AT1G70100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003792
Physical Location & Seq
Forward (+)
26402978 .. 26406190
3213 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G70100.2

Sequence Viewer

Length: 1404 bp
ATGGTGGATATGGAAGTTGGTATTGCCAGTGGTGAAGATAAGATAACGACCGCGGCTTCATCGAATCAAGAACTGCAAGTGTCTGTTTCGTTTGGTAAGTTTGAGAATGATTCACTTTCATGGGAGAAGTTTTCATCTTTTTCTCCAAACAAGTATTTGGAAGAAGTGGAGAAGTGTGCAACTGCTGGATCAGTAGCTCAGAAGAAGGCTTATTTCGAATCACATTACAAGAAGATTGCTGAGAGGAGAGCTGATATCATCATGGAGCAGGAGAAACTTTTGGAGAGGAATGCATCTTTCAGGCCGAATATTCAGAACCGGGAAAGAACAGACGATAGTGACAATGACGAGTCAATGATGATCGAGTTTAGTACCGGTTATGGATCTAACGGTGAAAGTACTTCAGAAGAAGATAAGCTTGTGACCGTCATTGTTACTGAAGTGAATGAGACATGTAACCATAAGCCTCTTGAAGAAACTATGGATTTCAAGGAATGTCGAAGCTCGGTTGATACTGGGGATGATCTGAGTACCCTGAAGTTGGAGGAGAAACTTGAAGAGATTGTTCAAGTGGAGGATAAAGAGAAAGTAGAAGAGGTTATTTGTATGAAGGAAGAAGTAAAAGAAGATGTTCCATCAAAGGATACTGGAGAAATGAGTGAAACACTAATGAAGGAGACGAAGAAAGAGAAAGATCATAATCCAATCAAGAAGACTGATAAAAATGTGCGAACTAACCATATGAGAGCCTCTCCCAAGTCTAACCAGGTGACGAAGAAACCGGTAACGAGCAAGGTTGTAAGTGGTAGGAAAACTCAACCAAGCAAGGAAAAAAGCATGACAAAGGCAACAAATAAAGCAGCATCACCTGTTTTAAAACCTCCAGGGTTTTCAACTCCAAGAGTGTCTAAGTCAGCTTCAACAATAAGTTCAATGTCTACTTCTCGCTCTTCAGTAAAGAAGGAAAGTGTCTCAACTTTACTGAGAAAGAAACAAACCGCTCCAAAGTCATTGCCCATTTCTCTCAATGTGGATCAGTCGGTCTCCGATCCTACTGCTGTTCCAACAACAAGAAAATCGTTGATCATGGAACGAATGGGAGATAAAGACATCGTTAGACGTGCATTTAAGAGTTTTCAGAAGAGTTTCGACCAAATGAAATCTACTGATGATGGGCAAGATACAGCTCCAAAGCAGGTTCTTGCAAAGGCAACTGCTGTTCAGAGGTTAGGTACAACTGGTCAGAAAAATGTCAGGCTTGCAAAATCAGATGGCACGGAGAGAAAAGGTCCTAACTCTCACCGTTCTTCATCTGTTGTATCAAAAAGCAACGGGACAGCCGAGAAACAGAAGGACGTAAGATGTTTTATGAGCAAGCCAAACCATGTTCACCTTATCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

467

Amino Acids

51.99

Weight (kDa)

8.84

Isoelectric Point (pI)

