Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0002819

Physical Location & Seq

Genomic Coordinates Forward (+)

29624025 .. 29628301

Gene Structure 4277 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1210600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2739 bp

ATGAATCCTGATAAGTTTACCCACAAGACCAATGAGACTATTGCCGGAGCTCATGGCCTGGCCATGGATGCTGGCCACGCGCAGTTCACGCCGCTCCATCTTGCAACAACATTGGTATCTGATCCCAGTGGCATTTTCAGCCAAGCCATTGCCAATGCCGGTGGCAGTGCAGACGCTCCGAAGTCTGTGGAGAGGGTGTTCAACCAAGCCCTGAAGAAGCTCCCATCTCAGTCACCTCCGCCGGATGAAATCCCAGCTAGTTCATCTCTCATCAAAGTGATTAGGAGAGCGCAGTCAGCGCAGAAGGCGAGAGGGGACACACACTTGGCCGTTGATCAGTTGATTCTTGGTCTTCTTGAGGATTCCCAGATTGGGGACTTGTTGAAGGAAGCCGGCATTGCCACGGCCAGAGTGAAATCTGAAGTCGAAAAGCTTCGTGGGAAGGAAGGGAAGAAGGTGGAGAGTGCTTCTGGCGATACTACATTTCAGGCATTGAAGACTTATGGACGGGACCTTGTTGAGCAGGCCGGGAAACTTGACCCGGTTATTGGCCGTGACGAGGAGATAAGGAGAGTGGTGAGGATTCTTTCGAGGAGGACTAAGAACAACCCTGTTCTAATTGGAGAGCCGGGAGTTGGGAAGACTGCTGTGGTTGAAGGGCTGGCTCAGAGGATAGTCGGTGGGGATATCCCTAGTAATCTGGCCGATGTGAGGCTCATTGCATTGGATATGGGAGCTTTGGTGGCCGGGGCCAAGTATCGAGGGGAGTTTGAGGAGCGGTTAAAGGCGGTTTTGAAGGAAGTGGAAGAGGCTGAGGGGAAGGTGATTCTGTTTATTGATGAAATTCACCTTGTTCTAGGCGCCGGAAGGACGGAGGGATCCATGGATGCAGCCAACCTGTTCAAGCCAATGCTCGCGCGGGGGCAACTTCGGTGCATTGGCGCAACAACTCTTGAGGAGTACAGAAAATATGTAGAGAAGGATGCGGCATTTGAGAGAAGGTTCCAGCAGGTTTATGTGGCGGAGCCTAGTGTTCCGGATACAATTAGCATCCTTCGAGGTTTGAAAGAGAAGTATGAGGGTCATCATGGTGTTCGGATTCTGGACAGAGCTCTTGTTGTTGCTGCACAGCTTTCGAGTCGATACATCACAGGGCGACATCTCCCTGACAAGGCGATTGATCTAGTTGACGAAGCTTGTGCGAATGTGAGAGTCCAACTTGACAGCCAACCTGAAGAAATCGATAACCTCGAAAGGAAGAGGATGCAGCTGGAAATTGAGCTTCATGCACTTGAGAGAGAAAAGGACAAAGCTAGCAAAGCTCGTCTTGTCGAGGTGCGAAAAGAGCTTGATGAACTGAGAGACAAGCTTCAGCCACTGGTGATGAAGTATCGAAAGGAGAAGGAAAGGGTTGATGAGCTTCGAAAGCTTAAGCAGAAGCGTGAAGAGCTCTTGGTAGCTTTAGCAGAGGCCGAAAGAAGATATGATTTGGCTAGGGCTGCTGATTTGCGTTATGGCGCTATTCAGGAAGTGGAAACCACCATTGCAAAGCTTGAAGGCAGTACCGACGAAAACTTGATTTTGACAGAGACTGTTGGACCAGAACAAATTGCAGAGGTGGTAAGCCGTTGGACCGGCATACCTGTCACACGGCTGGGCCAGAATGACAAAGAAAGGTTAATTGGACTTGCGGAAAGGTTGCATAAGAGAGTAGTGGGGCAAGACCAGGCAGTTGATGCTGTCGCCGAGGCAGTTTTAAGGTCAAGAGCGGGGCTAGGAAGGCCACAACAACCTACTGGTTCCTTCCTCTTCTTGGGTCCAACTGGTGTCGGTAAAACCGAGCTTGCGAAGGCGCTTGCTGAACAACTTTTTGATGACGAGAATCTGATTGTCAGAATTGACATGTCCGAGTATATGGAACAACATTCTGTCTCGCGCTTAATTGGCGCTCCCCCTGGGTATATTGGTCACGATGAAGGCGGGCAACTCACGGAGGCCGTGAGGCGGAGGCCTTACAGTGTTGTATTGTTTGATGAAGTGGAGAAGGCTCACACCGCCGTTTTCAACACTCTCCTGCAAGTCCTGGATGATGGAAGACTGACCGATGGACAAGGCCGGACAGTTGATTTCAGAAACACGGTGATCATCATGACTTCAAACTTGGGAGCAGAACATCTCCTCTCCGGTTTGATGGGCAAGTGTTCGATGCAGGTTGCTCGCGATAAAGTTATGCAACAGGTGAGAAAGCACTTTAGGCCGGAGCTGCTGAATCGTCTCGATGAGATCGTTGTATTCGATCCTCTTTCTCACGACCAACTGAGGAAGGTTGCCAGATTGCAAATGAAAGATGTTGCAGCCCGACTGGCCGAGAGGGGCATCGCTATGGCAGTCACCGATGCAGCGTTGGAATACATTCTGGACGAGAGTTATGATCCCGTTTACGGTGCCAGACCTATTCGGAGATGGCTGGAGAAGAAGGTGGTGACGGAGTTGTCGCGGATGCTTGTGCGAGAAGAAATAGATGAAAACTCGACCGTGTTCATAGATGCAGCGCCAACCGGAAGCGGGCTGGTGTATAGGGTTGAGAAGAACGGAGGGCTTGTCAATGCGGCGACCGGGCAGAAGTCGGATGTGTTGATCCACATCCCGAACACCAACGGGCCGAGGTCTGATGCCGAACAGGCTGTGAAGAAGATGAAGATTGAGGAAATGGATGAAGATCTTGACATGGATGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006417 GO:0006950 GO:0006979 GO:0008150 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009636 GO:0009642 GO:0009644 GO:0009889 GO:0009891 GO:0009893 GO:0009941 GO:0009987 GO:0010035 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031967 GO:0031975 GO:0032268 GO:0032270 GO:0033554 GO:0034248 GO:0034250 GO:0034605 GO:0042221 GO:0042493 GO:0042542 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043335 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045727 GO:0046677 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051716 GO:0060255 GO:0065007 GO:0080090 GO:1901700 GO:2000112

