FvH4_5g02200

DNA-binding protein ESCAROLA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0005616
Physical Location & Seq
Reverse (-)
1359221 .. 1363871
4651 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g02200.t1

Sequence Viewer

Length: 1068 bp
ATGGATGGGAGAGAAGCCATGGCCATCTCTGGTGCTCAAGCTTCCTACTACATCCATAGGGGAGGCCATGGTGGGTCTTCACCTGGATCACAGATTGCTGGGTTGCATGCACCACCTGGGTTCAGACCCAACAACAACGCTACCTTTCAGCAAGCTCAGTCTAATGTGAGAGCCACCTCTGTGGGATCAACATTCTCAGCAGAGCCTTCTAGACCCAATTATCCTCATGGCATTAGCATGAGTGGTTCTCCTGGAGCTCCTTCGAGTGAGCCTGTGAAGAAGAAACGAGGGAGGCCCCGGAAATATGGGCCTGGTCTTGGTTCCGGTCAGGGTCAGGGTCCTGATGCCCCGGTTTCGCTGGCATTGTCTCCATTGTCTTCAACTCCTAATCCTACTCCAGGATCAACCTCACCGACCCCGAAAAGAAACAGGGGAAGGCCACCTGGGACCGGCAGGAAACAACAGTTGGCTAATCTTGGGGAATGGATGAACAGTTCGGCTGGACAGGCTTTTGCACCCCATGTTATCACCATCGAAGCTGGAGAGGACATTGTAGCAAAATTGTTGTTATTTTCACAACAGAGGCCTAGGGCACTGTGTGTCTTGTCAGGAAATGGAACAGTATCTTCAGTAGCACTGCGTCAGCCTGCATCAACTGGTGTCAGTGTCACATATGAGGGTCGTTTCCAGATATTATGCTTGTCAGGTTCTTACTTGGTTGCGGAGGATGGTGGCCCGCGCGATCGAACAGGGGGCATAAGTGTTTCCCTTTCTAGTTCTGATGGTCATGTCATTGGTGGTGCTGTGGCTATGCTTATTGCAGCCTCTCCAGTGCAGGTTGTGCTTTGCAGTTTTGTATATGGTAGCTCCAACAAGATCAAGAGCAAGCCGCTGCCTGGCCCCAATAATGGGGAGAGTTCTGAGCCTCAGCACAGTGAGAAACTACCTTCACCGACAACAGCTCCATCTACTCAAAATTATGCTCCATCCACAGCAGGTATATGGCCTGGTGGGCGGCTAGTTGATGCAAGAAATGCTCACACTGGTATTGACTTGACTCGCGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0043621
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

356

Amino Acids

36.43

Weight (kDa)

9.96

Isoelectric Point (pI)

