pycom13g10440

Glucuronoxylan 4-O-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0005005
Physical Location & Seq
Reverse (-)
6986360 .. 6987208
849 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g10440.1

Sequence Viewer

Length: 849 bp
ATGAAGAATCGGAGTCCATTGTCGGAGAGACCCGTGTTTCTCGTCGTCATTCTGGTTCTACTGATCGGCGCGGCGCTGGCGATCTCCAGCTTCTTCCAGGCTGGCACCTCCGTCCTCTACTCCATCGCCGGACCTACATTTCTCCGCGCCGTCGACGATGCCCCGGCGGCCATCCAGCTCCAGGCCATCCTCCACTACGCCACGTCGCACCAGACCCCGCAGCAGTCGCGAGACGAGATCGCTGTATCCTTCGAGGTCCTCAAGTCCCGCTCCCCCTGCAACTTCCTCGTGTTCGGCCTAGGCTTCGACTCCCTCATGTGGGCCTCCCTGAATCCACGAGGCACGACGCTCTTCCTGGAGGAGGACCCCAAGTGGGTCCAGACCGTCCTCAAGGACGCCCCCCAGCTGCGGGCAGAGACGGTGCGCTACCGCACGCAGCTCAAGGAGGCGGACGAGCTCCTCTCCTCGTACCGCTCCCAACGGTCGTGCTCCCCGTCCGAGGCTGTCCTGCGCGGAAATACCGAGTGCAGGCTGGCGCTGGACAACCTGCCGGACGAGGTGTACGACAAGGAGTGGGACCTGGTCATGGTCGATGCGCCGCGCGGGTATTTCCCGGAGGCGCCGGGGCGGATGGGGGCTATATTCTCTGCGGCTGTAATGGCGAGGAGGAGGCGGGGATCCGGTGTGACGCACGTGTTCCTTCACGATGTGGATCGGAAGGTGGAGAAGGCGTTCGCGGAGGAGTTCCTCTGCAGGAAGTTCTTGGTTAAAGCGGTGGGGAGGCTCTGGCACTTTGAGATCCCCTCCGCCGCTAACGTGACGCACGGTGACGGCGAACGTTTTTGCTAA

Protein Analysis

283

Amino Acids

31.54

Weight (kDa)

7.01

Isoelectric Point (pI)

