Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0002819

Physical Location & Seq

Genomic Coordinates Reverse (-)

64991118 .. 64994609

Gene Structure 3492 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000005096

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2712 bp

ATGAATCCTGACAAATTTACTCACAAGACAAATGAGTCTCTGGCTGCGGCTCATGAACTGGCATCGGATGCTGGGCATGTGCAGTTCACGCCTCTCCATCTTGCTACCGCATTGGTTTCGGACCCTGCCGGCATTTTTCGACAGGCGATTGCCAATGCTAGTGAGAGAGTTTTCAATCAGGCATTGAAGAAGCTCCCGTCTCAGTCCCCTCCCCCGGATCAAGTACTGGCCAGCACTACTCTCATTAAGGCAATTCAGAGAGCGCAGTCCGCGCAGAAAGATCGAGGGGATACACATTTGGCTGTTGATCAGTTGATTCTTGGCCTACTGGAGGATTCGCAGATTGGGGACTTGCTGAAAGAAGCTGGCATTGCTACAGCGAAGGTGAAGTCGGAAGTTCAAAAGCTTTGTGGTAAAGAGGGGAAGAAGGTGGAGAGTGCCTCTGGGGACACGAATTTCCAAGCTTTGAATACTTATGGGAGAGACCTTGTTGAGCAGGCCGGGAAGCTTGACCCAGTGATTGGTAGAGATGAGGAGATAAGGTGGGTGGTTCAGATTCTCTCAAGGAGAACGAAGAATAACCCAGTGCTAATTGGAGAGCCAGGAGTTGGGAAAACGGCTGTAGTTGAAGGTTTGGCTCAGAGGATAGTAAGAGGGGATGTCCCTAGTAATCTGGCAGATGTGAGGCTTATCGCATTGGACATGGGTGCTCTAGTAGCTGGTGCCAAGTATAGAGGAGAATTTGAGGAGAGGTTGAAGGCTGTCTTGAAAGAAGTAGAAGAGGCCGAAGGGAAGGTGATTCTGTTTATCGATGAAATCCACCTTGTTCTTGGTGCTGGTAGAACAGAGGGGTCTATGGATGCCGCCAACCTTTTCAAGCCAATGCTCGCTAGAGGTCAACTCCGGTGCATCGGGGCAACGACTCTTGAAGAGTACAGGAAATATGTGGAGAAGGATGCTGCATTTGAGCGAAGGTTTCAACAAGTTTATGTGGCGGAACCTAGTGTTCCAGTCACTTTTAGCATCCTTCGAGGCTTGAAGGAGAGATATGAAGGGCATCATGGTGTTAGAATTTTGGATAGAGCTTTAGTTGAAGCTGCACAACTATCGAGTCGATACATCACAGGGCGCCATCTCCCTGACAAGGCAATTGATCTAGTTGATGAAGCTTGTGCACATGTGAGAGTCCAGCTTGACAGTCAACCAGAAGAAATTGACAACCTTGAGAGAAAGAGGATGCAGCTGGAAGTTGAGCTTCATGCGCTTGAAAAAGAAAAGGATAAAGCTAGCAAAGCTCGTCTTGTTGAGGTTCGGAGGGAGCTTGATGACTTAAGAGACAAGCTTCAGCCCTTGATGATGAAGTATAGAAAAGAGAAGGAAAGGATTGATGAGATTCGAAGGCTTAAGAAAAAGCGTGAAGAGCTCTTATTTGCTTTGACAGAGGCTGAAAGAAGATATGATTTAGCCAGGGCAGCTGATTTGCGTTATGGGGATATTCAGGAAGTGGAGTCTGCTATTGACCAGCTTGAAGGAAGCACAGAAGAGAAGTTGATGTTGACTGAGACTGTTGGACCAGAACATATTGCAGAGGTTGTAAGCCGTTGGACCGGTATACCAGTAACAAGGCTCTGCCAGGATGACAAAGAGAGGTTAATTGGACTTGGTGAGAGATTGCATAAGAGAGTAGTGGGTCAAAACCAGGCAGTTGATGCTGTTGCAGAAGCCGTATTGAGATCAAGAGCTGGGTTAGGCAGACCACAACAACCAACTGGATCATTCCTTTTCTTGGGTCCAACTGGTGTTGGTAAAACTGAGCTTGCAAAGGCTCTTGCTGAACAACTTTTTGATGACGAAAATCTAATTGTTCGAATTGATATGTCTGAGTATATGGAGCAACATTCTGTTGCACGTCTGATTGGTGCTCCTCCTGGATATGTTGGTCATGAAGAAGGTGGACAACTCACAGAGGCAGTGAGGCGGAGGCCTTATAGTGTTATACTGTTTGATGAAGTGGAGAAAGCACACCCTGCAGTTTTCAATACTCTCCTGCAAGTTTTAGATGATGGGAGGTTAACTGATGTTCAAGGTCGTACAGTTAATTTTAGGAATACAGTGATCATTATGACTTCAAACCTTGGGGCGGAACATCTCCGAACTGGTTTGATGGGCAAGTGTTCAATGCAGGTTGCTTTTGATCAAGTTATGCAAGAGGTGAGAACATACTTTAGGCCTGAATTGCTAAATCGGCTTGATGAGGTAGTTGTTTTCGATCCTCTTTCACGTGAGCAACTGGGGAAGGTGGCTAGGTTACAAATGAAGGACGTTGCTGTCCGGCTTGCTGAGAGGGATGTTGCTTTGGTGGTCACCGATAAAGCATTCGATTATATTCTGGACGAGAGTGATGATCCGGTTTATGGTGCTCGACCTATAAGAAGATGGCTTGAGAAGAGAGTGGTGACAGAGCTATCAAGAATGCTGATTAGAAAAGAGATAGATGAGAACTCAACTGTGTACATTGATGTAGGACCCAAGGCAAATGAGTTGGTTTACCGGGTTGAGAAGAATGGAGGGCTTGTTAATGCTGTCACCGGTCAGAAGTCAGATGTGTTGATACAACTTCCGAATGGACCAAGGCCGATTGATGATGCTCATCAGGCAGTTAAGAAGATGAAGATCGAGGAAATGGATGAGGACCTCGACATGGATGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006417 GO:0006950 GO:0006979 GO:0008150 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009636 GO:0009642 GO:0009644 GO:0009889 GO:0009891 GO:0009893 GO:0009941 GO:0009987 GO:0010035 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031967 GO:0031975 GO:0032268 GO:0032270 GO:0033554 GO:0034248 GO:0034250 GO:0034605 GO:0042221 GO:0042493 GO:0042542 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043335 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045727 GO:0046677 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051716 GO:0060255 GO:0065007 GO:0080090 GO:1901700 GO:2000112