47.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 173 - 285 1.3e-22 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 826, 986
AccII CGCG 3 cut(s) 739, 741, 1062
AciI CCGC 5 cut(s) 722, 737, 890, 1015, 1062
AclWI GGATC 3 cut(s) 94, 193, 409
AcoI YGGCCR 1 cut(s) 21
AcuI CTGAAG 1 cut(s) 612
AdeI CACNNNGTG 1 cut(s) 599
AfiI CCNNNNNNNGG 6 cut(s) 317, 398, 449, 896, 908, 909
AgsI TTSAA 1 cut(s) 381
AjnI CCWGG 8 cut(s) 82, 115, 250, 310, 397, 442, 895, 1006
AjuI GAANNNNNNNTTGG 2 cut(s) 449, 481
AleI CACNNNNGTG 1 cut(s) 179
AloI GAACNNNNNNTCC 2 cut(s) 478, 510
AluBI AGCT 6 cut(s) 41, 155, 257, 539, 867, 962
AluI AGCT 6 cut(s) 41, 155, 257, 539, 867, 962
Alw21I GWGCWC 2 cut(s) 37, 259
Alw26I GTCTC 1 cut(s) 372
AlwI GGATC 3 cut(s) 94, 193, 409
AoxI GGCC 9 cut(s) 21, 64, 293, 308, 437, 584, 733, 898, 1004
ApeKI GCWGC 2 cut(s) 821, 892
AspA2I CCTAGG 1 cut(s) 587
AspLEI GCGC 1 cut(s) 741
AspS9I GGNCC 6 cut(s) 294, 308, 338, 447, 734, 899
AsuC2I CCSGG 2 cut(s) 298, 350
AsuHPI GGTGA 4 cut(s) 72, 402, 520, 942
AvaII GGWCC 2 cut(s) 338, 447
AvrII CCTAGG 1 cut(s) 587
BaeGI GKGCMC 1 cut(s) 595
BalI TGGCCA 1 cut(s) 23
BanII GRGCYC 1 cut(s) 259
BbsI GAAGAC 2 cut(s) 69, 369
Bbv12I GWGCWC 2 cut(s) 37, 259
BbvCI CCTCAGC 1 cut(s) 927
BbvI GCAGC 2 cut(s) 833, 879
BccI CCATC 6 cut(s) 32, 539, 722, 776, 973, 994
BciT130I CCWGG 8 cut(s) 84, 117, 252, 312, 399, 444, 897, 1008
BcnI CCSGG 2 cut(s) 298, 350
BcoDI GTCTC 1 cut(s) 372
BfaI CTAG 4 cut(s) 210, 588, 774, 1019
BfuAI ACCTGC 2 cut(s) 826, 986
BglI GCCNNNNNGGC 1 cut(s) 1012
BisI GCNGC 4 cut(s) 822, 890, 893, 1016
BlnI CCTAGG 1 cut(s) 587
BlsI GCNGC 4 cut(s) 823, 891, 894, 1017
Bme18I GGWCC 2 cut(s) 338, 447
BmgT120I GGNCC 6 cut(s) 294, 308, 338, 447, 734, 899
BmiI GGNNCC 5 cut(s) 296, 322, 339, 448, 901
BmsI GCATC 3 cut(s) 334, 659, 1015
BpiI GAAGAC 2 cut(s) 69, 369
BplI GAGNNNNNCTC 2 cut(s) 232, 264
BpmI CTGGAG 4 cut(s) 273, 381, 561, 813
Bpu10I CCTNAGC 1 cut(s) 927
BpuEI CTTGAG 1 cut(s) 21
BpuMI CCSGG 2 cut(s) 298, 350
BsaJI CCNNGG 7 cut(s) 18, 67, 116, 296, 348, 443, 587
BsaWI WCCGGW 1 cut(s) 323
BsaXI ACNNNNNCTCC 2 cut(s) 946, 976
Bsc4I CCNNNNNNNGG 6 cut(s) 317, 398, 449, 896, 908, 909
Bse118I RCCGGY 1 cut(s) 449
Bse1I ACTGG 3 cut(s) 661, 830, 1048
BseBI CCWGG 8 cut(s) 84, 117, 252, 312, 399, 444, 897, 1008
BseDI CCNNGG 7 cut(s) 18, 67, 116, 296, 348, 443, 587
BseGI GGATG 5 cut(s) 10, 51, 492, 733, 986
BseLI CCNNNNNNNGG 6 cut(s) 317, 398, 449, 896, 908, 909
BseMII CTCAG 4 cut(s) 170, 210, 912, 941