48.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IRX15_IRX15L_GXM PF21729 79 - 265 5.4e-83 IRX15/IRX15L/GXM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 555
AccB1I GGYRCC 2 cut(s) 104, 619
AccBSI CCGCTC 2 cut(s) 270, 474
AccI GTMKAC 1 cut(s) 153
AccII CGCG 7 cut(s) 71, 147, 229, 513, 601, 603, 737
AclI AACGTT 1 cut(s) 838
AclWI GGATC 4 cut(s) 672, 685, 720, 793
AcoI YGGCCR 1 cut(s) 168
AcvI CACGTG 1 cut(s) 694
AcyI GRCGYC 2 cut(s) 396, 620
AdeI CACNNNGTG 1 cut(s) 709
AfaI GTAC 2 cut(s) 470, 563
AflIII ACRYGT 1 cut(s) 693
AjiI CACGTC 1 cut(s) 204
AjnI CCWGG 4 cut(s) 96, 180, 354, 579
AloI GAACNNNNNNTCC 2 cut(s) 716, 748
AluBI AGCT 5 cut(s) 90, 178, 406, 439, 457
AluI AGCT 5 cut(s) 90, 178, 406, 439, 457
Alw21I GWGCWC 2 cut(s) 459, 491
Alw26I GTCTC 3 cut(s) 22, 225, 410
AlwI GGATC 4 cut(s) 672, 685, 720, 793
AoxI GGCC 4 cut(s) 168, 183, 295, 321
ApeKI GCWGC 3 cut(s) 220, 406, 436
Asp700I GAANNNNTTC 1 cut(s) 731
AspA2I CCTAGG 1 cut(s) 298
AspLEI GCGC 9 cut(s) 71, 76, 149, 426, 513, 538, 598, 603, 622
AspS9I GGNCC 6 cut(s) 131, 256, 321, 364, 376, 577
AsuC2I CCSGG 3 cut(s) 164, 614, 624
AsuHPI GGTGA 1 cut(s) 839
AvaII GGWCC 5 cut(s) 131, 256, 364, 376, 577
AvrII CCTAGG 1 cut(s) 298
BamHI GGATCC 1 cut(s) 677
BanI GGYRCC 2 cut(s) 104, 619
BanII GRGCYC 1 cut(s) 459
BauI CACGAG 2 cut(s) 287, 336
BbrPI CACGTG 1 cut(s) 694
Bbv12I GWGCWC 2 cut(s) 459, 491
BbvI GCAGC 3 cut(s) 232, 393, 448
BccI CCATC 4 cut(s) 131, 179, 194, 625
BceAI ACGGC 2 cut(s) 134, 847
BcgI CGANNNNNNTGC 2 cut(s) 268, 302
BciT130I CCWGG 4 cut(s) 98, 182, 356, 581
BciVI GTATCC 1 cut(s) 256
BcnI CCSGG 3 cut(s) 164, 614, 624
BcoDI GTCTC 3 cut(s) 22, 225, 410
BfaI CTAG 1 cut(s) 299
BfmI CTRYAG 1 cut(s) 751
BfoI RGCGCY 3 cut(s) 77, 539, 623
BfuAI ACCTGC 1 cut(s) 555
BfuI GTATCC 1 cut(s) 256
BglI GCCNNNNNGGC 1 cut(s) 167
BisI GCNGC 8 cut(s) 72, 168, 221, 407, 437, 599, 651, 810
BlnI CCTAGG 1 cut(s) 298
BlsI GCNGC 8 cut(s) 73, 169, 222, 408, 438, 600, 652, 811
Bme1390I CCNGG 7 cut(s) 98, 164, 182, 356, 581, 614, 624
Bme18I GGWCC 5 cut(s) 131, 256, 364, 376, 577
BmgBI CACGTC 1 cut(s) 204
BmgT120I GGNCC 6 cut(s) 131, 256, 321, 364, 376, 577
BmiI GGNNCC 6 cut(s) 106, 366, 377, 578, 621, 679
BmrFI CCNGG 7 cut(s) 98, 164, 182, 356, 581, 614, 624
BmsI GCATC 2 cut(s) 148, 583
BplI GAGNNNNNCTC 4 cut(s) 446, 478, 788, 820
