Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0002819

Physical Location & Seq

Genomic Coordinates Reverse (-)

29651352 .. 29656826

Gene Structure 5475 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1211000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2637 bp

ATGAACCCTGATAAGTTTACCCACAAGACCAATGAGACTATTGCCGGAGCTCATGTCCTGGCCATGGATGCTGGCCACGCGCAGTTCACGCCACTCCATCTTGCAACAGCATTGGTATCTGATCCCAGTGGCATTTTCAACCAAGCCATTGCCAATGCCGGTGGCAGTGTTGACACTCCGAAGTCTGTGGAGAGGGTGTTCAACCAGGCCCTGAAGAAGCTCCCATCTCAGTCACCTCCGCCGTATGAAATCCTAGCCAGTTCATCTCTCATCAAAGTGATTAGGAGAGCTCAGTCAGCGCAGAAGACAAGAGGGGACACACACTTGGCCGTTGATCAGTTGATCTTTAGTCTTCTTGAGGATTCCCAGATTGGTGACTTGTTGAAGGAAGCCGGCATTGCCACGGCCAGAATGAAATCTGAAGTCGAAAAGCTTCGTGGGAAGGAAGGGAAGAAGGTGGAGAGTGCTTCTGGGGATACTACATTTCAGGCATTGAAGACTTATGGACGGGACCTTGTTGAGCAGGCCGGGAAACTTGACCCGGTTATTGGCCGTGACGAGGAGATAAGGAGAGTGGTGAGGATTCTTTCGAGGAGGACTAAGAACAACCCTGTTCTAATTGGAGAGCCGGGAGTTGGGAAGACTGCTGTGGTTGAAGGGCTGGCTCAGAGGATAGTTGGCGGGGATGTCCCAAGTAATTTGGCTGATGTAAGGCTCATTGCATTGGATATGGGGGCCAAGTATCGAGGGAAGTTTGAGGAGCGGTTAAAGGCGGTTGTGAAGGAAGTGGAAGAGGCTGAGGGGAAGGTGATTCTGTTTATTGATGAAATTCACCTTGTTCTTGGCGCCGGAAGGACGGAAGGATCCATGGATGCAGGCAACCTGTTCAAGCCAACGCTCGCACGGGGGCAACTTCGGTACATTGGCGCAACAACTCTTGAGGAGTACAGAAAATATGTAGACAAAGATGCTGCATTTGAGAGAAGGTTCCAGCAGGTTTATGTGGCGGAGCCTAGTGTTCCGGATACAATTAGCATCCTTCGAGGTTTGAAAGAGAGGTATGAGGATCACCACTGTGTTCGGATTCTGGACAGAACTCTTGTTGTTGCTGCACAGCTTTCGAGTCGATACATCACAGGGCGACATCTCCCTGACAAGGCGATTGATCTAGTTGACGAAGCTTGTGCGAATGTGAGAGTCCAACTTGACAGCCAACCTGAAGAAATCGATAACCTCGAAAGGAAGAGGATGCAGCTGGAAGTTGAGCTTCATGCACTTGAGAAAGAAAAGGACAAAGCTAGCAAAGCTCGTCTTGTCGAGGTGCGAAAAGAGCTTGATGAACTGAGAGACAAGCTTCAGCCACTGGTGATGAAGTATCGAAAGGAGAAGGAAAGGGTGGACGAGCTTCGAAAGCTTAAGCAGAAGCGTGAAGAGCTCTTGGTAGCTTTAGCAGAGGCCGAAAGAAGATATGATTTGGCTAGGGCTGCTGACTTGAGTTGTGGCCCTATTCAGGAAGTGGAAACCACCATTGCAAAGCTTGAAGGTAGTACTGCCGAAAACTTGATTTTGACAGAGACGGTTGGACCAGAACAAATTGCAGAGGTGGTTAGCCGTTGGACCGGCATACCTGTCACACGGCTTGGCCAGAATGACAAGGAAAGGTTAATTGGACTTGCGGAAAGGTTGCATAAGAGAGTAGTGGGGCAAGACCAGGCAGTTGATGCTGTCGCCGAGGCGGTTTTAATGTCAAGAGCGGGGCTAGGAAGGCCACAACAACCTACTGGTTCATTCCTCTTCTTGGGCCCAACTGGTGTCGGTAAAACCGAGCTTGCGAAGGCGCTTGCTGAACAACTTTTTGATGACGAGAATCTGATTGTCAGAATTGACATGTCCGAGTATATGGAACAACATTCTGTCTCGCGCTTAATTGACACTCCCCCTGGGTATATTGGTCGCAATGAAGGCGGGCAACTCACGGAGGCCGTGAGGCGGAGGCCTTACAGTGTTGTATTGTTTGATGAAGTGGAGAAGGCTCACACCGCTGCTTTCAACACACTCCTGCAAGTCTTGGATGATGGAAGACTAACCGATGGACAAGGCCGTACAGTTGATTTCAGAAACACGGTGATTGTCATGACTTCAAACTTGGGAGCAGAACATCTCCTCTCCGGTTCGATGGGCAAGTGCTCGATGCAGGTTGCTCACGATGAAGTTATGAAAGAGGTGAGAAGGCACTTTAGCCCTGAGCTGCTGAACCATCTCGATGAGATTGTTGTATTTGACCCTCTCTCTCGCGATCAACTGAGGAAGGTTGCCAGATTGCAAATGAAAGATGTTGCAGCCCGACTGGCCGAGAAGGGCATTGCTATGGCAGTCACCGATGCAGCGTTGGATTACATTCTGGGCGAGAGTTATGATCCCGTTTACGGTGCCAGACCTATTAGGAGATGGCTGGAGAAGAAGGTGGTGAAGGAGTTGTCGCGGATGCTTGTGCGAGAAGAAATAGATGAAAACTCGACCGTGTTCATAGATGCAGCGCCAAGCGGAAGCGGGCTTGTGTACAGGGTTGAGAAGAACGGAGGGCTTGTGGATGCGGCGATGGGGCAGAAGTCGGATGCGATGCCGATCAGGCTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006417 GO:0006950 GO:0006979 GO:0008150 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009636 GO:0009642 GO:0009644 GO:0009889 GO:0009891 GO:0009893 GO:0009941 GO:0009987 GO:0010035 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031967 GO:0031975 GO:0032268 GO:0032270 GO:0033554 GO:0034248 GO:0034250 GO:0034605 GO:0042221 GO:0042493 GO:0042542 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043335 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045727 GO:0046677 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051716 GO:0060255 GO:0065007 GO:0080090 GO:1901700 GO:2000112