KEGG Pathways

Metabolic & Signaling

ko04213 map04213

Pfam Domains

Protein Families

AAA AAA_2 ClpB_D2-small Clp_N

Protein Analysis

904

Amino Acids

101.08

Weight (kDa)

5.65

Isoelectric Point (pI)

35.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Clp_N	PF02861	5 - 121	1.7e-23	Clp repeat (R) N-terminal domain
AAA_16	PF13191	173 - 224	8.1e-06	AAA ATPase domain
NBD_SMAX1	PF23569	177 - 279	9.1e-10	SMAX1 nucleotide binding domain
AAA	PF00004	196 - 309	3.8e-13	ATPase family associated with various cellular activities (AAA)
AAA_lid_9	PF17871	337 - 435	4.8e-34	AAA lid domain
AAA_2	PF07724	589 - 753	2.4e-67	AAA domain (Cdc48 subfamily)
AAA_22	PF13401	591 - 688	1.6e-06	AAA domain
Sigma54_activat	PF00158	592 - 711	1.1e-06	Sigma-54 interaction domain
AAA_5	PF07728	593 - 712	2.8e-12	AAA domain (dynein-related subfamily)
AAA	PF00004	594 - 732	2.2e-11	ATPase family associated with various cellular activities (AAA)
ClpB_D2-small	PF10431	759 - 838	1.7e-20	C-terminal, D2-small domain, of ClpB protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002819)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74310 AT1G74310 AT4G14670
fragaria_vesca	FvH4_5g14020 FvH4_5g14020 FvH4_5g14020 FvH4_5g14020 FvH4_7g22310 FvH4_7g22310 FvH4_7g22310
malus_domestica	MD06G1201600.v1.1 MD14G1210600.v1.1 MD14G1211000.v1.1
prunus_persica	Prupe.5G203700_v2.0.a1
pyrus_communis	pycom06g17750 pycom14g17400
rosa_chinensis	RchiOBHm_Chr1g0366791 RchiOBHm_Chr7g0182871 RchiOBHm_Chr7g0182901
rosa_laevigata	RLG00000005096 RLG00000005098 RLG00000027319
rosa_multiflora	Rmu_sc0000014.1_g000026 Rmu_sc0000318.1_g000026
rosa_roxburghii	Rroxscaffold_3G00230950 Rroxscaffold_3G00270960
rosa_rugosa	Rorug01G0331900 Rorug01G0333200 Rorug06G0451000
rosa_samantha	Rh1AG340000 Rh7AG054200 Rh7AG403300 Rh7BG053700 Rh7CG055300 Rh7DG053500 Rh7DG053600
rosa_wichuraiana	Rw0G008600 Rw1G030200 Rw7G004420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	34, 2327
Acc36I	ACCTGC	1 cut(s)	2173
AccB1I	GGYRCC	2 cut(s)	722, 1128
AccB7I	CCANNNNNTGG	2 cut(s)	521, 608
AccI	GTMKAC	1 cut(s)	1612
AccII	CGCG	1 cut(s)	272
AciI	CCGC	7 cut(s)	47, 108, 270, 864, 995, 1978, 2141
AclWI	GGATC	4 cut(s)	225, 1780, 2264, 2399
AcoI	YGGCCR	1 cut(s)	228
AcsI	RAATTY	4 cut(s)	14, 454, 740, 1071
AcuI	CTGAAG	1 cut(s)	1328
AcvI	CACGTG	1 cut(s)	2282
AcyI	GRCGYC	1 cut(s)	1129
AfaI	GTAC	4 cut(s)	225, 935, 2092, 2513
AfiI	CCNNNNNNNGG	9 cut(s)	214, 331, 521, 608, 1144, 1786, 2140, 2414, 2701
