Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0002819

Physical Location & Seq

Genomic Coordinates Forward (+)

33480057 .. 33484274

Gene Structure 4218 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1201600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2739 bp

ATGAATCCTGATAGCTTTACTCACAAGACCAATGAGACTCTTGCCGGAGCTCATGGCCTGGCCATGGAATCCGGCCACGCTCAGATCACGCCGCTCCATCTTGCAACCGCGTTGGCATCTGATCCCAGCGGCATTTTCAGCCAAGCCATCGCCAATGGCGCTGGCAATCCCGATGCTCCAAAGTCCGTAGAGAGGGTGTTCAACCAGGCCCTGAAGAAGCTTCCATCTCAATCACCTCCGCCGGACGAAATCCCAGCCAGTTCATCCCTCATCAAAGTGATTAGGAGAGCACAGAAAGCGCAGAAGACAAGAGGGGACACACACCTGGCCGTTGATCAGTTGATTCTCGGTCTGCTCGAGGATTCCCAGATTGGGGACTTGCTGAAGGAAGCCAGCGTTGCTACGGCCAAAGTGAAATCTGAAGTCGAAAAGCTTCGTGGGAAGGAAGGGAAGAAGGTGGAGAGTGCTTCAGGGGATACTACATTTCAGGCATTGAAGACTTATGGACGGGACCTTGTTGAGCAGGCTGGGAAGCTTGACCCGGTTATTGGCCGTGATGAGGAGATAAGGAGGGTGGTGAGGATTCTTTCGAGGAGGACTAAGAACAATCCGGTTCTGATCGGAGAGCCGGGAGTTGGAAAAACTGCTGTGGTGGAAGGACTGGCTCAGAGGATAGTCGGTGGTGATGTCCCTAGTAATCTGGCTGATGTGAGGCTCATTGCATTGGATATGGGAGCTTTGGTGGCCGGGGCTAAGTATCGAGGGGAGTTCGAGGAGCGGTTGAAGGCAGTTTTGAAGGAAGTGGAAGAGGCTGAGGGGAAGGTGATTCTGTTTATTGATGAAATTCACCTTGTTCTTGGCGCTGGAAGGACAGAGGGGTCTATGGATGCAGCCAACCTGTTCAAGCCAATGCTCGCACGGGGGCAGCTGAGGTGCATTGGCGCAACAACTCTTGAGGAGTACAGAAAATATGTGGAGAAGGATGCTGCATTTGAGAGAAGGTTCCAGCAGGTTTACGTGGCGGAGCCTAGTGTTCCGGACACAATTAGCATCCTTCGAGGACTGAAAGAGAGGTATGAGGGTCACCACGGTGTTCGAATTCTGGACAGAGCTCTTGTTGTTGCTGCACAGCTTTCTAGCCGGTACATCACAGGGCGACATCTCCCTGACAAGGCGATTGATCTAGTTGACGAAGCTTGTGCGAATGTGAGAGTCCAACTTGACAGCCAGCCTGAAGAAATTGATAACCTCGAAAGGAAGAGGATGCAGCTGGAAGTTGAGCTTCACGCACTTGAGAAAGAGAAGGACAAAGCTAGCAAAGCTCGTCTTGTCGAGGTGCGAAAAGAGCTCGATGAACTGCGAGACAAGCTACAGCCGCTGGTGATGAAGTATAGAAAGGAGAAGGAAAGGGTTGATGAGCTTCGAAAGCTTAAGCAGAAGCGTGAAGAGCTCTTGGTAGCGTTAGCAGAGGCCGAAAGAAGATACGATTTAGCTAGGGCCGCAGACTTGCGTTATGGCGCTATTCAGGAGGTGGAAACCACCATTGCAAAACTTGAAGGTAGTACCGACGAGAACTTGATTTTGACGGAGACGGTTGGACCAGAACAAATTGCAGAGGTAGTAAGCCGTTGGACTGGCATACCTGTCACGCGGCTTGGCCAGAATGACAAAGAAAGGTTAATTGGACTTGGGGAAAGGTTGCATAAGAGAGTAGTGGGGCAAGAGAGGGCAGTTGAAGCTGTCGCCGAGGCGGTTTTAAGGTCAAGAGCGGGGCTAGGAAGGCCACAACAGCCTACCGGCTCCTTCCTGTTCTTGGGTCCAACTGGTGTTGGTAAAACTGAGCTTGCGAAGGCGCTTGCTGAACAACTTTTTGATGACGAAAATCTGATCGTCAGAATTGACATGTCCGAGTATATGGAACAACATTCCGTTTCGCGCTTAATTGGCGCTCCCCCTGGGTATGTTGGTCACGATGAAGGCGGGCAACTCACAGAGGCCGTGAGGCGGAGGCCTTACAGCGTTGTGTTGTTTGATGAAGTGGAGAAGGCCCACACCGCCGTTTTCAACACTCTCCTCCAAGTCCTGGATGATGGAAGACTAACTGATGGACAAGGCCGTACAGTTGATTTCAGAAACACGGTGATCATCATGACTTCGAATTTGGGGGCAGAACATCTCCTCTCCGGTTTGATGGGCAAGTGTTCGATGCAGGTTGCGCGTGATCGAGTTATGCAAGAGGTGAGAAAGCACTTCAGGCCTGAGCTGCTGAATCGTCTCGATGAGATTGTTGTGTTCGATCCTCTTTCTCGCGATCAACTGAGGAAGGTTGCCAGACTGCAAATGAAAGATGTTGCAGTCCGACTGGCTGAGAGGGGCGTCGCGATGGCAGTTACAGACGGAGCATTGGATTACATTCTAGACGAGAGTTATGATCCCGTTTACGGTGCTAGACCTATTCGGAGATGGCTCGAGAAGAGGGTGGTGACGGAGTTGTCGCGGATGCTTGTGCGAGAAGAAATAGATGAAAACTCGACCGTGTTCGTAGATGTAGCGCCAACGGGAACTGGGCTGCTGTACCGGGTTGAGAAGAACGGAGGGCTTGTCAATGCGGCGACAGGGCAGAAGTCGGATGTGTTGATCCAGGTCTCGAATGCCAACGGGCCAAGGTCTGATGCAGAACAGGCTGTTAAGAAGATGAAGATTGAGGAAATTGATGAAGATGTTGACATGGATGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006417 GO:0006950 GO:0006979 GO:0008150 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009636 GO:0009642 GO:0009644 GO:0009889 GO:0009891 GO:0009893 GO:0009941 GO:0009987 GO:0010035 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031967 GO:0031975 GO:0032268 GO:0032270 GO:0033554 GO:0034248 GO:0034250 GO:0034605 GO:0042221 GO:0042493 GO:0042542 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043335 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045727 GO:0046677 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051716 GO:0060255 GO:0065007 GO:0080090 GO:1901700 GO:2000112

