FvH4_4g35241

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003792
Physical Location & Seq
Reverse (-)
32602982 .. 32608284
5303 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g35241.t1

Sequence Viewer

Length: 1674 bp
ATGGGTGAATCTATAGTGGGTTCTCCAAAAGATGCTGATAAGATGGGAGAGGTAGCTAGTACAGATTCGTCAAACCCTTCTCTGGAAGTGTCGGTTTCGTTTGGTAGGTTTGAGAATGATTCACTATCTTGGGAGAAATGGTCGGCATTTTCGCCAAATAAGTACTTGGAAGAAGTTGAGAAGTGTGCTACTCCTGGTTCAGTAGCACAGAAAAAGGCTTATTTTGAAGCTCATTACAAGAGGATAGCTGCTCGAAAGGCCGAGGAACTTCTGGAACAAGAGAAGCAAATGCATGATGATGAGCCCTTAAAGTCAGATGATCAAAATAATGGTGATCAGATCTGTTGTGGCACGGATAATGGAATTGATATTGATATAGCTACCAGTCAAACTAATGCTCAAGGAAATAGTCAGGAGCCCAATTTAGAAAATGGGATAAGCTGTACTCCTGTGGAAGATCTCAAGGAGGATGATGAGGATGTTTACACTATAGAGTGTCAAACATCATCGATTGAGGAAAGAGAGAGAGAGGAAACTGATAGCGGAGTAGTTAGTCCCAAGACTCCAAACTTGAACAGACCAGAGGAGCTTGTTTTGGTAAAAGAAGTGGAAACTATCACTGCTGACACTCAAGAAACAATACAGGAACTTACAAAGACTTTGGACAATGATGCAGGCGATGCTCCAGAAGTTAAAGAGGAAAAGGCGAGATTGGATCTTCAGAAGCGACCTCAGAAGGTAACTCCAGTGAGCAAGGAGAGGATGACTGTGGCAAAGGCCAAGAAGAAATCAGTTTCACCAATGACTAAAACACCTCAGAATCCAACCCCTAGAGTGTCAAAGTTACCACAGAATTCCACCCCTAGAGTGTCAAAGTTACCACAGAATTCTACCTCTAGAGTGTCAAAAACACCACAGAATTCCACCCCTAGAGTGTCAAAGATACCGCAGAATACTACCCCTAGAGTGTCAAAGATACTGCAGAATACCACCCCTAGAGTGTCAAAGCCAATGTCAGCTTCTACCGGAGCAAAATCTGCACCACGGCTCTCAGTTACGAATGCCAATGGTTCATCTTTAAGTAGGAGCTCAAATCCGTCTATTCAAAGAACCAAGAAAGTTCCTCCTAAATCTCTGCACATGTCACTCAGTTTGGATCCTAAAAAGTCTGATTCAGCTACAGAAACTGTGGTTACAGCTAGAAAATCTTTGATTATGGAGCAAATGGGTGACAAGGACATTGTCAAACGAGCATTTAAGACATTTCAAAACAGTGTCAACCAATTGAAATCCTCTAATGAAGAGAAACCTTCAACACCAAAGCAGCCCTCTACTAAGGCGAAAGAACCAAAGGTTTCCACTTCTGTGTCTCTGCCCAAAGACAATGGAGGGTCACTCAAAACGAGTTATCATGATAAAAGAAATGCTAAGGCTGCTCCATCTTTTGGCTTGAGAAGTGAAGAAAGAGCAGAGAAAAAGGAGTTAACAAATAAATTGGCAAAACCCAATGCCAGAGATGCAGAGAGAACACATTCCCAACCAAAGTCAAAGGAGCAAAAGGAGGCAGAGATAAAAATGCTAAGACAGAGTCTTAAGCTCAAGGCTACACCAACGACTGATTCTTATCAGGGACAAAAGTTATTGAAAAGCACTTCAGAAAAAGGAGGGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

558

Amino Acids

61.27

Weight (kDa)

9.07

Isoelectric Point (pI)

