Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7D

Orthogroup ID

OG0002819

Physical Location & Seq

Genomic Coordinates Forward (+)

4141036 .. 4144262

Gene Structure 3227 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7DG053600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2739 bp

ATGAATCCTGACAAATTTACTCACAAGACAAATGAGTCTCTGGCTGGGGCTCATGAACTGGCATCGGATGCTGGCCATGTGCAGTTCACGCCGCTCCATCTTGCAACCGCATTGGTTTCGGACCCTGCTGGCATTTTTCGACAGGCGATTGCCAATGCTAGTGGCAGTGAAGAGGGTCCGAAGTCGGTGGAGAGAGTTTTCAATCAGGCATTGAAGAAGCTCCCGTCTCAGTCCCCTCCCCCGGATCAAGTACCGGCCAGCACTACTCTCATTAAGGCAATTCGCAGAGCGCAGTCGGCGCAGAAAGATCGAGGGGATACACATTTGGCTGTTGATCAGTTGATTCTTGGCCTACTGGAGGATTCGCAGATTGGGGACTTGCTCAAAGAAGCTGGCATTGCTACAGCGAAGGTGAAATCGGAAGTTCAAAAGCTTCGTGGTAAAGAGGGGAAGAAGGTGGAGAGTGCCTCTGGGGACACGAATTTCCAAGCTTTGACTACTTATGGGAGAGACCTTGTTGAGCAGGCCGGGAAGCTTGACCCAGTGATTGGTAGAGATGAGGAGATAAGGAGGGTGGTTCGGATTCTCTCAAGGAGAACGAAGAATAACCCGGTGCTAATTGGAGAGCCAGGAGTTGGGAAAACAGCAGTTGTTGAAGGTTTGGCTCAGAGGATAGTTAGAGGGGATGTCCCTAGTAATCTGGCAGATGTGAGGCTTATCGCATTGGACATGGGTGCTCTAGTAGCTGGTGCCAAGTATAGAGGAGAATTTGAAGAGAGGTTGAAGGCTGTCTTGAAAGAAGTAGAAGAGGCCGAAGGGAAGGTGATTCTGTTTATTGATGAAATCCACCTTGTTCTTGGTGCTGGTAGAACAGAGGGGTCTATGGATGCCGCCAACCTTTTCAAGCCGATGCTCGCTAGAGGTCAACTCCGGTGCATTGGGGCCACGACTCTTGAAGAGTACAGGAAATATGTGGAGAAAGATGCTGCATTTGAGCGAAGGTTTCAACAAGTGTATGTGGCGGAACCTAGTGTTCCAGACACTGTTAGCATCCTTCGAGGCTTGAAGGAGAGATACGAAGGGCATCATGGTGTTCGAATTTTGGATAGAGCTTTAGTTGTTGCTGCACAACTATCGAGTCGATACATCACAGGGCGCCATCTCCCTGACAAGGCAATTGATCTAGTTGATGAAGCTTGTGCAAATGTGAGAGTCCAGCTTGACAGTCAACCAGAAGAAATTGACAACCTTGAGAGAAAGAGGATGCAGCTGGAAGTTGAGCTTCATGCGCTTGAAAAAGAAAAGGATAAAGCTAGCAAAGCTCGTCTTGTTGAGGTTCGGAGGGAGCTTGATGACTTAAGAGACAAGCTTCAGCCCTTGATGATGAAGTATAGAAAAGAGAAGGAAAGGATTGATGAGATTCGAAGGCTTAAGCAAAAGCGTGAAGAGCTCTTATTTGCTTTGACAGAGGCTGAAAGAAGATATGATCTAGCCAGGGCGGCTGATTTGCGTTATGGGGCTATTCAGGAAGTGGAGTCTGCTATTGCCCAGCTTGAAGGAAGCACAGAAGAGAACTTGATGTTGACTGAGACTGTTGGACCAGAACATATTGCAGAGGTTGTAAGCCGTTGGACCGGCATACCAGTAACAAGGCTCGGCCAGGATGACAAAGAGAGGTTAATTGGACTTGGTGAGAGATTGCATAAGAGAGTAGTGGGTCAAAACCAGGCAGTTGAAGCTGTTGCAGAAGCCGTATTGAGATCAAGAGCTGGGTTAGGCAGACCACAACAACCAACTGGATCATTCCTTTTCTTGGGTCCAACTGGTGTGGGTAAAACTGAGCTTGCAAAGGCTCTTGCTGAACAACTTTTTGATGACGAAAATCTAATTGTTCGAATTGATATGTCCGAGTATATGGAGCAACATTCTGTTGCACGTCTGATTGGTGCTCCTCCTGGATATGTTGGTCATGAAGAAGGCGGACAACTCACAGAGGCAGTGAGGCGGAGGCCTTATAGTGTTATACTGTTTGATGAAGTGGAGAAAGCACACCCTGCAGTTTTCAATACTCTCCTGCAAGTTTTAGATGATGGGAGGTTAACTGATGGTCAAGGTCGTACAGTTGATTTTAGGAATACAGTGATCATTATGACTTCAAACCTTGGGGCGGAACATCTCCTAACTGGTTTGTTGGGCAAGTGTTCAATGCAAGTTGCTCGCGATCGAGTTATGCAAGAGGTGAGAACACACTTTAGGCCTGAATTGCTAAATCGGCTAGATGAGGTAGTTGTTTTCGATCCTCTTTCACGTGACCAGCTGAGGAAGGTGGCTAGGTTACAAATGAAGGACGTTGCTGTCCGGCTTGCTGAGAGGGGTGTTGCTTTGGCGGTGACCGATAAAGCATTAGATTATATTCTGGACGAGAGTTATGATCCGGTTTATGGTGCTCGACCTATAAGAAGATGGCTTGAGAAGAGAGTGGTGACAGAGCTATCAAGAATGCTGATTAGAGAAGAGATAGATGAGAACTCAACTGTGTACATAGATGCAGGACCTAAGGGAAATGAGTTGGTTTACCAGGTTGAGAAGAATGGAGGGCTTGTTAATGCCGTCACCGGTCAGAAGTCAGATGTGTTGATACAACTTCCGAATGGACCAAGGCCGACTGATGATGCTCATCAGGCAGTTAAGAAGATGAAGATCGAGGAAATGGATGAGGACCTCGACATGGATGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006417 GO:0006950 GO:0006979 GO:0008150 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009636 GO:0009642 GO:0009644 GO:0009889 GO:0009891 GO:0009893 GO:0009941 GO:0009987 GO:0010035 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031967 GO:0031975 GO:0032268 GO:0032270 GO:0033554 GO:0034248 GO:0034250 GO:0034605 GO:0042221 GO:0042493 GO:0042542 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043335 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045727 GO:0046677 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051716 GO:0060255 GO:0065007 GO:0080090 GO:1901700 GO:2000112

