FvH4_4g23700

Polysaccharide biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0005005
Physical Location & Seq
Forward (+)
26106047 .. 26107378
1332 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g23700.t1

Sequence Viewer

Length: 819 bp
ATGATGACTCGCACAAACCTAAACAGATTGTTAGCCGCCGTTTTGGTTATCTTCCTCGGCGCCGCCTTGTTCATCTCCAGCCTCACCGGAACAGGACCCTCCTTCCTCTGCTCTCTCCCTAATCCCATTGACGATTATCACCCCACGGCAACACAGCTCCAGGCGGTGCTCCACTACGCCACGTCATCTAAGACCCCGCAACAATCGCGAGCCGAGATCAGCGTCTCCTTCGACGTCCTTAAATCCAGGGCGCCCTGCAACTTCCTCGTCTTCGGTCTCGGCTACGACTCGCTCATGTGGGCCTCATTCAACCCACGTGGCACGACGCTGTTCCTCGAGGAGGACCCCAAGTGGGTCCAGACAGTCCTTAAGGACTCGCCGGAGCTAAGGGCCGAGACCGTTAACTACCGCACGCAGCTGAAGCAGGCGGACGAGCTGCTCTCCTCGTACAAATCCGAAGCGGCGTGCGCGCCGTCCGAGGCGGTGCTACGTGGCAACGAGAGGTGCAAGCTGGCGCTGAGCAACCTGCCGGAGGAGGTGTACGAGAAGGAGTGGGACTTGATCATGATCGACGCGCCAAGGGGTTACTTTCCCGAGGCGCCTGGTCGGATGGCGGTGATATTCTCGGCGGCGGTTATGGCTAGGAGGAGGCGGGGGTCAGGTGCGACGCACGTGTTTTTGCACGACGTGGACAGGAAGGTGGAGAAGGCTTTTGCCGAGGAGTTTCTCTGCCGGAAGTACCTTGTTAAGGCCGTCGGGAGGCTCTGGCATTTTGAGATTCCCTCGGCCACTAACGGCAGCGACGCACGGTTCTGTTAA

Protein Analysis

273

Amino Acids

30.39

Weight (kDa)