KEGG Pathways

Metabolic & Signaling

ko04213 map04213

Pfam Domains

Protein Families

AAA AAA_2 ClpB_D2-small Clp_N

Protein Analysis

913

Amino Acids

101.03

Weight (kDa)

5.88

Isoelectric Point (pI)

35.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Clp_N	PF02861	5 - 130	5.6e-28	Clp repeat (R) N-terminal domain
AAA_16	PF13191	182 - 228	2e-06	AAA ATPase domain
NBD_SMAX1	PF23569	186 - 288	4.4e-08	SMAX1 nucleotide binding domain
AAA	PF00004	205 - 318	3.6e-13	ATPase family associated with various cellular activities (AAA)
AAA_lid_9	PF17871	344 - 445	1.6e-34	AAA lid domain
AAA_2	PF07724	598 - 762	1.2e-68	AAA domain (Cdc48 subfamily)
Sigma54_activat	PF00158	601 - 718	6.3e-06	Sigma-54 interaction domain
AAA_5	PF07728	602 - 721	6.5e-12	AAA domain (dynein-related subfamily)
AAA	PF00004	603 - 738	2.5e-10	ATPase family associated with various cellular activities (AAA)
ClpB_D2-small	PF10431	768 - 847	7.3e-23	C-terminal, D2-small domain, of ClpB protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002819)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74310 AT1G74310 AT4G14670
fragaria_vesca	FvH4_5g14020 FvH4_5g14020 FvH4_5g14020 FvH4_5g14020 FvH4_7g22310 FvH4_7g22310 FvH4_7g22310
malus_domestica	MD06G1201600.v1.1 MD14G1210600.v1.1 MD14G1211000.v1.1
prunus_persica	Prupe.5G203700_v2.0.a1
pyrus_communis	pycom06g17750 pycom14g17400
rosa_chinensis	RchiOBHm_Chr1g0366791 RchiOBHm_Chr7g0182871 RchiOBHm_Chr7g0182901
rosa_laevigata	RLG00000005096 RLG00000005098 RLG00000027319
rosa_multiflora	Rmu_sc0000014.1_g000026 Rmu_sc0000318.1_g000026
rosa_roxburghii	Rroxscaffold_3G00230950 Rroxscaffold_3G00270960
rosa_rugosa	Rorug01G0331900 Rorug01G0333200 Rorug06G0451000
rosa_samantha	Rh1AG340000 Rh7AG054200 Rh7AG403300 Rh7BG053700 Rh7CG055300 Rh7DG053500 Rh7DG053600
rosa_wichuraiana	Rw0G008600 Rw1G030200 Rw7G004420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2491
Acc36I	ACCTGC	2 cut(s)	1000, 2200
AccB1I	GGYRCC	2 cut(s)	860, 2444
AccBSI	CCGCTC	3 cut(s)	94, 778, 1769
AccII	CGCG	6 cut(s)	80, 917, 919, 1936, 2220, 2497
AccIII	TCCGGA	1 cut(s)	1036
AclWI	GGATC	6 cut(s)	116, 873, 886, 2291, 2426, 2632
AcoI	YGGCCR	8 cut(s)	60, 73, 327, 405, 550, 702, 744, 2364
AcsI	RAATTY	1 cut(s)	843
AcuI	CTGAAG	4 cut(s)	233, 441, 1254, 1355
AcyI	GRCGYC	1 cut(s)	861
AfaI	GTAC	2 cut(s)	962, 1564
AflII	CTTAAG	1 cut(s)	1430
AflIII	ACRYGT	1 cut(s)	1902
AjnI	CCWGG	4 cut(s)	57, 1725, 1954, 2082
AjuI	GAANNNNNNNTTGG	2 cut(s)	1665, 1697
AloI	GAACNNNNNNTCC	2 cut(s)	182, 214