KEGG Pathways

Metabolic & Signaling

ko04213 map04213

Pfam Domains

Protein Families

AAA AAA_2 ClpB_D2-small Clp_N

Protein Analysis

912

Amino Acids

101.61

Weight (kDa)

5.78

Isoelectric Point (pI)

36.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Clp_N	PF02861	5 - 130	9.5e-28	Clp repeat (R) N-terminal domain
AAA_16	PF13191	182 - 233	9.5e-07	AAA ATPase domain
NBD_SMAX1	PF23569	186 - 288	2.2e-09	SMAX1 nucleotide binding domain
AAA	PF00004	205 - 318	3.8e-13	ATPase family associated with various cellular activities (AAA)
AAA_lid_9	PF17871	344 - 444	3.1e-34	AAA lid domain
AAA_2	PF07724	598 - 762	1.1e-68	AAA domain (Cdc48 subfamily)
AAA_22	PF13401	600 - 697	1.8e-06	AAA domain
Sigma54_activat	PF00158	600 - 718	7.7e-06	Sigma-54 interaction domain
AAA_5	PF07728	602 - 721	1.5e-12	AAA domain (dynein-related subfamily)
AAA	PF00004	603 - 738	9.5e-11	ATPase family associated with various cellular activities (AAA)
ClpB_D2-small	PF10431	768 - 847	6.6e-23	C-terminal, D2-small domain, of ClpB protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002819)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74310 AT1G74310 AT4G14670
fragaria_vesca	FvH4_5g14020 FvH4_5g14020 FvH4_5g14020 FvH4_5g14020 FvH4_7g22310 FvH4_7g22310 FvH4_7g22310
malus_domestica	MD06G1201600.v1.1 MD14G1210600.v1.1 MD14G1211000.v1.1
prunus_persica	Prupe.5G203700_v2.0.a1
pyrus_communis	pycom06g17750 pycom14g17400
rosa_chinensis	RchiOBHm_Chr1g0366791 RchiOBHm_Chr7g0182871 RchiOBHm_Chr7g0182901
rosa_laevigata	RLG00000005096 RLG00000005098 RLG00000027319
rosa_multiflora	Rmu_sc0000014.1_g000026 Rmu_sc0000318.1_g000026
rosa_roxburghii	Rroxscaffold_3G00230950 Rroxscaffold_3G00270960
rosa_rugosa	Rorug01G0331900 Rorug01G0333200 Rorug06G0451000
rosa_samantha	Rh1AG340000 Rh7AG054200 Rh7AG403300 Rh7BG053700 Rh7CG055300 Rh7DG053500 Rh7DG053600
rosa_wichuraiana	Rw0G008600 Rw1G030200 Rw7G004420

