RLG00000005795

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0003792
Physical Location & Seq
Forward (+)
1835215 .. 1838440
3226 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005795

Sequence Viewer

Length: 1692 bp
ATGCCTCCTCTGATCAGTGCCTTCAACTTCTTTAGGTCTTTCTGTGAAGCACTGAATGAGAAATCTACAGTGCGTGATCCAATAGTGGGTTTTCCAAAAGATGCTGATAAGATGGGAGAGATAGCTGGTACAGATTCATCAAATCCTTCTCTGGAAGTGTCGGTTTCGTTTGGTAGGTTTGAGAATGATTCATTATCTTGGGAGAAATGGTCGGCATTTTCGCCAAATAAGTACTTGGAAGAAGTCGAGAAGTGTGCTACTCCTGGTTCGGTAGCTCAGAAAAGGGCTTATTTTGAAGCTCATTACAAGAGGATAGCTGCTCGAAAAGCCGAGGAGCTTCTGGAACAAGAGAAGCAAATGCAAGATGATGATCCCTTAAAGTCAGATGATAAAAATAATGGAGATCAGATCTGTTGTGGTACGGATTATGGAATTGATACAGCTACCAGTCAAACTAGTGCTCAAGCAAATAGTCAAGAGCCCAATATAGAGAATGGGATGAGCAGTACTCATGTGGAAGATCTCAAGGAGGATGATGAGGATGTTTTCACTATGGAATGTCAAACATCATTGATTGAGGGTAGAGAAATAGAGGAAACTGATAGCGGAGTGGTTAGTCCGAAGACTCCAAAGCTGAACAGACCAGAAGAACTTGTTTTGGTAAAAGAAGGGGAAACCATTACAGCTGACACTCAAGAAACAATACAGGAAGTTCCAAAGACTTTGGACAGTGATACAGGCAATGCTCCAGAATTTAAAGAGGAAAAGGCGAGATTCGATCTTCAGAAGAGATCTCAGCAGGTTACTCCAGTGAGCAAGGAGAGGACAGTGGCAAAGGCCAAGAAGAAAACAATTTCACCAATGACTAAAACACCTCAGAATCCGACCCCTAGAGTGTCAAAGTTACCACAGAATTCCACCCCTAGAGTGTCAAAGATACCGCAGAATACCACCCCTAGAGTGTCAAAGATACTGCAGAATACCACCCCTAGAGTGTCAAAGCCAATATCAGCTTCTACCGGAGCAAAATCTGCACCAAGATCCTCAGTGACGAAGGCCAATGGTTCATCTTTAACTAGGAGCACAAATCAGTCAAGTGAAAAAACCAAGAAAGTTCCTCCTAAATCTCTGCACATGTCACTCAGTTTCAATCCTAAAAAGTCTGATTTAGCTACAGAAACTGTGGTTACAGCTAGAAAATCTTTGATTATGGAGCAAATGGGTGATAAGGACATTGTCAAACGAGCATTCAAGACATTTCAAAACAGTGTCAACCAATTGAAATCCTCTAATGAAGAGAAACCTTCAGCACCAATGCAGCTCCCTACAAAGTCGAAAGAACCAAAGGTTTCCACTCCTATGCCTCTGCCGAAAGACAATGGAGGGTCACTCAAAACGAGTTATCAGGATAAAAGAAATGCCAAGGCTGCTCCATCATCTTTTGGCCCGAGAAGTGAAGAAAGAGCAGAGAAACGGAAGGAGTTAACAAACAAATTGACAAAACCCAATGCCAGAGATGCAGAGAGTGCACACTTCCACCCAAAGTCAAAGGAGCAAAAGGAGGCAGAAATAAAAAAGCTAAGACAGAGTCTTAAGTTCAAGGCTAAACCAACGCCTGATTCTTATCGGGGACAAAAGTTATCGAAAAGTACTCTAGAAAAGGATGGTCCTAAGAAAGAAACGCGTCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

564

Amino Acids

62.56

Weight (kDa)

9.27

Isoelectric Point (pI)

