Rw4G035750

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0003792
Physical Location & Seq
Reverse (-)
62588267 .. 62591024
2758 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G035750.1

Sequence Viewer

Length: 1716 bp
ATGGGTGAATCAATAGTGGGTTTTCCAAAAGATGCTGATAAGATGGGAGAGATAGCTGGTACAGATTCATCAAATCCTTCTCTGGAAGTGTCGGTTTCATTTGGTAGGTTTGAGAATGATTCATTATCTTGGGAGAAATGGTCGGCATTTTCGCCAAATAAGTACTTGGAAGAAGTTGAGAAGTGTGCTACTCCTGGTTCGGTAGCTCAGAAAAGGGCTTATTTTGAAGCTCATTACAAGAGGATAGCTGCTCGAAAAGCCGAGGAGCTTCTGGAACAGGAGAAGCAAATGCAAGATGATGATGATCCCTTAAAGTCAGATGATCAAAATAATGGAGATCAGATCTGTTGTGGTACAGATTGTGGAATTGATGTAGCTACCAGTCAAACTAGTGCTCAAGCAAATAGTCAAGAGCCCAATATAGAGAATGGGATGAGCAGTACTCATGTGGAAGATCTCAAGGAGGAGGATGAGGATGTTTTCACTATGGAATGTCAAACATCATCGATTGAGGGTAGAGAAATAGAGGAAACTGATAGCGGAGTGGTTAGTCCGAAGACTCCAAAGCTGAACAGACCAGAAGAACTTGTTTTGGTAAAAGAAGTGGAAACCATCACTGCTGACACTCAAGAAACAATACAGGAAGTTCCAAAGACTTTGGACAGTGATACAGGCAATGCTCCAGAAGTTAAAGAGGAAAAAGCGAGATTCGATCTTCAGAAGAGATCTCAGAAGGTTACTCCAGTGAACAAGGAGAGGACAGTGGCAAAGGCCAAGAAGAAATCAATTTCACCAATGACTAAAACACCTCAGAATCCGACCCCTAGACTGTCAAAGTTACCACAGAATTCCACCCCTAGAGTGTCAAAGATACCGCAGAATACTACCCCTAGAGTGTCAAAGATACTGCAGAATACCACCCCTAGTGTGTCAAAGCCAATATCAGCTCCTACCGGAGCAAAATCTGCACCAAGATCCTCAGTAACGAAGGCCAATGGTTCATCTTTAACTAGGAGCACAAATCAGTCAAGTGAAAAAACCAAGAAAGTGCCTCCTAAATCTCTGCACATGTCACTCAGTTTCAATCCTAAAAAGTCTGATTCAGCTTCAGAAACTGTGGTTACAGCTAGAAAATCTTTGATTATGGAGCAAATGGGTGACAAGGACATTGTCAAACGAGCATTCAAGACATTTCAAAACAGTGTCAACCAATTGAAATCCTCTAATGAAGAGAAACCTTCAGCACCAATGCAGCTCCCTACAAAGTCGAAAGAACCAAAGGTTTCCACTCCTATGCCTCTGCCGAAAGACACTGGAGGGTCACTCAAAACGAGTTATCCGGATAAAAGAAATGCCAAGGCTGCCCCATCATCTTTAGGCCTGAGAAGTGAAGAAAGAGCAGAGAAACGGAAGGAGTTAACAAACAACTTGGCGAAACCCAATGCCAGAGATGCAGAGAGAGCACACTTCCAGCGAAAGTCAAAGGAGCAAAAGGAGGCAGAGATAAAAAAGCTAAGACAGAGTCTTAAGTTCAAGGCTACACCAACGCCTGATTCTTATCGGGGACAAAAGTTATCGAAAAGTACTCTAGAAAAGAAGCTAGAGAAGAATGAGACACATATGAAGGAATATCCTTCTCATGAATGGTCCATGCTTGGATATTACTGCTTAGCTGTTGATCATAATGAGTCACAATCTGTATATCTGGATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

571

Amino Acids

63.42

Weight (kDa)

8.65

Isoelectric Point (pI)

