MD13G1085000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0003792
Physical Location & Seq
Forward (+)
5971204 .. 5975047
3844 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1085000.v1.1.491

Sequence Viewer

Length: 1713 bp
ATGGGTGAATCAATGATGCATTTTGCCTATGATGCTGAAAAGGTGGTTGAGGGGGAGACGGTAGCTTCAAATTCTTCAAATCCTGTACTGGAAGTGTCGGTGTCTTTCGGAAAATTTGAGAATGATTCTTCACTGTCTTGGGAGAAATGGTCGACGTTTTCGCCGAATAAGTACTTGGAGGAAGTGGAGAAGTGTGCCACTCCTGGCTCAGTGGCCCAGAAAAGGGCTTACTTTGAAGCTCATTACAAGAGGGTTGCTGCTCGAAAAGCCGAGGAGATGATGGAACAGGAGAAGCAATTGCAAGATGGTGATACCTTTAGGTCGGATTCTGACCAGACGAGTGGAGATCAGATGGATTCTGAGGTGCATTTCGAAATTGATTTGGCCAACAATCAAATTAATGCCCAAGGAAATGATCAAGAAGCGAATTTCGACAATGAGATTATTAAAACTTACGTGGATGATCTCGAGGAGGATGATGTTTTCACCATAGAGTTTCCAAGCTCATTGGCTGAGGGAGAGAGAGAAGAAGCTGATTGCAGAACGGATAGTCCTGATTTGAACAATCCGGAGGATGAACTTGCTGTGGTGAAAGAAGTAGAAAATGTCCCTGCTGAATCTCAGGGAGTACAGGAGATTCCAAGGACTTTGGACAATGATGTAGGAAGTGCTCCAGAAGTTAAAGAGGTGCAAGAGGAAAAACCGAGATTGGATCTTCTGAAGGGATCTCCGAAGGTTACTCCATTGAGCAAGGAGAGAAATGTGGCAAACGTTAAGAAGAAACCAGTTCCACTAATAGCCAAAACACCACAGAAATTTGCCCCTAGGGCATCGAAGTCTGTATCAACTCCAACCCCTAAAGCATCGAAGCCCATATCAACTCCGACCCCTAAAGCATCGAAGCCCGTATCAACTCCGACCCCTAAAGCATCGAAGCCCGTATCAACTCCGACCCCTAAAGCATCGAAGCCTATATCAACTTCCACCCCTAGAGTATCGAAGTCTATATCAACTTCCACACCTAGAGTGTCAAAGTCTCTGTTAACCTCTACTGCAACTCCTGCATCGCGGTCCTCAGTAAAGAAGAGCAATGCTTCCTCTTTTCCTAGGAGCAAAAATCCTTCAACTGAGGAAACCAAGGAAGTTCCTCCCAAGCGTTTGCACTTGTCACTCAATGTGGAATCCAGTCCCACAATGTCTGGTTCAGCGTTTGTGACCACCACAACCAAAAAGTCTTCCATTATGGAGAATATGGGAGATAAGGACATCGTCAAACGAGCATTTAAGACATTTCAAAACACTTACAACCAACCGCAACCCTCCAGTGAAGAGAAACCGCCAACACCAAAGCAGCAGCTCTCTACGAAGGGAAAAGAATCAAGGGTTTCCACTTCTGCGCCCCTGACAAAAGAGAATGGAGGATATCTAGATAAAAGAACTGCTAAGGCCGCTCCATCATCTGTTAGCTTGAGAAGTGATGACTGCGTAGATAAAAGAGAGTTTTCACAGAAACTGGCGGCGAAATCCAATGCGAAAGTGACGGAGAAAGCACACTCCCAACCGAAGTCAAAGGATCAAAAGGAGGCAGAGATAAAAAAGCTCAGACATAGTCTTAATTTTAAGGCTACACCCACGCCATGCGTCTATCGGGGGCAAAAAGTATCAAAAAGTACTTCCGAAAAGGAGACTTCCAACAATGAAACCCATCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

571

Amino Acids

62.61

Weight (kDa)

8.49

Isoelectric Point (pI)