BpmI CTGGAG 3 cut(s) 70, 164, 377
BpuEI CTTGAG 3 cut(s) 245, 374, 425
BpuMI CCSGG 3 cut(s) 164, 614, 624
BsaAI YACGTR 1 cut(s) 694
BsaBI GATNNNNATC 1 cut(s) 711
BsaHI GRCGYC 2 cut(s) 396, 620
BsaI GGTCTC 1 cut(s) 22
BsaJI CCNNGG 4 cut(s) 162, 298, 498, 623
BsaWI WCCGGW 1 cut(s) 680
BsaXI ACNNNNNCTCC 2 cut(s) 716, 746
Bse8I GATNNNNATC 1 cut(s) 711
BseBI CCWGG 4 cut(s) 98, 182, 356, 581
BseDI CCNNGG 4 cut(s) 162, 298, 498, 623
BseGI GGATG 3 cut(s) 171, 186, 636
BseJI GATNNNNATC 1 cut(s) 711
BseRI GAGGAG 6 cut(s) 374, 449, 454, 679, 682, 755
BseXI GCAGC 3 cut(s) 232, 393, 448
BseYI CCCAGC 1 cut(s) 402
BsgI GTGCAG 1 cut(s) 547
Bsh1236I CGCG 7 cut(s) 71, 147, 229, 513, 601, 603, 737
Bsh1285I CGRYCG 1 cut(s) 485
BshFI GGCC 4 cut(s) 170, 185, 297, 323
BshNI GGYRCC 2 cut(s) 104, 619
BsiEI CGRYCG 1 cut(s) 485
BsiHKAI GWGCWC 2 cut(s) 459, 491
BsiSI CCGG 6 cut(s) 129, 164, 551, 614, 623, 681
BslFI GGGAC 2 cut(s) 250, 590
BsmAI GTCTC 3 cut(s) 22, 225, 410
BsmBI CGTCTC 2 cut(s) 225, 410
BsmFI GGGAC 2 cut(s) 250, 590
BsnI GGCC 4 cut(s) 170, 185, 297, 323
Bso31I GGTCTC 1 cut(s) 22
Bsp1286I GDGCHC 2 cut(s) 459, 491
Bsp143I GATC 6 cut(s) 63, 81, 237, 677, 712, 798
Bsp68I TCGCGA 1 cut(s) 229
BspANI GGCC 4 cut(s) 170, 185, 297, 323
BspFNI CGCG 7 cut(s) 71, 147, 229, 513, 601, 603, 737
BspLI GGNNCC 6 cut(s) 106, 366, 377, 578, 621, 679
BspMAI CTGCAG 1 cut(s) 755
BspMI ACCTGC 1 cut(s) 555
BspPI GGATC 4 cut(s) 672, 685, 720, 793
BspQI GCTCTTC 1 cut(s) 356
BspT107I GGYRCC 2 cut(s) 104, 619
BspTNI GGTCTC 1 cut(s) 22
BsrBI CCGCTC 2 cut(s) 270, 474
BssECI CCNNGG 4 cut(s) 162, 298, 498, 623
BssMI GATC 6 cut(s) 63, 81, 237, 677, 712, 798
BssNI GRCGYC 2 cut(s) 396, 620
BssSI CACGAG 2 cut(s) 287, 336
BssT1I CCWWGG 1 cut(s) 298
Bst2BI CACGAG 2 cut(s) 287, 336
Bst2UI CCWGG 4 cut(s) 98, 182, 356, 581
Bst4CI ACNGT 4 cut(s) 385, 421, 483, 827
Bst6I CTCTTC 1 cut(s) 356
BstACI GRCGYC 2 cut(s) 396, 620
BstBAI YACGTR 1 cut(s) 694
BstC8I GCNNGC 6 cut(s) 78, 103, 411, 434, 530, 534
BstENI CCTNNNNNAGG 1 cut(s) 359
BstF5I GGATG 3 cut(s) 171, 186, 636
BstFNI CGCG 7 cut(s) 71, 147, 229, 513, 601, 603, 737
BstH2I RGCGCY 3 cut(s) 77, 539, 623
BstHHI GCGC 9 cut(s) 71, 76, 149, 426, 513, 538, 598, 603, 622
BstKTI GATC 6 cut(s) 66, 84, 240, 680, 715, 801
BstMAI GTCTC 3 cut(s) 22, 225, 410
BstMBI GATC 6 cut(s) 63, 81, 237, 677, 712, 798
