Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0002819

Physical Location & Seq

Genomic Coordinates Reverse (-)

7937467 .. 7941463

Gene Structure 3997 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g14020.t4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2628 bp

ATGAATCCTGACAAGTTCACACACAAGACCAATGAGTCTCTGGCCGCCGCTCATGAACTCGCAAGCGACGCCGGCCACGTGCAGTTCACGCCGCTCCATCTCGCCACCGCTTTGATCTCCGACCCCGCCGGCATTTTCCGTCAGGCGATTGCCAATGCCGGCGGCAGCGAGGAGTCTCCGAAGTCGGTGGAGAGAGTTTTCAACCAGGCGCTGAAGAAGCTTCCGTCGCAGTCGCCTCCGCCGGAGCAAGTACCGGCGAGTACTACTCTGATTAAGGTGATTCGGAGGGCGCAGTCGGCGCAGAAGTCCCGAGGGGACACGCATTTGGCTGTTGATCAGTTGATTCTGGGGCTTTTGGAGGACTCGCAGATTGGGGATTTGATGAAGGAGGCTGGCATTGCGGCGGCGAAGGTGAAATCGGAGCTTGATCCTGTGATAGGCAGAGATGAGGAGATAAGGAGGGTGGTGAGGATTCTTTCGCGGAGGACGAAGAATAATCCGGTGCTGATTGGAGAGCCTGGAGTTGGGAAGACTGCGGTGGTTGAGGGTTTGGCTCAGAGGATTGTAAGAGGGGATGTTCCTAGTAACTTGGCTGATGTCAGGCTGATTGCATTGGACATGGGTGCTCTCGTGGCGGGTGCAAAGTACAGAGGGGAGTTTGAGGAGAGGCTGAAGGCTGTGTTGAAAGAAGTAGAGGAGGCTGAAGGGAAGGTGATCCTGTTTATTGATGAAATCCACCTTGTTCTTGGTGCTGGTAGAACTGAAGGGTCTATGGATGCTGCAAATCTTTTCAAGCCGATGCTCGCTAGAGGTCAGCTCCGGTGCATTGGGGCAACGACTCTAGAGGAGTATAGGAAGTATGTGGAGAAAGATGCTGCGTTTGAGAGAAGGTTTCAGCAGGTTTACGTGGCGGAACCCAGTGTTCCGGATACGGTTAGCATCCTACGAGGCTTGAAGGAGAGATATGAAGGGCATCATGGTGTTCGAATTTTGGATAGAGCCTTGGTTATGGCTGCGCAACTGTCGAGTCGATACATCACAGGGCGCCATCTCCCTGACAAGGCAATTGATTTAGTTGATGAGGCTTGTGCGAATGTGAGAGTCCAGCTTGACAGTCAACCAGAAGAGATTGACAACCTTGAAAGAAAGAGAATGCAGCTGGAAGTTGAGCTTCATGCGCTTGAAAAGGAAAAGGATAAAGCTAGCAAAGCTCGGCTTGTTGAAGTTCGGAGAGAGCTTGATGACTTGAGAGACAAGCTCCAACCCTTGATGATGAAGTATAGAAAAGAGAAGGAGAGGATTGATGAGATTCGAAGGCTTAAGCAAAAGCGTGAGGAGCTCTTATTTGCTTTAACAGAGGCTGAAAGAAGATATGACCTAGCCAGGGCTGCTGATTTGCGTTACGGGGCTATTCAAGAAGTGGAATCTGCTATTGCTCAGCTTGAAGGAAGCACAGAGGAGAACTTGATGTTGACTGAAACTGTTGGACCAGAACACATTGCAGAGGTTGTAAGCCGTTGGACTGGTATACCAGTAACAAGGCTTGGCCAGGATGACAAACACAGGTTAATTGGACTTGGTGAGAGATTGCATAAGAGGGTGGTGGGTCAAAACCAGGCAGTGGATGCTGTGGCAGAAGCCGTGTTGAGATCAAGAGCTGGATTAGGCCGACCGCAGCAACCAACTGGATCATTTCTTTTCTTGGGTCCAACAGGTGTTGGTAAAACTGAGCTTGCAAAGGCCCTTGCTGAGCAACTTTTCGATGACGAAAATCTAATTGTCCGAATTGATATGTCTGAGTATATGGAGCAACATTCTGTTGCACGTCTGATTGGCGCTCCTCCTGGATATGTTGGTCATGAAGAAGGTGGACAACTCACGGAGGCTGTGAGGCGGAGGCCATATAGTGTTATATTGTTTGATGAAGTGGAGAAAGCTCACCCTACAGTTTTCAATACTCTCCTGCAAGTTCTAGATGATGGGCGATTAACTGATGGTCAAGGTCGTACAGTTGATTTTAGGAATACAGTGATCATTATGACTTCAAACCTTGGGGCGGAACATCTCCTAACTGGTTTACTGGGAAAGTGTTCAATGCAAGTTGCTCGTGATCGAGTTATGCAAGAGGTGAGGACGCACTTTAGGCCGGAACTGCTGAATCGGCTGGATGAGGTAGTTGTTTTCGATCCTCTTTCACGTGAGCAACTGAGGAAGGTAGCTAGGCTACAAATGAAGGATGTTGCTGCCCGACTTGCTGAGAGGGGTGTTGCTTTGGGTGTGACTGATAAAGCATTAGATTATATTCTGGATGAGAGTTATGATCCGGTTTATGGTGCTCGACCCATAAGAAGATGGCTTGAGAAGAGAGTGGTGACAGAGCTGTCAAGAATGCTGATAAGAGAAGAGATAGATGAGAACTCTACTGTGTACATAGACGCAGGGCCTAATGGAGGCGAGTTGGTTTACCGGGTTGAGAAGAATGGAGGCCTGGTTAATGCTGTCACCGGCCAGAAATCGGATGTGCTGATTCAACTACCGAATGGAGTAAGGTTGAACGATGATGCTCATCAGGCAGTTAAGAAGATGAAGATTGAGGAAATGGATGAGGACGAGGACATGGATGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006417 GO:0006950 GO:0006979 GO:0008150 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009636 GO:0009642 GO:0009644 GO:0009889 GO:0009891 GO:0009893 GO:0009941 GO:0009987 GO:0010035 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031967 GO:0031975 GO:0032268 GO:0032270 GO:0033554 GO:0034248 GO:0034250 GO:0034605 GO:0042221 GO:0042493 GO:0042542 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043335 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045727 GO:0046677 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051716 GO:0060255 GO:0065007 GO:0080090 GO:1901700 GO:2000112