AflII	CTTAAG	2 cut(s)	1330, 1403
AflIII	ACRYGT	1 cut(s)	1177
AgeI	ACCGGT	2 cut(s)	1607, 2588
AjiI	CACGTC	1 cut(s)	1910
AjnI	CCWGG	5 cut(s)	601, 1466, 1632, 1698, 1927
Alw21I	GWGCWC	5 cut(s)	712, 1177, 1425, 1924, 2422
Alw26I	GTCTC	5 cut(s)	42, 204, 477, 1329, 1556
Alw44I	GTGCAC	1 cut(s)	1173
AlwI	GGATC	4 cut(s)	225, 1780, 2264, 2399
AlwNI	CAGNNNCTG	1 cut(s)	1445
AoxI	GGCC	7 cut(s)	228, 322, 498, 783, 1982, 2228, 2633
ApaLI	GTGCAC	1 cut(s)	1173
ApeKI	GCWGC	5 cut(s)	44, 959, 1097, 1240, 1472
ApoI	RAATTY	4 cut(s)	14, 454, 740, 1071
ArsI	GACNNNNNNTTYG	2 cut(s)	374, 406
AsiGI	ACCGGT	2 cut(s)	1607, 2588
AspLEI	GCGC	4 cut(s)	265, 274, 1131, 1264
AspS9I	GGNCC	7 cut(s)	121, 1571, 1605, 1790, 2525, 2627, 2692
AsuC2I	CCSGG	3 cut(s)	215, 502, 2552
AsuHPI	GGTGA	7 cut(s)	397, 808, 1676, 2224, 2356, 2467, 2578
AsuII	TTCGAA	2 cut(s)	1396, 1867
AsuNHI	GCTAGC	1 cut(s)	1286
AvaII	GGWCC	7 cut(s)	121, 1571, 1605, 1790, 2525, 2627, 2692
BaeGI	GKGCMC	1 cut(s)	1177
BalI	TGGCCA	1 cut(s)	230
BanI	GGYRCC	2 cut(s)	722, 1128
BanII	GRGCYC	1 cut(s)	1425
BbrPI	CACGTG	1 cut(s)	2282
Bbv12I	GWGCWC	5 cut(s)	712, 1177, 1425, 1924, 2422
BbvI	GCAGC	5 cut(s)	31, 946, 1084, 1252, 1484
BccI	CCATC	5 cut(s)	105, 1140, 2057, 2158, 2430
BceAI	ACGGC	3 cut(s)	633, 1584, 1709
BcgI	CGANNNNNNTGC	6 cut(s)	99, 133, 263, 297, 1089, 1123
BciT130I	CCWGG	5 cut(s)	603, 1468, 1634, 1700, 1929
BciVI	GTATCC	1 cut(s)	283
BclI	TGATCA	3 cut(s)	307, 2115, 2194
BcnI	CCSGG	3 cut(s)	215, 502, 2552
BcoDI	GTCTC	5 cut(s)	42, 204, 477, 1329, 1556
BfaI	CTAG	8 cut(s)	159, 666, 713, 891, 1002, 1157, 1287, 2304
BfmI	CTRYAG	3 cut(s)	375, 621, 2028
BfoI	RGCGCY	1 cut(s)	1132
BfrI	CTTAAG	2 cut(s)	1330, 1403
BfuAI	ACCTGC	1 cut(s)	2173
BfuI	GTATCC	1 cut(s)	283
BisI	GCNGC	7 cut(s)	45, 48, 864, 960, 1098, 1241, 1473
BlsI	GCNGC	7 cut(s)	46, 49, 865, 961, 1099, 1242, 1474
BmcAI	AGTACT	1 cut(s)	225
Bme1390I	CCNGG	8 cut(s)	215, 502, 603, 1468, 1634, 1700, 1929, 2552
Bme18I	GGWCC	7 cut(s)	121, 1571, 1605, 1790, 2525, 2627, 2692
BmgBI	CACGTC	1 cut(s)	1910
BmgT120I	GGNCC	7 cut(s)	121, 1571, 1605, 1790, 2525, 2627, 2692
BmiI	GGNNCC	6 cut(s)	123, 724, 999, 1130, 1791, 2527
BmrFI	CCNGG	8 cut(s)	215, 502, 603, 1468, 1634, 1700, 1929, 2552
BmrI	ACTGGG	3 cut(s)	509, 578, 2300
BmtI	GCTAGC	1 cut(s)	1290
BmuI	ACTGGG	3 cut(s)	509, 578, 2300
BplI	GAGNNNNNCTC	4 cut(s)	425, 457, 885, 917
BpmI	CTGGAG	1 cut(s)	350
Bpu14I	TTCGAA	2 cut(s)	1396, 1867
