Rh4AG417100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0003792
Physical Location & Seq
Reverse (-)
71498660 .. 71501234
2575 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG417100.1

Sequence Viewer

Length: 1626 bp
ATGGGTGAATCAATAGTGGGTTTTCCAAAAGATGCTGATAAGATGGGAGAGATAGCTGGTACAGATTCATCAAATCCTTCTCTGGAAGTGTCGGTTTCATTTGGTAGGTTTGAGAATGATTCATTATCTTGGGAGAAATGGTCGGCATTTTCGCCAAATAAGTACTTGGAAGAAGTTGAGAAGTGTGCTACTCCTGGTTCGGTAGCTCAGAAAAGGGCTTATTTTGAAGCTCATTACAAGAGGATAGCTGCTCGAAAAGCCGAGGAGCTTCTGGAACAAGAGAAGCAAATGCAAGATGATGATCCCTTAAAGTCAGATGATCAAAATAATGGAGATCAGATCTGTTGTGGTACGGATTATGGAATTGATGTAGCTACCAGTCAAACTAGTGCTCAAGCAAATAGTCAAGAGCCCAATATAGAGAATGGGATGAGCAGTACTCATGTGGAAGATCTCAAGGAGGAGGATGATGAGGATGTTTTCACTATGGAATGTCAAACATCATCGATTGAGGGTAGAGAAATAGAGGAAACTGATAGCGGAGTGGTTAGTCCGAAGACTCCAAAGCTGAACAGACCAGAAGAACTTGTTTTGGTAAAAGAAGTGGAAACCATCACTGCTGACACTCAAGAAACAATACAGCAAGTTCCAAAGACTTTGGACAGTGATACAGGCAATGCTCCAGAAGTTAAAGAGGAAAAAGCGAGATTCGATCTTCAGAAGAGATCTCAGAAGGTTACTCCAGTGAGCAAGGAGAGGACAGTGGCAAAGGCCAAGAAGAAATCAATTTCACCAATGACTAAAACACCTCAGAATCCGACCCCTAGAGTGTCAAAGTTACCGCAGAATTCCACCCCTAGAGTGTCAAAGATACCGCAGAATACTACCCCTAGAGTGTCAAAGATACTGCAGAATACCACCCCTAGTGTGTCAAAGCCAATATCAGCTCCTACCGGAGCAAAATCTGCACCACGATCCTCAGTAACGAAGGCCAATGGTTCATCTTTAACTAGGAGCACAAATCAGTCAAGTGAAAAAACCAAGAAAGTGCCTCCTAAATCTCTGCACATGTCACTCAGTTTCAATCCTAAAAAGTCTGATTCAGCTTCAGAAACTGTGGTTACAGCTAGAAAATCTTTGATTATGGAGCAAATGGGTGATAAGGACATTGTCAAACGAGCATTCAAGACATTTCAAAACAGTGTCAACCAATTGAAATCCTCTAATGAAGAGAAACCTTCAGCACCAATGCAGCTCCCTACAAAGTCGAAAGAACCAAAGGTTTCCACTCCTATGCCTCTGCCGAAAGACACTGGAGGGTCACTCAAAACGAGTTATCCGGATAAAAGAAATGCCAAGGCTGCTCCATCATCTTTTGGCCTGAGAAGTGAAGAAAGAGCAGAGAAACGGAAGGAGTTAACAAACAACTTGGCGAAACCCAATGCCAGAGATGCAGAGAGAGCACACTTCCAGCCAAAGTCAAAGGAGCAAAAGGAGGCAGAGATAAAAAAGCTAAGACAGAGTCTTAAGTTCAAGGCTACACCAACGCCTGATTCTTATCGGGGACAAAAGTTATTGAAAAGTACTCTAGAAAAGGAGGGTCCTAAGAAAGAAACGCTTCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

541

Amino Acids

59.81

Weight (kDa)

9.14

Isoelectric Point (pI)