45.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 482 - 543 1.2e-08 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1445
AciI CCGC 2 cut(s) 543, 947
AclWI GGATC 3 cut(s) 723, 1151, 1164
AcsI RAATTY 3 cut(s) 853, 886, 919
AcuI CTGAAG 2 cut(s) 704, 1638
AfaI GTAC 3 cut(s) 61, 164, 445
AfiI CCNNNNNNNGG 2 cut(s) 82, 1445
AflII CTTAAG 1 cut(s) 1592
AflIII ACRYGT 1 cut(s) 1140
AgsI TTSAA 7 cut(s) 227, 574, 1106, 1268, 1288, 1314, 1645
AjnI CCWGG 1 cut(s) 193
AjuI GAANNNNNNNTTGG 4 cut(s) 1003, 1035, 1603, 1635
AleI CACNNNNGTG 1 cut(s) 1364
Alw21I GWGCWC 1 cut(s) 1091
Alw26I GTCTC 1 cut(s) 1374
AlwI GGATC 3 cut(s) 723, 1151, 1164
AlwNI CAGNNNCTG 1 cut(s) 1187
AoxI GGCC 2 cut(s) 258, 777
ApeKI GCWGC 3 cut(s) 248, 1324, 1433
ApoI RAATTY 3 cut(s) 853, 886, 919
ArsI GACNNNNNNTTYG 2 cut(s) 998, 1030
Asp700I GAANNNNTTC 1 cut(s) 1531
AspS9I GGNCC 1 cut(s) 1668
AsuHPI GGTGA 4 cut(s) 17, 344, 789, 1241
AvaII GGWCC 1 cut(s) 1668
BamHI GGATCC 1 cut(s) 1156
BanII GRGCYC 3 cut(s) 306, 420, 1091
Bbv12I GWGCWC 1 cut(s) 1091
BbvI GCAGC 3 cut(s) 235, 1336, 1420
BccI CCATC 2 cut(s) 37, 1447
BceAI ACGGC 1 cut(s) 1061
BciT130I CCWGG 1 cut(s) 195
BclI TGATCA 2 cut(s) 319, 334
BcoDI GTCTC 1 cut(s) 1374
BfaI CTAG 8 cut(s) 57, 831, 864, 897, 930, 963, 996, 1200
BfmI CTRYAG 4 cut(s) 12, 489, 980, 1179
BfrI CTTAAG 1 cut(s) 1592
BglII AGATCT 2 cut(s) 339, 457
BisI GCNGC 3 cut(s) 249, 1325, 1434
BlsI GCNGC 3 cut(s) 250, 1326, 1435
BmcAI AGTACT 1 cut(s) 164
Bme1390I CCNGG 1 cut(s) 195
Bme18I GGWCC 1 cut(s) 1668
BmgT120I GGNCC 1 cut(s) 1668
BmiI GGNNCC 3 cut(s) 417, 1158, 1669
BmrFI CCNGG 1 cut(s) 195
BmsI GCATC 4 cut(s) 22, 661, 670, 1507
BplI GAGNNNNNCTC 2 cut(s) 1380, 1412
BpmI CTGGAG 2 cut(s) 669, 729
Bpu10I CCTNAGC 1 cut(s) 1428
BpuEI CTTGAG 5 cut(s) 384, 446, 615, 1471, 1583
Bsa29I ATCGAT 1 cut(s) 509
BsaBI GATNNNNATC 1 cut(s) 1623
BsaJI CCNNGG 2 cut(s) 261, 1043
BsaWI WCCGGW 1 cut(s) 1025
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 2 cut(s) 82, 1445
Bse1I ACTGG 2 cut(s) 384, 746
Bse8I GATNNNNATC 1 cut(s) 1623
BseBI CCWGG 1 cut(s) 195
BseCI ATCGAT 1 cut(s) 509
BseDI CCNNGG 2 cut(s) 261, 1043
BseGI GGATG 3 cut(s) 475, 484, 768
BseJI GATNNNNATC 1 cut(s) 1623
BseLI CCNNNNNNNGG 2 cut(s) 82, 1445
BseMII CTCAG 4 cut(s) 746, 830, 1065, 1162
BseNI ACTGG 2 cut(s) 384, 746
BseRI GAGGAG 1 cut(s) 599
BseXI GCAGC 3 cut(s) 235, 1336, 1420
BsgI GTGCAG 2 cut(s) 1023, 1121
BshFI GGCC 2 cut(s) 260, 779
BshVI ATCGAT 1 cut(s) 509
BsiHKAI GWGCWC 1 cut(s) 1091
BsiSI CCGG 1 cut(s) 1026
BslFI GGGAC 2 cut(s) 540, 1644