KEGG Pathways

Metabolic & Signaling

ko04213 map04213

Pfam Domains

Protein Families

AAA AAA_2 ClpB_D2-small Clp_N

Protein Analysis

913

Amino Acids

101.05

Weight (kDa)

5.87

Isoelectric Point (pI)

34.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Clp_N	PF02861	6 - 130	1.1e-27	Clp repeat (R) N-terminal domain
AAA_16	PF13191	182 - 228	2e-06	AAA ATPase domain
NBD_SMAX1	PF23569	186 - 288	2.2e-08	SMAX1 nucleotide binding domain
AAA	PF00004	205 - 318	3.6e-13	ATPase family associated with various cellular activities (AAA)
AAA_lid_9	PF17871	344 - 445	2.8e-34	AAA lid domain
AAA_2	PF07724	598 - 762	5.6e-69	AAA domain (Cdc48 subfamily)
Sigma54_activat	PF00158	601 - 718	5.3e-06	Sigma-54 interaction domain
AAA_5	PF07728	602 - 721	5.7e-12	AAA domain (dynein-related subfamily)
AAA	PF00004	603 - 738	1.9e-10	ATPase family associated with various cellular activities (AAA)
ClpB_D2-small	PF10431	768 - 847	1.1e-21	C-terminal, D2-small domain, of ClpB protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0002819)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74310 AT1G74310 AT4G14670
fragaria_vesca	FvH4_5g14020 FvH4_5g14020 FvH4_5g14020 FvH4_5g14020 FvH4_7g22310 FvH4_7g22310 FvH4_7g22310
malus_domestica	MD06G1201600.v1.1 MD14G1210600.v1.1 MD14G1211000.v1.1
prunus_persica	Prupe.5G203700_v2.0.a1
pyrus_communis	pycom06g17750 pycom14g17400
rosa_chinensis	RchiOBHm_Chr1g0366791 RchiOBHm_Chr7g0182871 RchiOBHm_Chr7g0182901
rosa_laevigata	RLG00000005096 RLG00000005098 RLG00000027319
rosa_multiflora	Rmu_sc0000014.1_g000026 Rmu_sc0000318.1_g000026
rosa_roxburghii	Rroxscaffold_3G00230950 Rroxscaffold_3G00270960
rosa_rugosa	Rorug01G0331900 Rorug01G0333200 Rorug06G0451000
rosa_samantha	Rh1AG340000 Rh7AG054200 Rh7AG403300 Rh7BG053700 Rh7CG055300 Rh7DG053500 Rh7DG053600
rosa_wichuraiana	Rw0G008600 Rw1G030200 Rw7G004420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	877, 2491
Acc36I	ACCTGC	2 cut(s)	1000, 2200
AccB7I	CCANNNNNTGG	1 cut(s)	2083
AccBSI	CCGCTC	3 cut(s)	94, 778, 1769
AccII	CGCG	7 cut(s)	110, 1651, 1936, 2218, 2310, 2381, 2497
AccIII	TCCGGA	1 cut(s)	1036
AclWI	GGATC	4 cut(s)	116, 2291, 2426, 2632
AcoI	YGGCCR	7 cut(s)	60, 73, 327, 405, 550, 744, 1657
AcsI	RAATTY	3 cut(s)	843, 1098, 2158
AcuI	CTGAAG	6 cut(s)	233, 404, 441, 453, 1254, 2236
AcyI	GRCGYC	1 cut(s)	2376
AfaI	GTAC	5 cut(s)	962, 1145, 1564, 2119, 2576
AflII	CTTAAG	1 cut(s)	1430
AflIII	ACRYGT	1 cut(s)	1902
AgsI	TTSAA	8 cut(s)	202, 496, 784, 796, 904, 1556, 1736, 2065
