Rh5BG314600

Polysaccharide biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0005005
Physical Location & Seq
Reverse (-)
41723316 .. 41724773
1458 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG314600.1

Sequence Viewer

Length: 843 bp
ATGAAGCATCGGAGCACTCTAACATCGGAGAAGACGGGTCTCCTAGCTGCAGTTTTGGTTTTCTTTATTGGTGCTGCTTTGTTCATCTCCAGCCTCACCGAAACTGGAAACTCATTCTTTTGCTCTTTACCCATTTTTGGCAAGGACGATAATGCCCCCACAGCGATTCAACTACAGGCAATTCTCCATTACGCAACATCTCGAACAACGCCGCAGCAGTCCAAGGATGAGATTAGCGTCTCCTTTGAGGTCTTGAAATCACGAGCGCCATGCAACTTCCTCGTATTTGGTCTCGGCCACGACTCGCTGATGTGGGCCTCATTTAACCCACATGGCCCGACGTTGTTCCTCGAGGAGGACCCCAAGTGGGTCAAGAATGTCTTAAACAAGGCGCCGCAGCTGCGTGCGGTACATGTGAACTACCGCACGCAGCTCAAGCAAGCGAACGACCTACTATTGTCGTACAAATCAGAAGCGACGTGTAGGCCGTCCGAGACAGTGCTACGTGGCAACACACAGTGCAAGTTAGCGCTGAGCAACCTACCAGATGAGGTGTACGAGAGGGAGTGGGACCTGATTATGATCGATGCGCCTCGGGGTTGGTTTGCCGAGGCGCCTGGTCGAATGGGTGCTATATTCTCAGCTGCTGTCATGGCCAGGAAGAGGCAGGGACCGGGCTCGACGCACGTGTTCTTGCATGACATTGATCGAAAGGTGGAGAAGGCTTTTGCGGAGGAGTTCTTGTGCCGTAAGTATCTTGTGAACGCCGTGGGGAGGCTGTGGCATTTTGTGATTCCGTCTGCCGTTGATGTCACCAACGCTGACAACAAGCATTTTTGTTAA

Protein Analysis

280

Amino Acids

31.35

Weight (kDa)

8.49

Isoelectric Point (pI)