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	34, 2354
AccB1I	GGYRCC	2 cut(s)	749, 1155
AccB7I	CCANNNNNTGG	2 cut(s)	548, 635
AccBSI	CCGCTC	1 cut(s)	94
AccII	CGCG	1 cut(s)	2220
AciI	CCGC	9 cut(s)	92, 108, 891, 1022, 1499, 1980, 2005, 2168, 2387
AclWI	GGATC	4 cut(s)	252, 1807, 2291, 2426
AcoI	YGGCCR	3 cut(s)	73, 255, 1657
AcsI	RAATTY	4 cut(s)	14, 481, 767, 1098
AcuI	CTGAAG	1 cut(s)	1355
AcvI	CACGTG	1 cut(s)	2309
AcyI	GRCGYC	1 cut(s)	1156
AfaI	GTAC	4 cut(s)	252, 962, 2119, 2540
AfiI	CCNNNNNNNGG	9 cut(s)	241, 358, 548, 635, 1171, 1813, 2167, 2441, 2728
AflII	CTTAAG	2 cut(s)	1357, 1430
AgeI	ACCGGT	1 cut(s)	2615
AjiI	CACGTC	1 cut(s)	1937
AjnI	CCWGG	6 cut(s)	628, 1493, 1659, 1725, 1954, 2577
Alw21I	GWGCWC	4 cut(s)	739, 1452, 1951, 2449
Alw26I	GTCTC	5 cut(s)	42, 231, 504, 1356, 1583
AlwI	GGATC	4 cut(s)	252, 1807, 2291, 2426
AlwNI	CAGNNNCTG	2 cut(s)	1043, 1472
ApeKI	GCWGC	3 cut(s)	986, 1124, 1267
ApoI	RAATTY	4 cut(s)	14, 481, 767, 1098
AsiGI	ACCGGT	1 cut(s)	2615
AspLEI	GCGC	4 cut(s)	292, 301, 1158, 1291
AspS9I	GGNCC	9 cut(s)	121, 176, 942, 1598, 1632, 1817, 2552, 2654, 2719
AsuC2I	CCSGG	3 cut(s)	242, 529, 611
AsuHPI	GGTGA	7 cut(s)	424, 835, 1703, 2251, 2401, 2494, 2605
AsuII	TTCGAA	3 cut(s)	1096, 1423, 1894
AsuNHI	GCTAGC	1 cut(s)	1313
AvaII	GGWCC	8 cut(s)	121, 176, 1598, 1632, 1817, 2552, 2654, 2719
AxyI	CCTNAGG	1 cut(s)	2556
BalI	TGGCCA	1 cut(s)	75
BanI	GGYRCC	2 cut(s)	749, 1155
BanII	GRGCYC	2 cut(s)	52, 1452
BarI	GAAGNNNNNNTAC	2 cut(s)	1058, 1090
BbrPI	CACGTG	1 cut(s)	2309
Bbv12I	GWGCWC	4 cut(s)	739, 1452, 1951, 2449
BbvCI	CCTCAGC	1 cut(s)	2318
BbvI	GCAGC	3 cut(s)	973, 1111, 1279
BccI	CCATC	5 cut(s)	105, 1167, 2084, 2099, 2457
BceAI	ACGGC	3 cut(s)	1611, 1736, 2594
BcgI	CGANNNNNNTGC	8 cut(s)	99, 133, 290, 324, 1116, 1150, 2198, 2232
BciT130I	CCWGG	6 cut(s)	630, 1495, 1661, 1727, 1956, 2579
BciVI	GTATCC	1 cut(s)	310
BclI	TGATCA	2 cut(s)	334, 2142
BcnI	CCSGG	3 cut(s)	242, 529, 611
BcoDI	GTCTC	5 cut(s)	42, 231, 504, 1356, 1583
BfmI	CTRYAG	2 cut(s)	402, 2055
BfoI	RGCGCY	1 cut(s)	1159
BfrI	CTTAAG	2 cut(s)	1357, 1430
BfuI	GTATCC	1 cut(s)	310
BglI	GCCNNNNNGGC	1 cut(s)	1499
BisI	GCNGC	6 cut(s)	92, 891, 987, 1125, 1268, 1500
BlsI	GCNGC	6 cut(s)	93, 892, 988, 1126, 1269, 1501
Bme1390I	CCNGG	9 cut(s)	242, 529, 611, 630, 1495, 1661, 1727, 1956, 2579
Bme18I	GGWCC	8 cut(s)	121, 176, 1598, 1632, 1817, 2552, 2654, 2719
BmgBI	CACGTC	1 cut(s)	1937
