AT1G63470

DNA-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005616
Physical Location & Seq
Reverse (-)
23536466 .. 23539242
2777 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G63470.1

Sequence Viewer

Length: 1137 bp
ATGGATGGGAGAGAAGCTATGGCATTTCCAGGCTCGCATTCTCAGTTCTATCTTCAGAGAGGAGTTTTTACCAATCTTACACCTTCCCAGGTCGCGAGTGGTCTTCACGCGCCGCCGCCACCTCCGGGAATGAGGCCAATGTCAAACCCTAACATTCACCACCCTCAGGCTAGCAATCCAGGACCTCCTTTCTCCATGGCTGAGCACAGGCATTCTGATTTCGGACACAGCATTCACATGGGGATGGCTTCTCCTGCTGCGGTGCAGCCTACTCTGCAGCTGCCGCCTCCACCGTCGGAGCAACCGATGGTTAAGAAGAAACGTGGAAGGCCAAGAAAGTATGTTCCTGATGGACAAGTTTCATTGGGGCTTTCTCCAATGCCTTGTGTTAGTAAAAAGTCTAAGGACTCTTCTTCAATGTCTGATCCTAATGCACCTAAAAGAGCCAGAGGTCGACCTCCCGGAACTGGAAGGAAGCAACGCTTGGCTAATCTTGGTGAGTGGATGAATACATCAGCTGGACTTGCTTTTGCACCTCATGTGATCAGTGTTGGATCAGGAGAAGATATTGTTTCGAAAGTTTTGTCTTTTTCACAAAAAAGACCTCGGGCTCTTTGTATAATGTCAGGCACTGGAACGGTTTCTTCAGTCACTCTGCGTGAACCCGCTTCAACAACGCCTTCCTTAACATTTGAGGGACGTTTTGAGATTCTAAGTTTAGGTGGATCTTATCTGGTGAATGAAGAAGGTGGATCCAAAAGTCGAACAGGCGGTTTGAGTGTCTCTCTTTCTGGTCCTGAAGGTCATGTTATCGGCGGTGGAATTGGAATGCTTATCGCAGCCAGCCTCGTTCAGGTGGTGGCTTGTAGTTTCGTGTACGGAGCAAGTGCAAAGTCTAATAATAACAATAACAAGACCATCAAACAAGAAATAAAACCTAAGCAAGAGCCGACCAATAGCGAAATGGAGACCACACCTGGTAGTGCACCAGAAGCTGCAGCATCGACGGGTCAGCATACGCCACAGAACTTCCCAGCTCAGGGAATGAGCGGATGGCCCGTTTCAGGCTCAGGCTCAGGCAGATCACTTGACTCTAGCAGAAACCCACTCACTGATATTGACTTGACTCGCGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0043621
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

378

Amino Acids

39.64

Weight (kDa)

9.81

Isoelectric Point (pI)

