Rorug07G0044600

DNA-binding protein ESCAROLA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0005616
Physical Location & Seq
Reverse (-)
3258869 .. 3264600
5732 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0044600.1

Sequence Viewer

Length: 5655 bp
ATGGCTGCATGTCCCTGGGCATTCTTGGGAGGCCTGGACACGGCCTCTGCGTCTCCTACAACAGCCCCAAAATCACGCACTTTTGCATCTATTCTCGCCAACTCAACAGAGGCTGCTATATCTCTCTGCCAACTACCATCCCCAGTTGTTCGCGGGGATAAAATTTATGTCAAGATCAATGAATCCTTATATCAGGAGCAATTGAAGCAATTCAAAACCAATCTTATTGGTCGTCTTCTTCTACGTAAGGGTTCTAGACCTCTTAAGGCTGATGCCCTAAAAGGATTTCTAGCTGCTCTCTGGCAGCCTTCAAAGCCATGGCGTCTTGTGCCTCTTGGTAAAGGCTACTTTGACATTCATTTTGCCACAGAAGAGGATCTGCGTAGGGTATGGAGTGGAGGTACTTGTACTCTTCCGGATGGAATTTTTCGCCTAACTCAGTGGAAACCAGATTTTGTACCTGGAGTTTCCTTTCCACAAACTCATGCTCAAATTTGGGTTAGGTTTTATGGTCTGAGCCAAGATTATTGGCACCAACAACATCTTATGGAGATTGCTCGGGGGGTCGGAACTCCTTTGCAAATTGATAAAGCCACAAGGGAAAGACAATTTGGCTACTTTGCTAGAGTCTTAATTGATGTGGATCTTGCTGGTGAACTACCACCTTCTTTGATGGTTGAGCGTGAAACTCATTGTTTTCAAATTGAAGTAGTCTATGAGAATGTGTGTACTCATTGTGGGAGGGTTGGCCACATGGCTGACCAATGCAGGGCCTTGAAGAGCTCTAACAAAGAGCAAAATGCTCAAGTACAAAAGGCAGTTAAAAAGCCTTCTCTCATTGGGCGTCAAGAGTATCGTCCCAGGACGGCTGTAAACAATGTTTCTGAGCAAAACTTGGACACTACTCCTCAGGTGCAACTCTCCCCTGATGTTGCAAACCATGAACAGATTACTAAATTTGCAGAAGATGTTCTCACTGAGGCCATTGACCAGGAATTGGATCGTATTGCAGATTTGGTCATTATTGAACCCATAACCAACGGGAAGGCTGGATGTTGTAGAGGCAACATAACTCCTCTTATCAATATGAATAATTCTGTCATCCTTGCAAATAAGGAAACTGAAGCAGGGGTTGACACTACTCCTAATGATTTTGTTGTAGTTGTTGGTCCAACTATTTTGGAGGACCCTTCGGTCAATATTTCTGATGAGGATGATGAAGTTGAGATGTGTGATGATGATGTGAGCTCCAACATGGAGCTAAATGCTTATAGTACTGCCCAGCCAACCAACCCCCTTTCACATAGTGGTCAGAGTTGGCATGATATGGTGGAAGAGGAAAATATTATTGATCTGGCAGATTTAGCTACGGGTCTTGCTCCCCCGGGATTCGAACATGTTGCAACTTTAGCCAAGGATGTCTCATTAATTACTGGTCATGGGGATAACGGAGATACAGAGAATGGTTTCACACCGGTTCTCTCTAAGTCTCAACAGAAGAAACAAAGACGACAAGCTACTCTAGCTCTTGCTCGTGAAAACCCTTATCCAAAGAGGGACAGAACAAAGAATAAAAAGGGAATAGGAAACAATGACTCTCAATCCGAGCTTTCCAATATTTGCCGTATTCACAAACCAGATCTTGTTTGCATTGCTGAGCCAAAGGTAAAGTTCACCTCAATTTCAGCAGTTTTTTGGTCTTCTTTGAATTTGGATCTTGTTGCAACTAATGATTGTGGTGCGGCTCTTCCAAATCTTTGGTTGTTGAAGAGTTTTCATATTCCTAATCCGACGATTATTTCCAACTCCGAGCAGCAGGTTACAATTCAGGTTCCTCTTGATAATGAGCCCAGTCAACTTTCTTTTGTATATGCTTCTACCTCTTATATCAAACGCAGGGATTTATGGCAACATCTTTATGCTCTTCGTCCTCAAACGTCTATCCCATGGATGGTAATTGGTGACTTCAACGCTGTGTTAGGAGCCCACGAGAAATCGGGAGGCCCATCTCCTTCTCGCCTCTCTTGTGCAGATTTTCAAAGTTTGTTCTTTACTTGGTCAAATGGTTGGAGCACCCGTGGCCATGTTGAGCTTCGTCTGGACAGATGTCTCTGTGATACCACTTGGTTTGAAGCTTGGCCTTTTACTAGCTGTGTTGCTCTTCCCCGTGTTGTTTCTGATCATTCTCCACTGATTTTCTCTGCTTCAAAACTCTCCCCAAGTGGTCCTAAGCCTTTCAGGTTTCAGTCTATGTGGTTGCAGCATCCAAATTTTCATGCAGTAGTGTCTAATTGCTGGAGTTCCTTTCAACTTTGTGGCTGCCCAATGTTTGTTGCTTTGCAGAAACTAAAGGCTCTGAAACAGTGTCTGCGTGATTGGAACAAAGCGGTCTTTGGTAATGTCCATCAAAACGTTGCTGCTGCTCGCGAAAATCTAGTCACCATACAACATGATATCGCGACCCGTGGCATGACTGATCAAAAGTTTGAGGATGAGATTGCTGCAAAGTCTTCAGTCTTAAATGCTCTCAGGTTGCAAGAAACCTTTTGGAAAGATAGAGCTCGAGTTAAGTGGCTTACTGATGGGGATCGAAGTACATCTTTTTTCCATACTTATGCCAAGGTTCGTGCAGCAAAGGGTCAAATGTCTTCTATCCGTGTGGGGGAGCGGTTACTTACTGACCCTTCAGACATTGCAGCACATACTGTGGCTTTTTATCAGAATCTCTATGATATTTCCTCCCCTTCTACAAACGTGGATGAGGTTTGTTCTGTTATCCCATCTTTGGTGACAGACGATGAAAATGTGGTGCTATCTGCAATTCCTACATCTGAGGAAATTAGAAACGCAGTTTTTTCTATGGATGGTTCTAGTGCTCCAGGGCCTGATGGTTTTTCAGGGTCTTTTTATCATGCTTGTTGGGATATTGTTGGTACTGATGTGGTGGCATGTGTTCGTCAATTTTTTCTTCAAAACTGGATCCTCCCCAACATGAATTGCAATTTTTTAGTTCTCATTCCAAAAGTCCCAAATGCTCAACTTATTACTCAGTTTCGCCCAATTGCCTTAGCCAATTTTCTCTTCAAGATCATTCCGAAGATTTTGGCTTCTCGCTTGGGGTCTATTGCTGCACGCATAATTTCTCCTCAGCAATCTGCCTTCTTACCTGGTAGACGTATCAATCACTGTATTGGTATGGTTTCGGAATGTTTTAACTTATTGGACCGAAAGGCTTATGGTGGAAATGTGGGAATCAAAGTGGATATAGCAAAGGCATTTGACACTCTGAATTGGGATTTTCTGCTCCGAGTTTTAAGCAATTTTGGGTTCTCTCCTACTTTCACTAGTTGGGTCAGCTCTATTCTTTACTCAGCTAGATTATCTCTTTTAATCAATGGCTCTCCTCAAGGTTTTTTTAAGTGTGCTCGTGGTGTTAGACAAGGAGATCCTCTTTCTCCACTCTTATTCTGCCTTGCAGAGGAGGCTCTCAGCCGGGGTCTTCACCTACTTCTCCAAACCCGTCAATTGAAGCCAATTTCTTTTCCCAGAAATTGCACTGCACCGTCTCATGTGTTATATGCAGATGACCTCATGGTGTTTTGCCGTGGGGACAAAAACTCTCTTAAACGTCTGCGTGGGTTTTTTGACCGTTATAGTGCAGCTTCAGGGCAATTCATTAATGCGGAGAAAAGTACTTTCTATCTTGGAGTTAGGTCTCGTCATAGGAAAGGTACAGTTCAACGTATTTTGGGTTTTCATGCAGGTCGTCTACCTTTTATTTATCTAGGAGTTCCTATATTTTGTGGAAAACCCCGCAGCTGTCATCTCCAAGTTATTGCCGATAGAGCTAAGTCAAGGTTAATGGGTTGGCAGGGGAGGCTTCTCTCTATGGCTGGGCGTACTCAACTTGTGCAATCTGTTTTTCAGAGTATGCTTCTTCATAGTTTTTCAGTGTATCAATGGCCTGCTTACCTTGTGAAGAAGTTATCTACTTGGGCGCGTAATTTTATATGGTCCGGAAATATTGAGACTCGGAAAATTGTCACTGTTGCTTGGACTCAAGTTTGTGCTCCGAAAAAAGAGGGCGGGGTTGGTATTCGTGACCTGGCAACTCTTAATTCTGCGGCTGTTCTAAGGTTTGCATGGAACTCTTTCACTTCTTTGAATCAATGGGGTGATTATATGCGAACTCGTTACCCTATTGTATCTAGTTCCAAAGTGAGTTATCGCAAATCATCAATCTGGCCAGGCTTCCGTTCTATTGCTCTTCATATCACCCACAATTGTCGTTGGATTATTGGAGATGGTCGTTCAGTGTCCTTTTGGAAAGATAAATGGCTTCAGGATCCAATTTTGAAAATTTTGGGTTTTTTCGACTGGTCAGGATTCAGTGATTTACGAGTGGCAGATTTCATCTCAGATCAGTCTTGGCAGTTCCCCTCTTTTTTCCTAGATACCTTTCCAGACTTGTACAGAAAAATTTCTGATATTTGTCTCCCTTTAGACACAGAACCTGATATGCTAATTTGGGAGTCTACAGCCTCTGGAGAGTTATCTTTCACTAACTCTTATAACTTGCTTAGACGTCACTTTATTTTGAGAGACTGGGCTTCTACTATTTGGCATTCCTTCATTCCTCCGCGCTATTCTTTCTTGGCTTGGAGGATTCTCCTTGATCGCTTGCCCACTGATGATCGGTTGAAAAGAGGTGGGATTCCGGTTGTGTCCATCTGCCAATTATGCAACAGTTCGGCAGAGTCAGCGTTGCACCTATTTTTACATTGTCCTTTTTCCCAACATCTTTGGGGATGGCTGGCAACTCAGTTTGGAACCTCACTTCCTGCTTATAGCTCTCTGTTGGATTTTTGGGTTGGCTTTTGTCACAAGGTTTTCTCCCCTCAGCTTTATAATTTGTGGTTGGCAGCTGGCTTGCTTACTTTCATGGAAATTTGGAAGGCGCGAAATAGACTCAGGTTTGATGATCGATCTCCTATCTTTTCTACACTGTGTTGTTCAATTATGGCATGGCTTCGGCAATTTGGTTCCCTTGTTCCTGGTCACTTTAAGGGCGTCCTTGATTCCCGCCTATTATCCTCCCTTGGGGTGTGCCCCAAGCCCCGTAATGCGCCCAAGATTCAACGTGTCATATGGCATCCCCCCCTCTTTCCTTGGGTCAAAGTTAACACTGATGGTCTGGCGAAAGGTAATCCTGGTCCTGCAGCTTGTGGGGGAGTCTTTCGTGATGCCTCTGGGGTTTATTTAGGGAGTTTTTGTCAACCTCTTGGTCGCAATTCATCCTTCTATGCTGAACTTTGTGCGGTTATTGTGTCTATTGAAGTTGCTTTCACCAGAGGTTGGACTACTCTGTGGTTGGAAAGCGATTCTACTAGTGTTTTAGCATCTCTTTCCTCCATTTCTTTCTCCCCTCCATGGGACTTGAGAGTTAGATGGCAAAACTGCCTGAAGAACATTCAACAAATGCAATTTCGGAGCACTCATATTTTTAGGGAAGGGAATGCAGCTGCTGATAAGATGGCAAACTTAGGTGTTTCAAAGCACTCATTTACTTGGTATCCTAGCCCTCCTGCAGAGCTTCACAGGTACTTGCAGGCTGATTTTTTGGGACTCCCAAATTATCGCTTCACAGGTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0043621
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

1884

Amino Acids

211.54

Weight (kDa)

8.88

Isoelectric Point (pI)

42.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 70 - 154 5e-07 Domain of unknown function (DUF4283)
Exo_endo_phos PF03372 531 - 728 6.9e-07 Endonuclease/Exonuclease/phosphatase family
RVT_1 PF00078 1009 - 1265 1.8e-30 Reverse transcriptase (RNA-dependent DNA polymerase)
zf-RVT PF13966 1520 - 1602 3.6e-23 zinc-binding in reverse transcriptase
RVT_3 PF13456 1728 - 1848 6.5e-16 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 4572, 4908
AasI GACNNNNNNGTC 1 cut(s) 1193
AatII GACGTC 1 cut(s) 4588
Acc36I ACCTGC 2 cut(s) 1807, 3752
AccB1I GGYRCC 1 cut(s) 531
AccB7I CCANNNNNTGG 2 cut(s) 997, 5355
AccBSI CCGCTC 1 cut(s) 2668
AccI GTMKAC 3 cut(s) 3172, 3769, 4535
AccII CGCG 6 cut(s) 153, 2427, 2459, 4000, 4642, 4960
AccIII TCCGGA 2 cut(s) 415, 4016
AclI AACGTT 1 cut(s) 2412
AcoI YGGCCR 3 cut(s) 748, 2080, 4244
AcsI RAATTY 9 cut(s) 162, 423, 492, 956, 1708, 2269, 4359, 4479, 4947
AcuI CTGAAG 6 cut(s) 1143, 2496, 2670, 3648, 4325, 5483
AcyI GRCGYC 4 cut(s) 322, 844, 4585, 5070
AdeI CACNNNGTG 1 cut(s) 1307
AflII CTTAAG 1 cut(s) 263
AflIII ACRYGT 2 cut(s) 1396, 5140
AgeI ACCGGT 1 cut(s) 1474
AhlI ACTAGT 2 cut(s) 3344, 5387
AjuI GAANNNNNNNTTGG 4 cut(s) 594, 626, 3065, 3097
AloI GAACNNNNNNTCC 2 cut(s) 4822, 4854
Alw21I GWGCWC 8 cut(s) 785, 1250, 2075, 2563, 2878, 3427, 4072, 5495
Alw26I GTCTC 9 cut(s) 57, 1426, 1494, 2114, 3570, 3720, 4022, 4499, 4596
AlwNI CAGNNNCTG 6 cut(s) 113, 2368, 2885, 4514, 4544, 5525
Ama87I CYCGRG 3 cut(s) 558, 1384, 2562
Aor13HI TCCGGA 2 cut(s) 415, 4016
ApoI RAATTY 9 cut(s) 162, 423, 492, 956, 1708, 2269, 4359, 4479, 4947
ArsI GACNNNNNNTTYG 4 cut(s) 344, 376, 2421, 2453
AseI ATTAAT 2 cut(s) 1427, 3678
AsiGI ACCGGT 1 cut(s) 1474