KEGG Pathways

Metabolic & Signaling

ko04213 map04213

Pfam Domains

Protein Families

AAA AAA_2 ClpB_D2-small Clp_N

Protein Analysis

879

Amino Acids

97.44

Weight (kDa)

6.2

Isoelectric Point (pI)

34.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Clp_N	PF02861	5 - 130	9e-27	Clp repeat (R) N-terminal domain
AAA_16	PF13191	182 - 227	2.9e-06	AAA ATPase domain
NBD_SMAX1	PF23569	186 - 281	6.5e-07	SMAX1 nucleotide binding domain
AAA	PF00004	205 - 301	3.4e-11	ATPase family associated with various cellular activities (AAA)
AAA_lid_9	PF17871	339 - 440	2.8e-33	AAA lid domain
AAA_2	PF07724	593 - 757	2.5e-63	AAA domain (Cdc48 subfamily)
AAA_5	PF07728	597 - 716	1.9e-12	AAA domain (dynein-related subfamily)
AAA	PF00004	598 - 721	2.3e-09	ATPase family associated with various cellular activities (AAA)
ClpB_D2-small	PF10431	763 - 842	3e-22	C-terminal, D2-small domain, of ClpB protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002819)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74310 AT1G74310 AT4G14670
fragaria_vesca	FvH4_5g14020 FvH4_5g14020 FvH4_5g14020 FvH4_5g14020 FvH4_7g22310 FvH4_7g22310 FvH4_7g22310
malus_domestica	MD06G1201600.v1.1 MD14G1210600.v1.1 MD14G1211000.v1.1
prunus_persica	Prupe.5G203700_v2.0.a1
pyrus_communis	pycom06g17750 pycom14g17400
rosa_chinensis	RchiOBHm_Chr1g0366791 RchiOBHm_Chr7g0182871 RchiOBHm_Chr7g0182901
rosa_laevigata	RLG00000005096 RLG00000005098 RLG00000027319
rosa_multiflora	Rmu_sc0000014.1_g000026 Rmu_sc0000318.1_g000026
rosa_roxburghii	Rroxscaffold_3G00230950 Rroxscaffold_3G00270960
rosa_rugosa	Rorug01G0331900 Rorug01G0333200 Rorug06G0451000
rosa_samantha	Rh1AG340000 Rh7AG054200 Rh7AG403300 Rh7BG053700 Rh7CG055300 Rh7DG053500 Rh7DG053600
rosa_wichuraiana	Rw0G008600 Rw1G030200 Rw7G004420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	985, 2185
AccB1I	GGYRCC	2 cut(s)	845, 2429
AccBSI	CCGCTC	2 cut(s)	763, 1754
AccI	GTMKAC	1 cut(s)	960
AccII	CGCG	4 cut(s)	80, 1921, 2295, 2482
AccIII	TCCGGA	1 cut(s)	1021
AclWI	GGATC	5 cut(s)	116, 858, 871, 1074, 2411
AcoI	YGGCCR	7 cut(s)	60, 73, 327, 405, 550, 1642, 2349
AcsI	RAATTY	1 cut(s)	828
AcuI	CTGAAG	4 cut(s)	233, 441, 1239, 1340
AcyI	GRCGYC	1 cut(s)	846
AfaI	GTAC	5 cut(s)	920, 947, 1549, 2104, 2561
AfiI	CCNNNNNNNGG	9 cut(s)	64, 548, 559, 635, 1156, 1510, 1798, 1989, 2426
AflII	CTTAAG	1 cut(s)	1415
AflIII	ACRYGT	1 cut(s)	1887