47.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 459 - 523 2.2e-13 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 1339
AciI CCGC 3 cut(s) 540, 842, 875
AclWI GGATC 2 cut(s) 296, 969
AcsI RAATTY 1 cut(s) 847
AcuI CTGAAG 3 cut(s) 701, 1092, 1224
AfaI GTAC 5 cut(s) 61, 164, 352, 439, 1585
AflII CTTAAG 1 cut(s) 1526
AflIII ACRYGT 1 cut(s) 1068
AgsI TTSAA 7 cut(s) 227, 1084, 1186, 1196, 1216, 1534, 1579
AhlI ACTAGT 1 cut(s) 386
AjnI CCWGG 1 cut(s) 193
AjuI GAANNNNNNNTTGG 2 cut(s) 1537, 1569
Alw21I GWGCWC 3 cut(s) 394, 1019, 1465
AlwI GGATC 2 cut(s) 296, 969
AlwNI CAGNNNCTG 1 cut(s) 1115
Aor13HI TCCGGA 1 cut(s) 1339
AoxI GGCC 3 cut(s) 771, 990, 1378
ApeKI GCWGC 3 cut(s) 248, 1252, 1361
ApoI RAATTY 1 cut(s) 847
Asp700I GAANNNNTTC 1 cut(s) 1617
AspS9I GGNCC 1 cut(s) 1601
AsuHPI GGTGA 3 cut(s) 17, 783, 1169
AvaII GGWCC 1 cut(s) 1601
BanII GRGCYC 1 cut(s) 414
BbsI GAAGAC 1 cut(s) 563
Bbv12I GWGCWC 3 cut(s) 394, 1019, 1465
BbvI GCAGC 3 cut(s) 235, 1264, 1348
BccI CCATC 3 cut(s) 37, 620, 1375
BciT130I CCWGG 1 cut(s) 195
BclI TGATCA 1 cut(s) 319
BcuI ACTAGT 1 cut(s) 386
BfaI CTAG 8 cut(s) 387, 825, 858, 891, 924, 1011, 1128, 1589
BfmI CTRYAG 1 cut(s) 908
BfrI CTTAAG 1 cut(s) 1526
BglII AGATCT 3 cut(s) 339, 451, 725
BisI GCNGC 3 cut(s) 249, 1253, 1362
BlsI GCNGC 3 cut(s) 250, 1254, 1363
BmcAI AGTACT 3 cut(s) 164, 439, 1585
Bme1390I CCNGG 1 cut(s) 195
Bme18I GGWCC 1 cut(s) 1601
BmgT120I GGNCC 1 cut(s) 1601
BmiI GGNNCC 1 cut(s) 1602
BmrFI CCNGG 1 cut(s) 195
BmsI GCATC 2 cut(s) 22, 1441
BpiI GAAGAC 1 cut(s) 563
BplI GAGNNNNNCTC 4 cut(s) 424, 456, 1308, 1340
BpmI CTGGAG 3 cut(s) 666, 726, 1335
BpuEI CTTGAG 3 cut(s) 378, 440, 612
Bsa29I ATCGAT 1 cut(s) 506
BsaBI GATNNNNATC 2 cut(s) 300, 1557
BsaJI CCNNGG 2 cut(s) 261, 1356
BsaWI WCCGGW 2 cut(s) 953, 1339
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bse1I ACTGG 3 cut(s) 378, 743, 1318
Bse3DI GCAATG 1 cut(s) 682
Bse8I GATNNNNATC 2 cut(s) 300, 1557
BseAI TCCGGA 1 cut(s) 1339
BseBI CCWGG 1 cut(s) 195
BseCI ATCGAT 1 cut(s) 506
BseDI CCNNGG 2 cut(s) 261, 1356
BseGI GGATG 3 cut(s) 435, 472, 481
BseJI GATNNNNATC 2 cut(s) 300, 1557
BseMI GCAATG 1 cut(s) 682
BseMII CTCAG 6 cut(s) 221, 743, 824, 993, 1090, 1373
BseNI ACTGG 3 cut(s) 378, 743, 1318
BseRI GAGGAG 2 cut(s) 278, 476
BseXI GCAGC 3 cut(s) 235, 1264, 1348
BsgI GTGCAG 2 cut(s) 951, 1049
BshFI GGCC 3 cut(s) 773, 992, 1380
BshVI ATCGAT 1 cut(s) 506
BsiHKAI GWGCWC 3 cut(s) 394, 1019, 1465