BmgT120I	GGNCC	9 cut(s)	121, 176, 942, 1598, 1632, 1817, 2552, 2654, 2719
BmiI	GGNNCC	7 cut(s)	123, 177, 751, 943, 1026, 1157, 1818
BmrFI	CCNGG	9 cut(s)	242, 529, 611, 630, 1495, 1661, 1727, 1956, 2579
BmrI	ACTGGG	1 cut(s)	536
BmtI	GCTAGC	1 cut(s)	1317
BmuI	ACTGGG	1 cut(s)	536
BplI	GAGNNNNNCTC	4 cut(s)	452, 484, 912, 944
BpmI	CTGGAG	1 cut(s)	377
Bpu10I	CCTNAGC	1 cut(s)	2318
Bpu14I	TTCGAA	3 cut(s)	1096, 1423, 1894
BpuEI	CTTGAG	3 cut(s)	574, 1271, 2489
BpuMI	CCSGG	3 cut(s)	242, 529, 611
BsaAI	YACGTR	1 cut(s)	2309
BsaBI	GATNNNNATC	2 cut(s)	2676, 2699
BsaHI	GRCGYC	1 cut(s)	1156
BsaI	GGTCTC	1 cut(s)	504
BsaJI	CCNNGG	4 cut(s)	240, 1494, 2161, 2657
BsaWI	WCCGGW	3 cut(s)	930, 2434, 2615
BsaXI	ACNNNNNCTCC	2 cut(s)	2586, 2616
Bsc4I	CCNNNNNNNGG	9 cut(s)	241, 358, 548, 635, 1171, 1813, 2167, 2441, 2728
Bse118I	RCCGGY	3 cut(s)	253, 1634, 2615
Bse1I	ACTGG	7 cut(s)	63, 360, 542, 1643, 1801, 1828, 2188
Bse21I	CCTNAGG	1 cut(s)	2556
Bse3DI	GCAATG	1 cut(s)	396
Bse8I	GATNNNNATC	2 cut(s)	2676, 2699
BseBI	CCWGG	6 cut(s)	630, 1495, 1661, 1727, 1956, 2579
BseDI	CCNNGG	4 cut(s)	240, 1494, 2161, 2657
BseGI	GGATG	8 cut(s)	73, 691, 892, 1050, 1269, 1669, 2719, 2737
BseJI	GATNNNNATC	2 cut(s)	2676, 2699
BseLI	CCNNNNNNNGG	9 cut(s)	241, 358, 548, 635, 1171, 1813, 2167, 2441, 2728
BseMI	GCAATG	1 cut(s)	396
BseMII	CTCAG	6 cut(s)	242, 680, 1578, 1830, 2309, 2358
BseNI	ACTGG	7 cut(s)	63, 360, 542, 1643, 1801, 1828, 2188
BseRI	GAGGAG	3 cut(s)	575, 777, 1941
BseXI	GCAGC	3 cut(s)	973, 1111, 1279
BseYI	CCCAGC	3 cut(s)	44, 1548, 1769
BsgI	GTGCAG	2 cut(s)	101, 1110
Bsh1236I	CGCG	1 cut(s)	2220
Bsh1285I	CGRYCG	1 cut(s)	2224
BshNI	GGYRCC	2 cut(s)	749, 1155
BshTI	ACCGGT	1 cut(s)	2615
BsiEI	CGRYCG	1 cut(s)	2224
BsiHKAI	GWGCWC	4 cut(s)	739, 1452, 1951, 2449
BsiSI	CCGG	9 cut(s)	242, 254, 528, 611, 931, 1635, 2359, 2435, 2616
BslFI	GGGAC	4 cut(s)	217, 389, 488, 674
BslI	CCNNNNNNNGG	9 cut(s)	241, 358, 548, 635, 1171, 1813, 2167, 2441, 2728
BsmAI	GTCTC	5 cut(s)	42, 231, 504, 1356, 1583
BsmBI	CGTCTC	1 cut(s)	231
BsmFI	GGGAC	4 cut(s)	217, 389, 488, 674
BsmI	GAATGC	1 cut(s)	2505
Bso31I	GGTCTC	1 cut(s)	504
Bsp119I	TTCGAA	3 cut(s)	1096, 1423, 1894
Bsp1286I	GDGCHC	5 cut(s)	52, 739, 1452, 1951, 2449
Bsp1407I	TGTACA	1 cut(s)	2538
Bsp68I	TCGCGA	1 cut(s)	2220
BspACI	CCGC	9 cut(s)	92, 108, 891, 1022, 1499, 1980, 2005, 2168, 2387
BspCNI	CTCAG	6 cut(s)	241, 679, 1579, 1831, 2310, 2359
BspFNI	CGCG	1 cut(s)	2220
BspHI	TCATGA	2 cut(s)	52, 1969