BpuEI	CTTGAG	3 cut(s)	547, 1244, 2462
BpuMI	CCSGG	3 cut(s)	215, 502, 2552
Bsa29I	ATCGAT	1 cut(s)	810
BsaAI	YACGTR	1 cut(s)	2282
BsaBI	GATNNNNATC	2 cut(s)	2649, 2672
BsaHI	GRCGYC	1 cut(s)	1129
BsaI	GGTCTC	1 cut(s)	477
BsaJI	CCNNGG	5 cut(s)	213, 1467, 2134, 2529, 2630
BsaWI	WCCGGW	4 cut(s)	903, 1607, 2407, 2588
BsaXI	ACNNNNNCTCC	4 cut(s)	527, 557, 2559, 2589
Bsc4I	CCNNNNNNNGG	9 cut(s)	214, 331, 521, 608, 1144, 1786, 2140, 2414, 2701
Bse118I	RCCGGY	3 cut(s)	128, 1607, 2588
Bse3DI	GCAATG	1 cut(s)	369
Bse8I	GATNNNNATC	2 cut(s)	2649, 2672
BseBI	CCWGG	5 cut(s)	603, 1468, 1634, 1700, 1929
BseCI	ATCGAT	1 cut(s)	810
BseDI	CCNNGG	5 cut(s)	213, 1467, 2134, 2529, 2630
BseJI	GATNNNNATC	2 cut(s)	2649, 2672
BseLI	CCNNNNNNNGG	9 cut(s)	214, 331, 521, 608, 1144, 1786, 2140, 2414, 2701
BseMI	GCAATG	1 cut(s)	369
BseMII	CTCAG	6 cut(s)	215, 653, 1551, 1803, 1872, 2331
BseRI	GAGGAG	4 cut(s)	548, 750, 761, 1914
BseSI	GKGCMC	1 cut(s)	1177
BseXI	GCAGC	5 cut(s)	31, 946, 1084, 1252, 1484
BseYI	CCCAGC	2 cut(s)	71, 1742
BsgI	GTGCAG	2 cut(s)	101, 1083
Bsh1236I	CGCG	1 cut(s)	272
BshFI	GGCC	7 cut(s)	230, 324, 500, 785, 1984, 2230, 2635
BshNI	GGYRCC	2 cut(s)	722, 1128
BshTI	ACCGGT	2 cut(s)	1607, 2588
BshVI	ATCGAT	1 cut(s)	810
BsiHKAI	GWGCWC	5 cut(s)	712, 1177, 1425, 1924, 2422
BsiSI	CCGG	9 cut(s)	129, 215, 501, 904, 1608, 2332, 2408, 2551, 2589
BslFI	GGGAC	4 cut(s)	190, 362, 461, 647
BslI	CCNNNNNNNGG	9 cut(s)	214, 331, 521, 608, 1144, 1786, 2140, 2414, 2701
BsmAI	GTCTC	5 cut(s)	42, 204, 477, 1329, 1556
BsmBI	CGTCTC	1 cut(s)	204
BsmFI	GGGAC	4 cut(s)	190, 362, 461, 647
BsmI	GAATGC	2 cut(s)	2375, 2478
BsnI	GGCC	7 cut(s)	230, 324, 500, 785, 1984, 2230, 2635
Bso31I	GGTCTC	1 cut(s)	477
Bsp119I	TTCGAA	2 cut(s)	1396, 1867
Bsp1286I	GDGCHC	5 cut(s)	712, 1177, 1425, 1924, 2422
Bsp1407I	TGTACA	1 cut(s)	2511
BspACI	CCGC	7 cut(s)	47, 108, 270, 864, 995, 1978, 2141
BspANI	GGCC	7 cut(s)	230, 324, 500, 785, 1984, 2230, 2635
BspCNI	CTCAG	6 cut(s)	214, 652, 1552, 1804, 1873, 2332
BspDI	ATCGAT	1 cut(s)	810
BspFNI	CGCG	1 cut(s)	272
BspHI	TCATGA	2 cut(s)	52, 1942
BspLI	GGNNCC	6 cut(s)	123, 724, 999, 1130, 1791, 2527
BspMAI	CTGCAG	1 cut(s)	2032
BspMI	ACCTGC	1 cut(s)	2173
BspOI	GCTAGC	1 cut(s)	1290
BspPI	GGATC	4 cut(s)	225, 1780, 2264, 2399
BspQI	GCTCTTC	1 cut(s)	1413
BspT104I	TTCGAA	2 cut(s)	1396, 1867
BspT107I	GGYRCC	2 cut(s)	722, 1128
BspTI	CTTAAG	2 cut(s)	1330, 1403
BspTNI	GGTCTC	1 cut(s)	477
BsrDI	GCAATG	1 cut(s)	369