Alw21I	GWGCWC	3 cut(s)	52, 1114, 1452
Alw26I	GTCTC	5 cut(s)	29, 1356, 1583, 1936, 2279
AlwI	GGATC	6 cut(s)	116, 873, 886, 2291, 2426, 2632
AlwNI	CAGNNNCTG	2 cut(s)	1378, 1592
Aor13HI	TCCGGA	1 cut(s)	1036
ApeKI	GCWGC	8 cut(s)	890, 1124, 1267, 1499, 2263, 2354, 2399, 2549
ApoI	RAATTY	1 cut(s)	843
Asp700I	GAANNNNTTC	2 cut(s)	432, 2412
AspS9I	GGNCC	7 cut(s)	511, 750, 1598, 1632, 1657, 1817, 2660
AsuC2I	CCSGG	5 cut(s)	529, 542, 630, 748, 2617
AsuHPI	GGTGA	9 cut(s)	225, 589, 835, 839, 1393, 2152, 2251, 2383, 2494
AsuII	TTCGAA	1 cut(s)	1423
AsuNHI	GCTAGC	1 cut(s)	1313
AvaII	GGWCC	4 cut(s)	511, 1598, 1632, 1817
BaeI	ACNNNNGTAYC	2 cut(s)	99, 132
BalI	TGGCCA	2 cut(s)	62, 75
BamHI	GGATCC	1 cut(s)	878
BanI	GGYRCC	2 cut(s)	860, 2444
BanII	GRGCYC	3 cut(s)	52, 1114, 1452
BbsI	GAAGAC	4 cut(s)	344, 503, 647, 2101
Bbv12I	GWGCWC	3 cut(s)	52, 1114, 1452
BbvCI	CCTCAGC	1 cut(s)	813
BbvI	GCAGC	8 cut(s)	902, 1111, 1279, 1486, 2250, 2366, 2411, 2561
BccI	CCATC	6 cut(s)	105, 232, 2084, 2099, 2185, 2457
BceAI	ACGGC	7 cut(s)	314, 420, 537, 1611, 1667, 1982, 2042
BcgI	CGANNNNNNTGC	6 cut(s)	1116, 1150, 1181, 1215, 2198, 2232
BciT130I	CCWGG	4 cut(s)	59, 1727, 1956, 2084
BciVI	GTATCC	1 cut(s)	1033
BclI	TGATCA	2 cut(s)	334, 2142
BcnI	CCSGG	5 cut(s)	529, 542, 630, 748, 2617
BcoDI	GTCTC	5 cut(s)	29, 1356, 1583, 1936, 2279
BfaI	CTAG	8 cut(s)	258, 693, 857, 1029, 1184, 1314, 1494, 1775
BfoI	RGCGCY	5 cut(s)	864, 1521, 1856, 1950, 2555
BfrI	CTTAAG	1 cut(s)	1430
BfuAI	ACCTGC	2 cut(s)	1000, 2200
BfuI	GTATCC	1 cut(s)	1033
BglI	GCCNNNNNGGC	2 cut(s)	2363, 2681
BglII	AGATCT	1 cut(s)	2719
Bme1390I	CCNGG	9 cut(s)	59, 529, 542, 630, 748, 1727, 1956, 2084, 2617
Bme18I	GGWCC	4 cut(s)	511, 1598, 1632, 1817
BmgT120I	GGNCC	7 cut(s)	511, 750, 1598, 1632, 1657, 1817, 2660
BmiI	GGNNCC	9 cut(s)	512, 751, 862, 880, 1004, 1026, 1801, 1818, 2446
BmrFI	CCNGG	9 cut(s)	59, 529, 542, 630, 748, 1727, 1956, 2084, 2617
BmrI	ACTGGG	1 cut(s)	120
BmtI	GCTAGC	1 cut(s)	1317
BmuI	ACTGGG	1 cut(s)	120
BpiI	GAAGAC	4 cut(s)	344, 503, 647, 2101
BpmI	CTGGAG	1 cut(s)	2489
Bpu10I	CCTNAGC	1 cut(s)	813
Bpu14I	TTCGAA	1 cut(s)	1423
BpuEI	CTTGAG	3 cut(s)	377, 974, 1313
BpuMI	CCSGG	5 cut(s)	529, 542, 630, 748, 2617
Bsa29I	ATCGAT	1 cut(s)	1242
BsaBI	GATNNNNATC	2 cut(s)	2642, 2718
BsaHI	GRCGYC	1 cut(s)	861
BsaJI	CCNNGG	8 cut(s)	63, 402, 747, 882, 1746, 1954, 1955, 2663
BsaWI	WCCGGW	3 cut(s)	1036, 2183, 2558
BsaXI	ACNNNNNCTCC	6 cut(s)	182, 212, 726, 756, 2586, 2616
Bse118I	RCCGGY	3 cut(s)	158, 392, 1634
Bse1I	ACTGG	5 cut(s)	126, 1383, 1801, 1828, 2367
