MD06G1081700.v1.1

DNA-binding protein ESCAROLA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0005616
Physical Location & Seq
Reverse (-)
19990893 .. 19996469
5577 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1081700.v1.1.491

Sequence Viewer

Length: 1050 bp
ATGGATGGGAGGGAAGCAATGGGATTGTCTGGTGGGTCAGCTTCATACTACATCAGTAGGGGAGGAGGGGTTGGTGGGTCTATACCTGGATCACAGGCTGGCGGCGGTGGCGGACTTCACCATGCTGCACTTGGGTTCAGGTCCATCTCAAACACCCTCATCCAACCTCAGTCCAATGTGAGGGTCAGCTCTGTGGGATCAACGTTCTCAGTGGAGCCTTCAAGACCCAATTTTGAACATCATGGCAGTACCATGAATGTCACTCCCGGAGCGCCTTCAGCTCAGCCTGTAAAGAAGAAAAGAGGGAGGCCCCGGAAGTATGTCCCCGATGGGTCAGTTTCTCTGGGGTTGTCTCCCATGTCGGCAACACCTACTCCTACACCGGGTTCAACCAGTCCGACCCCGAAACGTAGTAGAGGGAGGCCACCTGGGAGTGGTAGGAAGCAACAGTTGGCTGCTCTTGGAGATTGGATGAACACTTCGGCTGGATTAGCTTTTGCACCCCATGTTATCACCATTGGAGTTGGAGAGGACATTGCAGCAAAATTATTGTTATTCGCACAACAGAGACCAAGGGCGCTATGCATATTGTCTGGAAATGGTGCAGTGTCTTCTGTAACTCTACGCGAGCCTGCATCTACTGGTGTCAGTGTCACATATGAGGGTCGGTTCCAGATATTATGCTTGTCAGGTTCGTACTTAGTTGCCGAGGATGGTGGACCTCGCAATCGAACAGGGGGAATAAGTGTTTCCCTTTCTAATCCTAATGGTCATGTCATTGGTGGTGCTGTCGCAGTGCTTATTGCAGCAACTCCAGTGCAGGTTGTGCTTTGCAGTTTTGTATATGGTAGCTCAAAGACCAAGAACAAGCAAGTGGCTGGTCCCAATAGCGATGAGAATTCCGAGCCACAGCATAGCGAGAAATTGGCTCTACCAAGCAGTGCCCCACCTACTCAAAATTATAATCCCTCCGGTGCAGGCATTTGGCCTGGATCGCGGCAAGTTGATTTGAGAAATGGCCACACTGGTATTGACTTGACTCGCGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0043621
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

350

Amino Acids

35.72

Weight (kDa)

10.09

Isoelectric Point (pI)