54.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 179 - 291 1.1e-22 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1050
AccI GTMKAC 1 cut(s) 454
AccII CGCG 3 cut(s) 95, 110, 1131
AciI CCGC 9 cut(s) 113, 116, 260, 284, 666, 771, 816, 1050, 1131
AclWI GGATC 5 cut(s) 419, 562, 733, 747, 760
AcuI CTGAAG 3 cut(s) 38, 630, 819
AfaI GTAC 1 cut(s) 878
AfiI CCNNNNNNNGG 7 cut(s) 125, 166, 467, 853, 1039, 1040, 1064
AgsI TTSAA 2 cut(s) 417, 672
AjnI CCWGG 4 cut(s) 28, 87, 178, 976
AjuI GAANNNNNNNTTGG 2 cut(s) 467, 499
AluBI AGCT 5 cut(s) 17, 280, 518, 995, 1037
AluI AGCT 5 cut(s) 17, 280, 518, 995, 1037
Alw21I GWGCWC 2 cut(s) 207, 988
Alw26I GTCTC 2 cut(s) 787, 962
Alw44I GTGCAC 1 cut(s) 984
AlwI GGATC 5 cut(s) 419, 562, 733, 747, 760
AlwNI CAGNNNCTG 2 cut(s) 632, 995
Ama87I CYCGRG 1 cut(s) 606
AoxI GGCC 3 cut(s) 134, 329, 1055
ApaLI GTGCAC 1 cut(s) 984
ApeKI GCWGC 7 cut(s) 257, 265, 277, 280, 839, 995, 998
ArsI GACNNNNNNTTYG 2 cut(s) 569, 601
Asp700I GAANNNNTTC 1 cut(s) 640
AspLEI GCGC 1 cut(s) 112
AspS9I GGNCC 3 cut(s) 182, 794, 1056
AsuC2I CCSGG 2 cut(s) 126, 462
AsuHPI GGTGA 3 cut(s) 149, 509, 748
AsuII TTCGAA 1 cut(s) 575
AsuNHI GCTAGC 1 cut(s) 170
AvaI CYCGRG 1 cut(s) 606
AvaII GGWCC 2 cut(s) 182, 794
AxyI CCTNAGG 1 cut(s) 165
BaeGI GKGCMC 1 cut(s) 988
BamHI GGATCC 1 cut(s) 752
BanII GRGCYC 1 cut(s) 613
BbsI GAAGAC 1 cut(s) 95
Bbv12I GWGCWC 2 cut(s) 207, 988
BbvI GCAGC 7 cut(s) 244, 267, 277, 289, 851, 982, 1010
BccI CCATC 5 cut(s) 238, 301, 344, 926, 1047
BciT130I CCWGG 4 cut(s) 30, 89, 180, 978
BclI TGATCA 1 cut(s) 543
BcnI CCSGG 2 cut(s) 126, 462
BcoDI GTCTC 2 cut(s) 787, 962
BfaI CTAG 2 cut(s) 171, 1095
BfmI CTRYAG 2 cut(s) 275, 996
BlpI GCTNAGC 1 cut(s) 201
Bme1390I CCNGG 6 cut(s) 30, 89, 126, 180, 462, 978
Bme18I GGWCC 2 cut(s) 182, 794
BmeT110I CYCGRG 1 cut(s) 606
BmgT120I GGNCC 3 cut(s) 182, 794, 1056
BmiI GGNNCC 1 cut(s) 754
BmrFI CCNGG 6 cut(s) 30, 89, 126, 180, 462, 978
BmsI GCATC 1 cut(s) 1010
BmtI GCTAGC 1 cut(s) 174
BpiI GAAGAC 1 cut(s) 95
BplI GAGNNNNNCTC 2 cut(s) 769, 801
Bpu10I CCTNAGC 4 cut(s) 939, 1038, 1069, 1075
Bpu1102I GCTNAGC 1 cut(s) 201
Bpu14I TTCGAA 1 cut(s) 575
BpuMI CCSGG 2 cut(s) 126, 462
BsaI GGTCTC 1 cut(s) 962
BsaJI CCNNGG 3 cut(s) 87, 195, 605
Bsc4I CCNNNNNNNGG 7 cut(s) 125, 166, 467, 853, 1039, 1040, 1064
Bse1I ACTGG 2 cut(s) 472, 637
Bse21I CCTNAGG 1 cut(s) 165
BseBI CCWGG 4 cut(s) 30, 89, 180, 978
BseDI CCNNGG 3 cut(s) 87, 195, 605
BseGI GGATG 4 cut(s) 10, 249, 510, 1058
BseLI CCNNNNNNNGG 7 cut(s) 125, 166, 467, 853, 1039, 1040, 1064
BseMII CTCAG 6 cut(s) 56, 179, 192, 1052, 1083, 1089
BseNI ACTGG 2 cut(s) 472, 637
BseRI GAGGAG 1 cut(s) 75
BseSI GKGCMC 1 cut(s) 988