KEGG Pathways

Metabolic & Signaling

ko04213 map04213

Pfam Domains

Protein Families

AAA AAA_2 ClpB_D2-small Clp_N

Protein Analysis

876

Amino Acids

97.65

Weight (kDa)

5.8

Isoelectric Point (pI)

40.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Clp_N	PF02861	5 - 130	3.3e-29	Clp repeat (R) N-terminal domain
AAA_16	PF13191	182 - 233	9.5e-07	AAA ATPase domain
NBD_SMAX1	PF23569	186 - 288	2.2e-09	SMAX1 nucleotide binding domain
AAA	PF00004	205 - 318	3.8e-13	ATPase family associated with various cellular activities (AAA)
AAA_lid_9	PF17871	344 - 444	2.2e-34	AAA lid domain
AAA_2	PF07724	598 - 762	9.3e-69	AAA domain (Cdc48 subfamily)
AAA_22	PF13401	600 - 697	1.7e-06	AAA domain
Sigma54_activat	PF00158	601 - 718	9.1e-06	Sigma-54 interaction domain
AAA_5	PF07728	602 - 721	3.2e-12	AAA domain (dynein-related subfamily)
AAA	PF00004	603 - 738	1e-10	ATPase family associated with various cellular activities (AAA)
ClpB_D2-small	PF10431	768 - 847	1.9e-23	C-terminal, D2-small domain, of ClpB protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002819)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74310 AT1G74310 AT4G14670
fragaria_vesca	FvH4_5g14020 FvH4_5g14020 FvH4_5g14020 FvH4_5g14020 FvH4_7g22310 FvH4_7g22310 FvH4_7g22310
malus_domestica	MD06G1201600.v1.1 MD14G1210600.v1.1 MD14G1211000.v1.1
prunus_persica	Prupe.5G203700_v2.0.a1
pyrus_communis	pycom06g17750 pycom14g17400
rosa_chinensis	RchiOBHm_Chr1g0366791 RchiOBHm_Chr7g0182871 RchiOBHm_Chr7g0182901
rosa_laevigata	RLG00000005096 RLG00000005098 RLG00000027319
rosa_multiflora	Rmu_sc0000014.1_g000026 Rmu_sc0000318.1_g000026
rosa_roxburghii	Rroxscaffold_3G00230950 Rroxscaffold_3G00270960
rosa_rugosa	Rorug01G0331900 Rorug01G0333200 Rorug06G0451000
rosa_samantha	Rh1AG340000 Rh7AG054200 Rh7AG403300 Rh7BG053700 Rh7CG055300 Rh7DG053500 Rh7DG053600
rosa_wichuraiana	Rw0G008600 Rw1G030200 Rw7G004420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	34, 2380
Acc16I	TGCGCA	1 cut(s)	1017
Acc36I	ACCTGC	1 cut(s)	889
AccB1I	GGYRCC	1 cut(s)	1044
AccB7I	CCANNNNNTGG	1 cut(s)	1621
AccBSI	CCGCTC	2 cut(s)	50, 94
AccI	GTMKAC	1 cut(s)	1528
AccII	CGCG	1 cut(s)	481
AccIII	TCCGGA	1 cut(s)	925
AclWI	GGATC	5 cut(s)	422, 709, 1696, 2180, 2315
AcoI	YGGCCR	4 cut(s)	42, 73, 1546, 2506