BsrFI	RCCGGY	3 cut(s)	128, 1607, 2588
BsrGI	TGTACA	1 cut(s)	2511
BssAI	RCCGGY	3 cut(s)	128, 1607, 2588
BssECI	CCNNGG	5 cut(s)	213, 1467, 2134, 2529, 2630
BssNAI	GTATAC	1 cut(s)	1613
BssNI	GRCGYC	1 cut(s)	1129
BssT1I	CCWWGG	3 cut(s)	2134, 2529, 2630
Bst1107I	GTATAC	1 cut(s)	1613
Bst2UI	CCWGG	5 cut(s)	603, 1468, 1634, 1700, 1929
Bst4CI	ACNGT	6 cut(s)	1199, 1567, 2001, 2095, 2113, 2509
Bst6I	CTCTTC	5 cut(s)	774, 924, 1413, 1536, 2441
BstACI	GRCGYC	1 cut(s)	1129
BstAFI	CTTAAG	2 cut(s)	1330, 1403
BstAPI	GCANNNNNTGC	3 cut(s)	68, 1709, 2027
BstAUI	TGTACA	1 cut(s)	2511
BstBAI	YACGTR	1 cut(s)	2282
BstBI	TTCGAA	2 cut(s)	1396, 1867
BstC8I	GCNNGC	8 cut(s)	130, 232, 367, 498, 888, 1288, 1818, 2337
BstDEI	CTNAG	6 cut(s)	201, 639, 1560, 1812, 1881, 2340
BstEII	GGTNACC	1 cut(s)	2362
BstENI	CCTNNNNNAGG	1 cut(s)	329
BstFNI	CGCG	1 cut(s)	272
BstH2I	RGCGCY	1 cut(s)	1132
BstHHI	GCGC	4 cut(s)	265, 274, 1131, 1264
BstMAI	GTCTC	5 cut(s)	42, 204, 477, 1329, 1556
BstNI	CCWGG	5 cut(s)	603, 1468, 1634, 1700, 1929
BstNSI	RCATGY	2 cut(s)	80, 1181
BstPI	GGTNACC	1 cut(s)	2362
BstSCI	CCNGG	8 cut(s)	213, 500, 601, 1466, 1632, 1698, 1927, 2550
BstSFI	CTRYAG	3 cut(s)	375, 621, 2028
BstSLI	GKGCMC	1 cut(s)	1177
BstUI	CGCG	1 cut(s)	272
BstV1I	GCAGC	5 cut(s)	31, 946, 1084, 1252, 1484
BstZ17I	GTATAC	1 cut(s)	1613
Bsu15I	ATCGAT	1 cut(s)	810
BsuI	GTATCC	1 cut(s)	283
BsuRI	GGCC	7 cut(s)	230, 324, 500, 785, 1984, 2230, 2635
BsuTUI	ATCGAT	1 cut(s)	810
BtrI	CACGTC	1 cut(s)	1910
BtsI	GCAGTG	1 cut(s)	1977
BtsIMutI	CAGTG	4 cut(s)	522, 591, 1977, 2118
BveI	ACCTGC	1 cut(s)	2173
Cac8I	GCNNGC	8 cut(s)	130, 232, 367, 498, 888, 1288, 1818, 2337
CaiI	CAGNNNCTG	1 cut(s)	1445
CciI	TCATGA	2 cut(s)	52, 1942
CfoI	GCGC	4 cut(s)	265, 274, 1131, 1264
Cfr10I	RCCGGY	3 cut(s)	128, 1607, 2588
Cfr13I	GGNCC	7 cut(s)	121, 1571, 1605, 1790, 2525, 2627, 2692
ClaI	ATCGAT	1 cut(s)	810
Csp6I	GTAC	4 cut(s)	224, 934, 2091, 2512
CspAI	ACCGGT	2 cut(s)	1607, 2588
CviAII	CATG	8 cut(s)	53, 77, 703, 1061, 1178, 1259, 1943, 2701
CviQI	GTAC	4 cut(s)	224, 934, 2091, 2512
DdeI	CTNAG	6 cut(s)	201, 639, 1560, 1812, 1881, 2340
DinI	GGCGCC	1 cut(s)	1130
DrdI	GACNNNNNNGTC	2 cut(s)	34, 2327
DseDI	GACNNNNNNGTC	2 cut(s)	34, 2327
EaeI	YGGCCR	1 cut(s)	228
Eam1104I	CTCTTC	5 cut(s)	774, 924, 1413, 1536, 2441
EarI	CTCTTC	5 cut(s)	774, 924, 1413, 1536, 2441
EciI	GGCGGA	3 cut(s)	1010, 1993, 2156
Ecl136II	GAGCTC	1 cut(s)	1423
Eco130I	CCWWGG	3 cut(s)	2134, 2529, 2630