AjnI	CCWGG	4 cut(s)	57, 204, 1710, 1939
AjuI	GAANNNNNNNTTGG	2 cut(s)	1650, 1682
AleI	CACNNNNGTG	1 cut(s)	1074
AloI	GAACNNNNNNTCC	2 cut(s)	182, 214
Alw21I	GWGCWC	4 cut(s)	52, 292, 1437, 2189
Alw26I	GTCTC	4 cut(s)	29, 1341, 1568, 1921
AlwI	GGATC	5 cut(s)	116, 858, 871, 1074, 2411
AlwNI	CAGNNNCTG	2 cut(s)	211, 1363
Aor13HI	TCCGGA	1 cut(s)	1021
ApaI	GGGCCC	1 cut(s)	1805
ApeKI	GCWGC	9 cut(s)	971, 1109, 1252, 1484, 2042, 2248, 2339, 2384, 2534
ApoI	RAATTY	1 cut(s)	828
Asp700I	GAANNNNTTC	1 cut(s)	432
AspLEI	GCGC	7 cut(s)	82, 301, 848, 929, 1840, 1923, 2539
AspS9I	GGNCC	8 cut(s)	208, 511, 735, 1502, 1583, 1617, 1801, 1802
AsuC2I	CCSGG	3 cut(s)	529, 542, 630
AsuII	TTCGAA	1 cut(s)	1408
AsuNHI	GCTAGC	1 cut(s)	1298
AvaII	GGWCC	3 cut(s)	511, 1583, 1617
BaeGI	GKGCMC	1 cut(s)	1805
BalI	TGGCCA	3 cut(s)	62, 75, 1644
BamHI	GGATCC	1 cut(s)	863
BanI	GGYRCC	2 cut(s)	845, 2429
BanII	GRGCYC	4 cut(s)	52, 292, 1437, 1805
BbsI	GAAGAC	5 cut(s)	311, 344, 503, 647, 2086
Bbv12I	GWGCWC	4 cut(s)	52, 292, 1437, 2189
BbvCI	CCTCAGC	1 cut(s)	798
BbvI	GCAGC	9 cut(s)	958, 1096, 1264, 1471, 2029, 2235, 2351, 2396, 2546
BccI	CCATC	8 cut(s)	105, 232, 2069, 2084, 2170, 2265, 2442, 2593
BceAI	ACGGC	8 cut(s)	226, 314, 420, 537, 1596, 1652, 1967, 2085
BcgI	CGANNNNNNTGC	4 cut(s)	1101, 1135, 1166, 1200
BciT130I	CCWGG	4 cut(s)	59, 206, 1712, 1941
BciVI	GTATCC	2 cut(s)	469, 1018
BclI	TGATCA	1 cut(s)	334
BcnI	CCSGG	3 cut(s)	529, 542, 630
BcoDI	GTCTC	4 cut(s)	29, 1341, 1568, 1921
BfaI	CTAG	6 cut(s)	254, 1014, 1169, 1299, 1479, 1760
BfoI	RGCGCY	3 cut(s)	849, 1841, 2540
BfrI	CTTAAG	1 cut(s)	1415
BfuAI	ACCTGC	2 cut(s)	985, 2185
BfuI	GTATCC	2 cut(s)	469, 1018
BglI	GCCNNNNNGGC	2 cut(s)	2348, 2629
BmcAI	AGTACT	1 cut(s)	1549
Bme1390I	CCNGG	7 cut(s)	59, 206, 529, 542, 630, 1712, 1941
Bme18I	GGWCC	3 cut(s)	511, 1583, 1617
BmgT120I	GGNCC	8 cut(s)	208, 511, 735, 1502, 1583, 1617, 1801, 1802
BmiI	GGNNCC	8 cut(s)	512, 736, 847, 865, 989, 1011, 1803, 2431
BmrFI	CCNGG	7 cut(s)	59, 206, 529, 542, 630, 1712, 1941
BmrI	ACTGGG	1 cut(s)	120
BmtI	GCTAGC	1 cut(s)	1302
BmuI	ACTGGG	1 cut(s)	120
BpiI	GAAGAC	5 cut(s)	311, 344, 503, 647, 2086
BpmI	CTGGAG	1 cut(s)	2474
Bpu10I	CCTNAGC	2 cut(s)	798, 2244
Bpu14I	TTCGAA	1 cut(s)	1408
BpuEI	CTTGAG	4 cut(s)	377, 959, 1298, 1513
BpuMI	CCSGG	3 cut(s)	529, 542, 630
Bsa29I	ATCGAT	1 cut(s)	1227
BsaBI	GATNNNNATC	1 cut(s)	2624
BsaHI	GRCGYC	1 cut(s)	846