BspLI	GGNNCC	7 cut(s)	123, 177, 751, 943, 1026, 1157, 1818
BspMAI	CTGCAG	1 cut(s)	2059
BspOI	GCTAGC	1 cut(s)	1317
BspPI	GGATC	4 cut(s)	252, 1807, 2291, 2426
BspQI	GCTCTTC	1 cut(s)	1440
BspT104I	TTCGAA	3 cut(s)	1096, 1423, 1894
BspT107I	GGYRCC	2 cut(s)	749, 1155
BspTI	CTTAAG	2 cut(s)	1357, 1430
BspTNI	GGTCTC	1 cut(s)	504
BsrBI	CCGCTC	1 cut(s)	94
BsrDI	GCAATG	1 cut(s)	396
BsrFI	RCCGGY	3 cut(s)	253, 1634, 2615
BsrGI	TGTACA	1 cut(s)	2538
BsrI	ACTGG	7 cut(s)	63, 360, 542, 1643, 1801, 1828, 2188
BssAI	RCCGGY	3 cut(s)	253, 1634, 2615
BssECI	CCNNGG	4 cut(s)	240, 1494, 2161, 2657
BssNI	GRCGYC	1 cut(s)	1156
BssT1I	CCWWGG	2 cut(s)	2161, 2657
Bst2UI	CCWGG	6 cut(s)	630, 1495, 1661, 1727, 1956, 2579
Bst4CI	ACNGT	7 cut(s)	1045, 1226, 1594, 2028, 2122, 2140, 2536
Bst6I	CTCTTC	8 cut(s)	165, 768, 801, 951, 1440, 1563, 2468, 2508
BstACI	GRCGYC	1 cut(s)	1156
BstAFI	CTTAAG	2 cut(s)	1357, 1430
BstAPI	GCANNNNNTGC	2 cut(s)	68, 2054
BstAUI	TGTACA	1 cut(s)	2538
BstBAI	YACGTR	1 cut(s)	2309
BstBI	TTCGAA	3 cut(s)	1096, 1423, 1894
BstDEI	CTNAG	7 cut(s)	228, 666, 1587, 1839, 2318, 2367, 2556
BstEII	GGTNACC	1 cut(s)	2389
BstENI	CCTNNNNNAGG	1 cut(s)	356
BstF5I	GGATG	8 cut(s)	73, 691, 892, 1050, 1269, 1669, 2719, 2737
BstFNI	CGCG	1 cut(s)	2220
BstH2I	RGCGCY	1 cut(s)	1159
BstHHI	GCGC	4 cut(s)	292, 301, 1158, 1291
BstMAI	GTCTC	5 cut(s)	42, 231, 504, 1356, 1583
BstMCI	CGRYCG	1 cut(s)	2224
BstNI	CCWGG	6 cut(s)	630, 1495, 1661, 1727, 1956, 2579
BstPI	GGTNACC	1 cut(s)	2389
BstSCI	CCNGG	9 cut(s)	240, 527, 609, 628, 1493, 1659, 1725, 1954, 2577
BstSFI	CTRYAG	2 cut(s)	402, 2055
BstUI	CGCG	1 cut(s)	2220
BstV1I	GCAGC	3 cut(s)	973, 1111, 1279
Bsu36I	CCTNAGG	1 cut(s)	2556
BsuI	GTATCC	1 cut(s)	310
BtrI	CACGTC	1 cut(s)	1937
BtsCI	GGATG	8 cut(s)	73, 691, 892, 1050, 1269, 1669, 2719, 2737
BtsI	GCAGTG	2 cut(s)	172, 2004
BtsIMutI	CAGTG	5 cut(s)	172, 549, 1041, 2004, 2145
BtuMI	TCGCGA	1 cut(s)	2220
CaiI	CAGNNNCTG	2 cut(s)	1043, 1472
CciI	TCATGA	2 cut(s)	52, 1969
CfoI	GCGC	4 cut(s)	292, 301, 1158, 1291
Cfr10I	RCCGGY	3 cut(s)	253, 1634, 2615
Cfr13I	GGNCC	9 cut(s)	121, 176, 942, 1598, 1632, 1817, 2552, 2654, 2719
CsiI	ACCWGGT	1 cut(s)	2577
Csp6I	GTAC	4 cut(s)	251, 961, 2118, 2539
CspAI	ACCGGT	1 cut(s)	2615
CspCI	CAANNNNNGTGG	6 cut(s)	142, 177, 934, 969, 1809, 1844
CviAII	CATG	7 cut(s)	53, 77, 730, 1088, 1286, 1970, 2728
CviQI	GTAC	4 cut(s)	251, 961, 2118, 2539
DdeI	CTNAG	7 cut(s)	228, 666, 1587, 1839, 2318, 2367, 2556
DinI	GGCGCC	1 cut(s)	1157
