pycom16g10250

Glucuronoxylan 4-O-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0005005
Physical Location & Seq
Reverse (-)
6991076 .. 6991930
855 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g10250.1

Sequence Viewer

Length: 855 bp
ATGAAGAATCGGAGTCCATTTTCCGAGAGACCTGGGTTTCTCGCCGTGACTCTGGTTCTATTGATCGGCGCGGCGCTGGCCATCTCCAGCTTCTGCCAGGGTGGCACCTCCGTCTTCTACTCCATCGCCGGCCCCACATTTCTACGCGCCGTCGACAATAACTACTCGCCGGTGGCGATCCAGATCCAGGCCATCCTCCACTACGCCACGTCGCGCCAGACCCCGCAGCAGTCGCGAAACGAGATCGGCGTGTCCTTCCAGGTCCTCAAGTCCCGCGCCCCCTGCAACTTCCTGGTGTTCGGCCTCGGCTTCGACTCCCTCATGTGGGCCTCCCTGAATCCACGCGGCACGACGCTGTTCCTGGAGGAGGATCCCAAGTGGGTCCAAAGCGTCCTCAAGGACGCCCCTCAGCTGCGGGCCCAGACGGTGCGCTACCGCACGCATCTCAAGGAGGCGGACCAACTCCTTTCCTCGTACCGCTCCAAACGGTCGTGCTCCCCGTCCGAGGCGGTCCTGCGTGGAAATACGGAGTGCAGGCTGGCGCTGGACAACCTGCCGGACGAGGTGTACGACAAAGAGTGGGACCTGATCATGATCGACGCGCCGCGCGGGTATTTCCCGGAGGCGCCGGGCCGGATGGGGGCTATTTTCTCTGCGGCTGTCATGGCGAGGAGGAGGCAGGGATCCGGTGTGACGCACGTGTTCCTGCACGATGTGAATCGGAAGGTGGAGAAGGCGTTCGCGAATGAGTTCTTGTGCAGGAAGTTTTTGGTTAAGGCGGTGGGGAGGCTGTGGCACTTCGAGATCCCCTCCTCCGCAAACGCGACGCACGGTGACGGCTCGCGTTTTTGCTAA

Protein Analysis

285

Amino Acids

31.69

Weight (kDa)

9.4

Isoelectric Point (pI)

