Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0002819

Physical Location & Seq

Genomic Coordinates Reverse (-)

63431784 .. 63437120

Gene Structure 5337 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00270960.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2829 bp

ATGAATCCTGACAAATTTACTCACAAGACAAATGAGTCTCTGGCCGCGGCTCATGAACTGGCATCGGATGCTGGGCATGTGCAGTTCACGCCGCTCCATCTTGCAACCGCATTGGTTTCGGACCCTGCCGGCATTTTTCGACAGGCGATTGCCAATGCTAGTGGCAGTGAAGAGGGTCCGAAGTCGGTGGAGAGAGTTTTCAATCAGGCATTGAAGAAGCTCCCGTCTCAGTCCCCTCCCCCGGATCAAGTACCGGCCAGCACTACTCTCATTAAGGCAATTCGGAGAGCGCAGTCGGCTCAGAAAGATCGTGGGGATACACATTTGGCTGTTGATCAGTTGATTCTTGGCCTACTGGAGGATTCGCAGATTGGGGACTTGCTGAAAGAAGCTGGCATTGCTACAGCGAAGGTGAAATCGGAAGTTCAAAAGCTTCGTGGTAAAGAGGGGAAGAAGGTGGAGAGTGCCTCTGGGGACACGAATTTCCAAGCTTTGAATACTTATGGGAGAGACCTTGTTGAGCAGGCCGGGAAGCTTGACCCAGTGATTGGTAGAGATGAGGAGATAAGGAGGGTGGTTCGGATTCTCTCAAGGAGAACGAAGAATAACCCGGTGCTAATTGGAGAGCCAGGAGTTGGAAAAACAGCAGTAGTTGAAGGTTTGGCTCAGAGGATAGTAAGAGGGGATGTCCCTAGTAATCTGGCAGATGTGAGGCTTATTGCATTGGACATGGGTGCTCTAGTAGCCGGTGCCAAGTATAGAGGAGAATTTGAGGAGAGGTTGAAGGCTGTCTTGAAAGAAGTAGAAGAGGCCGAAGGGAAGGTGATTCTGTTTATTGATGAAATCCACCTTGTGCTTGGTGCTGGTAGAACAGAGGGGTCTATGGATGCCGCCAACCTTTTCAAGCCGATGCTCGCTAGAGGTCAACTCCGGTGCATTGGGGCAACGACTCTTGAAGAGTACAGGAAATATGTGGAGAAGGATGCTGCATTTGAGCGAAGGTTTCAACAAGTTTATGTGGCGGAACCTAGTGTTCCAGACACTGTTAGCATCCTTCGAGGCTTGAAGGAGAGATATGAAGGGCATCATGGTGTTAGAATTTTGGATAGAGCTTTAGTTGTCGCTGCACAACTATCGAGTCGATACATCACAGGGCGCCATCTCCCTGACAAGGCAATTGATCTAGTTGATGAAGCTTGTGCAAATGTGAGAGTCCAGCTTGACAGTCAACCAGAAGAAATTGACAACCTTGAGAGAAAGAGGATGCAGCTGGAAGTTGAGCTTCATGCGCTTGAAAAAGAAAAGGATAAAGCTAGCAAAGCTCGTCTTGTTGAAGTTCGGAGGGAGCTTGATGACTTAAGAGACAAGCTTCAGCCCTTGATGATGAAGTATAGAAAAGAGAAGGAAAGGATTGATGAGATTCGAAGGCTTAAGCAAAAGCGTGAAGAGCTCTTATTTGCTTTGACAGAGGCTGAAAGAAGATATGATCTAGCCAGGGCGGCTGATTTGCGTTATGGGGCTATTCAGGAAGTGGAGTCTGCTATTGCCCAGCTTGAAGGAAGCACAGAAGAGAACTTGATGTTGACTGAGACTGTTGGACCAGAACATATTGCAGAGGTTGTAAGCCGTTGGACCGGCATACCAGTAACAAGGCTCGGCCAGGATGACAAAGAGAGGTTAATTGGACTTGGTGAGAGATTGCATAAGAGAGTAGTGGGTCAAAACCAGGCAGTTGATGCTGTTGCAGAAGCCGTATTGAGATCAAGAGCTGGGTTAGGCAGACCACAACAACCAACTGGATCATTCCTTTTCTTGGGTCCAACTGGTGTTGGTAAAACTGAGCTTGCAAAGGCTCTTGCTGAACAACTTTTTGATGACGAAAATCTAATTGTTCGAATTGATATGTCTGAGTATATGGAGCAACATTCTGTTGCACGTCTGATTGGTGCTCCTCCTGGATATGTTGGTCATGAAGAAGGTGGACAACTCACAGAGGCAGTGAGGCGGAGGCCTTATAGTGTTATACTGTTTGATGAAGTGGAGAAAGCACACCCTGCAGTTTTCAATACTCTCCTGCAAGTTTTAGATGATGGCAGGTTAACTGATGGTCAAGGTCGTACAGTTGATTTTAGGAATACAGTGATCATTATGACTTCAAACCTTGGGGCGGAGCATCTCCTAACTGGTTTGTTGGGCAAATGTTCAATGCAAGTTGCTCGTGATCGAGTTATGCAAGAGGTATTGGTCCGAGATCAAGTGCTTACAATATTTAGAGATTCTCTTTTCCTTTTTCTAATGCAGAGTTTATCTGACATTTTACTGGAACAGGTGAGAACACACTTTAGGCCTGAATTGCTAAATCGGCTAGATGAGGTAGTTGTTTTCGATCCTCTTTCACGTGACCAACTGAGGAAGGTGGCTAGGTTACAAATGAAGGACGTTGCTGTCCGGCTTGCTGAGAGGGGTGTTGCTTTGGCGGTGACTGATAAAGCATTAGATTATATTCTGGACGAGAGTTATGATCCGGTTTATGGTGCTCGACCTATAAGAAGATGGCTTGAGAAGAGAGTGGTGACAGAGCTATCAAGAATGCTGATTAGAGAAGAGATAGATGAGAACTCGACTGTGTACATAGATGCAGGACCTATGACAAATGAGTTGGTTTACCGGGTTGAGAAGAATGGAGGGCTTGTTAATGCTGTCACCGGTCAGAAGTCAGATGTGTTGATACAACTTCCGAATGGACCAAGGCCGACTGATGATGCTCATCAGGCAGTTAAGAAGATGAAGATCGAGGAAATGGATGAGGACCTCGACATGGATGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006417 GO:0006950 GO:0006979 GO:0008150 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009636 GO:0009642 GO:0009644 GO:0009889 GO:0009891 GO:0009893 GO:0009941 GO:0009987 GO:0010035 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031967 GO:0031975 GO:0032268 GO:0032270 GO:0033554 GO:0034248 GO:0034250 GO:0034605 GO:0042221 GO:0042493 GO:0042542 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043335 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045727 GO:0046677 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051716 GO:0060255 GO:0065007 GO:0080090 GO:1901700 GO:2000112