AcsI	RAATTY	1 cut(s)	987
AcuI	CTGAAG	4 cut(s)	233, 692, 723, 783
AcvI	CACGTG	2 cut(s)	79, 2198
AcyI	GRCGYC	2 cut(s)	69, 1045
AfaI	GTAC	5 cut(s)	252, 262, 647, 2008, 2429
AfiI	CCNNNNNNNGG	9 cut(s)	437, 524, 1060, 1384, 1621, 2056, 2330, 2450, 2515
AflII	CTTAAG	1 cut(s)	1319
AjiI	CACGTC	1 cut(s)	1826
AjnI	CCWGG	7 cut(s)	204, 517, 1382, 1548, 1614, 1843, 2487
Alw21I	GWGCWC	3 cut(s)	628, 1341, 2338
Alw26I	GTCTC	3 cut(s)	42, 180, 1245
AlwI	GGATC	5 cut(s)	422, 709, 1696, 2180, 2315
AlwNI	CAGNNNCTG	2 cut(s)	211, 1361
Ama87I	CYCGRG	1 cut(s)	309
Aor13HI	TCCGGA	1 cut(s)	925
ApeKI	GCWGC	8 cut(s)	165, 779, 875, 1013, 1156, 1388, 1675, 2243
ApoI	RAATTY	1 cut(s)	987
Asp700I	GAANNNNTTC	1 cut(s)	2089
AspLEI	GCGC	7 cut(s)	211, 292, 301, 1018, 1047, 1180, 1838
AspS9I	GGNCC	4 cut(s)	1487, 1706, 1741, 2441
AsuC2I	CCSGG	1 cut(s)	2468
AsuHPI	GGTGA	9 cut(s)	289, 424, 478, 724, 1592, 1931, 2140, 2383, 2494
AsuII	TTCGAA	2 cut(s)	985, 1312
AsuNHI	GCTAGC	1 cut(s)	1202
AvaI	CYCGRG	1 cut(s)	309
AvaII	GGWCC	2 cut(s)	1487, 1706
BalI	TGGCCA	1 cut(s)	1548
BanI	GGYRCC	1 cut(s)	1044
BanII	GRGCYC	1 cut(s)	1341
BauI	CACGAG	2 cut(s)	629, 2106
BbrPI	CACGTG	2 cut(s)	79, 2198
BbsI	GAAGAC	1 cut(s)	536
Bbv12I	GWGCWC	3 cut(s)	628, 1341, 2338
BbvI	GCAGC	8 cut(s)	177, 766, 862, 1000, 1168, 1375, 1687, 2230
BccI	CCATC	5 cut(s)	105, 1056, 1973, 1988, 2346
BceAI	ACGGC	2 cut(s)	1500, 1625
BcgI	CGANNNNNNTGC	2 cut(s)	2087, 2121
BciT130I	CCWGG	7 cut(s)	206, 519, 1384, 1550, 1616, 1845, 2489
BciVI	GTATCC	1 cut(s)	922
BclI	TGATCA	2 cut(s)	334, 2031
BcnI	CCSGG	1 cut(s)	2468
BcoDI	GTCTC	3 cut(s)	42, 180, 1245
BfaI	CTAG	7 cut(s)	582, 807, 842, 1203, 1379, 1973, 2220
BfmI	CTRYAG	1 cut(s)	1944
BfoI	RGCGCY	3 cut(s)	212, 1048, 1839
BfrI	CTTAAG	1 cut(s)	1319
BfuAI	ACCTGC	1 cut(s)	889
BfuI	GTATCC	1 cut(s)	922
BlpI	GCTNAGC	2 cut(s)	1437, 1749
BmcAI	AGTACT	1 cut(s)	262
Bme1390I	CCNGG	8 cut(s)	206, 519, 1384, 1550, 1616, 1845, 2468, 2489
Bme18I	GGWCC	2 cut(s)	1487, 1706
BmeT110I	CYCGRG	1 cut(s)	309
BmgBI	CACGTC	1 cut(s)	1826
BmgT120I	GGNCC	4 cut(s)	1487, 1706, 1741, 2441
BmiI	GGNNCC	3 cut(s)	915, 1046, 1707
BmrFI	CCNGG	8 cut(s)	206, 519, 1384, 1550, 1616, 1845, 2468, 2489
BmrI	ACTGGG	2 cut(s)	912, 2090
BmsI	GCATC	7 cut(s)	766, 789, 862, 948, 982, 1615, 2551
BmtI	GCTAGC	1 cut(s)	1206
