Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7D

Orthogroup ID

OG0002819

Physical Location & Seq

Genomic Coordinates Forward (+)

4119889 .. 4123027

Gene Structure 3139 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7DG053500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2652 bp

ATGAATCCTGACAAATTTACTCACAAGACAAATGAGTCTCTGGATGGGGCTCATGAACTGGCATCGGATGCTGGCCATGTGCACTTCACGCCGCTCCATCTTGCAACCGCATTGGTTTCGGACCCTGCTGGCATTTTTCGACAGGCGATTGCCAATGCTAGTGGCAGTGAAGAGGGTCCGAAGTCGGTGGAGAGAGTTTTCAATCAGGCATTGAAGAAGGTTCCGTCTCAGTCCCCTCCCCCGGATCAAGTACCGGCCAGCACTACTCTCATTAAGGCAATTCGGAGAGCGCAGTCGGCGCAGAAAGATCGAGGGGATACACATTTGGCTGTTGATCAGTTGATTCTTGGCCTACTGGAGGATTCGCAGATTGGGGACTTGCTCAAAGAAGCTGGCATTGCTACAGCGAAGGTGAAATCGGAAGTTCAAAAGCTTCGTGGTAAAGAGGGGAAGAAGGTGGAGAGTGCCTCTGGGGACACGAATTTCCAAGCTTTGACTACTTATGGGAGAGACCTTGTTGAGCAGGCCGAGAAGATTGACCCAGTGATTGGTAGAGATGAGGAGATAAGGAGGGTGGTTCGGATTCTCTCAAGGAGAACGAAGAATAACCCGGTGCTAATTGGAGAGCCAGGAGTTGGGAAAACAGCAGTAGTTGAAGGTTTGGCTCAGAGGATAGCAAGAGGGGATGTTCCTAGTAATCTGGCAGATGTGAGGCTTATCGCATTGGACATGGGTGCTCTAGTAGCTGGTGCCAAGTATAGAGGAGAATTTGAGGAGAGGTTGAAGGCTGTCTTGAAAGAAGTAGAAGAGGCCGAAGGGAAGGTGAATCTGTTTATTGATGAAATCCACCTTGTTCTTGGTGCTGGTAGAACAGAGGGGTCTATGGATGCCGCCAACCTTTTCAAGCCAATGCTTGCTAGAGGTACACTCCGGTGCATTGGGGCAACGACTCTTGAAGAGTACAGGAAATATGTGGAGAAGGATGCTGCATTTGAGCGAAGGTTTCTACAAGTTCATGTGGCGGAACCTAGTGTGCCAGACACTGCTAGCATCCTTCGAGGCTTGAAGGAGAAATATGAAGGGTATCATAAAGTTAGAATTTTGGATACAGCTTTTGTTGTTGCTGCACAACTATCGAATCAATATATCACAGGGCGCCATCTCCCTGACAAGGCAATTGATCTAGTTGATGAAGCTTGTGCAAATGTGAGAGTCCAGCTTGACAGTCAACCAGAAGAAATTGACAACCTTGAGAGAAAGAGGATGCAGCTGGAAGTTGAGCGTCATGCGCTTGAAAAAGAAGAGGATAAAGCTAACGAAGCTCGTCTTCTTGAAGTTCGGAGGGAGCTTGATGACTTAAGAGACAAGCTTCAGCCCTTGATGAAGAAATATAGAAAAGAGAAGGAAATTCGAAGGCTTAAGAAGGAAATTCGAAGGCTTAAGGAAAAGCGTAAAGATCTCTTAATTGATTTGACAGAGGTTGAAAGAAGATGTGATCTAGCCAGGGCGGCTGATTTGCGTTATGGGGCTATTCAGGAAGTGGAGTCTGCTATTGCCCAGCTTGAAGGAAGCACAGAAGAGAACTTGATGTTGACTGAGACTGTTGGACCAGAACATATTGCAGAGGTTGTAAGCCGTTGGACCGGCATACCAGTAACAAGGCTCGCCCAGGATGACAAAGAGAGGTTAATTGGACTTGGTGAGAGATTGCATAAGAGAGTAGTGGGCCAAAACCAGGCAGTTGATGCTGTTGCAGAAGCCGTATTGAGATCAAGAGCTGGGTTAGGCAGACCACAACAACCAACTGGATCATTCCTTTTCTTGGGTCCAACTGGTGTTGGTAAAACTGAGCTTGCAAAGGCTCTTGCTGAACAACTTTTTGATGACGAAAATCTAATTGTTCGAATTGATATGTCTGAGTATATGGAGCAACATTCTGTTGCACGTCTGATTGGTGCTCCTCCTGGATATGTTGGTCATGAAGACGGTGGACAACTCACAGAGGCAGTGAGGCGGAGGCCTTATAGTGTTATACTGTTTGATGAAGTGGAGAAAGCACACCCTCCAGTTTTCAATACTCTCCTGCAAGTTTTAGATGATGGGAGGTTAACTGATGGTCAAGGTCGTACAGTTGATTTTAGGAATACAGTGATCATTATGACTTCAAACCTTGGGGCGGAACATCTCCTAACTGTTTCGTTGGGCAAGTGTTCAATGCAAGATGCTCATGATCCAGTTATGCAAGAGGTGAGAAAATACTTTAGGCCTGAATTGCTAAATCGGTTAGATGAGGTAGTTTTTTTCGATCCTCTTTCACCTGAGCAACTGAGGAAGGTGGCTAGGTTACGAATGGATGACGTTGATGCCCGGCTTGCTAAGAGGGGTGTTGCTTTTGCGGTGACTGATGAAGCATTAGATTATATTGTGCACGAGAGTTCTGATCCGGTTTATGGTGCTCGACCTATAAGAAGATGGCTTGAGAAGAGAGTGGTGACAGAGCTATCAAGAATGCTGATTAGAGAAGAGATAGATGAGAACTCAACTGTGTACATAGATGCAGGACCTATGGCAAATGAGTTGGTTTACCGGGTTGAGAAGAATAGAGGGCTTGTTAATGCTGTCACCGGTCAGAACTCAGATGTGTTGATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006417 GO:0006950 GO:0006979 GO:0008150 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009636 GO:0009642 GO:0009644 GO:0009889 GO:0009891 GO:0009893 GO:0009941 GO:0009987 GO:0010035 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031967 GO:0031975 GO:0032268 GO:0032270 GO:0033554 GO:0034248 GO:0034250 GO:0034605 GO:0042221 GO:0042493 GO:0042542 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043335 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045727 GO:0046677 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051716 GO:0060255 GO:0065007 GO:0080090 GO:1901700 GO:2000112