Eco147I	AGGCCT	2 cut(s)	1984, 2230
Eco24I	GRGCYC	1 cut(s)	1425
Eco31I	GGTCTC	1 cut(s)	477
Eco47I	GGWCC	7 cut(s)	121, 1571, 1605, 1790, 2525, 2627, 2692
Eco53kI	GAGCTC	1 cut(s)	1423
Eco57I	CTGAAG	1 cut(s)	1328
Eco72I	CACGTG	1 cut(s)	2282
Eco91I	GGTNACC	1 cut(s)	2362
EcoICRI	GAGCTC	1 cut(s)	1423
EcoNI	CCTNNNNNAGG	1 cut(s)	329
EcoO109I	RGGNCCY	2 cut(s)	2525, 2692
EcoO65I	GGTNACC	1 cut(s)	2362
EcoRII	CCWGG	5 cut(s)	601, 1466, 1632, 1698, 1927
EcoT14I	CCWWGG	3 cut(s)	2134, 2529, 2630
EcoT38I	GRGCYC	1 cut(s)	1425
EgeI	GGCGCC	1 cut(s)	1130
EheI	GGCGCC	1 cut(s)	1130
ErhI	CCWWGG	3 cut(s)	2134, 2529, 2630
Esp3I	CGTCTC	1 cut(s)	204
FaeI	CATG	8 cut(s)	56, 80, 706, 1064, 1181, 1262, 1946, 2704
FalI	AAGNNNNNCTT	6 cut(s)	749, 781, 1239, 1271, 1284, 1316
FaqI	GGGAC	4 cut(s)	190, 362, 461, 647
FatI	CATG	8 cut(s)	52, 76, 702, 1060, 1177, 1258, 1942, 2700
FbaI	TGATCA	3 cut(s)	307, 2115, 2194
FblI	GTMKAC	1 cut(s)	1612
Fnu4HI	GCNGC	7 cut(s)	45, 48, 864, 960, 1098, 1241, 1473
FokI	GGATG	9 cut(s)	80, 671, 872, 968, 1010, 1249, 1649, 2360, 2699
FriOI	GRGCYC	1 cut(s)	1425
Fsp4HI	GCNGC	7 cut(s)	45, 48, 864, 960, 1098, 1241, 1473
FspBI	CTAG	8 cut(s)	159, 666, 713, 891, 1002, 1157, 1287, 2304
GlaI	GCGC	4 cut(s)	264, 273, 1130, 1263
GluI	GCNGC	7 cut(s)	45, 48, 864, 960, 1098, 1241, 1473
GsaI	CCCAGC	2 cut(s)	75, 1746
GsuI	CTGGAG	1 cut(s)	350
HaeII	RGCGCY	1 cut(s)	1132
HaeIII	GGCC	7 cut(s)	230, 324, 500, 785, 1984, 2230, 2635
HapII	CCGG	9 cut(s)	129, 215, 501, 904, 1608, 2332, 2408, 2551, 2589
HhaI	GCGC	4 cut(s)	265, 274, 1131, 1264
Hin1I	GRCGYC	1 cut(s)	1129
Hin1II	CATG	8 cut(s)	56, 80, 706, 1064, 1181, 1262, 1946, 2704
Hin6I	GCGC	4 cut(s)	263, 272, 1129, 1262
HinP1I	GCGC	4 cut(s)	263, 272, 1129, 1262
HincII	GTYRAC	4 cut(s)	899, 1202, 1557, 2073
HindII	GTYRAC	4 cut(s)	899, 1202, 1557, 2073
HindIII	AAGCTT	5 cut(s)	404, 462, 506, 1167, 1340
HpaI	GTTAAC	1 cut(s)	2073
HpaII	CCGG	9 cut(s)	129, 215, 501, 904, 1608, 2332, 2408, 2551, 2589
HphI	GGTGA	7 cut(s)	397, 808, 1676, 2224, 2356, 2467, 2578
Hpy188III	TCNNGA	9 cut(s)	8, 53, 766, 926, 1499, 1737, 1943, 2390, 2469
HpyCH4III	ACNGT	6 cut(s)	1199, 1567, 2001, 2095, 2113, 2509
HpyCH4IV	ACGT	3 cut(s)	1909, 2281, 2322
HpyF3I	CTNAG	6 cut(s)	201, 639, 1560, 1812, 1881, 2340
HpySE526I	ACGT	3 cut(s)	1909, 2281, 2322
Hsp92I	GRCGYC	1 cut(s)	1129
Hsp92II	CATG	8 cut(s)	56, 80, 706, 1064, 1181, 1262, 1946, 2704
HspAI	GCGC	4 cut(s)	263, 272, 1129, 1262
KasI	GGCGCC	1 cut(s)	1128