AjnI	CCWGG	6 cut(s)	57, 204, 324, 1954, 2082, 2640
AjuI	GAANNNNNNNTTGG	2 cut(s)	1665, 1697
AleI	CACNNNNGTG	1 cut(s)	1089
Alw21I	GWGCWC	5 cut(s)	52, 292, 1114, 1350, 1452
Alw26I	GTCTC	5 cut(s)	29, 1356, 1583, 2279, 2650
AlwI	GGATC	4 cut(s)	116, 2291, 2426, 2632
AlwNI	CAGNNNCTG	2 cut(s)	211, 1378
Ama87I	CYCGRG	2 cut(s)	356, 2468
Aor13HI	TCCGGA	1 cut(s)	1036
ApeKI	GCWGC	7 cut(s)	890, 925, 986, 1124, 1267, 2263, 2569
ApoI	RAATTY	3 cut(s)	843, 1098, 2158
ArsI	GACNNNNNNTTYG	2 cut(s)	2143, 2175
Asp700I	GAANNNNTTC	1 cut(s)	432
AspS9I	GGNCC	7 cut(s)	208, 511, 1497, 1598, 1817, 2047, 2660
AsuC2I	CCSGG	4 cut(s)	542, 630, 748, 2579
AsuII	TTCGAA	3 cut(s)	1096, 1423, 2156
AsuNHI	GCTAGC	1 cut(s)	1313
AvaI	CYCGRG	2 cut(s)	356, 2468
AvaII	GGWCC	3 cut(s)	511, 1598, 1817
BalI	TGGCCA	2 cut(s)	62, 1659
BanII	GRGCYC	4 cut(s)	52, 1114, 1350, 1452
BbsI	GAAGAC	3 cut(s)	311, 503, 2101
Bbv12I	GWGCWC	5 cut(s)	52, 292, 1114, 1350, 1452
BbvCI	CCTCAGC	2 cut(s)	813, 929
BbvI	GCAGC	7 cut(s)	902, 937, 973, 1111, 1279, 2250, 2556
BccI	CCATC	8 cut(s)	105, 155, 232, 2084, 2099, 2185, 2377, 2457
BceAI	ACGGC	7 cut(s)	314, 420, 537, 1611, 1982, 2042, 2100
BcgI	CGANNNNNNTGC	2 cut(s)	1181, 1215
BciT130I	CCWGG	6 cut(s)	59, 206, 326, 1956, 2084, 2642
BciVI	GTATCC	1 cut(s)	469
BclI	TGATCA	2 cut(s)	334, 2142
BcnI	CCSGG	4 cut(s)	542, 630, 748, 2579
BcoDI	GTCTC	5 cut(s)	29, 1356, 1583, 2279, 2650
BfaI	CTAG	9 cut(s)	693, 1029, 1137, 1184, 1314, 1494, 1775, 2417, 2448
BfmI	CTRYAG	1 cut(s)	1370
BfoI	RGCGCY	6 cut(s)	162, 864, 1521, 1856, 1950, 2555
BfrI	CTTAAG	1 cut(s)	1430
BfuAI	ACCTGC	2 cut(s)	1000, 2200
BfuI	GTATCC	1 cut(s)	469
Bme18I	GGWCC	3 cut(s)	511, 1598, 1817
BmeT110I	CYCGRG	2 cut(s)	356, 2468
BmgT120I	GGNCC	7 cut(s)	208, 511, 1497, 1598, 1817, 2047, 2660
BmiI	GGNNCC	5 cut(s)	512, 1004, 1026, 1801, 1818
BmrI	ACTGGG	1 cut(s)	2574
BmsI	GCATC	9 cut(s)	125, 163, 877, 973, 1059, 1254, 2196, 2490, 2662
BmtI	GCTAGC	1 cut(s)	1317
BmuI	ACTGGG	1 cut(s)	2574
BpiI	GAAGAC	3 cut(s)	311, 503, 2101
Bpu10I	CCTNAGC	3 cut(s)	813, 929, 2259
Bpu14I	TTCGAA	3 cut(s)	1096, 1423, 2156
BpuEI	CTTGAG	2 cut(s)	974, 1313
BpuMI	CCSGG	4 cut(s)	542, 630, 748, 2579
BsaAI	YACGTR	1 cut(s)	1018
BsaHI	GRCGYC	1 cut(s)	2376
BsaI	GGTCTC	1 cut(s)	2650
BsaJI	CCNNGG	7 cut(s)	63, 747, 1087, 1746, 1954, 1955, 2663
BsaWI	WCCGGW	3 cut(s)	610, 1036, 2183
BsaXI	ACNNNNNCTCC	4 cut(s)	726, 756, 2586, 2616
Bse118I	RCCGGY	2 cut(s)	1140, 1796
Bse1I	ACTGG	6 cut(s)	258, 666, 1639, 1828, 2367, 2569
Bse3DI	GCAATG	2 cut(s)	717, 1542
BseAI	TCCGGA	1 cut(s)	1036