31.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IRX15_IRX15L_GXM PF21729 77 - 263 1e-83 IRX15/IRX15L/GXM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 350
AccB1I GGYRCC 2 cut(s) 391, 613
AciI CCGC 5 cut(s) 212, 395, 407, 424, 731
AcoI YGGCCR 2 cut(s) 295, 654
AcvI CACGTG 1 cut(s) 688
AcyI GRCGYC 2 cut(s) 392, 614
AdeI CACNNNGTG 1 cut(s) 519
AfaI GTAC 3 cut(s) 411, 464, 557
AfeI AGCGCT 1 cut(s) 531
AfiI CCNNNNNNNGG 5 cut(s) 137, 355, 367, 663, 774
AflIII ACRYGT 3 cut(s) 412, 479, 687
AgsI TTSAA 2 cut(s) 170, 256
AjiI CACGTC 1 cut(s) 480
AjnI CCWGG 2 cut(s) 616, 656
AluBI AGCT 4 cut(s) 47, 400, 433, 644
AluI AGCT 4 cut(s) 47, 400, 433, 644
Alw21I GWGCWC 1 cut(s) 17
Alw26I GTCTC 4 cut(s) 44, 244, 296, 488
AlwNI CAGNNNCTG 1 cut(s) 647
Ama87I CYCGRG 2 cut(s) 350, 594
Aor51HI AGCGCT 1 cut(s) 531
AoxI GGCC 5 cut(s) 295, 315, 334, 485, 654
ApeKI GCWGC 7 cut(s) 47, 74, 214, 397, 400, 430, 644
AspLEI GCGC 5 cut(s) 268, 394, 532, 592, 616
AspS9I GGNCC 5 cut(s) 315, 335, 358, 571, 671
AsuC2I CCSGG 1 cut(s) 675
AsuHPI GGTGA 2 cut(s) 88, 805
AvaI CYCGRG 2 cut(s) 350, 594
AvaII GGWCC 3 cut(s) 358, 571, 671
BalI TGGCCA 1 cut(s) 656
BanI GGYRCC 2 cut(s) 391, 613
BanII GRGCYC 1 cut(s) 680
BauI CACGAG 1 cut(s) 261
BbrPI CACGTG 1 cut(s) 688
BbsI GAAGAC 1 cut(s) 38
Bbv12I GWGCWC 1 cut(s) 17
BbvI GCAGC 7 cut(s) 34, 61, 226, 387, 409, 442, 631
BceAI ACGGC 4 cut(s) 472, 732, 752, 788
BcgI CGANNNNNNTGC 4 cut(s) 252, 262, 286, 296
BciT130I CCWGG 2 cut(s) 618, 658
BcnI CCSGG 1 cut(s) 675
BcoDI GTCTC 4 cut(s) 44, 244, 296, 488
BfaI CTAG 1 cut(s) 44
BfmI CTRYAG 2 cut(s) 48, 173
BfoI RGCGCY 4 cut(s) 269, 395, 533, 617
BisI GCNGC 9 cut(s) 48, 75, 212, 215, 395, 398, 401, 431, 645
BlpI GCTNAGC 1 cut(s) 533
BlsI GCNGC 9 cut(s) 49, 76, 213, 216, 396, 399, 402, 432, 646
Bme1390I CCNGG 3 cut(s) 618, 658, 675
Bme18I GGWCC 3 cut(s) 358, 571, 671
BmeT110I CYCGRG 2 cut(s) 350, 594
BmgBI CACGTC 1 cut(s) 480
BmgT120I GGNCC 5 cut(s) 315, 335, 358, 571, 671
BmiI GGNNCC 5 cut(s) 360, 393, 572, 615, 672
BmrFI CCNGG 3 cut(s) 618, 658, 675
BmsI GCATC 2 cut(s) 16, 577
BpiI GAAGAC 1 cut(s) 38
BpmI CTGGAG 1 cut(s) 73
Bpu1102I GCTNAGC 1 cut(s) 533
BpuEI CTTGAG 1 cut(s) 419
BpuMI CCSGG 1 cut(s) 675
Bsa29I ATCGAT 1 cut(s) 585
BsaAI YACGTR 2 cut(s) 506, 688
BsaBI GATNNNNATC 1 cut(s) 581
BsaHI GRCGYC 2 cut(s) 392, 614
BsaI GGTCTC 2 cut(s) 44, 296
BsaJI CCNNGG 4 cut(s) 222, 593, 609, 768
BsaXI ACNNNNNCTCC 2 cut(s) 728, 758
Bsc4I CCNNNNNNNGG 5 cut(s) 137, 355, 367, 663, 774
Bse1I ACTGG 1 cut(s) 109
Bse8I GATNNNNATC 1 cut(s) 581
BseBI CCWGG 2 cut(s) 618, 658
BseCI ATCGAT 1 cut(s) 585
BseDI CCNNGG 4 cut(s) 222, 593, 609, 768
BseGI GGATG 1 cut(s) 232