50.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IRX15_IRX15L_GXM PF21729 81 - 267 6.4e-83 IRX15/IRX15L/GXM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 561
AccB1I GGYRCC 2 cut(s) 104, 625
AccBSI CCGCTC 1 cut(s) 480
AccI GTMKAC 1 cut(s) 153
AclWI GGATC 7 cut(s) 172, 178, 365, 378, 678, 691, 799
AcoI YGGCCR 1 cut(s) 78
AcvI CACGTG 1 cut(s) 700
AcyI GRCGYC 2 cut(s) 402, 626
AdeI CACNNNGTG 1 cut(s) 715
AfaI GTAC 2 cut(s) 476, 569
AfiI CCNNNNNNNGG 6 cut(s) 187, 324, 325, 367, 505, 640
AflIII ACRYGT 1 cut(s) 699
AjiI CACGTC 1 cut(s) 210
AjnI CCWGG 6 cut(s) 31, 96, 186, 258, 291, 360
AloI GAACNNNNNNTCC 2 cut(s) 722, 754
AluBI AGCT 2 cut(s) 90, 412
AluI AGCT 2 cut(s) 90, 412
Alw21I GWGCWC 1 cut(s) 497
Alw26I GTCTC 1 cut(s) 22
AlwI GGATC 7 cut(s) 172, 178, 365, 378, 678, 691, 799
AlwNI CAGNNNCTG 1 cut(s) 93
AoxI GGCC 7 cut(s) 78, 130, 189, 301, 327, 417, 631
ApaI GGGCCC 1 cut(s) 421
ApeKI GCWGC 2 cut(s) 226, 412
Asp700I GAANNNNTTC 2 cut(s) 737, 749
AsuC2I CCSGG 2 cut(s) 620, 630
AsuHPI GGTGA 1 cut(s) 845
AvaII GGWCC 5 cut(s) 262, 382, 457, 511, 583
BaeGI GKGCMC 1 cut(s) 421
BalI TGGCCA 1 cut(s) 80
BamHI GGATCC 2 cut(s) 370, 683
BanI GGYRCC 2 cut(s) 104, 625
BanII GRGCYC 1 cut(s) 421
BbrPI CACGTG 1 cut(s) 700
BbsI GAAGAC 1 cut(s) 106
Bbv12I GWGCWC 1 cut(s) 497
BbvCI CCTCAGC 1 cut(s) 408
BbvI GCAGC 2 cut(s) 238, 399
BccI CCATC 4 cut(s) 89, 131, 200, 631
BceAI ACGGC 3 cut(s) 29, 134, 853
BciT130I CCWGG 6 cut(s) 33, 98, 188, 260, 293, 362
BclI TGATCA 1 cut(s) 588
BcnI CCSGG 2 cut(s) 620, 630
BcoDI GTCTC 1 cut(s) 22
BfoI RGCGCY 3 cut(s) 77, 545, 629
BfuAI ACCTGC 1 cut(s) 561
BglI GCCNNNNNGGC 1 cut(s) 102
BisI GCNGC 6 cut(s) 72, 227, 346, 413, 605, 657
BlsI GCNGC 6 cut(s) 73, 228, 347, 414, 606, 658
Bme1390I CCNGG 8 cut(s) 33, 98, 188, 260, 293, 362, 620, 630
Bme18I GGWCC 5 cut(s) 262, 382, 457, 511, 583
BmgBI CACGTC 1 cut(s) 210
BmiI GGNNCC 8 cut(s) 106, 133, 372, 383, 419, 584, 627, 685
BmrFI CCNGG 8 cut(s) 33, 98, 188, 260, 293, 362, 620, 630
BmsI GCATC 1 cut(s) 451
BpiI GAAGAC 1 cut(s) 106
BplI GAGNNNNNCTC 2 cut(s) 794, 826
BpmI CTGGAG 2 cut(s) 70, 383
Bpu10I CCTNAGC 1 cut(s) 408
BpuEI CTTGAG 3 cut(s) 251, 380, 431
BpuMI CCSGG 2 cut(s) 620, 630
BsaAI YACGTR 1 cut(s) 700
BsaBI GATNNNNATC 3 cut(s) 182, 593, 717
BsaHI GRCGYC 2 cut(s) 402, 626
BsaI GGTCTC 1 cut(s) 22
BsaJI CCNNGG 4 cut(s) 32, 97, 304, 504
BsaWI WCCGGW 1 cut(s) 686
BsaXI ACNNNNNCTCC 2 cut(s) 722, 752
Bsc4I CCNNNNNNNGG 6 cut(s) 187, 324, 325, 367, 505, 640
Bse118I RCCGGY 2 cut(s) 128, 169
Bse8I GATNNNNATC 3 cut(s) 182, 593, 717
BseBI CCWGG 6 cut(s) 33, 98, 188, 260, 293, 362
BseDI CCNNGG 4 cut(s) 32, 97, 304, 504
BseGI GGATG 2 cut(s) 192, 642
BseJI GATNNNNATC 3 cut(s) 182, 593, 717