BseBI	CCWGG	6 cut(s)	59, 206, 326, 1956, 2084, 2642
BseDI	CCNNGG	7 cut(s)	63, 747, 1087, 1746, 1954, 1955, 2663
BseGI	GGATG	9 cut(s)	263, 892, 988, 1050, 1269, 2092, 2505, 2635, 2737
BseMI	GCAATG	2 cut(s)	717, 1542
BseMII	CTCAG	8 cut(s)	95, 680, 804, 920, 1830, 2250, 2309, 2358
BseNI	ACTGG	6 cut(s)	258, 666, 1639, 1828, 2367, 2569
BseRI	GAGGAG	6 cut(s)	575, 607, 788, 971, 2063, 2168
BseXI	GCAGC	7 cut(s)	902, 937, 973, 1111, 1279, 2250, 2556
BseYI	CCCAGC	3 cut(s)	125, 253, 527
BsgI	GTGCAG	1 cut(s)	1110
Bsh1236I	CGCG	7 cut(s)	110, 1651, 1936, 2218, 2310, 2381, 2497
Bsh1285I	CGRYCG	1 cut(s)	2535
BsiEI	CGRYCG	1 cut(s)	2535
BsiHKAI	GWGCWC	5 cut(s)	52, 292, 1114, 1350, 1452
BsiHKCI	CYCGRG	2 cut(s)	356, 2468
BslFI	GGGAC	4 cut(s)	329, 389, 524, 674
BsmAI	GTCTC	5 cut(s)	29, 1356, 1583, 2279, 2650
BsmBI	CGTCTC	2 cut(s)	1583, 2279
BsmFI	GGGAC	4 cut(s)	329, 389, 524, 674
BsmI	GAATGC	1 cut(s)	2656
Bso31I	GGTCTC	1 cut(s)	2650
BsoBI	CYCGRG	2 cut(s)	356, 2468
Bsp119I	TTCGAA	3 cut(s)	1096, 1423, 2156
Bsp1286I	GDGCHC	5 cut(s)	52, 292, 1114, 1350, 1452
Bsp13I	TCCGGA	1 cut(s)	1036
Bsp19I	CCATGG	1 cut(s)	63
Bsp68I	TCGCGA	2 cut(s)	2310, 2381
BspCNI	CTCAG	8 cut(s)	94, 679, 805, 921, 1831, 2251, 2310, 2359
BspEI	TCCGGA	1 cut(s)	1036
BspFNI	CGCG	7 cut(s)	110, 1651, 1936, 2218, 2310, 2381, 2497
BspHI	TCATGA	1 cut(s)	2148
BspLI	GGNNCC	5 cut(s)	512, 1004, 1026, 1801, 1818
BspMI	ACCTGC	2 cut(s)	1000, 2200
BspOI	GCTAGC	1 cut(s)	1317
BspPI	GGATC	4 cut(s)	116, 2291, 2426, 2632
BspQI	GCTCTTC	1 cut(s)	1440
BspT104I	TTCGAA	3 cut(s)	1096, 1423, 2156
BspTI	CTTAAG	1 cut(s)	1430
BspTNI	GGTCTC	1 cut(s)	2650
BsrBI	CCGCTC	3 cut(s)	94, 778, 1769
BsrDI	GCAATG	2 cut(s)	717, 1542
BsrFI	RCCGGY	2 cut(s)	1140, 1796
BsrI	ACTGG	6 cut(s)	258, 666, 1639, 1828, 2367, 2569
BssAI	RCCGGY	2 cut(s)	1140, 1796
BssECI	CCNNGG	7 cut(s)	63, 747, 1087, 1746, 1954, 1955, 2663
BssNI	GRCGYC	1 cut(s)	2376
BssT1I	CCWWGG	2 cut(s)	63, 2663
Bst2UI	CCWGG	6 cut(s)	59, 206, 326, 1956, 2084, 2642
Bst4CI	ACNGT	6 cut(s)	1091, 1594, 2122, 2140, 2444, 2536
Bst6I	CTCTTC	4 cut(s)	801, 1253, 1440, 2468
BstACI	GRCGYC	1 cut(s)	2376
BstAFI	CTTAAG	1 cut(s)	1430
BstBAI	YACGTR	1 cut(s)	1018
BstBI	TTCGAA	3 cut(s)	1096, 1423, 2156
BstC8I	GCNNGC	9 cut(s)	163, 394, 525, 915, 1229, 1315, 1845, 1857, 1982
BstDSI	CCRYGG	2 cut(s)	63, 1087
BstEII	GGTNACC	1 cut(s)	1082
BstF5I	GGATG	9 cut(s)	263, 892, 988, 1050, 1269, 2092, 2505, 2635, 2737
BstFNI	CGCG	7 cut(s)	110, 1651, 1936, 2218, 2310, 2381, 2497
BstH2I	RGCGCY	6 cut(s)	162, 864, 1521, 1856, 1950, 2555