45.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 484 - 550 4.4e-11 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 790
AccII CGCG 1 cut(s) 1684
AciI CCGC 2 cut(s) 606, 941
AclWI GGATC 3 cut(s) 71, 365, 1035
AcsI RAATTY 2 cut(s) 752, 913
AcuI CTGAAG 2 cut(s) 767, 1290
AfaI GTAC 5 cut(s) 130, 233, 421, 508, 1651
AfiI CCNNNNNNNGG 1 cut(s) 86
AflII CTTAAG 1 cut(s) 1592
AflIII ACRYGT 2 cut(s) 1134, 1682
AgsI TTSAA 7 cut(s) 25, 296, 1150, 1252, 1262, 1282, 1600
AhlI ACTAGT 1 cut(s) 455
AjnI CCWGG 1 cut(s) 262
AjuI GAANNNNNNNTTGG 4 cut(s) 997, 1029, 1603, 1635
Alw21I GWGCWC 3 cut(s) 463, 1085, 1531
Alw44I GTGCAC 1 cut(s) 1527
AlwI GGATC 3 cut(s) 71, 365, 1035
AlwNI CAGNNNCTG 1 cut(s) 1181
Ama87I CYCGRG 1 cut(s) 1447
AoxI GGCC 3 cut(s) 837, 1056, 1444
ApaLI GTGCAC 1 cut(s) 1527
ApeKI GCWGC 3 cut(s) 317, 1318, 1427
ApoI RAATTY 2 cut(s) 752, 913
AspS9I GGNCC 2 cut(s) 1445, 1667
AsuHPI GGTGA 2 cut(s) 849, 1235
AvaI CYCGRG 1 cut(s) 1447
AvaII GGWCC 1 cut(s) 1667
BaeGI GKGCMC 1 cut(s) 1531
BanII GRGCYC 1 cut(s) 483
BbsI GAAGAC 1 cut(s) 629
Bbv12I GWGCWC 3 cut(s) 463, 1085, 1531
BbvI GCAGC 3 cut(s) 304, 1330, 1414
BccI CCATC 3 cut(s) 106, 1441, 1658
BcgI CGANNNNNNTGC 2 cut(s) 236, 270
BciT130I CCWGG 1 cut(s) 264
BclI TGATCA 1 cut(s) 12
BcuI ACTAGT 1 cut(s) 455
BfaI CTAG 8 cut(s) 456, 891, 924, 957, 990, 1077, 1194, 1655
BfmI CTRYAG 3 cut(s) 66, 974, 1173
BfrI CTTAAG 1 cut(s) 1592
BfuAI ACCTGC 1 cut(s) 790
BglII AGATCT 3 cut(s) 408, 520, 791
BisI GCNGC 3 cut(s) 318, 1319, 1428
BlsI GCNGC 3 cut(s) 319, 1320, 1429
BmcAI AGTACT 3 cut(s) 233, 508, 1651
Bme1390I CCNGG 1 cut(s) 264
Bme18I GGWCC 1 cut(s) 1667
BmeT110I CYCGRG 1 cut(s) 1447
BmgT120I GGNCC 2 cut(s) 1445, 1667
BmrFI CCNGG 1 cut(s) 264
BmsI GCATC 2 cut(s) 91, 1507
BpiI GAAGAC 1 cut(s) 629
BplI GAGNNNNNCTC 4 cut(s) 493, 525, 1374, 1406
BpmI CTGGAG 2 cut(s) 732, 792
BpuEI CTTGAG 3 cut(s) 447, 509, 678
BsaBI GATNNNNATC 2 cut(s) 369, 1623
BsaJI CCNNGG 2 cut(s) 330, 1422
BsaWI WCCGGW 1 cut(s) 1019
BsaXI ACNNNNNCTCC 2 cut(s) 194, 224
Bsc4I CCNNNNNNNGG 1 cut(s) 86
Bse1I ACTGG 2 cut(s) 447, 809
Bse3DI GCAATG 1 cut(s) 748
Bse8I GATNNNNATC 2 cut(s) 369, 1623
BseBI CCWGG 1 cut(s) 264
BseDI CCNNGG 2 cut(s) 330, 1422
BseGI GGATG 4 cut(s) 504, 538, 547, 1669
BseJI GATNNNNATC 2 cut(s) 369, 1623
BseLI CCNNNNNNNGG 1 cut(s) 86
BseMI GCAATG 1 cut(s) 748
BseMII CTCAG 5 cut(s) 290, 809, 890, 1059, 1156
BseNI ACTGG 2 cut(s) 447, 809
BseRI GAGGAG 1 cut(s) 347
BseSI GKGCMC 1 cut(s) 1531
BseXI GCAGC 3 cut(s) 304, 1330, 1414
BsgI GTGCAG 2 cut(s) 1017, 1115
