Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002819

Physical Location & Seq

Genomic Coordinates Reverse (-)

27936508 .. 27940369

Gene Structure 3862 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G74310.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2736 bp

ATGAATCCAGAGAAATTCACACACAAGACAAACGAGACAATTGCTACAGCTCATGAGCTAGCTGTGAATGCAGGACATGCTCAATTCACTCCTTTGCATTTAGCTGGTGCTTTGATCTCTGATCCCACCGGTATATTTCCTCAAGCAATCTCTAGTGCCGGTGGCGAGAACGCAGCTCAATCTGCTGAAAGAGTGATCAATCAAGCCTTGAAGAAGCTTCCTTCACAATCTCCTCCACCTGATGATATTCCAGCGAGTTCTAGTCTTATTAAGGTCATTCGTCGTGCTCAAGCTGCTCAGAAGTCACGAGGTGATACTCATTTGGCTGTTGACCAGTTGATTATGGGTCTTCTTGAAGATTCTCAAATCAGGGATTTGTTGAACGAAGTCGGTGTAGCGACGGCGAGGGTAAAGTCTGAGGTTGAGAAGCTTCGTGGGAAAGAAGGGAAGAAAGTTGAGAGTGCTTCAGGGGACACAAATTTTCAAGCTTTAAAGACTTATGGAAGAGATTTGGTTGAGCAAGCAGGGAAGCTTGATCCTGTGATTGGTCGTGATGAGGAGATTAGAAGAGTCGTGAGGATTCTTTCGAGGAGAACGAAGAACAATCCTGTGCTTATTGGAGAGCCAGGAGTTGGTAAAACAGCTGTGGTTGAAGGTTTAGCACAAAGGATTGTGAAAGGAGATGTGCCCAACAGTCTTACTGATGTGAGATTAATTTCGTTGGACATGGGTGCGTTAGTTGCTGGTGCTAAATACCGAGGAGAGTTTGAAGAAAGGTTGAAATCTGTTTTGAAAGAAGTTGAGGACGCTGAAGGCAAAGTGATTCTCTTTATTGATGAGATTCATTTGGTTCTTGGTGCTGGCAAAACTGAAGGGTCGATGGATGCAGCTAATCTGTTCAAGCCCATGTTAGCTAGAGGGCAGCTTCGATGCATTGGTGCTACAACGCTTGAAGAATACAGGAAATATGTTGAGAAAGATGCTGCCTTTGAGAGGAGGTTCCAACAAGTCTATGTTGCGGAGCCAAGTGTGCCTGACACCATTAGTATCCTTAGAGGACTCAAGGAGAAGTATGAGGGACATCATGGTGTGCGAATCCAAGACAGAGCTCTTATAAATGCTGCTCAGCTGTCTGCTCGTTACATAACTGGTCGGCATTTACCGGATAAAGCAATTGATTTGGTTGATGAGGCTTGTGCGAATGTGAGAGTCCAGCTTGATAGTCAACCTGAAGAGATTGATAACCTTGAAAGGAAGAGGATGCAGCTGGAAATTGAACTTCACGCCTTGGAAAGGGAGAAGGATAAAGCCAGCAAAGCTCGACTTATAGAGGTGCGGAAAGAGCTTGATGACCTGAGAGACAAGCTTCAGCCTCTCACGATGAAATACAGAAAGGAGAAAGAGAGAATTGATGAGATTCGAAGGCTTAAACAGAAAAGAGAAGAGCTCATGTTTTCTTTGCAGGAGGCAGAACGAAGATATGACCTTGCAAGAGCTGCTGATCTAAGATATGGCGCAATTCAAGAAGTGGAATCTGCAATTGCCCAACTTGAAGGAACTTCTTCTGAAGAGAATGTGATGCTCACAGAAAACGTTGGGCCTGAACACATTGCTGAGGTTGTGAGCCGTTGGACAGGGATTCCAGTGACGAGACTTGGCCAAAATGAGAAGGAGAGGTTGATTGGTCTTGCTGATAGGTTGCATAAGCGGGTTGTGGGACAGAATCAAGCGGTAAATGCAGTTTCTGAGGCAATTCTAAGGTCAAGGGCAGGACTTGGAAGGCCACAACAGCCAACTGGATCATTCTTATTCCTTGGACCAACTGGTGTTGGCAAAACTGAGCTCGCCAAGGCTCTTGCTGAGCAGCTGTTTGATGATGAAAACCTCTTAGTTCGGATTGATATGTCGGAATATATGGAACAACACTCTGTCTCTCGCCTCATTGGGGCACCACCAGGGTATGTTGGTCACGAGGAAGGTGGACAACTAACTGAGGCTGTGAGGAGGCGACCTTATTGTGTCATACTCTTTGATGAAGTGGAGAAGGCTCATGTTGCTGTCTTCAACACTCTGCTCCAAGTTTTGGATGATGGTCGATTGACAGACGGGCAAGGCAGGACAGTCGATTTCAGGAACTCGGTGATAATCATGACATCAAACCTTGGTGCTGAACACCTCCTTGCAGGGCTAACTGGGAAAGTAACAATGGAAGTGGCCCGGGACTGTGTGATGCGGGAGGTGAGGAAACACTTCAGACCAGAGCTCTTGAACAGGCTTGACGAGATTGTGGTGTTCGACCCCCTTTCACATGACCAGTTGAGGAAAGTAGCTCGGCTTCAAATGAAAGACGTTGCTGTCCGGCTTGCTGAAAGAGGAGTTGCTTTGGCAGTCACTGATGCTGCTTTGGACTATATCTTGGCAGAGAGTTATGACCCGGTGTATGGTGCTAGGCCTATAAGGAGATGGATGGAGAAGAAGGTGGTGACAGAACTGTCAAAGATGGTTGTGCGTGAGGAAATCGATGAAAACTCCACTGTTTACATAGATGCAGGCGCTGGTGATCTTGTGTACCGGGTAGAAAGTGGAGGTCTAGTGGACGCTTCAACAGGCAAGAAGTCAGATGTGCTGATTCATATTGCTAACGGGCCAAAGAGAAGTGATGCAGCTCAGGCGGTGAAGAAGATGAGGATCGAGGAAATAGAAGATGACGATAATGAGGAAATGATCGAGGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006417 GO:0006950 GO:0006979 GO:0008150 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009636 GO:0009642 GO:0009644 GO:0009889 GO:0009891 GO:0009893 GO:0009941 GO:0009987 GO:0010035 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031967 GO:0031975 GO:0032268 GO:0032270 GO:0033554 GO:0034248 GO:0034250 GO:0034605 GO:0042221 GO:0042493 GO:0042542 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043335 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045727 GO:0046677 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051716 GO:0060255 GO:0065007 GO:0080090 GO:1901700 GO:2000112