39.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1186
AccBSI CCGCTC 1 cut(s) 1001
AccI GTMKAC 1 cut(s) 938
AccII CGCG 1 cut(s) 53
AciI CCGC 3 cut(s) 51, 53, 999
AclWI GGATC 4 cut(s) 196, 391, 1041, 1043
AcuI CTGAAG 4 cut(s) 387, 459, 557, 936
AfaI GTAC 4 cut(s) 373, 400, 532, 1234
AfiI CCNNNNNNNGG 1 cut(s) 541
AflIII ACRYGT 1 cut(s) 452
AgeI ACCGGT 2 cut(s) 374, 781
AgsI TTSAA 7 cut(s) 473, 490, 557, 569, 894, 921, 933
AjiI CACGTC 1 cut(s) 1121
AjnI CCWGG 2 cut(s) 765, 883
AluBI AGCT 6 cut(s) 197, 251, 418, 504, 917, 1187
AluI AGCT 6 cut(s) 197, 251, 418, 504, 917, 1187
Alw26I GTCTC 4 cut(s) 443, 671, 976, 1048
AlwI GGATC 4 cut(s) 196, 391, 1041, 1043
AoxI GGCC 1 cut(s) 302
ApeKI GCWGC 1 cut(s) 860
AsiGI ACCGGT 2 cut(s) 374, 781
Asp700I GAANNNNTTC 2 cut(s) 630, 1145
AspS9I GGNCC 1 cut(s) 1289
AsuC2I CCSGG 1 cut(s) 320
AsuHPI GGTGA 6 cut(s) 44, 404, 781, 858, 1292, 1382
AsuII TTCGAA 1 cut(s) 216
AvaII GGWCC 1 cut(s) 1289
BbsI GAAGAC 1 cut(s) 719
BbvI GCAGC 1 cut(s) 872
BccI CCATC 3 cut(s) 643, 1166, 1265
BcgI CGANNNNNNTGC 2 cut(s) 1037, 1071
BciT130I CCWGG 2 cut(s) 767, 885
BciVI GTATCC 1 cut(s) 637
BclI TGATCA 1 cut(s) 1083
BcnI CCSGG 1 cut(s) 320
BcoDI GTCTC 4 cut(s) 443, 671, 976, 1048
BfuAI ACCTGC 1 cut(s) 1186
BfuI GTATCC 1 cut(s) 637
BisI GCNGC 2 cut(s) 54, 861
BlsI GCNGC 2 cut(s) 55, 862
BmcAI AGTACT 1 cut(s) 400
Bme1390I CCNGG 3 cut(s) 320, 767, 885
Bme18I GGWCC 1 cut(s) 1289
BmgBI CACGTC 1 cut(s) 1121
BmgT120I GGNCC 1 cut(s) 1289
BmrFI CCNGG 3 cut(s) 320, 767, 885
BmrI ACTGGG 1 cut(s) 525
BmsI GCATC 2 cut(s) 302, 872
BmuI ACTGGG 1 cut(s) 525
BpiI GAAGAC 1 cut(s) 719
BplI GAGNNNNNCTC 2 cut(s) 736, 768
BpmI CTGGAG 2 cut(s) 669, 867
Bpu14I TTCGAA 1 cut(s) 216
BpuMI CCSGG 1 cut(s) 320
BsaBI GATNNNNATC 1 cut(s) 699
BsaI GGTCTC 1 cut(s) 1048
BsaJI CCNNGG 2 cut(s) 51, 884
BsaWI WCCGGW 2 cut(s) 374, 781
Bsc4I CCNNNNNNNGG 1 cut(s) 541
Bse118I RCCGGY 2 cut(s) 374, 781
Bse1I ACTGG 4 cut(s) 27, 520, 652, 1243
Bse3DI GCAATG 1 cut(s) 1010
Bse8I GATNNNNATC 1 cut(s) 699
BseBI CCWGG 2 cut(s) 767, 885
BseDI CCNNGG 2 cut(s) 51, 884
BseGI GGATG 1 cut(s) 526
BseJI GATNNNNATC 1 cut(s) 699
BseLI CCNNNNNNNGG 1 cut(s) 541
BseMI GCAATG 1 cut(s) 1010
BseMII CTCAG 4 cut(s) 212, 231, 518, 974
BseNI ACTGG 4 cut(s) 27, 520, 652, 1243
BseRI GAGGAG 2 cut(s) 259, 560
BseXI GCAGC 1 cut(s) 872