KEGG Pathways

Metabolic & Signaling

ko04213 map04213

Pfam Domains

Protein Families

AAA AAA_2 ClpB_D2-small Clp_N

Protein Analysis

883

Amino Acids

98.57

Weight (kDa)

5.85

Isoelectric Point (pI)

38.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Clp_N	PF02861	5 - 130	1.3e-26	Clp repeat (R) N-terminal domain
AAA_16	PF13191	182 - 228	9.1e-07	AAA ATPase domain
NBD_SMAX1	PF23569	186 - 271	1.2e-08	SMAX1 nucleotide binding domain
AAA	PF00004	205 - 317	3.1e-11	ATPase family associated with various cellular activities (AAA)
AAA_lid_9	PF17871	345 - 443	3.4e-32	AAA lid domain
AAA_2	PF07724	601 - 765	2.7e-68	AAA domain (Cdc48 subfamily)
AAA_22	PF13401	603 - 700	1.1e-06	AAA domain
AAA_5	PF07728	605 - 724	5e-12	AAA domain (dynein-related subfamily)
AAA	PF00004	606 - 724	4.3e-09	ATPase family associated with various cellular activities (AAA)
ClpB_D2-small	PF10431	771 - 850	9.2e-21	C-terminal, D2-small domain, of ClpB protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002819)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74310 AT1G74310 AT4G14670
fragaria_vesca	FvH4_5g14020 FvH4_5g14020 FvH4_5g14020 FvH4_5g14020 FvH4_7g22310 FvH4_7g22310 FvH4_7g22310
malus_domestica	MD06G1201600.v1.1 MD14G1210600.v1.1 MD14G1211000.v1.1
prunus_persica	Prupe.5G203700_v2.0.a1
pyrus_communis	pycom06g17750 pycom14g17400
rosa_chinensis	RchiOBHm_Chr1g0366791 RchiOBHm_Chr7g0182871 RchiOBHm_Chr7g0182901
rosa_laevigata	RLG00000005096 RLG00000005098 RLG00000027319
rosa_multiflora	Rmu_sc0000014.1_g000026 Rmu_sc0000318.1_g000026
rosa_roxburghii	Rroxscaffold_3G00230950 Rroxscaffold_3G00270960
rosa_rugosa	Rorug01G0331900 Rorug01G0333200 Rorug06G0451000
rosa_samantha	Rh1AG340000 Rh7AG054200 Rh7AG403300 Rh7BG053700 Rh7CG055300 Rh7DG053500 Rh7DG053600
rosa_wichuraiana	Rw0G008600 Rw1G030200 Rw7G004420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	34
AccB1I	GGYRCC	2 cut(s)	749, 1155
AccB7I	CCANNNNNTGG	2 cut(s)	548, 635
AccBSI	CCGCTC	1 cut(s)	94
AciI	CCGC	8 cut(s)	92, 108, 891, 1022, 1508, 2014, 2177, 2396
AclWI	GGATC	5 cut(s)	252, 1816, 2225, 2300, 2435
AcoI	YGGCCR	2 cut(s)	73, 255
AcsI	RAATTY	6 cut(s)	14, 481, 767, 1098, 1407, 1428
AcuI	CTGAAG	1 cut(s)	1355
AcyI	GRCGYC	1 cut(s)	1156
AfaI	GTAC	5 cut(s)	252, 925, 962, 2128, 2549