43.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 489 - 557 9.4e-13 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1451
AccI GTMKAC 1 cut(s) 152
AccII CGCG 1 cut(s) 1069
AccIII TCCGGA 1 cut(s) 568
AciI CCGC 5 cut(s) 1069, 1313, 1337, 1449, 1517
AclI AACGTT 1 cut(s) 771
AclWI GGATC 3 cut(s) 720, 733, 1581
AcoI YGGCCR 1 cut(s) 384
AcsI RAATTY 4 cut(s) 70, 113, 427, 815
AcuI CTGAAG 1 cut(s) 740
AfaI GTAC 4 cut(s) 87, 173, 630, 1672
AfiI CCNNNNNNNGG 2 cut(s) 222, 223
AgsI TTSAA 6 cut(s) 69, 78, 236, 562, 1125, 1295
AjnI CCWGG 1 cut(s) 202
AjuI GAANNNNNNNTTGG 2 cut(s) 158, 190
AloI GAACNNNNNNTCC 2 cut(s) 535, 567
AluBI AGCT 7 cut(s) 65, 239, 504, 533, 1357, 1467, 1600
AluI AGCT 7 cut(s) 65, 239, 504, 533, 1357, 1467, 1600
Alw21I GWGCWC 1 cut(s) 673
Alw26I GTCTC 3 cut(s) 50, 1041, 1679
AlwI GGATC 3 cut(s) 720, 733, 1581
AlwNI CAGNNNCTG 1 cut(s) 1513
Ama87I CYCGRG 1 cut(s) 467
Aor13HI TCCGGA 1 cut(s) 568
AoxI GGCC 3 cut(s) 213, 384, 1446
ApeKI GCWGC 3 cut(s) 257, 1351, 1354
ApoI RAATTY 4 cut(s) 70, 113, 427, 815
AseI ATTAAT 1 cut(s) 399
AspA2I CCTAGG 2 cut(s) 824, 1106
AspLEI GCGC 1 cut(s) 1399
AspS9I GGNCC 2 cut(s) 214, 1071
AsuHPI GGTGA 4 cut(s) 17, 320, 478, 601
AsuII TTCGAA 1 cut(s) 372
AvaI CYCGRG 1 cut(s) 467
AvaII GGWCC 1 cut(s) 1071
AvrII CCTAGG 2 cut(s) 824, 1106
BalI TGGCCA 1 cut(s) 386
BbsI GAAGAC 1 cut(s) 1227
Bbv12I GWGCWC 1 cut(s) 673
BbvCI CCTCAGC 1 cut(s) 513
BbvI GCAGC 3 cut(s) 244, 1363, 1366
BccI CCATC 4 cut(s) 274, 299, 346, 1462
BciT130I CCWGG 1 cut(s) 204
BclI TGATCA 1 cut(s) 415
BcoDI GTCTC 3 cut(s) 50, 1041, 1679
BfaI CTAG 5 cut(s) 825, 990, 1023, 1107, 1427
BglI GCCNNNNNGGC 1 cut(s) 827
BisI GCNGC 5 cut(s) 258, 1352, 1355, 1449, 1518
BlnI CCTAGG 2 cut(s) 824, 1106
BlsI GCNGC 5 cut(s) 259, 1353, 1356, 1450, 1519
BmcAI AGTACT 2 cut(s) 173, 1672
Bme1390I CCNGG 1 cut(s) 204
Bme18I GGWCC 1 cut(s) 1071
BmeT110I CYCGRG 1 cut(s) 467
BmgT120I GGNCC 2 cut(s) 214, 1071
BmrFI CCNGG 1 cut(s) 204
BmsI GCATC 8 cut(s) 6, 22, 839, 872, 905, 938, 971, 1073
BpiI GAAGAC 1 cut(s) 1227
BpmI CTGGAG 2 cut(s) 657, 1306
Bpu10I CCTNAGC 2 cut(s) 513, 1443
Bpu14I TTCGAA 1 cut(s) 372
BpuEI CTTGAG 1 cut(s) 1489
BsaAI YACGTR 1 cut(s) 457