BseXI GCAGC 7 cut(s) 244, 267, 277, 289, 851, 982, 1010
BseYI CCCAGC 1 cut(s) 1033
BsgI GTGCAG 1 cut(s) 284
Bsh1236I CGCG 3 cut(s) 95, 110, 1131
BshFI GGCC 3 cut(s) 136, 331, 1057
BsiHKAI GWGCWC 2 cut(s) 207, 988
BsiHKCI CYCGRG 1 cut(s) 606
BsiSI CCGG 2 cut(s) 125, 462
BslFI GGGAC 1 cut(s) 711
BslI CCNNNNNNNGG 7 cut(s) 125, 166, 467, 853, 1039, 1040, 1064
BsmAI GTCTC 2 cut(s) 787, 962
BsmFI GGGAC 1 cut(s) 711
BsmI GAATGC 4 cut(s) 37, 211, 231, 834
BsnI GGCC 3 cut(s) 136, 331, 1057
Bso31I GGTCTC 1 cut(s) 962
BsoBI CYCGRG 1 cut(s) 606
Bsp119I TTCGAA 1 cut(s) 575
Bsp1286I GDGCHC 3 cut(s) 207, 613, 988
Bsp143I GATC 6 cut(s) 424, 543, 554, 725, 752, 1082
Bsp1720I GCTNAGC 1 cut(s) 201
Bsp19I CCATGG 1 cut(s) 195
Bsp68I TCGCGA 1 cut(s) 95
BspACI CCGC 9 cut(s) 113, 116, 260, 284, 666, 771, 816, 1050, 1131
BspANI GGCC 3 cut(s) 136, 331, 1057
BspCNI CTCAG 6 cut(s) 55, 178, 193, 1051, 1082, 1088
BspFNI CGCG 3 cut(s) 95, 110, 1131
BspLI GGNNCC 1 cut(s) 754
BspMAI CTGCAG 2 cut(s) 279, 1000
BspOI GCTAGC 1 cut(s) 174
BspPI GGATC 5 cut(s) 419, 562, 733, 747, 760
BspT104I TTCGAA 1 cut(s) 575
BspTNI GGTCTC 1 cut(s) 962
BsrBI CCGCTC 1 cut(s) 1050
BsrI ACTGG 2 cut(s) 472, 637
BssECI CCNNGG 3 cut(s) 87, 195, 605
BssMI GATC 6 cut(s) 424, 543, 554, 725, 752, 1082
BssT1I CCWWGG 1 cut(s) 195
Bst2UI CCWGG 4 cut(s) 30, 89, 180, 978
Bst4CI ACNGT 2 cut(s) 294, 640
Bst6I CTCTTC 1 cut(s) 415
BstBI TTCGAA 1 cut(s) 575
BstC8I GCNNGC 3 cut(s) 35, 172, 844
BstDEI CTNAG 9 cut(s) 42, 165, 201, 402, 713, 939, 1038, 1069, 1075
BstDSI CCRYGG 1 cut(s) 195
BstENI CCTNNNNNAGG 1 cut(s) 851
BstF5I GGATG 4 cut(s) 10, 249, 510, 1058
BstFNI CGCG 3 cut(s) 95, 110, 1131
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 6 cut(s) 427, 546, 557, 728, 755, 1085
BstMAI GTCTC 2 cut(s) 787, 962
BstMBI GATC 6 cut(s) 424, 543, 554, 725, 752, 1082
BstMWI GCNNNNNNNGC 5 cut(s) 254, 274, 283, 524, 992
BstNI CCWGG 4 cut(s) 30, 89, 180, 978
BstSCI CCNGG 6 cut(s) 28, 87, 124, 178, 460, 976
BstSFI CTRYAG 2 cut(s) 275, 996
BstSLI GKGCMC 1 cut(s) 988
BstUI CGCG 3 cut(s) 95, 110, 1131
BstV1I GCAGC 7 cut(s) 244, 267, 277, 289, 851, 982, 1010
BstV2I GAAGAC 1 cut(s) 95
BstX2I RGATCY 2 cut(s) 725, 752
BstYI RGATCY 2 cut(s) 725, 752
Bsu36I CCTNAGG 1 cut(s) 165
BsuRI GGCC 3 cut(s) 136, 331, 1057
BtgI CCRYGG 1 cut(s) 195
BtsCI GGATG 4 cut(s) 10, 249, 510, 1058
BtsIMutI CAGTG 3 cut(s) 553, 630, 1110
BtuMI TCGCGA 1 cut(s) 95
Cac8I GCNNGC 3 cut(s) 35, 172, 844
CaiI CAGNNNCTG 2 cut(s) 632, 995
CfoI GCGC 1 cut(s) 112
Cfr13I GGNCC 3 cut(s) 182, 794, 1056
CsiI ACCWGGT 1 cut(s) 976
Csp6I GTAC 1 cut(s) 877
CviAII CATG 4 cut(s) 196, 238, 539, 806
CviQI GTAC 1 cut(s) 877
