Rw7G014670

DNA-binding protein ESCAROLA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0005616
Physical Location & Seq
Forward (+)
14389295 .. 14392888
3594 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G014670.1

Sequence Viewer

Length: 825 bp
ATGGATGGGAGAGAAGCCATGGCCATCTCTGGTGCTCAAGCTTCATACTACATCCATAGGGGAGGCCATGGTGGGTCTTCTCCTGGATCACAGATTGCTGGGCTGCATGCACCCCCTGGGTTCAGGCCCAACAACACTGCCTTTCAACAAGCTCAGTCTAATGTGAGGGCCTCCTCAGCATTCTCAGTAGAGCCTTCTTCGAGACCCAATTACCCTCATGGCATTAGCATCAATGTTTCTCCCGGAGGAGCTCCTTCTTCCGGTGAGCCTGTCAAGAAGAAACGAGGGAGGCCCCGGAAGTATGGCCCTGGTTCTGCTCCTGGCCCTGCCTCGGCTCCTGATGCCCCGGTTTCTCTGGCATTGTCTCCAATGTCTTCAACTCCTAATCCTACTCCAGGATCAACCTCACCGACTCCGAAACGAAACAGAGGAAGGCCACCTGGGACTGGCAGGAAACAACAATTGGCTAATCTTGGGGAATGGATGAACAGTTCGGCTGGACAGGCTTTTGCACCCCATGTTATCACCATTGAAGCTGGAGAGGACATTGCAGCAAAATTATTGTTATTTTCACAACAGAGGCCAAGGGCACTATGCATCTTGTCAGGAAATGGAACAGTATCTTCAGTAGCACTGCGTCAGCCTGCATCAACTGGTGTCAGTGTCACATATGAGGTTGAAGCACTCAACTCTGTCTTAATTTCATTTTACATGCCTTTCCTTCTGTGTTGGACATTTAAGATTTTGAGAATTTCAAAATTTGCATATAAAGCTGTATATTTTCTTCCTCTTTGCAAAGCATTTGTTGCAAATAGCAGAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0043621
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

274

Amino Acids

28.79

Weight (kDa)

10.31

Isoelectric Point (pI)

