RLG00000003902

DNA-binding protein ESCAROLA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0005616
Physical Location & Seq
Reverse (-)
54086276 .. 54090294
4019 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003902

Sequence Viewer

Length: 1020 bp
ATGGATGGGAGAGAAGCCATGGCCATCTCTGGTGCTCAAGCTTCATACTACATCCATAGGGGAGGCCATGGTGGGTCTTCACCTGGATCACAGATTGCTGGGCTGCATGCACCACCTGGGTTCAGGCCCAACAACACTGCCTTTCAACAAGCTCAGTCTAATGTGAGGGCCACCTCAGCATTCTCAGTAGAGCCTTCTTCAAGACCCAATTACCCTCATGGCATTAGCATCAATGTTTCTCCCGGAGGAGCTCCTTCTTCCGGTGAGCCTGTAAAGAAGAAACGAGGGAGGCCCCGGAAGTATGGGCCTGGTCCTGCTCCTGGTCCCGCTCCTGATGCCCCGGTTTCTCTGGCATTGTCTCCAATGTCTTCAACTCCTAATCCTACTCCAGGATCAACCTCACCGACTCCGAAACGAAACAGAGGAAGGCCACCTGGGACTGGCAGGAAACAACAATTGGCTAATCTTGCCGAAACATCTACCAAACATATATTAGCTGACATTGCAGCAAAATTATTGTTATTTTCACAACAGAGGCCAAGGGCACTATGCATCTTGTCAGGAAATGGAACAGTATCTTCAGTAGCACTGCGTCAGCCTGCATCAACTGGTGTCAGTGTCACATATGAGGGTCGTTTCCAGATATTATGCTTGTCAGGTTCTTACCTGGTTGCTGAGGATGGTGGCCCCCGCGATCGAACAGGGGGGATAAGCGTTTCCCTTTCTAGTTCTGATGGTCATGTCATTGGTGGTGCTGTGGCTATGCTTATTGCAGCAACTCCAGTGCAGATTGTGCTTTGCAGTTTTGTATATGGTAGCTCCAACAAGATCAAGAGCAAGCCAGTGGCTGACCCCAATAATGGGCAGAGTTCTGAGCCTCAGCACAGTGAGAAATTACCTTCACCGGCTACAGCTCCATCTACTCAAAGTTATGCTCCATCCACAGCAGGCATTTGGCCTGGGGGGCGGCTAGTTGATGCGAGAAATGCTCACACCGGTATTGACTTGACTCGCGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0043621
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

340

Amino Acids

34.79

Weight (kDa)

10.17

Isoelectric Point (pI)