KEGG Pathways

Metabolic & Signaling

ko04213 map04213

Pfam Domains

Protein Families

AAA AAA_2 ClpB_D2-small Clp_N

Protein Analysis

911

Amino Acids

101.29

Weight (kDa)

5.81

Isoelectric Point (pI)

40.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Clp_N	PF02861	5 - 129	4.5e-30	Clp repeat (R) N-terminal domain
AAA_16	PF13191	181 - 227	1.4e-06	AAA ATPase domain
NBD_SMAX1	PF23569	185 - 288	6.8e-10	SMAX1 nucleotide binding domain
AAA	PF00004	204 - 317	3e-13	ATPase family associated with various cellular activities (AAA)
AAA_lid_9	PF17871	343 - 442	7.6e-35	AAA lid domain
AAA_2	PF07724	598 - 762	8.3e-68	AAA domain (Cdc48 subfamily)
Sigma54_activat	PF00158	600 - 718	2e-06	Sigma-54 interaction domain
AAA_5	PF07728	602 - 721	1.6e-11	AAA domain (dynein-related subfamily)
AAA	PF00004	603 - 732	4.4e-10	ATPase family associated with various cellular activities (AAA)
ClpB_D2-small	PF10431	768 - 813	3e-08	C-terminal, D2-small domain, of ClpB protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002819)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74310 AT1G74310 AT4G14670
fragaria_vesca	FvH4_5g14020 FvH4_5g14020 FvH4_5g14020 FvH4_5g14020 FvH4_7g22310 FvH4_7g22310 FvH4_7g22310
malus_domestica	MD06G1201600.v1.1 MD14G1210600.v1.1 MD14G1211000.v1.1
prunus_persica	Prupe.5G203700_v2.0.a1
pyrus_communis	pycom06g17750 pycom14g17400
rosa_chinensis	RchiOBHm_Chr1g0366791 RchiOBHm_Chr7g0182871 RchiOBHm_Chr7g0182901
rosa_laevigata	RLG00000005096 RLG00000005098 RLG00000027319
rosa_multiflora	Rmu_sc0000014.1_g000026 Rmu_sc0000318.1_g000026
rosa_roxburghii	Rroxscaffold_3G00230950 Rroxscaffold_3G00270960
rosa_rugosa	Rorug01G0331900 Rorug01G0333200 Rorug06G0451000
rosa_samantha	Rh1AG340000 Rh7AG054200 Rh7AG403300 Rh7BG053700 Rh7CG055300 Rh7DG053500 Rh7DG053600
rosa_wichuraiana	Rw0G008600 Rw1G030200 Rw7G004420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1115
AasI	GACNNNNNNGTC	2 cut(s)	2354, 2491
AccB1I	GGYRCC	1 cut(s)	1948
AccB7I	CCANNNNNTGG	2 cut(s)	632, 2083
AciI	CCGC	6 cut(s)	1019, 1336, 1708, 1730, 2233, 2672
AclI	AACGTT	1 cut(s)	1593
AclWI	GGATC	4 cut(s)	116, 530, 1807, 2696
AcoI	YGGCCR	1 cut(s)	1657
AcsI	RAATTY	2 cut(s)	14, 478
AcuI	CTGAAG	7 cut(s)	450, 831, 891, 1251, 1352, 1587, 2236
AdeI	CACNNNGTG	1 cut(s)	311
AfaI	GTAC	1 cut(s)	2570
AfiI	CCNNNNNNNGG	7 cut(s)	545, 632, 993, 1293, 1945, 2083, 2441
AgeI	ACCGGT	1 cut(s)	128
AjnI	CCWGG	2 cut(s)	625, 1954
Alw21I	GWGCWC	5 cut(s)	289, 1111, 1449, 1845, 2265
Alw26I	GTCTC	4 cut(s)	29, 1353, 1645, 1936