Bsh1236I CGCG 1 cut(s) 1684
BshFI GGCC 3 cut(s) 839, 1058, 1446
BsiHKAI GWGCWC 3 cut(s) 463, 1085, 1531
BsiHKCI CYCGRG 1 cut(s) 1447
BsiSI CCGG 1 cut(s) 1020
BslFI GGGAC 1 cut(s) 1644
BslI CCNNNNNNNGG 1 cut(s) 86
BsmFI GGGAC 1 cut(s) 1644
BsmI GAATGC 1 cut(s) 1247
BsnI GGCC 3 cut(s) 839, 1058, 1446
BsoBI CYCGRG 1 cut(s) 1447
Bsp1286I GDGCHC 4 cut(s) 463, 483, 1085, 1531
Bsp143I GATC 9 cut(s) 12, 76, 370, 403, 408, 520, 778, 791, 1040
BspACI CCGC 2 cut(s) 606, 941
BspANI GGCC 3 cut(s) 839, 1058, 1446
BspCNI CTCAG 5 cut(s) 289, 808, 889, 1058, 1155
BspFNI CGCG 1 cut(s) 1684
BspMAI CTGCAG 1 cut(s) 978
BspMI ACCTGC 1 cut(s) 790
BspPI GGATC 3 cut(s) 71, 365, 1035
BspTI CTTAAG 1 cut(s) 1592
BsrDI GCAATG 1 cut(s) 748
BsrI ACTGG 2 cut(s) 447, 809
BssECI CCNNGG 2 cut(s) 330, 1422
BssMI GATC 9 cut(s) 12, 76, 370, 403, 408, 520, 778, 791, 1040
BssT1I CCWWGG 1 cut(s) 1422
Bst2UI CCWGG 1 cut(s) 264
Bst4CI ACNGT 5 cut(s) 70, 731, 829, 1183, 1268
Bst6I CTCTTC 2 cut(s) 782, 1290
BstAFI CTTAAG 1 cut(s) 1592
BstAPI GCANNNNNTGC 2 cut(s) 1031, 1526
BstDEI CTNAG 7 cut(s) 276, 795, 876, 1045, 1142, 1580, 1671
BstF5I GGATG 4 cut(s) 504, 538, 547, 1669
BstFNI CGCG 1 cut(s) 1684
BstKTI GATC 9 cut(s) 15, 79, 373, 406, 411, 523, 781, 794, 1043
BstMBI GATC 9 cut(s) 12, 76, 370, 403, 408, 520, 778, 791, 1040
BstMWI GCNNNNNNNGC 5 cut(s) 326, 1031, 1427, 1517, 1526
BstNI CCWGG 1 cut(s) 264
BstNSI RCATGY 1 cut(s) 1138
BstSCI CCNGG 1 cut(s) 262
BstSFI CTRYAG 3 cut(s) 66, 974, 1173
BstSLI GKGCMC 1 cut(s) 1531
BstUI CGCG 1 cut(s) 1684
BstV1I GCAGC 3 cut(s) 304, 1330, 1414
BstV2I GAAGAC 1 cut(s) 629
BstX2I RGATCY 4 cut(s) 408, 520, 791, 1040
BstYI RGATCY 4 cut(s) 408, 520, 791, 1040
BsuRI GGCC 3 cut(s) 839, 1058, 1446
BtsCI GGATG 4 cut(s) 504, 538, 547, 1669
BtsIMutI CAGTG 8 cut(s) 22, 50, 75, 736, 816, 834, 1053, 1273
BveI ACCTGC 1 cut(s) 790
CaiI CAGNNNCTG 1 cut(s) 1181
Cfr13I GGNCC 2 cut(s) 1445, 1667
CseI GACGC 1 cut(s) 1673
Csp6I GTAC 5 cut(s) 129, 232, 420, 507, 1650
CviAII CATG 2 cut(s) 512, 1135
CviQI GTAC 5 cut(s) 129, 232, 420, 507, 1650
DdeI CTNAG 7 cut(s) 276, 795, 876, 1045, 1142, 1580, 1671
DpnI GATC 9 cut(s) 14, 78, 372, 405, 410, 522, 780, 793, 1042
DpnII GATC 9 cut(s) 12, 76, 370, 403, 408, 520, 778, 791, 1040
DraI TTTAAA 1 cut(s) 757
Eam1104I CTCTTC 2 cut(s) 782, 1290
EarI CTCTTC 2 cut(s) 782, 1290
Eco130I CCWWGG 1 cut(s) 1422
Eco24I GRGCYC 1 cut(s) 483
Eco47I GGWCC 1 cut(s) 1667
Eco57I CTGAAG 2 cut(s) 767, 1290
Eco88I CYCGRG 1 cut(s) 1447
EcoRI GAATTC 1 cut(s) 913
EcoRII CCWGG 1 cut(s) 262