Asp700I GAANNNNTTC 5 cut(s) 209, 969, 1466, 4151, 4480
AspLEI GCGC 4 cut(s) 4000, 4644, 4960, 5128
AspS9I GGNCC 9 cut(s) 771, 1169, 1186, 2003, 2225, 2882, 3223, 4014, 5213
AsuC2I CCSGG 3 cut(s) 1385, 1386, 3494
AsuHPI GGTGA 9 cut(s) 665, 1666, 1973, 2431, 2800, 3494, 4187, 4267, 5338
AsuII TTCGAA 1 cut(s) 1392
AvaI CYCGRG 3 cut(s) 558, 1384, 2562
AvaII GGWCC 6 cut(s) 1169, 1186, 2225, 3223, 4014, 5213
AxyI CCTNAGG 1 cut(s) 909
BaeGI GKGCMC 1 cut(s) 5111
BaeI ACNNNNGTAYC 2 cut(s) 2925, 2958
BalI TGGCCA 3 cut(s) 750, 2082, 4246
BamHI GGATCC 2 cut(s) 2979, 4345
BanI GGYRCC 1 cut(s) 531
BanII GRGCYC 5 cut(s) 785, 1250, 1851, 1987, 2563
BauI CACGAG 3 cut(s) 1533, 1988, 3426
BbsI GAAGAC 5 cut(s) 227, 1692, 2502, 2640, 3491
Bbv12I GWGCWC 8 cut(s) 785, 1250, 2075, 2563, 2878, 3427, 4072, 5495
BbvCI CCTCAGC 2 cut(s) 3147, 4899
BceAI ACGGC 4 cut(s) 57, 882, 1608, 3588
BcgI CGANNNNNNTGC 2 cut(s) 1382, 1416
BciVI GTATCC 1 cut(s) 5583
BclI TGATCA 2 cut(s) 2179, 2476
BcnI CCSGG 3 cut(s) 1385, 1386, 3494
BcoDI GTCTC 9 cut(s) 57, 1426, 1494, 2114, 3570, 3720, 4022, 4499, 4596
BcuI ACTAGT 2 cut(s) 3344, 5387
BfmI CTRYAG 3 cut(s) 4536, 5217, 5586
BfrI CTTAAG 1 cut(s) 263
BfuAI ACCTGC 2 cut(s) 1807, 3752
BfuI GTATCC 1 cut(s) 5583
BglII AGATCT 1 cut(s) 1639
BlpI GCTNAGC 1 cut(s) 1656
BmcAI AGTACT 2 cut(s) 1276, 3694
Bme18I GGWCC 6 cut(s) 1169, 1186, 2225, 3223, 4014, 5213
BmeT110I CYCGRG 3 cut(s) 558, 1384, 2562
BmgT120I GGNCC 9 cut(s) 771, 1169, 1186, 2003, 2225, 2882, 3223, 4014, 5213
BmiI GGNNCC 8 cut(s) 533, 1188, 1833, 1984, 2981, 4347, 4831, 5044
BmrI ACTGGG 3 cut(s) 137, 1845, 4615
BmsI GCATC 6 cut(s) 95, 262, 2272, 5161, 5233, 5408
BmuI ACTGGG 3 cut(s) 137, 1845, 4615
BoxI GACNNNNGTC 1 cut(s) 2106
BpiI GAAGAC 5 cut(s) 227, 1692, 2502, 2640, 3491
BpmI CTGGAG 4 cut(s) 483, 2317, 2862, 4566
Bpu10I CCTNAGC 4 cut(s) 2229, 3067, 3147, 4899
Bpu1102I GCTNAGC 1 cut(s) 1656
Bpu14I TTCGAA 1 cut(s) 1392
BpuEI CTTGAG 4 cut(s) 789, 3390, 4044, 5458
BpuMI CCSGG 3 cut(s) 1385, 1386, 3494
Bsa29I ATCGAT 1 cut(s) 4984
BsaAI YACGTR 1 cut(s) 245
BsaBI GATNNNNATC 2 cut(s) 642, 2586
BsaHI GRCGYC 4 cut(s) 322, 844, 4585, 5070
BsaI GGTCTC 1 cut(s) 3720
BsaWI WCCGGW 4 cut(s) 415, 1474, 4016, 4717
BsaXI ACNNNNNCTCC 6 cut(s) 385, 415, 2062, 2092, 5257, 5287
Bse118I RCCGGY 1 cut(s) 1474
Bse1I ACTGG 6 cut(s) 143, 1438, 1851, 2981, 4382, 4610
Bse21I CCTNAGG 1 cut(s) 909
Bse3DI GCAATG 2 cut(s) 1650, 2691
Bse8I GATNNNNATC 2 cut(s) 642, 2586
BseAI TCCGGA 2 cut(s) 415, 4016
BseCI ATCGAT 1 cut(s) 4984
BseJI GATNNNNATC 2 cut(s) 642, 2586
BseMI GCAATG 2 cut(s) 1650, 2691
BseNI ACTGG 6 cut(s) 143, 1438, 1851, 2981, 4382, 4610
