MD16G1084200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0003792
Physical Location & Seq
Forward (+)
5912798 .. 5916917
4120 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1084200.v1.1.491

Sequence Viewer

Length: 1704 bp
ATGGGTGAATCAATAGTCGATTTTGCCAGGGATGCTGAAAAGGTGGTGGAGAGGGAGACATTAGCTTCAAATTCTTCAAACCCTCATCTGGAAGTGTCGGTTTCTTTCGGAAGATTTGAGAATGATTCTTCACTGTCCTGGGAGAAATGGTCGACATTTTCGCAGAATAAGTACTTGGAGGAAGTTGAGAAATGTGCCACTCCTGGCTCGGTGGCCCAGAAAAGGGCTTACTTTGAAGCCCATTACAAGAGGATTGCTGCTCGAAAAGCCGAGGAGTTGATGGAGCAAGAGAAGCAAATGCAAGGTGGTGATACCTTTAGGTCGGAATCTGATCAGAAGAGCGGAGATCAGATGGATTCCGAGGCTCATTTCGAAATTGATTTGGCTAACAGTCAAAGTAATGCCCAAGGAAATGATCGAGAAGCGAATTTCGACAGCGAGATTAGTGAAACTCATGTGGATGATCTCAAGGAGGATGATGTTTTTGCCATAGTGTGTCAAAACTCATTAGCTGTGGGAGAAAAAGAAGAAACAGATGGAAGAACGGATAATCCCGAATTGGACAATCCGGAGGAGGAACTTGTTTTGGTGAAAGAAGTAGAAAATGTCCCTGCTGAATCTCAAGGAGTACAGGAGATTCCAAAGACTTTGGACAATGATGTAGGAAGTGCTCCAGAAGTCAAAGAGGTGAAAGAGGAAAAACCCAGATTGGATCTTCGGAAGGTACCTCAGAAGGTTACTCCAATGAGCAAGGAGAGAAATGTGGCAAGTGTTAAGAAGAAGGCAGTTCCGCCAATAGCAAAAACACCAGAGAAATCCGCCCCTAGGGTGTCAAAGTCTATATCAACTCCCACCCCTAAAGCATCGAAGTCTACATCAACTCCCACCCCTAGAGCATCGAAGCCGCTATCAACTTCCACCCCTAGAGCATCGAAGCCGTTATCAACTTCCACCCCTAGAGTATCGAAGTCTATATCAACTTCCACACCTAGAGTGGCTAAGTCTATATCAACTTCCACCCCGAGAGTGGCTAAGTTTCTATCAACTTCTACTGCAACATCTTCATCGTGGTCCTCGGTAAAGAAGGGCAATGCTTCATCTCTCCCTAGGAGCAAAAATCCTTCAACTGAGGAAACCAAGAAAGCTCCTTCCAAGTCTTTGCACATGTCACTCAATTTGGAATCCAGTCCCACAATGTCAACTTCTGTGACCACTACAACCAGAAAGTCTTTCATTATGGAGAATATGGGAGATAAGGATATCGTCAAACGAGCATTTAAGTCGTTTCAAAACAATTACAACCAACCGAAACCCTCCATTGAAGAGAAACCTCCAACACCAAAGCAGCTCTCTACGAAGGGGAAAGAATCAAGGGTTTCCTCCTCTGTACCCCTGACAAAGGAGAATGGAGGGTATCTAGAAAAAAGAACTGCTAAGGCCGCTCCATCATCTGTTAGCTTGAGAAGTGATGAAAGCACAGATAAAAGAGAGTTTTCACAGAAATTGGCGGCAAAACCCAATGTGAAAGTGACAGAGAGAGCAAACTCCCAACCAAAGTCAAAGGATCAAAAGGAGGCAGAGATAAAAAAGCTCAGACAGAGTCTTAATTTTAAGGCTACTCCCACACCAGGCGTTTATCGGGGGCCAAAACTGTCAAAAAGTACTTCAGAAAAGGAGAGTTCCAACAATGAAACCCATCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

568

Amino Acids

62.4

Weight (kDa)

9.16

Isoelectric Point (pI)

