GTSAC | 2 cut(s) | 92, 1286 |
| TspGWI | ACGGA | 4 cut(s) | 65, 78, 358, 413 |
| TspRI | CASTG | 1 cut(s) | 2364 |
| Vha464I | CTTAAG | 1 cut(s) | 466 |
| VneI | GTGCAC | 1 cut(s) | 387 |
| VpaK11BI | GGWCC | 6 cut(s) | 65, 644, 889, 938, 1812, 2440 |
| XapI | RAATTY | 6 cut(s) | 359, 1703, 1801, 1994, 2198, 2417 |
| XbaI | TCTAGA | 2 cut(s) | 757, 2203 |
| XceI | RCATGY | 1 cut(s) | 100 |
| XhoI | CTCGAG | 1 cut(s) | 1751 |
| XmiI | GTMKAC | 3 cut(s) | 726, 1587, 1651 |
| XmnI | GAANNNNTTC | 3 cut(s) | 783, 1971, 2232 |
| XspI | CTAG | 5 cut(s) | 602, 758, 1139, 2204, 2336 |
| ZrmI | AGTACT | 1 cut(s) | 1173 |
| Zsp2I | ATGCAT | 1 cut(s) | 238 |