BseRI GAGGAG 5 cut(s) 897, 1065, 3135, 3393, 3494
BseSI GKGCMC 1 cut(s) 5111
BseYI CCCAGC 2 cut(s) 1281, 3893
BsgI GTGCAG 5 cut(s) 2049, 2649, 3114, 3543, 3678
Bsh1236I CGCG 6 cut(s) 153, 2427, 2459, 4000, 4642, 4960
BshNI GGYRCC 1 cut(s) 531
BshTI ACCGGT 1 cut(s) 1474
BshVI ATCGAT 1 cut(s) 4984
BsiHKAI GWGCWC 8 cut(s) 785, 1250, 2075, 2563, 2878, 3427, 4072, 5495
BsiHKCI CYCGRG 3 cut(s) 558, 1384, 2562
BsiSI CCGG 6 cut(s) 416, 1385, 1475, 3493, 4017, 4718
BslFI GGGAC 6 cut(s) 843, 1571, 3011, 3623, 5447, 5637
BsmAI GTCTC 9 cut(s) 57, 1426, 1494, 2114, 3570, 3720, 4022, 4499, 4596
BsmBI CGTCTC 2 cut(s) 57, 3570
BsmFI GGGAC 6 cut(s) 843, 1571, 3011, 3623, 5447, 5637
BsmI GAATGC 3 cut(s) 20, 4624, 5521
Bso31I GGTCTC 1 cut(s) 3720
BsoBI CYCGRG 3 cut(s) 558, 1384, 2562
Bsp119I TTCGAA 1 cut(s) 1392
Bsp13I TCCGGA 2 cut(s) 415, 4016
Bsp1407I TGTACA 1 cut(s) 4470
Bsp1720I GCTNAGC 1 cut(s) 1656
Bsp19I CCATGG 3 cut(s) 317, 1946, 5429
Bsp68I TCGCGA 2 cut(s) 2427, 2459
BspDI ATCGAT 1 cut(s) 4984
BspEI TCCGGA 2 cut(s) 415, 4016
BspFNI CGCG 6 cut(s) 153, 2427, 2459, 4000, 4642, 4960
BspLI GGNNCC 8 cut(s) 533, 1188, 1833, 1984, 2981, 4347, 4831, 5044
BspMAI CTGCAG 2 cut(s) 5221, 5590
BspMI ACCTGC 2 cut(s) 1807, 3752
BspQI GCTCTTC 5 cut(s) 773, 1752, 1929, 2166, 4272
BspT104I TTCGAA 1 cut(s) 1392
BspT107I GGYRCC 1 cut(s) 531
BspTI CTTAAG 1 cut(s) 263
BspTNI GGTCTC 1 cut(s) 3720
BsrBI CCGCTC 1 cut(s) 2668
BsrDI GCAATG 2 cut(s) 1650, 2691
BsrFI RCCGGY 1 cut(s) 1474
BsrGI TGTACA 1 cut(s) 4470
BsrI ACTGG 6 cut(s) 143, 1438, 1851, 2981, 4382, 4610
BssAI RCCGGY 1 cut(s) 1474
BssNI GRCGYC 4 cut(s) 322, 844, 4585, 5070
BssSI CACGAG 3 cut(s) 1533, 1988, 3426
BssT1I CCWWGG 7 cut(s) 317, 1413, 1946, 2619, 5098, 5168, 5429
Bst2BI CACGAG 3 cut(s) 1533, 1988, 3426
Bst4CI ACNGT 9 cut(s) 2364, 2707, 3188, 3564, 3650, 3736, 4048, 4748, 5007
BstACI GRCGYC 4 cut(s) 322, 844, 4585, 5070
BstAFI CTTAAG 1 cut(s) 263
BstAUI TGTACA 1 cut(s) 4470
BstBAI YACGTR 1 cut(s) 245
BstBI TTCGAA 1 cut(s) 1392
BstC8I GCNNGC 8 cut(s) 2425, 3133, 3966, 4682, 4815, 4927, 4931, 5610
BstDSI CCRYGG 6 cut(s) 317, 1946, 2077, 2464, 3604, 5429
BstENI CCTNNNNNAGG 1 cut(s) 3476
BstFNI CGCG 6 cut(s) 153, 2427, 2459, 4000, 4642, 4960
BstHHI GCGC 4 cut(s) 4000, 4644, 4960, 5128
BstMAI GTCTC 9 cut(s) 57, 1426, 1494, 2114, 3570, 3720, 4022, 4499, 4596
BstNSI RCATGY 3 cut(s) 12, 1400, 2952
BstPAI GACNNNNGTC 1 cut(s) 2106
BstSFI CTRYAG 3 cut(s) 4536, 5217, 5586
BstSLI GKGCMC 1 cut(s) 5111
BstSNI TACGTA 1 cut(s) 245
BstUI CGCG 6 cut(s) 153, 2427, 2459, 4000, 4642, 4960
BstV2I GAAGAC 5 cut(s) 227, 1692, 2502, 2640, 3491