BstMAI	GTCTC	5 cut(s)	29, 1356, 1583, 2279, 2650
BstMCI	CGRYCG	1 cut(s)	2535
BstNI	CCWGG	6 cut(s)	59, 206, 326, 1956, 2084, 2642
BstNSI	RCATGY	1 cut(s)	1906
BstPI	GGTNACC	1 cut(s)	1082
BstSFI	CTRYAG	1 cut(s)	1370
BstUI	CGCG	7 cut(s)	110, 1651, 1936, 2218, 2310, 2381, 2497
BstV1I	GCAGC	7 cut(s)	902, 937, 973, 1111, 1279, 2250, 2556
BstV2I	GAAGAC	3 cut(s)	311, 503, 2101
BsuI	GTATCC	1 cut(s)	469
BtgI	CCRYGG	2 cut(s)	63, 1087
BtgZI	GCGATG	2 cut(s)	133, 2396
BtsCI	GGATG	9 cut(s)	263, 892, 988, 1050, 1269, 2092, 2505, 2635, 2737
BtuMI	TCGCGA	2 cut(s)	2310, 2381
BveI	ACCTGC	2 cut(s)	1000, 2200
Cac8I	GCNNGC	9 cut(s)	163, 394, 525, 915, 1229, 1315, 1845, 1857, 1982
CaiI	CAGNNNCTG	2 cut(s)	211, 1378
CciI	TCATGA	1 cut(s)	2148
Cfr10I	RCCGGY	2 cut(s)	1140, 1796
Cfr13I	GGNCC	7 cut(s)	208, 511, 1497, 1598, 1817, 2047, 2660
CseI	GACGC	1 cut(s)	2365
Csp6I	GTAC	5 cut(s)	961, 1144, 1563, 2118, 2575
CviAII	CATG	5 cut(s)	53, 64, 1903, 2149, 2728
CviQI	GTAC	5 cut(s)	961, 1144, 1563, 2118, 2575
DrdI	GACNNNNNNGTC	2 cut(s)	877, 2491
DseDI	GACNNNNNNGTC	2 cut(s)	877, 2491
EaeI	YGGCCR	7 cut(s)	60, 73, 327, 405, 550, 744, 1657
Eam1104I	CTCTTC	4 cut(s)	801, 1253, 1440, 2468
EarI	CTCTTC	4 cut(s)	801, 1253, 1440, 2468
EciI	GGCGGA	3 cut(s)	228, 1037, 2020
Ecl136II	GAGCTC	4 cut(s)	50, 1112, 1348, 1450
Eco130I	CCWWGG	2 cut(s)	63, 2663
Eco147I	AGGCCT	2 cut(s)	2011, 2257
Eco24I	GRGCYC	4 cut(s)	52, 1114, 1350, 1452
Eco31I	GGTCTC	1 cut(s)	2650
Eco47I	GGWCC	3 cut(s)	511, 1598, 1817
Eco53kI	GAGCTC	4 cut(s)	50, 1112, 1348, 1450
Eco57I	CTGAAG	6 cut(s)	233, 404, 441, 453, 1254, 2236
Eco88I	CYCGRG	2 cut(s)	356, 2468
Eco91I	GGTNACC	1 cut(s)	1082
EcoICRI	GAGCTC	4 cut(s)	50, 1112, 1348, 1450
EcoO109I	RGGNCCY	2 cut(s)	208, 511
EcoO65I	GGTNACC	1 cut(s)	1082
EcoRI	GAATTC	1 cut(s)	1098
EcoRII	CCWGG	6 cut(s)	57, 204, 324, 1954, 2082, 2640
EcoT14I	CCWWGG	2 cut(s)	63, 2663
EcoT38I	GRGCYC	4 cut(s)	52, 1114, 1350, 1452
ErhI	CCWWGG	2 cut(s)	63, 2663
Esp3I	CGTCTC	2 cut(s)	1583, 2279
FaeI	CATG	5 cut(s)	56, 67, 1906, 2152, 2731
FalI	AAGNNNNNCTT	4 cut(s)	1266, 1298, 1311, 1343
FaqI	GGGAC	4 cut(s)	329, 389, 524, 674
FatI	CATG	5 cut(s)	52, 63, 1902, 2148, 2727
FauI	CCCGC	2 cut(s)	1762, 1973
FbaI	TGATCA	2 cut(s)	334, 2142
FokI	GGATG	8 cut(s)	250, 899, 995, 1037, 1276, 2099, 2512, 2642
FriOI	GRGCYC	4 cut(s)	52, 1114, 1350, 1452
FspBI	CTAG	9 cut(s)	693, 1029, 1137, 1184, 1314, 1494, 1775, 2417, 2448
GsaI	CCCAGC	3 cut(s)	129, 257, 531
HaeII	RGCGCY	6 cut(s)	162, 864, 1521, 1856, 1950, 2555