DrdI	GACNNNNNNGTC	2 cut(s)	34, 2354
DseDI	GACNNNNNNGTC	2 cut(s)	34, 2354
EaeI	YGGCCR	3 cut(s)	73, 255, 1657
Eam1104I	CTCTTC	8 cut(s)	165, 768, 801, 951, 1440, 1563, 2468, 2508
EarI	CTCTTC	8 cut(s)	165, 768, 801, 951, 1440, 1563, 2468, 2508
EciI	GGCGGA	4 cut(s)	1037, 1995, 2020, 2183
Ecl136II	GAGCTC	1 cut(s)	1450
Eco130I	CCWWGG	2 cut(s)	2161, 2657
Eco147I	AGGCCT	2 cut(s)	2011, 2257
Eco24I	GRGCYC	2 cut(s)	52, 1452
Eco31I	GGTCTC	1 cut(s)	504
Eco47I	GGWCC	8 cut(s)	121, 176, 1598, 1632, 1817, 2552, 2654, 2719
Eco53kI	GAGCTC	1 cut(s)	1450
Eco57I	CTGAAG	1 cut(s)	1355
Eco72I	CACGTG	1 cut(s)	2309
Eco81I	CCTNAGG	1 cut(s)	2556
Eco91I	GGTNACC	1 cut(s)	2389
EcoICRI	GAGCTC	1 cut(s)	1450
EcoNI	CCTNNNNNAGG	1 cut(s)	356
EcoO109I	RGGNCCY	2 cut(s)	2552, 2719
EcoO65I	GGTNACC	1 cut(s)	2389
EcoRII	CCWGG	6 cut(s)	628, 1493, 1659, 1725, 1954, 2577
EcoT14I	CCWWGG	2 cut(s)	2161, 2657
EcoT38I	GRGCYC	2 cut(s)	52, 1452
EgeI	GGCGCC	1 cut(s)	1157
EheI	GGCGCC	1 cut(s)	1157
ErhI	CCWWGG	2 cut(s)	2161, 2657
Esp3I	CGTCTC	1 cut(s)	231
FaeI	CATG	7 cut(s)	56, 80, 733, 1091, 1289, 1973, 2731
FalI	AAGNNNNNCTT	6 cut(s)	776, 808, 1266, 1298, 1311, 1343
FaqI	GGGAC	4 cut(s)	217, 389, 488, 674
FatI	CATG	7 cut(s)	52, 76, 729, 1087, 1285, 1969, 2727
FbaI	TGATCA	2 cut(s)	334, 2142
Fnu4HI	GCNGC	6 cut(s)	92, 891, 987, 1125, 1268, 1500
FokI	GGATG	7 cut(s)	80, 698, 899, 1037, 1276, 1676, 2726
FriOI	GRGCYC	2 cut(s)	52, 1452
Fsp4HI	GCNGC	6 cut(s)	92, 891, 987, 1125, 1268, 1500
GlaI	GCGC	4 cut(s)	291, 300, 1157, 1290
GluI	GCNGC	6 cut(s)	92, 891, 987, 1125, 1268, 1500
GsaI	CCCAGC	3 cut(s)	48, 1552, 1773
GsuI	CTGGAG	1 cut(s)	377
HaeII	RGCGCY	1 cut(s)	1159
HapII	CCGG	9 cut(s)	242, 254, 528, 611, 931, 1635, 2359, 2435, 2616
HhaI	GCGC	4 cut(s)	292, 301, 1158, 1291
Hin1I	GRCGYC	1 cut(s)	1156
Hin1II	CATG	7 cut(s)	56, 80, 733, 1091, 1289, 1973, 2731
Hin6I	GCGC	4 cut(s)	290, 299, 1156, 1289
HinP1I	GCGC	4 cut(s)	290, 299, 1156, 1289
HincII	GTYRAC	4 cut(s)	926, 1229, 1584, 2100
HindII	GTYRAC	4 cut(s)	926, 1229, 1584, 2100
HindIII	AAGCTT	5 cut(s)	431, 489, 533, 1194, 1367
HpaI	GTTAAC	1 cut(s)	2100
HpaII	CCGG	9 cut(s)	242, 254, 528, 611, 931, 1635, 2359, 2435, 2616
HphI	GGTGA	7 cut(s)	424, 835, 1703, 2251, 2401, 2494, 2605
Hpy166II	GTNNAC	7 cut(s)	87, 926, 1229, 1584, 2100, 2539, 2575
Hpy8I	GTNNAC	7 cut(s)	87, 926, 1229, 1584, 2100, 2539, 2575
HpyCH4III	ACNGT	7 cut(s)	1045, 1226, 1594, 2028, 2122, 2140, 2536