7.62

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IRX15_IRX15L_GXM PF21729 71 - 258 1e-83 IRX15/IRX15L/GXM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 237
AbsI CCTCGAGG 1 cut(s) 335
Acc36I ACCTGC 1 cut(s) 534
AccB1I GGYRCC 3 cut(s) 59, 250, 598
AccII CGCG 3 cut(s) 208, 470, 575
AcoI YGGCCR 1 cut(s) 786
AcuI CTGAAG 1 cut(s) 440
AcvI CACGTG 2 cut(s) 317, 673
AcyI GRCGYC 4 cut(s) 60, 234, 251, 599
AdeI CACNNNGTG 1 cut(s) 688
AfaI GTAC 3 cut(s) 449, 542, 740
AfiI CCNNNNNNNGG 5 cut(s) 340, 352, 532, 748, 759
AflII CTTAAG 1 cut(s) 368
AflIII ACRYGT 1 cut(s) 672
AgsI TTSAA 1 cut(s) 310
AjiI CACGTC 2 cut(s) 183, 688
AjnI CCWGG 3 cut(s) 159, 245, 601
AluBI AGCT 5 cut(s) 157, 385, 418, 436, 511
AluI AGCT 5 cut(s) 157, 385, 418, 436, 511
Alw21I GWGCWC 1 cut(s) 171
Alw26I GTCTC 3 cut(s) 229, 281, 389
Ama87I CYCGRG 2 cut(s) 335, 593
AoxI GGCC 4 cut(s) 300, 390, 750, 786
ApeKI GCWGC 3 cut(s) 415, 436, 798
AspLEI GCGC 7 cut(s) 62, 253, 470, 472, 517, 577, 601
AspS9I GGNCC 5 cut(s) 95, 300, 343, 355, 390
AsuHPI GGTGA 3 cut(s) 76, 131, 628
AvaI CYCGRG 2 cut(s) 335, 593
AvaII GGWCC 3 cut(s) 95, 343, 355
BanI GGYRCC 3 cut(s) 59, 250, 598
BbrPI CACGTG 2 cut(s) 317, 673
BbsI GAAGAC 1 cut(s) 262
Bbv12I GWGCWC 1 cut(s) 171
BbvI GCAGC 3 cut(s) 423, 427, 810
BccI CCATC 1 cut(s) 604
BceAI ACGGC 5 cut(s) 23, 162, 457, 737, 811
BcgI CGANNNNNNTGC 2 cut(s) 247, 281
BciT130I CCWGG 3 cut(s) 161, 247, 603
BclI TGATCA 1 cut(s) 561
BcoDI GTCTC 3 cut(s) 229, 281, 389
BfaI CTAG 1 cut(s) 642
BfoI RGCGCY 4 cut(s) 63, 254, 518, 602
BfrI CTTAAG 1 cut(s) 368
BfuAI ACCTGC 1 cut(s) 534
BisI GCNGC 7 cut(s) 36, 63, 416, 437, 462, 630, 799
BlpI GCTNAGC 1 cut(s) 518
BlsI GCNGC 7 cut(s) 37, 64, 417, 438, 463, 631, 800
Bme1390I CCNGG 3 cut(s) 161, 247, 603
Bme18I GGWCC 3 cut(s) 95, 343, 355
BmeT110I CYCGRG 2 cut(s) 335, 593
BmgBI CACGTC 2 cut(s) 183, 688
BmgT120I GGNCC 5 cut(s) 95, 300, 343, 355, 390
BmiI GGNNCC 6 cut(s) 61, 97, 252, 345, 356, 600
BmrFI CCNGG 3 cut(s) 161, 247, 603
BpiI GAAGAC 1 cut(s) 262
BplI GAGNNNNNCTC 4 cut(s) 425, 457, 767, 799
BpmI CTGGAG 2 cut(s) 61, 143
Bpu10I CCTNAGC 1 cut(s) 386
Bpu1102I GCTNAGC 1 cut(s) 518
BsaAI YACGTR 3 cut(s) 317, 491, 673
BsaBI GATNNNNATC 1 cut(s) 566
BsaHI GRCGYC 4 cut(s) 60, 234, 251, 599
BsaI GGTCTC 2 cut(s) 281, 389
BsaJI CCNNGG 8 cut(s) 55, 144, 246, 477, 578, 594, 717, 783
BsaWI WCCGGW 1 cut(s) 86
BsaXI ACNNNNNCTCC 2 cut(s) 524, 554
Bsc4I CCNNNNNNNGG 5 cut(s) 340, 352, 532, 748, 759
Bse8I GATNNNNATC 1 cut(s) 566
BseBI CCWGG 3 cut(s) 161, 247, 603
BseDI CCNNGG 8 cut(s) 55, 144, 246, 477, 578, 594, 717, 783
BseGI GGATG 1 cut(s) 615
BseJI GATNNNNATC 1 cut(s) 566