BsaJI	CCNNGG	6 cut(s)	63, 402, 867, 1731, 1939, 1940
BsaWI	WCCGGW	2 cut(s)	1021, 2168
BsaXI	ACNNNNNCTCC	6 cut(s)	39, 69, 182, 212, 2571, 2601
Bsc4I	CCNNNNNNNGG	9 cut(s)	64, 548, 559, 635, 1156, 1510, 1798, 1989, 2426
Bse118I	RCCGGY	3 cut(s)	158, 392, 1619
Bse1I	ACTGG	6 cut(s)	126, 258, 1368, 1786, 1813, 2352
Bse3DI	GCAATG	6 cut(s)	147, 396, 717, 1527, 1963, 2361
Bse8I	GATNNNNATC	1 cut(s)	2624
BseAI	TCCGGA	1 cut(s)	1021
BseBI	CCWGG	4 cut(s)	59, 206, 1712, 1941
BseCI	ATCGAT	1 cut(s)	1227
BseDI	CCNNGG	6 cut(s)	63, 402, 867, 1731, 1939, 1940
BseGI	GGATG	9 cut(s)	73, 691, 877, 1035, 1254, 2077, 2490, 2596, 2620
BseJI	GATNNNNATC	1 cut(s)	2624
BseLI	CCNNNNNNNGG	9 cut(s)	64, 548, 559, 635, 1156, 1510, 1798, 1989, 2426
BseMI	GCAATG	6 cut(s)	147, 396, 717, 1527, 1963, 2361
BseMII	CTCAG	7 cut(s)	242, 305, 680, 789, 1334, 2235, 2294
BseNI	ACTGG	6 cut(s)	126, 258, 1368, 1786, 1813, 2352
BseRI	GAGGAG	5 cut(s)	575, 607, 773, 956, 2153
BseSI	GKGCMC	1 cut(s)	1805
BseXI	GCAGC	9 cut(s)	958, 1096, 1264, 1471, 2029, 2235, 2351, 2396, 2546
BsgI	GTGCAG	1 cut(s)	1095
Bsh1236I	CGCG	4 cut(s)	80, 1921, 2295, 2482
Bsh1285I	CGRYCG	1 cut(s)	2520
BshNI	GGYRCC	2 cut(s)	845, 2429
BshVI	ATCGAT	1 cut(s)	1227
BsiEI	CGRYCG	1 cut(s)	2520
BsiHKAI	GWGCWC	4 cut(s)	52, 292, 1437, 2189
BslFI	GGGAC	3 cut(s)	329, 524, 674
BslI	CCNNNNNNNGG	9 cut(s)	64, 548, 559, 635, 1156, 1510, 1798, 1989, 2426
BsmAI	GTCTC	4 cut(s)	29, 1341, 1568, 1921
BsmBI	CGTCTC	1 cut(s)	1568
BsmFI	GGGAC	3 cut(s)	329, 524, 674
Bsp119I	TTCGAA	1 cut(s)	1408
Bsp120I	GGGCCC	1 cut(s)	1801
Bsp1286I	GDGCHC	5 cut(s)	52, 292, 1437, 1805, 2189
Bsp13I	TCCGGA	1 cut(s)	1021
Bsp1407I	TGTACA	1 cut(s)	2559
Bsp143I	GATC	9 cut(s)	121, 334, 342, 863, 1066, 1165, 2296, 2416, 2625
Bsp19I	CCATGG	2 cut(s)	63, 867
Bsp68I	TCGCGA	1 cut(s)	2295
BspCNI	CTCAG	7 cut(s)	241, 304, 679, 790, 1335, 2236, 2295
BspDI	ATCGAT	1 cut(s)	1227
BspEI	TCCGGA	1 cut(s)	1021
BspFNI	CGCG	4 cut(s)	80, 1921, 2295, 2482
BspHI	TCATGA	1 cut(s)	2133
BspLI	GGNNCC	8 cut(s)	512, 736, 847, 865, 989, 1011, 1803, 2431
BspMI	ACCTGC	2 cut(s)	985, 2185
BspOI	GCTAGC	1 cut(s)	1302
BspPI	GGATC	5 cut(s)	116, 858, 871, 1074, 2411
BspQI	GCTCTTC	1 cut(s)	1425
BspT104I	TTCGAA	1 cut(s)	1408
BspT107I	GGYRCC	2 cut(s)	845, 2429
BspTI	CTTAAG	1 cut(s)	1415
BsrBI	CCGCTC	2 cut(s)	763, 1754
BsrDI	GCAATG	6 cut(s)	147, 396, 717, 1527, 1963, 2361
BsrFI	RCCGGY	3 cut(s)	158, 392, 1619