DdeI CTNAG 9 cut(s) 42, 165, 201, 402, 713, 939, 1038, 1069, 1075
DpnI GATC 6 cut(s) 426, 545, 556, 727, 754, 1084
DpnII GATC 6 cut(s) 424, 543, 554, 725, 752, 1082
Eam1104I CTCTTC 1 cut(s) 415
EarI CTCTTC 1 cut(s) 415
Eco130I CCWWGG 1 cut(s) 195
Eco24I GRGCYC 1 cut(s) 613
Eco31I GGTCTC 1 cut(s) 962
Eco47I GGWCC 2 cut(s) 182, 794
Eco57I CTGAAG 3 cut(s) 38, 630, 819
Eco81I CCTNAGG 1 cut(s) 165
Eco88I CYCGRG 1 cut(s) 606
EcoNI CCTNNNNNAGG 1 cut(s) 851
EcoO109I RGGNCCY 1 cut(s) 182
EcoRII CCWGG 4 cut(s) 28, 87, 178, 976
EcoT14I CCWWGG 1 cut(s) 195
EcoT38I GRGCYC 1 cut(s) 613
ErhI CCWWGG 1 cut(s) 195
FaeI CATG 4 cut(s) 199, 241, 542, 809
FaiI YATR 8 cut(s) 20, 197, 239, 342, 540, 620, 807, 1017
FalI AAGNNNNNCTT 2 cut(s) 467, 499
FaqI GGGAC 1 cut(s) 711
FatI CATG 4 cut(s) 195, 237, 538, 805
FauI CCCGC 2 cut(s) 673, 1124
FbaI TGATCA 1 cut(s) 543
FblI GTMKAC 1 cut(s) 454
FokI GGATG 4 cut(s) 17, 256, 517, 1065
FriOI GRGCYC 1 cut(s) 613
FspBI CTAG 2 cut(s) 171, 1095
GlaI GCGC 1 cut(s) 111
GsaI CCCAGC 1 cut(s) 1037
HaeIII GGCC 3 cut(s) 136, 331, 1057
HapII CCGG 2 cut(s) 125, 462
HhaI GCGC 1 cut(s) 112
Hin1II CATG 4 cut(s) 199, 241, 542, 809
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HincII GTYRAC 1 cut(s) 455
HindII GTYRAC 1 cut(s) 455
HinfI GANTC 4 cut(s) 407, 709, 1091, 1126
HpaII CCGG 2 cut(s) 125, 462
HphI GGTGA 3 cut(s) 149, 509, 748
Hpy166II GTNNAC 4 cut(s) 455, 662, 877, 986
Hpy188I TCNGA 5 cut(s) 57, 217, 224, 298, 424
Hpy188III TCNNGA 4 cut(s) 94, 347, 558, 797
Hpy8I GTNNAC 4 cut(s) 455, 662, 877, 986
Hpy99I CGWCG 2 cut(s) 298, 1009
HpyAV CCTTC 6 cut(s) 93, 321, 465, 690, 740, 794
HpyCH4III ACNGT 2 cut(s) 294, 640
HpyCH4IV ACGT 2 cut(s) 322, 700
HpyCH4V TGCA 7 cut(s) 265, 277, 434, 533, 890, 986, 998
HpyF10VI GCNNNNNNNGC 5 cut(s) 254, 274, 283, 524, 992
HpyF3I CTNAG 9 cut(s) 42, 165, 201, 402, 713, 939, 1038, 1069, 1075
HpySE526I ACGT 2 cut(s) 322, 700
Hsp92II CATG 4 cut(s) 199, 241, 542, 809
HspAI GCGC 1 cut(s) 110
Ksp22I TGATCA 1 cut(s) 543
Kzo9I GATC 6 cut(s) 424, 543, 554, 725, 752, 1082
LmnI GCTCC 2 cut(s) 298, 881
Lsp1109I GCAGC 7 cut(s) 244, 267, 277, 289, 851, 982, 1010
LweI GCATC 1 cut(s) 1010
MabI ACCWGGT 1 cut(s) 976
MaeI CTAG 2 cut(s) 171, 1095
MaeII ACGT 2 cut(s) 322, 700
MaeIII GTNAC 1 cut(s) 649
MalI GATC 6 cut(s) 426, 545, 556, 727, 754, 1084
MbiI CCGCTC 1 cut(s) 1050
MboI GATC 6 cut(s) 424, 543, 554, 725, 752, 1082
MboII GAAGA 8 cut(s) 44, 95, 328, 402, 405, 575, 636, 755
MflI RGATCY 2 cut(s) 725, 752
MhlI GDGCHC 3 cut(s) 207, 613, 988
MluCI AATT 1 cut(s) 822
MlyI GAGTC 3 cut(s) 401, 1085, 1120
MmeI TCCRAC 2 cut(s) 276, 532
MroXI GAANNNNTTC 1 cut(s) 640
MseI TTAA 2 cut(s) 312, 686