BseJI GATNNNNATC 1 cut(s) 581
BseLI CCNNNNNNNGG 5 cut(s) 137, 355, 367, 663, 774
BseMII CTCAG 2 cut(s) 524, 654
BseNI ACTGG 1 cut(s) 109
BseRI GAGGAG 2 cut(s) 368, 749
BseXI GCAGC 7 cut(s) 34, 61, 226, 387, 409, 442, 631
BshFI GGCC 5 cut(s) 297, 317, 336, 487, 656
BshNI GGYRCC 2 cut(s) 391, 613
BshVI ATCGAT 1 cut(s) 585
BsiHKAI GWGCWC 1 cut(s) 17
BsiHKCI CYCGRG 2 cut(s) 350, 594
BsiSI CCGG 1 cut(s) 674
BslFI GGGAC 2 cut(s) 584, 684
BslI CCNNNNNNNGG 5 cut(s) 137, 355, 367, 663, 774
BsmAI GTCTC 4 cut(s) 44, 244, 296, 488
BsmBI CGTCTC 1 cut(s) 244
BsmFI GGGAC 2 cut(s) 584, 684
BsnI GGCC 5 cut(s) 297, 317, 336, 487, 656
Bso31I GGTCTC 2 cut(s) 44, 296
BsoBI CYCGRG 2 cut(s) 350, 594
Bsp1286I GDGCHC 2 cut(s) 17, 680
Bsp143I GATC 2 cut(s) 582, 706
Bsp1720I GCTNAGC 1 cut(s) 533
BspACI CCGC 5 cut(s) 212, 395, 407, 424, 731
BspANI GGCC 5 cut(s) 297, 317, 336, 487, 656
BspCNI CTCAG 2 cut(s) 525, 653
BspDI ATCGAT 1 cut(s) 585
BspLI GGNNCC 5 cut(s) 360, 393, 572, 615, 672
BspMAI CTGCAG 1 cut(s) 52
BspT107I GGYRCC 2 cut(s) 391, 613
BspTNI GGTCTC 2 cut(s) 44, 296
BsrI ACTGG 1 cut(s) 109
BssECI CCNNGG 4 cut(s) 222, 593, 609, 768
BssMI GATC 2 cut(s) 582, 706
BssNI GRCGYC 2 cut(s) 392, 614
BssSI CACGAG 1 cut(s) 261
BssT1I CCWWGG 1 cut(s) 222
Bst2BI CACGAG 1 cut(s) 261
Bst2UI CCWGG 2 cut(s) 618, 658
Bst4CI ACNGT 2 cut(s) 499, 519
Bst6I CTCTTC 1 cut(s) 656
BstACI GRCGYC 2 cut(s) 392, 614
BstBAI YACGTR 2 cut(s) 506, 688
BstC8I GCNNGC 3 cut(s) 405, 428, 441
BstDEI CTNAG 2 cut(s) 533, 640
BstDSI CCRYGG 1 cut(s) 768
BstENI CCTNNNNNAGG 1 cut(s) 353
BstF5I GGATG 1 cut(s) 232
BstH2I RGCGCY 4 cut(s) 269, 395, 533, 617
BstHHI GCGC 5 cut(s) 268, 394, 532, 592, 616
BstKTI GATC 2 cut(s) 585, 709
BstMAI GTCTC 4 cut(s) 44, 244, 296, 488
BstMBI GATC 2 cut(s) 582, 706
BstMWI GCNNNNNNNGC 4 cut(s) 161, 400, 436, 653
BstNI CCWGG 2 cut(s) 618, 658
BstNSI RCATGY 1 cut(s) 416
BstSCI CCNGG 3 cut(s) 616, 656, 673
BstSFI CTRYAG 2 cut(s) 48, 173
BstV1I GCAGC 7 cut(s) 34, 61, 226, 387, 409, 442, 631
BstV2I GAAGAC 1 cut(s) 38
Bsu15I ATCGAT 1 cut(s) 585
BsuRI GGCC 5 cut(s) 297, 317, 336, 487, 656
BsuTUI ATCGAT 1 cut(s) 585
BtgI CCRYGG 1 cut(s) 768
BtrI CACGTC 1 cut(s) 480
BtsCI GGATG 1 cut(s) 232
BtsIMutI CAGTG 2 cut(s) 504, 524
Cac8I GCNNGC 3 cut(s) 405, 428, 441
CaiI CAGNNNCTG 1 cut(s) 647
CfoI GCGC 5 cut(s) 268, 394, 532, 592, 616
Cfr13I GGNCC 5 cut(s) 315, 335, 358, 571, 671
ClaI ATCGAT 1 cut(s) 585
CseI GACGC 2 cut(s) 226, 691
Csp6I GTAC 3 cut(s) 410, 463, 556
CviAII CATG 5 cut(s) 270, 332, 413, 652, 698
CviQI GTAC 3 cut(s) 410, 463, 556
DdeI CTNAG 2 cut(s) 533, 640
DinI GGCGCC 2 cut(s) 393, 615
DpnI GATC 2 cut(s) 584, 708
DpnII GATC 2 cut(s) 582, 706