41.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 486 - 554 3e-12 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 724
AccB1I GGYRCC 1 cut(s) 724
AccBSI CCGCTC 2 cut(s) 342, 1442
AccI GTMKAC 2 cut(s) 152, 872
AccIII TCCGGA 1 cut(s) 568
AciI CCGC 6 cut(s) 342, 791, 819, 905, 1440, 1508
AclWI GGATC 2 cut(s) 720, 1572
AcsI RAATTY 2 cut(s) 70, 427
AcuI CTGAAG 1 cut(s) 1650
AfaI GTAC 5 cut(s) 173, 630, 726, 1389, 1663
AfiI CCNNNNNNNGG 7 cut(s) 88, 222, 223, 825, 1027, 1399, 1628
AflIII ACRYGT 1 cut(s) 1164
AgsI TTSAA 6 cut(s) 69, 78, 236, 1125, 1289, 1322
AjnI CCWGG 4 cut(s) 26, 137, 202, 1627
AjuI GAANNNNNNNTTGG 2 cut(s) 158, 190
AloI GAACNNNNNNTCC 2 cut(s) 535, 567
AluBI AGCT 6 cut(s) 65, 512, 1145, 1348, 1458, 1591
AluI AGCT 6 cut(s) 65, 512, 1145, 1348, 1458, 1591
Alw21I GWGCWC 1 cut(s) 673
Alw26I GTCTC 1 cut(s) 50
AlwI GGATC 2 cut(s) 720, 1572
Ama87I CYCGRG 1 cut(s) 1021
Aor13HI TCCGGA 1 cut(s) 568
AoxI GGCC 3 cut(s) 213, 1437, 1643
ApeKI GCWGC 2 cut(s) 257, 1345
ApoI RAATTY 2 cut(s) 70, 427
Asp718I GGTACC 1 cut(s) 724
AspA2I CCTAGG 2 cut(s) 824, 1106
AspS9I GGNCC 3 cut(s) 214, 1071, 1643
AsuHPI GGTGA 4 cut(s) 17, 320, 601, 700
AsuII TTCGAA 1 cut(s) 372
AvaI CYCGRG 1 cut(s) 1021
AvaII GGWCC 1 cut(s) 1071
AvrII CCTAGG 2 cut(s) 824, 1106
BanI GGYRCC 1 cut(s) 724
Bbv12I GWGCWC 1 cut(s) 673
BbvI GCAGC 2 cut(s) 244, 1357
BccI CCATC 4 cut(s) 274, 346, 530, 1453
BceAI ACGGC 1 cut(s) 922
BcgI CGANNNNNNTGC 2 cut(s) 1263, 1297
BciT130I CCWGG 4 cut(s) 28, 139, 204, 1629
BclI TGATCA 1 cut(s) 331
BcoDI GTCTC 1 cut(s) 50
BfaI CTAG 7 cut(s) 825, 891, 924, 957, 990, 1107, 1418
BisI GCNGC 5 cut(s) 258, 905, 1346, 1440, 1509
BlnI CCTAGG 2 cut(s) 824, 1106
BlsI GCNGC 5 cut(s) 259, 906, 1347, 1441, 1510
BmcAI AGTACT 2 cut(s) 173, 1663
Bme1390I CCNGG 4 cut(s) 28, 139, 204, 1629
Bme18I GGWCC 1 cut(s) 1071
BmeT110I CYCGRG 1 cut(s) 1021
BmgT120I GGNCC 3 cut(s) 214, 1071, 1643
BmiI GGNNCC 2 cut(s) 726, 1644
BmrFI CCNGG 4 cut(s) 28, 139, 204, 1629
BmsI GCATC 4 cut(s) 22, 872, 905, 938
BpmI CTGGAG 1 cut(s) 657
Bpu10I CCTNAGC 1 cut(s) 1434
Bpu14I TTCGAA 1 cut(s) 372
BpuEI CTTGAG 3 cut(s) 452, 606, 1480
BsaJI CCNNGG 8 cut(s) 27, 138, 270, 360, 406, 824, 1074, 1106
BsaWI WCCGGW 1 cut(s) 568
BsaXI ACNNNNNCTCC 4 cut(s) 134, 164, 865, 895
Bsc4I CCNNNNNNNGG 7 cut(s) 88, 222, 223, 825, 1027, 1399, 1628
Bse1I ACTGG 1 cut(s) 1185
Bse3DI GCAATG 1 cut(s) 1096
BseAI TCCGGA 1 cut(s) 568
BseBI CCWGG 4 cut(s) 28, 139, 204, 1629
BseDI CCNNGG 8 cut(s) 27, 138, 270, 360, 406, 824, 1074, 1106