HpyCH4IV	ACGT	3 cut(s)	1936, 2308, 2349
HpyF3I	CTNAG	7 cut(s)	228, 666, 1587, 1839, 2318, 2367, 2556
HpySE526I	ACGT	3 cut(s)	1936, 2308, 2349
Hsp92I	GRCGYC	1 cut(s)	1156
Hsp92II	CATG	7 cut(s)	56, 80, 733, 1091, 1289, 1973, 2731
HspAI	GCGC	4 cut(s)	290, 299, 1156, 1289
KasI	GGCGCC	1 cut(s)	1155
Ksp22I	TGATCA	2 cut(s)	334, 2142
KspAI	GTTAAC	1 cut(s)	2100
LguI	GCTCTTC	1 cut(s)	1440
LmnI	GCTCC	5 cut(s)	99, 225, 1345, 1918, 1954
Lsp1109I	GCAGC	3 cut(s)	973, 1111, 1279
MabI	ACCWGGT	1 cut(s)	2577
MaeII	ACGT	3 cut(s)	1936, 2308, 2349
MaeIII	GTNAC	6 cut(s)	1645, 2309, 2334, 2389, 2482, 2611
MbiI	CCGCTC	1 cut(s)	94
MfeI	CAATTG	1 cut(s)	1176
MhlI	GDGCHC	5 cut(s)	52, 739, 1452, 1951, 2449
MlsI	TGGCCA	1 cut(s)	75
MluNI	TGGCCA	1 cut(s)	75
Mly113I	GGCGCC	1 cut(s)	1156
MlyI	GAGTC	5 cut(s)	44, 943, 1147, 1221, 1544
MmeI	TCCRAC	3 cut(s)	1576, 1610, 1844
Mox20I	TGGCCA	1 cut(s)	75
MscI	TGGCCA	1 cut(s)	75
MseI	TTAA	7 cut(s)	273, 1358, 1431, 1679, 2099, 2604, 2688
MslI	CAYNNNNRTG	1 cut(s)	1089
Msp20I	TGGCCA	1 cut(s)	75
MspA1I	CMGCKG	2 cut(s)	1270, 2317
MspCI	CTTAAG	2 cut(s)	1357, 1430
MspI	CCGG	9 cut(s)	242, 254, 528, 611, 931, 1635, 2359, 2435, 2616
MspR9I	CCNGG	9 cut(s)	242, 529, 611, 630, 1495, 1661, 1727, 1956, 2579
MunI	CAATTG	1 cut(s)	1176
Mva1269I	GAATGC	1 cut(s)	2505
MvaI	CCWGG	6 cut(s)	630, 1495, 1661, 1727, 1956, 2579
MvnI	CGCG	1 cut(s)	2220
NarI	GGCGCC	1 cut(s)	1156
NciI	CCSGG	3 cut(s)	242, 529, 611
NheI	GCTAGC	1 cut(s)	1313
NlaIII	CATG	7 cut(s)	56, 80, 733, 1091, 1289, 1973, 2731
NlaIV	GGNNCC	7 cut(s)	123, 177, 751, 943, 1026, 1157, 1818
NmeAIII	GCCGAG	1 cut(s)	1635
NmuCI	GTSAC	4 cut(s)	2309, 2389, 2482, 2611
NruI	TCGCGA	1 cut(s)	2220
NspV	TTCGAA	3 cut(s)	1096, 1423, 1894
PagI	TCATGA	2 cut(s)	52, 1969
PceI	AGGCCT	2 cut(s)	2011, 2257
PciSI	GCTCTTC	1 cut(s)	1440
PctI	GAATGC	1 cut(s)	2505
PfeI	GAWTC	6 cut(s)	4, 343, 362, 583, 826, 1420
PflMI	CCANNNNNTGG	2 cut(s)	548, 635
PfoI	TCCNGGA	1 cut(s)	1954
PinAI	ACCGGT	1 cut(s)	2615
PkrI	GCNGC	6 cut(s)	93, 892, 988, 1126, 1269, 1501
Ple19I	CGATCG	1 cut(s)	2224
PleI	GAGTC	5 cut(s)	43, 943, 1146, 1220, 1543
PluTI	GGCGCC	1 cut(s)	1159
PmaCI	CACGTG	1 cut(s)	2309
PmlI	CACGTG	1 cut(s)	2309
PpsI	GAGTC	5 cut(s)	43, 943, 1146, 1220, 1543
Ppu21I	YACGTR	1 cut(s)	2309
PpuMI	RGGWCCY	2 cut(s)	2552, 2719
Psp124BI	GAGCTC	1 cut(s)	1452
Psp5II	RGGWCCY	2 cut(s)	2552, 2719
Psp6I	CCWGG	6 cut(s)	628, 1493, 1659, 1725, 1954, 2577
PspCI	CACGTG	1 cut(s)	2309
PspEI	GGTNACC	1 cut(s)	2389
