MD16G1120000.v1.1

Glucuronoxylan 4-O-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0005005
Physical Location & Seq
Reverse (-)
8600022 .. 8600876
855 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1120000.v1.1.491

Sequence Viewer

Length: 855 bp
ATGAAGAATCGGAGTCCATTTTCCGAGAGACCTGGGTTTCTCGCCGTGACTCTGGTTCTATTGATCGGCGCGGCGCTGGCCATCTCCAGTTTCTGCCAGGGTGGCACCTCCGTCCTCTACTCCATCGCCGGCCCAACATTTCTACGCGCCGTTGACGATAACTACTCGCCTGTGGCGATCCAGATCCAGGCCATCCTCCACTACGCCACGTCGCGCCAGACCCCGCAGCAGTCGCGAAAAGAGATCGGCGTGTCCTTCCAGGTCCTCAAGTCCCGCGCCCCCTGCAACTTCCTGGTGTTCGGCCTCGGCTTCGACTCCCTCATGTGGGCCTCCCTGAATCCACGCGGCACGACGCTGTTCCTGGAGGAGGATCCCAAGTGGGTCCAAAGCGTCCTCAAGGACGCCCCCCAGCTGCGGGCCCAGACGGTGCGCTACCGCACGCAGCTCAAGGAGGCGGACAAACTACTTTCCTCGTACCGCTCCAAACGGTCGTGCTCCCCGTCCGAGGCGGTCCTGCGCGGAAATACCGAGTGCAGGCTGGCACTGCACAACCTGCCGGACGAGGTGTACGACAAAGAGTGGGACCTGATCATGATCGACGCGCCGCGCGGGTATTTCCCGGAGGCGCCGGGGCGGATGGCGGCTATTTTCTCTGCAGCTGTCATGGCGAGGAGGAGGCGGGGATCCGGTGTGACACACGTATTCCTGCACGATGTGGATCGGAAGGTGGAGAAGGCGTTCGCGAATGAGTTCCTGTGCAGGAAGTTTTTGGTTAAGGCGGTGGGGAGGCTGTGGCACTTCGAGATCCCCTCCGCCGCAAACGTGACGCACGGTGACGGCTCGCGTTTTTGCTAA

Protein Analysis

285

Amino Acids

31.74

Weight (kDa)

9.55

Isoelectric Point (pI)