DraIII CACNNNGTG 1 cut(s) 519
EaeI YGGCCR 2 cut(s) 295, 654
Eam1104I CTCTTC 1 cut(s) 656
EarI CTCTTC 1 cut(s) 656
Eco130I CCWWGG 1 cut(s) 222
Eco24I GRGCYC 1 cut(s) 680
Eco31I GGTCTC 2 cut(s) 44, 296
Eco47I GGWCC 3 cut(s) 358, 571, 671
Eco47III AGCGCT 1 cut(s) 531
Eco72I CACGTG 1 cut(s) 688
Eco88I CYCGRG 2 cut(s) 350, 594
EcoNI CCTNNNNNAGG 1 cut(s) 353
EcoO109I RGGNCCY 2 cut(s) 358, 571
EcoRII CCWGG 2 cut(s) 616, 656
EcoT14I CCWWGG 1 cut(s) 222
EcoT38I GRGCYC 1 cut(s) 680
EgeI GGCGCC 2 cut(s) 393, 615
EheI GGCGCC 2 cut(s) 393, 615
ErhI CCWWGG 1 cut(s) 222
Esp3I CGTCTC 1 cut(s) 244
FaeI CATG 5 cut(s) 273, 335, 416, 655, 701
FaiI YATR 7 cut(s) 271, 333, 414, 581, 635, 653, 699
FalI AAGNNNNNCTT 2 cut(s) 365, 397
FaqI GGGAC 2 cut(s) 584, 684
FatI CATG 5 cut(s) 269, 331, 412, 651, 697
Fnu4HI GCNGC 9 cut(s) 48, 75, 212, 215, 395, 398, 401, 431, 645
FokI GGATG 1 cut(s) 239
FriOI GRGCYC 1 cut(s) 680
Fsp4HI GCNGC 9 cut(s) 48, 75, 212, 215, 395, 398, 401, 431, 645
FspBI CTAG 1 cut(s) 44
GlaI GCGC 5 cut(s) 267, 393, 531, 591, 615
GluI GCNGC 9 cut(s) 48, 75, 212, 215, 395, 398, 401, 431, 645
GsuI CTGGAG 1 cut(s) 73
HaeII RGCGCY 4 cut(s) 269, 395, 533, 617
HaeIII GGCC 5 cut(s) 297, 317, 336, 487, 656
HapII CCGG 1 cut(s) 674
HgaI GACGC 2 cut(s) 226, 691
HhaI GCGC 5 cut(s) 268, 394, 532, 592, 616
Hin1I GRCGYC 2 cut(s) 392, 614
Hin1II CATG 5 cut(s) 273, 335, 416, 655, 701
Hin6I GCGC 5 cut(s) 266, 392, 530, 590, 614
HinP1I GCGC 5 cut(s) 266, 392, 530, 590, 614
HinfI GANTC 3 cut(s) 166, 302, 793
HpaII CCGG 1 cut(s) 674
HphI GGTGA 2 cut(s) 88, 805
Hpy166II GTNNAC 3 cut(s) 418, 556, 763
Hpy188I TCNGA 4 cut(s) 12, 28, 472, 493
Hpy188III TCNNGA 4 cut(s) 201, 253, 261, 373
Hpy8I GTNNAC 3 cut(s) 418, 556, 763
Hpy99I CGWCG 3 cut(s) 343, 481, 685
HpyAV CCTTC 1 cut(s) 715
HpyCH4III ACNGT 2 cut(s) 499, 519
HpyCH4IV ACGT 4 cut(s) 341, 479, 505, 687
HpyCH4V TGCA 4 cut(s) 50, 273, 522, 697
HpyF10VI GCNNNNNNNGC 4 cut(s) 161, 400, 436, 653
HpyF3I CTNAG 2 cut(s) 533, 640
HpySE526I ACGT 4 cut(s) 341, 479, 505, 687
Hsp92I GRCGYC 2 cut(s) 392, 614
Hsp92II CATG 5 cut(s) 273, 335, 416, 655, 701
HspAI GCGC 5 cut(s) 266, 392, 530, 590, 614
KasI GGCGCC 2 cut(s) 391, 613
Kzo9I GATC 2 cut(s) 582, 706
LmnI GCTCC 1 cut(s) 12
Lsp1109I GCAGC 7 cut(s) 34, 61, 226, 387, 409, 442, 631
LweI GCATC 2 cut(s) 16, 577
MaeI CTAG 1 cut(s) 44
MaeII ACGT 4 cut(s) 341, 479, 505, 687
MaeIII GTNAC 1 cut(s) 811
MalI GATC 2 cut(s) 584, 708
MboI GATC 2 cut(s) 582, 706
MboII GAAGA 2 cut(s) 43, 673
MhlI GDGCHC 2 cut(s) 17, 680
MlsI TGGCCA 1 cut(s) 656
MluCI AATT 1 cut(s) 180
MluNI TGGCCA 1 cut(s) 656
Mly113I GGCGCC 2 cut(s) 392, 614
MlyI GAGTC 1 cut(s) 296
Mox20I TGGCCA 1 cut(s) 656
