AT1G67330

Polysaccharide biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005005
Physical Location & Seq
Forward (+)
25213852 .. 25215174
1323 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G67330.1

Sequence Viewer

Length: 876 bp
ATGATTCAAGACAAGTCTAAAGGTGCAAAGCAAACGCTTCTAGAGCGGCCATGGTTCCTCGCTGTGGCTCTAGCTGGTCTTATAGGTGGCGCAATGCTCATCACAAGCTTCATCCGAGCTACGGACAACACCTTGTCACTCTGCTCCACGGCTAAGAACACAGCTGCGTCTATAGCCAAATACACAGCCACCCCAATCCAACTCCAATCCATCGTCCACTACGCCACTTCACACACCGTCCCTCAACAATCTTTCGAGGAGATCTCGATCTCTTTAAACGTCCTCAAGGAGCGTCTCCCTTGTAACTTTCTAGTCTTTGGCCTCGGCCGCGACTCCCTCATGTGGGCCTCCCTCAATCCAGGTGGCACAACTGTGTTCTTGGAAGAGGATCCTGAGTGGATAGAGGCCGTCCTCAAGGACGCCCCATCCCTCAGGGCCCACCATGTTCAGTACCGGACCCACCTTTCTGAGGCCGGCCGCCTTCTCTCGACTTACAAGAACGAACCCATGTGTTTACCAGCTAAAGCTTTCCCGATCCGCTACAACGAAAAGTGTCCCTTGGCGTTGACTTCTCTCCCTGATGAGTTCTATGATACCGAGTGGGATCTGATCATGGTGGACGCACCAAAAGGGTACTTCCCAGAGGCGCCAGGAAGGATGGCGGCGATATTTTCCTCGGCCATCATGGCACGTAACCGGAAAGGTGATGGCACGACTCACGTCTTCCTTCATGACGTTAACCGCAAAGTGGAGAACGCTTTTGCCAATGAGTTCCTTTGTGAGAAGTATAAGGTCAACTCCGTAGGTAGGCTCTGGCACTTCGAGATACCTAACGCCGCTAACATGACCGACCAGCCTGGTGACCGGTTTTGCTAG

Protein Analysis

291

Amino Acids

32.47

Weight (kDa)

6.45

Isoelectric Point (pI)