49.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IRX15_IRX15L_GXM PF21729 80 - 267 2.1e-83 IRX15/IRX15L/GXM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 561
AccB1I GGYRCC 2 cut(s) 104, 625
AccBSI CCGCTC 1 cut(s) 480
AclWI GGATC 8 cut(s) 172, 178, 365, 378, 678, 691, 726, 799
AcoI YGGCCR 1 cut(s) 78
AcyI GRCGYC 2 cut(s) 402, 626
AdeI CACNNNGTG 1 cut(s) 715
AfaI GTAC 2 cut(s) 476, 569
AfiI CCNNNNNNNGG 8 cut(s) 187, 324, 325, 367, 414, 415, 505, 534
AflIII ACRYGT 1 cut(s) 697
AjiI CACGTC 1 cut(s) 210
AjnI CCWGG 6 cut(s) 31, 96, 186, 258, 291, 360
AloI GAACNNNNNNTCC 2 cut(s) 722, 754
AluBI AGCT 3 cut(s) 412, 445, 659
AluI AGCT 3 cut(s) 412, 445, 659
Alw21I GWGCWC 1 cut(s) 497
Alw26I GTCTC 1 cut(s) 22
AlwI GGATC 8 cut(s) 172, 178, 365, 378, 678, 691, 726, 799
AlwNI CAGNNNCTG 1 cut(s) 93
AoxI GGCC 6 cut(s) 78, 130, 189, 301, 327, 417
ApaI GGGCCC 1 cut(s) 421
ApeKI GCWGC 4 cut(s) 226, 412, 442, 656
Asp700I GAANNNNTTC 2 cut(s) 737, 749
AspS9I GGNCC 8 cut(s) 131, 262, 327, 382, 417, 418, 511, 583
AsuC2I CCSGG 2 cut(s) 620, 630
AsuHPI GGTGA 1 cut(s) 845
AvaII GGWCC 4 cut(s) 262, 382, 511, 583
BaeGI GKGCMC 1 cut(s) 421
BalI TGGCCA 1 cut(s) 80
BamHI GGATCC 2 cut(s) 370, 683
BanI GGYRCC 2 cut(s) 104, 625
BanII GRGCYC 1 cut(s) 421
Bbv12I GWGCWC 1 cut(s) 497
BbvI GCAGC 4 cut(s) 238, 399, 454, 668
BccI CCATC 4 cut(s) 89, 131, 200, 631
BceAI ACGGC 3 cut(s) 29, 134, 853
BciT130I CCWGG 6 cut(s) 33, 98, 188, 260, 293, 362
BclI TGATCA 1 cut(s) 588
BcnI CCSGG 2 cut(s) 620, 630
BcoDI GTCTC 1 cut(s) 22
BfmI CTRYAG 1 cut(s) 654
BfoI RGCGCY 2 cut(s) 77, 629
BfuAI ACCTGC 1 cut(s) 561
BglI GCCNNNNNGGC 1 cut(s) 102
BisI GCNGC 9 cut(s) 72, 227, 346, 413, 443, 605, 642, 657, 816
BlsI GCNGC 9 cut(s) 73, 228, 347, 414, 444, 606, 643, 658, 817
Bme1390I CCNGG 8 cut(s) 33, 98, 188, 260, 293, 362, 620, 630
Bme18I GGWCC 4 cut(s) 262, 382, 511, 583
BmgBI CACGTC 1 cut(s) 210
BmgT120I GGNCC 8 cut(s) 131, 262, 327, 382, 417, 418, 511, 583
BmiI GGNNCC 7 cut(s) 106, 372, 383, 419, 584, 627, 685
BmrFI CCNGG 8 cut(s) 33, 98, 188, 260, 293, 362, 620, 630
BplI GAGNNNNNCTC 2 cut(s) 794, 826
BpmI CTGGAG 2 cut(s) 70, 383
BpuEI CTTGAG 3 cut(s) 251, 380, 431
BpuMI CCSGG 2 cut(s) 620, 630
BsaAI YACGTR 1 cut(s) 700
BsaBI GATNNNNATC 3 cut(s) 182, 593, 717
BsaHI GRCGYC 2 cut(s) 402, 626
BsaI GGTCTC 1 cut(s) 22
BsaJI CCNNGG 5 cut(s) 32, 97, 304, 504, 629
BsaWI WCCGGW 1 cut(s) 686
BsaXI ACNNNNNCTCC 2 cut(s) 722, 752
Bsc4I CCNNNNNNNGG 8 cut(s) 187, 324, 325, 367, 414, 415, 505, 534
Bse118I RCCGGY 1 cut(s) 128
Bse1I ACTGG 1 cut(s) 87
Bse8I GATNNNNATC 3 cut(s) 182, 593, 717