BseGI GGATG 3 cut(s) 37, 466, 481
BseLI CCNNNNNNNGG 7 cut(s) 88, 222, 223, 825, 1027, 1399, 1628
BseMI GCAATG 1 cut(s) 1096
BseMII CTCAG 3 cut(s) 743, 1119, 1606
BseNI ACTGG 1 cut(s) 1185
BseRI GAGGAG 3 cut(s) 287, 587, 1372
BseXI GCAGC 2 cut(s) 244, 1357
BshFI GGCC 3 cut(s) 215, 1439, 1645
BshNI GGYRCC 1 cut(s) 724
BsiHKAI GWGCWC 1 cut(s) 673
BsiHKCI CYCGRG 1 cut(s) 1021
BsiSI CCGG 1 cut(s) 569
BslFI GGGAC 2 cut(s) 593, 1173
BslI CCNNNNNNNGG 7 cut(s) 88, 222, 223, 825, 1027, 1399, 1628
BsmAI GTCTC 1 cut(s) 50
BsmFI GGGAC 2 cut(s) 593, 1173
BsnI GGCC 3 cut(s) 215, 1439, 1645
BsoBI CYCGRG 1 cut(s) 1021
Bsp119I TTCGAA 1 cut(s) 372
Bsp1286I GDGCHC 1 cut(s) 673
Bsp13I TCCGGA 1 cut(s) 568
Bsp143I GATC 6 cut(s) 331, 346, 415, 463, 712, 1564
BspACI CCGC 6 cut(s) 342, 791, 819, 905, 1440, 1508
BspANI GGCC 3 cut(s) 215, 1439, 1645
BspCNI CTCAG 3 cut(s) 742, 1120, 1605
BspEI TCCGGA 1 cut(s) 568
BspLI GGNNCC 2 cut(s) 726, 1644
BspPI GGATC 2 cut(s) 720, 1572
BspQI GCTCTTC 1 cut(s) 332
BspT104I TTCGAA 1 cut(s) 372
BspT107I GGYRCC 1 cut(s) 724
BsrBI CCGCTC 2 cut(s) 342, 1442
BsrDI GCAATG 1 cut(s) 1096
BsrI ACTGG 1 cut(s) 1185
BssECI CCNNGG 8 cut(s) 27, 138, 270, 360, 406, 824, 1074, 1106
BssMI GATC 6 cut(s) 331, 346, 415, 463, 712, 1564
BssT1I CCWWGG 3 cut(s) 406, 824, 1106
Bst2UI CCWGG 4 cut(s) 28, 139, 204, 1629
Bst4CI ACNGT 3 cut(s) 135, 392, 1653
Bst6I CTCTTC 2 cut(s) 332, 1317
BstBI TTCGAA 1 cut(s) 372
BstDEI CTNAG 6 cut(s) 729, 999, 1032, 1128, 1434, 1592
BstENI CCTNNNNNAGG 1 cut(s) 1397
BstF5I GGATG 3 cut(s) 37, 466, 481
BstKTI GATC 6 cut(s) 334, 349, 418, 466, 715, 1567
BstMAI GTCTC 1 cut(s) 50
BstMBI GATC 6 cut(s) 331, 346, 415, 463, 712, 1564
BstMWI GCNNNNNNNGC 4 cut(s) 32, 266, 292, 1439
BstNI CCWGG 4 cut(s) 28, 139, 204, 1629
BstNSI RCATGY 1 cut(s) 1168
BstSCI CCNGG 4 cut(s) 26, 137, 202, 1627
BstV1I GCAGC 2 cut(s) 244, 1357
BstX2I RGATCY 1 cut(s) 712
BstYI RGATCY 1 cut(s) 712
BsuRI GGCC 3 cut(s) 215, 1439, 1645
BtsCI GGATG 3 cut(s) 37, 466, 481
BtsIMutI CAGTG 1 cut(s) 131
Cfr13I GGNCC 3 cut(s) 214, 1071, 1643
Csp6I GTAC 5 cut(s) 172, 629, 725, 1388, 1662
CviAII CATG 2 cut(s) 455, 1165
CviQI GTAC 5 cut(s) 172, 629, 725, 1388, 1662
DdeI CTNAG 6 cut(s) 729, 999, 1032, 1128, 1434, 1592
DpnI GATC 6 cut(s) 333, 348, 417, 465, 714, 1566
DpnII GATC 6 cut(s) 331, 346, 415, 463, 712, 1564
Eam1104I CTCTTC 2 cut(s) 332, 1317
EarI CTCTTC 2 cut(s) 332, 1317
EciI GGCGGA 2 cut(s) 780, 808
Eco130I CCWWGG 3 cut(s) 406, 824, 1106