AlwI	GGATC	4 cut(s)	116, 530, 1807, 2696
AlwNI	CAGNNNCTG	3 cut(s)	1745, 2393, 2555
Ama87I	CYCGRG	1 cut(s)	2217
AoxI	GGCC	6 cut(s)	1598, 1657, 1781, 2214, 2450, 2645
ApoI	RAATTY	2 cut(s)	14, 478
AseI	ATTAAT	1 cut(s)	713
AsiGI	ACCGGT	1 cut(s)	128
Asp700I	GAANNNNTTC	2 cut(s)	1561, 2249
AspLEI	GCGC	2 cut(s)	1517, 2555
AspS9I	GGNCC	4 cut(s)	1598, 1817, 2215, 2645
AsuC2I	CCSGG	4 cut(s)	2218, 2219, 2435, 2573
AsuHPI	GGTGA	6 cut(s)	323, 2152, 2251, 2494, 2570, 2686
AsuII	TTCGAA	1 cut(s)	1420
AsuNHI	GCTAGC	1 cut(s)	58
AvaI	CYCGRG	1 cut(s)	2217
AvaII	GGWCC	1 cut(s)	1817
BaeGI	GKGCMC	2 cut(s)	690, 1951
BalI	TGGCCA	1 cut(s)	1659
BanI	GGYRCC	1 cut(s)	1948
BanII	GRGCYC	4 cut(s)	1111, 1449, 1845, 2265
BarI	GAAGNNNNNNTAC	2 cut(s)	2319, 2351
BauI	CACGAG	2 cut(s)	306, 1970
BbsI	GAAGAC	2 cut(s)	341, 2053
Bbv12I	GWGCWC	5 cut(s)	289, 1111, 1449, 1845, 2265
BbvCI	CCTCAGC	1 cut(s)	1614
BccI	CCATC	5 cut(s)	874, 2084, 2457, 2461, 2494
BceAI	ACGGC	2 cut(s)	417, 1611
BcgI	CGANNNNNNTGC	4 cut(s)	23, 57, 2104, 2138
BciT130I	CCWGG	2 cut(s)	627, 1956
BciVI	GTATCC	1 cut(s)	1058
BclI	TGATCA	1 cut(s)	195
BcnI	CCSGG	4 cut(s)	2218, 2219, 2435, 2573
BcoDI	GTCTC	4 cut(s)	29, 1353, 1645, 1936
BfaI	CTAG	6 cut(s)	59, 153, 261, 915, 2448, 2591
BfmI	CTRYAG	1 cut(s)	45
BfoI	RGCGCY	1 cut(s)	2556
BfuI	GTATCC	1 cut(s)	1058
BlpI	GCTNAGC	2 cut(s)	1125, 1860
Bme1390I	CCNGG	6 cut(s)	627, 1956, 2218, 2219, 2435, 2573
Bme18I	GGWCC	1 cut(s)	1817
BmeT110I	CYCGRG	1 cut(s)	2217
BmgT120I	GGNCC	4 cut(s)	1598, 1817, 2215, 2645
BmiI	GGNNCC	3 cut(s)	1001, 1023, 1950
BmrFI	CCNGG	6 cut(s)	627, 1956, 2218, 2219, 2435, 2573
BmrI	ACTGGG	1 cut(s)	2202
BmsI	GCATC	9 cut(s)	874, 920, 970, 1251, 1569, 2220, 2386, 2536, 2650
BmtI	GCTAGC	1 cut(s)	62
BmuI	ACTGGG	1 cut(s)	2202
BpiI	GAAGAC	2 cut(s)	341, 2053
BplI	GAGNNNNNCTC	2 cut(s)	1431, 1463
Bpu10I	CCTNAGC	2 cut(s)	1614, 2667
Bpu1102I	GCTNAGC	2 cut(s)	1125, 1860
Bpu14I	TTCGAA	1 cut(s)	1420
BpuEI	CTTGAG	3 cut(s)	126, 273, 1046
BpuMI	CCSGG	4 cut(s)	2218, 2219, 2435, 2573
Bsa29I	ATCGAT	1 cut(s)	2520
BsaBI	GATNNNNATC	1 cut(s)	2687
BsaJI	CCNNGG	7 cut(s)	757, 1287, 1813, 1848, 1955, 2161, 2217
BsaWI	WCCGGW	2 cut(s)	128, 1162
BsaXI	ACNNNNNCTCC	2 cut(s)	2577, 2607
Bsc4I	CCNNNNNNNGG	7 cut(s)	545, 632, 993, 1293, 1945, 2083, 2441
Bse118I	RCCGGY	2 cut(s)	128, 158
Bse1I	ACTGG	7 cut(s)	334, 1153, 1643, 1801, 1828, 2197, 2314
Bse3DI	GCAATG	1 cut(s)	1608
Bse8I	GATNNNNATC	1 cut(s)	2687
BseBI	CCWGG	2 cut(s)	627, 1956
BseCI	ATCGAT	1 cut(s)	2520
BseDI	CCNNGG	7 cut(s)	757, 1287, 1813, 1848, 1955, 2161, 2217