BsaJI CCNNGG 6 cut(s) 270, 406, 641, 824, 1106, 1137
BsaWI WCCGGW 1 cut(s) 568
BsaXI ACNNNNNCTCC 4 cut(s) 134, 164, 1042, 1072
Bsc4I CCNNNNNNNGG 2 cut(s) 222, 223
Bse1I ACTGG 5 cut(s) 93, 785, 1185, 1323, 1518
Bse3DI GCAATG 1 cut(s) 1096
BseAI TCCGGA 1 cut(s) 568
BseBI CCWGG 1 cut(s) 204
BseDI CCNNGG 6 cut(s) 270, 406, 641, 824, 1106, 1137
BseGI GGATG 3 cut(s) 466, 481, 580
BseLI CCNNNNNNNGG 2 cut(s) 222, 223
BseMI GCAATG 1 cut(s) 1096
BseMII CTCAG 7 cut(s) 222, 351, 504, 635, 1089, 1119, 1615
BseNI ACTGG 5 cut(s) 93, 785, 1185, 1323, 1518
BseRI GAGGAG 2 cut(s) 287, 485
BseXI GCAGC 3 cut(s) 244, 1363, 1366
Bsh1236I CGCG 1 cut(s) 1069
BshFI GGCC 3 cut(s) 215, 386, 1448
BsiHKAI GWGCWC 1 cut(s) 673
BsiHKCI CYCGRG 1 cut(s) 467
BsiSI CCGG 1 cut(s) 569
BslFI GGGAC 2 cut(s) 593, 1173
BslI CCNNNNNNNGG 2 cut(s) 222, 223
BsmAI GTCTC 3 cut(s) 50, 1041, 1679
BsmBI CGTCTC 1 cut(s) 50
BsmFI GGGAC 2 cut(s) 593, 1173
BsnI GGCC 3 cut(s) 215, 386, 1448
BsoBI CYCGRG 1 cut(s) 467
Bsp119I TTCGAA 1 cut(s) 372
Bsp1286I GDGCHC 1 cut(s) 673
Bsp13I TCCGGA 1 cut(s) 568
Bsp143I GATC 6 cut(s) 346, 415, 463, 712, 725, 1573
BspACI CCGC 5 cut(s) 1069, 1313, 1337, 1449, 1517
BspANI GGCC 3 cut(s) 215, 386, 1448
BspCNI CTCAG 7 cut(s) 221, 352, 505, 634, 1088, 1120, 1614
BspEI TCCGGA 1 cut(s) 568
BspFNI CGCG 1 cut(s) 1069
BspPI GGATC 3 cut(s) 720, 733, 1581
BspQI GCTCTTC 1 cut(s) 1079
BspT104I TTCGAA 1 cut(s) 372
BsrBI CCGCTC 1 cut(s) 1451
BsrDI GCAATG 1 cut(s) 1096
BsrI ACTGG 5 cut(s) 93, 785, 1185, 1323, 1518
BssECI CCNNGG 6 cut(s) 270, 406, 641, 824, 1106, 1137
BssMI GATC 6 cut(s) 346, 415, 463, 712, 725, 1573
BssT1I CCWWGG 5 cut(s) 406, 641, 824, 1106, 1137
Bst2UI CCWGG 1 cut(s) 204
Bst4CI ACNGT 2 cut(s) 61, 135
Bst6I CTCTTC 2 cut(s) 1079, 1323
BstAPI GCANNNNNTGC 1 cut(s) 1061
BstBAI YACGTR 1 cut(s) 457
BstBI TTCGAA 1 cut(s) 372
BstDEI CTNAG 8 cut(s) 208, 360, 513, 621, 1075, 1128, 1443, 1601
BstF5I GGATG 3 cut(s) 466, 481, 580
BstFNI CGCG 1 cut(s) 1069
BstHHI GCGC 1 cut(s) 1399
BstKTI GATC 6 cut(s) 349, 418, 466, 715, 728, 1576
BstMAI GTCTC 3 cut(s) 50, 1041, 1679
BstMBI GATC 6 cut(s) 346, 415, 463, 712, 725, 1573
BstMWI GCNNNNNNNGC 5 cut(s) 32, 266, 827, 1061, 1448