KEGG Pathways

Metabolic & Signaling

ko04213 map04213

Pfam Domains

Protein Families

AAA AAA_2 ClpB_D2-small Clp_N

Protein Analysis

942

Amino Acids

105.23

Weight (kDa)

5.7

Isoelectric Point (pI)

36.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Clp_N	PF02861	5 - 130	6.1e-28	Clp repeat (R) N-terminal domain
AAA_16	PF13191	182 - 233	9.2e-07	AAA ATPase domain
NBD_SMAX1	PF23569	186 - 288	2.3e-09	SMAX1 nucleotide binding domain
AAA	PF00004	205 - 318	4e-13	ATPase family associated with various cellular activities (AAA)
AAA_lid_9	PF17871	344 - 444	3.2e-34	AAA lid domain
AAA_2	PF07724	598 - 792	4.2e-64	AAA domain (Cdc48 subfamily)
AAA_22	PF13401	600 - 697	1.9e-06	AAA domain
Sigma54_activat	PF00158	601 - 718	8.5e-06	Sigma-54 interaction domain
AAA_5	PF07728	602 - 721	1.7e-12	AAA domain (dynein-related subfamily)
AAA	PF00004	603 - 738	1e-10	ATPase family associated with various cellular activities (AAA)
ClpB_D2-small	PF10431	798 - 877	6.9e-23	C-terminal, D2-small domain, of ClpB protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0002819)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74310 AT1G74310 AT4G14670
fragaria_vesca	FvH4_5g14020 FvH4_5g14020 FvH4_5g14020 FvH4_5g14020 FvH4_7g22310 FvH4_7g22310 FvH4_7g22310
malus_domestica	MD06G1201600.v1.1 MD14G1210600.v1.1 MD14G1211000.v1.1
prunus_persica	Prupe.5G203700_v2.0.a1
pyrus_communis	pycom06g17750 pycom14g17400
rosa_chinensis	RchiOBHm_Chr1g0366791 RchiOBHm_Chr7g0182871 RchiOBHm_Chr7g0182901
rosa_laevigata	RLG00000005096 RLG00000005098 RLG00000027319
rosa_multiflora	Rmu_sc0000014.1_g000026 Rmu_sc0000318.1_g000026
rosa_roxburghii	Rroxscaffold_3G00230950 Rroxscaffold_3G00270960
rosa_rugosa	Rorug01G0331900 Rorug01G0333200 Rorug06G0451000
rosa_samantha	Rh1AG340000 Rh7AG054200 Rh7AG403300 Rh7BG053700 Rh7CG055300 Rh7DG053500 Rh7DG053600
rosa_wichuraiana	Rw0G008600 Rw1G030200 Rw7G004420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	34, 2444
Acc36I	ACCTGC	1 cut(s)	2085
AccB1I	GGYRCC	2 cut(s)	749, 1155
AccB7I	CCANNNNNTGG	2 cut(s)	548, 635
AccBSI	CCGCTC	1 cut(s)	94
AccII	CGCG	1 cut(s)	47
AclWI	GGATC	4 cut(s)	252, 1807, 2381, 2516
AcoI	YGGCCR	3 cut(s)	42, 255, 1657
AcsI	RAATTY	4 cut(s)	14, 481, 767, 1098
AcuI	CTGAAG	1 cut(s)	1355
AcvI	CACGTG	1 cut(s)	2399
AcyI	GRCGYC	1 cut(s)	1156
