FvH4_3g25190

Polysaccharide biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0005005
Physical Location & Seq
Reverse (-)
18166739 .. 18168455
1717 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g25190.t1

Sequence Viewer

Length: 843 bp
ATGAAGCATCGGAGCATCCGAACATCGGAGAAGACAGGTTTGCTAGCTGCAGGTTTGGTATTCTTCATCGGTGCTGCCCTATTCATCTCCAGCCTCACCGAAAATGGAAACTCATTCATTTGCTCTCTACCCATTTTCGGCAAGGATGATAAAATCCCCACAGCGATTCAATTACATGCCATTCTCCATTACGCCACATCACGAACAACACCGCAACTATCCAAGGATGAGATCAGCCTCGCCTTTGACGTCTTGAAATCACGAGCCCCATGCAACTTCCTCATATTTGGCCTCGGCCACGACTCGCTCATGTGGGTCTCATTCAACCCACGAGGCACGACACTGTTCCTCGAGGAGGACCCCAAGTGGCTCAAGAAAGCCTTGCAGGATGCTTCGCAGCTGCGTGCGGTACATGTGACCTACCACACGCAGCTGAAGCAGGCGGACGACCTGCTCACATCGTATAAATCCGAAACGATATGCAGGACGTCCGTGGCCGTGCTACATGGCAACACACATTGCAGGTTGGCATTGAGCAACCTACCGGACGAGGTGTACGAGAGGGAGTGGGACTTGATCATGATCGATGGGCCTCAGGGTTGGTTTGCCGAGGCGCCTGGTCGGATGAGTGTTATATTCGCGGTGGCTGTCATGGCCAGGAAAAGGCGAGGTCCGGGATCAACGCACGTGTTCTTGCATGACGTTGATCGGAAGGTGGAGAAGGCTTTTGCGGAGGAGTTTCTGTGCCGTAAATACCTTGTGAACGCCGTGGGGAGGCTCTGGCATTTTGTAATTCCGTCCGCCGTTGATGTCAACGATACTGACGACGCAAGTTTTTGCTAG

Protein Analysis

281

Amino Acids

31.37

Weight (kDa)

7.17

Isoelectric Point (pI)