BseLI CCNNNNNNNGG 6 cut(s) 187, 324, 325, 367, 505, 640
BseMII CTCAG 1 cut(s) 422
BseRI GAGGAG 4 cut(s) 380, 685, 688, 802
BseSI GKGCMC 1 cut(s) 421
BseXI GCAGC 2 cut(s) 238, 399
BsgI GTGCAG 3 cut(s) 553, 692, 778
Bsh1285I CGRYCG 1 cut(s) 491
BshFI GGCC 7 cut(s) 80, 132, 191, 303, 329, 419, 633
BshNI GGYRCC 2 cut(s) 104, 625
BsiEI CGRYCG 1 cut(s) 491
BsiHKAI GWGCWC 1 cut(s) 497
BsiSI CCGG 7 cut(s) 129, 170, 557, 620, 629, 634, 687
BslFI GGGAC 2 cut(s) 256, 596
BslI CCNNNNNNNGG 6 cut(s) 187, 324, 325, 367, 505, 640
BsmAI GTCTC 1 cut(s) 22
BsmFI GGGAC 2 cut(s) 256, 596
BsnI GGCC 7 cut(s) 80, 132, 191, 303, 329, 419, 633
Bso31I GGTCTC 1 cut(s) 22
Bsp120I GGGCCC 1 cut(s) 417
Bsp1286I GDGCHC 2 cut(s) 421, 497
Bsp143I GATC 9 cut(s) 63, 177, 183, 243, 370, 588, 594, 683, 804
Bsp68I TCGCGA 2 cut(s) 235, 743
BspANI GGCC 7 cut(s) 80, 132, 191, 303, 329, 419, 633
BspCNI CTCAG 1 cut(s) 421
BspHI TCATGA 1 cut(s) 591
BspLI GGNNCC 8 cut(s) 106, 133, 372, 383, 419, 584, 627, 685
BspMI ACCTGC 1 cut(s) 561
BspPI GGATC 7 cut(s) 172, 178, 365, 378, 678, 691, 799
BspT107I GGYRCC 2 cut(s) 104, 625
BspTNI GGTCTC 1 cut(s) 22
BsrBI CCGCTC 1 cut(s) 480
BsrFI RCCGGY 2 cut(s) 128, 169
BssAI RCCGGY 2 cut(s) 128, 169
BssECI CCNNGG 4 cut(s) 32, 97, 304, 504
BssMI GATC 9 cut(s) 63, 177, 183, 243, 370, 588, 594, 683, 804
BssNI GRCGYC 2 cut(s) 402, 626
Bst2UI CCWGG 6 cut(s) 33, 98, 188, 260, 293, 362
Bst4CI ACNGT 3 cut(s) 427, 489, 833
BstACI GRCGYC 2 cut(s) 402, 626
BstBAI YACGTR 1 cut(s) 700
BstC8I GCNNGC 7 cut(s) 78, 130, 417, 440, 536, 540, 842
BstDEI CTNAG 1 cut(s) 408
BstENI CCTNNNNNAGG 1 cut(s) 365
BstF5I GGATG 2 cut(s) 192, 642
BstH2I RGCGCY 3 cut(s) 77, 545, 629
BstKTI GATC 9 cut(s) 66, 180, 186, 246, 373, 591, 597, 686, 807
BstMAI GTCTC 1 cut(s) 22
BstMBI GATC 9 cut(s) 63, 177, 183, 243, 370, 588, 594, 683, 804
BstMCI CGRYCG 1 cut(s) 491
BstMWI GCNNNNNNNGC 5 cut(s) 77, 102, 232, 282, 665
BstNI CCWGG 6 cut(s) 33, 98, 188, 260, 293, 362
BstSCI CCNGG 8 cut(s) 31, 96, 186, 258, 291, 360, 618, 628
BstSLI GKGCMC 1 cut(s) 421
BstV1I GCAGC 2 cut(s) 238, 399
BstV2I GAAGAC 1 cut(s) 106
BstX2I RGATCY 4 cut(s) 183, 370, 683, 804
BstYI RGATCY 4 cut(s) 183, 370, 683, 804
BsuRI GGCC 7 cut(s) 80, 132, 191, 303, 329, 419, 633
BtgZI GCGATG 1 cut(s) 109
BtrI CACGTC 1 cut(s) 210
BtsCI GGATG 2 cut(s) 192, 642
BtuMI TCGCGA 2 cut(s) 235, 743
BveI ACCTGC 1 cut(s) 561
Cac8I GCNNGC 7 cut(s) 78, 130, 417, 440, 536, 540, 842
CaiI CAGNNNCTG 1 cut(s) 93
CciI TCATGA 1 cut(s) 591
Cfr10I RCCGGY 2 cut(s) 128, 169
CseI GACGC 6 cut(s) 361, 379, 410, 608, 703, 835
Csp6I GTAC 2 cut(s) 475, 568
CviAII CATG 3 cut(s) 322, 592, 664
CviQI GTAC 2 cut(s) 475, 568
DdeI CTNAG 1 cut(s) 408
DinI GGCGCC 1 cut(s) 627