BstNI CCWGG 1 cut(s) 204
BstSCI CCNGG 1 cut(s) 202
BstUI CGCG 1 cut(s) 1069
BstV1I GCAGC 3 cut(s) 244, 1363, 1366
BstV2I GAAGAC 1 cut(s) 1227
BstX2I RGATCY 2 cut(s) 712, 725
BstXI CCANNNNNNTGG 1 cut(s) 341
BstYI RGATCY 2 cut(s) 712, 725
BsuRI GGCC 3 cut(s) 215, 386, 1448
BtgZI GCGATG 1 cut(s) 1050
BtsCI GGATG 3 cut(s) 466, 481, 580
BtsIMutI CAGTG 3 cut(s) 131, 216, 1330
CaiI CAGNNNCTG 1 cut(s) 1513
CfoI GCGC 1 cut(s) 1399
Cfr13I GGNCC 2 cut(s) 214, 1071
CseI GACGC 1 cut(s) 1630
Csp6I GTAC 4 cut(s) 86, 172, 629, 1671
CviAII CATG 1 cut(s) 1638
CviQI GTAC 4 cut(s) 86, 172, 629, 1671
DdeI CTNAG 8 cut(s) 208, 360, 513, 621, 1075, 1128, 1443, 1601
DpnI GATC 6 cut(s) 348, 417, 465, 714, 727, 1575
DpnII GATC 6 cut(s) 346, 415, 463, 712, 725, 1573
EaeI YGGCCR 1 cut(s) 384
Eam1104I CTCTTC 2 cut(s) 1079, 1323
EarI CTCTTC 2 cut(s) 1079, 1323
Eco130I CCWWGG 5 cut(s) 406, 641, 824, 1106, 1137
Eco32I GATATC 1 cut(s) 1424
Eco47I GGWCC 1 cut(s) 1071
Eco57I CTGAAG 1 cut(s) 740
Eco88I CYCGRG 1 cut(s) 467
EcoRII CCWGG 1 cut(s) 202
EcoRV GATATC 1 cut(s) 1424
EcoT14I CCWWGG 5 cut(s) 406, 641, 824, 1106, 1137
EcoT22I ATGCAT 1 cut(s) 21
ErhI CCWWGG 5 cut(s) 406, 641, 824, 1106, 1137
Esp3I CGTCTC 1 cut(s) 50
FaeI CATG 1 cut(s) 1641
FaiI YATR 9 cut(s) 30, 491, 875, 974, 1007, 1244, 1253, 1608, 1639
FaqI GGGAC 2 cut(s) 593, 1173
FatI CATG 1 cut(s) 1637
FbaI TGATCA 1 cut(s) 415
FblI GTMKAC 1 cut(s) 152
Fnu4HI GCNGC 5 cut(s) 258, 1352, 1355, 1449, 1518
FokI GGATG 3 cut(s) 473, 488, 587
Fsp4HI GCNGC 5 cut(s) 258, 1352, 1355, 1449, 1518
FspBI CTAG 5 cut(s) 825, 990, 1023, 1107, 1427
GlaI GCGC 1 cut(s) 1398
GluI GCNGC 5 cut(s) 258, 1352, 1355, 1449, 1518
GsuI CTGGAG 2 cut(s) 657, 1306
HaeIII GGCC 3 cut(s) 215, 386, 1448
HapII CCGG 1 cut(s) 569
HgaI GACGC 1 cut(s) 1630
HhaI GCGC 1 cut(s) 1399
Hin1II CATG 1 cut(s) 1641
Hin6I GCGC 1 cut(s) 1397
HinP1I GCGC 1 cut(s) 1397
HincII GTYRAC 2 cut(s) 153, 1044
HindII GTYRAC 2 cut(s) 153, 1044
HinfI GANTC 8 cut(s) 8, 125, 326, 356, 617, 637, 1181, 1376
HpaI GTTAAC 1 cut(s) 1044
HpaII CCGG 1 cut(s) 569
HphI GGTGA 4 cut(s) 17, 320, 478, 601
Hpy166II GTNNAC 2 cut(s) 153, 1044