47.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 461 - 528 1.2e-12 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 1339
AciI CCGC 2 cut(s) 540, 875
AclWI GGATC 2 cut(s) 299, 969
AcsI RAATTY 1 cut(s) 847
AcuI CTGAAG 3 cut(s) 701, 1092, 1224
AfaI GTAC 5 cut(s) 61, 164, 355, 442, 1585
AflII CTTAAG 1 cut(s) 1526
AflIII ACRYGT 1 cut(s) 1068
AgsI TTSAA 6 cut(s) 227, 1084, 1186, 1196, 1216, 1534
AhlI ACTAGT 1 cut(s) 389
AjnI CCWGG 1 cut(s) 193
AjuI GAANNNNNNNTTGG 2 cut(s) 1537, 1569
Alw21I GWGCWC 3 cut(s) 397, 1019, 1465
Alw26I GTCTC 1 cut(s) 1607
AlwI GGATC 2 cut(s) 299, 969
AlwNI CAGNNNCTG 1 cut(s) 1115
Aor13HI TCCGGA 1 cut(s) 1339
AoxI GGCC 3 cut(s) 771, 990, 1378
ApeKI GCWGC 3 cut(s) 248, 1252, 1361
ApoI RAATTY 1 cut(s) 847
AspS9I GGNCC 1 cut(s) 1647
AsuHPI GGTGA 3 cut(s) 17, 783, 1169
AvaII GGWCC 1 cut(s) 1647
BanII GRGCYC 1 cut(s) 417
BbsI GAAGAC 1 cut(s) 563
Bbv12I GWGCWC 3 cut(s) 397, 1019, 1465
BbvI GCAGC 3 cut(s) 235, 1264, 1348
BccI CCATC 3 cut(s) 37, 620, 1375
BciT130I CCWGG 1 cut(s) 195
BclI TGATCA 2 cut(s) 322, 1678
BcoDI GTCTC 1 cut(s) 1607
BcuI ACTAGT 1 cut(s) 389
BfaI CTAG 9 cut(s) 390, 825, 858, 891, 924, 1011, 1128, 1589, 1601
BfmI CTRYAG 1 cut(s) 908
BfrI CTTAAG 1 cut(s) 1526
BglII AGATCT 3 cut(s) 342, 454, 725
BisI GCNGC 3 cut(s) 249, 1253, 1362
BlpI GCTNAGC 1 cut(s) 1669
BlsI GCNGC 3 cut(s) 250, 1254, 1363
BmcAI AGTACT 3 cut(s) 164, 442, 1585
Bme1390I CCNGG 1 cut(s) 195
Bme18I GGWCC 1 cut(s) 1647
BmgT120I GGNCC 1 cut(s) 1647
BmrFI CCNGG 1 cut(s) 195
BmsI GCATC 2 cut(s) 22, 1441
BpiI GAAGAC 1 cut(s) 563
BplI GAGNNNNNCTC 4 cut(s) 427, 459, 1308, 1340
BpmI CTGGAG 3 cut(s) 666, 726, 1335
Bpu1102I GCTNAGC 1 cut(s) 1669
BpuEI CTTGAG 3 cut(s) 381, 443, 612
Bsa29I ATCGAT 1 cut(s) 506
BsaBI GATNNNNATC 2 cut(s) 303, 1557
BsaJI CCNNGG 2 cut(s) 261, 1356
BsaWI WCCGGW 2 cut(s) 953, 1339
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bse1I ACTGG 3 cut(s) 381, 743, 1318
Bse3DI GCAATG 1 cut(s) 682
Bse8I GATNNNNATC 2 cut(s) 303, 1557
BseAI TCCGGA 1 cut(s) 1339
BseBI CCWGG 1 cut(s) 195
BseCI ATCGAT 1 cut(s) 506
BseDI CCNNGG 2 cut(s) 261, 1356
BseGI GGATG 4 cut(s) 438, 475, 481, 1714
BseJI GATNNNNATC 2 cut(s) 303, 1557
BseMI GCAATG 1 cut(s) 682
BseMII CTCAG 6 cut(s) 221, 743, 824, 993, 1090, 1373
BseNI ACTGG 3 cut(s) 381, 743, 1318
BseRI GAGGAG 2 cut(s) 278, 479
BseXI GCAGC 3 cut(s) 235, 1264, 1348
BsgI GTGCAG 2 cut(s) 951, 1049
BshFI GGCC 3 cut(s) 773, 992, 1380
BshVI ATCGAT 1 cut(s) 506