28.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IRX15_IRX15L_GXM PF21729 77 - 263 4.7e-79 IRX15/IRX15L/GXM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 252, 491
AbsI CCTCGAGG 1 cut(s) 350
Acc36I ACCTGC 3 cut(s) 41, 459, 513
AccB1I GGYRCC 1 cut(s) 613
AccII CGCG 1 cut(s) 641
AciI CCGC 6 cut(s) 212, 407, 443, 641, 731, 801
AclWI GGATC 1 cut(s) 685
AcoI YGGCCR 3 cut(s) 295, 495, 654
AcuI CTGAAG 1 cut(s) 455
AcvI CACGTG 1 cut(s) 688
AcyI GRCGYC 3 cut(s) 249, 488, 614
AfaI GTAC 2 cut(s) 411, 557
AfiI CCNNNNNNNGG 5 cut(s) 25, 137, 355, 663, 774
AflIII ACRYGT 2 cut(s) 412, 687
AgsI TTSAA 3 cut(s) 170, 256, 325
AjnI CCWGG 2 cut(s) 616, 656
AluBI AGCT 3 cut(s) 47, 400, 433
AluI AGCT 3 cut(s) 47, 400, 433
Alw26I GTCTC 1 cut(s) 322
AlwI GGATC 1 cut(s) 685
Ama87I CYCGRG 1 cut(s) 350
AoxI GGCC 5 cut(s) 289, 295, 495, 590, 654
ApeKI GCWGC 5 cut(s) 47, 74, 397, 400, 430
AspLEI GCGC 1 cut(s) 616
AspS9I GGNCC 3 cut(s) 358, 590, 671
AsuC2I CCSGG 1 cut(s) 675
AsuHPI GGTGA 1 cut(s) 88
AsuNHI GCTAGC 1 cut(s) 43
AvaI CYCGRG 1 cut(s) 350
AvaII GGWCC 2 cut(s) 358, 671
AxyI CCTNAGG 1 cut(s) 594
BalI TGGCCA 1 cut(s) 656
BanI GGYRCC 1 cut(s) 613
BanII GRGCYC 1 cut(s) 268
BauI CACGAG 2 cut(s) 261, 330
BbrPI CACGTG 1 cut(s) 688
BbsI GAAGAC 1 cut(s) 38
BbvI GCAGC 5 cut(s) 34, 61, 387, 409, 442
BccI CCATC 1 cut(s) 581
BceAI ACGGC 4 cut(s) 482, 732, 752, 788
BcgI CGANNNNNNTGC 2 cut(s) 252, 286
BciT130I CCWGG 2 cut(s) 618, 658
BclI TGATCA 1 cut(s) 576
BcnI CCSGG 1 cut(s) 675
BcoDI GTCTC 1 cut(s) 322
BfaI CTAG 2 cut(s) 44, 841
BfmI CTRYAG 1 cut(s) 48
BfoI RGCGCY 1 cut(s) 617
BfuAI ACCTGC 3 cut(s) 41, 459, 513
BisI GCNGC 5 cut(s) 48, 75, 398, 401, 431
BlsI GCNGC 5 cut(s) 49, 76, 399, 402, 432
Bme1390I CCNGG 3 cut(s) 618, 658, 675
Bme18I GGWCC 2 cut(s) 358, 671
BmeT110I CYCGRG 1 cut(s) 350
BmgT120I GGNCC 3 cut(s) 358, 590, 671
BmiI GGNNCC 2 cut(s) 360, 615
BmrFI CCNGG 3 cut(s) 618, 658, 675
BmsI GCATC 3 cut(s) 16, 24, 379
BmtI GCTAGC 1 cut(s) 47
BpiI GAAGAC 1 cut(s) 38
BpmI CTGGAG 1 cut(s) 73
BpuEI CTTGAG 1 cut(s) 356
BpuMI CCSGG 1 cut(s) 675
Bsa29I ATCGAT 1 cut(s) 585
BsaAI YACGTR 1 cut(s) 688
BsaBI GATNNNNATC 1 cut(s) 581
BsaHI GRCGYC 3 cut(s) 249, 488, 614
BsaI GGTCTC 1 cut(s) 322
BsaJI CCNNGG 5 cut(s) 222, 292, 492, 609, 768
BsaWI WCCGGW 1 cut(s) 544
BsaXI ACNNNNNCTCC 2 cut(s) 728, 758
Bsc4I CCNNNNNNNGG 5 cut(s) 25, 137, 355, 663, 774
Bse21I CCTNAGG 1 cut(s) 594
Bse3DI GCAATG 1 cut(s) 517
Bse8I GATNNNNATC 1 cut(s) 581
BseBI CCWGG 2 cut(s) 618, 658
BseCI ATCGAT 1 cut(s) 585
BseDI CCNNGG 5 cut(s) 222, 292, 492, 609, 768
BseGI GGATG 5 cut(s) 15, 151, 232, 394, 630
BseJI GATNNNNATC 1 cut(s) 581