BstMCI CGRYCG 1 cut(s) 485
BstMWI GCNNNNNNNGC 5 cut(s) 77, 167, 226, 276, 659
BstNI CCWGG 4 cut(s) 98, 182, 356, 581
BstSCI CCNGG 7 cut(s) 96, 162, 180, 354, 579, 612, 622
BstSFI CTRYAG 1 cut(s) 751
BstUI CGCG 7 cut(s) 71, 147, 229, 513, 601, 603, 737
BstV1I GCAGC 3 cut(s) 232, 393, 448
BstX2I RGATCY 2 cut(s) 677, 798
BstYI RGATCY 2 cut(s) 677, 798
BsuI GTATCC 1 cut(s) 256
BsuRI GGCC 4 cut(s) 170, 185, 297, 323
BtgZI GCGATG 1 cut(s) 109
BtrI CACGTC 1 cut(s) 204
BtsCI GGATG 3 cut(s) 171, 186, 636
BtuMI TCGCGA 1 cut(s) 229
BveI ACCTGC 1 cut(s) 555
Cac8I GCNNGC 6 cut(s) 78, 103, 411, 434, 530, 534
CfoI GCGC 9 cut(s) 71, 76, 149, 426, 513, 538, 598, 603, 622
Cfr13I GGNCC 6 cut(s) 131, 256, 321, 364, 376, 577
CseI GACGC 4 cut(s) 355, 404, 697, 829
CsiI ACCWGGT 1 cut(s) 579
Csp6I GTAC 2 cut(s) 469, 562
CviAII CATG 2 cut(s) 316, 586
CviQI GTAC 2 cut(s) 469, 562
DinI GGCGCC 1 cut(s) 621
DpnI GATC 6 cut(s) 65, 83, 239, 679, 714, 800
DpnII GATC 6 cut(s) 63, 81, 237, 677, 712, 798
DraIII CACNNNGTG 1 cut(s) 709
EaeI YGGCCR 1 cut(s) 168
Eam1104I CTCTTC 1 cut(s) 356
EarI CTCTTC 1 cut(s) 356
EciI GGCGGA 3 cut(s) 464, 643, 796
Ecl136II GAGCTC 1 cut(s) 457
Eco130I CCWWGG 1 cut(s) 298
Eco24I GRGCYC 1 cut(s) 459
Eco31I GGTCTC 1 cut(s) 22
Eco47I GGWCC 5 cut(s) 131, 256, 364, 376, 577
Eco53kI GAGCTC 1 cut(s) 457
Eco72I CACGTG 1 cut(s) 694
EcoICRI GAGCTC 1 cut(s) 457
EcoNI CCTNNNNNAGG 1 cut(s) 359
EcoO109I RGGNCCY 3 cut(s) 256, 364, 577
EcoRII CCWGG 4 cut(s) 96, 180, 354, 579
EcoT14I CCWWGG 1 cut(s) 298
EcoT38I GRGCYC 1 cut(s) 459
EgeI GGCGCC 1 cut(s) 621
EheI GGCGCC 1 cut(s) 621
ErhI CCWWGG 1 cut(s) 298
Esp3I CGTCTC 2 cut(s) 225, 410
FaeI CATG 2 cut(s) 319, 589
FaiI YATR 3 cut(s) 317, 587, 641
FaqI GGGAC 2 cut(s) 250, 590
FatI CATG 2 cut(s) 315, 585
FauI CCCGC 5 cut(s) 225, 275, 402, 596, 666
FblI GTMKAC 1 cut(s) 153
Fnu4HI GCNGC 8 cut(s) 72, 168, 221, 407, 437, 599, 651, 810
FokI GGATG 3 cut(s) 158, 173, 643
FriOI GRGCYC 1 cut(s) 459
Fsp4HI GCNGC 8 cut(s) 72, 168, 221, 407, 437, 599, 651, 810
FspBI CTAG 1 cut(s) 299
GlaI GCGC 9 cut(s) 70, 75, 148, 425, 512, 537, 597, 602, 621
GluI GCNGC 8 cut(s) 72, 168, 221, 407, 437, 599, 651, 810
GsaI CCCAGC 1 cut(s) 406
GsuI CTGGAG 3 cut(s) 70, 164, 377
HaeII RGCGCY 3 cut(s) 77, 539, 623
HaeIII GGCC 4 cut(s) 170, 185, 297, 323
HapII CCGG 6 cut(s) 129, 164, 551, 614, 623, 681