AdeI	CACNNNGTG	1 cut(s)	853
AfaI	GTAC	4 cut(s)	252, 962, 2119, 2630
AfiI	CCNNNNNNNGG	9 cut(s)	241, 358, 548, 635, 1171, 1813, 2167, 2531, 2818
AflII	CTTAAG	2 cut(s)	1357, 1430
AgeI	ACCGGT	1 cut(s)	2705
AjiI	CACGTC	1 cut(s)	1937
AjnI	CCWGG	5 cut(s)	628, 1493, 1659, 1725, 1954
Alw21I	GWGCWC	4 cut(s)	739, 1452, 1951, 2539
Alw26I	GTCTC	5 cut(s)	42, 231, 504, 1356, 1583
AlwI	GGATC	4 cut(s)	252, 1807, 2381, 2516
AlwNI	CAGNNNCTG	2 cut(s)	1043, 1472
AoxI	GGCC	9 cut(s)	42, 255, 349, 525, 810, 1657, 2009, 2345, 2750
ApeKI	GCWGC	3 cut(s)	986, 1124, 1267
ApoI	RAATTY	4 cut(s)	14, 481, 767, 1098
AsiGI	ACCGGT	1 cut(s)	2705
AspLEI	GCGC	3 cut(s)	292, 1158, 1291
AspS9I	GGNCC	9 cut(s)	121, 176, 1598, 1632, 1817, 2245, 2642, 2744, 2809
AsuC2I	CCSGG	4 cut(s)	242, 529, 611, 2669
AsuHPI	GGTGA	7 cut(s)	424, 835, 1703, 2341, 2491, 2584, 2695
AsuII	TTCGAA	2 cut(s)	1423, 1894
AsuNHI	GCTAGC	1 cut(s)	1313
AvaII	GGWCC	9 cut(s)	121, 176, 1598, 1632, 1817, 2245, 2642, 2744, 2809
BanI	GGYRCC	2 cut(s)	749, 1155
BanII	GRGCYC	1 cut(s)	1452
BauI	CACGAG	1 cut(s)	2217
BbrPI	CACGTG	1 cut(s)	2399
Bbv12I	GWGCWC	4 cut(s)	739, 1452, 1951, 2539
BbvI	GCAGC	3 cut(s)	973, 1111, 1279
BccI	CCATC	5 cut(s)	105, 1167, 2084, 2099, 2547
BceAI	ACGGC	2 cut(s)	1611, 1736
BcgI	CGANNNNNNTGC	6 cut(s)	99, 133, 1116, 1150, 2198, 2232
BciT130I	CCWGG	5 cut(s)	630, 1495, 1661, 1727, 1956
BciVI	GTATCC	1 cut(s)	310
BclI	TGATCA	2 cut(s)	334, 2142
BcnI	CCSGG	4 cut(s)	242, 529, 611, 2669
BcoDI	GTCTC	5 cut(s)	42, 231, 504, 1356, 1583
BfmI	CTRYAG	2 cut(s)	402, 2055
BfoI	RGCGCY	1 cut(s)	1159
BfrI	CTTAAG	2 cut(s)	1357, 1430
BfuAI	ACCTGC	1 cut(s)	2085
BfuI	GTATCC	1 cut(s)	310
BglI	GCCNNNNNGGC	1 cut(s)	1499
BisI	GCNGC	8 cut(s)	45, 48, 92, 891, 987, 1125, 1268, 1500
BlsI	GCNGC	8 cut(s)	46, 49, 93, 892, 988, 1126, 1269, 1501
Bme1390I	CCNGG	9 cut(s)	242, 529, 611, 630, 1495, 1661, 1727, 1956, 2669
Bme18I	GGWCC	9 cut(s)	121, 176, 1598, 1632, 1817, 2245, 2642, 2744, 2809
BmgBI	CACGTC	1 cut(s)	1937
BmgT120I	GGNCC	9 cut(s)	121, 176, 1598, 1632, 1817, 2245, 2642, 2744, 2809
BmiI	GGNNCC	6 cut(s)	123, 177, 751, 1026, 1157, 1818
BmrFI	CCNGG	9 cut(s)	242, 529, 611, 630, 1495, 1661, 1727, 1956, 2669
BmrI	ACTGGG	1 cut(s)	536
BmtI	GCTAGC	1 cut(s)	1317
BmuI	ACTGGG	1 cut(s)	536
BplI	GAGNNNNNCTC	4 cut(s)	452, 484, 912, 944
BpmI	CTGGAG	1 cut(s)	377