BsiHKAI GWGCWC 3 cut(s) 397, 1019, 1465
BsiSI CCGG 2 cut(s) 954, 1340
BslFI GGGAC 1 cut(s) 1578
BsmAI GTCTC 1 cut(s) 1607
BsmFI GGGAC 1 cut(s) 1578
BsmI GAATGC 1 cut(s) 1181
BsnI GGCC 3 cut(s) 773, 992, 1380
Bsp1286I GDGCHC 4 cut(s) 397, 417, 1019, 1465
Bsp13I TCCGGA 1 cut(s) 1339
Bsp143I GATC 9 cut(s) 304, 322, 337, 342, 454, 712, 725, 974, 1678
Bsp1720I GCTNAGC 1 cut(s) 1669
BspACI CCGC 2 cut(s) 540, 875
BspANI GGCC 3 cut(s) 773, 992, 1380
BspCNI CTCAG 6 cut(s) 220, 742, 823, 992, 1089, 1374
BspDI ATCGAT 1 cut(s) 506
BspEI TCCGGA 1 cut(s) 1339
BspHI TCATGA 1 cut(s) 1639
BspMAI CTGCAG 1 cut(s) 912
BspPI GGATC 2 cut(s) 299, 969
BspTI CTTAAG 1 cut(s) 1526
BsrDI GCAATG 1 cut(s) 682
BsrI ACTGG 3 cut(s) 381, 743, 1318
BssECI CCNNGG 2 cut(s) 261, 1356
BssMI GATC 9 cut(s) 304, 322, 337, 342, 454, 712, 725, 974, 1678
BssT1I CCWWGG 1 cut(s) 1356
Bst2UI CCWGG 1 cut(s) 195
Bst4CI ACNGT 5 cut(s) 665, 763, 831, 1117, 1202
Bst6I CTCTTC 2 cut(s) 716, 1224
BstAFI CTTAAG 1 cut(s) 1526
BstAPI GCANNNNNTGC 1 cut(s) 965
BstDEI CTNAG 8 cut(s) 207, 729, 810, 979, 1076, 1382, 1514, 1669
BstF5I GGATG 4 cut(s) 438, 475, 481, 1714
BstKTI GATC 9 cut(s) 307, 325, 340, 345, 457, 715, 728, 977, 1681
BstMAI GTCTC 1 cut(s) 1607
BstMBI GATC 9 cut(s) 304, 322, 337, 342, 454, 712, 725, 974, 1678
BstMWI GCNNNNNNNGC 5 cut(s) 257, 965, 1361, 1451, 1460
BstNI CCWGG 1 cut(s) 195
BstNSI RCATGY 1 cut(s) 1072
BstSCI CCNGG 1 cut(s) 193
BstSFI CTRYAG 1 cut(s) 908
BstV1I GCAGC 3 cut(s) 235, 1264, 1348
BstV2I GAAGAC 1 cut(s) 563
BstX2I RGATCY 4 cut(s) 342, 454, 725, 974
BstYI RGATCY 4 cut(s) 342, 454, 725, 974
Bsu15I ATCGAT 1 cut(s) 506
BsuRI GGCC 3 cut(s) 773, 992, 1380
BsuTUI ATCGAT 1 cut(s) 506
BtsCI GGATG 4 cut(s) 438, 475, 481, 1714
BtsI GCAGTG 1 cut(s) 615
BtsIMutI CAGTG 6 cut(s) 615, 670, 750, 768, 1207, 1311
CaiI CAGNNNCTG 1 cut(s) 1115
CciI TCATGA 1 cut(s) 1639
Cfr13I GGNCC 1 cut(s) 1647
ClaI ATCGAT 1 cut(s) 506
Csp6I GTAC 5 cut(s) 60, 163, 354, 441, 1584
CviAII CATG 4 cut(s) 446, 1069, 1640, 1651
CviQI GTAC 5 cut(s) 60, 163, 354, 441, 1584
DdeI CTNAG 8 cut(s) 207, 729, 810, 979, 1076, 1382, 1514, 1669
DpnI GATC 9 cut(s) 306, 324, 339, 344, 456, 714, 727, 976, 1680
DpnII GATC 9 cut(s) 304, 322, 337, 342, 454, 712, 725, 974, 1678
Eam1104I CTCTTC 2 cut(s) 716, 1224
EarI CTCTTC 2 cut(s) 716, 1224
Eco130I CCWWGG 1 cut(s) 1356
Eco147I AGGCCT 1 cut(s) 1380
Eco24I GRGCYC 1 cut(s) 417
Eco47I GGWCC 1 cut(s) 1647
Eco57I CTGAAG 3 cut(s) 701, 1092, 1224
EcoRI GAATTC 1 cut(s) 847