BslI CCNNNNNNNGG 2 cut(s) 82, 1445
BsmAI GTCTC 1 cut(s) 1374
BsmFI GGGAC 2 cut(s) 540, 1644
BsmI GAATGC 1 cut(s) 1066
BsnI GGCC 2 cut(s) 260, 779
Bsp1286I GDGCHC 3 cut(s) 306, 420, 1091
Bsp143I GATC 6 cut(s) 319, 334, 339, 457, 715, 1156
BspACI CCGC 2 cut(s) 543, 947
BspANI GGCC 2 cut(s) 260, 779
BspCNI CTCAG 4 cut(s) 745, 829, 1064, 1161
BspDI ATCGAT 1 cut(s) 509
BspHI TCATGA 1 cut(s) 1411
BspLI GGNNCC 3 cut(s) 417, 1158, 1669
BspMAI CTGCAG 1 cut(s) 984
BspPI GGATC 3 cut(s) 723, 1151, 1164
BspTI CTTAAG 1 cut(s) 1592
BsrI ACTGG 2 cut(s) 384, 746
BssECI CCNNGG 2 cut(s) 261, 1043
BssMI GATC 6 cut(s) 319, 334, 339, 457, 715, 1156
Bst2UI CCWGG 1 cut(s) 195
Bst4CI ACNGT 3 cut(s) 769, 1189, 1274
Bst6I CTCTTC 1 cut(s) 1296
BstAFI CTTAAG 1 cut(s) 1592
BstAPI GCANNNNNTGC 2 cut(s) 680, 1037
BstC8I GCNNGC 1 cut(s) 676
BstDEI CTNAG 7 cut(s) 732, 816, 1051, 1148, 1335, 1428, 1580
BstDSI CCRYGG 1 cut(s) 1043
BstF5I GGATG 3 cut(s) 475, 484, 768
BstKTI GATC 6 cut(s) 322, 337, 342, 460, 718, 1159
BstMAI GTCTC 1 cut(s) 1374
BstMBI GATC 6 cut(s) 319, 334, 339, 457, 715, 1156
BstMWI GCNNNNNNNGC 5 cut(s) 257, 680, 1037, 1433, 1517
BstNI CCWGG 1 cut(s) 195
BstNSI RCATGY 1 cut(s) 1144
BstSCI CCNGG 1 cut(s) 193
BstSFI CTRYAG 4 cut(s) 12, 489, 980, 1179
BstV1I GCAGC 3 cut(s) 235, 1336, 1420
BstX2I RGATCY 4 cut(s) 339, 457, 715, 1156
BstYI RGATCY 4 cut(s) 339, 457, 715, 1156
Bsu15I ATCGAT 1 cut(s) 509
BsuRI GGCC 2 cut(s) 260, 779
BsuTUI ATCGAT 1 cut(s) 509
BtgI CCRYGG 1 cut(s) 1043
BtgZI GCGATG 1 cut(s) 693
BtsCI GGATG 3 cut(s) 475, 484, 768
BtsI GCAGTG 1 cut(s) 618
BtsIMutI CAGTG 3 cut(s) 618, 753, 1279
Cac8I GCNNGC 1 cut(s) 676
CaiI CAGNNNCTG 1 cut(s) 1187
CciI TCATGA 1 cut(s) 1411
Cfr13I GGNCC 1 cut(s) 1668
ClaI ATCGAT 1 cut(s) 509
Csp6I GTAC 3 cut(s) 60, 163, 444
CviAII CATG 3 cut(s) 293, 1141, 1412
CviQI GTAC 3 cut(s) 60, 163, 444
DdeI CTNAG 7 cut(s) 732, 816, 1051, 1148, 1335, 1428, 1580
DpnI GATC 6 cut(s) 321, 336, 341, 459, 717, 1158
DpnII GATC 6 cut(s) 319, 334, 339, 457, 715, 1156
Eam1104I CTCTTC 1 cut(s) 1296
EarI CTCTTC 1 cut(s) 1296
Ecl136II GAGCTC 1 cut(s) 1089
Eco24I GRGCYC 3 cut(s) 306, 420, 1091
Eco47I GGWCC 1 cut(s) 1668
Eco53kI GAGCTC 1 cut(s) 1089
Eco57I CTGAAG 2 cut(s) 704, 1638
EcoICRI GAGCTC 1 cut(s) 1089
EcoO109I RGGNCCY 1 cut(s) 1668
EcoRI GAATTC 3 cut(s) 853, 886, 919
EcoRII CCWGG 1 cut(s) 193
EcoT22I ATGCAT 1 cut(s) 294
EcoT38I GRGCYC 3 cut(s) 306, 420, 1091
FaeI CATG 3 cut(s) 296, 1144, 1415
FaiI YATR 7 cut(s) 14, 294, 377, 491, 1142, 1217, 1413