51.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 167 - 269 1.9e-17 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 329
AccII CGCG 2 cut(s) 693, 1014
AciI CCGC 4 cut(s) 327, 691, 967, 1014
AclWI GGATC 2 cut(s) 94, 400
AcoI YGGCCR 1 cut(s) 21
AcuI CTGAAG 1 cut(s) 564
AfiI CCNNNNNNNGG 6 cut(s) 260, 320, 389, 440, 860, 861
AgeI ACCGGT 1 cut(s) 995
AgsI TTSAA 3 cut(s) 146, 201, 372
AjnI CCWGG 8 cut(s) 82, 115, 307, 319, 388, 433, 666, 958
AjuI GAANNNNNNNTTGG 2 cut(s) 440, 472
AluBI AGCT 6 cut(s) 41, 152, 251, 497, 819, 914
AluI AGCT 6 cut(s) 41, 152, 251, 497, 819, 914
Alw21I GWGCWC 2 cut(s) 37, 253
Alw26I GTCTC 1 cut(s) 363
AlwI GGATC 2 cut(s) 94, 400
AlwNI CAGNNNCTG 1 cut(s) 848
ApeKI GCWGC 3 cut(s) 103, 506, 773
AsiGI ACCGGT 1 cut(s) 995
AspS9I GGNCC 7 cut(s) 126, 168, 291, 305, 311, 323, 686
AsuC2I CCSGG 3 cut(s) 243, 295, 341
AsuHPI GGTGA 4 cut(s) 72, 275, 393, 894
AvaII GGWCC 2 cut(s) 311, 323
BaeGI GKGCMC 1 cut(s) 547
BalI TGGCCA 1 cut(s) 23
BanII GRGCYC 1 cut(s) 253
BbsI GAAGAC 2 cut(s) 69, 360
Bbv12I GWGCWC 2 cut(s) 37, 253
BbvCI CCTCAGC 3 cut(s) 175, 675, 879
BbvI GCAGC 3 cut(s) 90, 518, 785
BccI CCATC 5 cut(s) 32, 674, 728, 925, 946
BciT130I CCWGG 8 cut(s) 84, 117, 309, 321, 390, 435, 668, 960
BcnI CCSGG 3 cut(s) 243, 295, 341
BcoDI GTCTC 1 cut(s) 363
BfaI CTAG 2 cut(s) 726, 971
BfmI CTRYAG 1 cut(s) 909
BglI GCCNNNNNGGC 1 cut(s) 964
BisI GCNGC 4 cut(s) 104, 507, 774, 968
BlsI GCNGC 4 cut(s) 105, 508, 775, 969
Bme18I GGWCC 2 cut(s) 311, 323
BmgT120I GGNCC 7 cut(s) 126, 168, 291, 305, 311, 323, 686
BmiI GGNNCC 3 cut(s) 293, 325, 688
BmsI GCATC 5 cut(s) 237, 325, 561, 611, 967
BpiI GAAGAC 2 cut(s) 69, 360
BplI GAGNNNNNCTC 2 cut(s) 973, 1005
BpmI CTGGAG 2 cut(s) 372, 765
Bpu10I CCTNAGC 3 cut(s) 175, 675, 879
BpuEI CTTGAG 1 cut(s) 21
BpuMI CCSGG 3 cut(s) 243, 295, 341
BsaJI CCNNGG 8 cut(s) 18, 67, 116, 293, 339, 434, 539, 959
BsaWI WCCGGW 2 cut(s) 260, 995
Bsc4I CCNNNNNNNGG 6 cut(s) 260, 320, 389, 440, 860, 861
Bse118I RCCGGY 2 cut(s) 904, 995
Bse1I ACTGG 4 cut(s) 445, 613, 782, 842
Bse3DI GCAATG 1 cut(s) 501
BseBI CCWGG 8 cut(s) 84, 117, 309, 321, 390, 435, 668, 960
BseDI CCNNGG 8 cut(s) 18, 67, 116, 293, 339, 434, 539, 959
BseGI GGATG 4 cut(s) 10, 51, 685, 938
BseLI CCNNNNNNNGG 6 cut(s) 260, 320, 389, 440, 860, 861
BseMI GCAATG 1 cut(s) 501