HgaI GACGC 4 cut(s) 355, 404, 697, 829
HhaI GCGC 9 cut(s) 71, 76, 149, 426, 513, 538, 598, 603, 622
Hin1I GRCGYC 2 cut(s) 396, 620
Hin1II CATG 2 cut(s) 319, 589
Hin6I GCGC 9 cut(s) 69, 74, 147, 424, 511, 536, 596, 601, 620
HinP1I GCGC 9 cut(s) 69, 74, 147, 424, 511, 536, 596, 601, 620
HincII GTYRAC 1 cut(s) 154
HindII GTYRAC 1 cut(s) 154
HinfI GANTC 4 cut(s) 7, 13, 308, 331
HpaII CCGG 6 cut(s) 129, 164, 551, 614, 623, 681
HphI GGTGA 1 cut(s) 839
Hpy166II GTNNAC 2 cut(s) 154, 562
Hpy188I TCNGA 4 cut(s) 12, 25, 499, 717
Hpy188III TCNNGA 3 cut(s) 228, 379, 704
Hpy8I GTNNAC 2 cut(s) 154, 562
Hpy99I CGWCG 5 cut(s) 47, 155, 158, 208, 349
HpyAV CCTTC 4 cut(s) 259, 710, 712, 721
HpyCH4III ACNGT 4 cut(s) 385, 421, 483, 827
HpyCH4IV ACGT 4 cut(s) 203, 693, 816, 838
HpyCH4V TGCA 3 cut(s) 279, 528, 753
HpyF10VI GCNNNNNNNGC 5 cut(s) 77, 167, 226, 276, 659
HpySE526I ACGT 4 cut(s) 203, 693, 816, 838
Hsp92I GRCGYC 2 cut(s) 396, 620
Hsp92II CATG 2 cut(s) 319, 589
HspAI GCGC 9 cut(s) 69, 74, 147, 424, 511, 536, 596, 601, 620
KasI GGCGCC 1 cut(s) 619
Kzo9I GATC 6 cut(s) 63, 81, 237, 677, 712, 798
LguI GCTCTTC 1 cut(s) 356
LmnI GCTCC 5 cut(s) 183, 275, 462, 479, 494
Lsp1109I GCAGC 3 cut(s) 232, 393, 448
LweI GCATC 2 cut(s) 148, 583
MabI ACCWGGT 1 cut(s) 579
MaeI CTAG 1 cut(s) 299
MaeII ACGT 4 cut(s) 203, 693, 816, 838
MaeIII GTNAC 3 cut(s) 685, 817, 827
MalI GATC 6 cut(s) 65, 83, 239, 679, 714, 800
MbiI CCGCTC 2 cut(s) 270, 474
MboI GATC 6 cut(s) 63, 81, 237, 677, 712, 798
MboII GAAGA 3 cut(s) 16, 85, 343
MflI RGATCY 2 cut(s) 677, 798
MhlI GDGCHC 2 cut(s) 459, 491
Mly113I GGCGCC 1 cut(s) 620
MlyI GAGTC 2 cut(s) 22, 302
MroXI GAANNNNTTC 1 cut(s) 731
MseI TTAA 1 cut(s) 768
MspA1I CMGCKG 1 cut(s) 406
MspI CCGG 6 cut(s) 129, 164, 551, 614, 623, 681
MspR9I CCNGG 7 cut(s) 98, 164, 182, 356, 581, 614, 624
MteI GCGCNGCGC 2 cut(s) 72, 599
MvaI CCWGG 4 cut(s) 98, 182, 356, 581
MvnI CGCG 7 cut(s) 71, 147, 229, 513, 601, 603, 737
MwoI GCNNNNNNNGC 5 cut(s) 77, 167, 226, 276, 659
NarI GGCGCC 1 cut(s) 620
NciI CCSGG 3 cut(s) 164, 614, 624
NdeII GATC 6 cut(s) 63, 81, 237, 677, 712, 798
NlaIII CATG 2 cut(s) 319, 589
NlaIV GGNNCC 6 cut(s) 106, 366, 377, 578, 621, 679
NmuCI GTSAC 3 cut(s) 685, 817, 827
NruI TCGCGA 1 cut(s) 229
PciSI GCTCTTC 1 cut(s) 356
PcsI WCGNNNNNNNCGW 3 cut(s) 491, 561, 831
PdmI GAANNNNTTC 1 cut(s) 731