BsrGI	TGTACA	1 cut(s)	2559
BsrI	ACTGG	6 cut(s)	126, 258, 1368, 1786, 1813, 2352
BssAI	RCCGGY	3 cut(s)	158, 392, 1619
BssECI	CCNNGG	6 cut(s)	63, 402, 867, 1731, 1939, 1940
BssMI	GATC	9 cut(s)	121, 334, 342, 863, 1066, 1165, 2296, 2416, 2625
BssNI	GRCGYC	1 cut(s)	846
BssT1I	CCWWGG	2 cut(s)	63, 867
Bst2UI	CCWGG	4 cut(s)	59, 206, 1712, 1941
Bst4CI	ACNGT	7 cut(s)	1076, 1579, 2003, 2107, 2125, 2429, 2521
Bst6I	CTCTTC	4 cut(s)	786, 1238, 1425, 1799
BstACI	GRCGYC	1 cut(s)	846
BstAFI	CTTAAG	1 cut(s)	1415
BstAPI	GCANNNNNTGC	1 cut(s)	1721
BstAUI	TGTACA	1 cut(s)	2559
BstBI	TTCGAA	1 cut(s)	1408
BstDEI	CTNAG	8 cut(s)	228, 291, 600, 666, 798, 1343, 2244, 2303
BstDSI	CCRYGG	3 cut(s)	63, 402, 867
BstF5I	GGATG	9 cut(s)	73, 691, 877, 1035, 1254, 2077, 2490, 2596, 2620
BstFNI	CGCG	4 cut(s)	80, 1921, 2295, 2482
BstH2I	RGCGCY	3 cut(s)	849, 1841, 2540
BstHHI	GCGC	7 cut(s)	82, 301, 848, 929, 1840, 1923, 2539
BstKTI	GATC	9 cut(s)	124, 337, 345, 866, 1069, 1168, 2299, 2419, 2628
BstMAI	GTCTC	4 cut(s)	29, 1341, 1568, 1921
BstMBI	GATC	9 cut(s)	121, 334, 342, 863, 1066, 1165, 2296, 2416, 2625
BstMCI	CGRYCG	1 cut(s)	2520
BstNI	CCWGG	4 cut(s)	59, 206, 1712, 1941
BstNSI	RCATGY	1 cut(s)	1891
BstSCI	CCNGG	7 cut(s)	57, 204, 527, 540, 628, 1710, 1939
BstSLI	GKGCMC	1 cut(s)	1805
BstUI	CGCG	4 cut(s)	80, 1921, 2295, 2482
BstV1I	GCAGC	9 cut(s)	958, 1096, 1264, 1471, 2029, 2235, 2351, 2396, 2546
BstV2I	GAAGAC	5 cut(s)	311, 344, 503, 647, 2086
BstX2I	RGATCY	1 cut(s)	863
BstYI	RGATCY	1 cut(s)	863
Bsu15I	ATCGAT	1 cut(s)	1227
BsuI	GTATCC	2 cut(s)	469, 1018
BsuTUI	ATCGAT	1 cut(s)	1227
BtgI	CCRYGG	3 cut(s)	63, 402, 867
BtgZI	GCGATG	2 cut(s)	2612, 2633
BtsCI	GGATG	9 cut(s)	73, 691, 877, 1035, 1254, 2077, 2490, 2596, 2620
BtsI	GCAGTG	1 cut(s)	172
BtsIMutI	CAGTG	5 cut(s)	133, 172, 1072, 1361, 2008
BtuMI	TCGCGA	1 cut(s)	2295
BveI	ACCTGC	2 cut(s)	985, 2185
CaiI	CAGNNNCTG	2 cut(s)	211, 1363
CciI	TCATGA	1 cut(s)	2133
CfoI	GCGC	7 cut(s)	82, 301, 848, 929, 1840, 1923, 2539
Cfr10I	RCCGGY	3 cut(s)	158, 392, 1619
Cfr13I	GGNCC	8 cut(s)	208, 511, 735, 1502, 1583, 1617, 1801, 1802
ClaI	ATCGAT	1 cut(s)	1227
Csp6I	GTAC	5 cut(s)	919, 946, 1548, 2103, 2560
CspCI	CAANNNNNGTGG	2 cut(s)	142, 177
CviAII	CATG	6 cut(s)	53, 64, 868, 1271, 1888, 2134
CviQI	GTAC	5 cut(s)	919, 946, 1548, 2103, 2560
DdeI	CTNAG	8 cut(s)	228, 291, 600, 666, 798, 1343, 2244, 2303
DinI	GGCGCC	1 cut(s)	847