AfiI	CCNNNNNNNGG	9 cut(s)	241, 358, 548, 635, 1171, 1735, 1822, 2176, 2450
AflII	CTTAAG	3 cut(s)	1357, 1418, 1439
AgeI	ACCGGT	1 cut(s)	2624
AjiI	CACGTC	1 cut(s)	1946
AjnI	CCWGG	5 cut(s)	628, 1502, 1668, 1734, 1963
AleI	CACNNNNGTG	1 cut(s)	931
Alw21I	GWGCWC	5 cut(s)	84, 739, 1960, 2430, 2458
Alw26I	GTCTC	5 cut(s)	42, 231, 504, 1356, 1592
Alw44I	GTGCAC	2 cut(s)	80, 2426
AlwI	GGATC	5 cut(s)	252, 1816, 2225, 2300, 2435
AlwNI	CAGNNNCTG	1 cut(s)	1043
AoxI	GGCC	8 cut(s)	73, 255, 349, 525, 810, 1726, 2018, 2264
ApaLI	GTGCAC	2 cut(s)	80, 2426
ApeKI	GCWGC	3 cut(s)	986, 1124, 1267
ApoI	RAATTY	6 cut(s)	14, 481, 767, 1098, 1407, 1428
AsiGI	ACCGGT	1 cut(s)	2624
AspLEI	GCGC	4 cut(s)	292, 301, 1158, 1291
AspS9I	GGNCC	7 cut(s)	121, 176, 1607, 1641, 1726, 1826, 2561
AsuC2I	CCSGG	4 cut(s)	242, 611, 2368, 2588
AsuHPI	GGTGA	8 cut(s)	424, 835, 1712, 2260, 2307, 2410, 2503, 2614
AsuII	TTCGAA	3 cut(s)	1411, 1432, 1903
AsuNHI	GCTAGC	1 cut(s)	1046
AvaII	GGWCC	6 cut(s)	121, 176, 1607, 1641, 1826, 2561
BaeGI	GKGCMC	2 cut(s)	84, 2430
BalI	TGGCCA	1 cut(s)	75
BanI	GGYRCC	2 cut(s)	749, 1155
BanII	GRGCYC	1 cut(s)	52
BauI	CACGAG	1 cut(s)	2429
BbsI	GAAGAC	2 cut(s)	1319, 1989
Bbv12I	GWGCWC	5 cut(s)	84, 739, 1960, 2430, 2458
BbvI	GCAGC	3 cut(s)	973, 1111, 1279
BccI	CCATC	6 cut(s)	38, 105, 1167, 2093, 2108, 2466
BceAI	ACGGC	2 cut(s)	1620, 1745
BcgI	CGANNNNNNTGC	6 cut(s)	99, 133, 290, 324, 1116, 1150
BciT130I	CCWGG	5 cut(s)	630, 1504, 1670, 1736, 1965
BciVI	GTATCC	2 cut(s)	310, 1099
BclI	TGATCA	2 cut(s)	334, 2151
BcnI	CCSGG	4 cut(s)	242, 611, 2368, 2588
BcoDI	GTCTC	5 cut(s)	42, 231, 504, 1356, 1592
BfaI	CTAG	9 cut(s)	159, 693, 740, 918, 1029, 1047, 1184, 1499, 2340
BfmI	CTRYAG	1 cut(s)	402
BfoI	RGCGCY	1 cut(s)	1159
BfrI	CTTAAG	3 cut(s)	1357, 1418, 1439
BfuI	GTATCC	2 cut(s)	310, 1099
BglI	GCCNNNNNGGC	1 cut(s)	1508
BglII	AGATCT	1 cut(s)	1456
BisI	GCNGC	6 cut(s)	92, 891, 987, 1125, 1268, 1509
BlsI	GCNGC	6 cut(s)	93, 892, 988, 1126, 1269, 1510
Bme1390I	CCNGG	9 cut(s)	242, 611, 630, 1504, 1670, 1736, 1965, 2368, 2588
Bme18I	GGWCC	6 cut(s)	121, 176, 1607, 1641, 1826, 2561
BmgBI	CACGTC	1 cut(s)	1946
BmgT120I	GGNCC	7 cut(s)	121, 176, 1607, 1641, 1726, 1826, 2561
BmiI	GGNNCC	7 cut(s)	123, 177, 222, 751, 1026, 1157, 1827
BmrFI	CCNGG	9 cut(s)	242, 611, 630, 1504, 1670, 1736, 1965, 2368, 2588
BmrI	ACTGGG	1 cut(s)	536