BseNI ACTGG 3 cut(s) 661, 830, 1048
BseSI GKGCMC 1 cut(s) 595
BseXI GCAGC 2 cut(s) 833, 879
BseYI CCCAGC 1 cut(s) 98
BsgI GTGCAG 1 cut(s) 854
Bsh1236I CGCG 3 cut(s) 739, 741, 1062
Bsh1285I CGRYCG 1 cut(s) 745
BshFI GGCC 9 cut(s) 23, 66, 295, 310, 439, 586, 735, 900, 1006
BsiEI CGRYCG 1 cut(s) 745
BsiHKAI GWGCWC 2 cut(s) 37, 259
BsiSI CCGG 4 cut(s) 298, 324, 350, 450
BslFI GGGAC 1 cut(s) 460
BslI CCNNNNNNNGG 6 cut(s) 317, 398, 449, 896, 908, 909
BsmAI GTCTC 1 cut(s) 372
BsmFI GGGAC 1 cut(s) 460
BsnI GGCC 9 cut(s) 23, 66, 295, 310, 439, 586, 735, 900, 1006
Bsp1286I GDGCHC 3 cut(s) 37, 259, 595
Bsp143I GATC 5 cut(s) 86, 185, 401, 742, 876
Bsp19I CCATGG 2 cut(s) 18, 67
BspACI CCGC 5 cut(s) 722, 737, 890, 1015, 1062
BspANI GGCC 9 cut(s) 23, 66, 295, 310, 439, 586, 735, 900, 1006
BspCNI CTCAG 4 cut(s) 169, 209, 913, 940
BspFNI CGCG 3 cut(s) 739, 741, 1062
BspLI GGNNCC 5 cut(s) 296, 322, 339, 448, 901
BspMI ACCTGC 2 cut(s) 826, 986
BspPI GGATC 3 cut(s) 94, 193, 409
BsrFI RCCGGY 1 cut(s) 449
BsrI ACTGG 3 cut(s) 661, 830, 1048
BssAI RCCGGY 1 cut(s) 449
BssECI CCNNGG 7 cut(s) 18, 67, 116, 296, 348, 443, 587
BssMI GATC 5 cut(s) 86, 185, 401, 742, 876
BssT1I CCWWGG 3 cut(s) 18, 67, 587
Bst2UI CCWGG 8 cut(s) 84, 117, 252, 312, 399, 444, 897, 1008
Bst4CI ACNGT 5 cut(s) 465, 494, 597, 622, 935
BstAPI GCANNNNNTGC 2 cut(s) 841, 1034
BstC8I GCNNGC 6 cut(s) 108, 153, 360, 648, 737, 887
BstDEI CTNAG 4 cut(s) 156, 196, 921, 927
BstDSI CCRYGG 2 cut(s) 18, 67
BstENI CCTNNNNNAGG 1 cut(s) 396
BstF5I GGATG 5 cut(s) 10, 51, 492, 733, 986
BstFNI CGCG 3 cut(s) 739, 741, 1062
BstHHI GCGC 1 cut(s) 741
BstKTI GATC 5 cut(s) 89, 188, 404, 745, 879
BstMAI GTCTC 1 cut(s) 372
BstMBI GATC 5 cut(s) 86, 185, 401, 742, 876
BstMCI CGRYCG 1 cut(s) 745
BstMWI GCNNNNNNNGC 4 cut(s) 506, 841, 1012, 1034
BstNI CCWGG 8 cut(s) 84, 117, 252, 312, 399, 444, 897, 1008
BstNSI RCATGY 1 cut(s) 110
BstSLI GKGCMC 1 cut(s) 595
BstUI CGCG 3 cut(s) 739, 741, 1062
BstV1I GCAGC 2 cut(s) 833, 879
BstV2I GAAGAC 2 cut(s) 69, 369
BstXI CCANNNNNNTGG 1 cut(s) 181
BsuRI GGCC 9 cut(s) 23, 66, 295, 310, 439, 586, 735, 900, 1006
BtgI CCRYGG 2 cut(s) 18, 67
BtsCI GGATG 5 cut(s) 10, 51, 492, 733, 986
BtsI GCAGTG 1 cut(s) 635
BtsIMutI CAGTG 6 cut(s) 593, 635, 670, 837, 940, 1041
BveI ACCTGC 2 cut(s) 826, 986
Cac8I GCNNGC 6 cut(s) 108, 153, 360, 648, 737, 887
CfoI GCGC 1 cut(s) 741
Cfr10I RCCGGY 1 cut(s) 449
Cfr13I GGNCC 6 cut(s) 294, 308, 338, 447, 734, 899
CseI GACGC 1 cut(s) 629