FaqI GGGAC 2 cut(s) 540, 1644
FatI CATG 3 cut(s) 292, 1140, 1411
FbaI TGATCA 2 cut(s) 319, 334
Fnu4HI GCNGC 3 cut(s) 249, 1325, 1434
FokI GGATG 3 cut(s) 482, 491, 775
FriOI GRGCYC 3 cut(s) 306, 420, 1091
Fsp4HI GCNGC 3 cut(s) 249, 1325, 1434
FspBI CTAG 8 cut(s) 57, 831, 864, 897, 930, 963, 996, 1200
GluI GCNGC 3 cut(s) 249, 1325, 1434
GsuI CTGGAG 2 cut(s) 669, 729
HaeIII GGCC 2 cut(s) 260, 779
HapII CCGG 1 cut(s) 1026
Hin1II CATG 3 cut(s) 296, 1144, 1415
HincII GTYRAC 2 cut(s) 1279, 1485
HindII GTYRAC 2 cut(s) 1279, 1485
HinfI GANTC 8 cut(s) 8, 65, 119, 562, 820, 1172, 1588, 1619
HpaI GTTAAC 1 cut(s) 1485
HpaII CCGG 1 cut(s) 1026
HphI GGTGA 4 cut(s) 17, 344, 789, 1241
Hpy166II GTNNAC 3 cut(s) 484, 1279, 1485
Hpy188I TCNGA 7 cut(s) 316, 339, 723, 735, 819, 1171, 1657
Hpy188III TCNNGA 7 cut(s) 83, 272, 413, 632, 686, 897, 1412
Hpy8I GTNNAC 3 cut(s) 484, 1279, 1485
HpyAV CCTTC 3 cut(s) 87, 730, 1320
HpyCH4III ACNGT 3 cut(s) 769, 1189, 1274
HpyCH4V TGCA 6 cut(s) 292, 674, 982, 1040, 1138, 1520
HpyF10VI GCNNNNNNNGC 5 cut(s) 257, 680, 1037, 1433, 1517
HpyF3I CTNAG 7 cut(s) 732, 816, 1051, 1148, 1335, 1428, 1580
Hsp92II CATG 3 cut(s) 296, 1144, 1415
Ksp22I TGATCA 2 cut(s) 319, 334
KspAI GTTAAC 1 cut(s) 1485
Kzo9I GATC 6 cut(s) 319, 334, 339, 457, 715, 1156
LmnI GCTCC 8 cut(s) 415, 586, 688, 1028, 1086, 1219, 1441, 1552
Lsp1109I GCAGC 3 cut(s) 235, 1336, 1420
LweI GCATC 4 cut(s) 22, 661, 670, 1507
MaeI CTAG 8 cut(s) 57, 831, 864, 897, 930, 963, 996, 1200
MaeIII GTNAC 8 cut(s) 739, 843, 876, 1054, 1143, 1192, 1229, 1392
MalI GATC 6 cut(s) 321, 336, 341, 459, 717, 1158
MboI GATC 6 cut(s) 319, 334, 339, 457, 715, 1156
MboII GAAGA 6 cut(s) 182, 467, 710, 796, 1313, 1472
MfeI CAATTG 1 cut(s) 1283
MflI RGATCY 4 cut(s) 339, 457, 715, 1156
MhlI GDGCHC 3 cut(s) 306, 420, 1091
MluCI AATT 7 cut(s) 363, 421, 853, 886, 919, 1283, 1493
MlyI GAGTC 2 cut(s) 556, 1597
MmeI TCCRAC 1 cut(s) 848
Mph1103I ATGCAT 1 cut(s) 294
MroXI GAANNNNTTC 1 cut(s) 1531
MseI TTAA 6 cut(s) 308, 693, 1079, 1257, 1484, 1593
MslI CAYNNNNRTG 2 cut(s) 297, 1364
MspCI CTTAAG 1 cut(s) 1592
MspI CCGG 1 cut(s) 1026
MspR9I CCNGG 1 cut(s) 195
MunI CAATTG 1 cut(s) 1283
Mva1269I GAATGC 1 cut(s) 1066
MvaI CCWGG 1 cut(s) 195
MwoI GCNNNNNNNGC 5 cut(s) 257, 680, 1037, 1433, 1517
NdeII GATC 6 cut(s) 319, 334, 339, 457, 715, 1156
NlaIII CATG 3 cut(s) 296, 1144, 1415
NlaIV GGNNCC 3 cut(s) 417, 1158, 1669
NmeAIII GCCGAG 1 cut(s) 286
NmuCI GTSAC 3 cut(s) 1143, 1229, 1392
NsiI ATGCAT 1 cut(s) 294
NspI RCATGY 1 cut(s) 1144
OliI CACNNNNGTG 1 cut(s) 1364