BseBI CCWGG 6 cut(s) 33, 98, 188, 260, 293, 362
BseDI CCNNGG 5 cut(s) 32, 97, 304, 504, 629
BseGI GGATG 2 cut(s) 192, 642
BseJI GATNNNNATC 3 cut(s) 182, 593, 717
BseLI CCNNNNNNNGG 8 cut(s) 187, 324, 325, 367, 414, 415, 505, 534
BseNI ACTGG 1 cut(s) 87
BseRI GAGGAG 3 cut(s) 380, 685, 688
BseSI GKGCMC 1 cut(s) 421
BseXI GCAGC 4 cut(s) 238, 399, 454, 668
BseYI CCCAGC 1 cut(s) 408
BsgI GTGCAG 4 cut(s) 530, 553, 692, 778
Bsh1285I CGRYCG 1 cut(s) 491
BshFI GGCC 6 cut(s) 80, 132, 191, 303, 329, 419
BshNI GGYRCC 2 cut(s) 104, 625
BsiEI CGRYCG 1 cut(s) 491
BsiHKAI GWGCWC 1 cut(s) 497
BsiSI CCGG 5 cut(s) 129, 557, 620, 629, 687
BslFI GGGAC 2 cut(s) 256, 596
BslI CCNNNNNNNGG 8 cut(s) 187, 324, 325, 367, 414, 415, 505, 534
BsmAI GTCTC 1 cut(s) 22
BsmFI GGGAC 2 cut(s) 256, 596
BsnI GGCC 6 cut(s) 80, 132, 191, 303, 329, 419
Bso31I GGTCTC 1 cut(s) 22
Bsp120I GGGCCC 1 cut(s) 417
Bsp1286I GDGCHC 2 cut(s) 421, 497
Bsp68I TCGCGA 2 cut(s) 235, 743
BspANI GGCC 6 cut(s) 80, 132, 191, 303, 329, 419
BspHI TCATGA 1 cut(s) 591
BspLI GGNNCC 7 cut(s) 106, 372, 383, 419, 584, 627, 685
BspMAI CTGCAG 1 cut(s) 658
BspMI ACCTGC 1 cut(s) 561
BspPI GGATC 8 cut(s) 172, 178, 365, 378, 678, 691, 726, 799
BspT107I GGYRCC 2 cut(s) 104, 625
BspTNI GGTCTC 1 cut(s) 22
BsrBI CCGCTC 1 cut(s) 480
BsrFI RCCGGY 1 cut(s) 128
BsrI ACTGG 1 cut(s) 87
BssAI RCCGGY 1 cut(s) 128
BssECI CCNNGG 5 cut(s) 32, 97, 304, 504, 629
BssNI GRCGYC 2 cut(s) 402, 626
Bst2UI CCWGG 6 cut(s) 33, 98, 188, 260, 293, 362
Bst4CI ACNGT 3 cut(s) 427, 489, 833
BstACI GRCGYC 2 cut(s) 402, 626
BstAPI GCANNNNNTGC 1 cut(s) 553
BstBAI YACGTR 1 cut(s) 700
BstC8I GCNNGC 7 cut(s) 78, 130, 417, 440, 536, 540, 842
BstENI CCTNNNNNAGG 1 cut(s) 365
BstF5I GGATG 2 cut(s) 192, 642
BstH2I RGCGCY 2 cut(s) 77, 629
BstMAI GTCTC 1 cut(s) 22
BstMCI CGRYCG 1 cut(s) 491
BstMWI GCNNNNNNNGC 7 cut(s) 77, 102, 232, 282, 544, 553, 665
BstNI CCWGG 6 cut(s) 33, 98, 188, 260, 293, 362
BstSCI CCNGG 8 cut(s) 31, 96, 186, 258, 291, 360, 618, 628
BstSFI CTRYAG 1 cut(s) 654
BstSLI GKGCMC 1 cut(s) 421
BstV1I GCAGC 4 cut(s) 238, 399, 454, 668
BstX2I RGATCY 4 cut(s) 183, 370, 683, 804
BstYI RGATCY 4 cut(s) 183, 370, 683, 804
BsuRI GGCC 6 cut(s) 80, 132, 191, 303, 329, 419
BtgZI GCGATG 1 cut(s) 109
BtrI CACGTC 1 cut(s) 210
BtsCI GGATG 2 cut(s) 192, 642
BtsI GCAGTG 1 cut(s) 542
BtsIMutI CAGTG 1 cut(s) 542
BtuMI TCGCGA 2 cut(s) 235, 743
BveI ACCTGC 1 cut(s) 561
Cac8I GCNNGC 7 cut(s) 78, 130, 417, 440, 536, 540, 842
CaiI CAGNNNCTG 1 cut(s) 93
CciI TCATGA 1 cut(s) 591
Cfr10I RCCGGY 1 cut(s) 128
Cfr13I GGNCC 8 cut(s) 131, 262, 327, 382, 417, 418, 511, 583
CseI GACGC 5 cut(s) 361, 379, 410, 608, 835