BseGI	GGATG	4 cut(s)	889, 1266, 2092, 2472
BseJI	GATNNNNATC	1 cut(s)	2687
BseLI	CCNNNNNNNGG	7 cut(s)	545, 632, 993, 1293, 1945, 2083, 2441
BseMI	GCAATG	1 cut(s)	1608
BseNI	ACTGG	7 cut(s)	334, 1153, 1643, 1801, 1828, 2197, 2314
BseRI	GAGGAG	7 cut(s)	222, 572, 604, 774, 1009, 2017, 2388
BseSI	GKGCMC	2 cut(s)	690, 1951
BshFI	GGCC	6 cut(s)	1600, 1659, 1783, 2216, 2452, 2647
BshNI	GGYRCC	1 cut(s)	1948
BshTI	ACCGGT	1 cut(s)	128
BshVI	ATCGAT	1 cut(s)	2520
BsiHKAI	GWGCWC	5 cut(s)	289, 1111, 1449, 1845, 2265
BsiHKCI	CYCGRG	1 cut(s)	2217
BsiSI	CCGG	7 cut(s)	129, 159, 1163, 2218, 2359, 2435, 2572
BslFI	GGGAC	4 cut(s)	485, 1092, 1731, 2234
BslI	CCNNNNNNNGG	7 cut(s)	545, 632, 993, 1293, 1945, 2083, 2441
BsmAI	GTCTC	4 cut(s)	29, 1353, 1645, 1936
BsmFI	GGGAC	4 cut(s)	485, 1092, 1731, 2234
BsmI	GAATGC	1 cut(s)	73
BsnI	GGCC	6 cut(s)	1600, 1659, 1783, 2216, 2452, 2647
BsoBI	CYCGRG	1 cut(s)	2217
Bsp119I	TTCGAA	1 cut(s)	1420
Bsp1286I	GDGCHC	7 cut(s)	289, 690, 1111, 1449, 1845, 1951, 2265
Bsp143I	GATC	9 cut(s)	114, 121, 195, 535, 1501, 1799, 2560, 2688, 2724
Bsp1720I	GCTNAGC	2 cut(s)	1125, 1860
BspACI	CCGC	6 cut(s)	1019, 1336, 1708, 1730, 2233, 2672
BspANI	GGCC	6 cut(s)	1600, 1659, 1783, 2216, 2452, 2647
BspDI	ATCGAT	1 cut(s)	2520
BspHI	TCATGA	2 cut(s)	52, 2148
BspLI	GGNNCC	3 cut(s)	1001, 1023, 1950
BspOI	GCTAGC	1 cut(s)	62
BspPI	GGATC	4 cut(s)	116, 530, 1807, 2696
BspQI	GCTCTTC	1 cut(s)	1437
BspT104I	TTCGAA	1 cut(s)	1420
BspT107I	GGYRCC	1 cut(s)	1948
BsrDI	GCAATG	1 cut(s)	1608
BsrFI	RCCGGY	2 cut(s)	128, 158
BsrI	ACTGG	7 cut(s)	334, 1153, 1643, 1801, 1828, 2197, 2314
BssAI	RCCGGY	2 cut(s)	128, 158
BssECI	CCNNGG	7 cut(s)	757, 1287, 1813, 1848, 1955, 2161, 2217
BssMI	GATC	9 cut(s)	114, 121, 195, 535, 1501, 1799, 2560, 2688, 2724
BssSI	CACGAG	2 cut(s)	306, 1970
BssT1I	CCWWGG	4 cut(s)	1287, 1813, 1848, 2161
Bst2BI	CACGAG	2 cut(s)	306, 1970
Bst2UI	CCWGG	2 cut(s)	627, 1956
Bst4CI	ACNGT	5 cut(s)	695, 2122, 2225, 2493, 2536
Bst6I	CTCTTC	6 cut(s)	499, 562, 1227, 1250, 1437, 1563
BstAPI	GCANNNNNTGC	2 cut(s)	77, 1496
BstBI	TTCGAA	1 cut(s)	1420
BstC8I	GCNNGC	7 cut(s)	60, 522, 862, 1312, 1845, 2364, 2551
BstENI	CCTNNNNNAGG	2 cut(s)	991, 1291
BstF5I	GGATG	4 cut(s)	889, 1266, 2092, 2472
BstH2I	RGCGCY	1 cut(s)	2556
BstHHI	GCGC	2 cut(s)	1517, 2555
BstKTI	GATC	9 cut(s)	117, 124, 198, 538, 1504, 1802, 2563, 2691, 2727
BstMAI	GTCTC	4 cut(s)	29, 1353, 1645, 1936
BstMBI	GATC	9 cut(s)	114, 121, 195, 535, 1501, 1799, 2560, 2688, 2724
BstNI	CCWGG	2 cut(s)	627, 1956