CviAII CATG 7 cut(s) 19, 68, 107, 227, 238, 521, 788
DdeI CTNAG 4 cut(s) 156, 196, 921, 927
DpnI GATC 5 cut(s) 88, 187, 403, 744, 878
DpnII GATC 5 cut(s) 86, 185, 401, 742, 876
DraIII CACNNNGTG 1 cut(s) 599
EaeI YGGCCR 1 cut(s) 21
Ecl136II GAGCTC 1 cut(s) 257
Eco130I CCWWGG 3 cut(s) 18, 67, 587
Eco147I AGGCCT 1 cut(s) 586
Eco24I GRGCYC 1 cut(s) 259
Eco47I GGWCC 2 cut(s) 338, 447
Eco53kI GAGCTC 1 cut(s) 257
Eco57I CTGAAG 1 cut(s) 612
EcoICRI GAGCTC 1 cut(s) 257
EcoNI CCTNNNNNAGG 1 cut(s) 396
EcoO109I RGGNCCY 2 cut(s) 294, 338
EcoRII CCWGG 8 cut(s) 82, 115, 250, 310, 397, 442, 895, 1006
EcoT14I CCWWGG 3 cut(s) 18, 67, 587
EcoT38I GRGCYC 1 cut(s) 259
ErhI CCWWGG 3 cut(s) 18, 67, 587
FaeI CATG 7 cut(s) 22, 71, 110, 230, 241, 524, 791
FaqI GGGAC 1 cut(s) 460
FatI CATG 7 cut(s) 18, 67, 106, 226, 237, 520, 787
FauI CCCGC 1 cut(s) 744
FauNDI CATATG 1 cut(s) 673
Fnu4HI GCNGC 4 cut(s) 822, 890, 893, 1016
FokI GGATG 5 cut(s) 17, 38, 499, 740, 973
FriOI GRGCYC 1 cut(s) 259
Fsp4HI GCNGC 4 cut(s) 822, 890, 893, 1016
FspBI CTAG 4 cut(s) 210, 588, 774, 1019
GlaI GCGC 1 cut(s) 740
GluI GCNGC 4 cut(s) 822, 890, 893, 1016
GsaI CCCAGC 1 cut(s) 102
GsuI CTGGAG 4 cut(s) 273, 381, 561, 813
HaeIII GGCC 9 cut(s) 23, 66, 295, 310, 439, 586, 735, 900, 1006
HapII CCGG 4 cut(s) 298, 324, 350, 450
HgaI GACGC 1 cut(s) 629
HhaI GCGC 1 cut(s) 741
Hin1II CATG 7 cut(s) 22, 71, 110, 230, 241, 524, 791
Hin6I GCGC 1 cut(s) 739
HinP1I GCGC 1 cut(s) 739
HindIII AAGCTT 1 cut(s) 39
HinfI GANTC 1 cut(s) 1057
HpaII CCGG 4 cut(s) 298, 324, 350, 450
HphI GGTGA 4 cut(s) 72, 402, 520, 942
Hpy188I TCNGA 3 cut(s) 125, 781, 922
Hpy188III TCNNGA 5 cut(s) 210, 341, 609, 688, 880
HpyAV CCTTC 4 cut(s) 216, 270, 429, 957
HpyCH4III ACNGT 5 cut(s) 465, 494, 597, 622, 935
HpyCH4V TGCA 8 cut(s) 106, 110, 515, 650, 821, 835, 849, 1028
HpyF10VI GCNNNNNNNGC 4 cut(s) 506, 841, 1012, 1034
HpyF3I CTNAG 4 cut(s) 156, 196, 921, 927
Hsp92II CATG 7 cut(s) 22, 71, 110, 230, 241, 524, 791
HspAI GCGC 1 cut(s) 739
Kzo9I GATC 5 cut(s) 86, 185, 401, 742, 876
LmnI GCTCC 5 cut(s) 254, 262, 872, 967, 988
Lsp1109I GCAGC 2 cut(s) 833, 879
LweI GCATC 3 cut(s) 334, 659, 1015
MaeI CTAG 4 cut(s) 210, 588, 774, 1019
MaeIII GTNAC 1 cut(s) 667
MalI GATC 5 cut(s) 88, 187, 403, 744, 878
MboI GATC 5 cut(s) 86, 185, 401, 742, 876
MboII GAAGA 5 cut(s) 69, 289, 292, 369, 618
MhlI GDGCHC 3 cut(s) 37, 259, 595
MlsI TGGCCA 1 cut(s) 23
MluCI AATT 3 cut(s) 217, 560, 976
MluNI TGGCCA 1 cut(s) 23
MlyI GAGTC 1 cut(s) 1051