33.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IRX15_IRX15L_GXM PF21729 86 - 274 1.6e-86 IRX15/IRX15L/GXM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 646
AccBSI CCGCTC 1 cut(s) 46
AccII CGCG 1 cut(s) 330
AciI CCGC 7 cut(s) 46, 328, 478, 538, 662, 742, 837
AclWI GGATC 4 cut(s) 383, 396, 529, 612
AcoI YGGCCR 4 cut(s) 47, 325, 475, 678
AcyI GRCGYC 2 cut(s) 420, 647
AfaI GTAC 2 cut(s) 452, 635
AfiI CCNNNNNNNGG 7 cut(s) 64, 121, 342, 343, 469, 748, 807
AgeI ACCGGT 1 cut(s) 864
AgsI TTSAA 1 cut(s) 8
AjiI CACGTC 1 cut(s) 721
AjnI CCWGG 3 cut(s) 358, 649, 856
AleI CACNNNNGTG 1 cut(s) 371
AluBI AGCT 6 cut(s) 74, 108, 119, 164, 521, 527
AluI AGCT 6 cut(s) 74, 108, 119, 164, 521, 527
Alw26I GTCTC 1 cut(s) 299
AlwI GGATC 4 cut(s) 383, 396, 529, 612
AoxI GGCC 9 cut(s) 47, 319, 325, 345, 405, 435, 471, 475, 678
ApaI GGGCCC 1 cut(s) 439
ApeKI GCWGC 1 cut(s) 164
AsiGI ACCGGT 1 cut(s) 864
AspLEI GCGC 2 cut(s) 92, 649
AspS9I GGNCC 4 cut(s) 345, 435, 436, 456
AsuHPI GGTGA 2 cut(s) 716, 872
AvaII GGWCC 1 cut(s) 456
AxyI CCTNAGG 1 cut(s) 431
BaeGI GKGCMC 1 cut(s) 439
BamHI GGATCC 1 cut(s) 388
BanI GGYRCC 1 cut(s) 646
BanII GRGCYC 1 cut(s) 439
BbsI GAAGAC 1 cut(s) 715
BbvI GCAGC 1 cut(s) 151
BccI CCATC 5 cut(s) 218, 433, 652, 689, 701
BceAI ACGGC 2 cut(s) 165, 392
BciT130I CCWGG 3 cut(s) 360, 651, 858
BclI TGATCA 1 cut(s) 609
BcoDI GTCTC 1 cut(s) 299
BfaI CTAG 4 cut(s) 41, 71, 311, 874
BfmI CTRYAG 1 cut(s) 171
BfoI RGCGCY 1 cut(s) 650
BglI GCCNNNNNGGC 1 cut(s) 686
BglII AGATCT 1 cut(s) 261
BisI GCNGC 6 cut(s) 47, 165, 328, 478, 663, 837
BlsI GCNGC 6 cut(s) 48, 166, 329, 479, 664, 838
Bme1390I CCNGG 3 cut(s) 360, 651, 858
Bme18I GGWCC 1 cut(s) 456
BmgBI CACGTC 1 cut(s) 721
BmgT120I GGNCC 4 cut(s) 345, 435, 436, 456
BmiI GGNNCC 5 cut(s) 56, 390, 437, 458, 648
BmrFI CCNGG 3 cut(s) 360, 651, 858
BoxI GACNNNNGTC 1 cut(s) 719
BpiI GAAGAC 1 cut(s) 715
BplI GAGNNNNNCTC 2 cut(s) 248, 280
BpuEI CTTGAG 2 cut(s) 269, 398
BsaAI YACGTR 1 cut(s) 692
BsaBI GATNNNNATC 1 cut(s) 266
BsaHI GRCGYC 2 cut(s) 420, 647
BsaJI CCNNGG 5 cut(s) 50, 147, 322, 558, 675
BsaWI WCCGGW 3 cut(s) 453, 696, 864
Bsc4I CCNNNNNNNGG 7 cut(s) 64, 121, 342, 343, 469, 748, 807
Bse118I RCCGGY 2 cut(s) 473, 864
Bse21I CCTNAGG 1 cut(s) 431
Bse3DI GCAATG 1 cut(s) 99
Bse8I GATNNNNATC 1 cut(s) 266
BseBI CCWGG 3 cut(s) 360, 651, 858
BseDI CCNNGG 5 cut(s) 50, 147, 322, 558, 675
BseGI GGATG 3 cut(s) 111, 425, 663
BseJI GATNNNNATC 1 cut(s) 266
BseLI CCNNNNNNNGG 7 cut(s) 64, 121, 342, 343, 469, 748, 807
BseMI GCAATG 1 cut(s) 99
BseMII CTCAG 3 cut(s) 384, 445, 459
BseRI GAGGAG 1 cut(s) 272
BseSI GKGCMC 1 cut(s) 439
BseX3I CGGCCG 2 cut(s) 325, 475
BseXI GCAGC 1 cut(s) 151
Bsh1236I CGCG 1 cut(s) 330
Bsh1285I CGRYCG 2 cut(s) 328, 478
BshFI GGCC 9 cut(s) 49, 321, 327, 347, 407, 437, 473, 477, 680