BseLI CCNNNNNNNGG 5 cut(s) 25, 137, 355, 663, 774
BseMI GCAATG 1 cut(s) 517
BseMII CTCAG 1 cut(s) 608
BseRI GAGGAG 2 cut(s) 368, 749
BseXI GCAGC 5 cut(s) 34, 61, 387, 409, 442
Bsh1236I CGCG 1 cut(s) 641
BshFI GGCC 5 cut(s) 291, 297, 497, 592, 656
BshNI GGYRCC 1 cut(s) 613
BshVI ATCGAT 1 cut(s) 585
BsiHKCI CYCGRG 1 cut(s) 350
BsiSI CCGG 2 cut(s) 545, 674
BslFI GGGAC 1 cut(s) 584
BslI CCNNNNNNNGG 5 cut(s) 25, 137, 355, 663, 774
BsmAI GTCTC 1 cut(s) 322
BsmFI GGGAC 1 cut(s) 584
BsnI GGCC 5 cut(s) 291, 297, 497, 592, 656
Bso31I GGTCTC 1 cut(s) 322
BsoBI CYCGRG 1 cut(s) 350
Bsp1286I GDGCHC 1 cut(s) 268
Bsp143I GATC 5 cut(s) 231, 576, 582, 677, 706
BspACI CCGC 6 cut(s) 212, 407, 443, 641, 731, 801
BspANI GGCC 5 cut(s) 291, 297, 497, 592, 656
BspCNI CTCAG 1 cut(s) 607
BspDI ATCGAT 1 cut(s) 585
BspFNI CGCG 1 cut(s) 641
BspHI TCATGA 1 cut(s) 579
BspLI GGNNCC 2 cut(s) 360, 615
BspMAI CTGCAG 1 cut(s) 52
BspMI ACCTGC 3 cut(s) 41, 459, 513
BspOI GCTAGC 1 cut(s) 47
BspPI GGATC 1 cut(s) 685
BspT107I GGYRCC 1 cut(s) 613
BspTNI GGTCTC 1 cut(s) 322
BsrDI GCAATG 1 cut(s) 517
BssECI CCNNGG 5 cut(s) 222, 292, 492, 609, 768
BssMI GATC 5 cut(s) 231, 576, 582, 677, 706
BssNI GRCGYC 3 cut(s) 249, 488, 614
BssSI CACGAG 2 cut(s) 261, 330
BssT1I CCWWGG 1 cut(s) 222
Bst2BI CACGAG 2 cut(s) 261, 330
Bst2UI CCWGG 2 cut(s) 618, 658
Bst4CI ACNGT 1 cut(s) 345
BstACI GRCGYC 3 cut(s) 249, 488, 614
BstBAI YACGTR 1 cut(s) 688
BstC8I GCNNGC 3 cut(s) 45, 405, 441
BstDEI CTNAG 1 cut(s) 594
BstDSI CCRYGG 2 cut(s) 492, 768
BstENI CCTNNNNNAGG 1 cut(s) 353
BstF5I GGATG 5 cut(s) 15, 151, 232, 394, 630
BstFNI CGCG 1 cut(s) 641
BstH2I RGCGCY 1 cut(s) 617
BstHHI GCGC 1 cut(s) 616
BstKTI GATC 5 cut(s) 234, 579, 585, 680, 709
BstMAI GTCTC 1 cut(s) 322
BstMBI GATC 5 cut(s) 231, 576, 582, 677, 706
BstMWI GCNNNNNNNGC 2 cut(s) 436, 653
BstNI CCWGG 2 cut(s) 618, 658
BstNSI RCATGY 2 cut(s) 179, 416
BstSCI CCNGG 3 cut(s) 616, 656, 673
BstSFI CTRYAG 1 cut(s) 48
BstUI CGCG 1 cut(s) 641
BstV1I GCAGC 5 cut(s) 34, 61, 387, 409, 442
BstV2I GAAGAC 1 cut(s) 38
Bsu15I ATCGAT 1 cut(s) 585
Bsu36I CCTNAGG 1 cut(s) 594
BsuRI GGCC 5 cut(s) 291, 297, 497, 592, 656
BsuTUI ATCGAT 1 cut(s) 585
BtgI CCRYGG 2 cut(s) 492, 768
BtsCI GGATG 5 cut(s) 15, 151, 232, 394, 630
BtsIMutI CAGTG 1 cut(s) 341
BveI ACCTGC 3 cut(s) 41, 459, 513
Cac8I GCNNGC 3 cut(s) 45, 405, 441
CciI TCATGA 1 cut(s) 579
CfoI GCGC 1 cut(s) 616
Cfr13I GGNCC 3 cut(s) 358, 590, 671
ClaI ATCGAT 1 cut(s) 585
CseI GACGC 1 cut(s) 836
Csp6I GTAC 2 cut(s) 410, 556
CviAII CATG 8 cut(s) 176, 270, 310, 413, 506, 580, 652, 698
CviQI GTAC 2 cut(s) 410, 556
DdeI CTNAG 1 cut(s) 594