PagI TCATGA 1 cut(s) 1411
PciI ACATGT 1 cut(s) 1140
PctI GAATGC 1 cut(s) 1066
PdmI GAANNNNTTC 1 cut(s) 1531
PfeI GAWTC 6 cut(s) 8, 65, 119, 820, 1172, 1619
PflFI GACNNNGTC 2 cut(s) 1241, 1587
PflMI CCANNNNNTGG 1 cut(s) 1445
PkrI GCNGC 3 cut(s) 250, 1326, 1435
PleI GAGTC 2 cut(s) 556, 1596
PpsI GAGTC 2 cut(s) 556, 1596
PpuMI RGGWCCY 1 cut(s) 1668
PscI ACATGT 1 cut(s) 1140
Psp124BI GAGCTC 1 cut(s) 1091
Psp5II RGGWCCY 1 cut(s) 1668
Psp6I CCWGG 1 cut(s) 193
PspGI CCWGG 1 cut(s) 193
PspN4I GGNNCC 3 cut(s) 417, 1158, 1669
PspPI GGNCC 1 cut(s) 1668
PspPPI RGGWCCY 1 cut(s) 1668
PstI CTGCAG 1 cut(s) 984
PstNI CAGNNNCTG 1 cut(s) 1187
PsuI RGATCY 4 cut(s) 339, 457, 715, 1156
PsyI GACNNNGTC 2 cut(s) 1241, 1587
RsaI GTAC 3 cut(s) 61, 164, 445
RsaNI GTAC 3 cut(s) 60, 163, 444
RseI CAYNNNNRTG 2 cut(s) 297, 1364
SacI GAGCTC 1 cut(s) 1091
SaqAI TTAA 6 cut(s) 308, 693, 1079, 1257, 1484, 1593
SatI GCNGC 3 cut(s) 249, 1325, 1434
Sau3AI GATC 6 cut(s) 319, 334, 339, 457, 715, 1156
Sau96I GGNCC 1 cut(s) 1668
ScaI AGTACT 1 cut(s) 164
SchI GAGTC 2 cut(s) 556, 1597
ScrFI CCNGG 1 cut(s) 195
SduI GDGCHC 3 cut(s) 306, 420, 1091
SfaNI GCATC 4 cut(s) 22, 661, 670, 1507
SfcI CTRYAG 4 cut(s) 12, 489, 980, 1179
SinI GGWCC 1 cut(s) 1668
SmiMI CAYNNNNRTG 2 cut(s) 297, 1364
SmlI CTYRAG 6 cut(s) 399, 461, 630, 1450, 1592, 1598
SmoI CTYRAG 6 cut(s) 399, 461, 630, 1450, 1592, 1598
Sse9I AATT 7 cut(s) 363, 421, 853, 886, 919, 1283, 1493
SsiI CCGC 2 cut(s) 543, 947
SspMI CTAG 8 cut(s) 57, 831, 864, 897, 930, 963, 996, 1200
SstI GAGCTC 1 cut(s) 1091
StyD4I CCNGG 1 cut(s) 193
TaaI ACNGT 3 cut(s) 769, 1189, 1274
TaqI TCGA 2 cut(s) 253, 509
TasI AATT 7 cut(s) 363, 421, 853, 886, 919, 1283, 1493
TatI WGTACW 3 cut(s) 59, 162, 443
TfiI GAWTC 6 cut(s) 8, 65, 119, 820, 1172, 1619
Tru1I TTAA 6 cut(s) 308, 693, 1079, 1257, 1484, 1593
Tru9I TTAA 6 cut(s) 308, 693, 1079, 1257, 1484, 1593
TscAI CASTG 3 cut(s) 625, 753, 1279
TseFI GTSAC 3 cut(s) 1143, 1229, 1392
TseI GCWGC 3 cut(s) 248, 1324, 1433
Tsp45I GTSAC 3 cut(s) 1143, 1229, 1392
TspDTI ATGAA 2 cut(s) 1062, 1314
TspGWI ACGGA 2 cut(s) 368, 1086
TspRI CASTG 3 cut(s) 625, 753, 1279
Tth111I GACNNNGTC 2 cut(s) 1241, 1587
Van91I CCANNNNNTGG 1 cut(s) 1445
Vha464I CTTAAG 1 cut(s) 1592
VpaK11BI GGWCC 1 cut(s) 1668
XapI RAATTY 3 cut(s) 853, 886, 919
XbaI TCTAGA 1 cut(s) 896
XceI RCATGY 1 cut(s) 1144
XmnI GAANNNNTTC 1 cut(s) 1531
XspI CTAG 8 cut(s) 57, 831, 864, 897, 930, 963, 996, 1200
ZrmI AGTACT 1 cut(s) 164
Zsp2I ATGCAT 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.