MscI TGGCCA 1 cut(s) 656
MseI TTAA 3 cut(s) 324, 383, 841
Msp20I TGGCCA 1 cut(s) 656
MspA1I CMGCKG 2 cut(s) 400, 644
MspI CCGG 1 cut(s) 674
MspR9I CCNGG 3 cut(s) 618, 658, 675
MvaI CCWGG 2 cut(s) 618, 658
MwoI GCNNNNNNNGC 4 cut(s) 161, 400, 436, 653
NarI GGCGCC 2 cut(s) 392, 614
NciI CCSGG 1 cut(s) 675
NdeII GATC 2 cut(s) 582, 706
NlaIII CATG 5 cut(s) 273, 335, 416, 655, 701
NlaIV GGNNCC 5 cut(s) 360, 393, 572, 615, 672
NmeAIII GCCGAG 2 cut(s) 273, 634
NmuCI GTSAC 1 cut(s) 811
NspI RCATGY 1 cut(s) 416
PaeR7I CTCGAG 1 cut(s) 350
PciI ACATGT 1 cut(s) 412
PcsI WCGNNNNNNNCGW 1 cut(s) 485
PfeI GAWTC 2 cut(s) 166, 793
PkrI GCNGC 9 cut(s) 49, 76, 213, 216, 396, 399, 402, 432, 646
PleI GAGTC 1 cut(s) 296
PluTI GGCGCC 2 cut(s) 395, 617
PmaCI CACGTG 1 cut(s) 688
PmlI CACGTG 1 cut(s) 688
PpsI GAGTC 1 cut(s) 296
Ppu21I YACGTR 2 cut(s) 506, 688
PpuMI RGGWCCY 2 cut(s) 358, 571
PscI ACATGT 1 cut(s) 412
Psp5II RGGWCCY 2 cut(s) 358, 571
Psp6I CCWGG 2 cut(s) 616, 656
PspCI CACGTG 1 cut(s) 688
PspGI CCWGG 2 cut(s) 616, 656
PspN4I GGNNCC 5 cut(s) 360, 393, 572, 615, 672
PspPI GGNCC 5 cut(s) 315, 335, 358, 571, 671
PspPPI RGGWCCY 2 cut(s) 358, 571
PspXI VCTCGAGB 1 cut(s) 350
PstI CTGCAG 1 cut(s) 52
PstNI CAGNNNCTG 1 cut(s) 647
PvuII CAGCTG 2 cut(s) 400, 644
RsaI GTAC 3 cut(s) 411, 464, 557
RsaNI GTAC 3 cut(s) 410, 463, 556
SaqAI TTAA 3 cut(s) 324, 383, 841
SatI GCNGC 9 cut(s) 48, 75, 212, 215, 395, 398, 401, 431, 645
Sau3AI GATC 2 cut(s) 582, 706
Sau96I GGNCC 5 cut(s) 315, 335, 358, 571, 671
SchI GAGTC 1 cut(s) 296
ScrFI CCNGG 3 cut(s) 618, 658, 675
SduI GDGCHC 2 cut(s) 17, 680
SfaNI GCATC 2 cut(s) 16, 577
SfcI CTRYAG 2 cut(s) 48, 173
SfoI GGCGCC 2 cut(s) 393, 615
Sfr274I CTCGAG 1 cut(s) 350
SinI GGWCC 3 cut(s) 358, 571, 671
SlaI CTCGAG 1 cut(s) 350
SmlI CTYRAG 2 cut(s) 350, 434
SmoI CTYRAG 2 cut(s) 350, 434
Sse9I AATT 1 cut(s) 180
SsiI CCGC 5 cut(s) 212, 395, 407, 424, 731
SspDI GGCGCC 2 cut(s) 391, 613
SspMI CTAG 1 cut(s) 44
StyD4I CCNGG 3 cut(s) 616, 656, 673
StyI CCWWGG 1 cut(s) 222
TaaI ACNGT 2 cut(s) 499, 519
TaiI ACGT 4 cut(s) 344, 482, 508, 690
TaqI TCGA 6 cut(s) 202, 351, 585, 622, 680, 709
TasI AATT 1 cut(s) 180
TauI GCSGC 2 cut(s) 214, 397
TfiI GAWTC 2 cut(s) 166, 793
Tru1I TTAA 3 cut(s) 324, 383, 841
Tru9I TTAA 3 cut(s) 324, 383, 841
TscAI CASTG 2 cut(s) 504, 524
TseFI GTSAC 1 cut(s) 811
TseI GCWGC 7 cut(s) 47, 74, 214, 397, 400, 430, 644
Tsp45I GTSAC 1 cut(s) 811
TspDTI ATGAA 2 cut(s) 17, 73
TspGWI ACGGA 1 cut(s) 786
TspRI CASTG 2 cut(s) 504, 524
VpaK11BI GGWCC 3 cut(s) 358, 571, 671
XagI CCTNNNNNAGG 1 cut(s) 353
XceI RCATGY 1 cut(s) 416
XhoI CTCGAG 1 cut(s) 350
XspI CTAG 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.