BmuI	ACTGGG	2 cut(s)	912, 2090
BpiI	GAAGAC	1 cut(s)	536
BplI	GAGNNNNNCTC	6 cut(s)	250, 282, 801, 833, 1242, 1274
BpmI	CTGGAG	1 cut(s)	540
Bpu1102I	GCTNAGC	2 cut(s)	1437, 1749
Bpu14I	TTCGAA	2 cut(s)	985, 1312
BpuEI	CTTGAG	2 cut(s)	1267, 2378
BpuMI	CCSGG	1 cut(s)	2468
BsaAI	YACGTR	3 cut(s)	79, 907, 2198
BsaBI	GATNNNNATC	1 cut(s)	2565
BsaHI	GRCGYC	2 cut(s)	69, 1045
BsaJI	CCNNGG	4 cut(s)	310, 1002, 1383, 2050
BsaWI	WCCGGW	4 cut(s)	499, 819, 925, 2323
BsaXI	ACNNNNNCTCC	2 cut(s)	2475, 2505
Bsc4I	CCNNNNNNNGG	9 cut(s)	437, 524, 1060, 1384, 1621, 2056, 2330, 2450, 2515
Bse118I	RCCGGY	5 cut(s)	71, 128, 158, 253, 2504
Bse1I	ACTGG	6 cut(s)	918, 1528, 1532, 1690, 2077, 2085
Bse3DI	GCAATG	2 cut(s)	396, 1497
Bse8I	GATNNNNATC	1 cut(s)	2565
BseAI	TCCGGA	1 cut(s)	925
BseBI	CCWGG	7 cut(s)	206, 519, 1384, 1550, 1616, 1845, 2489
BseDI	CCNNGG	4 cut(s)	310, 1002, 1383, 2050
BseJI	GATNNNNATC	1 cut(s)	2565
BseLI	CCNNNNNNNGG	9 cut(s)	437, 524, 1060, 1384, 1621, 2056, 2330, 2450, 2515
BseMI	GCAATG	2 cut(s)	396, 1497
BseMII	CTCAG	7 cut(s)	569, 1451, 1719, 1740, 1788, 2198, 2247
BseNI	ACTGG	6 cut(s)	918, 1528, 1532, 1690, 2077, 2085
BseRI	GAGGAG	8 cut(s)	185, 464, 677, 710, 860, 1349, 1472, 1830
BseXI	GCAGC	8 cut(s)	177, 766, 862, 1000, 1168, 1375, 1687, 2230
BsgI	GTGCAG	1 cut(s)	101
Bsh1236I	CGCG	1 cut(s)	481
Bsh1285I	CGRYCG	1 cut(s)	1673
BshNI	GGYRCC	1 cut(s)	1044
BsiEI	CGRYCG	1 cut(s)	1673
BsiHKAI	GWGCWC	3 cut(s)	628, 1341, 2338
BsiHKCI	CYCGRG	1 cut(s)	309
BslFI	GGGAC	2 cut(s)	292, 329
BslI	CCNNNNNNNGG	9 cut(s)	437, 524, 1060, 1384, 1621, 2056, 2330, 2450, 2515
BsmAI	GTCTC	3 cut(s)	42, 180, 1245
BsmFI	GGGAC	2 cut(s)	292, 329
BsmI	GAATGC	2 cut(s)	1158, 2394
BsoBI	CYCGRG	1 cut(s)	309
Bsp119I	TTCGAA	2 cut(s)	985, 1312
Bsp1286I	GDGCHC	3 cut(s)	628, 1341, 2338
Bsp13I	TCCGGA	1 cut(s)	925
Bsp1407I	TGTACA	1 cut(s)	2427
Bsp1720I	GCTNAGC	2 cut(s)	1437, 1749
BspCNI	CTCAG	7 cut(s)	568, 1450, 1720, 1741, 1789, 2199, 2248
BspEI	TCCGGA	1 cut(s)	925
BspFNI	CGCG	1 cut(s)	481
BspHI	TCATGA	2 cut(s)	52, 1858
BspLI	GGNNCC	3 cut(s)	915, 1046, 1707
BspMI	ACCTGC	1 cut(s)	889
BspOI	GCTAGC	1 cut(s)	1206
BspPI	GGATC	5 cut(s)	422, 709, 1696, 2180, 2315
BspT104I	TTCGAA	2 cut(s)	985, 1312
BspT107I	GGYRCC	1 cut(s)	1044
BspTI	CTTAAG	1 cut(s)	1319
BsrBI	CCGCTC	2 cut(s)	50, 94