BstX2I RGATCY 7 cut(s) 376, 643, 1639, 1714, 2979, 3445, 4345
BstXI CCANNNNNNTGG 1 cut(s) 1758
BstYI RGATCY 7 cut(s) 376, 643, 1639, 1714, 2979, 3445, 4345
Bsu15I ATCGAT 1 cut(s) 4984
Bsu36I CCTNAGG 1 cut(s) 909
BsuI GTATCC 1 cut(s) 5583
BsuTUI ATCGAT 1 cut(s) 4984
BtgI CCRYGG 6 cut(s) 317, 1946, 2077, 2464, 3604, 5429
BtsI GCAGTG 1 cut(s) 3555
BtuMI TCGCGA 2 cut(s) 2427, 2459
BveI ACCTGC 2 cut(s) 1807, 3752
Cac8I GCNNGC 8 cut(s) 2425, 3133, 3966, 4682, 4815, 4927, 4931, 5610
CaiI CAGNNNCTG 6 cut(s) 113, 2368, 2885, 4514, 4544, 5525
CfoI GCGC 4 cut(s) 4000, 4644, 4960, 5128
Cfr10I RCCGGY 1 cut(s) 1474
Cfr13I GGNCC 9 cut(s) 771, 1169, 1186, 2003, 2225, 2882, 3223, 4014, 5213
Cfr9I CCCGGG 1 cut(s) 1384
ClaI ATCGAT 1 cut(s) 4984
CseI GACGC 4 cut(s) 39, 311, 833, 5059
CsiI ACCWGGT 1 cut(s) 3166
CspAI ACCGGT 1 cut(s) 1474
CspCI CAANNNNNGTGG 4 cut(s) 651, 686, 2110, 2145
DraIII CACNNNGTG 1 cut(s) 1307
DrdI GACNNNNNNGTC 1 cut(s) 1193
DseDI GACNNNNNNGTC 1 cut(s) 1193
EaeI YGGCCR 3 cut(s) 748, 2080, 4244
Ecl136II GAGCTC 3 cut(s) 783, 1248, 2561
Eco105I TACGTA 1 cut(s) 245
Eco130I CCWWGG 7 cut(s) 317, 1413, 1946, 2619, 5098, 5168, 5429
Eco147I AGGCCT 1 cut(s) 33
Eco24I GRGCYC 5 cut(s) 785, 1250, 1851, 1987, 2563
Eco31I GGTCTC 1 cut(s) 3720
Eco32I GATATC 1 cut(s) 2455
Eco47I GGWCC 6 cut(s) 1169, 1186, 2225, 3223, 4014, 5213
Eco53kI GAGCTC 3 cut(s) 783, 1248, 2561
Eco57I CTGAAG 6 cut(s) 1143, 2496, 2670, 3648, 4325, 5483
Eco81I CCTNAGG 1 cut(s) 909
Eco88I CYCGRG 3 cut(s) 558, 1384, 2562
EcoICRI GAGCTC 3 cut(s) 783, 1248, 2561
EcoNI CCTNNNNNAGG 1 cut(s) 3476
EcoO109I RGGNCCY 3 cut(s) 771, 1186, 2882
EcoRV GATATC 1 cut(s) 2455
EcoT14I CCWWGG 7 cut(s) 317, 1413, 1946, 2619, 5098, 5168, 5429
EcoT38I GRGCYC 5 cut(s) 785, 1250, 1851, 1987, 2563
ErhI CCWWGG 7 cut(s) 317, 1413, 1946, 2619, 5098, 5168, 5429
Esp3I CGTCTC 2 cut(s) 57, 3570
FalI AAGNNNNNCTT 2 cut(s) 2584, 2616
FaqI GGGAC 6 cut(s) 843, 1571, 3011, 3623, 5447, 5637
FauI CCCGC 4 cut(s) 146, 3821, 4079, 5090
FauNDI CATATG 1 cut(s) 5147
FbaI TGATCA 2 cut(s) 2179, 2476
FblI GTMKAC 3 cut(s) 3172, 3769, 4535
FriOI GRGCYC 5 cut(s) 785, 1250, 1851, 1987, 2563
GlaI GCGC 4 cut(s) 3999, 4643, 4959, 5127
GsaI CCCAGC 2 cut(s) 1285, 3897
GsuI CTGGAG 4 cut(s) 483, 2317, 2862, 4566
HapII CCGG 6 cut(s) 416, 1385, 1475, 3493, 4017, 4718
HgaI GACGC 4 cut(s) 39, 311, 833, 5059
HhaI GCGC 4 cut(s) 4000, 4644, 4960, 5128
Hin1I GRCGYC 4 cut(s) 322, 844, 4585, 5070
Hin6I GCGC 4 cut(s) 3998, 4642, 4958, 5126
HinP1I GCGC 4 cut(s) 3998, 4642, 4958, 5126
HincII GTYRAC 4 cut(s) 1135, 1856, 5182, 5276
HindII GTYRAC 4 cut(s) 1135, 1856, 5182, 5276