Hpy188III TCNNGA 6 cut(s) 419, 467, 554, 569, 674, 1427
Hpy8I GTNNAC 2 cut(s) 153, 1044
Hpy99I CGWCG 1 cut(s) 157
HpyAV CCTTC 4 cut(s) 715, 727, 1131, 1360
HpyCH4III ACNGT 2 cut(s) 61, 135
HpyCH4IV ACGT 3 cut(s) 155, 456, 771
HpyCH4V TGCA 8 cut(s) 19, 301, 367, 540, 691, 1055, 1064, 1162
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 266, 827, 1061, 1448
HpyF3I CTNAG 8 cut(s) 208, 360, 513, 621, 1075, 1128, 1443, 1601
HpySE526I ACGT 3 cut(s) 155, 456, 771
Hsp92II CATG 1 cut(s) 1641
HspAI GCGC 1 cut(s) 1397
Kpn2I TCCGGA 1 cut(s) 568
Ksp22I TGATCA 1 cut(s) 415
KspAI GTTAAC 1 cut(s) 1044
Kzo9I GATC 6 cut(s) 346, 415, 463, 712, 725, 1573
LguI GCTCTTC 1 cut(s) 1079
LmnI GCTCC 3 cut(s) 676, 1110, 1456
Lsp1109I GCAGC 3 cut(s) 244, 1363, 1366
LweI GCATC 8 cut(s) 6, 22, 839, 872, 905, 938, 971, 1073
MaeI CTAG 5 cut(s) 825, 990, 1023, 1107, 1427
MaeII ACGT 3 cut(s) 155, 456, 771
MaeIII GTNAC 4 cut(s) 736, 1167, 1213, 1537
MalI GATC 6 cut(s) 348, 417, 465, 714, 727, 1575
MbiI CCGCTC 1 cut(s) 1451
MboI GATC 6 cut(s) 346, 415, 463, 712, 725, 1573
MboII GAAGA 8 cut(s) 66, 120, 539, 707, 790, 1096, 1227, 1340
MfeI CAATTG 1 cut(s) 296
MflI RGATCY 2 cut(s) 712, 725
MhlI GDGCHC 1 cut(s) 673
MlsI TGGCCA 1 cut(s) 386
MluCI AATT 8 cut(s) 70, 113, 296, 375, 396, 427, 815, 1615
MluNI TGGCCA 1 cut(s) 386
MmeI TCCRAC 5 cut(s) 303, 875, 908, 941, 974
Mox20I TGGCCA 1 cut(s) 386
Mph1103I ATGCAT 1 cut(s) 21
MroI TCCGGA 1 cut(s) 568
MscI TGGCCA 1 cut(s) 386
MseI TTAA 8 cut(s) 399, 447, 681, 774, 1043, 1284, 1614, 1620
Msp20I TGGCCA 1 cut(s) 386
MspI CCGG 1 cut(s) 569
MspR9I CCNGG 1 cut(s) 204
MunI CAATTG 1 cut(s) 296
MvaI CCWGG 1 cut(s) 204
MvnI CGCG 1 cut(s) 1069
MwoI GCNNNNNNNGC 5 cut(s) 32, 266, 827, 1061, 1448
NdeII GATC 6 cut(s) 346, 415, 463, 712, 725, 1573
NlaIII CATG 1 cut(s) 1641
NmeAIII GCCGAG 1 cut(s) 295
NmuCI GTSAC 3 cut(s) 1167, 1213, 1537
NsiI ATGCAT 1 cut(s) 21
NspV TTCGAA 1 cut(s) 372
PaeR7I CTCGAG 1 cut(s) 467
PciSI GCTCTTC 1 cut(s) 1079
PcsI WCGNNNNNNNCGW 1 cut(s) 161
PfeI GAWTC 8 cut(s) 8, 125, 326, 356, 617, 637, 1181, 1376
PflFI GACNNNGTC 2 cut(s) 1268, 1608
PkrI GCNGC 5 cut(s) 259, 1353, 1356, 1450, 1519