DpnI GATC 9 cut(s) 65, 179, 185, 245, 372, 590, 596, 685, 806
DpnII GATC 9 cut(s) 63, 177, 183, 243, 370, 588, 594, 683, 804
DraIII CACNNNGTG 1 cut(s) 715
EaeI YGGCCR 1 cut(s) 78
EciI GGCGGA 1 cut(s) 470
Eco24I GRGCYC 1 cut(s) 421
Eco31I GGTCTC 1 cut(s) 22
Eco47I GGWCC 5 cut(s) 262, 382, 457, 511, 583
Eco72I CACGTG 1 cut(s) 700
EcoNI CCTNNNNNAGG 1 cut(s) 365
EcoO109I RGGNCCY 2 cut(s) 262, 583
EcoRII CCWGG 6 cut(s) 31, 96, 186, 258, 291, 360
EcoT38I GRGCYC 1 cut(s) 421
EgeI GGCGCC 1 cut(s) 627
EheI GGCGCC 1 cut(s) 627
FaeI CATG 3 cut(s) 325, 595, 667
FaiI YATR 3 cut(s) 323, 593, 665
FaqI GGGAC 2 cut(s) 256, 596
FatI CATG 3 cut(s) 321, 591, 663
FauI CCCGC 4 cut(s) 231, 281, 408, 602
FbaI TGATCA 1 cut(s) 588
FblI GTMKAC 1 cut(s) 153
Fnu4HI GCNGC 6 cut(s) 72, 227, 346, 413, 605, 657
FokI GGATG 2 cut(s) 179, 649
FriOI GRGCYC 1 cut(s) 421
Fsp4HI GCNGC 6 cut(s) 72, 227, 346, 413, 605, 657
GluI GCNGC 6 cut(s) 72, 227, 346, 413, 605, 657
GsuI CTGGAG 2 cut(s) 70, 383
HaeII RGCGCY 3 cut(s) 77, 545, 629
HaeIII GGCC 7 cut(s) 80, 132, 191, 303, 329, 419, 633
HapII CCGG 7 cut(s) 129, 170, 557, 620, 629, 634, 687
HgaI GACGC 6 cut(s) 361, 379, 410, 608, 703, 835
Hin1I GRCGYC 2 cut(s) 402, 626
Hin1II CATG 3 cut(s) 325, 595, 667
HincII GTYRAC 1 cut(s) 154
HindII GTYRAC 1 cut(s) 154
HinfI GANTC 6 cut(s) 7, 13, 49, 314, 337, 718
HpaII CCGG 7 cut(s) 129, 170, 557, 620, 629, 634, 687
HphI GGTGA 1 cut(s) 845
Hpy166II GTNNAC 2 cut(s) 154, 568
Hpy188I TCNGA 4 cut(s) 12, 25, 505, 723
Hpy188III TCNNGA 5 cut(s) 181, 234, 592, 742, 802
Hpy8I GTNNAC 2 cut(s) 154, 568
Hpy99I CGWCG 5 cut(s) 155, 214, 355, 602, 829
HpyAV CCTTC 3 cut(s) 265, 718, 727
HpyCH4III ACNGT 3 cut(s) 427, 489, 833
HpyCH4IV ACGT 2 cut(s) 209, 699
HpyCH4V TGCA 4 cut(s) 285, 534, 709, 759
HpyF10VI GCNNNNNNNGC 5 cut(s) 77, 102, 232, 282, 665
HpyF3I CTNAG 1 cut(s) 408
HpySE526I ACGT 2 cut(s) 209, 699
Hsp92I GRCGYC 2 cut(s) 402, 626
Hsp92II CATG 3 cut(s) 325, 595, 667
KasI GGCGCC 1 cut(s) 625
KroI GCCGGC 1 cut(s) 128
KroNI GCCGGC 1 cut(s) 130
Ksp22I TGATCA 1 cut(s) 588
Kzo9I GATC 9 cut(s) 63, 177, 183, 243, 370, 588, 594, 683, 804
LmnI GCTCC 2 cut(s) 485, 500
Lsp1109I GCAGC 2 cut(s) 238, 399
LweI GCATC 1 cut(s) 451
MaeII ACGT 2 cut(s) 209, 699
MaeIII GTNAC 3 cut(s) 46, 691, 833
MalI GATC 9 cut(s) 65, 179, 185, 245, 372, 590, 596, 685, 806
MbiI CCGCTC 1 cut(s) 480
MboI GATC 9 cut(s) 63, 177, 183, 243, 370, 588, 594, 683, 804
MboII GAAGA 2 cut(s) 16, 106
MflI RGATCY 4 cut(s) 183, 370, 683, 804
MhlI GDGCHC 2 cut(s) 421, 497
MlsI TGGCCA 1 cut(s) 80
MluNI TGGCCA 1 cut(s) 80
Mly113I GGCGCC 1 cut(s) 626
MlyI GAGTC 3 cut(s) 22, 43, 308
Mox20I TGGCCA 1 cut(s) 80
MroNI GCCGGC 1 cut(s) 128
MroXI GAANNNNTTC 2 cut(s) 737, 749
MscI TGGCCA 1 cut(s) 80