HgaI	GACGC	1 cut(s)	2365
Hin1I	GRCGYC	1 cut(s)	2376
Hin1II	CATG	5 cut(s)	56, 67, 1906, 2152, 2731
HincII	GTYRAC	2 cut(s)	1189, 2725
HindII	GTYRAC	2 cut(s)	1189, 2725
HindIII	AAGCTT	5 cut(s)	218, 431, 533, 1194, 1427
HinfI	GANTC	9 cut(s)	4, 37, 68, 343, 362, 583, 826, 1212, 2269
Hpy166II	GTNNAC	4 cut(s)	1015, 1189, 2440, 2725
Hpy8I	GTNNAC	4 cut(s)	1015, 1189, 2440, 2725
Hpy99I	CGWCG	2 cut(s)	1571, 2381
HpyCH4III	ACNGT	6 cut(s)	1091, 1594, 2122, 2140, 2444, 2536
HpyCH4IV	ACGT	1 cut(s)	1017
HpySE526I	ACGT	1 cut(s)	1017
Hsp92I	GRCGYC	1 cut(s)	2376
Hsp92II	CATG	5 cut(s)	56, 67, 1906, 2152, 2731
Kpn2I	TCCGGA	1 cut(s)	1036
Ksp22I	TGATCA	2 cut(s)	334, 2142
LguI	GCTCTTC	1 cut(s)	1440
LmnI	GCTCC	9 cut(s)	47, 99, 181, 734, 775, 1024, 1805, 1954, 2399
Lsp1109I	GCAGC	7 cut(s)	902, 937, 973, 1111, 1279, 2250, 2556
LweI	GCATC	9 cut(s)	125, 163, 877, 973, 1059, 1254, 2196, 2490, 2662
MaeI	CTAG	9 cut(s)	693, 1029, 1137, 1184, 1314, 1494, 1775, 2417, 2448
MaeII	ACGT	1 cut(s)	1017
MaeIII	GTNAC	5 cut(s)	1082, 1645, 1967, 2389, 2482
MbiI	CCGCTC	3 cut(s)	94, 778, 1769
MhlI	GDGCHC	5 cut(s)	52, 292, 1114, 1350, 1452
MlsI	TGGCCA	2 cut(s)	62, 1659
MluNI	TGGCCA	2 cut(s)	62, 1659
MlyI	GAGTC	2 cut(s)	31, 1221
MmeI	TCCRAC	7 cut(s)	616, 1240, 1576, 1610, 1844, 2383, 2607
Mox20I	TGGCCA	2 cut(s)	62, 1659
MroI	TCCGGA	1 cut(s)	1036
MroXI	GAANNNNTTC	1 cut(s)	432
MscI	TGGCCA	2 cut(s)	62, 1659
MseI	TTAA	5 cut(s)	1431, 1679, 1757, 1940, 2688
MslI	CAYNNNNRTG	2 cut(s)	275, 1089
Msp20I	TGGCCA	2 cut(s)	62, 1659
MspA1I	CMGCKG	4 cut(s)	129, 928, 1270, 1378
MspCI	CTTAAG	1 cut(s)	1430
Mva1269I	GAATGC	1 cut(s)	2656
MvaI	CCWGG	6 cut(s)	59, 206, 326, 1956, 2084, 2642
MvnI	CGCG	7 cut(s)	110, 1651, 1936, 2218, 2310, 2381, 2497
NciI	CCSGG	4 cut(s)	542, 630, 748, 2579
NcoI	CCATGG	1 cut(s)	63
NheI	GCTAGC	1 cut(s)	1313
NlaIII	CATG	5 cut(s)	56, 67, 1906, 2152, 2731
NlaIV	GGNNCC	5 cut(s)	512, 1004, 1026, 1801, 1818
NmeAIII	GCCGAG	1 cut(s)	1771
NmuCI	GTSAC	4 cut(s)	1082, 1645, 1967, 2482
NruI	TCGCGA	2 cut(s)	2310, 2381
NspI	RCATGY	1 cut(s)	1906
NspV	TTCGAA	3 cut(s)	1096, 1423, 2156
OliI	CACNNNNGTG	1 cut(s)	1089
PaeR7I	CTCGAG	2 cut(s)	356, 2468
PagI	TCATGA	1 cut(s)	2148
PasI	CCCWGGG	1 cut(s)	1955
PceI	AGGCCT	2 cut(s)	2011, 2257
PciI	ACATGT	1 cut(s)	1902
PciSI	GCTCTTC	1 cut(s)	1440
PcsI	WCGNNNNNNNCGW	1 cut(s)	354
PctI	GAATGC	1 cut(s)	2656
PdmI	GAANNNNTTC	1 cut(s)	432
PfeI	GAWTC	7 cut(s)	4, 68, 343, 362, 583, 826, 2269
PflMI	CCANNNNNTGG	1 cut(s)	2083
PfoI	TCCNGGA	1 cut(s)	2082
PleI	GAGTC	2 cut(s)	31, 1220