BseLI CCNNNNNNNGG 5 cut(s) 340, 352, 532, 748, 759
BseMII CTCAG 1 cut(s) 509
BsePI GCGCGC 1 cut(s) 468
BseRI GAGGAG 5 cut(s) 353, 433, 548, 661, 734
BseXI GCAGC 3 cut(s) 423, 427, 810
Bsh1236I CGCG 3 cut(s) 208, 470, 575
BshFI GGCC 4 cut(s) 302, 392, 752, 788
BshNI GGYRCC 3 cut(s) 59, 250, 598
BsiHKAI GWGCWC 1 cut(s) 171
BsiHKCI CYCGRG 2 cut(s) 335, 593
BsiSI CCGG 4 cut(s) 87, 380, 530, 733
BslFI GGGAC 1 cut(s) 569
BslI CCNNNNNNNGG 5 cut(s) 340, 352, 532, 748, 759
BsmAI GTCTC 3 cut(s) 229, 281, 389
BsmBI CGTCTC 1 cut(s) 229
BsmFI GGGAC 1 cut(s) 569
BsnI GGCC 4 cut(s) 302, 392, 752, 788
Bso31I GGTCTC 2 cut(s) 281, 389
BsoBI CYCGRG 2 cut(s) 335, 593
Bsp1286I GDGCHC 1 cut(s) 171
Bsp143I GATC 3 cut(s) 216, 561, 567
Bsp1720I GCTNAGC 1 cut(s) 518
Bsp68I TCGCGA 1 cut(s) 208
BspANI GGCC 4 cut(s) 302, 392, 752, 788
BspCNI CTCAG 1 cut(s) 510
BspFNI CGCG 3 cut(s) 208, 470, 575
BspHI TCATGA 1 cut(s) 564
BspLI GGNNCC 6 cut(s) 61, 97, 252, 345, 356, 600
BspMI ACCTGC 1 cut(s) 534
BspT107I GGYRCC 3 cut(s) 59, 250, 598
BspTI CTTAAG 1 cut(s) 368
BspTNI GGTCTC 2 cut(s) 281, 389
BssECI CCNNGG 8 cut(s) 55, 144, 246, 477, 578, 594, 717, 783
BssHII GCGCGC 1 cut(s) 468
BssMI GATC 3 cut(s) 216, 561, 567
BssNI GRCGYC 4 cut(s) 60, 234, 251, 599
BssT1I CCWWGG 1 cut(s) 578
Bst2UI CCWGG 3 cut(s) 161, 247, 603
Bst4CI ACNGT 3 cut(s) 364, 400, 810
BstACI GRCGYC 4 cut(s) 60, 234, 251, 599
BstAFI CTTAAG 1 cut(s) 368
BstBAI YACGTR 3 cut(s) 317, 491, 673
BstC8I GCNNGC 7 cut(s) 210, 413, 426, 466, 470, 509, 513
BstDEI CTNAG 3 cut(s) 189, 386, 518
BstDSI CCRYGG 1 cut(s) 144
BstENI CCTNNNNNAGG 3 cut(s) 338, 530, 746
BstF5I GGATG 1 cut(s) 615
BstFNI CGCG 3 cut(s) 208, 470, 575
BstH2I RGCGCY 4 cut(s) 63, 254, 518, 602
BstHHI GCGC 7 cut(s) 62, 253, 470, 472, 517, 577, 601
BstKTI GATC 3 cut(s) 219, 564, 570
BstMAI GTCTC 3 cut(s) 229, 281, 389
BstMBI GATC 3 cut(s) 216, 561, 567
BstMWI GCNNNNNNNGC 4 cut(s) 205, 421, 467, 638
BstNI CCWGG 3 cut(s) 161, 247, 603
BstSCI CCNGG 3 cut(s) 159, 245, 601
BstUI CGCG 3 cut(s) 208, 470, 575
BstV1I GCAGC 3 cut(s) 423, 427, 810
BstV2I GAAGAC 1 cut(s) 262
BsuRI GGCC 4 cut(s) 302, 392, 752, 788
BtgI CCRYGG 1 cut(s) 144
BtrI CACGTC 2 cut(s) 183, 688
BtsCI GGATG 1 cut(s) 615
BtuMI TCGCGA 1 cut(s) 208
BveI ACCTGC 1 cut(s) 534
Cac8I GCNNGC 7 cut(s) 210, 413, 426, 466, 470, 509, 513
CciI TCATGA 1 cut(s) 564
CfoI GCGC 7 cut(s) 62, 253, 470, 472, 517, 577, 601
Cfr13I GGNCC 5 cut(s) 95, 300, 343, 355, 390
CseI GACGC 5 cut(s) 211, 334, 581, 676, 812
Csp6I GTAC 3 cut(s) 448, 541, 739
CspCI CAANNNNNGTGG 2 cut(s) 298, 333
CviAII CATG 2 cut(s) 295, 565
CviQI GTAC 3 cut(s) 448, 541, 739
DdeI CTNAG 3 cut(s) 189, 386, 518