EcoRII CCWGG 1 cut(s) 193
EcoT14I CCWWGG 1 cut(s) 1356
EcoT38I GRGCYC 1 cut(s) 417
ErhI CCWWGG 1 cut(s) 1356
FaeI CATG 4 cut(s) 449, 1072, 1643, 1654
FaqI GGGAC 1 cut(s) 1578
FatI CATG 4 cut(s) 445, 1068, 1639, 1650
FauNDI CATATG 1 cut(s) 1620
FbaI TGATCA 2 cut(s) 322, 1678
Fnu4HI GCNGC 3 cut(s) 249, 1253, 1362
FokI GGATG 3 cut(s) 445, 482, 488
FriOI GRGCYC 1 cut(s) 417
Fsp4HI GCNGC 3 cut(s) 249, 1253, 1362
FspBI CTAG 9 cut(s) 390, 825, 858, 891, 924, 1011, 1128, 1589, 1601
GluI GCNGC 3 cut(s) 249, 1253, 1362
GsuI CTGGAG 3 cut(s) 666, 726, 1335
HaeIII GGCC 3 cut(s) 773, 992, 1380
HapII CCGG 2 cut(s) 954, 1340
Hin1II CATG 4 cut(s) 449, 1072, 1643, 1654
HincII GTYRAC 2 cut(s) 1207, 1419
HindII GTYRAC 2 cut(s) 1207, 1419
HpaI GTTAAC 1 cut(s) 1419
HpaII CCGG 2 cut(s) 954, 1340
HphI GGTGA 3 cut(s) 17, 783, 1169
Hpy166II GTNNAC 3 cut(s) 748, 1207, 1419
Hpy8I GTNNAC 3 cut(s) 748, 1207, 1419
HpyAV CCTTC 7 cut(s) 87, 727, 982, 1248, 1405, 1618, 1644
HpyCH4III ACNGT 5 cut(s) 665, 763, 831, 1117, 1202
HpyCH4V TGCA 6 cut(s) 292, 910, 968, 1066, 1252, 1454
HpyF10VI GCNNNNNNNGC 5 cut(s) 257, 965, 1361, 1451, 1460
HpyF3I CTNAG 8 cut(s) 207, 729, 810, 979, 1076, 1382, 1514, 1669
Hsp92II CATG 4 cut(s) 449, 1072, 1643, 1654
Kpn2I TCCGGA 1 cut(s) 1339
Ksp22I TGATCA 2 cut(s) 322, 1678
KspAI GTTAAC 1 cut(s) 1419
Kzo9I GATC 9 cut(s) 304, 322, 337, 342, 454, 712, 725, 974, 1678
LmnI GCTCC 8 cut(s) 265, 685, 952, 956, 1014, 1147, 1260, 1486
Lsp1109I GCAGC 3 cut(s) 235, 1264, 1348
LweI GCATC 2 cut(s) 22, 1441
MaeI CTAG 9 cut(s) 390, 825, 858, 891, 924, 1011, 1128, 1589, 1601
MaeIII GTNAC 8 cut(s) 736, 837, 982, 1071, 1120, 1157, 1320, 1689
MalI GATC 9 cut(s) 306, 324, 339, 344, 456, 714, 727, 976, 1680
MboI GATC 9 cut(s) 304, 322, 337, 342, 454, 712, 725, 974, 1678
MfeI CAATTG 1 cut(s) 1211
MflI RGATCY 4 cut(s) 342, 454, 725, 974
MhlI GDGCHC 4 cut(s) 397, 417, 1019, 1465
MluCI AATT 4 cut(s) 366, 786, 847, 1211
MlyI GAGTC 3 cut(s) 553, 1531, 1697
MmeI TCCRAC 1 cut(s) 842
MroI TCCGGA 1 cut(s) 1339
MseI TTAA 5 cut(s) 311, 690, 1007, 1418, 1527
MslI CAYNNNNRTG 1 cut(s) 1292
MspCI CTTAAG 1 cut(s) 1526
MspI CCGG 2 cut(s) 954, 1340
MspR9I CCNGG 1 cut(s) 195
MunI CAATTG 1 cut(s) 1211
Mva1269I GAATGC 1 cut(s) 1181
MvaI CCWGG 1 cut(s) 195
MwoI GCNNNNNNNGC 5 cut(s) 257, 965, 1361, 1451, 1460
NdeI CATATG 1 cut(s) 1620
NdeII GATC 9 cut(s) 304, 322, 337, 342, 454, 712, 725, 974, 1678
NlaIII CATG 4 cut(s) 449, 1072, 1643, 1654
NmeAIII GCCGAG 1 cut(s) 286