BstNSI	RCATGY	1 cut(s)	80
BstSCI	CCNGG	6 cut(s)	625, 1954, 2216, 2217, 2433, 2571
BstSFI	CTRYAG	1 cut(s)	45
BstSLI	GKGCMC	2 cut(s)	690, 1951
BstV2I	GAAGAC	2 cut(s)	341, 2053
Bsu15I	ATCGAT	1 cut(s)	2520
BsuI	GTATCC	1 cut(s)	1058
BsuRI	GGCC	6 cut(s)	1600, 1659, 1783, 2216, 2452, 2647
BsuTUI	ATCGAT	1 cut(s)	2520
BtsCI	GGATG	4 cut(s)	889, 1266, 2092, 2472
BtsIMutI	CAGTG	3 cut(s)	1650, 2391, 2532
Cac8I	GCNNGC	7 cut(s)	60, 522, 862, 1312, 1845, 2364, 2551
CaiI	CAGNNNCTG	3 cut(s)	1745, 2393, 2555
CciI	TCATGA	2 cut(s)	52, 2148
CfoI	GCGC	2 cut(s)	1517, 2555
Cfr10I	RCCGGY	2 cut(s)	128, 158
Cfr13I	GGNCC	4 cut(s)	1598, 1817, 2215, 2645
Cfr9I	CCCGGG	1 cut(s)	2217
ClaI	ATCGAT	1 cut(s)	2520
CseI	GACGC	2 cut(s)	815, 2606
Csp6I	GTAC	1 cut(s)	2569
CspAI	ACCGGT	1 cut(s)	128
CspCI	CAANNNNNGTGG	2 cut(s)	2193, 2228
CviAII	CATG	9 cut(s)	53, 77, 727, 907, 1085, 1450, 2051, 2149, 2309
CviQI	GTAC	1 cut(s)	2569
DpnI	GATC	9 cut(s)	116, 123, 197, 537, 1503, 1801, 2562, 2690, 2726
DpnII	GATC	9 cut(s)	114, 121, 195, 535, 1501, 1799, 2560, 2688, 2724
DraI	TTTAAA	1 cut(s)	492
DraIII	CACNNNGTG	1 cut(s)	311
DrdI	GACNNNNNNGTC	2 cut(s)	2354, 2491
DseDI	GACNNNNNNGTC	2 cut(s)	2354, 2491
EaeI	YGGCCR	1 cut(s)	1657
Eam1104I	CTCTTC	6 cut(s)	499, 562, 1227, 1250, 1437, 1563
EarI	CTCTTC	6 cut(s)	499, 562, 1227, 1250, 1437, 1563
Ecl136II	GAGCTC	4 cut(s)	1109, 1447, 1843, 2263
Eco130I	CCWWGG	4 cut(s)	1287, 1813, 1848, 2161
Eco147I	AGGCCT	1 cut(s)	2452
Eco24I	GRGCYC	4 cut(s)	1111, 1449, 1845, 2265
Eco47I	GGWCC	1 cut(s)	1817
Eco53kI	GAGCTC	4 cut(s)	1109, 1447, 1843, 2263
Eco57I	CTGAAG	7 cut(s)	450, 831, 891, 1251, 1352, 1587, 2236
Eco88I	CYCGRG	1 cut(s)	2217
EcoICRI	GAGCTC	4 cut(s)	1109, 1447, 1843, 2263
EcoNI	CCTNNNNNAGG	2 cut(s)	991, 1291
EcoRII	CCWGG	2 cut(s)	625, 1954
EcoT14I	CCWWGG	4 cut(s)	1287, 1813, 1848, 2161
EcoT22I	ATGCAT	1 cut(s)	935
EcoT38I	GRGCYC	4 cut(s)	1111, 1449, 1845, 2265
ErhI	CCWWGG	4 cut(s)	1287, 1813, 1848, 2161
FaeI	CATG	9 cut(s)	56, 80, 730, 910, 1088, 1453, 2054, 2152, 2312
FalI	AAGNNNNNCTT	2 cut(s)	1308, 1340
FaqI	GGGAC	4 cut(s)	485, 1092, 1731, 2234
FatI	CATG	9 cut(s)	52, 76, 726, 906, 1084, 1449, 2050, 2148, 2308
FauI	CCCGC	2 cut(s)	1701, 2226
FbaI	TGATCA	1 cut(s)	195
FokI	GGATG	4 cut(s)	896, 1273, 2099, 2479
FriOI	GRGCYC	4 cut(s)	1111, 1449, 1845, 2265
FspBI	CTAG	6 cut(s)	59, 153, 261, 915, 2448, 2591
GlaI	GCGC	2 cut(s)	1516, 2554
HaeII	RGCGCY	1 cut(s)	2556
HaeIII	GGCC	6 cut(s)	1600, 1659, 1783, 2216, 2452, 2647
HapII	CCGG	7 cut(s)	129, 159, 1163, 2218, 2359, 2435, 2572