40.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 168 - 280 1.5e-22 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 963
Acc36I ACCTGC 1 cut(s) 811
AccII CGCG 3 cut(s) 627, 997, 1044
AciI CCGC 5 cut(s) 102, 105, 111, 997, 1044
AclI AACGTT 1 cut(s) 203
AclWI GGATC 3 cut(s) 97, 205, 1000
AcoI YGGCCR 1 cut(s) 1018
AcsI RAATTY 1 cut(s) 898
AcuI CTGAAG 1 cut(s) 261
AfaI GTAC 2 cut(s) 250, 698
AfiI CCNNNNNNNGG 3 cut(s) 180, 383, 434
AgsI TTSAA 3 cut(s) 222, 236, 390
AjnI CCWGG 3 cut(s) 85, 427, 988
AjuI GAANNNNNNNTTGG 2 cut(s) 434, 466
AluBI AGCT 5 cut(s) 41, 189, 281, 494, 852
AluI AGCT 5 cut(s) 41, 189, 281, 494, 852
Alw26I GTCTC 2 cut(s) 357, 562
AlwI GGATC 3 cut(s) 97, 205, 1000
AoxI GGCC 4 cut(s) 308, 422, 986, 1018
ApeKI GCWGC 4 cut(s) 125, 455, 539, 806
ApoI RAATTY 1 cut(s) 898
AspLEI GCGC 2 cut(s) 274, 580
AspS9I GGNCC 4 cut(s) 141, 309, 719, 881
AsuC2I CCSGG 3 cut(s) 267, 313, 384
AsuHPI GGTGA 2 cut(s) 110, 505
AvaII GGWCC 3 cut(s) 141, 719, 881
BaeGI GKGCMC 1 cut(s) 946
BalI TGGCCA 1 cut(s) 1020
BbsI GAAGAC 1 cut(s) 603
BbvI GCAGC 4 cut(s) 112, 442, 551, 818
BccI CCATC 3 cut(s) 152, 323, 707
BciT130I CCWGG 3 cut(s) 87, 429, 990
BcnI CCSGG 3 cut(s) 267, 313, 384
BcoDI GTCTC 2 cut(s) 357, 562
BfoI RGCGCY 2 cut(s) 275, 581
BfuAI ACCTGC 1 cut(s) 811
BisI GCNGC 6 cut(s) 103, 126, 456, 540, 807, 998
BlpI GCTNAGC 1 cut(s) 282
BlsI GCNGC 6 cut(s) 104, 127, 457, 541, 808, 999
Bme1390I CCNGG 6 cut(s) 87, 267, 313, 384, 429, 990
Bme18I GGWCC 3 cut(s) 141, 719, 881
BmgT120I GGNCC 4 cut(s) 141, 309, 719, 881
BmiI GGNNCC 4 cut(s) 216, 311, 671, 883
BmrFI CCNGG 6 cut(s) 87, 267, 313, 384, 429, 990
BmsI GCATC 1 cut(s) 644
BpiI GAAGAC 1 cut(s) 603
BpmI CTGGAG 1 cut(s) 798
Bpu1102I GCTNAGC 1 cut(s) 282
BpuMI CCSGG 3 cut(s) 267, 313, 384
BsaI GGTCTC 1 cut(s) 562
BsaJI CCNNGG 4 cut(s) 311, 428, 572, 708
BsaWI WCCGGW 1 cut(s) 971
BsaXI ACNNNNNCTCC 4 cut(s) 54, 84, 358, 388
Bsc4I CCNNNNNNNGG 3 cut(s) 180, 383, 434
Bse1I ACTGG 4 cut(s) 393, 646, 815, 1030
Bse3DI GCAATG 2 cut(s) 24, 534
BseBI CCWGG 3 cut(s) 87, 429, 990
BseDI CCNNGG 4 cut(s) 311, 428, 572, 708
BseGI GGATG 4 cut(s) 10, 159, 477, 718
BseLI CCNNNNNNNGG 3 cut(s) 180, 383, 434
BseMI GCAATG 2 cut(s) 24, 534
BseMII CTCAG 3 cut(s) 182, 222, 296
BseNI ACTGG 4 cut(s) 393, 646, 815, 1030
BseRI GAGGAG 1 cut(s) 78
BseSI GKGCMC 1 cut(s) 946
BseXI GCAGC 4 cut(s) 112, 442, 551, 818
BsgI GTGCAG 4 cut(s) 111, 624, 839, 996
Bsh1236I CGCG 3 cut(s) 627, 997, 1044
BshFI GGCC 4 cut(s) 310, 424, 988, 1020
BsiSI CCGG 4 cut(s) 267, 313, 383, 972
BslFI GGGAC 2 cut(s) 308, 867
BslI CCNNNNNNNGG 3 cut(s) 180, 383, 434
BsmAI GTCTC 2 cut(s) 357, 562
BsmFI GGGAC 2 cut(s) 308, 867
BsnI GGCC 4 cut(s) 310, 424, 988, 1020
Bso31I GGTCTC 1 cut(s) 562
Bsp1286I GDGCHC 1 cut(s) 946
Bsp143I GATC 3 cut(s) 89, 197, 992
Bsp1720I GCTNAGC 1 cut(s) 282