BsrDI	GCAATG	2 cut(s)	396, 1497
BsrFI	RCCGGY	5 cut(s)	71, 128, 158, 253, 2504
BsrGI	TGTACA	1 cut(s)	2427
BsrI	ACTGG	6 cut(s)	918, 1528, 1532, 1690, 2077, 2085
BssAI	RCCGGY	5 cut(s)	71, 128, 158, 253, 2504
BssECI	CCNNGG	4 cut(s)	310, 1002, 1383, 2050
BssNAI	GTATAC	1 cut(s)	1529
BssNI	GRCGYC	2 cut(s)	69, 1045
BssSI	CACGAG	2 cut(s)	629, 2106
BssT1I	CCWWGG	2 cut(s)	1002, 2050
Bst1107I	GTATAC	1 cut(s)	1529
Bst2BI	CACGAG	2 cut(s)	629, 2106
Bst2UI	CCWGG	7 cut(s)	206, 519, 1384, 1550, 1616, 1845, 2489
Bst4CI	ACNGT	8 cut(s)	934, 1023, 1115, 1483, 1948, 2011, 2029, 2425
Bst6I	CTCTTC	3 cut(s)	1119, 2357, 2397
BstACI	GRCGYC	2 cut(s)	69, 1045
BstAFI	CTTAAG	1 cut(s)	1319
BstAPI	GCANNNNNTGC	1 cut(s)	1625
BstAUI	TGTACA	1 cut(s)	2427
BstBAI	YACGTR	3 cut(s)	79, 907, 2198
BstBI	TTCGAA	2 cut(s)	985, 1312
BstC8I	GCNNGC	8 cut(s)	64, 73, 130, 160, 394, 804, 1204, 1734
BstDEI	CTNAG	7 cut(s)	555, 1437, 1728, 1749, 1797, 2207, 2256
BstENI	CCTNNNNNAGG	2 cut(s)	435, 2448
BstFNI	CGCG	1 cut(s)	481
BstH2I	RGCGCY	3 cut(s)	212, 1048, 1839
BstHHI	GCGC	7 cut(s)	211, 292, 301, 1018, 1047, 1180, 1838
BstMAI	GTCTC	3 cut(s)	42, 180, 1245
BstMCI	CGRYCG	1 cut(s)	1673
BstNI	CCWGG	7 cut(s)	206, 519, 1384, 1550, 1616, 1845, 2489
BstSCI	CCNGG	8 cut(s)	204, 517, 1382, 1548, 1614, 1843, 2466, 2487
BstSFI	CTRYAG	1 cut(s)	1944
BstUI	CGCG	1 cut(s)	481
BstV1I	GCAGC	8 cut(s)	177, 766, 862, 1000, 1168, 1375, 1687, 2230
BstV2I	GAAGAC	1 cut(s)	536
BstZ17I	GTATAC	1 cut(s)	1529
BsuI	GTATCC	1 cut(s)	922
BtrI	CACGTC	1 cut(s)	1826
BtsI	GCAGTG	1 cut(s)	1626
BtsIMutI	CAGTG	3 cut(s)	925, 1626, 2034
BveI	ACCTGC	1 cut(s)	889
Cac8I	GCNNGC	8 cut(s)	64, 73, 130, 160, 394, 804, 1204, 1734
CaiI	CAGNNNCTG	2 cut(s)	211, 1361
CciI	TCATGA	2 cut(s)	52, 1858
CfoI	GCGC	7 cut(s)	211, 292, 301, 1018, 1047, 1180, 1838
Cfr10I	RCCGGY	5 cut(s)	71, 128, 158, 253, 2504
Cfr13I	GGNCC	4 cut(s)	1487, 1706, 1741, 2441
CseI	GACGC	3 cut(s)	77, 2143, 2444
Csp6I	GTAC	5 cut(s)	251, 261, 646, 2007, 2428
CviAII	CATG	6 cut(s)	53, 619, 977, 1175, 1859, 2617
CviQI	GTAC	5 cut(s)	251, 261, 646, 2007, 2428
DdeI	CTNAG	7 cut(s)	555, 1437, 1728, 1749, 1797, 2207, 2256
DinI	GGCGCC	1 cut(s)	1046
DrdI	GACNNNNNNGTC	2 cut(s)	34, 2380
DseDI	GACNNNNNNGTC	2 cut(s)	34, 2380
EaeI	YGGCCR	4 cut(s)	42, 73, 1546, 2506
Eam1104I	CTCTTC	3 cut(s)	1119, 2357, 2397