Bse3DI	GCAATG	4 cut(s)	147, 396, 717, 1542
Bse8I	GATNNNNATC	2 cut(s)	2642, 2718
BseAI	TCCGGA	1 cut(s)	1036
BseBI	CCWGG	4 cut(s)	59, 1727, 1956, 2084
BseCI	ATCGAT	1 cut(s)	1242
BseDI	CCNNGG	8 cut(s)	63, 402, 747, 882, 1746, 1954, 1955, 2663
BseJI	GATNNNNATC	2 cut(s)	2642, 2718
BseMI	GCAATG	4 cut(s)	147, 396, 717, 1542
BseMII	CTCAG	5 cut(s)	242, 680, 804, 1349, 2309
BseNI	ACTGG	5 cut(s)	126, 1383, 1801, 1828, 2367
BseRI	GAGGAG	5 cut(s)	575, 607, 788, 971, 2168
BseXI	GCAGC	8 cut(s)	902, 1111, 1279, 1486, 2250, 2366, 2411, 2561
BseYI	CCCAGC	2 cut(s)	253, 1654
BsgI	GTGCAG	2 cut(s)	189, 1110
Bsh1236I	CGCG	6 cut(s)	80, 917, 919, 1936, 2220, 2497
Bsh1285I	CGRYCG	2 cut(s)	2535, 2616
BshNI	GGYRCC	2 cut(s)	860, 2444
BshVI	ATCGAT	1 cut(s)	1242
BsiEI	CGRYCG	2 cut(s)	2535, 2616
BsiHKAI	GWGCWC	3 cut(s)	52, 1114, 1452
BslFI	GGGAC	3 cut(s)	329, 389, 524
BsmAI	GTCTC	5 cut(s)	29, 1356, 1583, 1936, 2279
BsmBI	CGTCTC	1 cut(s)	2279
BsmFI	GGGAC	3 cut(s)	329, 389, 524
Bsp119I	TTCGAA	1 cut(s)	1423
Bsp1286I	GDGCHC	3 cut(s)	52, 1114, 1452
Bsp13I	TCCGGA	1 cut(s)	1036
Bsp19I	CCATGG	2 cut(s)	63, 882
Bsp68I	TCGCGA	1 cut(s)	2220
BspCNI	CTCAG	5 cut(s)	241, 679, 805, 1350, 2310
BspDI	ATCGAT	1 cut(s)	1242
BspEI	TCCGGA	1 cut(s)	1036
BspFNI	CGCG	6 cut(s)	80, 917, 919, 1936, 2220, 2497
BspHI	TCATGA	1 cut(s)	2148
BspLI	GGNNCC	9 cut(s)	512, 751, 862, 880, 1004, 1026, 1801, 1818, 2446
BspMI	ACCTGC	2 cut(s)	1000, 2200
BspOI	GCTAGC	1 cut(s)	1317
BspPI	GGATC	6 cut(s)	116, 873, 886, 2291, 2426, 2632
BspQI	GCTCTTC	1 cut(s)	1440
BspT104I	TTCGAA	1 cut(s)	1423
BspT107I	GGYRCC	2 cut(s)	860, 2444
BspTI	CTTAAG	1 cut(s)	1430
BsrBI	CCGCTC	3 cut(s)	94, 778, 1769
BsrDI	GCAATG	4 cut(s)	147, 396, 717, 1542
BsrFI	RCCGGY	3 cut(s)	158, 392, 1634
BsrI	ACTGG	5 cut(s)	126, 1383, 1801, 1828, 2367
BssAI	RCCGGY	3 cut(s)	158, 392, 1634
BssECI	CCNNGG	8 cut(s)	63, 402, 747, 882, 1746, 1954, 1955, 2663
BssNI	GRCGYC	1 cut(s)	861
BssT1I	CCWWGG	2 cut(s)	63, 882
Bst2UI	CCWGG	4 cut(s)	59, 1727, 1956, 2084
Bst4CI	ACNGT	6 cut(s)	1594, 2018, 2122, 2140, 2444, 2536
Bst6I	CTCTTC	4 cut(s)	801, 1253, 1440, 1814
BstACI	GRCGYC	1 cut(s)	861
BstAFI	CTTAAG	1 cut(s)	1430
BstAPI	GCANNNNNTGC	1 cut(s)	1736
BstBI	TTCGAA	1 cut(s)	1423
BstDEI	CTNAG	6 cut(s)	228, 600, 666, 813, 1358, 2318
BstDSI	CCRYGG	3 cut(s)	63, 402, 882
BstFNI	CGCG	6 cut(s)	80, 917, 919, 1936, 2220, 2497
BstH2I	RGCGCY	5 cut(s)	864, 1521, 1856, 1950, 2555
BstMAI	GTCTC	5 cut(s)	29, 1356, 1583, 1936, 2279
BstMCI	CGRYCG	2 cut(s)	2535, 2616
BstNI	CCWGG	4 cut(s)	59, 1727, 1956, 2084
