Rroxscaffold_3G00258100

DNA-binding protein ESCAROLA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0005616
Physical Location & Seq
Reverse (-)
52433454 .. 52438252
4799 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00258100.1

Sequence Viewer

Length: 1065 bp
ATGGATGGGAGAGAAGCCATGGCCATCTCTGGTGCTCAAGCTTCATACTACATCCATAGGGGAGGCCATGGTGGGTCTTCACCTGGATCACAGATTGCTGGGCTGCATGCACCACCTGGGTTCAGGCCCAACAACACTGCCTTTCAACAAGCTCAGACTAATGTGAGGGCCACCTCAGCATTCTCAGTAGAGCCTTCTTCAAGACCCAATTACCCTCATGGCATTAGCATCAATGTTTCTCCCGGAGGAGCTCCTTCTTCCGGTGAGCCTGTAAAGAAGAAACGAGGGAGGCCCCGGAAGTATGGGCCTGGTCCTGCTCCTGCTCCTGGTTCGGCTCCTGATGCCCCGGTTTCTCTGGCATTGTCTCCAATGTCTTCAACTCCTAATCCTGCTCCAGGATCAACCTCACCGACTCCGAAAAGAAACAGAGGAAGGCCACCTGGGACTGGCAGGAAACAACAATTGGCTAATCTTGGGGAATGGATGAACAGTTCGGCTGGACAGGCTTTTGCACCTCATGTTATCACCATCGAAGCTGGAGAGGACATTGCAGCAAAATTATTGTTATTTTCACAACAGAGGCCAAGGGCACTATGCATCTTATCAGGAAATGGAACAGTATCTTCAGTAGCACTGCGTCAGCCTGCATCAACTGGTGTCAGTGTCACATATGAGGGTCGTTTCCAGATATTATGCTTGTCAGGTTCTTACCTGGTTGCTGAGGATGGTGGTCCCCGCGATCGAACAGGGGGGATAAGCGTTTCCCTTTCTAGTTCTGATGGTCATGTCATTGGTGGTGCTGTGGCTATGCTTATTGCAGCAACTCCAGTGCAGATTGTGCTTTGCAGTTTTGTATATGGTAGCTCCAACAAGATCAAGAGCAAGCCAGTGGCTGACCCCAATAATGGGCAGAGTTCTGAGGCCCAGCACAGTGAGAAATTACCTTCACCGGCTACAGCTCCATCTACTCAAAATTATGCTCCATCCACAGCAGGCATTTGGCCTGGGGGGCGGCTAGTTGATGCGAGAAATGCTCACACCGGTATTGACTTGACTCGCGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0043621
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

354

Amino Acids

36.27

Weight (kDa)

9.96

Isoelectric Point (pI)