DpnI	GATC	9 cut(s)	123, 336, 344, 865, 1068, 1167, 2298, 2418, 2627
DpnII	GATC	9 cut(s)	121, 334, 342, 863, 1066, 1165, 2296, 2416, 2625
EaeI	YGGCCR	7 cut(s)	60, 73, 327, 405, 550, 1642, 2349
Eam1104I	CTCTTC	4 cut(s)	786, 1238, 1425, 1799
EarI	CTCTTC	4 cut(s)	786, 1238, 1425, 1799
EciI	GGCGGA	3 cut(s)	228, 1022, 2005
Ecl136II	GAGCTC	3 cut(s)	50, 290, 1435
Eco130I	CCWWGG	2 cut(s)	63, 867
Eco147I	AGGCCT	1 cut(s)	1996
Eco24I	GRGCYC	4 cut(s)	52, 292, 1437, 1805
Eco47I	GGWCC	3 cut(s)	511, 1583, 1617
Eco53kI	GAGCTC	3 cut(s)	50, 290, 1435
Eco57I	CTGAAG	4 cut(s)	233, 441, 1239, 1340
EcoICRI	GAGCTC	3 cut(s)	50, 290, 1435
EcoO109I	RGGNCCY	2 cut(s)	208, 511
EcoRII	CCWGG	4 cut(s)	57, 204, 1710, 1939
EcoT14I	CCWWGG	2 cut(s)	63, 867
EcoT38I	GRGCYC	4 cut(s)	52, 292, 1437, 1805
EgeI	GGCGCC	1 cut(s)	847
EheI	GGCGCC	1 cut(s)	847
ErhI	CCWWGG	2 cut(s)	63, 867
Esp3I	CGTCTC	1 cut(s)	1568
FaeI	CATG	6 cut(s)	56, 67, 871, 1274, 1891, 2137
FalI	AAGNNNNNCTT	4 cut(s)	1251, 1283, 1296, 1328
FaqI	GGGAC	3 cut(s)	329, 524, 674
FatI	CATG	6 cut(s)	52, 63, 867, 1270, 1887, 2133
FauI	CCCGC	4 cut(s)	674, 1747, 1958, 2543
FbaI	TGATCA	1 cut(s)	334
FblI	GTMKAC	1 cut(s)	960
FokI	GGATG	9 cut(s)	80, 698, 884, 1022, 1261, 2084, 2497, 2603, 2627
FriOI	GRGCYC	4 cut(s)	52, 292, 1437, 1805
FspBI	CTAG	6 cut(s)	254, 1014, 1169, 1299, 1479, 1760
GlaI	GCGC	7 cut(s)	81, 300, 847, 928, 1839, 1922, 2538
GsuI	CTGGAG	1 cut(s)	2474
HaeII	RGCGCY	3 cut(s)	849, 1841, 2540
HhaI	GCGC	7 cut(s)	82, 301, 848, 929, 1840, 1923, 2539
Hin1I	GRCGYC	1 cut(s)	846
Hin1II	CATG	6 cut(s)	56, 67, 871, 1274, 1891, 2137
Hin6I	GCGC	7 cut(s)	80, 299, 846, 927, 1838, 1921, 2537
HinP1I	GCGC	7 cut(s)	80, 299, 846, 927, 1838, 1921, 2537
HincII	GTYRAC	2 cut(s)	172, 1174
HindII	GTYRAC	2 cut(s)	172, 1174
HindIII	AAGCTT	5 cut(s)	431, 1179, 1352, 1412, 1535
HinfI	GANTC	7 cut(s)	362, 583, 811, 1084, 1123, 1197, 1867
Hpy166II	GTNNAC	8 cut(s)	18, 87, 172, 961, 1174, 1399, 2425, 2560
Hpy8I	GTNNAC	8 cut(s)	18, 87, 172, 961, 1174, 1399, 2425, 2560
HpyCH4III	ACNGT	7 cut(s)	1076, 1579, 2003, 2107, 2125, 2429, 2521
HpyF3I	CTNAG	8 cut(s)	228, 291, 600, 666, 798, 1343, 2244, 2303
Hsp92I	GRCGYC	1 cut(s)	846
Hsp92II	CATG	6 cut(s)	56, 67, 871, 1274, 1891, 2137
HspAI	GCGC	7 cut(s)	80, 299, 846, 927, 1838, 1921, 2537
KasI	GGCGCC	1 cut(s)	845
Kpn2I	TCCGGA	1 cut(s)	1021
KroI	GCCGGC	1 cut(s)	392
KroNI	GCCGGC	1 cut(s)	394
Ksp22I	TGATCA	1 cut(s)	334