EcoT14I CCWWGG 1 cut(s) 1422
EcoT38I GRGCYC 1 cut(s) 483
ErhI CCWWGG 1 cut(s) 1422
FaeI CATG 2 cut(s) 515, 1138
FaiI YATR 7 cut(s) 429, 488, 513, 554, 1136, 1211, 1361
FaqI GGGAC 1 cut(s) 1644
FatI CATG 2 cut(s) 511, 1134
FbaI TGATCA 1 cut(s) 12
Fnu4HI GCNGC 3 cut(s) 318, 1319, 1428
FokI GGATG 4 cut(s) 511, 545, 554, 1676
FriOI GRGCYC 1 cut(s) 483
Fsp4HI GCNGC 3 cut(s) 318, 1319, 1428
FspBI CTAG 8 cut(s) 456, 891, 924, 957, 990, 1077, 1194, 1655
GluI GCNGC 3 cut(s) 318, 1319, 1428
GsuI CTGGAG 2 cut(s) 732, 792
HaeIII GGCC 3 cut(s) 839, 1058, 1446
HapII CCGG 1 cut(s) 1020
HgaI GACGC 1 cut(s) 1673
Hin1II CATG 2 cut(s) 515, 1138
HincII GTYRAC 2 cut(s) 1273, 1485
HindII GTYRAC 2 cut(s) 1273, 1485
HinfI GANTC 7 cut(s) 134, 188, 625, 774, 880, 1588, 1619
HpaI GTTAAC 1 cut(s) 1485
HpaII CCGG 1 cut(s) 1020
HphI GGTGA 2 cut(s) 849, 1235
Hpy166II GTNNAC 3 cut(s) 1273, 1485, 1529
Hpy188I TCNGA 9 cut(s) 12, 279, 385, 408, 621, 786, 879, 885, 1165
Hpy188III TCNNGA 9 cut(s) 152, 247, 341, 476, 695, 749, 1252, 1406, 1655
Hpy8I GTNNAC 3 cut(s) 1273, 1485, 1529
Hpy99I CGWCG 1 cut(s) 1689
HpyAV CCTTC 6 cut(s) 31, 156, 662, 1048, 1314, 1471
HpyCH4III ACNGT 5 cut(s) 70, 731, 829, 1183, 1268
HpyCH4V TGCA 7 cut(s) 361, 976, 1034, 1132, 1318, 1520, 1529
HpyF10VI GCNNNNNNNGC 5 cut(s) 326, 1031, 1427, 1517, 1526
HpyF3I CTNAG 7 cut(s) 276, 795, 876, 1045, 1142, 1580, 1671
Hsp92II CATG 2 cut(s) 515, 1138
Ksp22I TGATCA 1 cut(s) 12
KspAI GTTAAC 1 cut(s) 1485
Kzo9I GATC 9 cut(s) 12, 76, 370, 403, 408, 520, 778, 791, 1040
LmnI GCTCC 8 cut(s) 334, 751, 1022, 1080, 1213, 1326, 1435, 1552
Lsp1109I GCAGC 3 cut(s) 304, 1330, 1414
LweI GCATC 2 cut(s) 91, 1507
MaeI CTAG 8 cut(s) 456, 891, 924, 957, 990, 1077, 1194, 1655
MaeIII GTNAC 6 cut(s) 802, 903, 1048, 1137, 1186, 1386
MalI GATC 9 cut(s) 14, 78, 372, 405, 410, 522, 780, 793, 1042
MboI GATC 9 cut(s) 12, 76, 370, 403, 408, 520, 778, 791, 1040
MboII GAAGA 9 cut(s) 251, 530, 634, 659, 773, 799, 856, 1307, 1469
MfeI CAATTG 1 cut(s) 1277
MflI RGATCY 4 cut(s) 408, 520, 791, 1040
MhlI GDGCHC 4 cut(s) 463, 483, 1085, 1531
MluCI AATT 6 cut(s) 432, 752, 852, 913, 1277, 1493
MluI ACGCGT 1 cut(s) 1682
MlyI GAGTC 2 cut(s) 619, 1597
MmeI TCCRAC 1 cut(s) 908
MseI TTAA 5 cut(s) 377, 756, 1073, 1484, 1593
MslI CAYNNNNRTG 1 cut(s) 1358
MspA1I CMGCKG 1 cut(s) 686
MspCI CTTAAG 1 cut(s) 1592
MspI CCGG 1 cut(s) 1020
MspR9I CCNGG 1 cut(s) 264
MunI CAATTG 1 cut(s) 1277
Mva1269I GAATGC 1 cut(s) 1247
MvaI CCWGG 1 cut(s) 264
MvnI CGCG 1 cut(s) 1684
MwoI GCNNNNNNNGC 5 cut(s) 326, 1031, 1427, 1517, 1526