Bsh1236I CGCG 1 cut(s) 53
Bsh1285I CGRYCG 1 cut(s) 51
BshFI GGCC 1 cut(s) 304
BshTI ACCGGT 2 cut(s) 374, 781
BsiEI CGRYCG 1 cut(s) 51
BsiSI CCGG 3 cut(s) 319, 375, 782
BslFI GGGAC 1 cut(s) 1348
BslI CCNNNNNNNGG 1 cut(s) 541
BsmAI GTCTC 4 cut(s) 443, 671, 976, 1048
BsmBI CGTCTC 1 cut(s) 671
BsmFI GGGAC 1 cut(s) 1348
BsmI GAATGC 1 cut(s) 295
BsnI GGCC 1 cut(s) 304
Bso31I GGTCTC 1 cut(s) 1048
Bsp119I TTCGAA 1 cut(s) 216
Bsp143I GATC 8 cut(s) 188, 360, 383, 523, 694, 1033, 1048, 1083
BspACI CCGC 3 cut(s) 51, 53, 999
BspANI GGCC 1 cut(s) 304
BspCNI CTCAG 4 cut(s) 211, 232, 519, 975
BspFNI CGCG 1 cut(s) 53
BspMI ACCTGC 1 cut(s) 1186
BspPI GGATC 4 cut(s) 196, 391, 1041, 1043
BspQI GCTCTTC 1 cut(s) 955
BspT104I TTCGAA 1 cut(s) 216
BspTNI GGTCTC 1 cut(s) 1048
BsrBI CCGCTC 1 cut(s) 1001
BsrDI GCAATG 1 cut(s) 1010
BsrFI RCCGGY 2 cut(s) 374, 781
BsrI ACTGG 4 cut(s) 27, 520, 652, 1243
BssAI RCCGGY 2 cut(s) 374, 781
BssECI CCNNGG 2 cut(s) 51, 884
BssMI GATC 8 cut(s) 188, 360, 383, 523, 694, 1033, 1048, 1083
Bst2UI CCWGG 2 cut(s) 767, 885
Bst4CI ACNGT 3 cut(s) 392, 427, 1304
Bst6I CTCTTC 4 cut(s) 552, 588, 955, 1136
BstBI TTCGAA 1 cut(s) 216
BstC8I GCNNGC 2 cut(s) 1260, 1376
BstDEI CTNAG 5 cut(s) 198, 240, 527, 909, 983
BstDSI CCRYGG 1 cut(s) 51
BstF5I GGATG 1 cut(s) 526
BstFNI CGCG 1 cut(s) 53
BstKTI GATC 8 cut(s) 191, 363, 386, 526, 697, 1036, 1051, 1086
BstMAI GTCTC 4 cut(s) 443, 671, 976, 1048
BstMBI GATC 8 cut(s) 188, 360, 383, 523, 694, 1033, 1048, 1083
BstMCI CGRYCG 1 cut(s) 51
BstNI CCWGG 2 cut(s) 767, 885
BstNSI RCATGY 1 cut(s) 456
BstSCI CCNGG 3 cut(s) 318, 765, 883
BstUI CGCG 1 cut(s) 53
BstV1I GCAGC 1 cut(s) 872
BstV2I GAAGAC 1 cut(s) 719
BstX2I RGATCY 1 cut(s) 383
BstYI RGATCY 1 cut(s) 383
BsuI GTATCC 1 cut(s) 637
BsuRI GGCC 1 cut(s) 304
BtgI CCRYGG 1 cut(s) 51
BtrI CACGTC 1 cut(s) 1121
BtsCI GGATG 1 cut(s) 526
BtsIMutI CAGTG 1 cut(s) 34
BveI ACCTGC 1 cut(s) 1186
Cac8I GCNNGC 2 cut(s) 1260, 1376
Cfr10I RCCGGY 2 cut(s) 374, 781
Cfr13I GGNCC 1 cut(s) 1289
Cfr42I CCGCGG 1 cut(s) 54
CsiI ACCWGGT 1 cut(s) 765
Csp6I GTAC 4 cut(s) 372, 399, 531, 1233
CspAI ACCGGT 2 cut(s) 374, 781
CviAII CATG 6 cut(s) 120, 262, 453, 838, 1087, 1385
CviQI GTAC 4 cut(s) 372, 399, 531, 1233
DdeI CTNAG 5 cut(s) 198, 240, 527, 909, 983
DpnI GATC 8 cut(s) 190, 362, 385, 525, 696, 1035, 1050, 1085