HgaI	GACGC	2 cut(s)	815, 2606
HhaI	GCGC	2 cut(s)	1517, 2555
Hin1II	CATG	9 cut(s)	56, 80, 730, 910, 1088, 1453, 2054, 2152, 2312
Hin6I	GCGC	2 cut(s)	1515, 2553
HinP1I	GCGC	2 cut(s)	1515, 2553
HincII	GTYRAC	2 cut(s)	331, 1226
HindII	GTYRAC	2 cut(s)	331, 1226
HindIII	AAGCTT	5 cut(s)	215, 428, 486, 530, 1364
HpaII	CCGG	7 cut(s)	129, 159, 1163, 2218, 2359, 2435, 2572
HphI	GGTGA	6 cut(s)	323, 2152, 2251, 2494, 2570, 2686
Hpy166II	GTNNAC	6 cut(s)	331, 1226, 1982, 2539, 2569, 2596
Hpy188I	TCNGA	9 cut(s)	121, 300, 418, 1567, 1747, 1896, 1909, 2255, 2620
Hpy8I	GTNNAC	6 cut(s)	331, 1226, 1982, 2539, 2569, 2596
Hpy99I	CGWCG	2 cut(s)	285, 403
HpyCH4III	ACNGT	5 cut(s)	695, 2122, 2225, 2493, 2536
HpyCH4IV	ACGT	2 cut(s)	1593, 2349
HpySE526I	ACGT	2 cut(s)	1593, 2349
Hsp92II	CATG	9 cut(s)	56, 80, 730, 910, 1088, 1453, 2054, 2152, 2312
HspAI	GCGC	2 cut(s)	1515, 2553
Ksp22I	TGATCA	1 cut(s)	195
Kzo9I	GATC	9 cut(s)	114, 121, 195, 535, 1501, 1799, 2560, 2688, 2724
LguI	GCTCTTC	1 cut(s)	1437
LmnI	GCTCC	2 cut(s)	1021, 2079
LweI	GCATC	9 cut(s)	874, 920, 970, 1251, 1569, 2220, 2386, 2536, 2650
MaeI	CTAG	6 cut(s)	59, 153, 261, 915, 2448, 2591
MaeII	ACGT	2 cut(s)	1593, 2349
MaeIII	GTNAC	7 cut(s)	303, 1139, 1645, 1967, 2200, 2389, 2482
MalI	GATC	9 cut(s)	116, 123, 197, 537, 1503, 1801, 2562, 2690, 2726
MboI	GATC	9 cut(s)	114, 121, 195, 535, 1501, 1799, 2560, 2688, 2724
MfeI	CAATTG	3 cut(s)	39, 1173, 1539
MhlI	GDGCHC	7 cut(s)	289, 690, 1111, 1449, 1845, 1951, 2265
MlsI	TGGCCA	1 cut(s)	1659
MluNI	TGGCCA	1 cut(s)	1659
MlyI	GAGTC	3 cut(s)	579, 1053, 1218
MmeI	TCCRAC	4 cut(s)	702, 1027, 1610, 1887
Mox20I	TGGCCA	1 cut(s)	1659
Mph1103I	ATGCAT	1 cut(s)	935
MroXI	GAANNNNTTC	2 cut(s)	1561, 2249
MscI	TGGCCA	1 cut(s)	1659
MseI	TTAA	5 cut(s)	270, 491, 713, 1428, 2734
MslI	CAYNNNNRTG	1 cut(s)	1086
Msp20I	TGGCCA	1 cut(s)	1659
MspA1I	CMGCKG	4 cut(s)	644, 1129, 1267, 1867
MspI	CCGG	7 cut(s)	129, 159, 1163, 2218, 2359, 2435, 2572
MspR9I	CCNGG	6 cut(s)	627, 1956, 2218, 2219, 2435, 2573
MunI	CAATTG	3 cut(s)	39, 1173, 1539
Mva1269I	GAATGC	1 cut(s)	73
MvaI	CCWGG	2 cut(s)	627, 1956
NciI	CCSGG	4 cut(s)	2218, 2219, 2435, 2573
NdeII	GATC	9 cut(s)	114, 121, 195, 535, 1501, 1799, 2560, 2688, 2724
NheI	GCTAGC	1 cut(s)	58
NlaIII	CATG	9 cut(s)	56, 80, 730, 910, 1088, 1453, 2054, 2152, 2312
NlaIV	GGNNCC	3 cut(s)	1001, 1023, 1950
NmeAIII	GCCGAG	1 cut(s)	2311
NmuCI	GTSAC	5 cut(s)	303, 1645, 1967, 2389, 2482
NsiI	ATGCAT	1 cut(s)	935
NspI	RCATGY	1 cut(s)	80
NspV	TTCGAA	1 cut(s)	1420
PagI	TCATGA	2 cut(s)	52, 2148