DinI GGCGCC 3 cut(s) 61, 252, 600
DpnI GATC 3 cut(s) 218, 563, 569
DpnII GATC 3 cut(s) 216, 561, 567
DraIII CACNNNGTG 1 cut(s) 688
EaeI YGGCCR 1 cut(s) 786
EciI GGCGGA 1 cut(s) 443
Eco130I CCWWGG 1 cut(s) 578
Eco31I GGTCTC 2 cut(s) 281, 389
Eco47I GGWCC 3 cut(s) 95, 343, 355
Eco57I CTGAAG 1 cut(s) 440
Eco72I CACGTG 2 cut(s) 317, 673
Eco88I CYCGRG 2 cut(s) 335, 593
EcoNI CCTNNNNNAGG 3 cut(s) 338, 530, 746
EcoO109I RGGNCCY 2 cut(s) 95, 343
EcoRII CCWGG 3 cut(s) 159, 245, 601
EcoT14I CCWWGG 1 cut(s) 578
EgeI GGCGCC 3 cut(s) 61, 252, 600
EheI GGCGCC 3 cut(s) 61, 252, 600
ErhI CCWWGG 1 cut(s) 578
Esp3I CGTCTC 1 cut(s) 229
FaeI CATG 2 cut(s) 298, 568
FaiI YATR 3 cut(s) 296, 566, 638
FaqI GGGAC 1 cut(s) 569
FatI CATG 2 cut(s) 294, 564
FauI CCCGC 2 cut(s) 204, 645
FbaI TGATCA 1 cut(s) 561
Fnu4HI GCNGC 7 cut(s) 36, 63, 416, 437, 462, 630, 799
FokI GGATG 1 cut(s) 622
Fsp4HI GCNGC 7 cut(s) 36, 63, 416, 437, 462, 630, 799
FspBI CTAG 1 cut(s) 642
GlaI GCGC 7 cut(s) 61, 252, 469, 471, 516, 576, 600
GluI GCNGC 7 cut(s) 36, 63, 416, 437, 462, 630, 799
GsuI CTGGAG 2 cut(s) 61, 143
HaeII RGCGCY 4 cut(s) 63, 254, 518, 602
HaeIII GGCC 4 cut(s) 302, 392, 752, 788
HapII CCGG 4 cut(s) 87, 380, 530, 733
HgaI GACGC 5 cut(s) 211, 334, 581, 676, 812
HhaI GCGC 7 cut(s) 62, 253, 470, 472, 517, 577, 601
Hin1I GRCGYC 4 cut(s) 60, 234, 251, 599
Hin1II CATG 2 cut(s) 298, 568
Hin6I GCGC 7 cut(s) 60, 251, 468, 470, 515, 575, 599
HinP1I GCGC 7 cut(s) 60, 251, 468, 470, 515, 575, 599
HincII GTYRAC 1 cut(s) 403
HindII GTYRAC 1 cut(s) 403
HinfI GANTC 4 cut(s) 7, 287, 374, 778
HpaI GTTAAC 1 cut(s) 403
HpaII CCGG 4 cut(s) 87, 380, 530, 733
HphI GGTGA 3 cut(s) 76, 131, 628
Hpy166II GTNNAC 3 cut(s) 403, 541, 691
Hpy188I TCNGA 3 cut(s) 457, 478, 609
Hpy188III TCNNGA 5 cut(s) 207, 358, 565, 593, 757
Hpy8I GTNNAC 3 cut(s) 403, 541, 691
Hpy99I CGWCG 7 cut(s) 236, 328, 575, 670, 689, 758, 806
HpyAV CCTTC 5 cut(s) 112, 238, 541, 691, 700
HpyCH4III ACNGT 3 cut(s) 364, 400, 810
HpyCH4IV ACGT 6 cut(s) 182, 234, 316, 490, 672, 687
HpyCH4V TGCA 3 cut(s) 258, 507, 682
HpyF10VI GCNNNNNNNGC 4 cut(s) 205, 421, 467, 638
HpyF3I CTNAG 3 cut(s) 189, 386, 518
HpySE526I ACGT 6 cut(s) 182, 234, 316, 490, 672, 687
Hsp92I GRCGYC 4 cut(s) 60, 234, 251, 599
Hsp92II CATG 2 cut(s) 298, 568
HspAI GCGC 7 cut(s) 60, 251, 468, 470, 515, 575, 599
KasI GGCGCC 3 cut(s) 59, 250, 598
Ksp22I TGATCA 1 cut(s) 561
KspAI GTTAAC 1 cut(s) 403
Kzo9I GATC 3 cut(s) 216, 561, 567
LmnI GCTCC 3 cut(s) 162, 174, 382
Lsp1109I GCAGC 3 cut(s) 423, 427, 810
MaeI CTAG 1 cut(s) 642
MaeII ACGT 6 cut(s) 182, 234, 316, 490, 672, 687
MaeIII GTNAC 1 cut(s) 584
MalI GATC 3 cut(s) 218, 563, 569