BsiSI CCGG 2 cut(s) 954, 1340
BslFI GGGAC 1 cut(s) 1578
BsmFI GGGAC 1 cut(s) 1578
BsmI GAATGC 1 cut(s) 1181
BsnI GGCC 3 cut(s) 773, 992, 1380
Bsp1286I GDGCHC 4 cut(s) 394, 414, 1019, 1465
Bsp13I TCCGGA 1 cut(s) 1339
Bsp143I GATC 8 cut(s) 301, 319, 334, 339, 451, 712, 725, 974
BspACI CCGC 3 cut(s) 540, 842, 875
BspANI GGCC 3 cut(s) 773, 992, 1380
BspCNI CTCAG 6 cut(s) 220, 742, 823, 992, 1089, 1374
BspDI ATCGAT 1 cut(s) 506
BspEI TCCGGA 1 cut(s) 1339
BspLI GGNNCC 1 cut(s) 1602
BspMAI CTGCAG 1 cut(s) 912
BspPI GGATC 2 cut(s) 296, 969
BspTI CTTAAG 1 cut(s) 1526
BsrDI GCAATG 1 cut(s) 682
BsrI ACTGG 3 cut(s) 378, 743, 1318
BssECI CCNNGG 2 cut(s) 261, 1356
BssMI GATC 8 cut(s) 301, 319, 334, 339, 451, 712, 725, 974
BssT1I CCWWGG 1 cut(s) 1356
Bst2UI CCWGG 1 cut(s) 195
Bst4CI ACNGT 4 cut(s) 665, 763, 1117, 1202
Bst6I CTCTTC 2 cut(s) 716, 1224
BstAFI CTTAAG 1 cut(s) 1526
BstAPI GCANNNNNTGC 1 cut(s) 965
BstDEI CTNAG 8 cut(s) 207, 729, 810, 979, 1076, 1382, 1514, 1605
BstF5I GGATG 3 cut(s) 435, 472, 481
BstKTI GATC 8 cut(s) 304, 322, 337, 342, 454, 715, 728, 977
BstMBI GATC 8 cut(s) 301, 319, 334, 339, 451, 712, 725, 974
BstMWI GCNNNNNNNGC 5 cut(s) 257, 965, 1361, 1451, 1460
BstNI CCWGG 1 cut(s) 195
BstNSI RCATGY 1 cut(s) 1072
BstSCI CCNGG 1 cut(s) 193
BstSFI CTRYAG 1 cut(s) 908
BstV1I GCAGC 3 cut(s) 235, 1264, 1348
BstV2I GAAGAC 1 cut(s) 563
BstX2I RGATCY 3 cut(s) 339, 451, 725
BstYI RGATCY 3 cut(s) 339, 451, 725
Bsu15I ATCGAT 1 cut(s) 506
BsuRI GGCC 3 cut(s) 773, 992, 1380
BsuTUI ATCGAT 1 cut(s) 506
BtsCI GGATG 3 cut(s) 435, 472, 481
BtsI GCAGTG 1 cut(s) 615
BtsIMutI CAGTG 6 cut(s) 615, 670, 750, 768, 1207, 1311
CaiI CAGNNNCTG 1 cut(s) 1115
Cfr13I GGNCC 1 cut(s) 1601
ClaI ATCGAT 1 cut(s) 506
Csp6I GTAC 5 cut(s) 60, 163, 351, 438, 1584
CviAII CATG 2 cut(s) 443, 1069
CviQI GTAC 5 cut(s) 60, 163, 351, 438, 1584
DdeI CTNAG 8 cut(s) 207, 729, 810, 979, 1076, 1382, 1514, 1605
DpnI GATC 8 cut(s) 303, 321, 336, 341, 453, 714, 727, 976
DpnII GATC 8 cut(s) 301, 319, 334, 339, 451, 712, 725, 974
Eam1104I CTCTTC 2 cut(s) 716, 1224
EarI CTCTTC 2 cut(s) 716, 1224
Eco130I CCWWGG 1 cut(s) 1356
Eco24I GRGCYC 1 cut(s) 414
Eco47I GGWCC 1 cut(s) 1601
Eco57I CTGAAG 3 cut(s) 701, 1092, 1224
EcoO109I RGGNCCY 1 cut(s) 1601
EcoRI GAATTC 1 cut(s) 847
EcoRII CCWGG 1 cut(s) 193
EcoT14I CCWWGG 1 cut(s) 1356
EcoT38I GRGCYC 1 cut(s) 414
ErhI CCWWGG 1 cut(s) 1356
FaeI CATG 2 cut(s) 446, 1072
FaiI YATR 7 cut(s) 360, 419, 444, 488, 1070, 1145, 1295