PpsI	GAGTC	2 cut(s)	31, 1220
Ppu21I	YACGTR	1 cut(s)	1018
PpuMI	RGGWCCY	1 cut(s)	511
PscI	ACATGT	1 cut(s)	1902
Psp124BI	GAGCTC	4 cut(s)	52, 1114, 1350, 1452
Psp5II	RGGWCCY	1 cut(s)	511
Psp6I	CCWGG	6 cut(s)	57, 204, 324, 1954, 2082, 2640
PspEI	GGTNACC	1 cut(s)	1082
PspFI	CCCAGC	3 cut(s)	125, 253, 527
PspGI	CCWGG	6 cut(s)	57, 204, 324, 1954, 2082, 2640
PspN4I	GGNNCC	5 cut(s)	512, 1004, 1026, 1801, 1818
PspPI	GGNCC	7 cut(s)	208, 511, 1497, 1598, 1817, 2047, 2660
PspPPI	RGGWCCY	1 cut(s)	511
PspXI	VCTCGAGB	1 cut(s)	356
PstNI	CAGNNNCTG	2 cut(s)	211, 1378
PvuII	CAGCTG	2 cut(s)	928, 1270
RruI	TCGCGA	2 cut(s)	2310, 2381
RsaI	GTAC	5 cut(s)	962, 1145, 1564, 2119, 2576
RsaNI	GTAC	5 cut(s)	961, 1144, 1563, 2118, 2575
RseI	CAYNNNNRTG	2 cut(s)	275, 1089
SacI	GAGCTC	4 cut(s)	52, 1114, 1350, 1452
SapI	GCTCTTC	1 cut(s)	1440
SaqAI	TTAA	5 cut(s)	1431, 1679, 1757, 1940, 2688
Sau96I	GGNCC	7 cut(s)	208, 511, 1497, 1598, 1817, 2047, 2660
SchI	GAGTC	2 cut(s)	31, 1221
SduI	GDGCHC	5 cut(s)	52, 292, 1114, 1350, 1452
SfaNI	GCATC	9 cut(s)	125, 163, 877, 973, 1059, 1254, 2196, 2490, 2662
SfcI	CTRYAG	1 cut(s)	1370
Sfr274I	CTCGAG	2 cut(s)	356, 2468
SfuI	TTCGAA	3 cut(s)	1096, 1423, 2156
SinI	GGWCC	3 cut(s)	511, 1598, 1817
SlaI	CTCGAG	2 cut(s)	356, 2468
SmiMI	CAYNNNNRTG	2 cut(s)	275, 1089
SmlI	CTYRAG	5 cut(s)	356, 953, 1292, 1430, 2468
SmoI	CTYRAG	5 cut(s)	356, 953, 1292, 1430, 2468
SseBI	AGGCCT	2 cut(s)	2011, 2257
SspMI	CTAG	9 cut(s)	693, 1029, 1137, 1184, 1314, 1494, 1775, 2417, 2448
SstI	GAGCTC	4 cut(s)	52, 1114, 1350, 1452
StuI	AGGCCT	2 cut(s)	2011, 2257
StyI	CCWWGG	2 cut(s)	63, 2663
TaaI	ACNGT	6 cut(s)	1091, 1594, 2122, 2140, 2444, 2536
TaiI	ACGT	1 cut(s)	1020
TaqII	GACCGA	1 cut(s)	338
TatI	WGTACW	1 cut(s)	960
TauI	GCSGC	6 cut(s)	94, 132, 1378, 1502, 1654, 2612
TfiI	GAWTC	7 cut(s)	4, 68, 343, 362, 583, 826, 2269
Tru1I	TTAA	5 cut(s)	1431, 1679, 1757, 1940, 2688
Tru9I	TTAA	5 cut(s)	1431, 1679, 1757, 1940, 2688
TseFI	GTSAC	4 cut(s)	1082, 1645, 1967, 2482
TseI	GCWGC	7 cut(s)	890, 925, 986, 1124, 1267, 2263, 2569
Tsp45I	GTSAC	4 cut(s)	1082, 1645, 1967, 2482
TspGWI	ACGGA	6 cut(s)	175, 1601, 1918, 2412, 2501, 2607
Van91I	CCANNNNNTGG	1 cut(s)	2083
Vha464I	CTTAAG	1 cut(s)	1430
VpaK11BI	GGWCC	3 cut(s)	511, 1598, 1817
XapI	RAATTY	3 cut(s)	843, 1098, 2158
XbaI	TCTAGA	1 cut(s)	2416
XceI	RCATGY	1 cut(s)	1906
XhoI	CTCGAG	2 cut(s)	356, 2468
XmnI	GAANNNNTTC	1 cut(s)	432
XspI	CTAG	9 cut(s)	693, 1029, 1137, 1184, 1314, 1494, 1775, 2417, 2448

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.