BspACI CCGC 5 cut(s) 102, 105, 111, 997, 1044
BspANI GGCC 4 cut(s) 310, 424, 988, 1020
BspCNI CTCAG 3 cut(s) 181, 221, 295
BspFNI CGCG 3 cut(s) 627, 997, 1044
BspLI GGNNCC 4 cut(s) 216, 311, 671, 883
BspMI ACCTGC 1 cut(s) 811
BspPI GGATC 3 cut(s) 97, 205, 1000
BspTNI GGTCTC 1 cut(s) 562
BsrDI GCAATG 2 cut(s) 24, 534
BsrI ACTGG 4 cut(s) 393, 646, 815, 1030
BssECI CCNNGG 4 cut(s) 311, 428, 572, 708
BssMI GATC 3 cut(s) 89, 197, 992
BssT1I CCWWGG 1 cut(s) 572
Bst2UI CCWGG 3 cut(s) 87, 429, 990
Bst4CI ACNGT 1 cut(s) 450
BstAPI GCANNNNNTGC 1 cut(s) 826
BstC8I GCNNGC 4 cut(s) 100, 629, 633, 979
BstDEI CTNAG 4 cut(s) 168, 208, 282, 700
BstF5I GGATG 4 cut(s) 10, 159, 477, 718
BstFNI CGCG 3 cut(s) 627, 997, 1044
BstH2I RGCGCY 2 cut(s) 275, 581
BstHHI GCGC 2 cut(s) 274, 580
BstKTI GATC 3 cut(s) 92, 200, 995
BstMAI GTCTC 2 cut(s) 357, 562
BstMBI GATC 3 cut(s) 89, 197, 992
BstMWI GCNNNNNNNGC 5 cut(s) 108, 278, 491, 826, 994
BstNI CCWGG 3 cut(s) 87, 429, 990
BstSCI CCNGG 6 cut(s) 85, 265, 311, 382, 427, 988
BstSLI GKGCMC 1 cut(s) 946
BstUI CGCG 3 cut(s) 627, 997, 1044
BstV1I GCAGC 4 cut(s) 112, 442, 551, 818
BstV2I GAAGAC 1 cut(s) 603
BsuRI GGCC 4 cut(s) 310, 424, 988, 1020
BtgZI GCGATG 1 cut(s) 906
BtsCI GGATG 4 cut(s) 10, 159, 477, 718
BtsI GCAGTG 3 cut(s) 612, 801, 946
BtsIMutI CAGTG 7 cut(s) 216, 612, 655, 801, 822, 946, 1023
BveI ACCTGC 1 cut(s) 811
Cac8I GCNNGC 4 cut(s) 100, 629, 633, 979
CfoI GCGC 2 cut(s) 274, 580
Cfr13I GGNCC 4 cut(s) 141, 309, 719, 881
Csp6I GTAC 2 cut(s) 249, 697
CviAII CATG 6 cut(s) 122, 242, 253, 358, 506, 773
CviQI GTAC 2 cut(s) 249, 697
DdeI CTNAG 4 cut(s) 168, 208, 282, 700
DpnI GATC 3 cut(s) 91, 199, 994
DpnII GATC 3 cut(s) 89, 197, 992
EaeI YGGCCR 1 cut(s) 1018
EciI GGCGGA 1 cut(s) 126
Eco130I CCWWGG 1 cut(s) 572
Eco31I GGTCTC 1 cut(s) 562
Eco47I GGWCC 3 cut(s) 141, 719, 881
Eco57I CTGAAG 1 cut(s) 261
EcoO109I RGGNCCY 1 cut(s) 309
EcoRI GAATTC 1 cut(s) 898
EcoRII CCWGG 3 cut(s) 85, 427, 988
EcoT14I CCWWGG 1 cut(s) 572
EcoT22I ATGCAT 1 cut(s) 587
ErhI CCWWGG 1 cut(s) 572
FaeI CATG 6 cut(s) 125, 245, 256, 361, 509, 776
FaqI GGGAC 2 cut(s) 308, 867
FatI CATG 6 cut(s) 121, 241, 252, 357, 505, 772
FauNDI CATATG 1 cut(s) 658
Fnu4HI GCNGC 6 cut(s) 103, 126, 456, 540, 807, 998
FokI GGATG 4 cut(s) 17, 146, 484, 725
Fsp4HI GCNGC 6 cut(s) 103, 126, 456, 540, 807, 998
GlaI GCGC 2 cut(s) 273, 579
GluI GCNGC 6 cut(s) 103, 126, 456, 540, 807, 998
GsuI CTGGAG 1 cut(s) 798
HaeII RGCGCY 2 cut(s) 275, 581
HaeIII GGCC 4 cut(s) 310, 424, 988, 1020
HapII CCGG 4 cut(s) 267, 313, 383, 972
HhaI GCGC 2 cut(s) 274, 580
Hin1II CATG 6 cut(s) 125, 245, 256, 361, 509, 776
Hin6I GCGC 2 cut(s) 272, 578
HinP1I GCGC 2 cut(s) 272, 578
HinfI GANTC 1 cut(s) 1039
HpaII CCGG 4 cut(s) 267, 313, 383, 972
HphI GGTGA 2 cut(s) 110, 505
Hpy166II GTNNAC 1 cut(s) 719
Hpy188I TCNGA 2 cut(s) 399, 904