DinI GGCGCC 1 cut(s) 615
DpnI GATC 5 cut(s) 233, 578, 584, 679, 708
DpnII GATC 5 cut(s) 231, 576, 582, 677, 706
EaeI YGGCCR 3 cut(s) 295, 495, 654
EciI GGCGGA 2 cut(s) 458, 790
Eco130I CCWWGG 1 cut(s) 222
Eco24I GRGCYC 1 cut(s) 268
Eco31I GGTCTC 1 cut(s) 322
Eco47I GGWCC 2 cut(s) 358, 671
Eco57I CTGAAG 1 cut(s) 455
Eco72I CACGTG 1 cut(s) 688
Eco81I CCTNAGG 1 cut(s) 594
Eco88I CYCGRG 1 cut(s) 350
EcoNI CCTNNNNNAGG 1 cut(s) 353
EcoO109I RGGNCCY 1 cut(s) 358
EcoRII CCWGG 2 cut(s) 616, 656
EcoT14I CCWWGG 1 cut(s) 222
EcoT38I GRGCYC 1 cut(s) 268
EgeI GGCGCC 1 cut(s) 615
EheI GGCGCC 1 cut(s) 615
ErhI CCWWGG 1 cut(s) 222
FaeI CATG 8 cut(s) 179, 273, 313, 416, 509, 583, 655, 701
FalI AAGNNNNNCTT 2 cut(s) 365, 397
FaqI GGGAC 1 cut(s) 584
FatI CATG 8 cut(s) 175, 269, 309, 412, 505, 579, 651, 697
FbaI TGATCA 1 cut(s) 576
Fnu4HI GCNGC 5 cut(s) 48, 75, 398, 401, 431
FokI GGATG 5 cut(s) 2, 158, 239, 401, 637
FriOI GRGCYC 1 cut(s) 268
Fsp4HI GCNGC 5 cut(s) 48, 75, 398, 401, 431
FspBI CTAG 2 cut(s) 44, 841
GlaI GCGC 1 cut(s) 615
GluI GCNGC 5 cut(s) 48, 75, 398, 401, 431
GsuI CTGGAG 1 cut(s) 73
HaeII RGCGCY 1 cut(s) 617
HaeIII GGCC 5 cut(s) 291, 297, 497, 592, 656
HapII CCGG 2 cut(s) 545, 674
HgaI GACGC 1 cut(s) 836
HhaI GCGC 1 cut(s) 616
Hin1I GRCGYC 3 cut(s) 249, 488, 614
Hin1II CATG 8 cut(s) 179, 273, 313, 416, 509, 583, 655, 701
Hin6I GCGC 1 cut(s) 614
HinP1I GCGC 1 cut(s) 614
HincII GTYRAC 1 cut(s) 814
HindII GTYRAC 1 cut(s) 814
HinfI GANTC 2 cut(s) 166, 302
HpaII CCGG 2 cut(s) 545, 674
HphI GGTGA 1 cut(s) 88
Hpy166II GTNNAC 3 cut(s) 556, 763, 814
Hpy188I TCNGA 6 cut(s) 12, 20, 28, 472, 624, 711
Hpy188III TCNNGA 5 cut(s) 201, 253, 261, 373, 580
Hpy8I GTNNAC 3 cut(s) 556, 763, 814
Hpy99I CGWCG 1 cut(s) 830
HpyAV CCTTC 2 cut(s) 706, 715
HpyCH4III ACNGT 1 cut(s) 345
HpyCH4IV ACGT 4 cut(s) 249, 488, 687, 702
HpyCH4V TGCA 6 cut(s) 50, 273, 385, 483, 522, 697
HpyF10VI GCNNNNNNNGC 2 cut(s) 436, 653
HpyF3I CTNAG 1 cut(s) 594
HpySE526I ACGT 4 cut(s) 249, 488, 687, 702
Hsp92I GRCGYC 3 cut(s) 249, 488, 614
Hsp92II CATG 8 cut(s) 179, 273, 313, 416, 509, 583, 655, 701
HspAI GCGC 1 cut(s) 614
KasI GGCGCC 1 cut(s) 613
Ksp22I TGATCA 1 cut(s) 576
Kzo9I GATC 5 cut(s) 231, 576, 582, 677, 706
LmnI GCTCC 1 cut(s) 12
Lsp1109I GCAGC 5 cut(s) 34, 61, 387, 409, 442
LweI GCATC 3 cut(s) 16, 24, 379
MaeI CTAG 2 cut(s) 44, 841
MaeII ACGT 4 cut(s) 249, 488, 687, 702
MaeIII GTNAC 1 cut(s) 415
MalI GATC 5 cut(s) 233, 578, 584, 679, 708
MboI GATC 5 cut(s) 231, 576, 582, 677, 706
MboII GAAGA 2 cut(s) 43, 55
MhlI GDGCHC 1 cut(s) 268
MlsI TGGCCA 1 cut(s) 656
MluCI AATT 2 cut(s) 170, 792
MluNI TGGCCA 1 cut(s) 656