DpnII GATC 8 cut(s) 188, 360, 383, 523, 694, 1033, 1048, 1083
DraI TTTAAA 1 cut(s) 876
Eam1104I CTCTTC 4 cut(s) 552, 588, 955, 1136
EarI CTCTTC 4 cut(s) 552, 588, 955, 1136
Eco31I GGTCTC 1 cut(s) 1048
Eco32I GATATC 1 cut(s) 256
Eco47I GGWCC 1 cut(s) 1289
Eco57I CTGAAG 4 cut(s) 387, 459, 557, 936
EcoO109I RGGNCCY 1 cut(s) 1289
EcoRII CCWGG 2 cut(s) 765, 883
EcoRV GATATC 1 cut(s) 256
EcoT22I ATGCAT 1 cut(s) 295
Esp3I CGTCTC 1 cut(s) 671
FaeI CATG 6 cut(s) 123, 265, 456, 841, 1090, 1388
FalI AAGNNNNNCTT 2 cut(s) 402, 434
FaqI GGGAC 1 cut(s) 1348
FatI CATG 6 cut(s) 119, 261, 452, 837, 1086, 1384
FauNDI CATATG 1 cut(s) 741
FbaI TGATCA 1 cut(s) 1083
FblI GTMKAC 1 cut(s) 938
Fnu4HI GCNGC 2 cut(s) 54, 861
FokI GGATG 1 cut(s) 533
Fsp4HI GCNGC 2 cut(s) 54, 861
GluI GCNGC 2 cut(s) 54, 861
GsuI CTGGAG 2 cut(s) 669, 867
HaeIII GGCC 1 cut(s) 304
HapII CCGG 3 cut(s) 319, 375, 782
Hin1II CATG 6 cut(s) 123, 265, 456, 841, 1090, 1388
HindIII AAGCTT 1 cut(s) 416
HinfI GANTC 4 cut(s) 64, 110, 218, 350
HpaII CCGG 3 cut(s) 319, 375, 782
HphI GGTGA 6 cut(s) 44, 404, 781, 858, 1292, 1382
Hpy166II GTNNAC 2 cut(s) 939, 1390
Hpy188III TCNNGA 3 cut(s) 68, 470, 709
Hpy8I GTNNAC 2 cut(s) 939, 1390
HpyAV CCTTC 5 cut(s) 199, 604, 667, 955, 1345
HpyCH4III ACNGT 3 cut(s) 392, 427, 1304
HpyCH4IV ACGT 2 cut(s) 1120, 1356
HpyCH4V TGCA 6 cut(s) 76, 179, 293, 1124, 1205, 1262
HpyF3I CTNAG 5 cut(s) 198, 240, 527, 909, 983
HpySE526I ACGT 2 cut(s) 1120, 1356
Hsp92II CATG 6 cut(s) 123, 265, 456, 841, 1090, 1388
Ksp22I TGATCA 1 cut(s) 1083
KspI CCGCGG 1 cut(s) 54
Kzo9I GATC 8 cut(s) 188, 360, 383, 523, 694, 1033, 1048, 1083
LguI GCTCTTC 1 cut(s) 955
LmnI GCTCC 3 cut(s) 265, 1006, 1192
Lsp1109I GCAGC 1 cut(s) 872
LweI GCATC 2 cut(s) 302, 872
MabI ACCWGGT 1 cut(s) 765
MaeII ACGT 2 cut(s) 1120, 1356
MaeIII GTNAC 6 cut(s) 338, 421, 433, 455, 769, 784
MalI GATC 8 cut(s) 190, 362, 385, 525, 696, 1035, 1050, 1085
MbiI CCGCTC 1 cut(s) 1001
MboI GATC 8 cut(s) 188, 360, 383, 523, 694, 1033, 1048, 1083
MflI RGATCY 1 cut(s) 383
MlyI GAGTC 1 cut(s) 359
MmeI TCCRAC 2 cut(s) 522, 1088
MnlI CCTC 9 cut(s) 237, 279, 477, 538, 568, 589, 760, 891, 1218
Mph1103I ATGCAT 1 cut(s) 295
MroXI GAANNNNTTC 2 cut(s) 630, 1145
MseI TTAA 2 cut(s) 875, 1128
MslI CAYNNNNRTG 1 cut(s) 118
MspA1I CMGCKG 1 cut(s) 53
MspI CCGG 3 cut(s) 319, 375, 782