Ppu21I YACGTR 1 cut(s) 457
PshBI ATTAAT 1 cut(s) 399
Psp1406I AACGTT 1 cut(s) 771
Psp6I CCWGG 1 cut(s) 202
PspGI CCWGG 1 cut(s) 202
PspPI GGNCC 2 cut(s) 214, 1071
PstNI CAGNNNCTG 1 cut(s) 1513
PsuI RGATCY 2 cut(s) 712, 725
PsyI GACNNNGTC 2 cut(s) 1268, 1608
RsaI GTAC 4 cut(s) 87, 173, 630, 1672
RsaNI GTAC 4 cut(s) 86, 172, 629, 1671
SalI GTCGAC 1 cut(s) 151
SapI GCTCTTC 1 cut(s) 1079
SaqAI TTAA 8 cut(s) 399, 447, 681, 774, 1043, 1284, 1614, 1620
SatI GCNGC 5 cut(s) 258, 1352, 1355, 1449, 1518
Sau3AI GATC 6 cut(s) 346, 415, 463, 712, 725, 1573
Sau96I GGNCC 2 cut(s) 214, 1071
ScaI AGTACT 2 cut(s) 173, 1672
ScrFI CCNGG 1 cut(s) 204
SduI GDGCHC 1 cut(s) 673
SfaNI GCATC 8 cut(s) 6, 22, 839, 872, 905, 938, 971, 1073
Sfr274I CTCGAG 1 cut(s) 467
SfuI TTCGAA 1 cut(s) 372
SinI GGWCC 1 cut(s) 1071
SlaI CTCGAG 1 cut(s) 467
SmlI CTYRAG 2 cut(s) 467, 1468
SmoI CTYRAG 2 cut(s) 467, 1468
Sse9I AATT 8 cut(s) 70, 113, 296, 375, 396, 427, 815, 1615
SsiI CCGC 5 cut(s) 1069, 1313, 1337, 1449, 1517
SspMI CTAG 5 cut(s) 825, 990, 1023, 1107, 1427
StyD4I CCNGG 1 cut(s) 202
StyI CCWWGG 5 cut(s) 406, 641, 824, 1106, 1137
TaaI ACNGT 2 cut(s) 61, 135
TaiI ACGT 3 cut(s) 158, 459, 774
TasI AATT 8 cut(s) 70, 113, 296, 375, 396, 427, 815, 1615
TatI WGTACW 4 cut(s) 85, 171, 628, 1670
TauI GCSGC 2 cut(s) 1451, 1520
TfiI GAWTC 8 cut(s) 8, 125, 326, 356, 617, 637, 1181, 1376
Tru1I TTAA 8 cut(s) 399, 447, 681, 774, 1043, 1284, 1614, 1620
Tru9I TTAA 8 cut(s) 399, 447, 681, 774, 1043, 1284, 1614, 1620
TscAI CASTG 3 cut(s) 138, 216, 1330
TseFI GTSAC 3 cut(s) 1167, 1213, 1537
TseI GCWGC 3 cut(s) 257, 1351, 1354
Tsp45I GTSAC 3 cut(s) 1167, 1213, 1537
TspDTI ATGAA 2 cut(s) 591, 1713
TspGWI ACGGA 2 cut(s) 560, 1556
TspRI CASTG 3 cut(s) 138, 216, 1330
Tth111I GACNNNGTC 2 cut(s) 1268, 1608
VpaK11BI GGWCC 1 cut(s) 1071
VspI ATTAAT 1 cut(s) 399
XapI RAATTY 4 cut(s) 70, 113, 427, 815
XbaI TCTAGA 1 cut(s) 1426
XhoI CTCGAG 1 cut(s) 467
XmaJI CCTAGG 2 cut(s) 824, 1106
XmiI GTMKAC 1 cut(s) 152
XspI CTAG 5 cut(s) 825, 990, 1023, 1107, 1427
ZrmI AGTACT 2 cut(s) 173, 1672
Zsp2I ATGCAT 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.