Eco32I GATATC 1 cut(s) 1261
Eco47I GGWCC 1 cut(s) 1071
Eco57I CTGAAG 1 cut(s) 1650
Eco88I CYCGRG 1 cut(s) 1021
EcoNI CCTNNNNNAGG 1 cut(s) 1397
EcoRII CCWGG 4 cut(s) 26, 137, 202, 1627
EcoRV GATATC 1 cut(s) 1261
EcoT14I CCWWGG 3 cut(s) 406, 824, 1106
ErhI CCWWGG 3 cut(s) 406, 824, 1106
FaeI CATG 2 cut(s) 458, 1168
FaiI YATR 8 cut(s) 456, 491, 842, 974, 1007, 1166, 1238, 1247
FaqI GGGAC 2 cut(s) 593, 1173
FatI CATG 2 cut(s) 454, 1164
FbaI TGATCA 1 cut(s) 331
FblI GTMKAC 2 cut(s) 152, 872
Fnu4HI GCNGC 5 cut(s) 258, 905, 1346, 1440, 1509
FokI GGATG 3 cut(s) 44, 473, 488
Fsp4HI GCNGC 5 cut(s) 258, 905, 1346, 1440, 1509
FspBI CTAG 7 cut(s) 825, 891, 924, 957, 990, 1107, 1418
GluI GCNGC 5 cut(s) 258, 905, 1346, 1440, 1509
GsuI CTGGAG 1 cut(s) 657
HaeIII GGCC 3 cut(s) 215, 1439, 1645
HapII CCGG 1 cut(s) 569
Hin1II CATG 2 cut(s) 458, 1168
HincII GTYRAC 2 cut(s) 153, 1200
HindII GTYRAC 2 cut(s) 153, 1200
HinfI GANTC 9 cut(s) 8, 125, 326, 356, 617, 637, 1181, 1367, 1600
HpaII CCGG 1 cut(s) 569
HphI GGTGA 4 cut(s) 17, 320, 601, 700
Hpy166II GTNNAC 3 cut(s) 153, 873, 1200
Hpy188III TCNNGA 6 cut(s) 89, 419, 554, 569, 674, 1418
Hpy8I GTNNAC 3 cut(s) 153, 873, 1200
HpyAV CCTTC 7 cut(s) 715, 727, 775, 1078, 1131, 1158, 1351
HpyCH4III ACNGT 3 cut(s) 135, 392, 1653
HpyCH4V TGCA 3 cut(s) 301, 1055, 1162
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 266, 292, 1439
HpyF3I CTNAG 6 cut(s) 729, 999, 1032, 1128, 1434, 1592
Hsp92II CATG 2 cut(s) 458, 1168
Kpn2I TCCGGA 1 cut(s) 568
KpnI GGTACC 1 cut(s) 728
Ksp22I TGATCA 1 cut(s) 331
Kzo9I GATC 6 cut(s) 331, 346, 415, 463, 712, 1564
LguI GCTCTTC 1 cut(s) 332
LmnI GCTCC 5 cut(s) 283, 676, 1110, 1150, 1447
Lsp1109I GCAGC 2 cut(s) 244, 1357
LweI GCATC 4 cut(s) 22, 872, 905, 938
MaeI CTAG 7 cut(s) 825, 891, 924, 957, 990, 1107, 1418
MaeIII GTNAC 4 cut(s) 736, 1167, 1207, 1528
MalI GATC 6 cut(s) 333, 348, 417, 465, 714, 1566
MbiI CCGCTC 2 cut(s) 342, 1442
MboI GATC 6 cut(s) 331, 346, 415, 463, 712, 1564
MflI RGATCY 1 cut(s) 712
MhlI GDGCHC 1 cut(s) 673
MluCI AATT 8 cut(s) 70, 375, 427, 557, 1174, 1294, 1502, 1606
MlyI GAGTC 1 cut(s) 1609
MmeI TCCRAC 2 cut(s) 303, 1358
MroI TCCGGA 1 cut(s) 568
MseI TTAA 4 cut(s) 774, 1278, 1605, 1611
MslI CAYNNNNRTG 1 cut(s) 459
MspI CCGG 1 cut(s) 569
MspR9I CCNGG 4 cut(s) 28, 139, 204, 1629
MvaI CCWGG 4 cut(s) 28, 139, 204, 1629
MwoI GCNNNNNNNGC 4 cut(s) 32, 266, 292, 1439
NdeII GATC 6 cut(s) 331, 346, 415, 463, 712, 1564
NlaIII CATG 2 cut(s) 458, 1168