54.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 172 - 213 3e-08 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 94, 406
AcoI YGGCCR 1 cut(s) 21
AcsI RAATTY 2 cut(s) 750, 758
AcuI CTGAAG 1 cut(s) 609
AfiI CCNNNNNNNGG 4 cut(s) 260, 331, 395, 446
AgsI TTSAA 5 cut(s) 146, 378, 533, 680, 756
AjnI CCWGG 6 cut(s) 82, 115, 307, 319, 394, 439
AjuI GAANNNNNNNTTGG 2 cut(s) 446, 478
AloI GAACNNNNNNTCC 2 cut(s) 475, 507
AluBI AGCT 5 cut(s) 41, 152, 251, 536, 773
AluI AGCT 5 cut(s) 41, 152, 251, 536, 773
Alw21I GWGCWC 2 cut(s) 37, 253
Alw26I GTCTC 2 cut(s) 196, 369
AlwI GGATC 2 cut(s) 94, 406
AoxI GGCC 9 cut(s) 21, 64, 125, 168, 290, 304, 322, 434, 581
ApeKI GCWGC 2 cut(s) 103, 551
ApoI RAATTY 2 cut(s) 750, 758
AspS9I GGNCC 5 cut(s) 126, 168, 291, 305, 323
AsuC2I CCSGG 3 cut(s) 243, 295, 347
AsuHPI GGTGA 3 cut(s) 275, 399, 517
BaeGI GKGCMC 1 cut(s) 592
BalI TGGCCA 1 cut(s) 23
BanII GRGCYC 1 cut(s) 253
BarI GAAGNNNNNNTAC 2 cut(s) 768, 800
BbsI GAAGAC 2 cut(s) 69, 366
Bbv12I GWGCWC 2 cut(s) 37, 253
BbvCI CCTCAGC 1 cut(s) 175
BbvI GCAGC 2 cut(s) 90, 563
BccI CCATC 1 cut(s) 32
BciT130I CCWGG 6 cut(s) 84, 117, 309, 321, 396, 441
BcnI CCSGG 3 cut(s) 243, 295, 347
BcoDI GTCTC 2 cut(s) 196, 369
BisI GCNGC 2 cut(s) 104, 552
BlsI GCNGC 2 cut(s) 105, 553
Bme1390I CCNGG 9 cut(s) 84, 117, 243, 295, 309, 321, 347, 396, 441
BmgT120I GGNCC 5 cut(s) 126, 168, 291, 305, 323
BmiI GGNNCC 2 cut(s) 293, 336
BmrFI CCNGG 9 cut(s) 84, 117, 243, 295, 309, 321, 347, 396, 441
BmsI GCATC 4 cut(s) 237, 331, 606, 656
BpiI GAAGAC 2 cut(s) 69, 366
BpmI CTGGAG 2 cut(s) 378, 558
Bpu10I CCTNAGC 1 cut(s) 175
BpuEI CTTGAG 1 cut(s) 21
BpuMI CCSGG 3 cut(s) 243, 295, 347
BsaI GGTCTC 1 cut(s) 196
BsaWI WCCGGW 1 cut(s) 260
Bsc4I CCNNNNNNNGG 4 cut(s) 260, 331, 395, 446
Bse1I ACTGG 2 cut(s) 451, 658
Bse3DI GCAATG 1 cut(s) 546
BseBI CCWGG 6 cut(s) 84, 117, 309, 321, 396, 441
BseGI GGATG 3 cut(s) 10, 51, 489
BseLI CCNNNNNNNGG 4 cut(s) 260, 331, 395, 446
BseMI GCAATG 1 cut(s) 546
BseMII CTCAG 3 cut(s) 167, 189, 198
BseNI ACTGG 2 cut(s) 451, 658
BseRI GAGGAG 2 cut(s) 163, 261
BseSI GKGCMC 1 cut(s) 592
BseXI GCAGC 2 cut(s) 90, 563
BseYI CCCAGC 1 cut(s) 98
BshFI GGCC 9 cut(s) 23, 66, 127, 170, 292, 306, 324, 436, 583
BsiHKAI GWGCWC 2 cut(s) 37, 253
BsiSI CCGG 4 cut(s) 243, 261, 295, 347
BslFI GGGAC 1 cut(s) 457
BslI CCNNNNNNNGG 4 cut(s) 260, 331, 395, 446
BsmAI GTCTC 2 cut(s) 196, 369
BsmFI GGGAC 1 cut(s) 457
BsmI GAATGC 1 cut(s) 179
BsnI GGCC 9 cut(s) 23, 66, 127, 170, 292, 306, 324, 436, 583
Bso31I GGTCTC 1 cut(s) 196
Bsp1286I GDGCHC 3 cut(s) 37, 253, 592
Bsp143I GATC 2 cut(s) 86, 398
Bsp19I CCATGG 2 cut(s) 18, 67
BspANI GGCC 9 cut(s) 23, 66, 127, 170, 292, 306, 324, 436, 583
BspCNI CTCAG 3 cut(s) 166, 188, 197