KroI	GCCGGC	1 cut(s)	128
KroNI	GCCGGC	1 cut(s)	130
Ksp22I	TGATCA	3 cut(s)	307, 2115, 2194
KspAI	GTTAAC	1 cut(s)	2073
LguI	GCTCTTC	1 cut(s)	1413
LmnI	GCTCC	4 cut(s)	198, 1318, 1891, 1927
Lsp1109I	GCAGC	5 cut(s)	31, 946, 1084, 1252, 1484
MaeI	CTAG	8 cut(s)	159, 666, 713, 891, 1002, 1157, 1287, 2304
MaeII	ACGT	3 cut(s)	1909, 2281, 2322
MaeIII	GTNAC	6 cut(s)	1012, 1618, 2307, 2362, 2455, 2584
MfeI	CAATTG	1 cut(s)	1149
MhlI	GDGCHC	5 cut(s)	712, 1177, 1425, 1924, 2422
MlsI	TGGCCA	1 cut(s)	230
MluNI	TGGCCA	1 cut(s)	230
Mly113I	GGCGCC	1 cut(s)	1129
MlyI	GAGTC	5 cut(s)	44, 916, 1120, 1194, 1517
MmeI	TCCRAC	4 cut(s)	372, 1549, 1583, 1817
Mox20I	TGGCCA	1 cut(s)	230
MroNI	GCCGGC	1 cut(s)	128
MscI	TGGCCA	1 cut(s)	230
MseI	TTAA	8 cut(s)	246, 1331, 1404, 1652, 2072, 2097, 2577, 2661
MslI	CAYNNNNRTG	1 cut(s)	1062
Msp20I	TGGCCA	1 cut(s)	230
MspA1I	CMGCKG	2 cut(s)	1243, 1475
MspCI	CTTAAG	2 cut(s)	1330, 1403
MspI	CCGG	9 cut(s)	129, 215, 501, 904, 1608, 2332, 2408, 2551, 2589
MspR9I	CCNGG	8 cut(s)	215, 502, 603, 1468, 1634, 1700, 1929, 2552
MunI	CAATTG	1 cut(s)	1149
Mva1269I	GAATGC	2 cut(s)	2375, 2478
MvaI	CCWGG	5 cut(s)	603, 1468, 1634, 1700, 1929
MvnI	CGCG	1 cut(s)	272
NaeI	GCCGGC	1 cut(s)	130
NarI	GGCGCC	1 cut(s)	1129
NciI	CCSGG	3 cut(s)	215, 502, 2552
NgoMIV	GCCGGC	1 cut(s)	128
NheI	GCTAGC	1 cut(s)	1286
NlaIII	CATG	8 cut(s)	56, 80, 706, 1064, 1181, 1262, 1946, 2704
NlaIV	GGNNCC	6 cut(s)	123, 724, 999, 1130, 1791, 2527
NmuCI	GTSAC	4 cut(s)	1012, 2362, 2455, 2584
NspI	RCATGY	2 cut(s)	80, 1181
NspV	TTCGAA	2 cut(s)	1396, 1867
PagI	TCATGA	2 cut(s)	52, 1942
PceI	AGGCCT	2 cut(s)	1984, 2230
PciI	ACATGT	1 cut(s)	1177
PciSI	GCTCTTC	1 cut(s)	1413
PctI	GAATGC	2 cut(s)	2375, 2478
PdiI	GCCGGC	1 cut(s)	130
PfeI	GAWTC	6 cut(s)	4, 316, 335, 556, 799, 1393
PflMI	CCANNNNNTGG	2 cut(s)	521, 608
PfoI	TCCNGGA	1 cut(s)	1927
PinAI	ACCGGT	2 cut(s)	1607, 2588
PkrI	GCNGC	7 cut(s)	46, 49, 865, 961, 1099, 1242, 1474
PleI	GAGTC	5 cut(s)	43, 916, 1119, 1193, 1516
PluTI	GGCGCC	1 cut(s)	1132
PmaCI	CACGTG	1 cut(s)	2282
PmlI	CACGTG	1 cut(s)	2282
PpsI	GAGTC	5 cut(s)	43, 916, 1119, 1193, 1516
Ppu21I	YACGTR	1 cut(s)	2282
PpuMI	RGGWCCY	2 cut(s)	2525, 2692
PscI	ACATGT	1 cut(s)	1177
Psp124BI	GAGCTC	1 cut(s)	1425
Psp5II	RGGWCCY	2 cut(s)	2525, 2692
Psp6I	CCWGG	5 cut(s)	601, 1466, 1632, 1698, 1927
PspCI	CACGTG	1 cut(s)	2282
PspEI	GGTNACC	1 cut(s)	2362
PspFI	CCCAGC	2 cut(s)	71, 1742
PspGI	CCWGG	5 cut(s)	601, 1466, 1632, 1698, 1927
PspN4I	GGNNCC	6 cut(s)	123, 724, 999, 1130, 1791, 2527
PspPI	GGNCC	7 cut(s)	121, 1571, 1605, 1790, 2525, 2627, 2692
PspPPI	RGGWCCY	2 cut(s)	2525, 2692
PstI	CTGCAG	1 cut(s)	2032
PstNI	CAGNNNCTG	1 cut(s)	1445
PvuII	CAGCTG	2 cut(s)	1243, 1475
RsaI	GTAC	4 cut(s)	225, 935, 2092, 2513
RsaNI	GTAC	4 cut(s)	224, 934, 2091, 2512
RseI	CAYNNNNRTG	1 cut(s)	1062
SacI	GAGCTC	1 cut(s)	1425
SapI	GCTCTTC	1 cut(s)	1413
SaqAI	TTAA	8 cut(s)	246, 1331, 1404, 1652, 2072, 2097, 2577, 2661
SatI	GCNGC	7 cut(s)	45, 48, 864, 960, 1098, 1241, 1473
Sau96I	GGNCC	7 cut(s)	121, 1571, 1605, 1790, 2525, 2627, 2692
ScaI	AGTACT	1 cut(s)	225
SchI	GAGTC	5 cut(s)	44, 916, 1120, 1194, 1517
ScrFI	CCNGG	8 cut(s)	215, 502, 603, 1468, 1634, 1700, 1929, 2552
SduI	GDGCHC	5 cut(s)	712, 1177, 1425, 1924, 2422
SfcI	CTRYAG	3 cut(s)	375, 621, 2028
SfoI	GGCGCC	1 cut(s)	1130
SfuI	TTCGAA	2 cut(s)	1396, 1867
SinI	GGWCC	7 cut(s)	121, 1571, 1605, 1790, 2525, 2627, 2692
SmiMI	CAYNNNNRTG	1 cut(s)	1062
SmlI	CTYRAG	5 cut(s)	562, 1223, 1330, 1403, 2441
SmoI	CTYRAG	5 cut(s)	562, 1223, 1330, 1403, 2441
SseBI	AGGCCT	2 cut(s)	1984, 2230
SsiI	CCGC	7 cut(s)	47, 108, 270, 864, 995, 1978, 2141
SspDI	GGCGCC	1 cut(s)	1128
SspMI	CTAG	8 cut(s)	159, 666, 713, 891, 1002, 1157, 1287, 2304
SstI	GAGCTC	1 cut(s)	1425
StuI	AGGCCT	2 cut(s)	1984, 2230
StyD4I	CCNGG	8 cut(s)	213, 500, 601, 1466, 1632, 1698, 1927, 2550
StyI	CCWWGG	3 cut(s)	2134, 2529, 2630
TaaI	ACNGT	6 cut(s)	1199, 1567, 2001, 2095, 2113, 2509
TaiI	ACGT	3 cut(s)	1912, 2284, 2325
TatI	WGTACW	3 cut(s)	223, 933, 2511
TauI	GCSGC	2 cut(s)	50, 866
TfiI	GAWTC	6 cut(s)	4, 316, 335, 556, 799, 1393
Tru1I	TTAA	8 cut(s)	246, 1331, 1404, 1652, 2072, 2097, 2577, 2661
Tru9I	TTAA	8 cut(s)	246, 1331, 1404, 1652, 2072, 2097, 2577, 2661
TscAI	CASTG	4 cut(s)	522, 591, 1977, 2118
TseFI	GTSAC	4 cut(s)	1012, 2362, 2455, 2584
TseI	GCWGC	5 cut(s)	44, 959, 1097, 1240, 1472
Tsp45I	GTSAC	4 cut(s)	1012, 2362, 2455, 2584
TspRI	CASTG	4 cut(s)	522, 591, 1977, 2118
Van91I	CCANNNNNTGG	2 cut(s)	521, 608
Vha464I	CTTAAG	2 cut(s)	1330, 1403
VneI	GTGCAC	1 cut(s)	1173
VpaK11BI	GGWCC	7 cut(s)	121, 1571, 1605, 1790, 2525, 2627, 2692
XagI	CCTNNNNNAGG	1 cut(s)	329
XapI	RAATTY	4 cut(s)	14, 454, 740, 1071
XceI	RCATGY	2 cut(s)	80, 1181
XcmI	CCANNNNNNNNNTGG	1 cut(s)	827
XmiI	GTMKAC	1 cut(s)	1612
XspI	CTAG	8 cut(s)	159, 666, 713, 891, 1002, 1157, 1287, 2304
ZrmI	AGTACT	1 cut(s)	225

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.