BshNI GGYRCC 1 cut(s) 646
BshTI ACCGGT 1 cut(s) 864
BsiEI CGRYCG 2 cut(s) 328, 478
BsiSI CCGG 4 cut(s) 454, 474, 697, 865
BslFI GGGAC 2 cut(s) 224, 540
BslI CCNNNNNNNGG 7 cut(s) 64, 121, 342, 343, 469, 748, 807
BsmAI GTCTC 1 cut(s) 299
BsmBI CGTCTC 1 cut(s) 299
BsmFI GGGAC 2 cut(s) 224, 540
BsnI GGCC 9 cut(s) 49, 321, 327, 347, 407, 437, 473, 477, 680
Bsp120I GGGCCC 1 cut(s) 435
Bsp1286I GDGCHC 1 cut(s) 439
Bsp143I GATC 6 cut(s) 261, 267, 388, 534, 604, 609
Bsp19I CCATGG 1 cut(s) 50
BspACI CCGC 7 cut(s) 46, 328, 478, 538, 662, 742, 837
BspANI GGCC 9 cut(s) 49, 321, 327, 347, 407, 437, 473, 477, 680
BspCNI CTCAG 3 cut(s) 385, 444, 460
BspFNI CGCG 1 cut(s) 330
BspHI TCATGA 1 cut(s) 730
BspLI GGNNCC 5 cut(s) 56, 390, 437, 458, 648
BspPI GGATC 4 cut(s) 383, 396, 529, 612
BspT107I GGYRCC 1 cut(s) 646
BsrBI CCGCTC 1 cut(s) 46
BsrDI GCAATG 1 cut(s) 99
BsrFI RCCGGY 2 cut(s) 473, 864
BssAI RCCGGY 2 cut(s) 473, 864
BssECI CCNNGG 5 cut(s) 50, 147, 322, 558, 675
BssMI GATC 6 cut(s) 261, 267, 388, 534, 604, 609
BssNI GRCGYC 2 cut(s) 420, 647
BssT1I CCWWGG 2 cut(s) 50, 558
Bst2UI CCWGG 3 cut(s) 360, 651, 858
Bst4CI ACNGT 2 cut(s) 238, 373
Bst6I CTCTTC 1 cut(s) 378
BstACI GRCGYC 2 cut(s) 420, 647
BstBAI YACGTR 1 cut(s) 692
BstC8I GCNNGC 1 cut(s) 475
BstDEI CTNAG 4 cut(s) 153, 393, 431, 468
BstDSI CCRYGG 2 cut(s) 50, 147
BstEII GGTNACC 1 cut(s) 860
BstENI CCTNNNNNAGG 1 cut(s) 467
BstF5I GGATG 3 cut(s) 111, 425, 663
BstFNI CGCG 1 cut(s) 330
BstH2I RGCGCY 1 cut(s) 650
BstHHI GCGC 2 cut(s) 92, 649
BstKTI GATC 6 cut(s) 264, 270, 391, 537, 607, 612
BstMAI GTCTC 1 cut(s) 299
BstMBI GATC 6 cut(s) 261, 267, 388, 534, 604, 609
BstMCI CGRYCG 2 cut(s) 328, 478
BstMWI GCNNNNNNNGC 4 cut(s) 43, 173, 327, 686
BstNI CCWGG 3 cut(s) 360, 651, 858
BstPAI GACNNNNGTC 1 cut(s) 719
BstPI GGTNACC 1 cut(s) 860
BstSCI CCNGG 3 cut(s) 358, 649, 856
BstSFI CTRYAG 1 cut(s) 171
BstSLI GKGCMC 1 cut(s) 439
BstUI CGCG 1 cut(s) 330
BstV1I GCAGC 1 cut(s) 151
BstV2I GAAGAC 1 cut(s) 715
BstX2I RGATCY 3 cut(s) 261, 388, 604
BstYI RGATCY 3 cut(s) 261, 388, 604
BstZI CGGCCG 2 cut(s) 325, 475
Bsu36I CCTNAGG 1 cut(s) 431
BsuRI GGCC 9 cut(s) 49, 321, 327, 347, 407, 437, 473, 477, 680
BtgI CCRYGG 2 cut(s) 50, 147
BtrI CACGTC 1 cut(s) 721
BtsCI GGATG 3 cut(s) 111, 425, 663
Cac8I GCNNGC 1 cut(s) 475
CciI TCATGA 1 cut(s) 730
CfoI GCGC 2 cut(s) 92, 649
Cfr10I RCCGGY 2 cut(s) 473, 864
Cfr13I GGNCC 4 cut(s) 345, 435, 436, 456
CseI GACGC 4 cut(s) 156, 281, 428, 629
Csp6I GTAC 2 cut(s) 451, 634
CspAI ACCGGT 1 cut(s) 864
CspCI CAANNNNNGTGG 2 cut(s) 343, 378
CviAII CATG 8 cut(s) 51, 340, 443, 508, 613, 685, 731, 844
CviQI GTAC 2 cut(s) 451, 634
DdeI CTNAG 4 cut(s) 153, 393, 431, 468
DinI GGCGCC 1 cut(s) 648
DpnI GATC 6 cut(s) 263, 269, 390, 536, 606, 611