BseMII CTCAG 6 cut(s) 167, 189, 198, 666, 864, 893
BseNI ACTGG 4 cut(s) 445, 613, 782, 842
BseRI GAGGAG 1 cut(s) 261
BseSI GKGCMC 1 cut(s) 547
BseXI GCAGC 3 cut(s) 90, 518, 785
BseYI CCCAGC 1 cut(s) 98
BsgI GTGCAG 1 cut(s) 806
Bsh1236I CGCG 2 cut(s) 693, 1014
Bsh1285I CGRYCG 1 cut(s) 697
BshTI ACCGGT 1 cut(s) 995
BsiEI CGRYCG 1 cut(s) 697
BsiHKAI GWGCWC 2 cut(s) 37, 253
BsiSI CCGG 6 cut(s) 243, 261, 295, 341, 905, 996
BslFI GGGAC 2 cut(s) 309, 451
BslI CCNNNNNNNGG 6 cut(s) 260, 320, 389, 440, 860, 861
BsmAI GTCTC 1 cut(s) 363
BsmFI GGGAC 2 cut(s) 309, 451
BsmI GAATGC 1 cut(s) 179
Bsp1286I GDGCHC 3 cut(s) 37, 253, 547
Bsp143I GATC 4 cut(s) 86, 392, 694, 828
Bsp19I CCATGG 2 cut(s) 18, 67
BspACI CCGC 4 cut(s) 327, 691, 967, 1014
BspCNI CTCAG 6 cut(s) 166, 188, 197, 667, 865, 892
BspFNI CGCG 2 cut(s) 693, 1014
BspLI GGNNCC 3 cut(s) 293, 325, 688
BspPI GGATC 2 cut(s) 94, 400
BsrBI CCGCTC 1 cut(s) 329
BsrDI GCAATG 1 cut(s) 501
BsrFI RCCGGY 2 cut(s) 904, 995
BsrI ACTGG 4 cut(s) 445, 613, 782, 842
BssAI RCCGGY 2 cut(s) 904, 995
BssECI CCNNGG 8 cut(s) 18, 67, 116, 293, 339, 434, 539, 959
BssMI GATC 4 cut(s) 86, 392, 694, 828
BssT1I CCWWGG 3 cut(s) 18, 67, 539
Bst2UI CCWGG 8 cut(s) 84, 117, 309, 321, 390, 435, 668, 960
Bst4CI ACNGT 2 cut(s) 574, 887
BstAPI GCANNNNNTGC 1 cut(s) 793
BstC8I GCNNGC 4 cut(s) 108, 600, 839, 949
BstDEI CTNAG 6 cut(s) 153, 175, 184, 675, 873, 879
BstDSI CCRYGG 2 cut(s) 18, 67
BstENI CCTNNNNNAGG 1 cut(s) 387
BstF5I GGATG 4 cut(s) 10, 51, 685, 938
BstFNI CGCG 2 cut(s) 693, 1014
BstKTI GATC 4 cut(s) 89, 395, 697, 831
BstMAI GTCTC 1 cut(s) 363
BstMBI GATC 4 cut(s) 86, 392, 694, 828
BstMCI CGRYCG 1 cut(s) 697
BstMWI GCNNNNNNNGC 7 cut(s) 176, 335, 467, 503, 793, 964, 986
BstNI CCWGG 8 cut(s) 84, 117, 309, 321, 390, 435, 668, 960
BstNSI RCATGY 1 cut(s) 110
BstSFI CTRYAG 1 cut(s) 909
BstSLI GKGCMC 1 cut(s) 547
BstUI CGCG 2 cut(s) 693, 1014
BstV1I GCAGC 3 cut(s) 90, 518, 785
BstV2I GAAGAC 2 cut(s) 69, 360
BtgI CCRYGG 2 cut(s) 18, 67
BtsCI GGATG 4 cut(s) 10, 51, 685, 938
BtsI GCAGTG 2 cut(s) 135, 587
BtsIMutI CAGTG 6 cut(s) 135, 587, 622, 789, 849, 892
Cac8I GCNNGC 4 cut(s) 108, 600, 839, 949
CaiI CAGNNNCTG 1 cut(s) 848
Cfr10I RCCGGY 2 cut(s) 904, 995
Cfr13I GGNCC 7 cut(s) 126, 168, 291, 305, 311, 323, 686
CseI GACGC 1 cut(s) 581
CsiI ACCWGGT 1 cut(s) 666
CspAI ACCGGT 1 cut(s) 995