BmtI	GCTAGC	1 cut(s)	1050
BmuI	ACTGGG	1 cut(s)	536
BpiI	GAAGAC	2 cut(s)	1319, 1989
BplI	GAGNNNNNCTC	4 cut(s)	452, 484, 912, 944
BpmI	CTGGAG	2 cut(s)	377, 2049
Bpu10I	CCTNAGC	1 cut(s)	2319
Bpu14I	TTCGAA	3 cut(s)	1411, 1432, 1903
BpuEI	CTTGAG	3 cut(s)	574, 1271, 2498
BpuMI	CCSGG	4 cut(s)	242, 611, 2368, 2588
BsaHI	GRCGYC	1 cut(s)	1156
BsaI	GGTCTC	1 cut(s)	504
BsaJI	CCNNGG	4 cut(s)	240, 1503, 1668, 2170
BsaWI	WCCGGW	3 cut(s)	930, 2443, 2624
Bsc4I	CCNNNNNNNGG	9 cut(s)	241, 358, 548, 635, 1171, 1735, 1822, 2176, 2450
Bse118I	RCCGGY	3 cut(s)	253, 1643, 2624
Bse1I	ACTGG	8 cut(s)	63, 360, 542, 1652, 1810, 1837, 2066, 2234
Bse3DI	GCAATG	1 cut(s)	396
BseBI	CCWGG	5 cut(s)	630, 1504, 1670, 1736, 1965
BseDI	CCNNGG	4 cut(s)	240, 1503, 1668, 2170
BseGI	GGATG	9 cut(s)	49, 73, 691, 892, 988, 1050, 1269, 1678, 2359
BseLI	CCNNNNNNNGG	9 cut(s)	241, 358, 548, 635, 1171, 1735, 1822, 2176, 2450
BseMI	GCAATG	1 cut(s)	396
BseMII	CTCAG	8 cut(s)	242, 680, 1587, 1839, 1908, 2310, 2318, 2649
BseNI	ACTGG	8 cut(s)	63, 360, 542, 1652, 1810, 1837, 2066, 2234
BseRI	GAGGAG	4 cut(s)	575, 777, 788, 1950
BseSI	GKGCMC	2 cut(s)	84, 2430
BseXI	GCAGC	3 cut(s)	973, 1111, 1279
BseYI	CCCAGC	2 cut(s)	1557, 1778
BsgI	GTGCAG	1 cut(s)	1110
BshFI	GGCC	8 cut(s)	75, 257, 351, 527, 812, 1728, 2020, 2266
BshNI	GGYRCC	2 cut(s)	749, 1155
BshTI	ACCGGT	1 cut(s)	2624
BsiHKAI	GWGCWC	5 cut(s)	84, 739, 1960, 2430, 2458
BsiSI	CCGG	9 cut(s)	242, 254, 611, 931, 1644, 2368, 2444, 2587, 2625
BslFI	GGGAC	3 cut(s)	217, 389, 488
BslI	CCNNNNNNNGG	9 cut(s)	241, 358, 548, 635, 1171, 1735, 1822, 2176, 2450
BsmAI	GTCTC	5 cut(s)	42, 231, 504, 1356, 1592
BsmBI	CGTCTC	1 cut(s)	231
BsmFI	GGGAC	3 cut(s)	217, 389, 488
BsmI	GAATGC	1 cut(s)	2514
BsnI	GGCC	8 cut(s)	75, 257, 351, 527, 812, 1728, 2020, 2266
Bso31I	GGTCTC	1 cut(s)	504
Bsp119I	TTCGAA	3 cut(s)	1411, 1432, 1903
Bsp1286I	GDGCHC	6 cut(s)	52, 84, 739, 1960, 2430, 2458
Bsp1407I	TGTACA	1 cut(s)	2547
BspACI	CCGC	8 cut(s)	92, 108, 891, 1022, 1508, 2014, 2177, 2396
BspANI	GGCC	8 cut(s)	75, 257, 351, 527, 812, 1728, 2020, 2266
BspCNI	CTCAG	8 cut(s)	241, 679, 1588, 1840, 1909, 2311, 2319, 2648
BspHI	TCATGA	3 cut(s)	52, 1978, 2227
BspLI	GGNNCC	7 cut(s)	123, 177, 222, 751, 1026, 1157, 1827
BspOI	GCTAGC	1 cut(s)	1050
BspPI	GGATC	5 cut(s)	252, 1816, 2225, 2300, 2435