Bpu14I	TTCGAA	2 cut(s)	1423, 1894
BpuEI	CTTGAG	3 cut(s)	574, 1271, 2579
BpuMI	CCSGG	4 cut(s)	242, 529, 611, 2669
BsaAI	YACGTR	1 cut(s)	2399
BsaBI	GATNNNNATC	2 cut(s)	2766, 2789
BsaHI	GRCGYC	1 cut(s)	1156
BsaI	GGTCTC	1 cut(s)	504
BsaJI	CCNNGG	5 cut(s)	45, 240, 1494, 2161, 2747
BsaWI	WCCGGW	3 cut(s)	930, 2524, 2705
BsaXI	ACNNNNNCTCC	2 cut(s)	2676, 2706
Bsc4I	CCNNNNNNNGG	9 cut(s)	241, 358, 548, 635, 1171, 1813, 2167, 2531, 2818
Bse118I	RCCGGY	5 cut(s)	128, 253, 746, 1634, 2705
Bse1I	ACTGG	8 cut(s)	63, 360, 542, 1643, 1801, 1828, 2188, 2325
Bse3DI	GCAATG	1 cut(s)	396
Bse8I	GATNNNNATC	2 cut(s)	2766, 2789
BseBI	CCWGG	5 cut(s)	630, 1495, 1661, 1727, 1956
BseDI	CCNNGG	5 cut(s)	45, 240, 1494, 2161, 2747
BseGI	GGATG	9 cut(s)	73, 691, 892, 988, 1050, 1269, 1669, 2809, 2827
BseJI	GATNNNNATC	2 cut(s)	2766, 2789
BseLI	CCNNNNNNNGG	9 cut(s)	241, 358, 548, 635, 1171, 1813, 2167, 2531, 2818
BseMI	GCAATG	1 cut(s)	396
BseMII	CTCAG	8 cut(s)	242, 314, 680, 1578, 1830, 1899, 2399, 2448
BseNI	ACTGG	8 cut(s)	63, 360, 542, 1643, 1801, 1828, 2188, 2325
BseRI	GAGGAG	4 cut(s)	575, 777, 788, 1941
BseXI	GCAGC	3 cut(s)	973, 1111, 1279
BseYI	CCCAGC	3 cut(s)	71, 1548, 1769
BsgI	GTGCAG	2 cut(s)	101, 1110
Bsh1236I	CGCG	1 cut(s)	47
BshFI	GGCC	9 cut(s)	44, 257, 351, 527, 812, 1659, 2011, 2347, 2752
BshNI	GGYRCC	2 cut(s)	749, 1155
BshTI	ACCGGT	1 cut(s)	2705
BsiHKAI	GWGCWC	4 cut(s)	739, 1452, 1951, 2539
BslFI	GGGAC	4 cut(s)	217, 389, 488, 674
BslI	CCNNNNNNNGG	9 cut(s)	241, 358, 548, 635, 1171, 1813, 2167, 2531, 2818
BsmAI	GTCTC	5 cut(s)	42, 231, 504, 1356, 1583
BsmBI	CGTCTC	1 cut(s)	231
BsmFI	GGGAC	4 cut(s)	217, 389, 488, 674
BsmI	GAATGC	1 cut(s)	2595
BsnI	GGCC	9 cut(s)	44, 257, 351, 527, 812, 1659, 2011, 2347, 2752
Bso31I	GGTCTC	1 cut(s)	504
Bsp119I	TTCGAA	2 cut(s)	1423, 1894
Bsp1286I	GDGCHC	4 cut(s)	739, 1452, 1951, 2539
Bsp1407I	TGTACA	1 cut(s)	2628
BspANI	GGCC	9 cut(s)	44, 257, 351, 527, 812, 1659, 2011, 2347, 2752
BspCNI	CTCAG	8 cut(s)	241, 313, 679, 1579, 1831, 1900, 2400, 2449
BspFNI	CGCG	1 cut(s)	47
BspHI	TCATGA	2 cut(s)	52, 1969
BspLI	GGNNCC	6 cut(s)	123, 177, 751, 1026, 1157, 1818
BspMAI	CTGCAG	1 cut(s)	2059
BspMI	ACCTGC	1 cut(s)	2085
BspOI	GCTAGC	1 cut(s)	1317
BspPI	GGATC	4 cut(s)	252, 1807, 2381, 2516
BspQI	GCTCTTC	1 cut(s)	1440
BspT104I	TTCGAA	2 cut(s)	1423, 1894
BspT107I	GGYRCC	2 cut(s)	749, 1155