48.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 172 - 284 2.3e-22 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 738, 1059
AciI CCGC 3 cut(s) 736, 1012, 1059
AclWI GGATC 2 cut(s) 94, 406
AcoI YGGCCR 1 cut(s) 21
AcuI CTGAAG 1 cut(s) 609
AfiI CCNNNNNNNGG 6 cut(s) 260, 326, 395, 446, 905, 906
AgeI ACCGGT 1 cut(s) 1040
AgsI TTSAA 3 cut(s) 146, 201, 378
AjnI CCWGG 8 cut(s) 82, 115, 307, 325, 394, 439, 711, 1003
AjuI GAANNNNNNNTTGG 2 cut(s) 446, 478
AloI GAACNNNNNNTCC 2 cut(s) 475, 507
AluBI AGCT 6 cut(s) 41, 152, 251, 536, 864, 959
AluI AGCT 6 cut(s) 41, 152, 251, 536, 864, 959
Alw21I GWGCWC 2 cut(s) 37, 253
Alw26I GTCTC 1 cut(s) 369
AlwI GGATC 2 cut(s) 94, 406
AlwNI CAGNNNCTG 1 cut(s) 893
ApeKI GCWGC 3 cut(s) 103, 551, 818
AsiGI ACCGGT 1 cut(s) 1040
AspS9I GGNCC 7 cut(s) 126, 168, 291, 305, 311, 731, 922
AsuC2I CCSGG 3 cut(s) 243, 295, 347
AsuHPI GGTGA 5 cut(s) 72, 275, 399, 517, 939
AvaII GGWCC 2 cut(s) 311, 731
BaeGI GKGCMC 1 cut(s) 592
BalI TGGCCA 1 cut(s) 23
BanII GRGCYC 1 cut(s) 253
BbsI GAAGAC 2 cut(s) 69, 366
Bbv12I GWGCWC 2 cut(s) 37, 253
BbvCI CCTCAGC 2 cut(s) 175, 720
BbvI GCAGC 3 cut(s) 90, 563, 830
BccI CCATC 6 cut(s) 32, 536, 719, 773, 970, 991
BciT130I CCWGG 8 cut(s) 84, 117, 309, 327, 396, 441, 713, 1005
BcnI CCSGG 3 cut(s) 243, 295, 347
BcoDI GTCTC 1 cut(s) 369
BfaI CTAG 2 cut(s) 771, 1016
BfmI CTRYAG 1 cut(s) 954
BglI GCCNNNNNGGC 1 cut(s) 1009
BisI GCNGC 4 cut(s) 104, 552, 819, 1013
BlsI GCNGC 4 cut(s) 105, 553, 820, 1014
Bme18I GGWCC 2 cut(s) 311, 731
BmgT120I GGNCC 7 cut(s) 126, 168, 291, 305, 311, 731, 922
BmiI GGNNCC 3 cut(s) 293, 336, 733
BmsI GCATC 5 cut(s) 237, 331, 606, 656, 1012
BpiI GAAGAC 2 cut(s) 69, 366
BplI GAGNNNNNCTC 2 cut(s) 1018, 1050
BpmI CTGGAG 3 cut(s) 378, 558, 810
Bpu10I CCTNAGC 2 cut(s) 175, 720
BpuEI CTTGAG 1 cut(s) 21
BpuMI CCSGG 3 cut(s) 243, 295, 347
BsaJI CCNNGG 8 cut(s) 18, 67, 116, 293, 345, 440, 584, 1004
BsaWI WCCGGW 2 cut(s) 260, 1040
Bsc4I CCNNNNNNNGG 6 cut(s) 260, 326, 395, 446, 905, 906
Bse118I RCCGGY 2 cut(s) 949, 1040
Bse1I ACTGG 4 cut(s) 451, 658, 827, 887
Bse3DI GCAATG 1 cut(s) 546
BseBI CCWGG 8 cut(s) 84, 117, 309, 327, 396, 441, 713, 1005
BseDI CCNNGG 8 cut(s) 18, 67, 116, 293, 345, 440, 584, 1004
BseGI GGATG 5 cut(s) 10, 51, 489, 730, 983
BseLI CCNNNNNNNGG 6 cut(s) 260, 326, 395, 446, 905, 906
BseMI GCAATG 1 cut(s) 546