MseI TTAA 1 cut(s) 774
Msp20I TGGCCA 1 cut(s) 80
MspA1I CMGCKG 1 cut(s) 412
MspI CCGG 7 cut(s) 129, 170, 557, 620, 629, 634, 687
MspR9I CCNGG 8 cut(s) 33, 98, 188, 260, 293, 362, 620, 630
MteI GCGCNGCGC 2 cut(s) 72, 605
MvaI CCWGG 6 cut(s) 33, 98, 188, 260, 293, 362
MwoI GCNNNNNNNGC 5 cut(s) 77, 102, 232, 282, 665
NaeI GCCGGC 1 cut(s) 130
NarI GGCGCC 1 cut(s) 626
NciI CCSGG 2 cut(s) 620, 630
NdeII GATC 9 cut(s) 63, 177, 183, 243, 370, 588, 594, 683, 804
NgoMIV GCCGGC 1 cut(s) 128
NlaIII CATG 3 cut(s) 325, 595, 667
NlaIV GGNNCC 8 cut(s) 106, 133, 372, 383, 419, 584, 627, 685
NmeAIII GCCGAG 1 cut(s) 285
NmuCI GTSAC 3 cut(s) 46, 691, 833
NruI TCGCGA 2 cut(s) 235, 743
PagI TCATGA 1 cut(s) 591
PcsI WCGNNNNNNNCGW 4 cut(s) 173, 246, 497, 567
PdiI GCCGGC 1 cut(s) 130
PdmI GAANNNNTTC 2 cut(s) 737, 749
PfeI GAWTC 3 cut(s) 7, 337, 718
PfoI TCCNGGA 2 cut(s) 360, 618
PkrI GCNGC 6 cut(s) 73, 228, 347, 414, 606, 658
PleI GAGTC 3 cut(s) 21, 43, 308
PluTI GGCGCC 1 cut(s) 629
PmaCI CACGTG 1 cut(s) 700
PmlI CACGTG 1 cut(s) 700
PpsI GAGTC 3 cut(s) 21, 43, 308
Ppu21I YACGTR 1 cut(s) 700
PpuMI RGGWCCY 2 cut(s) 262, 583
Psp5II RGGWCCY 2 cut(s) 262, 583
Psp6I CCWGG 6 cut(s) 31, 96, 186, 258, 291, 360
PspCI CACGTG 1 cut(s) 700
PspGI CCWGG 6 cut(s) 31, 96, 186, 258, 291, 360
PspN4I GGNNCC 8 cut(s) 106, 133, 372, 383, 419, 584, 627, 685
PspOMI GGGCCC 1 cut(s) 417
PspPPI RGGWCCY 2 cut(s) 262, 583
PstNI CAGNNNCTG 1 cut(s) 93
PsuI RGATCY 4 cut(s) 183, 370, 683, 804
PvuII CAGCTG 1 cut(s) 412
RruI TCGCGA 2 cut(s) 235, 743
RsaI GTAC 2 cut(s) 476, 569
RsaNI GTAC 2 cut(s) 475, 568
SalI GTCGAC 1 cut(s) 152
SaqAI TTAA 1 cut(s) 774
SatI GCNGC 6 cut(s) 72, 227, 346, 413, 605, 657
Sau3AI GATC 9 cut(s) 63, 177, 183, 243, 370, 588, 594, 683, 804
SchI GAGTC 3 cut(s) 22, 43, 308
ScrFI CCNGG 8 cut(s) 33, 98, 188, 260, 293, 362, 620, 630
SduI GDGCHC 2 cut(s) 421, 497
SfaNI GCATC 1 cut(s) 451
SfoI GGCGCC 1 cut(s) 627
SgrAI CRCCGGYG 1 cut(s) 169
SinI GGWCC 5 cut(s) 262, 382, 457, 511, 583
SmlI CTYRAG 3 cut(s) 266, 395, 446
SmoI CTYRAG 3 cut(s) 266, 395, 446
SspDI GGCGCC 1 cut(s) 625
StyD4I CCNGG 8 cut(s) 31, 96, 186, 258, 291, 360, 618, 628
TaaI ACNGT 3 cut(s) 427, 489, 833
TaiI ACGT 2 cut(s) 212, 702
TaqI TCGA 4 cut(s) 153, 312, 597, 801
TauI GCSGC 4 cut(s) 74, 348, 607, 659
TfiI GAWTC 3 cut(s) 7, 337, 718
Tru1I TTAA 1 cut(s) 774
Tru9I TTAA 1 cut(s) 774
TseFI GTSAC 3 cut(s) 46, 691, 833
TseI GCWGC 2 cut(s) 226, 412
Tsp45I GTSAC 3 cut(s) 46, 691, 833
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 100, 542
VpaK11BI GGWCC 5 cut(s) 262, 382, 457, 511, 583
XagI CCTNNNNNAGG 1 cut(s) 365
XmiI GTMKAC 1 cut(s) 153
XmnI GAANNNNTTC 2 cut(s) 737, 749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.