HindIII AAGCTT 1 cut(s) 2133
HpaI GTTAAC 1 cut(s) 5182
HpaII CCGG 6 cut(s) 416, 1385, 1475, 3493, 4017, 4718
HphI GGTGA 9 cut(s) 665, 1666, 1973, 2431, 2800, 3494, 4187, 4267, 5338
Hpy99I CGWCG 1 cut(s) 1795
HpyCH4III ACNGT 9 cut(s) 2364, 2707, 3188, 3564, 3650, 3736, 4048, 4748, 5007
HpyCH4IV ACGT 9 cut(s) 244, 1937, 2412, 2754, 3175, 3628, 3742, 4585, 5140
HpySE526I ACGT 9 cut(s) 244, 1937, 2412, 2754, 3175, 3628, 3742, 4585, 5140
Hsp92I GRCGYC 4 cut(s) 322, 844, 4585, 5070
HspAI GCGC 4 cut(s) 3998, 4642, 4958, 5126
Kpn2I TCCGGA 2 cut(s) 415, 4016
Ksp22I TGATCA 2 cut(s) 2179, 2476
KspAI GTTAAC 1 cut(s) 5182
LguI GCTCTTC 5 cut(s) 773, 1752, 1929, 2166, 4272
LweI GCATC 6 cut(s) 95, 262, 2272, 5161, 5233, 5408
MabI ACCWGGT 1 cut(s) 3166
MaeII ACGT 9 cut(s) 244, 1937, 2412, 2754, 3175, 3628, 3742, 4585, 5140
MbiI CCGCTC 1 cut(s) 2668
MfeI CAATTG 4 cut(s) 200, 3060, 3524, 4282
MflI RGATCY 7 cut(s) 376, 643, 1639, 1714, 2979, 3445, 4345
MlsI TGGCCA 3 cut(s) 750, 2082, 4246
MluNI TGGCCA 3 cut(s) 750, 2082, 4246
MlyI GAGTC 9 cut(s) 636, 1589, 4024, 4051, 4541, 4766, 4962, 5241, 5619
Mox20I TGGCCA 3 cut(s) 750, 2082, 4246
MroI TCCGGA 2 cut(s) 415, 4016
MroXI GAANNNNTTC 5 cut(s) 209, 969, 1466, 4151, 4480
MscI TGGCCA 3 cut(s) 750, 2082, 4246
MslI CAYNNNNRTG 2 cut(s) 1917, 2613
Msp20I TGGCCA 3 cut(s) 750, 2082, 4246
MspA1I CMGCKG 3 cut(s) 3819, 4925, 5522
MspCI CTTAAG 1 cut(s) 263
MspI CCGG 6 cut(s) 416, 1385, 1475, 3493, 4017, 4718
MunI CAATTG 4 cut(s) 200, 3060, 3524, 4282
Mva1269I GAATGC 3 cut(s) 20, 4624, 5521
MvnI CGCG 6 cut(s) 153, 2427, 2459, 4000, 4642, 4960
NciI CCSGG 3 cut(s) 1385, 1386, 3494
NcoI CCATGG 3 cut(s) 317, 1946, 5429
NdeI CATATG 1 cut(s) 5147
NlaIV GGNNCC 8 cut(s) 533, 1188, 1833, 1984, 2981, 4347, 4831, 5044
NmuCI GTSAC 8 cut(s) 1961, 2437, 2788, 4042, 4100, 4586, 4880, 5057
NruI TCGCGA 2 cut(s) 2427, 2459
NspI RCATGY 3 cut(s) 12, 1400, 2952
NspV TTCGAA 1 cut(s) 1392
PaeR7I CTCGAG 1 cut(s) 2562
PasI CCCWGGG 1 cut(s) 15
PceI AGGCCT 1 cut(s) 33
PciI ACATGT 1 cut(s) 1396
PciSI GCTCTTC 5 cut(s) 773, 1752, 1929, 2166, 4272
PcsI WCGNNNNNNNCGW 1 cut(s) 47
PctI GAATGC 3 cut(s) 20, 4624, 5521
PdmI GAANNNNTTC 5 cut(s) 209, 969, 1466, 4151, 4480
PflMI CCANNNNNTGG 2 cut(s) 997, 5355
PinAI ACCGGT 1 cut(s) 1474
PleI GAGTC 9 cut(s) 635, 1589, 4024, 4051, 4540, 4765, 4962, 5240, 5619
PpsI GAGTC 9 cut(s) 635, 1589, 4024, 4051, 4540, 4765, 4962, 5240, 5619
Ppu21I YACGTR 1 cut(s) 245
PpuMI RGGWCCY 1 cut(s) 1186
PscI ACATGT 1 cut(s) 1396
PshAI GACNNNNGTC 1 cut(s) 2106
PshBI ATTAAT 2 cut(s) 1427, 3678
PsiI TTATAA 2 cut(s) 4572, 4908
Psp124BI GAGCTC 3 cut(s) 785, 1250, 2563