CviAII CATG 5 cut(s) 19, 68, 107, 218, 740
DdeI CTNAG 6 cut(s) 153, 175, 184, 675, 873, 879
DpnI GATC 4 cut(s) 88, 394, 696, 830
DpnII GATC 4 cut(s) 86, 392, 694, 828
EaeI YGGCCR 1 cut(s) 21
Ecl136II GAGCTC 1 cut(s) 251
Eco130I CCWWGG 3 cut(s) 18, 67, 539
Eco24I GRGCYC 1 cut(s) 253
Eco47I GGWCC 2 cut(s) 311, 323
Eco53kI GAGCTC 1 cut(s) 251
Eco57I CTGAAG 1 cut(s) 564
EcoICRI GAGCTC 1 cut(s) 251
EcoNI CCTNNNNNAGG 1 cut(s) 387
EcoO109I RGGNCCY 1 cut(s) 291
EcoRII CCWGG 8 cut(s) 82, 115, 307, 319, 388, 433, 666, 958
EcoT14I CCWWGG 3 cut(s) 18, 67, 539
EcoT22I ATGCAT 1 cut(s) 554
EcoT38I GRGCYC 1 cut(s) 253
ErhI CCWWGG 3 cut(s) 18, 67, 539
FaeI CATG 5 cut(s) 22, 71, 110, 221, 743
FaqI GGGAC 2 cut(s) 309, 451
FatI CATG 5 cut(s) 18, 67, 106, 217, 739
FauI CCCGC 2 cut(s) 334, 698
FauNDI CATATG 1 cut(s) 625
Fnu4HI GCNGC 4 cut(s) 104, 507, 774, 968
FokI GGATG 4 cut(s) 17, 38, 692, 925
FriOI GRGCYC 1 cut(s) 253
Fsp4HI GCNGC 4 cut(s) 104, 507, 774, 968
FspBI CTAG 2 cut(s) 726, 971
GluI GCNGC 4 cut(s) 104, 507, 774, 968
GsaI CCCAGC 1 cut(s) 102
GsuI CTGGAG 2 cut(s) 372, 765
HapII CCGG 6 cut(s) 243, 261, 295, 341, 905, 996
HgaI GACGC 1 cut(s) 581
Hin1II CATG 5 cut(s) 22, 71, 110, 221, 743
HindIII AAGCTT 1 cut(s) 39
HinfI GANTC 2 cut(s) 406, 1009
HpaII CCGG 6 cut(s) 243, 261, 295, 341, 905, 996
HphI GGTGA 4 cut(s) 72, 275, 393, 894
Hpy188I TCNGA 3 cut(s) 411, 733, 874
Hpy188III TCNNGA 5 cut(s) 201, 332, 561, 640, 832
HpyAV CCTTC 4 cut(s) 204, 264, 420, 909
HpyCH4III ACNGT 2 cut(s) 574, 887
HpyCH4V TGCA 8 cut(s) 106, 110, 506, 552, 602, 773, 787, 801
HpyF10VI GCNNNNNNNGC 7 cut(s) 176, 335, 467, 503, 793, 964, 986
HpyF3I CTNAG 6 cut(s) 153, 175, 184, 675, 873, 879
Hsp92II CATG 5 cut(s) 22, 71, 110, 221, 743
Kzo9I GATC 4 cut(s) 86, 392, 694, 828
LmnI GCTCC 7 cut(s) 248, 256, 322, 334, 824, 919, 940
Lsp1109I GCAGC 3 cut(s) 90, 518, 785
LweI GCATC 5 cut(s) 237, 325, 561, 611, 967
MabI ACCWGGT 1 cut(s) 666
MaeI CTAG 2 cut(s) 726, 971
MaeIII GTNAC 1 cut(s) 619
MalI GATC 4 cut(s) 88, 394, 696, 830
MbiI CCGCTC 1 cut(s) 329
MboI GATC 4 cut(s) 86, 392, 694, 828
MboII GAAGA 6 cut(s) 69, 189, 249, 289, 360, 570
MfeI CAATTG 1 cut(s) 455
MhlI GDGCHC 3 cut(s) 37, 253, 547
MlsI TGGCCA 1 cut(s) 23
MluCI AATT 4 cut(s) 208, 455, 512, 893
MluNI TGGCCA 1 cut(s) 23
MlyI GAGTC 2 cut(s) 400, 1003