BstNSI	RCATGY	1 cut(s)	1906
BstSCI	CCNGG	9 cut(s)	57, 527, 540, 628, 746, 1725, 1954, 2082, 2615
BstUI	CGCG	6 cut(s)	80, 917, 919, 1936, 2220, 2497
BstV1I	GCAGC	8 cut(s)	902, 1111, 1279, 1486, 2250, 2366, 2411, 2561
BstV2I	GAAGAC	4 cut(s)	344, 503, 647, 2101
BstX2I	RGATCY	2 cut(s)	878, 2719
BstYI	RGATCY	2 cut(s)	878, 2719
Bsu15I	ATCGAT	1 cut(s)	1242
BsuI	GTATCC	1 cut(s)	1033
BsuTUI	ATCGAT	1 cut(s)	1242
BtgI	CCRYGG	3 cut(s)	63, 402, 882
BtgZI	GCGATG	1 cut(s)	2362
BtsI	GCAGTG	1 cut(s)	172
BtsIMutI	CAGTG	4 cut(s)	133, 172, 1376, 2023
BtuMI	TCGCGA	1 cut(s)	2220
BveI	ACCTGC	2 cut(s)	1000, 2200
CaiI	CAGNNNCTG	2 cut(s)	1378, 1592
CciI	TCATGA	1 cut(s)	2148
Cfr10I	RCCGGY	3 cut(s)	158, 392, 1634
Cfr13I	GGNCC	7 cut(s)	511, 750, 1598, 1632, 1657, 1817, 2660
ClaI	ATCGAT	1 cut(s)	1242
CseI	GACGC	1 cut(s)	182
Csp6I	GTAC	2 cut(s)	961, 1563
CspCI	CAANNNNNGTGG	2 cut(s)	142, 177
CviAII	CATG	8 cut(s)	53, 64, 883, 1088, 1286, 1903, 2149, 2728
CviQI	GTAC	2 cut(s)	961, 1563
DdeI	CTNAG	6 cut(s)	228, 600, 666, 813, 1358, 2318
DinI	GGCGCC	1 cut(s)	862
DrdI	GACNNNNNNGTC	1 cut(s)	2491
DseDI	GACNNNNNNGTC	1 cut(s)	2491
EaeI	YGGCCR	8 cut(s)	60, 73, 327, 405, 550, 702, 744, 2364
Eam1104I	CTCTTC	4 cut(s)	801, 1253, 1440, 1814
EarI	CTCTTC	4 cut(s)	801, 1253, 1440, 1814
EciI	GGCGGA	3 cut(s)	228, 1037, 2020
Ecl136II	GAGCTC	3 cut(s)	50, 1112, 1450
Eco130I	CCWWGG	2 cut(s)	63, 882
Eco147I	AGGCCT	1 cut(s)	2011
Eco24I	GRGCYC	3 cut(s)	52, 1114, 1452
Eco32I	GATATC	1 cut(s)	688
Eco47I	GGWCC	4 cut(s)	511, 1598, 1632, 1817
Eco53kI	GAGCTC	3 cut(s)	50, 1112, 1450
Eco57I	CTGAAG	4 cut(s)	233, 441, 1254, 1355
EcoICRI	GAGCTC	3 cut(s)	50, 1112, 1450
EcoO109I	RGGNCCY	1 cut(s)	511
EcoRII	CCWGG	4 cut(s)	57, 1725, 1954, 2082
EcoRV	GATATC	1 cut(s)	688
EcoT14I	CCWWGG	2 cut(s)	63, 882
EcoT38I	GRGCYC	3 cut(s)	52, 1114, 1452
EgeI	GGCGCC	1 cut(s)	862
EheI	GGCGCC	1 cut(s)	862
ErhI	CCWWGG	2 cut(s)	63, 882
Esp3I	CGTCTC	1 cut(s)	2279
FaeI	CATG	8 cut(s)	56, 67, 886, 1091, 1289, 1906, 2152, 2731
FalI	AAGNNNNNCTT	2 cut(s)	1311, 1343
FaqI	GGGAC	3 cut(s)	329, 389, 524
FatI	CATG	8 cut(s)	52, 63, 882, 1087, 1285, 1902, 2148, 2727
FauI	CCCGC	4 cut(s)	912, 1762, 1973, 2558
FbaI	TGATCA	2 cut(s)	334, 2142
FriOI	GRGCYC	3 cut(s)	52, 1114, 1452
FspBI	CTAG	8 cut(s)	258, 693, 857, 1029, 1184, 1314, 1494, 1775
GsaI	CCCAGC	2 cut(s)	257, 1658
GsuI	CTGGAG	1 cut(s)	2489
HaeII	RGCGCY	5 cut(s)	864, 1521, 1856, 1950, 2555
HgaI	GACGC	1 cut(s)	182
Hin1I	GRCGYC	1 cut(s)	861
Hin1II	CATG	8 cut(s)	56, 67, 886, 1091, 1289, 1906, 2152, 2731