BseMII CTCAG 5 cut(s) 167, 189, 198, 711, 909
BseNI ACTGG 4 cut(s) 451, 658, 827, 887
BseRI GAGGAG 1 cut(s) 261
BseSI GKGCMC 1 cut(s) 592
BseXI GCAGC 3 cut(s) 90, 563, 830
BseYI CCCAGC 2 cut(s) 98, 924
BsgI GTGCAG 1 cut(s) 851
Bsh1236I CGCG 2 cut(s) 738, 1059
Bsh1285I CGRYCG 1 cut(s) 742
BshTI ACCGGT 1 cut(s) 1040
BsiEI CGRYCG 1 cut(s) 742
BsiHKAI GWGCWC 2 cut(s) 37, 253
BsiSI CCGG 6 cut(s) 243, 261, 295, 347, 950, 1041
BslFI GGGAC 2 cut(s) 457, 717
BslI CCNNNNNNNGG 6 cut(s) 260, 326, 395, 446, 905, 906
BsmAI GTCTC 1 cut(s) 369
BsmFI GGGAC 2 cut(s) 457, 717
BsmI GAATGC 1 cut(s) 179
Bsp1286I GDGCHC 3 cut(s) 37, 253, 592
Bsp143I GATC 4 cut(s) 86, 398, 739, 873
Bsp19I CCATGG 2 cut(s) 18, 67
BspACI CCGC 3 cut(s) 736, 1012, 1059
BspCNI CTCAG 5 cut(s) 166, 188, 197, 712, 910
BspFNI CGCG 2 cut(s) 738, 1059
BspLI GGNNCC 3 cut(s) 293, 336, 733
BspPI GGATC 2 cut(s) 94, 406
BsrDI GCAATG 1 cut(s) 546
BsrFI RCCGGY 2 cut(s) 949, 1040
BsrI ACTGG 4 cut(s) 451, 658, 827, 887
BssAI RCCGGY 2 cut(s) 949, 1040
BssECI CCNNGG 8 cut(s) 18, 67, 116, 293, 345, 440, 584, 1004
BssMI GATC 4 cut(s) 86, 398, 739, 873
BssT1I CCWWGG 3 cut(s) 18, 67, 584
Bst2UI CCWGG 8 cut(s) 84, 117, 309, 327, 396, 441, 713, 1005
Bst4CI ACNGT 3 cut(s) 491, 619, 932
BstAPI GCANNNNNTGC 1 cut(s) 838
BstC8I GCNNGC 4 cut(s) 108, 645, 884, 994
BstDEI CTNAG 5 cut(s) 153, 175, 184, 720, 918
BstDSI CCRYGG 2 cut(s) 18, 67
BstENI CCTNNNNNAGG 1 cut(s) 393
BstF5I GGATG 5 cut(s) 10, 51, 489, 730, 983
BstFNI CGCG 2 cut(s) 738, 1059
BstKTI GATC 4 cut(s) 89, 401, 742, 876
BstMAI GTCTC 1 cut(s) 369
BstMBI GATC 4 cut(s) 86, 398, 739, 873
BstMCI CGRYCG 1 cut(s) 742
BstMWI GCNNNNNNNGC 6 cut(s) 176, 341, 503, 838, 1009, 1031
BstNI CCWGG 8 cut(s) 84, 117, 309, 327, 396, 441, 713, 1005
BstNSI RCATGY 1 cut(s) 110
BstSFI CTRYAG 1 cut(s) 954
BstSLI GKGCMC 1 cut(s) 592
BstUI CGCG 2 cut(s) 738, 1059
BstV1I GCAGC 3 cut(s) 90, 563, 830
BstV2I GAAGAC 2 cut(s) 69, 366
BtgI CCRYGG 2 cut(s) 18, 67
BtsCI GGATG 5 cut(s) 10, 51, 489, 730, 983
BtsI GCAGTG 2 cut(s) 135, 632
BtsIMutI CAGTG 6 cut(s) 135, 632, 667, 834, 894, 937
Cac8I GCNNGC 4 cut(s) 108, 645, 884, 994
CaiI CAGNNNCTG 1 cut(s) 893
Cfr10I RCCGGY 2 cut(s) 949, 1040
Cfr13I GGNCC 7 cut(s) 126, 168, 291, 305, 311, 731, 922
CseI GACGC 1 cut(s) 626
CsiI ACCWGGT 1 cut(s) 711
CspAI ACCGGT 1 cut(s) 1040
CviAII CATG 6 cut(s) 19, 68, 107, 218, 518, 785