BspTI	CTTAAG	2 cut(s)	1357, 1430
BspTNI	GGTCTC	1 cut(s)	504
BsrBI	CCGCTC	1 cut(s)	94
BsrDI	GCAATG	1 cut(s)	396
BsrFI	RCCGGY	5 cut(s)	128, 253, 746, 1634, 2705
BsrGI	TGTACA	1 cut(s)	2628
BsrI	ACTGG	8 cut(s)	63, 360, 542, 1643, 1801, 1828, 2188, 2325
BssAI	RCCGGY	5 cut(s)	128, 253, 746, 1634, 2705
BssECI	CCNNGG	5 cut(s)	45, 240, 1494, 2161, 2747
BssNI	GRCGYC	1 cut(s)	1156
BssSI	CACGAG	1 cut(s)	2217
BssT1I	CCWWGG	2 cut(s)	2161, 2747
Bst2BI	CACGAG	1 cut(s)	2217
Bst2UI	CCWGG	5 cut(s)	630, 1495, 1661, 1727, 1956
Bst4CI	ACNGT	7 cut(s)	1045, 1226, 1594, 2028, 2122, 2140, 2626
Bst6I	CTCTTC	7 cut(s)	165, 801, 951, 1440, 1563, 2558, 2598
BstACI	GRCGYC	1 cut(s)	1156
BstAFI	CTTAAG	2 cut(s)	1357, 1430
BstAPI	GCANNNNNTGC	3 cut(s)	68, 1736, 2054
BstAUI	TGTACA	1 cut(s)	2628
BstBAI	YACGTR	1 cut(s)	2399
BstBI	TTCGAA	2 cut(s)	1423, 1894
BstC8I	GCNNGC	8 cut(s)	130, 259, 394, 525, 915, 1315, 1845, 2454
BstDEI	CTNAG	8 cut(s)	228, 300, 666, 1587, 1839, 1908, 2408, 2457
BstDSI	CCRYGG	1 cut(s)	45
BstENI	CCTNNNNNAGG	1 cut(s)	356
BstF5I	GGATG	9 cut(s)	73, 691, 892, 988, 1050, 1269, 1669, 2809, 2827
BstFNI	CGCG	1 cut(s)	47
BstH2I	RGCGCY	1 cut(s)	1159
BstHHI	GCGC	3 cut(s)	292, 1158, 1291
BstMAI	GTCTC	5 cut(s)	42, 231, 504, 1356, 1583
BstNI	CCWGG	5 cut(s)	630, 1495, 1661, 1727, 1956
BstNSI	RCATGY	1 cut(s)	80
BstSCI	CCNGG	9 cut(s)	240, 527, 609, 628, 1493, 1659, 1725, 1954, 2667
BstSFI	CTRYAG	2 cut(s)	402, 2055
BstUI	CGCG	1 cut(s)	47
BstV1I	GCAGC	3 cut(s)	973, 1111, 1279
BsuI	GTATCC	1 cut(s)	310
BsuRI	GGCC	9 cut(s)	44, 257, 351, 527, 812, 1659, 2011, 2347, 2752
BtgI	CCRYGG	1 cut(s)	45
BtrI	CACGTC	1 cut(s)	1937
BtsCI	GGATG	9 cut(s)	73, 691, 892, 988, 1050, 1269, 1669, 2809, 2827
BtsI	GCAGTG	2 cut(s)	172, 2004
BtsIMutI	CAGTG	5 cut(s)	172, 549, 1041, 2004, 2145
BveI	ACCTGC	1 cut(s)	2085
Cac8I	GCNNGC	8 cut(s)	130, 259, 394, 525, 915, 1315, 1845, 2454
CaiI	CAGNNNCTG	2 cut(s)	1043, 1472
CciI	TCATGA	2 cut(s)	52, 1969
CfoI	GCGC	3 cut(s)	292, 1158, 1291
Cfr10I	RCCGGY	5 cut(s)	128, 253, 746, 1634, 2705
Cfr13I	GGNCC	9 cut(s)	121, 176, 1598, 1632, 1817, 2245, 2642, 2744, 2809
Cfr42I	CCGCGG	1 cut(s)	48
Csp6I	GTAC	4 cut(s)	251, 961, 2118, 2629
CspAI	ACCGGT	1 cut(s)	2705
CspCI	CAANNNNNGTGG	2 cut(s)	142, 177
CviAII	CATG	7 cut(s)	53, 77, 730, 1088, 1286, 1970, 2818
CviQI	GTAC	4 cut(s)	251, 961, 2118, 2629