HincII	GTYRAC	1 cut(s)	1189
HindII	GTYRAC	1 cut(s)	1189
HindIII	AAGCTT	5 cut(s)	431, 1194, 1367, 1427, 1550
HphI	GGTGA	9 cut(s)	225, 589, 835, 839, 1393, 2152, 2251, 2383, 2494
Hpy166II	GTNNAC	4 cut(s)	18, 87, 1189, 2440
Hpy8I	GTNNAC	4 cut(s)	18, 87, 1189, 2440
Hpy99I	CGWCG	1 cut(s)	1571
HpyCH4III	ACNGT	6 cut(s)	1594, 2018, 2122, 2140, 2444, 2536
HpyF3I	CTNAG	6 cut(s)	228, 600, 666, 813, 1358, 2318
Hsp92I	GRCGYC	1 cut(s)	861
Hsp92II	CATG	8 cut(s)	56, 67, 886, 1091, 1289, 1906, 2152, 2731
KasI	GGCGCC	1 cut(s)	860
Kpn2I	TCCGGA	1 cut(s)	1036
KroI	GCCGGC	1 cut(s)	392
KroNI	GCCGGC	1 cut(s)	394
Ksp22I	TGATCA	2 cut(s)	334, 2142
LguI	GCTCTTC	1 cut(s)	1440
Lsp1109I	GCAGC	8 cut(s)	902, 1111, 1279, 1486, 2250, 2366, 2411, 2561
MaeI	CTAG	8 cut(s)	258, 693, 857, 1029, 1184, 1314, 1494, 1775
MaeIII	GTNAC	6 cut(s)	231, 554, 1645, 1967, 2389, 2482
MbiI	CCGCTC	3 cut(s)	94, 778, 1769
MflI	RGATCY	2 cut(s)	878, 2719
MhlI	GDGCHC	3 cut(s)	52, 1114, 1452
MlsI	TGGCCA	2 cut(s)	62, 75
MluCI	AATT	8 cut(s)	618, 843, 1044, 1275, 1608, 1680, 1896, 1941
MluNI	TGGCCA	2 cut(s)	62, 75
Mly113I	GGCGCC	1 cut(s)	861
MlyI	GAGTC	2 cut(s)	1147, 1221
MmeI	TCCRAC	6 cut(s)	1240, 1576, 1610, 1844, 2385, 2607
Mox20I	TGGCCA	2 cut(s)	62, 75
MroI	TCCGGA	1 cut(s)	1036
MroNI	GCCGGC	1 cut(s)	392
MroXI	GAANNNNTTC	2 cut(s)	432, 2412
MscI	TGGCCA	2 cut(s)	62, 75
MseI	TTAA	5 cut(s)	782, 1431, 1679, 1757, 1940
MslI	CAYNNNNRTG	3 cut(s)	275, 1089, 2381
Msp20I	TGGCCA	2 cut(s)	62, 75
MspA1I	CMGCKG	1 cut(s)	1270
MspCI	CTTAAG	1 cut(s)	1430
MspR9I	CCNGG	9 cut(s)	59, 529, 542, 630, 748, 1727, 1956, 2084, 2617
MvaI	CCWGG	4 cut(s)	59, 1727, 1956, 2084
MvnI	CGCG	6 cut(s)	80, 917, 919, 1936, 2220, 2497
NaeI	GCCGGC	1 cut(s)	394
NarI	GGCGCC	1 cut(s)	861
NciI	CCSGG	5 cut(s)	529, 542, 630, 748, 2617
NcoI	CCATGG	2 cut(s)	63, 882
NgoMIV	GCCGGC	1 cut(s)	392
NheI	GCTAGC	1 cut(s)	1313
NlaIII	CATG	8 cut(s)	56, 67, 886, 1091, 1289, 1906, 2152, 2731
NlaIV	GGNNCC	9 cut(s)	512, 751, 862, 880, 1004, 1026, 1801, 1818, 2446
NmeAIII	GCCGAG	3 cut(s)	1771, 2392, 2688
NmuCI	GTSAC	6 cut(s)	231, 554, 1645, 1967, 2389, 2482
NruI	TCGCGA	1 cut(s)	2220
NspI	RCATGY	1 cut(s)	1906
NspV	TTCGAA	1 cut(s)	1423
PagI	TCATGA	1 cut(s)	2148
PasI	CCCWGGG	1 cut(s)	1955
PceI	AGGCCT	1 cut(s)	2011
PciI	ACATGT	1 cut(s)	1902
PciSI	GCTCTTC	1 cut(s)	1440
PcsI	WCGNNNNNNNCGW	4 cut(s)	1248, 1836, 2283, 2292
PdiI	GCCGGC	1 cut(s)	394
PdmI	GAANNNNTTC	2 cut(s)	432, 2412
PfeI	GAWTC	8 cut(s)	4, 343, 362, 583, 826, 1099, 1882, 2269
PfoI	TCCNGGA	1 cut(s)	2082
PleI	GAGTC	2 cut(s)	1146, 1220