DpnII GATC 6 cut(s) 261, 267, 388, 534, 604, 609
DraI TTTAAA 1 cut(s) 276
EaeI YGGCCR 4 cut(s) 47, 325, 475, 678
EagI CGGCCG 2 cut(s) 325, 475
Eam1104I CTCTTC 1 cut(s) 378
EarI CTCTTC 1 cut(s) 378
EclXI CGGCCG 2 cut(s) 325, 475
Eco130I CCWWGG 2 cut(s) 50, 558
Eco24I GRGCYC 1 cut(s) 439
Eco47I GGWCC 1 cut(s) 456
Eco52I CGGCCG 2 cut(s) 325, 475
Eco81I CCTNAGG 1 cut(s) 431
Eco91I GGTNACC 1 cut(s) 860
EcoNI CCTNNNNNAGG 1 cut(s) 467
EcoO109I RGGNCCY 1 cut(s) 435
EcoO65I GGTNACC 1 cut(s) 860
EcoRII CCWGG 3 cut(s) 358, 649, 856
EcoT14I CCWWGG 2 cut(s) 50, 558
EcoT38I GRGCYC 1 cut(s) 439
EgeI GGCGCC 1 cut(s) 648
EheI GGCGCC 1 cut(s) 648
ErhI CCWWGG 2 cut(s) 50, 558
Esp3I CGTCTC 1 cut(s) 299
FaeI CATG 8 cut(s) 54, 343, 446, 511, 616, 688, 734, 847
FalI AAGNNNNNCTT 2 cut(s) 542, 574
FaqI GGGAC 2 cut(s) 224, 540
FatI CATG 8 cut(s) 50, 339, 442, 507, 612, 684, 730, 843
FbaI TGATCA 1 cut(s) 609
Fnu4HI GCNGC 6 cut(s) 47, 165, 328, 478, 663, 837
FokI GGATG 3 cut(s) 98, 412, 670
FriOI GRGCYC 1 cut(s) 439
FseI GGCCGGCC 1 cut(s) 477
Fsp4HI GCNGC 6 cut(s) 47, 165, 328, 478, 663, 837
FspBI CTAG 4 cut(s) 41, 71, 311, 874
GlaI GCGC 2 cut(s) 91, 648
GluI GCNGC 6 cut(s) 47, 165, 328, 478, 663, 837
HaeII RGCGCY 1 cut(s) 650
HaeIII GGCC 9 cut(s) 49, 321, 327, 347, 407, 437, 473, 477, 680
HapII CCGG 4 cut(s) 454, 474, 697, 865
HgaI GACGC 4 cut(s) 156, 281, 428, 629
HhaI GCGC 2 cut(s) 92, 649
Hin1I GRCGYC 2 cut(s) 420, 647
Hin1II CATG 8 cut(s) 54, 343, 446, 511, 616, 688, 734, 847
Hin6I GCGC 2 cut(s) 90, 647
HinP1I GCGC 2 cut(s) 90, 647
HincII GTYRAC 3 cut(s) 567, 739, 796
HindII GTYRAC 3 cut(s) 567, 739, 796
HindIII AAGCTT 2 cut(s) 106, 525
HinfI GANTC 3 cut(s) 4, 332, 715
HpaI GTTAAC 1 cut(s) 739
HpaII CCGG 4 cut(s) 454, 474, 697, 865
HphI GGTGA 2 cut(s) 716, 872
Hpy166II GTNNAC 6 cut(s) 217, 515, 567, 619, 739, 796
Hpy188I TCNGA 3 cut(s) 116, 469, 609
Hpy188III TCNNGA 8 cut(s) 8, 41, 265, 392, 487, 532, 731, 823
Hpy8I GTNNAC 6 cut(s) 217, 515, 567, 619, 739, 796
HpyAV CCTTC 3 cut(s) 491, 648, 737
HpyCH4III ACNGT 2 cut(s) 238, 373
HpyCH4IV ACGT 4 cut(s) 279, 691, 720, 735
HpyCH4V TGCA 1 cut(s) 26
HpyF10VI GCNNNNNNNGC 4 cut(s) 43, 173, 327, 686
HpyF3I CTNAG 4 cut(s) 153, 393, 431, 468
HpySE526I ACGT 4 cut(s) 279, 691, 720, 735
Hsp92I GRCGYC 2 cut(s) 420, 647
Hsp92II CATG 8 cut(s) 54, 343, 446, 511, 616, 688, 734, 847
HspAI GCGC 2 cut(s) 90, 647
KasI GGCGCC 1 cut(s) 646
KroI GCCGGC 1 cut(s) 473
KroNI GCCGGC 1 cut(s) 475
Ksp22I TGATCA 1 cut(s) 609
KspAI GTTAAC 1 cut(s) 739
Kzo9I GATC 6 cut(s) 261, 267, 388, 534, 604, 609
LmnI GCTCC 2 cut(s) 149, 289
Lsp1109I GCAGC 1 cut(s) 151
MaeI CTAG 4 cut(s) 41, 71, 311, 874
MaeII ACGT 4 cut(s) 279, 691, 720, 735
MaeIII GTNAC 4 cut(s) 135, 302, 692, 860
MalI GATC 6 cut(s) 263, 269, 390, 536, 606, 611