PspFI	CCCAGC	3 cut(s)	44, 1548, 1769
PspGI	CCWGG	6 cut(s)	628, 1493, 1659, 1725, 1954, 2577
PspN4I	GGNNCC	7 cut(s)	123, 177, 751, 943, 1026, 1157, 1818
PspPI	GGNCC	9 cut(s)	121, 176, 942, 1598, 1632, 1817, 2552, 2654, 2719
PspPPI	RGGWCCY	2 cut(s)	2552, 2719
PstI	CTGCAG	1 cut(s)	2059
PstNI	CAGNNNCTG	2 cut(s)	1043, 1472
PvuI	CGATCG	1 cut(s)	2224
PvuII	CAGCTG	2 cut(s)	1270, 2317
RruI	TCGCGA	1 cut(s)	2220
RsaI	GTAC	4 cut(s)	252, 962, 2119, 2540
RsaNI	GTAC	4 cut(s)	251, 961, 2118, 2539
RseI	CAYNNNNRTG	1 cut(s)	1089
SacI	GAGCTC	1 cut(s)	1452
SapI	GCTCTTC	1 cut(s)	1440
SaqAI	TTAA	7 cut(s)	273, 1358, 1431, 1679, 2099, 2604, 2688
SatI	GCNGC	6 cut(s)	92, 891, 987, 1125, 1268, 1500
Sau96I	GGNCC	9 cut(s)	121, 176, 942, 1598, 1632, 1817, 2552, 2654, 2719
SchI	GAGTC	5 cut(s)	44, 943, 1147, 1221, 1544
ScrFI	CCNGG	9 cut(s)	242, 529, 611, 630, 1495, 1661, 1727, 1956, 2579
SduI	GDGCHC	5 cut(s)	52, 739, 1452, 1951, 2449
SexAI	ACCWGGT	1 cut(s)	2577
SfcI	CTRYAG	2 cut(s)	402, 2055
SfoI	GGCGCC	1 cut(s)	1157
SfuI	TTCGAA	3 cut(s)	1096, 1423, 1894
SinI	GGWCC	8 cut(s)	121, 176, 1598, 1632, 1817, 2552, 2654, 2719
SmiMI	CAYNNNNRTG	1 cut(s)	1089
SmlI	CTYRAG	5 cut(s)	589, 1250, 1357, 1430, 2468
SmoI	CTYRAG	5 cut(s)	589, 1250, 1357, 1430, 2468
SseBI	AGGCCT	2 cut(s)	2011, 2257
SsiI	CCGC	9 cut(s)	92, 108, 891, 1022, 1499, 1980, 2005, 2168, 2387
SspDI	GGCGCC	1 cut(s)	1155
SstI	GAGCTC	1 cut(s)	1452
StuI	AGGCCT	2 cut(s)	2011, 2257
StyD4I	CCNGG	9 cut(s)	240, 527, 609, 628, 1493, 1659, 1725, 1954, 2577
StyI	CCWWGG	2 cut(s)	2161, 2657
TaaI	ACNGT	7 cut(s)	1045, 1226, 1594, 2028, 2122, 2140, 2536
TaiI	ACGT	3 cut(s)	1939, 2311, 2352
TaqII	GACCGA	1 cut(s)	2408
TatI	WGTACW	2 cut(s)	960, 2538
TauI	GCSGC	3 cut(s)	94, 893, 1502
TfiI	GAWTC	6 cut(s)	4, 343, 362, 583, 826, 1420
Tru1I	TTAA	7 cut(s)	273, 1358, 1431, 1679, 2099, 2604, 2688
Tru9I	TTAA	7 cut(s)	273, 1358, 1431, 1679, 2099, 2604, 2688
TscAI	CASTG	5 cut(s)	172, 549, 1048, 2004, 2145
TseFI	GTSAC	4 cut(s)	2309, 2389, 2482, 2611
TseI	GCWGC	3 cut(s)	986, 1124, 1267
Tsp45I	GTSAC	4 cut(s)	2309, 2389, 2482, 2611
TspRI	CASTG	5 cut(s)	172, 549, 1048, 2004, 2145
Van91I	CCANNNNNTGG	2 cut(s)	548, 635
Vha464I	CTTAAG	2 cut(s)	1357, 1430
VpaK11BI	GGWCC	8 cut(s)	121, 176, 1598, 1632, 1817, 2552, 2654, 2719
XagI	CCTNNNNNAGG	1 cut(s)	356
XapI	RAATTY	4 cut(s)	14, 481, 767, 1098
XcmI	CCANNNNNNNNNTGG	1 cut(s)	854

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.