Csp6I GTAC 2 cut(s) 475, 568
CviAII CATG 3 cut(s) 322, 592, 664
CviQI GTAC 2 cut(s) 475, 568
DinI GGCGCC 1 cut(s) 627
DraIII CACNNNGTG 1 cut(s) 715
EaeI YGGCCR 1 cut(s) 78
EciI GGCGGA 3 cut(s) 470, 649, 802
Eco24I GRGCYC 1 cut(s) 421
Eco31I GGTCTC 1 cut(s) 22
Eco47I GGWCC 4 cut(s) 262, 382, 511, 583
EcoNI CCTNNNNNAGG 1 cut(s) 365
EcoO109I RGGNCCY 2 cut(s) 262, 583
EcoRII CCWGG 6 cut(s) 31, 96, 186, 258, 291, 360
EcoT38I GRGCYC 1 cut(s) 421
EgeI GGCGCC 1 cut(s) 627
EheI GGCGCC 1 cut(s) 627
FaeI CATG 3 cut(s) 325, 595, 667
FaiI YATR 3 cut(s) 323, 593, 665
FaqI GGGAC 2 cut(s) 256, 596
FatI CATG 3 cut(s) 321, 591, 663
FauI CCCGC 5 cut(s) 231, 281, 408, 602, 672
FbaI TGATCA 1 cut(s) 588
Fnu4HI GCNGC 9 cut(s) 72, 227, 346, 413, 443, 605, 642, 657, 816
FokI GGATG 2 cut(s) 179, 649
FriOI GRGCYC 1 cut(s) 421
Fsp4HI GCNGC 9 cut(s) 72, 227, 346, 413, 443, 605, 642, 657, 816
GluI GCNGC 9 cut(s) 72, 227, 346, 413, 443, 605, 642, 657, 816
GsaI CCCAGC 1 cut(s) 412
GsuI CTGGAG 2 cut(s) 70, 383
HaeII RGCGCY 2 cut(s) 77, 629
HaeIII GGCC 6 cut(s) 80, 132, 191, 303, 329, 419
HapII CCGG 5 cut(s) 129, 557, 620, 629, 687
HgaI GACGC 5 cut(s) 361, 379, 410, 608, 835
Hin1I GRCGYC 2 cut(s) 402, 626
Hin1II CATG 3 cut(s) 325, 595, 667
HincII GTYRAC 1 cut(s) 154
HindII GTYRAC 1 cut(s) 154
HinfI GANTC 5 cut(s) 7, 13, 49, 314, 337
HpaII CCGG 5 cut(s) 129, 557, 620, 629, 687
HphI GGTGA 1 cut(s) 845
Hpy166II GTNNAC 2 cut(s) 154, 568
Hpy188I TCNGA 4 cut(s) 12, 25, 505, 723
Hpy188III TCNNGA 5 cut(s) 181, 234, 592, 742, 802
Hpy8I GTNNAC 2 cut(s) 154, 568
Hpy99I CGWCG 3 cut(s) 214, 355, 602
HpyAV CCTTC 3 cut(s) 265, 718, 727
HpyCH4III ACNGT 3 cut(s) 427, 489, 833
HpyCH4IV ACGT 3 cut(s) 209, 699, 822
HpyCH4V TGCA 6 cut(s) 285, 534, 547, 656, 709, 759
HpyF10VI GCNNNNNNNGC 7 cut(s) 77, 102, 232, 282, 544, 553, 665
HpySE526I ACGT 3 cut(s) 209, 699, 822
Hsp92I GRCGYC 2 cut(s) 402, 626
Hsp92II CATG 3 cut(s) 325, 595, 667
KasI GGCGCC 1 cut(s) 625
KroI GCCGGC 1 cut(s) 128
KroNI GCCGGC 1 cut(s) 130
Ksp22I TGATCA 1 cut(s) 588
LmnI GCTCC 2 cut(s) 485, 500
Lsp1109I GCAGC 4 cut(s) 238, 399, 454, 668
MaeII ACGT 3 cut(s) 209, 699, 822
MaeIII GTNAC 4 cut(s) 46, 691, 823, 833
MbiI CCGCTC 1 cut(s) 480
MboII GAAGA 1 cut(s) 16
MflI RGATCY 4 cut(s) 183, 370, 683, 804
MhlI GDGCHC 2 cut(s) 421, 497
MlsI TGGCCA 1 cut(s) 80
MluNI TGGCCA 1 cut(s) 80
Mly113I GGCGCC 1 cut(s) 626
MlyI GAGTC 3 cut(s) 22, 43, 308
Mox20I TGGCCA 1 cut(s) 80
MroNI GCCGGC 1 cut(s) 128
MroXI GAANNNNTTC 2 cut(s) 737, 749
MscI TGGCCA 1 cut(s) 80
MseI TTAA 1 cut(s) 774
Msp20I TGGCCA 1 cut(s) 80
MspA1I CMGCKG 2 cut(s) 412, 659