MboI GATC 3 cut(s) 216, 561, 567
MboII GAAGA 2 cut(s) 43, 262
MhlI GDGCHC 1 cut(s) 171
Mly113I GGCGCC 3 cut(s) 60, 251, 599
MlyI GAGTC 2 cut(s) 281, 368
MmeI TCCRAC 1 cut(s) 587
MseI TTAA 5 cut(s) 240, 369, 402, 747, 817
MspA1I CMGCKG 1 cut(s) 418
MspCI CTTAAG 1 cut(s) 368
MspI CCGG 4 cut(s) 87, 380, 530, 733
MspR9I CCNGG 3 cut(s) 161, 247, 603
MvaI CCWGG 3 cut(s) 161, 247, 603
MvnI CGCG 3 cut(s) 208, 470, 575
MwoI GCNNNNNNNGC 4 cut(s) 205, 421, 467, 638
NarI GGCGCC 3 cut(s) 60, 251, 599
NdeII GATC 3 cut(s) 216, 561, 567
NlaIII CATG 2 cut(s) 298, 568
NlaIV GGNNCC 6 cut(s) 61, 97, 252, 345, 356, 600
NmeAIII GCCGAG 7 cut(s) 36, 238, 258, 418, 605, 742, 764
NruI TCGCGA 1 cut(s) 208
PaeR7I CTCGAG 1 cut(s) 335
PagI TCATGA 1 cut(s) 564
PauI GCGCGC 1 cut(s) 468
PfeI GAWTC 1 cut(s) 778
PkrI GCNGC 7 cut(s) 37, 64, 417, 438, 463, 631, 800
PleI GAGTC 2 cut(s) 281, 368
PluTI GGCGCC 3 cut(s) 63, 254, 602
PmaCI CACGTG 2 cut(s) 317, 673
PmlI CACGTG 2 cut(s) 317, 673
PpsI GAGTC 2 cut(s) 281, 368
Ppu21I YACGTR 3 cut(s) 317, 491, 673
PpuMI RGGWCCY 2 cut(s) 95, 343
Psp5II RGGWCCY 2 cut(s) 95, 343
Psp6I CCWGG 3 cut(s) 159, 245, 601
PspCI CACGTG 2 cut(s) 317, 673
PspGI CCWGG 3 cut(s) 159, 245, 601
PspN4I GGNNCC 6 cut(s) 61, 97, 252, 345, 356, 600
PspPI GGNCC 5 cut(s) 95, 300, 343, 355, 390
PspPPI RGGWCCY 2 cut(s) 95, 343
PspXI VCTCGAGB 1 cut(s) 335
PteI GCGCGC 1 cut(s) 468
PvuII CAGCTG 1 cut(s) 418
RruI TCGCGA 1 cut(s) 208
RsaI GTAC 3 cut(s) 449, 542, 740
RsaNI GTAC 3 cut(s) 448, 541, 739
SaqAI TTAA 5 cut(s) 240, 369, 402, 747, 817
SatI GCNGC 7 cut(s) 36, 63, 416, 437, 462, 630, 799
Sau3AI GATC 3 cut(s) 216, 561, 567
Sau96I GGNCC 5 cut(s) 95, 300, 343, 355, 390
SchI GAGTC 2 cut(s) 281, 368
ScrFI CCNGG 3 cut(s) 161, 247, 603
SduI GDGCHC 1 cut(s) 171
SfoI GGCGCC 3 cut(s) 61, 252, 600
Sfr274I CTCGAG 1 cut(s) 335
SinI GGWCC 3 cut(s) 95, 343, 355
SlaI CTCGAG 1 cut(s) 335
SmlI CTYRAG 2 cut(s) 335, 368
SmoI CTYRAG 2 cut(s) 335, 368
SspDI GGCGCC 3 cut(s) 59, 250, 598
SspMI CTAG 1 cut(s) 642
StyD4I CCNGG 3 cut(s) 159, 245, 601
StyI CCWWGG 1 cut(s) 578
TaaI ACNGT 3 cut(s) 364, 400, 810
TaiI ACGT 6 cut(s) 185, 237, 319, 493, 675, 690
TaqI TCGA 3 cut(s) 231, 336, 570
TaqII GACCGA 1 cut(s) 263
TauI GCSGC 4 cut(s) 38, 65, 464, 632
TfiI GAWTC 1 cut(s) 778
Tru1I TTAA 5 cut(s) 240, 369, 402, 747, 817
Tru9I TTAA 5 cut(s) 240, 369, 402, 747, 817
TseI GCWGC 3 cut(s) 415, 436, 798
TspDTI ATGAA 1 cut(s) 61
Vha464I CTTAAG 1 cut(s) 368
VpaK11BI GGWCC 3 cut(s) 95, 343, 355
XagI CCTNNNNNAGG 3 cut(s) 338, 530, 746
XhoI CTCGAG 1 cut(s) 335
XspI CTAG 1 cut(s) 642
ZraI GACGTC 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.