BspT104I	TTCGAA	3 cut(s)	1411, 1432, 1903
BspT107I	GGYRCC	2 cut(s)	749, 1155
BspTI	CTTAAG	3 cut(s)	1357, 1418, 1439
BspTNI	GGTCTC	1 cut(s)	504
BsrBI	CCGCTC	1 cut(s)	94
BsrDI	GCAATG	1 cut(s)	396
BsrFI	RCCGGY	3 cut(s)	253, 1643, 2624
BsrGI	TGTACA	1 cut(s)	2547
BsrI	ACTGG	8 cut(s)	63, 360, 542, 1652, 1810, 1837, 2066, 2234
BssAI	RCCGGY	3 cut(s)	253, 1643, 2624
BssECI	CCNNGG	4 cut(s)	240, 1503, 1668, 2170
BssNI	GRCGYC	1 cut(s)	1156
BssSI	CACGAG	1 cut(s)	2429
BssT1I	CCWWGG	1 cut(s)	2170
Bst2BI	CACGAG	1 cut(s)	2429
Bst2UI	CCWGG	5 cut(s)	630, 1504, 1670, 1736, 1965
Bst4CI	ACNGT	8 cut(s)	1226, 1603, 1988, 2037, 2131, 2149, 2194, 2545
Bst6I	CTCTTC	7 cut(s)	165, 801, 951, 1296, 1572, 2477, 2517
BstACI	GRCGYC	1 cut(s)	1156
BstAFI	CTTAAG	3 cut(s)	1357, 1418, 1439
BstAPI	GCANNNNNTGC	2 cut(s)	68, 1745
BstAUI	TGTACA	1 cut(s)	2547
BstBI	TTCGAA	3 cut(s)	1411, 1432, 1903
BstDEI	CTNAG	9 cut(s)	228, 666, 1596, 1848, 1917, 2319, 2327, 2376, 2635
BstENI	CCTNNNNNAGG	1 cut(s)	356
BstF5I	GGATG	9 cut(s)	49, 73, 691, 892, 988, 1050, 1269, 1678, 2359
BstH2I	RGCGCY	1 cut(s)	1159
BstHHI	GCGC	4 cut(s)	292, 301, 1158, 1291
BstMAI	GTCTC	5 cut(s)	42, 231, 504, 1356, 1592
BstNI	CCWGG	5 cut(s)	630, 1504, 1670, 1736, 1965
BstSCI	CCNGG	9 cut(s)	240, 609, 628, 1502, 1668, 1734, 1963, 2366, 2586
BstSFI	CTRYAG	1 cut(s)	402
BstSLI	GKGCMC	2 cut(s)	84, 2430
BstV1I	GCAGC	3 cut(s)	973, 1111, 1279
BstV2I	GAAGAC	2 cut(s)	1319, 1989
BstX2I	RGATCY	1 cut(s)	1456
BstYI	RGATCY	1 cut(s)	1456
BsuI	GTATCC	2 cut(s)	310, 1099
BsuRI	GGCC	8 cut(s)	75, 257, 351, 527, 812, 1728, 2020, 2266
BtrI	CACGTC	1 cut(s)	1946
BtsCI	GGATG	9 cut(s)	49, 73, 691, 892, 988, 1050, 1269, 1678, 2359
BtsI	GCAGTG	3 cut(s)	172, 1041, 2013
BtsIMutI	CAGTG	5 cut(s)	172, 549, 1041, 2013, 2154
CaiI	CAGNNNCTG	1 cut(s)	1043
CciI	TCATGA	3 cut(s)	52, 1978, 2227
CfoI	GCGC	4 cut(s)	292, 301, 1158, 1291
Cfr10I	RCCGGY	3 cut(s)	253, 1643, 2624
Cfr13I	GGNCC	7 cut(s)	121, 176, 1607, 1641, 1726, 1826, 2561
CseI	GACGC	1 cut(s)	1271
Csp6I	GTAC	5 cut(s)	251, 924, 961, 2127, 2548
CspAI	ACCGGT	1 cut(s)	2624
CspCI	CAANNNNNGTGG	2 cut(s)	142, 177
CviAII	CATG	7 cut(s)	53, 77, 730, 1016, 1286, 1979, 2228
CviQI	GTAC	5 cut(s)	251, 924, 961, 2127, 2548
DdeI	CTNAG	9 cut(s)	228, 666, 1596, 1848, 1917, 2319, 2327, 2376, 2635
DinI	GGCGCC	1 cut(s)	1157
DrdI	GACNNNNNNGTC	1 cut(s)	34