MspR9I CCNGG 3 cut(s) 320, 767, 885
Mva1269I GAATGC 1 cut(s) 295
MvaI CCWGG 2 cut(s) 767, 885
MvnI CGCG 1 cut(s) 53
NciI CCSGG 1 cut(s) 320
NdeI CATATG 1 cut(s) 741
NdeII GATC 8 cut(s) 188, 360, 383, 523, 694, 1033, 1048, 1083
NlaIII CATG 6 cut(s) 123, 265, 456, 841, 1090, 1388
NmeAIII GCCGAG 1 cut(s) 1366
NmuCI GTSAC 3 cut(s) 338, 421, 769
NsiI ATGCAT 1 cut(s) 295
NspI RCATGY 1 cut(s) 456
NspV TTCGAA 1 cut(s) 216
PciI ACATGT 1 cut(s) 452
PciSI GCTCTTC 1 cut(s) 955
PcsI WCGNNNNNNNCGW 1 cut(s) 1338
PctI GAATGC 1 cut(s) 295
PdmI GAANNNNTTC 2 cut(s) 630, 1145
PfeI GAWTC 3 cut(s) 64, 110, 218
PinAI ACCGGT 2 cut(s) 374, 781
PkrI GCNGC 2 cut(s) 55, 862
PleI GAGTC 1 cut(s) 358
PpsI GAGTC 1 cut(s) 358
PpuMI RGGWCCY 1 cut(s) 1289
PscI ACATGT 1 cut(s) 452
Psp5II RGGWCCY 1 cut(s) 1289
Psp6I CCWGG 2 cut(s) 765, 883
PspGI CCWGG 2 cut(s) 765, 883
PspPI GGNCC 1 cut(s) 1289
PspPPI RGGWCCY 1 cut(s) 1289
PsuI RGATCY 1 cut(s) 383
RsaI GTAC 4 cut(s) 373, 400, 532, 1234
RsaNI GTAC 4 cut(s) 372, 399, 531, 1233
RseI CAYNNNNRTG 1 cut(s) 118
SacII CCGCGG 1 cut(s) 54
SapI GCTCTTC 1 cut(s) 955
SaqAI TTAA 2 cut(s) 875, 1128
SatI GCNGC 2 cut(s) 54, 861
Sau3AI GATC 8 cut(s) 188, 360, 383, 523, 694, 1033, 1048, 1083
Sau96I GGNCC 1 cut(s) 1289
ScaI AGTACT 1 cut(s) 400
SchI GAGTC 1 cut(s) 359
ScrFI CCNGG 3 cut(s) 320, 767, 885
SexAI ACCWGGT 1 cut(s) 765
SfaNI GCATC 2 cut(s) 302, 872
Sfr303I CCGCGG 1 cut(s) 54
SfuI TTCGAA 1 cut(s) 216
SgrBI CCGCGG 1 cut(s) 54
SinI GGWCC 1 cut(s) 1289
SmiMI CAYNNNNRTG 1 cut(s) 118
SsiI CCGC 3 cut(s) 51, 53, 999
SspI AATATT 1 cut(s) 310
StyD4I CCNGG 3 cut(s) 318, 765, 883
TaaI ACNGT 3 cut(s) 392, 427, 1304
TaiI ACGT 2 cut(s) 1123, 1359
TaqI TCGA 5 cut(s) 62, 216, 363, 499, 1149
TaqII GACCGA 1 cut(s) 1030
TatI WGTACW 1 cut(s) 398
TauI GCSGC 1 cut(s) 56
TfiI GAWTC 3 cut(s) 64, 110, 218
Tru1I TTAA 2 cut(s) 875, 1128
Tru9I TTAA 2 cut(s) 875, 1128
TscAI CASTG 1 cut(s) 34
TseFI GTSAC 3 cut(s) 338, 421, 769
TseI GCWGC 1 cut(s) 860
Tsp45I GTSAC 3 cut(s) 338, 421, 769
TspDTI ATGAA 7 cut(s) 48, 108, 123, 623, 686, 1172, 1299
TspGWI ACGGA 1 cut(s) 1292
TspRI CASTG 1 cut(s) 34
VpaK11BI GGWCC 1 cut(s) 1289
XceI RCATGY 1 cut(s) 456
XmiI GTMKAC 1 cut(s) 938
XmnI GAANNNNTTC 2 cut(s) 630, 1145
ZrmI AGTACT 1 cut(s) 400
Zsp2I ATGCAT 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.