NdeII GATC 9 cut(s) 12, 76, 370, 403, 408, 520, 778, 791, 1040
NlaIII CATG 2 cut(s) 515, 1138
NmeAIII GCCGAG 1 cut(s) 355
NmuCI GTSAC 3 cut(s) 1048, 1137, 1386
NspI RCATGY 1 cut(s) 1138
PciI ACATGT 1 cut(s) 1134
PctI GAATGC 1 cut(s) 1247
PfeI GAWTC 5 cut(s) 134, 188, 774, 880, 1619
PflFI GACNNNGTC 2 cut(s) 1235, 1587
PkrI GCNGC 3 cut(s) 319, 1320, 1429
PleI GAGTC 2 cut(s) 619, 1596
PpsI GAGTC 2 cut(s) 619, 1596
PscI ACATGT 1 cut(s) 1134
Psp6I CCWGG 1 cut(s) 262
PspGI CCWGG 1 cut(s) 262
PspPI GGNCC 2 cut(s) 1445, 1667
PstI CTGCAG 1 cut(s) 978
PstNI CAGNNNCTG 1 cut(s) 1181
PsuI RGATCY 4 cut(s) 408, 520, 791, 1040
PsyI GACNNNGTC 2 cut(s) 1235, 1587
PvuII CAGCTG 1 cut(s) 686
RsaI GTAC 5 cut(s) 130, 233, 421, 508, 1651
RsaNI GTAC 5 cut(s) 129, 232, 420, 507, 1650
RseI CAYNNNNRTG 1 cut(s) 1358
SaqAI TTAA 5 cut(s) 377, 756, 1073, 1484, 1593
SatI GCNGC 3 cut(s) 318, 1319, 1428
Sau3AI GATC 9 cut(s) 12, 76, 370, 403, 408, 520, 778, 791, 1040
Sau96I GGNCC 2 cut(s) 1445, 1667
ScaI AGTACT 3 cut(s) 233, 508, 1651
SchI GAGTC 2 cut(s) 619, 1597
ScrFI CCNGG 1 cut(s) 264
SduI GDGCHC 4 cut(s) 463, 483, 1085, 1531
SfaNI GCATC 2 cut(s) 91, 1507
SfcI CTRYAG 3 cut(s) 66, 974, 1173
SinI GGWCC 1 cut(s) 1667
SmiMI CAYNNNNRTG 1 cut(s) 1358
SmlI CTYRAG 4 cut(s) 462, 524, 693, 1592
SmoI CTYRAG 4 cut(s) 462, 524, 693, 1592
SpeI ACTAGT 1 cut(s) 455
Sse9I AATT 6 cut(s) 432, 752, 852, 913, 1277, 1493
SsiI CCGC 2 cut(s) 606, 941
SspMI CTAG 8 cut(s) 456, 891, 924, 957, 990, 1077, 1194, 1655
StyD4I CCNGG 1 cut(s) 262
StyI CCWWGG 1 cut(s) 1422
TaaI ACNGT 5 cut(s) 70, 731, 829, 1183, 1268
TaqI TCGA 5 cut(s) 246, 322, 777, 1334, 1643
TasI AATT 6 cut(s) 432, 752, 852, 913, 1277, 1493
TatI WGTACW 3 cut(s) 231, 506, 1649
TfiI GAWTC 5 cut(s) 134, 188, 774, 880, 1619
Tru1I TTAA 5 cut(s) 377, 756, 1073, 1484, 1593
Tru9I TTAA 5 cut(s) 377, 756, 1073, 1484, 1593
TscAI CASTG 8 cut(s) 22, 57, 75, 736, 816, 834, 1053, 1273
TseFI GTSAC 3 cut(s) 1048, 1137, 1386
TseI GCWGC 3 cut(s) 317, 1318, 1427
Tsp45I GTSAC 3 cut(s) 1048, 1137, 1386
TspDTI ATGAA 4 cut(s) 126, 180, 1056, 1308
TspGWI ACGGA 2 cut(s) 437, 1489
TspRI CASTG 8 cut(s) 22, 57, 75, 736, 816, 834, 1053, 1273
Tth111I GACNNNGTC 2 cut(s) 1235, 1587
Vha464I CTTAAG 1 cut(s) 1592
VneI GTGCAC 1 cut(s) 1527
VpaK11BI GGWCC 1 cut(s) 1667
XapI RAATTY 2 cut(s) 752, 913
XbaI TCTAGA 1 cut(s) 1654
XceI RCATGY 1 cut(s) 1138
XspI CTAG 8 cut(s) 456, 891, 924, 957, 990, 1077, 1194, 1655
ZrmI AGTACT 3 cut(s) 233, 508, 1651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.