EarI	CTCTTC	3 cut(s)	1119, 2357, 2397
EciI	GGCGGA	4 cut(s)	228, 926, 1909, 2072
Ecl136II	GAGCTC	1 cut(s)	1339
Eco130I	CCWWGG	2 cut(s)	1002, 2050
Eco147I	AGGCCT	1 cut(s)	2487
Eco24I	GRGCYC	1 cut(s)	1341
Eco47I	GGWCC	2 cut(s)	1487, 1706
Eco53kI	GAGCTC	1 cut(s)	1339
Eco57I	CTGAAG	4 cut(s)	233, 692, 723, 783
Eco72I	CACGTG	2 cut(s)	79, 2198
Eco88I	CYCGRG	1 cut(s)	309
EcoICRI	GAGCTC	1 cut(s)	1339
EcoNI	CCTNNNNNAGG	2 cut(s)	435, 2448
EcoO109I	RGGNCCY	2 cut(s)	1741, 2441
EcoRII	CCWGG	7 cut(s)	204, 517, 1382, 1548, 1614, 1843, 2487
EcoT14I	CCWWGG	2 cut(s)	1002, 2050
EcoT38I	GRGCYC	1 cut(s)	1341
EgeI	GGCGCC	1 cut(s)	1046
EheI	GGCGCC	1 cut(s)	1046
ErhI	CCWWGG	2 cut(s)	1002, 2050
FaeI	CATG	6 cut(s)	56, 622, 980, 1178, 1862, 2620
FalI	AAGNNNNNCTT	4 cut(s)	1155, 1187, 1200, 1232
FaqI	GGGAC	2 cut(s)	292, 329
FatI	CATG	6 cut(s)	52, 618, 976, 1174, 1858, 2616
FauI	CCCGC	2 cut(s)	133, 628
FbaI	TGATCA	2 cut(s)	334, 2031
FblI	GTMKAC	1 cut(s)	1528
FriOI	GRGCYC	1 cut(s)	1341
FspBI	CTAG	7 cut(s)	582, 807, 842, 1203, 1379, 1973, 2220
FspI	TGCGCA	1 cut(s)	1017
GlaI	GCGC	7 cut(s)	210, 291, 300, 1017, 1046, 1179, 1837
GsuI	CTGGAG	1 cut(s)	540
HaeII	RGCGCY	3 cut(s)	212, 1048, 1839
HgaI	GACGC	3 cut(s)	77, 2143, 2444
HhaI	GCGC	7 cut(s)	211, 292, 301, 1018, 1047, 1180, 1838
Hin1I	GRCGYC	2 cut(s)	69, 1045
Hin1II	CATG	6 cut(s)	56, 622, 980, 1178, 1862, 2620
Hin6I	GCGC	7 cut(s)	209, 290, 299, 1016, 1045, 1178, 1836
HinP1I	GCGC	7 cut(s)	209, 290, 299, 1016, 1045, 1178, 1836
HincII	GTYRAC	2 cut(s)	1118, 1473
HindII	GTYRAC	2 cut(s)	1118, 1473
HindIII	AAGCTT	1 cut(s)	218
HphI	GGTGA	9 cut(s)	289, 424, 478, 724, 1592, 1931, 2140, 2383, 2494
Hpy99I	CGWCG	2 cut(s)	71, 229
HpyCH4III	ACNGT	8 cut(s)	934, 1023, 1115, 1483, 1948, 2011, 2029, 2425
HpyCH4IV	ACGT	4 cut(s)	78, 906, 1825, 2197
HpyF3I	CTNAG	7 cut(s)	555, 1437, 1728, 1749, 1797, 2207, 2256
HpySE526I	ACGT	4 cut(s)	78, 906, 1825, 2197
Hsp92I	GRCGYC	2 cut(s)	69, 1045
Hsp92II	CATG	6 cut(s)	56, 622, 980, 1178, 1862, 2620
HspAI	GCGC	7 cut(s)	209, 290, 299, 1016, 1045, 1178, 1836
KasI	GGCGCC	1 cut(s)	1044
Kpn2I	TCCGGA	1 cut(s)	925
KroI	GCCGGC	3 cut(s)	71, 128, 158
KroNI	GCCGGC	3 cut(s)	73, 130, 160
Ksp22I	TGATCA	2 cut(s)	334, 2031
LmnI	GCTCC	8 cut(s)	99, 244, 421, 822, 1263, 1336, 1807, 1843