MmeI TCCRAC 1 cut(s) 846
Mox20I TGGCCA 1 cut(s) 23
Mph1103I ATGCAT 1 cut(s) 554
MscI TGGCCA 1 cut(s) 23
Msp20I TGGCCA 1 cut(s) 23
MspI CCGG 6 cut(s) 243, 261, 295, 341, 905, 996
MunI CAATTG 1 cut(s) 455
Mva1269I GAATGC 1 cut(s) 179
MvaI CCWGG 8 cut(s) 84, 117, 309, 321, 390, 435, 668, 960
MvnI CGCG 2 cut(s) 693, 1014
MwoI GCNNNNNNNGC 7 cut(s) 176, 335, 467, 503, 793, 964, 986
NciI CCSGG 3 cut(s) 243, 295, 341
NcoI CCATGG 2 cut(s) 18, 67
NdeI CATATG 1 cut(s) 625
NdeII GATC 4 cut(s) 86, 392, 694, 828
NlaIII CATG 5 cut(s) 22, 71, 110, 221, 743
NlaIV GGNNCC 3 cut(s) 293, 325, 688
NmuCI GTSAC 1 cut(s) 619
NsiI ATGCAT 1 cut(s) 554
NspI RCATGY 1 cut(s) 110
PaeI GCATGC 1 cut(s) 110
PctI GAATGC 1 cut(s) 179
PfoI TCCNGGA 2 cut(s) 241, 388
PinAI ACCGGT 1 cut(s) 995
PkrI GCNGC 4 cut(s) 105, 508, 775, 969
Ple19I CGATCG 1 cut(s) 697
PleI GAGTC 2 cut(s) 400, 1003
PpsI GAGTC 2 cut(s) 400, 1003
Psp124BI GAGCTC 1 cut(s) 253
Psp6I CCWGG 8 cut(s) 82, 115, 307, 319, 388, 433, 666, 958
PspFI CCCAGC 1 cut(s) 98
PspGI CCWGG 8 cut(s) 82, 115, 307, 319, 388, 433, 666, 958
PspN4I GGNNCC 3 cut(s) 293, 325, 688
PspPI GGNCC 7 cut(s) 126, 168, 291, 305, 311, 323, 686
PstNI CAGNNNCTG 1 cut(s) 848
PvuI CGATCG 1 cut(s) 697
SacI GAGCTC 1 cut(s) 253
SatI GCNGC 4 cut(s) 104, 507, 774, 968
Sau3AI GATC 4 cut(s) 86, 392, 694, 828
Sau96I GGNCC 7 cut(s) 126, 168, 291, 305, 311, 323, 686
SchI GAGTC 2 cut(s) 400, 1003
SduI GDGCHC 3 cut(s) 37, 253, 547
SexAI ACCWGGT 1 cut(s) 666
SfaNI GCATC 5 cut(s) 237, 325, 561, 611, 967
SfcI CTRYAG 1 cut(s) 909
SinI GGWCC 2 cut(s) 311, 323
SmlI CTYRAG 1 cut(s) 36
SmoI CTYRAG 1 cut(s) 36
SphI GCATGC 1 cut(s) 110
Sse9I AATT 4 cut(s) 208, 455, 512, 893
SsiI CCGC 4 cut(s) 327, 691, 967, 1014
SspMI CTAG 2 cut(s) 726, 971
SstI GAGCTC 1 cut(s) 253
StyI CCWWGG 3 cut(s) 18, 67, 539
TaaI ACNGT 2 cut(s) 574, 887
TaqI TCGA 1 cut(s) 697
TasI AATT 4 cut(s) 208, 455, 512, 893
TauI GCSGC 1 cut(s) 970
TscAI CASTG 6 cut(s) 142, 594, 622, 789, 849, 892
TseFI GTSAC 1 cut(s) 619
TseI GCWGC 3 cut(s) 103, 506, 773
Tsp45I GTSAC 1 cut(s) 619
TspDTI ATGAA 1 cut(s) 33
TspRI CASTG 6 cut(s) 142, 594, 622, 789, 849, 892
VpaK11BI GGWCC 2 cut(s) 311, 323
XagI CCTNNNNNAGG 1 cut(s) 387
XceI RCATGY 1 cut(s) 110
XspI CTAG 2 cut(s) 726, 971
Zsp2I ATGCAT 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.