DdeI	CTNAG	8 cut(s)	228, 300, 666, 1587, 1839, 1908, 2408, 2457
DinI	GGCGCC	1 cut(s)	1157
DraIII	CACNNNGTG	1 cut(s)	853
DrdI	GACNNNNNNGTC	2 cut(s)	34, 2444
DseDI	GACNNNNNNGTC	2 cut(s)	34, 2444
EaeI	YGGCCR	3 cut(s)	42, 255, 1657
Eam1104I	CTCTTC	7 cut(s)	165, 801, 951, 1440, 1563, 2558, 2598
EarI	CTCTTC	7 cut(s)	165, 801, 951, 1440, 1563, 2558, 2598
EciI	GGCGGA	3 cut(s)	1037, 2020, 2183
Ecl136II	GAGCTC	1 cut(s)	1450
Eco130I	CCWWGG	2 cut(s)	2161, 2747
Eco147I	AGGCCT	2 cut(s)	2011, 2347
Eco24I	GRGCYC	1 cut(s)	1452
Eco31I	GGTCTC	1 cut(s)	504
Eco47I	GGWCC	9 cut(s)	121, 176, 1598, 1632, 1817, 2245, 2642, 2744, 2809
Eco53kI	GAGCTC	1 cut(s)	1450
Eco57I	CTGAAG	1 cut(s)	1355
Eco72I	CACGTG	1 cut(s)	2399
EcoICRI	GAGCTC	1 cut(s)	1450
EcoNI	CCTNNNNNAGG	1 cut(s)	356
EcoO109I	RGGNCCY	2 cut(s)	2642, 2809
EcoRII	CCWGG	5 cut(s)	628, 1493, 1659, 1725, 1954
EcoT14I	CCWWGG	2 cut(s)	2161, 2747
EcoT38I	GRGCYC	1 cut(s)	1452
EgeI	GGCGCC	1 cut(s)	1157
EheI	GGCGCC	1 cut(s)	1157
ErhI	CCWWGG	2 cut(s)	2161, 2747
Esp3I	CGTCTC	1 cut(s)	231
FaeI	CATG	7 cut(s)	56, 80, 733, 1091, 1289, 1973, 2821
FalI	AAGNNNNNCTT	6 cut(s)	776, 808, 1266, 1298, 1311, 1343
FaqI	GGGAC	4 cut(s)	217, 389, 488, 674
FatI	CATG	7 cut(s)	52, 76, 729, 1087, 1285, 1969, 2817
FbaI	TGATCA	2 cut(s)	334, 2142
Fnu4HI	GCNGC	8 cut(s)	45, 48, 92, 891, 987, 1125, 1268, 1500
FokI	GGATG	8 cut(s)	80, 698, 899, 995, 1037, 1276, 1676, 2816
FriOI	GRGCYC	1 cut(s)	1452
Fsp4HI	GCNGC	8 cut(s)	45, 48, 92, 891, 987, 1125, 1268, 1500
GlaI	GCGC	3 cut(s)	291, 1157, 1290
GluI	GCNGC	8 cut(s)	45, 48, 92, 891, 987, 1125, 1268, 1500
GsaI	CCCAGC	3 cut(s)	75, 1552, 1773
GsuI	CTGGAG	1 cut(s)	377
HaeII	RGCGCY	1 cut(s)	1159
HaeIII	GGCC	9 cut(s)	44, 257, 351, 527, 812, 1659, 2011, 2347, 2752
HhaI	GCGC	3 cut(s)	292, 1158, 1291
Hin1I	GRCGYC	1 cut(s)	1156
Hin1II	CATG	7 cut(s)	56, 80, 733, 1091, 1289, 1973, 2821
Hin6I	GCGC	3 cut(s)	290, 1156, 1289
HinP1I	GCGC	3 cut(s)	290, 1156, 1289
HincII	GTYRAC	4 cut(s)	926, 1229, 1584, 2100
HindII	GTYRAC	4 cut(s)	926, 1229, 1584, 2100
HindIII	AAGCTT	5 cut(s)	431, 489, 533, 1194, 1367
HpaI	GTTAAC	1 cut(s)	2100
HphI	GGTGA	7 cut(s)	424, 835, 1703, 2341, 2491, 2584, 2695
Hpy166II	GTNNAC	8 cut(s)	87, 926, 1229, 1584, 1982, 2100, 2629, 2665
Hpy8I	GTNNAC	8 cut(s)	87, 926, 1229, 1584, 1982, 2100, 2629, 2665