Lsp1109I	GCAGC	8 cut(s)	177, 766, 862, 1000, 1168, 1375, 1687, 2230
LweI	GCATC	7 cut(s)	766, 789, 862, 948, 982, 1615, 2551
MaeI	CTAG	7 cut(s)	582, 807, 842, 1203, 1379, 1973, 2220
MaeII	ACGT	4 cut(s)	78, 906, 1825, 2197
MaeIII	GTNAC	6 cut(s)	584, 1400, 1534, 2278, 2371, 2500
MbiI	CCGCTC	2 cut(s)	50, 94
MfeI	CAATTG	1 cut(s)	1065
MhlI	GDGCHC	3 cut(s)	628, 1341, 2338
MlsI	TGGCCA	1 cut(s)	1548
MluCI	AATT	5 cut(s)	987, 1065, 1569, 1776, 1785
MluNI	TGGCCA	1 cut(s)	1548
Mly113I	GGCGCC	1 cut(s)	1045
MlyI	GAGTC	6 cut(s)	44, 182, 356, 832, 1036, 1110
MmeI	TCCRAC	5 cut(s)	144, 1285, 1465, 1499, 1733
Mox20I	TGGCCA	1 cut(s)	1548
MroI	TCCGGA	1 cut(s)	925
MroNI	GCCGGC	3 cut(s)	71, 128, 158
MroXI	GAANNNNTTC	1 cut(s)	2089
MscI	TGGCCA	1 cut(s)	1548
MseI	TTAA	7 cut(s)	273, 1320, 1352, 1568, 1988, 2493, 2577
MslI	CAYNNNNRTG	2 cut(s)	978, 2621
Msp20I	TGGCCA	1 cut(s)	1548
MspA1I	CMGCKG	1 cut(s)	1159
MspCI	CTTAAG	1 cut(s)	1319
MspR9I	CCNGG	8 cut(s)	206, 519, 1384, 1550, 1616, 1845, 2468, 2489
MunI	CAATTG	1 cut(s)	1065
Mva1269I	GAATGC	2 cut(s)	1158, 2394
MvaI	CCWGG	7 cut(s)	206, 519, 1384, 1550, 1616, 1845, 2489
MvnI	CGCG	1 cut(s)	481
NaeI	GCCGGC	3 cut(s)	73, 130, 160
NarI	GGCGCC	1 cut(s)	1045
NciI	CCSGG	1 cut(s)	2468
NgoMIV	GCCGGC	3 cut(s)	71, 128, 158
NheI	GCTAGC	1 cut(s)	1202
NlaIII	CATG	6 cut(s)	56, 622, 980, 1178, 1862, 2620
NlaIV	GGNNCC	3 cut(s)	915, 1046, 1707
NmeAIII	GCCGAG	1 cut(s)	1192
NmuCI	GTSAC	3 cut(s)	2278, 2371, 2500
NsbI	TGCGCA	1 cut(s)	1017
NspV	TTCGAA	2 cut(s)	985, 1312
PagI	TCATGA	2 cut(s)	52, 1858
PceI	AGGCCT	1 cut(s)	2487
PcsI	WCGNNNNNNNCGW	2 cut(s)	75, 485
PctI	GAATGC	2 cut(s)	1158, 2394
PdiI	GCCGGC	3 cut(s)	73, 130, 160
PdmI	GAANNNNTTC	1 cut(s)	2089
PfeI	GAWTC	8 cut(s)	4, 280, 343, 472, 1309, 1424, 2158, 2527
PflMI	CCANNNNNTGG	1 cut(s)	1621
PfoI	TCCNGGA	1 cut(s)	1843
PleI	GAGTC	6 cut(s)	43, 181, 356, 832, 1035, 1109
PluTI	GGCGCC	1 cut(s)	1048
PmaCI	CACGTG	2 cut(s)	79, 2198
PmlI	CACGTG	2 cut(s)	79, 2198
PpsI	GAGTC	6 cut(s)	43, 181, 356, 832, 1035, 1109
Ppu21I	YACGTR	3 cut(s)	79, 907, 2198
Psp124BI	GAGCTC	1 cut(s)	1341
Psp6I	CCWGG	7 cut(s)	204, 517, 1382, 1548, 1614, 1843, 2487
PspCI	CACGTG	2 cut(s)	79, 2198
PspGI	CCWGG	7 cut(s)	204, 517, 1382, 1548, 1614, 1843, 2487
PspN4I	GGNNCC	3 cut(s)	915, 1046, 1707
PspPI	GGNCC	4 cut(s)	1487, 1706, 1741, 2441