DdeI CTNAG 5 cut(s) 153, 175, 184, 720, 918
DpnI GATC 4 cut(s) 88, 400, 741, 875
DpnII GATC 4 cut(s) 86, 398, 739, 873
EaeI YGGCCR 1 cut(s) 21
Ecl136II GAGCTC 1 cut(s) 251
Eco130I CCWWGG 3 cut(s) 18, 67, 584
Eco24I GRGCYC 1 cut(s) 253
Eco47I GGWCC 2 cut(s) 311, 731
Eco53kI GAGCTC 1 cut(s) 251
Eco57I CTGAAG 1 cut(s) 609
EcoICRI GAGCTC 1 cut(s) 251
EcoNI CCTNNNNNAGG 1 cut(s) 393
EcoO109I RGGNCCY 1 cut(s) 291
EcoRII CCWGG 8 cut(s) 82, 115, 307, 325, 394, 439, 711, 1003
EcoT14I CCWWGG 3 cut(s) 18, 67, 584
EcoT22I ATGCAT 1 cut(s) 599
EcoT38I GRGCYC 1 cut(s) 253
ErhI CCWWGG 3 cut(s) 18, 67, 584
FaeI CATG 6 cut(s) 22, 71, 110, 221, 521, 788
FaqI GGGAC 2 cut(s) 457, 717
FatI CATG 6 cut(s) 18, 67, 106, 217, 517, 784
FauI CCCGC 1 cut(s) 743
FauNDI CATATG 1 cut(s) 670
Fnu4HI GCNGC 4 cut(s) 104, 552, 819, 1013
FokI GGATG 5 cut(s) 17, 38, 496, 737, 970
FriOI GRGCYC 1 cut(s) 253
Fsp4HI GCNGC 4 cut(s) 104, 552, 819, 1013
FspBI CTAG 2 cut(s) 771, 1016
GluI GCNGC 4 cut(s) 104, 552, 819, 1013
GsaI CCCAGC 2 cut(s) 102, 928
GsuI CTGGAG 3 cut(s) 378, 558, 810
HapII CCGG 6 cut(s) 243, 261, 295, 347, 950, 1041
HgaI GACGC 1 cut(s) 626
Hin1II CATG 6 cut(s) 22, 71, 110, 221, 521, 788
HindIII AAGCTT 1 cut(s) 39
HinfI GANTC 2 cut(s) 412, 1054
HpaII CCGG 6 cut(s) 243, 261, 295, 347, 950, 1041
HphI GGTGA 5 cut(s) 72, 275, 399, 517, 939
Hpy188I TCNGA 4 cut(s) 156, 417, 778, 919
Hpy188III TCNNGA 5 cut(s) 201, 338, 606, 685, 877
HpyAV CCTTC 4 cut(s) 204, 264, 426, 954
HpyCH4III ACNGT 3 cut(s) 491, 619, 932
HpyCH4V TGCA 9 cut(s) 106, 110, 512, 551, 597, 647, 818, 832, 846
HpyF10VI GCNNNNNNNGC 6 cut(s) 176, 341, 503, 838, 1009, 1031
HpyF3I CTNAG 5 cut(s) 153, 175, 184, 720, 918
Hsp92II CATG 6 cut(s) 22, 71, 110, 221, 521, 788
Kzo9I GATC 4 cut(s) 86, 398, 739, 873
LmnI GCTCC 9 cut(s) 248, 256, 322, 328, 340, 397, 869, 964, 985
Lsp1109I GCAGC 3 cut(s) 90, 563, 830
LweI GCATC 5 cut(s) 237, 331, 606, 656, 1012
MabI ACCWGGT 1 cut(s) 711
MaeI CTAG 2 cut(s) 771, 1016
MaeIII GTNAC 1 cut(s) 664
MalI GATC 4 cut(s) 88, 400, 741, 875
MboI GATC 4 cut(s) 86, 398, 739, 873
MboII GAAGA 6 cut(s) 69, 189, 249, 289, 366, 615
MfeI CAATTG 1 cut(s) 461
MhlI GDGCHC 3 cut(s) 37, 253, 592
MlsI TGGCCA 1 cut(s) 23
MluCI AATT 5 cut(s) 208, 461, 557, 938, 973
MluNI TGGCCA 1 cut(s) 23
MlyI GAGTC 2 cut(s) 406, 1048
MmeI TCCRAC 1 cut(s) 891