Psp1406I AACGTT 1 cut(s) 2412
Psp5II RGGWCCY 1 cut(s) 1186
PspFI CCCAGC 2 cut(s) 1281, 3893
PspN4I GGNNCC 8 cut(s) 533, 1188, 1833, 1984, 2981, 4347, 4831, 5044
PspPI GGNCC 9 cut(s) 771, 1169, 1186, 2003, 2225, 2882, 3223, 4014, 5213
PspPPI RGGWCCY 1 cut(s) 1186
PspXI VCTCGAGB 1 cut(s) 2562
PstI CTGCAG 2 cut(s) 5221, 5590
PstNI CAGNNNCTG 6 cut(s) 113, 2368, 2885, 4514, 4544, 5525
PsuI RGATCY 7 cut(s) 376, 643, 1639, 1714, 2979, 3445, 4345
PvuII CAGCTG 3 cut(s) 3819, 4925, 5522
RruI TCGCGA 2 cut(s) 2427, 2459
RseI CAYNNNNRTG 2 cut(s) 1917, 2613
SacI GAGCTC 3 cut(s) 785, 1250, 2563
SapI GCTCTTC 5 cut(s) 773, 1752, 1929, 2166, 4272
Sau96I GGNCC 9 cut(s) 771, 1169, 1186, 2003, 2225, 2882, 3223, 4014, 5213
ScaI AGTACT 2 cut(s) 1276, 3694
SchI GAGTC 9 cut(s) 636, 1589, 4024, 4051, 4541, 4766, 4962, 5241, 5619
SexAI ACCWGGT 1 cut(s) 3166
SfaNI GCATC 6 cut(s) 95, 262, 2272, 5161, 5233, 5408
SfcI CTRYAG 3 cut(s) 4536, 5217, 5586
Sfr274I CTCGAG 1 cut(s) 2562
SfuI TTCGAA 1 cut(s) 1392
SinI GGWCC 6 cut(s) 1169, 1186, 2225, 3223, 4014, 5213
SlaI CTCGAG 1 cut(s) 2562
SmaI CCCGGG 1 cut(s) 1386
SmiMI CAYNNNNRTG 2 cut(s) 1917, 2613
SmlI CTYRAG 6 cut(s) 263, 804, 2562, 3405, 4059, 5437
SmoI CTYRAG 6 cut(s) 263, 804, 2562, 3405, 4059, 5437
SnaBI TACGTA 1 cut(s) 245
SpeI ACTAGT 2 cut(s) 3344, 5387
SseBI AGGCCT 1 cut(s) 33
SspI AATATT 4 cut(s) 1201, 1345, 1618, 4024
SstI GAGCTC 3 cut(s) 785, 1250, 2563
StuI AGGCCT 1 cut(s) 33
StyI CCWWGG 7 cut(s) 317, 1413, 1946, 2619, 5098, 5168, 5429
TaaI ACNGT 9 cut(s) 2364, 2707, 3188, 3564, 3650, 3736, 4048, 4748, 5007
TaiI ACGT 9 cut(s) 247, 1940, 2415, 2757, 3178, 3631, 3745, 4588, 5143
TaqI TCGA 5 cut(s) 1392, 2563, 2590, 4374, 4984
TaqII GACCGA 2 cut(s) 1183, 3240
TatI WGTACW 7 cut(s) 407, 728, 808, 1274, 2594, 3692, 4470
TauI GCSGC 2 cut(s) 1745, 4127
TseFI GTSAC 8 cut(s) 1961, 2437, 2788, 4042, 4100, 4586, 4880, 5057
Tsp45I GTSAC 8 cut(s) 1961, 2437, 2788, 4042, 4100, 4586, 4880, 5057
TspGWI ACGGA 3 cut(s) 1464, 2645, 4244
TspMI CCCGGG 1 cut(s) 1384
Van91I CCANNNNNTGG 2 cut(s) 997, 5355
Vha464I CTTAAG 1 cut(s) 263
VpaK11BI GGWCC 6 cut(s) 1169, 1186, 2225, 3223, 4014, 5213
VspI ATTAAT 2 cut(s) 1427, 3678
XagI CCTNNNNNAGG 1 cut(s) 3476
XapI RAATTY 9 cut(s) 162, 423, 492, 956, 1708, 2269, 4359, 4479, 4947
XbaI TCTAGA 1 cut(s) 254
XceI RCATGY 3 cut(s) 12, 1400, 2952
XhoI CTCGAG 1 cut(s) 2562
XmaI CCCGGG 1 cut(s) 1384
XmiI GTMKAC 3 cut(s) 3172, 3769, 4535
XmnI GAANNNNTTC 5 cut(s) 209, 969, 1466, 4151, 4480
ZraI GACGTC 1 cut(s) 4586
ZrmI AGTACT 2 cut(s) 1276, 3694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.