PstNI	CAGNNNCTG	2 cut(s)	211, 1361
PvuII	CAGCTG	1 cut(s)	1159
RsaI	GTAC	5 cut(s)	252, 262, 647, 2008, 2429
RsaNI	GTAC	5 cut(s)	251, 261, 646, 2007, 2428
RseI	CAYNNNNRTG	2 cut(s)	978, 2621
SacI	GAGCTC	1 cut(s)	1341
SaqAI	TTAA	7 cut(s)	273, 1320, 1352, 1568, 1988, 2493, 2577
Sau96I	GGNCC	4 cut(s)	1487, 1706, 1741, 2441
ScaI	AGTACT	1 cut(s)	262
SchI	GAGTC	6 cut(s)	44, 182, 356, 832, 1036, 1110
ScrFI	CCNGG	8 cut(s)	206, 519, 1384, 1550, 1616, 1845, 2468, 2489
SduI	GDGCHC	3 cut(s)	628, 1341, 2338
SfaNI	GCATC	7 cut(s)	766, 789, 862, 948, 982, 1615, 2551
SfcI	CTRYAG	1 cut(s)	1944
SfoI	GGCGCC	1 cut(s)	1046
SfuI	TTCGAA	2 cut(s)	985, 1312
SinI	GGWCC	2 cut(s)	1487, 1706
SmiMI	CAYNNNNRTG	2 cut(s)	978, 2621
SmlI	CTYRAG	3 cut(s)	1246, 1319, 2357
SmoI	CTYRAG	3 cut(s)	1246, 1319, 2357
Sse9I	AATT	5 cut(s)	987, 1065, 1569, 1776, 1785
SseBI	AGGCCT	1 cut(s)	2487
SspDI	GGCGCC	1 cut(s)	1044
SspMI	CTAG	7 cut(s)	582, 807, 842, 1203, 1379, 1973, 2220
SstI	GAGCTC	1 cut(s)	1341
StuI	AGGCCT	1 cut(s)	2487
StyD4I	CCNGG	8 cut(s)	204, 517, 1382, 1548, 1614, 1843, 2466, 2487
StyI	CCWWGG	2 cut(s)	1002, 2050
TaaI	ACNGT	8 cut(s)	934, 1023, 1115, 1483, 1948, 2011, 2029, 2425
TaiI	ACGT	4 cut(s)	81, 909, 1828, 2200
TaqI	TCGA	8 cut(s)	985, 1025, 1030, 1312, 1761, 2113, 2184, 2338
TasI	AATT	5 cut(s)	987, 1065, 1569, 1776, 1785
TatI	WGTACW	3 cut(s)	260, 645, 2427
TauI	GCSGC	6 cut(s)	47, 50, 94, 165, 404, 407
TfiI	GAWTC	8 cut(s)	4, 280, 343, 472, 1309, 1424, 2158, 2527
Tru1I	TTAA	7 cut(s)	273, 1320, 1352, 1568, 1988, 2493, 2577
Tru9I	TTAA	7 cut(s)	273, 1320, 1352, 1568, 1988, 2493, 2577
TscAI	CASTG	3 cut(s)	925, 1626, 2034
TseFI	GTSAC	3 cut(s)	2278, 2371, 2500
TseI	GCWGC	8 cut(s)	165, 779, 875, 1013, 1156, 1388, 1675, 2243
Tsp45I	GTSAC	3 cut(s)	2278, 2371, 2500
TspGWI	ACGGA	3 cut(s)	128, 213, 1895
TspRI	CASTG	3 cut(s)	925, 1626, 2034
Van91I	CCANNNNNTGG	1 cut(s)	1621
Vha464I	CTTAAG	1 cut(s)	1319
VpaK11BI	GGWCC	2 cut(s)	1487, 1706
XagI	CCTNNNNNAGG	2 cut(s)	435, 2448
XapI	RAATTY	1 cut(s)	987
XbaI	TCTAGA	2 cut(s)	841, 1972
XcmI	CCANNNNNNNNNTGG	2 cut(s)	37, 743
XmiI	GTMKAC	1 cut(s)	1528
XmnI	GAANNNNTTC	1 cut(s)	2089
XspI	CTAG	7 cut(s)	582, 807, 842, 1203, 1379, 1973, 2220
ZrmI	AGTACT	1 cut(s)	262

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.