NmuCI GTSAC 4 cut(s) 1071, 1157, 1320, 1689
NspI RCATGY 1 cut(s) 1072
PagI TCATGA 1 cut(s) 1639
PceI AGGCCT 1 cut(s) 1380
PciI ACATGT 1 cut(s) 1068
PctI GAATGC 1 cut(s) 1181
PfeI GAWTC 7 cut(s) 8, 65, 119, 708, 814, 1100, 1553
PflFI GACNNNGTC 2 cut(s) 1169, 1521
PkrI GCNGC 3 cut(s) 250, 1254, 1363
PleI GAGTC 3 cut(s) 553, 1530, 1696
PpsI GAGTC 3 cut(s) 553, 1530, 1696
PscI ACATGT 1 cut(s) 1068
Psp6I CCWGG 1 cut(s) 193
PspGI CCWGG 1 cut(s) 193
PspPI GGNCC 1 cut(s) 1647
PstI CTGCAG 1 cut(s) 912
PstNI CAGNNNCTG 1 cut(s) 1115
PsuI RGATCY 4 cut(s) 342, 454, 725, 974
PsyI GACNNNGTC 2 cut(s) 1169, 1521
RsaI GTAC 5 cut(s) 61, 164, 355, 442, 1585
RsaNI GTAC 5 cut(s) 60, 163, 354, 441, 1584
RseI CAYNNNNRTG 1 cut(s) 1292
SaqAI TTAA 5 cut(s) 311, 690, 1007, 1418, 1527
SatI GCNGC 3 cut(s) 249, 1253, 1362
Sau3AI GATC 9 cut(s) 304, 322, 337, 342, 454, 712, 725, 974, 1678
Sau96I GGNCC 1 cut(s) 1647
ScaI AGTACT 3 cut(s) 164, 442, 1585
SchI GAGTC 3 cut(s) 553, 1531, 1697
ScrFI CCNGG 1 cut(s) 195
SduI GDGCHC 4 cut(s) 397, 417, 1019, 1465
SfaNI GCATC 2 cut(s) 22, 1441
SfcI CTRYAG 1 cut(s) 908
SinI GGWCC 1 cut(s) 1647
SmiMI CAYNNNNRTG 1 cut(s) 1292
SmlI CTYRAG 4 cut(s) 396, 458, 627, 1526
SmoI CTYRAG 4 cut(s) 396, 458, 627, 1526
SpeI ACTAGT 1 cut(s) 389
Sse9I AATT 4 cut(s) 366, 786, 847, 1211
SseBI AGGCCT 1 cut(s) 1380
SsiI CCGC 2 cut(s) 540, 875
SspMI CTAG 9 cut(s) 390, 825, 858, 891, 924, 1011, 1128, 1589, 1601
StuI AGGCCT 1 cut(s) 1380
StyD4I CCNGG 1 cut(s) 193
StyI CCWWGG 1 cut(s) 1356
TaaI ACNGT 5 cut(s) 665, 763, 831, 1117, 1202
TaqI TCGA 5 cut(s) 253, 506, 711, 1268, 1577
TasI AATT 4 cut(s) 366, 786, 847, 1211
TatI WGTACW 3 cut(s) 162, 440, 1583
TfiI GAWTC 7 cut(s) 8, 65, 119, 708, 814, 1100, 1553
Tru1I TTAA 5 cut(s) 311, 690, 1007, 1418, 1527
Tru9I TTAA 5 cut(s) 311, 690, 1007, 1418, 1527
TscAI CASTG 6 cut(s) 622, 670, 750, 768, 1207, 1318
TseFI GTSAC 4 cut(s) 1071, 1157, 1320, 1689
TseI GCWGC 3 cut(s) 248, 1252, 1361
Tsp45I GTSAC 4 cut(s) 1071, 1157, 1320, 1689
TspDTI ATGAA 7 cut(s) 57, 87, 111, 990, 1242, 1637, 1656
TspGWI ACGGA 1 cut(s) 1423
TspRI CASTG 6 cut(s) 622, 670, 750, 768, 1207, 1318
Tth111I GACNNNGTC 2 cut(s) 1169, 1521
Vha464I CTTAAG 1 cut(s) 1526
VpaK11BI GGWCC 1 cut(s) 1647
XapI RAATTY 1 cut(s) 847
XbaI TCTAGA 1 cut(s) 1588
XceI RCATGY 1 cut(s) 1072
XspI CTAG 9 cut(s) 390, 825, 858, 891, 924, 1011, 1128, 1589, 1601
ZrmI AGTACT 3 cut(s) 164, 442, 1585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.