DseDI	GACNNNNNNGTC	1 cut(s)	34
EaeI	YGGCCR	2 cut(s)	73, 255
Eam1104I	CTCTTC	7 cut(s)	165, 801, 951, 1296, 1572, 2477, 2517
EarI	CTCTTC	7 cut(s)	165, 801, 951, 1296, 1572, 2477, 2517
EciI	GGCGGA	3 cut(s)	1037, 2029, 2192
Eco130I	CCWWGG	1 cut(s)	2170
Eco147I	AGGCCT	2 cut(s)	2020, 2266
Eco24I	GRGCYC	1 cut(s)	52
Eco31I	GGTCTC	1 cut(s)	504
Eco47I	GGWCC	6 cut(s)	121, 176, 1607, 1641, 1826, 2561
Eco57I	CTGAAG	1 cut(s)	1355
EcoNI	CCTNNNNNAGG	1 cut(s)	356
EcoO109I	RGGNCCY	1 cut(s)	2561
EcoRII	CCWGG	5 cut(s)	628, 1502, 1668, 1734, 1963
EcoT14I	CCWWGG	1 cut(s)	2170
EcoT38I	GRGCYC	1 cut(s)	52
EgeI	GGCGCC	1 cut(s)	1157
EheI	GGCGCC	1 cut(s)	1157
ErhI	CCWWGG	1 cut(s)	2170
Esp3I	CGTCTC	1 cut(s)	231
FaeI	CATG	7 cut(s)	56, 80, 733, 1019, 1289, 1982, 2231
FalI	AAGNNNNNCTT	4 cut(s)	776, 808, 1311, 1343
FaqI	GGGAC	3 cut(s)	217, 389, 488
FatI	CATG	7 cut(s)	52, 76, 729, 1015, 1285, 1978, 2227
FbaI	TGATCA	2 cut(s)	334, 2151
Fnu4HI	GCNGC	6 cut(s)	92, 891, 987, 1125, 1268, 1509
FokI	GGATG	9 cut(s)	56, 80, 698, 899, 995, 1037, 1276, 1685, 2366
FriOI	GRGCYC	1 cut(s)	52
Fsp4HI	GCNGC	6 cut(s)	92, 891, 987, 1125, 1268, 1509
FspBI	CTAG	9 cut(s)	159, 693, 740, 918, 1029, 1047, 1184, 1499, 2340
GlaI	GCGC	4 cut(s)	291, 300, 1157, 1290
GluI	GCNGC	6 cut(s)	92, 891, 987, 1125, 1268, 1509
GsaI	CCCAGC	2 cut(s)	1561, 1782
GsuI	CTGGAG	2 cut(s)	377, 2049
HaeII	RGCGCY	1 cut(s)	1159
HaeIII	GGCC	8 cut(s)	75, 257, 351, 527, 812, 1728, 2020, 2266
HapII	CCGG	9 cut(s)	242, 254, 611, 931, 1644, 2368, 2444, 2587, 2625
HgaI	GACGC	1 cut(s)	1271
HhaI	GCGC	4 cut(s)	292, 301, 1158, 1291
Hin1I	GRCGYC	1 cut(s)	1156
Hin1II	CATG	7 cut(s)	56, 80, 733, 1019, 1289, 1982, 2231
Hin6I	GCGC	4 cut(s)	290, 299, 1156, 1289
HinP1I	GCGC	4 cut(s)	290, 299, 1156, 1289
HincII	GTYRAC	3 cut(s)	1229, 1593, 2109
HindII	GTYRAC	3 cut(s)	1229, 1593, 2109
HindIII	AAGCTT	4 cut(s)	431, 489, 1194, 1367
HpaI	GTTAAC	1 cut(s)	2109
HpaII	CCGG	9 cut(s)	242, 254, 611, 931, 1644, 2368, 2444, 2587, 2625
HphI	GGTGA	8 cut(s)	424, 835, 1712, 2260, 2307, 2410, 2503, 2614
Hpy166II	GTNNAC	9 cut(s)	82, 926, 1229, 1593, 1991, 2109, 2428, 2548, 2584
Hpy8I	GTNNAC	9 cut(s)	82, 926, 1229, 1593, 1991, 2109, 2428, 2548, 2584
HpyCH4III	ACNGT	8 cut(s)	1226, 1603, 1988, 2037, 2131, 2149, 2194, 2545
HpyCH4IV	ACGT	2 cut(s)	1945, 2358