Mox20I TGGCCA 1 cut(s) 23
Mph1103I ATGCAT 1 cut(s) 599
MscI TGGCCA 1 cut(s) 23
Msp20I TGGCCA 1 cut(s) 23
MspI CCGG 6 cut(s) 243, 261, 295, 347, 950, 1041
MunI CAATTG 1 cut(s) 461
Mva1269I GAATGC 1 cut(s) 179
MvaI CCWGG 8 cut(s) 84, 117, 309, 327, 396, 441, 713, 1005
MvnI CGCG 2 cut(s) 738, 1059
MwoI GCNNNNNNNGC 6 cut(s) 176, 341, 503, 838, 1009, 1031
NciI CCSGG 3 cut(s) 243, 295, 347
NcoI CCATGG 2 cut(s) 18, 67
NdeI CATATG 1 cut(s) 670
NdeII GATC 4 cut(s) 86, 398, 739, 873
NlaIII CATG 6 cut(s) 22, 71, 110, 221, 521, 788
NlaIV GGNNCC 3 cut(s) 293, 336, 733
NmuCI GTSAC 1 cut(s) 664
NsiI ATGCAT 1 cut(s) 599
NspI RCATGY 1 cut(s) 110
PaeI GCATGC 1 cut(s) 110
PctI GAATGC 1 cut(s) 179
PfoI TCCNGGA 2 cut(s) 241, 394
PinAI ACCGGT 1 cut(s) 1040
PkrI GCNGC 4 cut(s) 105, 553, 820, 1014
Ple19I CGATCG 1 cut(s) 742
PleI GAGTC 2 cut(s) 406, 1048
PpsI GAGTC 2 cut(s) 406, 1048
Psp124BI GAGCTC 1 cut(s) 253
Psp6I CCWGG 8 cut(s) 82, 115, 307, 325, 394, 439, 711, 1003
PspFI CCCAGC 2 cut(s) 98, 924
PspGI CCWGG 8 cut(s) 82, 115, 307, 325, 394, 439, 711, 1003
PspN4I GGNNCC 3 cut(s) 293, 336, 733
PspPI GGNCC 7 cut(s) 126, 168, 291, 305, 311, 731, 922
PstNI CAGNNNCTG 1 cut(s) 893
PvuI CGATCG 1 cut(s) 742
SacI GAGCTC 1 cut(s) 253
SatI GCNGC 4 cut(s) 104, 552, 819, 1013
Sau3AI GATC 4 cut(s) 86, 398, 739, 873
Sau96I GGNCC 7 cut(s) 126, 168, 291, 305, 311, 731, 922
SchI GAGTC 2 cut(s) 406, 1048
SduI GDGCHC 3 cut(s) 37, 253, 592
SexAI ACCWGGT 1 cut(s) 711
SfaNI GCATC 5 cut(s) 237, 331, 606, 656, 1012
SfcI CTRYAG 1 cut(s) 954
SinI GGWCC 2 cut(s) 311, 731
SmlI CTYRAG 1 cut(s) 36
SmoI CTYRAG 1 cut(s) 36
SphI GCATGC 1 cut(s) 110
Sse9I AATT 5 cut(s) 208, 461, 557, 938, 973
SsiI CCGC 3 cut(s) 736, 1012, 1059
SspMI CTAG 2 cut(s) 771, 1016
SstI GAGCTC 1 cut(s) 253
StyI CCWWGG 3 cut(s) 18, 67, 584
TaaI ACNGT 3 cut(s) 491, 619, 932
TaqI TCGA 2 cut(s) 531, 742
TasI AATT 5 cut(s) 208, 461, 557, 938, 973
TauI GCSGC 1 cut(s) 1015
TscAI CASTG 6 cut(s) 142, 639, 667, 834, 894, 937
TseFI GTSAC 1 cut(s) 664
TseI GCWGC 3 cut(s) 103, 551, 818
Tsp45I GTSAC 1 cut(s) 664
TspDTI ATGAA 2 cut(s) 33, 500
TspRI CASTG 6 cut(s) 142, 639, 667, 834, 894, 937
VpaK11BI GGWCC 2 cut(s) 311, 731
XagI CCTNNNNNAGG 1 cut(s) 393
XceI RCATGY 1 cut(s) 110
XspI CTAG 2 cut(s) 771, 1016
Zsp2I ATGCAT 1 cut(s) 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.