Kzo9I	GATC	9 cut(s)	121, 334, 342, 863, 1066, 1165, 2296, 2416, 2625
LguI	GCTCTTC	1 cut(s)	1425
LmnI	GCTCC	5 cut(s)	47, 225, 760, 1009, 2150
Lsp1109I	GCAGC	9 cut(s)	958, 1096, 1264, 1471, 2029, 2235, 2351, 2396, 2546
MaeI	CTAG	6 cut(s)	254, 1014, 1169, 1299, 1479, 1760
MaeIII	GTNAC	5 cut(s)	231, 374, 554, 1630, 2374
MalI	GATC	9 cut(s)	123, 336, 344, 865, 1068, 1167, 2298, 2418, 2627
MbiI	CCGCTC	2 cut(s)	763, 1754
MboI	GATC	9 cut(s)	121, 334, 342, 863, 1066, 1165, 2296, 2416, 2625
MflI	RGATCY	1 cut(s)	863
MhlI	GDGCHC	5 cut(s)	52, 292, 1437, 1805, 2189
MlsI	TGGCCA	3 cut(s)	62, 75, 1644
MluCI	AATT	8 cut(s)	618, 697, 828, 1029, 1593, 1665, 1881, 1926
MluNI	TGGCCA	3 cut(s)	62, 75, 1644
Mly113I	GGCGCC	1 cut(s)	846
MlyI	GAGTC	2 cut(s)	1132, 1206
MmeI	TCCRAC	5 cut(s)	1225, 1561, 1595, 2370, 2592
Mox20I	TGGCCA	3 cut(s)	62, 75, 1644
MroI	TCCGGA	1 cut(s)	1021
MroNI	GCCGGC	1 cut(s)	392
MroXI	GAANNNNTTC	1 cut(s)	432
MscI	TGGCCA	3 cut(s)	62, 75, 1644
MseI	TTAA	5 cut(s)	767, 1416, 1664, 1742, 1925
MslI	CAYNNNNRTG	4 cut(s)	275, 1074, 2262, 2366
Msp20I	TGGCCA	3 cut(s)	62, 75, 1644
MspA1I	CMGCKG	2 cut(s)	1255, 2042
MspCI	CTTAAG	1 cut(s)	1415
MspR9I	CCNGG	7 cut(s)	59, 206, 529, 542, 630, 1712, 1941
MvaI	CCWGG	4 cut(s)	59, 206, 1712, 1941
MvnI	CGCG	4 cut(s)	80, 1921, 2295, 2482
NaeI	GCCGGC	1 cut(s)	394
NarI	GGCGCC	1 cut(s)	846
NciI	CCSGG	3 cut(s)	529, 542, 630
NcoI	CCATGG	2 cut(s)	63, 867
NdeII	GATC	9 cut(s)	121, 334, 342, 863, 1066, 1165, 2296, 2416, 2625
NgoMIV	GCCGGC	1 cut(s)	392
NheI	GCTAGC	1 cut(s)	1298
NlaIII	CATG	6 cut(s)	56, 67, 871, 1274, 1891, 2137
NlaIV	GGNNCC	8 cut(s)	512, 736, 847, 865, 989, 1011, 1803, 2431
NmeAIII	GCCGAG	2 cut(s)	1756, 2377
NmuCI	GTSAC	5 cut(s)	231, 374, 554, 1630, 2374
NruI	TCGCGA	1 cut(s)	2295
NspI	RCATGY	1 cut(s)	1891
NspV	TTCGAA	1 cut(s)	1408
OliI	CACNNNNGTG	1 cut(s)	1074
PagI	TCATGA	1 cut(s)	2133
PasI	CCCWGGG	1 cut(s)	1940
PceI	AGGCCT	1 cut(s)	1996
PciI	ACATGT	1 cut(s)	1887
PciSI	GCTCTTC	1 cut(s)	1425
PcsI	WCGNNNNNNNCGW	2 cut(s)	1233, 1821
PdiI	GCCGGC	1 cut(s)	394
PdmI	GAANNNNTTC	1 cut(s)	432
PfeI	GAWTC	5 cut(s)	362, 583, 811, 1084, 1867
PleI	GAGTC	2 cut(s)	1131, 1205
PluTI	GGCGCC	1 cut(s)	849
PpsI	GAGTC	2 cut(s)	1131, 1205
PpuMI	RGGWCCY	1 cut(s)	511
PscI	ACATGT	1 cut(s)	1887
Psp124BI	GAGCTC	3 cut(s)	52, 292, 1437
Psp5II	RGGWCCY	1 cut(s)	511
Psp6I	CCWGG	4 cut(s)	57, 204, 1710, 1939