FalI AAGNNNNNCTT 1 cut(s) 1602
FaqI GGGAC 1 cut(s) 1578
FatI CATG 2 cut(s) 442, 1068
FbaI TGATCA 1 cut(s) 319
Fnu4HI GCNGC 3 cut(s) 249, 1253, 1362
FokI GGATG 3 cut(s) 442, 479, 488
FriOI GRGCYC 1 cut(s) 414
Fsp4HI GCNGC 3 cut(s) 249, 1253, 1362
FspBI CTAG 8 cut(s) 387, 825, 858, 891, 924, 1011, 1128, 1589
GluI GCNGC 3 cut(s) 249, 1253, 1362
GsuI CTGGAG 3 cut(s) 666, 726, 1335
HaeIII GGCC 3 cut(s) 773, 992, 1380
HapII CCGG 2 cut(s) 954, 1340
Hin1II CATG 2 cut(s) 446, 1072
HincII GTYRAC 2 cut(s) 1207, 1419
HindII GTYRAC 2 cut(s) 1207, 1419
HinfI GANTC 9 cut(s) 8, 65, 119, 559, 708, 814, 1100, 1522, 1553
HpaI GTTAAC 1 cut(s) 1419
HpaII CCGG 2 cut(s) 954, 1340
HphI GGTGA 3 cut(s) 17, 783, 1169
Hpy166II GTNNAC 2 cut(s) 1207, 1419
Hpy188III TCNNGA 8 cut(s) 83, 272, 407, 629, 683, 1186, 1340, 1589
Hpy8I GTNNAC 2 cut(s) 1207, 1419
HpyAV CCTTC 5 cut(s) 87, 727, 982, 1248, 1405
HpyCH4III ACNGT 4 cut(s) 665, 763, 1117, 1202
HpyCH4V TGCA 6 cut(s) 292, 910, 968, 1066, 1252, 1454
HpyF10VI GCNNNNNNNGC 5 cut(s) 257, 965, 1361, 1451, 1460
HpyF3I CTNAG 8 cut(s) 207, 729, 810, 979, 1076, 1382, 1514, 1605
Hsp92II CATG 2 cut(s) 446, 1072
Kpn2I TCCGGA 1 cut(s) 1339
Ksp22I TGATCA 1 cut(s) 319
KspAI GTTAAC 1 cut(s) 1419
Kzo9I GATC 8 cut(s) 301, 319, 334, 339, 451, 712, 725, 974
LmnI GCTCC 9 cut(s) 265, 685, 952, 956, 1014, 1147, 1260, 1369, 1486
Lsp1109I GCAGC 3 cut(s) 235, 1264, 1348
LweI GCATC 2 cut(s) 22, 1441
MaeI CTAG 8 cut(s) 387, 825, 858, 891, 924, 1011, 1128, 1589
MaeIII GTNAC 6 cut(s) 736, 837, 982, 1071, 1120, 1320
MalI GATC 8 cut(s) 303, 321, 336, 341, 453, 714, 727, 976
MboI GATC 8 cut(s) 301, 319, 334, 339, 451, 712, 725, 974
MboII GAAGA 9 cut(s) 182, 461, 568, 593, 707, 733, 790, 1241, 1403
MfeI CAATTG 1 cut(s) 1211
MflI RGATCY 3 cut(s) 339, 451, 725
MhlI GDGCHC 4 cut(s) 394, 414, 1019, 1465
MluCI AATT 4 cut(s) 363, 786, 847, 1211
MlyI GAGTC 2 cut(s) 553, 1531
MmeI TCCRAC 1 cut(s) 842
MroI TCCGGA 1 cut(s) 1339
MroXI GAANNNNTTC 1 cut(s) 1617
MseI TTAA 5 cut(s) 308, 690, 1007, 1418, 1527
MslI CAYNNNNRTG 1 cut(s) 1292
MspCI CTTAAG 1 cut(s) 1526
MspI CCGG 2 cut(s) 954, 1340
MspR9I CCNGG 1 cut(s) 195
MunI CAATTG 1 cut(s) 1211
Mva1269I GAATGC 1 cut(s) 1181
MvaI CCWGG 1 cut(s) 195
MwoI GCNNNNNNNGC 5 cut(s) 257, 965, 1361, 1451, 1460
NdeII GATC 8 cut(s) 301, 319, 334, 339, 451, 712, 725, 974
NlaIII CATG 2 cut(s) 446, 1072
NlaIV GGNNCC 1 cut(s) 1602
NmeAIII GCCGAG 1 cut(s) 286