NlaIV GGNNCC 2 cut(s) 726, 1644
NmeAIII GCCGAG 1 cut(s) 295
NmuCI GTSAC 3 cut(s) 1167, 1207, 1528
NspI RCATGY 1 cut(s) 1168
NspV TTCGAA 1 cut(s) 372
PciI ACATGT 1 cut(s) 1164
PciSI GCTCTTC 1 cut(s) 332
PfeI GAWTC 8 cut(s) 8, 125, 326, 356, 617, 637, 1181, 1367
PflFI GACNNNGTC 1 cut(s) 1599
PkrI GCNGC 5 cut(s) 259, 906, 1347, 1441, 1510
PleI GAGTC 1 cut(s) 1608
PpsI GAGTC 1 cut(s) 1608
PscI ACATGT 1 cut(s) 1164
Psp6I CCWGG 4 cut(s) 26, 137, 202, 1627
PspGI CCWGG 4 cut(s) 26, 137, 202, 1627
PspN4I GGNNCC 2 cut(s) 726, 1644
PspPI GGNCC 3 cut(s) 214, 1071, 1643
PsuI RGATCY 1 cut(s) 712
PsyI GACNNNGTC 1 cut(s) 1599
RsaI GTAC 5 cut(s) 173, 630, 726, 1389, 1663
RsaNI GTAC 5 cut(s) 172, 629, 725, 1388, 1662
RseI CAYNNNNRTG 1 cut(s) 459
SalI GTCGAC 1 cut(s) 151
SapI GCTCTTC 1 cut(s) 332
SaqAI TTAA 4 cut(s) 774, 1278, 1605, 1611
SatI GCNGC 5 cut(s) 258, 905, 1346, 1440, 1509
Sau3AI GATC 6 cut(s) 331, 346, 415, 463, 712, 1564
Sau96I GGNCC 3 cut(s) 214, 1071, 1643
ScaI AGTACT 2 cut(s) 173, 1663
SchI GAGTC 1 cut(s) 1609
ScrFI CCNGG 4 cut(s) 28, 139, 204, 1629
SduI GDGCHC 1 cut(s) 673
SfaNI GCATC 4 cut(s) 22, 872, 905, 938
SfuI TTCGAA 1 cut(s) 372
SinI GGWCC 1 cut(s) 1071
SmiMI CAYNNNNRTG 1 cut(s) 459
SmlI CTYRAG 3 cut(s) 467, 621, 1459
SmoI CTYRAG 3 cut(s) 467, 621, 1459
Sse9I AATT 8 cut(s) 70, 375, 427, 557, 1174, 1294, 1502, 1606
SsiI CCGC 6 cut(s) 342, 791, 819, 905, 1440, 1508
SspMI CTAG 7 cut(s) 825, 891, 924, 957, 990, 1107, 1418
StyD4I CCNGG 4 cut(s) 26, 137, 202, 1627
StyI CCWWGG 3 cut(s) 406, 824, 1106
TaaI ACNGT 3 cut(s) 135, 392, 1653
TasI AATT 8 cut(s) 70, 375, 427, 557, 1174, 1294, 1502, 1606
TatI WGTACW 3 cut(s) 171, 628, 1661
TauI GCSGC 3 cut(s) 907, 1442, 1511
TfiI GAWTC 8 cut(s) 8, 125, 326, 356, 617, 637, 1181, 1367
Tru1I TTAA 4 cut(s) 774, 1278, 1605, 1611
Tru9I TTAA 4 cut(s) 774, 1278, 1605, 1611
TscAI CASTG 1 cut(s) 138
TseFI GTSAC 3 cut(s) 1167, 1207, 1528
TseI GCWGC 2 cut(s) 257, 1345
Tsp45I GTSAC 3 cut(s) 1167, 1207, 1528
TspDTI ATGAA 5 cut(s) 1053, 1086, 1222, 1485, 1704
TspGWI ACGGA 1 cut(s) 560
TspRI CASTG 1 cut(s) 138
Tth111I GACNNNGTC 1 cut(s) 1599
VpaK11BI GGWCC 1 cut(s) 1071
XagI CCTNNNNNAGG 1 cut(s) 1397
XapI RAATTY 2 cut(s) 70, 427
XbaI TCTAGA 1 cut(s) 1417
XceI RCATGY 1 cut(s) 1168
XcmI CCANNNNNNNNNTGG 2 cut(s) 991, 1024
XmaJI CCTAGG 2 cut(s) 824, 1106
XmiI GTMKAC 2 cut(s) 152, 872
XspI CTAG 7 cut(s) 825, 891, 924, 957, 990, 1107, 1418
ZrmI AGTACT 2 cut(s) 173, 1663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.