BspLI GGNNCC 2 cut(s) 293, 336
BspPI GGATC 2 cut(s) 94, 406
BspTNI GGTCTC 1 cut(s) 196
BsrDI GCAATG 1 cut(s) 546
BsrI ACTGG 2 cut(s) 451, 658
BssMI GATC 2 cut(s) 86, 398
BssT1I CCWWGG 3 cut(s) 18, 67, 584
Bst2UI CCWGG 6 cut(s) 84, 117, 309, 321, 396, 441
Bst4CI ACNGT 2 cut(s) 491, 619
BstAPI GCANNNNNTGC 1 cut(s) 806
BstC8I GCNNGC 2 cut(s) 108, 645
BstDEI CTNAG 3 cut(s) 153, 175, 184
BstDSI CCRYGG 2 cut(s) 18, 67
BstENI CCTNNNNNAGG 1 cut(s) 393
BstF5I GGATG 3 cut(s) 10, 51, 489
BstKTI GATC 2 cut(s) 89, 401
BstMAI GTCTC 2 cut(s) 196, 369
BstMBI GATC 2 cut(s) 86, 398
BstMWI GCNNNNNNNGC 5 cut(s) 176, 341, 503, 770, 806
BstNI CCWGG 6 cut(s) 84, 117, 309, 321, 396, 441
BstNSI RCATGY 2 cut(s) 110, 715
BstSCI CCNGG 9 cut(s) 82, 115, 241, 293, 307, 319, 345, 394, 439
BstSLI GKGCMC 1 cut(s) 592
BstV1I GCAGC 2 cut(s) 90, 563
BstV2I GAAGAC 2 cut(s) 69, 366
BsuRI GGCC 9 cut(s) 23, 66, 127, 170, 292, 306, 324, 436, 583
BtgI CCRYGG 2 cut(s) 18, 67
BtsCI GGATG 3 cut(s) 10, 51, 489
BtsI GCAGTG 2 cut(s) 135, 632
BtsIMutI CAGTG 3 cut(s) 135, 632, 667
Cac8I GCNNGC 2 cut(s) 108, 645
Cfr13I GGNCC 5 cut(s) 126, 168, 291, 305, 323
CseI GACGC 1 cut(s) 626
CviAII CATG 6 cut(s) 19, 68, 107, 218, 518, 712
DdeI CTNAG 3 cut(s) 153, 175, 184
DpnI GATC 2 cut(s) 88, 400
DpnII GATC 2 cut(s) 86, 398
EaeI YGGCCR 1 cut(s) 21
Ecl136II GAGCTC 1 cut(s) 251
Eco130I CCWWGG 3 cut(s) 18, 67, 584
Eco24I GRGCYC 1 cut(s) 253
Eco31I GGTCTC 1 cut(s) 196
Eco53kI GAGCTC 1 cut(s) 251
Eco57I CTGAAG 1 cut(s) 609
EcoICRI GAGCTC 1 cut(s) 251
EcoNI CCTNNNNNAGG 1 cut(s) 393
EcoO109I RGGNCCY 2 cut(s) 168, 291
EcoRII CCWGG 6 cut(s) 82, 115, 307, 319, 394, 439
EcoT14I CCWWGG 3 cut(s) 18, 67, 584
EcoT22I ATGCAT 1 cut(s) 599
EcoT38I GRGCYC 1 cut(s) 253
ErhI CCWWGG 3 cut(s) 18, 67, 584
FaeI CATG 6 cut(s) 22, 71, 110, 221, 521, 715
FaqI GGGAC 1 cut(s) 457
FatI CATG 6 cut(s) 18, 67, 106, 217, 517, 711
FauNDI CATATG 1 cut(s) 670
Fnu4HI GCNGC 2 cut(s) 104, 552
FokI GGATG 3 cut(s) 17, 38, 496
FriOI GRGCYC 1 cut(s) 253
Fsp4HI GCNGC 2 cut(s) 104, 552
GluI GCNGC 2 cut(s) 104, 552
GsaI CCCAGC 1 cut(s) 102
GsuI CTGGAG 2 cut(s) 378, 558
HaeIII GGCC 9 cut(s) 23, 66, 127, 170, 292, 306, 324, 436, 583
HapII CCGG 4 cut(s) 243, 261, 295, 347
HgaI GACGC 1 cut(s) 626
Hin1II CATG 6 cut(s) 22, 71, 110, 221, 521, 715
HindIII AAGCTT 1 cut(s) 39
HinfI GANTC 1 cut(s) 412
HpaII CCGG 4 cut(s) 243, 261, 295, 347
HphI GGTGA 3 cut(s) 275, 399, 517
Hpy188I TCNGA 1 cut(s) 417
Hpy188III TCNNGA 4 cut(s) 201, 274, 338, 606
HpyAV CCTTC 4 cut(s) 204, 264, 426, 731
HpyCH4III ACNGT 2 cut(s) 491, 619
HpyCH4V TGCA 9 cut(s) 106, 110, 512, 551, 597, 647, 764, 795, 809
HpyF10VI GCNNNNNNNGC 5 cut(s) 176, 341, 503, 770, 806
HpyF3I CTNAG 3 cut(s) 153, 175, 184
Hsp92II CATG 6 cut(s) 22, 71, 110, 221, 521, 715