Hpy188III TCNNGA 3 cut(s) 222, 594, 673
Hpy8I GTNNAC 1 cut(s) 719
HpyAV CCTTC 2 cut(s) 228, 285
HpyCH4III ACNGT 1 cut(s) 450
HpyCH4IV ACGT 2 cut(s) 203, 409
HpyF10VI GCNNNNNNNGC 5 cut(s) 108, 278, 491, 826, 994
HpyF3I CTNAG 4 cut(s) 168, 208, 282, 700
HpySE526I ACGT 2 cut(s) 203, 409
Hsp92II CATG 6 cut(s) 125, 245, 256, 361, 509, 776
HspAI GCGC 2 cut(s) 272, 578
Kzo9I GATC 3 cut(s) 89, 197, 992
LmnI GCTCC 2 cut(s) 214, 269
Lsp1109I GCAGC 4 cut(s) 112, 442, 551, 818
LweI GCATC 1 cut(s) 644
MaeII ACGT 2 cut(s) 203, 409
MaeIII GTNAC 3 cut(s) 259, 616, 652
MalI GATC 3 cut(s) 91, 199, 994
MboI GATC 3 cut(s) 89, 197, 992
MboII GAAGA 2 cut(s) 307, 603
MhlI GDGCHC 1 cut(s) 946
MlsI TGGCCA 1 cut(s) 1020
MluCI AATT 5 cut(s) 229, 545, 898, 923, 958
MluNI TGGCCA 1 cut(s) 1020
MlyI GAGTC 1 cut(s) 1033
MmeI TCCRAC 3 cut(s) 187, 422, 505
Mox20I TGGCCA 1 cut(s) 1020
Mph1103I ATGCAT 1 cut(s) 587
MscI TGGCCA 1 cut(s) 1020
Msp20I TGGCCA 1 cut(s) 1020
MspI CCGG 4 cut(s) 267, 313, 383, 972
MspR9I CCNGG 6 cut(s) 87, 267, 313, 384, 429, 990
MvaI CCWGG 3 cut(s) 87, 429, 990
MvnI CGCG 3 cut(s) 627, 997, 1044
MwoI GCNNNNNNNGC 5 cut(s) 108, 278, 491, 826, 994
NciI CCSGG 3 cut(s) 267, 313, 384
NdeI CATATG 1 cut(s) 658
NdeII GATC 3 cut(s) 89, 197, 992
NlaIII CATG 6 cut(s) 125, 245, 256, 361, 509, 776
NlaIV GGNNCC 4 cut(s) 216, 311, 671, 883
NmeAIII GCCGAG 1 cut(s) 733
NmuCI GTSAC 2 cut(s) 259, 652
NsiI ATGCAT 1 cut(s) 587
PfoI TCCNGGA 1 cut(s) 265
PkrI GCNGC 6 cut(s) 104, 127, 457, 541, 808, 999
PleI GAGTC 1 cut(s) 1033
PpsI GAGTC 1 cut(s) 1033
PsiI TTATAA 1 cut(s) 963
Psp1406I AACGTT 1 cut(s) 203
Psp6I CCWGG 3 cut(s) 85, 427, 988
PspGI CCWGG 3 cut(s) 85, 427, 988
PspN4I GGNNCC 4 cut(s) 216, 311, 671, 883
PspPI GGNCC 4 cut(s) 141, 309, 719, 881
RsaI GTAC 2 cut(s) 250, 698
RsaNI GTAC 2 cut(s) 249, 697
SatI GCNGC 6 cut(s) 103, 126, 456, 540, 807, 998
Sau3AI GATC 3 cut(s) 89, 197, 992
Sau96I GGNCC 4 cut(s) 141, 309, 719, 881
SchI GAGTC 1 cut(s) 1033
ScrFI CCNGG 6 cut(s) 87, 267, 313, 384, 429, 990
SduI GDGCHC 1 cut(s) 946
SfaNI GCATC 1 cut(s) 644
SinI GGWCC 3 cut(s) 141, 719, 881
Sse9I AATT 5 cut(s) 229, 545, 898, 923, 958
SsiI CCGC 5 cut(s) 102, 105, 111, 997, 1044
StyD4I CCNGG 6 cut(s) 85, 265, 311, 382, 427, 988
StyI CCWWGG 1 cut(s) 572
TaaI ACNGT 1 cut(s) 450
TaiI ACGT 2 cut(s) 206, 412
TaqI TCGA 1 cut(s) 730
TasI AATT 5 cut(s) 229, 545, 898, 923, 958
TauI GCSGC 2 cut(s) 105, 1000
TscAI CASTG 7 cut(s) 216, 612, 655, 801, 822, 946, 1030
TseFI GTSAC 2 cut(s) 259, 652
TseI GCWGC 4 cut(s) 125, 455, 539, 806
Tsp45I GTSAC 2 cut(s) 259, 652
TspDTI ATGAA 3 cut(s) 33, 269, 488
TspRI CASTG 7 cut(s) 216, 612, 655, 801, 822, 946, 1030
VpaK11BI GGWCC 3 cut(s) 141, 719, 881
XapI RAATTY 1 cut(s) 898
XcmI CCANNNNNNNNNTGG 1 cut(s) 128
Zsp2I ATGCAT 1 cut(s) 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.