PluTI	GGCGCC	1 cut(s)	864
PpsI	GAGTC	2 cut(s)	1146, 1220
PpuMI	RGGWCCY	1 cut(s)	511
PscI	ACATGT	1 cut(s)	1902
Psp124BI	GAGCTC	3 cut(s)	52, 1114, 1452
Psp5II	RGGWCCY	1 cut(s)	511
Psp6I	CCWGG	4 cut(s)	57, 1725, 1954, 2082
PspFI	CCCAGC	2 cut(s)	253, 1654
PspGI	CCWGG	4 cut(s)	57, 1725, 1954, 2082
PspN4I	GGNNCC	9 cut(s)	512, 751, 862, 880, 1004, 1026, 1801, 1818, 2446
PspPI	GGNCC	7 cut(s)	511, 750, 1598, 1632, 1657, 1817, 2660
PspPPI	RGGWCCY	1 cut(s)	511
PstNI	CAGNNNCTG	2 cut(s)	1378, 1592
PsuI	RGATCY	2 cut(s)	878, 2719
PvuII	CAGCTG	1 cut(s)	1270
RruI	TCGCGA	1 cut(s)	2220
RsaI	GTAC	2 cut(s)	962, 1564
RsaNI	GTAC	2 cut(s)	961, 1563
RseI	CAYNNNNRTG	3 cut(s)	275, 1089, 2381
SacI	GAGCTC	3 cut(s)	52, 1114, 1452
SapI	GCTCTTC	1 cut(s)	1440
SaqAI	TTAA	5 cut(s)	782, 1431, 1679, 1757, 1940
Sau96I	GGNCC	7 cut(s)	511, 750, 1598, 1632, 1657, 1817, 2660
SchI	GAGTC	2 cut(s)	1147, 1221
ScrFI	CCNGG	9 cut(s)	59, 529, 542, 630, 748, 1727, 1956, 2084, 2617
SduI	GDGCHC	3 cut(s)	52, 1114, 1452
SfoI	GGCGCC	1 cut(s)	862
SfuI	TTCGAA	1 cut(s)	1423
SinI	GGWCC	4 cut(s)	511, 1598, 1632, 1817
SmiMI	CAYNNNNRTG	3 cut(s)	275, 1089, 2381
SmlI	CTYRAG	4 cut(s)	356, 953, 1292, 1430
SmoI	CTYRAG	4 cut(s)	356, 953, 1292, 1430
Sse9I	AATT	8 cut(s)	618, 843, 1044, 1275, 1608, 1680, 1896, 1941
SseBI	AGGCCT	1 cut(s)	2011
SspDI	GGCGCC	1 cut(s)	860
SspMI	CTAG	8 cut(s)	258, 693, 857, 1029, 1184, 1314, 1494, 1775
SstI	GAGCTC	3 cut(s)	52, 1114, 1452
StuI	AGGCCT	1 cut(s)	2011
StyD4I	CCNGG	9 cut(s)	57, 527, 540, 628, 746, 1725, 1954, 2082, 2615
StyI	CCWWGG	2 cut(s)	63, 882
TaaI	ACNGT	6 cut(s)	1594, 2018, 2122, 2140, 2444, 2536
TaqII	GACCGA	1 cut(s)	2117
TasI	AATT	8 cut(s)	618, 843, 1044, 1275, 1608, 1680, 1896, 1941
TatI	WGTACW	1 cut(s)	960
TauI	GCSGC	3 cut(s)	94, 989, 2612
TfiI	GAWTC	8 cut(s)	4, 343, 362, 583, 826, 1099, 1882, 2269
Tru1I	TTAA	5 cut(s)	782, 1431, 1679, 1757, 1940
Tru9I	TTAA	5 cut(s)	782, 1431, 1679, 1757, 1940
TscAI	CASTG	4 cut(s)	133, 172, 1383, 2023
TseFI	GTSAC	6 cut(s)	231, 554, 1645, 1967, 2389, 2482
TseI	GCWGC	8 cut(s)	890, 1124, 1267, 1499, 2263, 2354, 2399, 2549
Tsp45I	GTSAC	6 cut(s)	231, 554, 1645, 1967, 2389, 2482
TspGWI	ACGGA	4 cut(s)	887, 2006, 2501, 2607
TspRI	CASTG	4 cut(s)	133, 172, 1383, 2023
Vha464I	CTTAAG	1 cut(s)	1430
VpaK11BI	GGWCC	4 cut(s)	511, 1598, 1632, 1817
XapI	RAATTY	1 cut(s)	843
XceI	RCATGY	1 cut(s)	1906
XmnI	GAANNNNTTC	2 cut(s)	432, 2412
XspI	CTAG	8 cut(s)	258, 693, 857, 1029, 1184, 1314, 1494, 1775

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.