PceI	AGGCCT	1 cut(s)	2452
PciSI	GCTCTTC	1 cut(s)	1437
PcsI	WCGNNNNNNNCGW	1 cut(s)	593
PctI	GAATGC	1 cut(s)	73
PdmI	GAANNNNTTC	2 cut(s)	1561, 2249
PflMI	CCANNNNNTGG	2 cut(s)	632, 2083
PinAI	ACCGGT	1 cut(s)	128
PleI	GAGTC	3 cut(s)	578, 1053, 1217
PpsI	GAGTC	3 cut(s)	578, 1053, 1217
PshBI	ATTAAT	1 cut(s)	713
PsiI	TTATAA	1 cut(s)	1115
Psp124BI	GAGCTC	4 cut(s)	1111, 1449, 1845, 2265
Psp1406I	AACGTT	1 cut(s)	1593
Psp6I	CCWGG	2 cut(s)	625, 1954
PspGI	CCWGG	2 cut(s)	625, 1954
PspN4I	GGNNCC	3 cut(s)	1001, 1023, 1950
PspPI	GGNCC	4 cut(s)	1598, 1817, 2215, 2645
PstNI	CAGNNNCTG	3 cut(s)	1745, 2393, 2555
PvuII	CAGCTG	4 cut(s)	644, 1129, 1267, 1867
RsaI	GTAC	1 cut(s)	2570
RsaNI	GTAC	1 cut(s)	2569
RseI	CAYNNNNRTG	1 cut(s)	1086
SacI	GAGCTC	4 cut(s)	1111, 1449, 1845, 2265
SapI	GCTCTTC	1 cut(s)	1437
SaqAI	TTAA	5 cut(s)	270, 491, 713, 1428, 2734
Sau3AI	GATC	9 cut(s)	114, 121, 195, 535, 1501, 1799, 2560, 2688, 2724
Sau96I	GGNCC	4 cut(s)	1598, 1817, 2215, 2645
SchI	GAGTC	3 cut(s)	579, 1053, 1218
ScrFI	CCNGG	6 cut(s)	627, 1956, 2218, 2219, 2435, 2573
SduI	GDGCHC	7 cut(s)	289, 690, 1111, 1449, 1845, 1951, 2265
SfaNI	GCATC	9 cut(s)	874, 920, 970, 1251, 1569, 2220, 2386, 2536, 2650
SfcI	CTRYAG	1 cut(s)	45
SfuI	TTCGAA	1 cut(s)	1420
SinI	GGWCC	1 cut(s)	1817
SmaI	CCCGGG	1 cut(s)	2219
SmiMI	CAYNNNNRTG	1 cut(s)	1086
SmlI	CTYRAG	3 cut(s)	141, 288, 1061
SmoI	CTYRAG	3 cut(s)	141, 288, 1061
SseBI	AGGCCT	1 cut(s)	2452
SsiI	CCGC	6 cut(s)	1019, 1336, 1708, 1730, 2233, 2672
SspMI	CTAG	6 cut(s)	59, 153, 261, 915, 2448, 2591
SstI	GAGCTC	4 cut(s)	1111, 1449, 1845, 2265
StuI	AGGCCT	1 cut(s)	2452
StyD4I	CCNGG	6 cut(s)	625, 1954, 2216, 2217, 2433, 2571
StyI	CCWWGG	4 cut(s)	1287, 1813, 1848, 2161
TaaI	ACNGT	5 cut(s)	695, 2122, 2225, 2493, 2536
TaiI	ACGT	2 cut(s)	1596, 2352
Tru1I	TTAA	5 cut(s)	270, 491, 713, 1428, 2734
Tru9I	TTAA	5 cut(s)	270, 491, 713, 1428, 2734
TscAI	CASTG	3 cut(s)	1650, 2398, 2539
TseFI	GTSAC	5 cut(s)	303, 1645, 1967, 2389, 2482
Tsp45I	GTSAC	5 cut(s)	303, 1645, 1967, 2389, 2482
TspDTI	ATGAA	8 cut(s)	17, 833, 1397, 1893, 2049, 2357, 2538, 2621
TspMI	CCCGGG	1 cut(s)	2217
TspRI	CASTG	3 cut(s)	1650, 2398, 2539
Van91I	CCANNNNNTGG	2 cut(s)	632, 2083
VpaK11BI	GGWCC	1 cut(s)	1817
VspI	ATTAAT	1 cut(s)	713
XagI	CCTNNNNNAGG	2 cut(s)	991, 1291
XapI	RAATTY	2 cut(s)	14, 478
XceI	RCATGY	1 cut(s)	80
XmaI	CCCGGG	1 cut(s)	2217
XmnI	GAANNNNTTC	2 cut(s)	1561, 2249
XspI	CTAG	6 cut(s)	59, 153, 261, 915, 2448, 2591
Zsp2I	ATGCAT	1 cut(s)	935

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.