MmeI TCCRAC 1 cut(s) 894
Mox20I TGGCCA 1 cut(s) 23
MscI TGGCCA 1 cut(s) 23
MslI CAYNNNNRTG 2 cut(s) 179, 236
Msp20I TGGCCA 1 cut(s) 23
MspA1I CMGCKG 1 cut(s) 892
MspI CCGG 4 cut(s) 298, 324, 350, 450
MvaI CCWGG 8 cut(s) 84, 117, 252, 312, 399, 444, 897, 1008
MvnI CGCG 3 cut(s) 739, 741, 1062
MwoI GCNNNNNNNGC 4 cut(s) 506, 841, 1012, 1034
NciI CCSGG 2 cut(s) 298, 350
NcoI CCATGG 2 cut(s) 18, 67
NdeI CATATG 1 cut(s) 673
NdeII GATC 5 cut(s) 86, 185, 401, 742, 876
NlaIII CATG 7 cut(s) 22, 71, 110, 230, 241, 524, 791
NlaIV GGNNCC 5 cut(s) 296, 322, 339, 448, 901
NmuCI GTSAC 1 cut(s) 667
NspI RCATGY 1 cut(s) 110
OliI CACNNNNGTG 1 cut(s) 179
PaeI GCATGC 1 cut(s) 110
PceI AGGCCT 1 cut(s) 586
PfoI TCCNGGA 2 cut(s) 250, 397
PkrI GCNGC 4 cut(s) 823, 891, 894, 1017
Ple19I CGATCG 1 cut(s) 745
PleI GAGTC 1 cut(s) 1051
PpsI GAGTC 1 cut(s) 1051
PpuMI RGGWCCY 1 cut(s) 338
Psp124BI GAGCTC 1 cut(s) 259
Psp5II RGGWCCY 1 cut(s) 338
Psp6I CCWGG 8 cut(s) 82, 115, 250, 310, 397, 442, 895, 1006
PspFI CCCAGC 1 cut(s) 98
PspGI CCWGG 8 cut(s) 82, 115, 250, 310, 397, 442, 895, 1006
PspN4I GGNNCC 5 cut(s) 296, 322, 339, 448, 901
PspPI GGNCC 6 cut(s) 294, 308, 338, 447, 734, 899
PspPPI RGGWCCY 1 cut(s) 338
PvuI CGATCG 1 cut(s) 745
RseI CAYNNNNRTG 2 cut(s) 179, 236
SacI GAGCTC 1 cut(s) 259
SatI GCNGC 4 cut(s) 822, 890, 893, 1016
Sau3AI GATC 5 cut(s) 86, 185, 401, 742, 876
Sau96I GGNCC 6 cut(s) 294, 308, 338, 447, 734, 899
SchI GAGTC 1 cut(s) 1051
SduI GDGCHC 3 cut(s) 37, 259, 595
SfaNI GCATC 3 cut(s) 334, 659, 1015
SinI GGWCC 2 cut(s) 338, 447
SmiMI CAYNNNNRTG 2 cut(s) 179, 236
SmlI CTYRAG 1 cut(s) 36
SmoI CTYRAG 1 cut(s) 36
SphI GCATGC 1 cut(s) 110
Sse9I AATT 3 cut(s) 217, 560, 976
SseBI AGGCCT 1 cut(s) 586
SsiI CCGC 5 cut(s) 722, 737, 890, 1015, 1062
SspMI CTAG 4 cut(s) 210, 588, 774, 1019
SstI GAGCTC 1 cut(s) 259
StuI AGGCCT 1 cut(s) 586
StyI CCWWGG 3 cut(s) 18, 67, 587
TaaI ACNGT 5 cut(s) 465, 494, 597, 622, 935
TaqI TCGA 3 cut(s) 263, 534, 745
TasI AATT 3 cut(s) 217, 560, 976
TauI GCSGC 2 cut(s) 892, 1018
TscAI CASTG 6 cut(s) 600, 642, 670, 837, 940, 1048
TseFI GTSAC 1 cut(s) 667
TseI GCWGC 2 cut(s) 821, 892
Tsp45I GTSAC 1 cut(s) 667
TspDTI ATGAA 1 cut(s) 503
TspRI CASTG 6 cut(s) 600, 642, 670, 837, 940, 1048
VpaK11BI GGWCC 2 cut(s) 338, 447
XagI CCTNNNNNAGG 1 cut(s) 396
XbaI TCTAGA 1 cut(s) 209
XceI RCATGY 1 cut(s) 110
XmaJI CCTAGG 1 cut(s) 587
XspI CTAG 4 cut(s) 210, 588, 774, 1019
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.