MbiI CCGCTC 1 cut(s) 46
MboI GATC 6 cut(s) 261, 267, 388, 534, 604, 609
MboII GAAGA 2 cut(s) 395, 715
MflI RGATCY 3 cut(s) 261, 388, 604
MhlI GDGCHC 1 cut(s) 439
Mly113I GGCGCC 1 cut(s) 647
MlyI GAGTC 2 cut(s) 326, 709
MmeI TCCRAC 1 cut(s) 223
MroNI GCCGGC 1 cut(s) 473
MseI TTAA 2 cut(s) 275, 738
MslI CAYNNNNRTG 1 cut(s) 371
MspA1I CMGCKG 1 cut(s) 164
MspI CCGG 4 cut(s) 454, 474, 697, 865
MspR9I CCNGG 3 cut(s) 360, 651, 858
MvaI CCWGG 3 cut(s) 360, 651, 858
MvnI CGCG 1 cut(s) 330
MwoI GCNNNNNNNGC 4 cut(s) 43, 173, 327, 686
NaeI GCCGGC 1 cut(s) 475
NarI GGCGCC 1 cut(s) 647
NcoI CCATGG 1 cut(s) 50
NdeII GATC 6 cut(s) 261, 267, 388, 534, 604, 609
NgoMIV GCCGGC 1 cut(s) 473
NlaIII CATG 8 cut(s) 54, 343, 446, 511, 616, 688, 734, 847
NlaIV GGNNCC 5 cut(s) 56, 390, 437, 458, 648
NmeAIII GCCGAG 2 cut(s) 303, 656
NmuCI GTSAC 2 cut(s) 135, 860
OliI CACNNNNGTG 1 cut(s) 371
PagI TCATGA 1 cut(s) 730
PdiI GCCGGC 1 cut(s) 475
PfeI GAWTC 1 cut(s) 4
PinAI ACCGGT 1 cut(s) 864
PkrI GCNGC 6 cut(s) 48, 166, 329, 479, 664, 838
PleI GAGTC 2 cut(s) 326, 709
PluTI GGCGCC 1 cut(s) 650
PpsI GAGTC 2 cut(s) 326, 709
Ppu21I YACGTR 1 cut(s) 692
PshAI GACNNNNGTC 1 cut(s) 719
Psp6I CCWGG 3 cut(s) 358, 649, 856
PspEI GGTNACC 1 cut(s) 860
PspGI CCWGG 3 cut(s) 358, 649, 856
PspN4I GGNNCC 5 cut(s) 56, 390, 437, 458, 648
PspOMI GGGCCC 1 cut(s) 435
PspPI GGNCC 4 cut(s) 345, 435, 436, 456
PsuI RGATCY 3 cut(s) 261, 388, 604
PvuII CAGCTG 1 cut(s) 164
RigI GGCCGGCC 1 cut(s) 477
RsaI GTAC 2 cut(s) 452, 635
RsaNI GTAC 2 cut(s) 451, 634
RseI CAYNNNNRTG 1 cut(s) 371
SaqAI TTAA 2 cut(s) 275, 738
SatI GCNGC 6 cut(s) 47, 165, 328, 478, 663, 837
Sau3AI GATC 6 cut(s) 261, 267, 388, 534, 604, 609
Sau96I GGNCC 4 cut(s) 345, 435, 436, 456
SchI GAGTC 2 cut(s) 326, 709
ScrFI CCNGG 3 cut(s) 360, 651, 858
SduI GDGCHC 1 cut(s) 439
SfcI CTRYAG 1 cut(s) 171
SfoI GGCGCC 1 cut(s) 648
SinI GGWCC 1 cut(s) 456
SmiMI CAYNNNNRTG 1 cut(s) 371
SmlI CTYRAG 2 cut(s) 284, 413
SmoI CTYRAG 2 cut(s) 284, 413
SsiI CCGC 7 cut(s) 46, 328, 478, 538, 662, 742, 837
SspDI GGCGCC 1 cut(s) 646
SspMI CTAG 4 cut(s) 41, 71, 311, 874
StyD4I CCNGG 3 cut(s) 358, 649, 856
StyI CCWWGG 2 cut(s) 50, 558
TaaI ACNGT 2 cut(s) 238, 373
TaiI ACGT 4 cut(s) 282, 694, 723, 738
TaqI TCGA 4 cut(s) 255, 266, 488, 822
TaqII GACCGA 1 cut(s) 863
TauI GCSGC 5 cut(s) 49, 330, 480, 665, 839
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 2 cut(s) 275, 738
Tru9I TTAA 2 cut(s) 275, 738
TseFI GTSAC 2 cut(s) 135, 860
TseI GCWGC 1 cut(s) 164
Tsp45I GTSAC 2 cut(s) 135, 860
TspDTI ATGAA 2 cut(s) 100, 719
TspGWI ACGGA 2 cut(s) 137, 790
VpaK11BI GGWCC 1 cut(s) 456
XagI CCTNNNNNAGG 1 cut(s) 467
XbaI TCTAGA 1 cut(s) 40
XspI CTAG 4 cut(s) 41, 71, 311, 874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.