PfeI GAWTC 2 cut(s) 7, 331
PflFI GACNNNGTC 1 cut(s) 581
PfoI TCCNGGA 2 cut(s) 354, 612
PkrI GCNGC 8 cut(s) 73, 169, 222, 408, 438, 600, 652, 811
PleI GAGTC 2 cut(s) 21, 302
PluTI GGCGCC 1 cut(s) 623
PmaCI CACGTG 1 cut(s) 694
PmlI CACGTG 1 cut(s) 694
PpsI GAGTC 2 cut(s) 21, 302
Ppu21I YACGTR 1 cut(s) 694
PpuMI RGGWCCY 3 cut(s) 256, 364, 577
Psp124BI GAGCTC 1 cut(s) 459
Psp1406I AACGTT 1 cut(s) 838
Psp5II RGGWCCY 3 cut(s) 256, 364, 577
Psp6I CCWGG 4 cut(s) 96, 180, 354, 579
PspCI CACGTG 1 cut(s) 694
PspFI CCCAGC 1 cut(s) 402
PspGI CCWGG 4 cut(s) 96, 180, 354, 579
PspN4I GGNNCC 6 cut(s) 106, 366, 377, 578, 621, 679
PspPI GGNCC 6 cut(s) 131, 256, 321, 364, 376, 577
PspPPI RGGWCCY 3 cut(s) 256, 364, 577
PstI CTGCAG 1 cut(s) 755
PsuI RGATCY 2 cut(s) 677, 798
PsyI GACNNNGTC 1 cut(s) 581
PvuII CAGCTG 1 cut(s) 406
RruI TCGCGA 1 cut(s) 229
RsaI GTAC 2 cut(s) 470, 563
RsaNI GTAC 2 cut(s) 469, 562
SacI GAGCTC 1 cut(s) 459
SalI GTCGAC 1 cut(s) 152
SapI GCTCTTC 1 cut(s) 356
SaqAI TTAA 1 cut(s) 768
SatI GCNGC 8 cut(s) 72, 168, 221, 407, 437, 599, 651, 810
Sau3AI GATC 6 cut(s) 63, 81, 237, 677, 712, 798
Sau96I GGNCC 6 cut(s) 131, 256, 321, 364, 376, 577
SchI GAGTC 2 cut(s) 22, 302
ScrFI CCNGG 7 cut(s) 98, 164, 182, 356, 581, 614, 624
SduI GDGCHC 2 cut(s) 459, 491
SexAI ACCWGGT 1 cut(s) 579
SfaNI GCATC 2 cut(s) 148, 583
SfcI CTRYAG 1 cut(s) 751
SfoI GGCGCC 1 cut(s) 621
SgrDI CGTCGACG 1 cut(s) 152
SinI GGWCC 5 cut(s) 131, 256, 364, 376, 577
SmlI CTYRAG 3 cut(s) 260, 389, 440
SmoI CTYRAG 3 cut(s) 260, 389, 440
SspDI GGCGCC 1 cut(s) 619
SspMI CTAG 1 cut(s) 299
SstI GAGCTC 1 cut(s) 459
StyD4I CCNGG 7 cut(s) 96, 162, 180, 354, 579, 612, 622
StyI CCWWGG 1 cut(s) 298
TaaI ACNGT 4 cut(s) 385, 421, 483, 827
TaiI ACGT 4 cut(s) 206, 696, 819, 841
TaqI TCGA 4 cut(s) 153, 252, 306, 591
TauI GCSGC 5 cut(s) 74, 170, 601, 653, 812
TfiI GAWTC 2 cut(s) 7, 331
Tru1I TTAA 1 cut(s) 768
Tru9I TTAA 1 cut(s) 768
TseFI GTSAC 3 cut(s) 685, 817, 827
TseI GCWGC 3 cut(s) 220, 406, 436
Tsp45I GTSAC 3 cut(s) 685, 817, 827
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 100
Tth111I GACNNNGTC 1 cut(s) 581
VpaK11BI GGWCC 5 cut(s) 131, 256, 364, 376, 577
XagI CCTNNNNNAGG 1 cut(s) 359
XmaJI CCTAGG 1 cut(s) 298
XmiI GTMKAC 1 cut(s) 153
XmnI GAANNNNTTC 1 cut(s) 731
XspI CTAG 1 cut(s) 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.