HpyF3I	CTNAG	9 cut(s)	228, 666, 1596, 1848, 1917, 2319, 2327, 2376, 2635
HpySE526I	ACGT	2 cut(s)	1945, 2358
Hsp92I	GRCGYC	1 cut(s)	1156
Hsp92II	CATG	7 cut(s)	56, 80, 733, 1019, 1289, 1982, 2231
HspAI	GCGC	4 cut(s)	290, 299, 1156, 1289
KasI	GGCGCC	1 cut(s)	1155
Ksp22I	TGATCA	2 cut(s)	334, 2151
KspAI	GTTAAC	1 cut(s)	2109
LmnI	GCTCC	4 cut(s)	99, 1345, 1927, 1963
Lsp1109I	GCAGC	3 cut(s)	973, 1111, 1279
MaeI	CTAG	9 cut(s)	159, 693, 740, 918, 1029, 1047, 1184, 1499, 2340
MaeII	ACGT	2 cut(s)	1945, 2358
MaeIII	GTNAC	5 cut(s)	1654, 2343, 2398, 2491, 2620
MbiI	CCGCTC	1 cut(s)	94
MfeI	CAATTG	1 cut(s)	1176
MflI	RGATCY	1 cut(s)	1456
MhlI	GDGCHC	6 cut(s)	52, 84, 739, 1960, 2430, 2458
MlsI	TGGCCA	1 cut(s)	75
MluNI	TGGCCA	1 cut(s)	75
Mly113I	GGCGCC	1 cut(s)	1156
MlyI	GAGTC	4 cut(s)	44, 943, 1221, 1553
MmeI	TCCRAC	3 cut(s)	1585, 1619, 1853
Mox20I	TGGCCA	1 cut(s)	75
MscI	TGGCCA	1 cut(s)	75
MseI	TTAA	8 cut(s)	273, 1358, 1419, 1440, 1463, 1688, 2108, 2613
MslI	CAYNNNNRTG	1 cut(s)	931
Msp20I	TGGCCA	1 cut(s)	75
MspA1I	CMGCKG	1 cut(s)	1270
MspCI	CTTAAG	3 cut(s)	1357, 1418, 1439
MspI	CCGG	9 cut(s)	242, 254, 611, 931, 1644, 2368, 2444, 2587, 2625
MspR9I	CCNGG	9 cut(s)	242, 611, 630, 1504, 1670, 1736, 1965, 2368, 2588
MunI	CAATTG	1 cut(s)	1176
Mva1269I	GAATGC	1 cut(s)	2514
MvaI	CCWGG	5 cut(s)	630, 1504, 1670, 1736, 1965
NarI	GGCGCC	1 cut(s)	1156
NciI	CCSGG	4 cut(s)	242, 611, 2368, 2588
NheI	GCTAGC	1 cut(s)	1046
NlaIII	CATG	7 cut(s)	56, 80, 733, 1019, 1289, 1982, 2231
NlaIV	GGNNCC	7 cut(s)	123, 177, 222, 751, 1026, 1157, 1827
NmeAIII	GCCGAG	1 cut(s)	553
NmuCI	GTSAC	3 cut(s)	2398, 2491, 2620
NspV	TTCGAA	3 cut(s)	1411, 1432, 1903
OliI	CACNNNNGTG	1 cut(s)	931
PagI	TCATGA	3 cut(s)	52, 1978, 2227
PceI	AGGCCT	2 cut(s)	2020, 2266
PctI	GAATGC	1 cut(s)	2514
PfeI	GAWTC	6 cut(s)	4, 343, 362, 583, 826, 1138
PflMI	CCANNNNNTGG	2 cut(s)	548, 635
PfoI	TCCNGGA	1 cut(s)	1963
PinAI	ACCGGT	1 cut(s)	2624
PkrI	GCNGC	6 cut(s)	93, 892, 988, 1126, 1269, 1510
PleI	GAGTC	4 cut(s)	43, 943, 1220, 1552
PluTI	GGCGCC	1 cut(s)	1159
PpsI	GAGTC	4 cut(s)	43, 943, 1220, 1552
PpuMI	RGGWCCY	1 cut(s)	2561
Psp5II	RGGWCCY	1 cut(s)	2561
Psp6I	CCWGG	5 cut(s)	628, 1502, 1668, 1734, 1963
PspFI	CCCAGC	2 cut(s)	1557, 1778
PspGI	CCWGG	5 cut(s)	628, 1502, 1668, 1734, 1963
PspN4I	GGNNCC	7 cut(s)	123, 177, 222, 751, 1026, 1157, 1827