Kzo9I GATC 2 cut(s) 86, 398
LmnI GCTCC 4 cut(s) 248, 256, 322, 340
Lsp1109I GCAGC 2 cut(s) 90, 563
LweI GCATC 4 cut(s) 237, 331, 606, 656
MaeIII GTNAC 1 cut(s) 664
MalI GATC 2 cut(s) 88, 400
MboI GATC 2 cut(s) 86, 398
MboII GAAGA 7 cut(s) 69, 189, 249, 289, 366, 615, 776
MfeI CAATTG 1 cut(s) 461
MhlI GDGCHC 3 cut(s) 37, 253, 592
MlsI TGGCCA 1 cut(s) 23
MluCI AATT 6 cut(s) 208, 461, 557, 699, 750, 758
MluNI TGGCCA 1 cut(s) 23
MlyI GAGTC 1 cut(s) 406
MmeI TCCRAC 1 cut(s) 710
Mox20I TGGCCA 1 cut(s) 23
Mph1103I ATGCAT 1 cut(s) 599
MscI TGGCCA 1 cut(s) 23
MseI TTAA 2 cut(s) 698, 738
Msp20I TGGCCA 1 cut(s) 23
MspI CCGG 4 cut(s) 243, 261, 295, 347
MspR9I CCNGG 9 cut(s) 84, 117, 243, 295, 309, 321, 347, 396, 441
MunI CAATTG 1 cut(s) 461
Mva1269I GAATGC 1 cut(s) 179
MvaI CCWGG 6 cut(s) 84, 117, 309, 321, 396, 441
MwoI GCNNNNNNNGC 5 cut(s) 176, 341, 503, 770, 806
NciI CCSGG 3 cut(s) 243, 295, 347
NcoI CCATGG 2 cut(s) 18, 67
NdeI CATATG 1 cut(s) 670
NdeII GATC 2 cut(s) 86, 398
NlaIII CATG 6 cut(s) 22, 71, 110, 221, 521, 715
NlaIV GGNNCC 2 cut(s) 293, 336
NmeAIII GCCGAG 1 cut(s) 311
NmuCI GTSAC 1 cut(s) 664
NsiI ATGCAT 1 cut(s) 599
NspI RCATGY 2 cut(s) 110, 715
PaeI GCATGC 1 cut(s) 110
PasI CCCWGGG 1 cut(s) 116
PctI GAATGC 1 cut(s) 179
PfoI TCCNGGA 3 cut(s) 82, 241, 394
PkrI GCNGC 2 cut(s) 105, 553
PleI GAGTC 1 cut(s) 406
PpsI GAGTC 1 cut(s) 406
Psp124BI GAGCTC 1 cut(s) 253
Psp6I CCWGG 6 cut(s) 82, 115, 307, 319, 394, 439
PspFI CCCAGC 1 cut(s) 98
PspGI CCWGG 6 cut(s) 82, 115, 307, 319, 394, 439
PspN4I GGNNCC 2 cut(s) 293, 336
PspPI GGNCC 5 cut(s) 126, 168, 291, 305, 323
SacI GAGCTC 1 cut(s) 253
SaqAI TTAA 2 cut(s) 698, 738
SatI GCNGC 2 cut(s) 104, 552
Sau3AI GATC 2 cut(s) 86, 398
Sau96I GGNCC 5 cut(s) 126, 168, 291, 305, 323
SchI GAGTC 1 cut(s) 406
ScrFI CCNGG 9 cut(s) 84, 117, 243, 295, 309, 321, 347, 396, 441
SduI GDGCHC 3 cut(s) 37, 253, 592
SetI ASST 8 cut(s) 43, 154, 253, 407, 442, 538, 678, 775
SfaNI GCATC 4 cut(s) 237, 331, 606, 656
SmlI CTYRAG 1 cut(s) 36
SmoI CTYRAG 1 cut(s) 36
SphI GCATGC 1 cut(s) 110
Sse9I AATT 6 cut(s) 208, 461, 557, 699, 750, 758
SstI GAGCTC 1 cut(s) 253
StyD4I CCNGG 9 cut(s) 82, 115, 241, 293, 307, 319, 345, 394, 439
StyI CCWWGG 3 cut(s) 18, 67, 584
TaaI ACNGT 2 cut(s) 491, 619
TaqI TCGA 1 cut(s) 200
TasI AATT 6 cut(s) 208, 461, 557, 699, 750, 758
Tru1I TTAA 2 cut(s) 698, 738
Tru9I TTAA 2 cut(s) 698, 738
TscAI CASTG 3 cut(s) 142, 639, 667
TseFI GTSAC 1 cut(s) 664
TseI GCWGC 2 cut(s) 103, 551
Tsp45I GTSAC 1 cut(s) 664
TspDTI ATGAA 3 cut(s) 33, 500, 693
TspRI CASTG 3 cut(s) 142, 639, 667
XagI CCTNNNNNAGG 1 cut(s) 393
XapI RAATTY 2 cut(s) 750, 758
XceI RCATGY 2 cut(s) 110, 715
Zsp2I ATGCAT 1 cut(s) 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.