MslI CAYNNNNRTG 2 cut(s) 236, 242
MspA1I CMGCKG 2 cut(s) 280, 518
MspI CCGG 2 cut(s) 125, 462
MspR9I CCNGG 6 cut(s) 30, 89, 126, 180, 462, 978
Mva1269I GAATGC 4 cut(s) 37, 211, 231, 834
MvaI CCWGG 4 cut(s) 30, 89, 180, 978
MvnI CGCG 3 cut(s) 95, 110, 1131
MwoI GCNNNNNNNGC 5 cut(s) 254, 274, 283, 524, 992
NciI CCSGG 2 cut(s) 126, 462
NcoI CCATGG 1 cut(s) 195
NdeII GATC 6 cut(s) 424, 543, 554, 725, 752, 1082
NheI GCTAGC 1 cut(s) 170
NlaIII CATG 4 cut(s) 199, 241, 542, 809
NlaIV GGNNCC 1 cut(s) 754
NmuCI GTSAC 1 cut(s) 649
NruI TCGCGA 1 cut(s) 95
NspV TTCGAA 1 cut(s) 575
PcsI WCGNNNNNNNCGW 1 cut(s) 302
PctI GAATGC 4 cut(s) 37, 211, 231, 834
PdmI GAANNNNTTC 1 cut(s) 640
PfeI GAWTC 1 cut(s) 709
PfoI TCCNGGA 3 cut(s) 124, 178, 460
PleI GAGTC 3 cut(s) 401, 1085, 1120
PpsI GAGTC 3 cut(s) 401, 1085, 1120
PpuMI RGGWCCY 1 cut(s) 182
Psp5II RGGWCCY 1 cut(s) 182
Psp6I CCWGG 4 cut(s) 28, 87, 178, 976
PspFI CCCAGC 1 cut(s) 1033
PspGI CCWGG 4 cut(s) 28, 87, 178, 976
PspN4I GGNNCC 1 cut(s) 754
PspPI GGNCC 3 cut(s) 182, 794, 1056
PspPPI RGGWCCY 1 cut(s) 182
PstI CTGCAG 2 cut(s) 279, 1000
PstNI CAGNNNCTG 2 cut(s) 632, 995
PsuI RGATCY 2 cut(s) 725, 752
PvuII CAGCTG 2 cut(s) 280, 518
RruI TCGCGA 1 cut(s) 95
RsaI GTAC 1 cut(s) 878
RsaNI GTAC 1 cut(s) 877
RseI CAYNNNNRTG 2 cut(s) 236, 242
SalI GTCGAC 1 cut(s) 453
SaqAI TTAA 2 cut(s) 312, 686
Sau3AI GATC 6 cut(s) 424, 543, 554, 725, 752, 1082
Sau96I GGNCC 3 cut(s) 182, 794, 1056
SchI GAGTC 3 cut(s) 401, 1085, 1120
ScrFI CCNGG 6 cut(s) 30, 89, 126, 180, 462, 978
SduI GDGCHC 3 cut(s) 207, 613, 988
SexAI ACCWGGT 1 cut(s) 976
SfaNI GCATC 1 cut(s) 1010
SfcI CTRYAG 2 cut(s) 275, 996
SfuI TTCGAA 1 cut(s) 575
SinI GGWCC 2 cut(s) 182, 794
SmiMI CAYNNNNRTG 2 cut(s) 236, 242
Sse9I AATT 1 cut(s) 822
SsiI CCGC 9 cut(s) 113, 116, 260, 284, 666, 771, 816, 1050, 1131
SspMI CTAG 2 cut(s) 171, 1095
StyD4I CCNGG 6 cut(s) 28, 87, 124, 178, 460, 976
StyI CCWWGG 1 cut(s) 195
TaaI ACNGT 2 cut(s) 294, 640
TaiI ACGT 2 cut(s) 325, 703
TaqI TCGA 4 cut(s) 454, 575, 763, 1004
TasI AATT 1 cut(s) 822
TauI GCSGC 3 cut(s) 115, 118, 286
TfiI GAWTC 1 cut(s) 709
Tru1I TTAA 2 cut(s) 312, 686
Tru9I TTAA 2 cut(s) 312, 686
TscAI CASTG 3 cut(s) 553, 637, 1117
TseFI GTSAC 1 cut(s) 649
TseI GCWGC 7 cut(s) 257, 265, 277, 280, 839, 995, 998
Tsp45I GTSAC 1 cut(s) 649
TspDTI ATGAA 3 cut(s) 351, 521, 756
TspGWI ACGGA 1 cut(s) 894
TspRI CASTG 3 cut(s) 553, 637, 1117
VneI GTGCAC 1 cut(s) 984
VpaK11BI GGWCC 2 cut(s) 182, 794
XagI CCTNNNNNAGG 1 cut(s) 851
XcmI CCANNNNNNNNNTGG 2 cut(s) 95, 961
XmiI GTMKAC 1 cut(s) 454
XmnI GAANNNNTTC 1 cut(s) 640
XspI CTAG 2 cut(s) 171, 1095
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.