PspPI	GGNCC	7 cut(s)	121, 176, 1607, 1641, 1726, 1826, 2561
PspPPI	RGGWCCY	1 cut(s)	2561
PstNI	CAGNNNCTG	1 cut(s)	1043
PsuI	RGATCY	1 cut(s)	1456
PvuII	CAGCTG	1 cut(s)	1270
RsaI	GTAC	5 cut(s)	252, 925, 962, 2128, 2549
RsaNI	GTAC	5 cut(s)	251, 924, 961, 2127, 2548
RseI	CAYNNNNRTG	1 cut(s)	931
SaqAI	TTAA	8 cut(s)	273, 1358, 1419, 1440, 1463, 1688, 2108, 2613
SatI	GCNGC	6 cut(s)	92, 891, 987, 1125, 1268, 1509
Sau96I	GGNCC	7 cut(s)	121, 176, 1607, 1641, 1726, 1826, 2561
SchI	GAGTC	4 cut(s)	44, 943, 1221, 1553
ScrFI	CCNGG	9 cut(s)	242, 611, 630, 1504, 1670, 1736, 1965, 2368, 2588
SduI	GDGCHC	6 cut(s)	52, 84, 739, 1960, 2430, 2458
SfcI	CTRYAG	1 cut(s)	402
SfoI	GGCGCC	1 cut(s)	1157
SfuI	TTCGAA	3 cut(s)	1411, 1432, 1903
SinI	GGWCC	6 cut(s)	121, 176, 1607, 1641, 1826, 2561
SmiMI	CAYNNNNRTG	1 cut(s)	931
SmlI	CTYRAG	6 cut(s)	589, 1250, 1357, 1418, 1439, 2477
SmoI	CTYRAG	6 cut(s)	589, 1250, 1357, 1418, 1439, 2477
SseBI	AGGCCT	2 cut(s)	2020, 2266
SsiI	CCGC	8 cut(s)	92, 108, 891, 1022, 1508, 2014, 2177, 2396
SspDI	GGCGCC	1 cut(s)	1155
SspMI	CTAG	9 cut(s)	159, 693, 740, 918, 1029, 1047, 1184, 1499, 2340
StuI	AGGCCT	2 cut(s)	2020, 2266
StyD4I	CCNGG	9 cut(s)	240, 609, 628, 1502, 1668, 1734, 1963, 2366, 2586
StyI	CCWWGG	1 cut(s)	2170
TaaI	ACNGT	8 cut(s)	1226, 1603, 1988, 2037, 2131, 2149, 2194, 2545
TaiI	ACGT	2 cut(s)	1948, 2361
TaqI	TCGA	9 cut(s)	139, 310, 1057, 1136, 1411, 1432, 1903, 2304, 2458
TatI	WGTACW	2 cut(s)	960, 2547
TauI	GCSGC	3 cut(s)	94, 893, 1511
TfiI	GAWTC	6 cut(s)	4, 343, 362, 583, 826, 1138
Tru1I	TTAA	8 cut(s)	273, 1358, 1419, 1440, 1463, 1688, 2108, 2613
Tru9I	TTAA	8 cut(s)	273, 1358, 1419, 1440, 1463, 1688, 2108, 2613
TscAI	CASTG	5 cut(s)	172, 549, 1048, 2013, 2154
TseFI	GTSAC	3 cut(s)	2398, 2491, 2620
TseI	GCWGC	3 cut(s)	986, 1124, 1267
Tsp45I	GTSAC	3 cut(s)	2398, 2491, 2620
TspGWI	ACGGA	1 cut(s)	213
TspRI	CASTG	5 cut(s)	172, 549, 1048, 2013, 2154
Van91I	CCANNNNNTGG	2 cut(s)	548, 635
Vha464I	CTTAAG	3 cut(s)	1357, 1418, 1439
VneI	GTGCAC	2 cut(s)	80, 2426
VpaK11BI	GGWCC	6 cut(s)	121, 176, 1607, 1641, 1826, 2561
XagI	CCTNNNNNAGG	1 cut(s)	356
XapI	RAATTY	6 cut(s)	14, 481, 767, 1098, 1407, 1428
XcmI	CCANNNNNNNNNTGG	1 cut(s)	854
XspI	CTAG	9 cut(s)	159, 693, 740, 918, 1029, 1047, 1184, 1499, 2340

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.