Mly113I GGCGCC 1 cut(s) 614
MlyI GAGTC 1 cut(s) 296
MmeI TCCRAC 1 cut(s) 602
Mox20I TGGCCA 1 cut(s) 656
MscI TGGCCA 1 cut(s) 656
Msp20I TGGCCA 1 cut(s) 656
MspA1I CMGCKG 2 cut(s) 400, 433
MspI CCGG 2 cut(s) 545, 674
MspR9I CCNGG 3 cut(s) 618, 658, 675
MvaI CCWGG 2 cut(s) 618, 658
MvnI CGCG 1 cut(s) 641
MwoI GCNNNNNNNGC 2 cut(s) 436, 653
NarI GGCGCC 1 cut(s) 614
NciI CCSGG 1 cut(s) 675
NdeII GATC 5 cut(s) 231, 576, 582, 677, 706
NheI GCTAGC 1 cut(s) 43
NlaIII CATG 8 cut(s) 179, 273, 313, 416, 509, 583, 655, 701
NlaIV GGNNCC 2 cut(s) 360, 615
NmeAIII GCCGAG 2 cut(s) 273, 634
NmuCI GTSAC 1 cut(s) 415
NspI RCATGY 2 cut(s) 179, 416
PaeR7I CTCGAG 1 cut(s) 350
PagI TCATGA 1 cut(s) 579
PciI ACATGT 1 cut(s) 412
PcsI WCGNNNNNNNCGW 4 cut(s) 16, 246, 555, 822
PfeI GAWTC 1 cut(s) 166
PfoI TCCNGGA 1 cut(s) 673
PkrI GCNGC 5 cut(s) 49, 76, 399, 402, 432
PleI GAGTC 1 cut(s) 296
PluTI GGCGCC 1 cut(s) 617
PmaCI CACGTG 1 cut(s) 688
PmlI CACGTG 1 cut(s) 688
PpsI GAGTC 1 cut(s) 296
Ppu21I YACGTR 1 cut(s) 688
PpuMI RGGWCCY 1 cut(s) 358
PscI ACATGT 1 cut(s) 412
Psp5II RGGWCCY 1 cut(s) 358
Psp6I CCWGG 2 cut(s) 616, 656
PspCI CACGTG 1 cut(s) 688
PspGI CCWGG 2 cut(s) 616, 656
PspN4I GGNNCC 2 cut(s) 360, 615
PspPI GGNCC 3 cut(s) 358, 590, 671
PspPPI RGGWCCY 1 cut(s) 358
PspXI VCTCGAGB 1 cut(s) 350
PstI CTGCAG 1 cut(s) 52
PvuII CAGCTG 2 cut(s) 400, 433
RsaI GTAC 2 cut(s) 411, 557
RsaNI GTAC 2 cut(s) 410, 556
SatI GCNGC 5 cut(s) 48, 75, 398, 401, 431
Sau3AI GATC 5 cut(s) 231, 576, 582, 677, 706
Sau96I GGNCC 3 cut(s) 358, 590, 671
SchI GAGTC 1 cut(s) 296
ScrFI CCNGG 3 cut(s) 618, 658, 675
SduI GDGCHC 1 cut(s) 268
SfaNI GCATC 3 cut(s) 16, 24, 379
SfcI CTRYAG 1 cut(s) 48
SfoI GGCGCC 1 cut(s) 615
Sfr274I CTCGAG 1 cut(s) 350
SinI GGWCC 2 cut(s) 358, 671
SlaI CTCGAG 1 cut(s) 350
SmlI CTYRAG 2 cut(s) 350, 371
SmoI CTYRAG 2 cut(s) 350, 371
Sse9I AATT 2 cut(s) 170, 792
SsiI CCGC 6 cut(s) 212, 407, 443, 641, 731, 801
SspDI GGCGCC 1 cut(s) 613
SspMI CTAG 2 cut(s) 44, 841
StyD4I CCNGG 3 cut(s) 616, 656, 673
StyI CCWWGG 1 cut(s) 222
TaaI ACNGT 1 cut(s) 345
TaiI ACGT 4 cut(s) 252, 491, 690, 705
TaqI TCGA 2 cut(s) 351, 585
TasI AATT 2 cut(s) 170, 792
TfiI GAWTC 1 cut(s) 166
TscAI CASTG 1 cut(s) 348
TseFI GTSAC 1 cut(s) 415
TseI GCWGC 5 cut(s) 47, 74, 397, 400, 430
Tsp45I GTSAC 1 cut(s) 415
TspDTI ATGAA 4 cut(s) 17, 55, 73, 106
TspGWI ACGGA 2 cut(s) 481, 786
TspRI CASTG 1 cut(s) 348
VpaK11BI GGWCC 2 cut(s) 358, 671
XagI CCTNNNNNAGG 1 cut(s) 353
XceI RCATGY 2 cut(s) 179, 416
XhoI CTCGAG 1 cut(s) 350
XspI CTAG 2 cut(s) 44, 841
ZraI GACGTC 2 cut(s) 250, 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.