NmuCI GTSAC 2 cut(s) 1071, 1320
NspI RCATGY 1 cut(s) 1072
PciI ACATGT 1 cut(s) 1068
PctI GAATGC 1 cut(s) 1181
PdmI GAANNNNTTC 1 cut(s) 1617
PfeI GAWTC 7 cut(s) 8, 65, 119, 708, 814, 1100, 1553
PflFI GACNNNGTC 2 cut(s) 1169, 1521
PkrI GCNGC 3 cut(s) 250, 1254, 1363
PleI GAGTC 2 cut(s) 553, 1530
PpsI GAGTC 2 cut(s) 553, 1530
PpuMI RGGWCCY 1 cut(s) 1601
PscI ACATGT 1 cut(s) 1068
Psp5II RGGWCCY 1 cut(s) 1601
Psp6I CCWGG 1 cut(s) 193
PspGI CCWGG 1 cut(s) 193
PspN4I GGNNCC 1 cut(s) 1602
PspPI GGNCC 1 cut(s) 1601
PspPPI RGGWCCY 1 cut(s) 1601
PstI CTGCAG 1 cut(s) 912
PstNI CAGNNNCTG 1 cut(s) 1115
PsuI RGATCY 3 cut(s) 339, 451, 725
PsyI GACNNNGTC 2 cut(s) 1169, 1521
RsaI GTAC 5 cut(s) 61, 164, 352, 439, 1585
RsaNI GTAC 5 cut(s) 60, 163, 351, 438, 1584
RseI CAYNNNNRTG 1 cut(s) 1292
SaqAI TTAA 5 cut(s) 308, 690, 1007, 1418, 1527
SatI GCNGC 3 cut(s) 249, 1253, 1362
Sau3AI GATC 8 cut(s) 301, 319, 334, 339, 451, 712, 725, 974
Sau96I GGNCC 1 cut(s) 1601
ScaI AGTACT 3 cut(s) 164, 439, 1585
SchI GAGTC 2 cut(s) 553, 1531
ScrFI CCNGG 1 cut(s) 195
SduI GDGCHC 4 cut(s) 394, 414, 1019, 1465
SfaNI GCATC 2 cut(s) 22, 1441
SfcI CTRYAG 1 cut(s) 908
SinI GGWCC 1 cut(s) 1601
SmiMI CAYNNNNRTG 1 cut(s) 1292
SmlI CTYRAG 4 cut(s) 393, 455, 627, 1526
SmoI CTYRAG 4 cut(s) 393, 455, 627, 1526
SpeI ACTAGT 1 cut(s) 386
Sse9I AATT 4 cut(s) 363, 786, 847, 1211
SsiI CCGC 3 cut(s) 540, 842, 875
SspMI CTAG 8 cut(s) 387, 825, 858, 891, 924, 1011, 1128, 1589
StyD4I CCNGG 1 cut(s) 193
StyI CCWWGG 1 cut(s) 1356
TaaI ACNGT 4 cut(s) 665, 763, 1117, 1202
TaqI TCGA 4 cut(s) 253, 506, 711, 1268
TasI AATT 4 cut(s) 363, 786, 847, 1211
TatI WGTACW 3 cut(s) 162, 437, 1583
TfiI GAWTC 7 cut(s) 8, 65, 119, 708, 814, 1100, 1553
Tru1I TTAA 5 cut(s) 308, 690, 1007, 1418, 1527
Tru9I TTAA 5 cut(s) 308, 690, 1007, 1418, 1527
TscAI CASTG 6 cut(s) 622, 670, 750, 768, 1207, 1318
TseFI GTSAC 2 cut(s) 1071, 1320
TseI GCWGC 3 cut(s) 248, 1252, 1361
Tsp45I GTSAC 2 cut(s) 1071, 1320
TspDTI ATGAA 5 cut(s) 57, 87, 111, 990, 1242
TspGWI ACGGA 2 cut(s) 368, 1423
TspRI CASTG 6 cut(s) 622, 670, 750, 768, 1207, 1318
Tth111I GACNNNGTC 2 cut(s) 1169, 1521
Vha464I CTTAAG 1 cut(s) 1526
VpaK11BI GGWCC 1 cut(s) 1601
XapI RAATTY 1 cut(s) 847
XbaI TCTAGA 1 cut(s) 1588
XceI RCATGY 1 cut(s) 1072
XmnI GAANNNNTTC 1 cut(s) 1617
XspI CTAG 8 cut(s) 387, 825, 858, 891, 924, 1011, 1128, 1589
ZrmI AGTACT 3 cut(s) 164, 439, 1585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.