MspI CCGG 5 cut(s) 129, 557, 620, 629, 687
MspR9I CCNGG 8 cut(s) 33, 98, 188, 260, 293, 362, 620, 630
MteI GCGCNGCGC 2 cut(s) 72, 605
MvaI CCWGG 6 cut(s) 33, 98, 188, 260, 293, 362
MwoI GCNNNNNNNGC 7 cut(s) 77, 102, 232, 282, 544, 553, 665
NaeI GCCGGC 1 cut(s) 130
NarI GGCGCC 1 cut(s) 626
NciI CCSGG 2 cut(s) 620, 630
NgoMIV GCCGGC 1 cut(s) 128
NlaIII CATG 3 cut(s) 325, 595, 667
NlaIV GGNNCC 7 cut(s) 106, 372, 383, 419, 584, 627, 685
NmeAIII GCCGAG 1 cut(s) 285
NmuCI GTSAC 4 cut(s) 46, 691, 823, 833
NruI TCGCGA 2 cut(s) 235, 743
PagI TCATGA 1 cut(s) 591
PcsI WCGNNNNNNNCGW 3 cut(s) 173, 497, 567
PdiI GCCGGC 1 cut(s) 130
PdmI GAANNNNTTC 2 cut(s) 737, 749
PfeI GAWTC 2 cut(s) 7, 337
PfoI TCCNGGA 2 cut(s) 360, 618
PkrI GCNGC 9 cut(s) 73, 228, 347, 414, 444, 606, 643, 658, 817
PleI GAGTC 3 cut(s) 21, 43, 308
PluTI GGCGCC 1 cut(s) 629
PpsI GAGTC 3 cut(s) 21, 43, 308
Ppu21I YACGTR 1 cut(s) 700
PpuMI RGGWCCY 2 cut(s) 262, 583
Psp5II RGGWCCY 2 cut(s) 262, 583
Psp6I CCWGG 6 cut(s) 31, 96, 186, 258, 291, 360
PspFI CCCAGC 1 cut(s) 408
PspGI CCWGG 6 cut(s) 31, 96, 186, 258, 291, 360
PspN4I GGNNCC 7 cut(s) 106, 372, 383, 419, 584, 627, 685
PspOMI GGGCCC 1 cut(s) 417
PspPI GGNCC 8 cut(s) 131, 262, 327, 382, 417, 418, 511, 583
PspPPI RGGWCCY 2 cut(s) 262, 583
PstI CTGCAG 1 cut(s) 658
PstNI CAGNNNCTG 1 cut(s) 93
PsuI RGATCY 4 cut(s) 183, 370, 683, 804
PvuII CAGCTG 2 cut(s) 412, 659
RruI TCGCGA 2 cut(s) 235, 743
RsaI GTAC 2 cut(s) 476, 569
RsaNI GTAC 2 cut(s) 475, 568
SaqAI TTAA 1 cut(s) 774
SatI GCNGC 9 cut(s) 72, 227, 346, 413, 443, 605, 642, 657, 816
Sau96I GGNCC 8 cut(s) 131, 262, 327, 382, 417, 418, 511, 583
SchI GAGTC 3 cut(s) 22, 43, 308
ScrFI CCNGG 8 cut(s) 33, 98, 188, 260, 293, 362, 620, 630
SduI GDGCHC 2 cut(s) 421, 497
SfcI CTRYAG 1 cut(s) 654
SfoI GGCGCC 1 cut(s) 627
SinI GGWCC 4 cut(s) 262, 382, 511, 583
SmlI CTYRAG 3 cut(s) 266, 395, 446
SmoI CTYRAG 3 cut(s) 266, 395, 446
SspDI GGCGCC 1 cut(s) 625
StyD4I CCNGG 8 cut(s) 31, 96, 186, 258, 291, 360, 618, 628
TaaI ACNGT 3 cut(s) 427, 489, 833
TaiI ACGT 3 cut(s) 212, 702, 825
TaqI TCGA 3 cut(s) 312, 597, 801
TauI GCSGC 5 cut(s) 74, 348, 607, 644, 818
TfiI GAWTC 2 cut(s) 7, 337
Tru1I TTAA 1 cut(s) 774
Tru9I TTAA 1 cut(s) 774
TscAI CASTG 1 cut(s) 549
TseFI GTSAC 4 cut(s) 46, 691, 823, 833
TseI GCWGC 4 cut(s) 226, 412, 442, 656
Tsp45I GTSAC 4 cut(s) 46, 691, 823, 833
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 100
TspRI CASTG 1 cut(s) 549
VpaK11BI GGWCC 4 cut(s) 262, 382, 511, 583
XagI CCTNNNNNAGG 1 cut(s) 365
XmnI GAANNNNTTC 2 cut(s) 737, 749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.