PspGI	CCWGG	4 cut(s)	57, 204, 1710, 1939
PspN4I	GGNNCC	8 cut(s)	512, 736, 847, 865, 989, 1011, 1803, 2431
PspOMI	GGGCCC	1 cut(s)	1801
PspPI	GGNCC	8 cut(s)	208, 511, 735, 1502, 1583, 1617, 1801, 1802
PspPPI	RGGWCCY	1 cut(s)	511
PstNI	CAGNNNCTG	2 cut(s)	211, 1363
PsuI	RGATCY	1 cut(s)	863
PvuII	CAGCTG	1 cut(s)	1255
RruI	TCGCGA	1 cut(s)	2295
RsaI	GTAC	5 cut(s)	920, 947, 1549, 2104, 2561
RsaNI	GTAC	5 cut(s)	919, 946, 1548, 2103, 2560
RseI	CAYNNNNRTG	4 cut(s)	275, 1074, 2262, 2366
SacI	GAGCTC	3 cut(s)	52, 292, 1437
SapI	GCTCTTC	1 cut(s)	1425
SaqAI	TTAA	5 cut(s)	767, 1416, 1664, 1742, 1925
Sau3AI	GATC	9 cut(s)	121, 334, 342, 863, 1066, 1165, 2296, 2416, 2625
Sau96I	GGNCC	8 cut(s)	208, 511, 735, 1502, 1583, 1617, 1801, 1802
ScaI	AGTACT	1 cut(s)	1549
SchI	GAGTC	2 cut(s)	1132, 1206
ScrFI	CCNGG	7 cut(s)	59, 206, 529, 542, 630, 1712, 1941
SduI	GDGCHC	5 cut(s)	52, 292, 1437, 1805, 2189
SfoI	GGCGCC	1 cut(s)	847
SfuI	TTCGAA	1 cut(s)	1408
SinI	GGWCC	3 cut(s)	511, 1583, 1617
SmiMI	CAYNNNNRTG	4 cut(s)	275, 1074, 2262, 2366
SmlI	CTYRAG	5 cut(s)	356, 938, 1277, 1415, 1492
SmoI	CTYRAG	5 cut(s)	356, 938, 1277, 1415, 1492
Sse9I	AATT	8 cut(s)	618, 697, 828, 1029, 1593, 1665, 1881, 1926
SseBI	AGGCCT	1 cut(s)	1996
SspDI	GGCGCC	1 cut(s)	845
SspMI	CTAG	6 cut(s)	254, 1014, 1169, 1299, 1479, 1760
SstI	GAGCTC	3 cut(s)	52, 292, 1437
StuI	AGGCCT	1 cut(s)	1996
StyD4I	CCNGG	7 cut(s)	57, 204, 527, 540, 628, 1710, 1939
StyI	CCWWGG	2 cut(s)	63, 867
TaaI	ACNGT	7 cut(s)	1076, 1579, 2003, 2107, 2125, 2429, 2521
TasI	AATT	8 cut(s)	618, 697, 828, 1029, 1593, 1665, 1881, 1926
TatI	WGTACW	3 cut(s)	945, 1547, 2559
TauI	GCSGC	1 cut(s)	2597
TfiI	GAWTC	5 cut(s)	362, 583, 811, 1084, 1867
Tru1I	TTAA	5 cut(s)	767, 1416, 1664, 1742, 1925
Tru9I	TTAA	5 cut(s)	767, 1416, 1664, 1742, 1925
TscAI	CASTG	5 cut(s)	133, 172, 1079, 1368, 2008
TseFI	GTSAC	5 cut(s)	231, 374, 554, 1630, 2374
TseI	GCWGC	9 cut(s)	971, 1109, 1252, 1484, 2042, 2248, 2339, 2384, 2534
Tsp45I	GTSAC	5 cut(s)	231, 374, 554, 1630, 2374
TspGWI	ACGGA	3 cut(s)	872, 1991, 2592
TspRI	CASTG	5 cut(s)	133, 172, 1079, 1368, 2008
Vha464I	CTTAAG	1 cut(s)	1415
VpaK11BI	GGWCC	3 cut(s)	511, 1583, 1617
XapI	RAATTY	1 cut(s)	828
XceI	RCATGY	1 cut(s)	1891
XmiI	GTMKAC	1 cut(s)	960
XmnI	GAANNNNTTC	1 cut(s)	432
XspI	CTAG	6 cut(s)	254, 1014, 1169, 1299, 1479, 1760
ZrmI	AGTACT	1 cut(s)	1549

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.