HpyCH4III	ACNGT	7 cut(s)	1045, 1226, 1594, 2028, 2122, 2140, 2626
HpyCH4IV	ACGT	3 cut(s)	1936, 2398, 2439
HpyF3I	CTNAG	8 cut(s)	228, 300, 666, 1587, 1839, 1908, 2408, 2457
HpySE526I	ACGT	3 cut(s)	1936, 2398, 2439
Hsp92I	GRCGYC	1 cut(s)	1156
Hsp92II	CATG	7 cut(s)	56, 80, 733, 1091, 1289, 1973, 2821
HspAI	GCGC	3 cut(s)	290, 1156, 1289
KasI	GGCGCC	1 cut(s)	1155
KroI	GCCGGC	1 cut(s)	128
KroNI	GCCGGC	1 cut(s)	130
Ksp22I	TGATCA	2 cut(s)	334, 2142
KspAI	GTTAAC	1 cut(s)	2100
KspI	CCGCGG	1 cut(s)	48
LguI	GCTCTTC	1 cut(s)	1440
LmnI	GCTCC	6 cut(s)	99, 225, 1345, 1918, 1954, 2170
Lsp1109I	GCAGC	3 cut(s)	973, 1111, 1279
MaeII	ACGT	3 cut(s)	1936, 2398, 2439
MaeIII	GTNAC	6 cut(s)	1645, 2399, 2424, 2479, 2572, 2701
MbiI	CCGCTC	1 cut(s)	94
MfeI	CAATTG	1 cut(s)	1176
MhlI	GDGCHC	4 cut(s)	739, 1452, 1951, 2539
Mly113I	GGCGCC	1 cut(s)	1156
MlyI	GAGTC	5 cut(s)	44, 943, 1147, 1221, 1544
MmeI	TCCRAC	4 cut(s)	616, 1576, 1610, 1844
MroNI	GCCGGC	1 cut(s)	128
MseI	TTAA	7 cut(s)	273, 1358, 1431, 1679, 2099, 2694, 2778
MslI	CAYNNNNRTG	1 cut(s)	1089
MspA1I	CMGCKG	2 cut(s)	47, 1270
MspCI	CTTAAG	2 cut(s)	1357, 1430
MspR9I	CCNGG	9 cut(s)	242, 529, 611, 630, 1495, 1661, 1727, 1956, 2669
MunI	CAATTG	1 cut(s)	1176
Mva1269I	GAATGC	1 cut(s)	2595
MvaI	CCWGG	5 cut(s)	630, 1495, 1661, 1727, 1956
MvnI	CGCG	1 cut(s)	47
NaeI	GCCGGC	1 cut(s)	130
NarI	GGCGCC	1 cut(s)	1156
NciI	CCSGG	4 cut(s)	242, 529, 611, 2669
NgoMIV	GCCGGC	1 cut(s)	128
NheI	GCTAGC	1 cut(s)	1313
NlaIII	CATG	7 cut(s)	56, 80, 733, 1091, 1289, 1973, 2821
NlaIV	GGNNCC	6 cut(s)	123, 177, 751, 1026, 1157, 1818
NmeAIII	GCCGAG	1 cut(s)	1635
NmuCI	GTSAC	4 cut(s)	2399, 2479, 2572, 2701
NspI	RCATGY	1 cut(s)	80
NspV	TTCGAA	2 cut(s)	1423, 1894
PagI	TCATGA	2 cut(s)	52, 1969
PceI	AGGCCT	2 cut(s)	2011, 2347
PciSI	GCTCTTC	1 cut(s)	1440
PctI	GAATGC	1 cut(s)	2595
PdiI	GCCGGC	1 cut(s)	130
PfeI	GAWTC	7 cut(s)	4, 343, 362, 583, 826, 1420, 2276
PflMI	CCANNNNNTGG	2 cut(s)	548, 635
PfoI	TCCNGGA	1 cut(s)	1954
PinAI	ACCGGT	1 cut(s)	2705
PkrI	GCNGC	8 cut(s)	46, 49, 93, 892, 988, 1126, 1269, 1501
PleI	GAGTC	5 cut(s)	43, 943, 1146, 1220, 1543
PluTI	GGCGCC	1 cut(s)	1159
PmaCI	CACGTG	1 cut(s)	2399
PmlI	CACGTG	1 cut(s)	2399
PpsI	GAGTC	5 cut(s)	43, 943, 1146, 1220, 1543
Ppu21I	YACGTR	1 cut(s)	2399
PpuMI	RGGWCCY	2 cut(s)	2642, 2809
Psp124BI	GAGCTC	1 cut(s)	1452
Psp5II	RGGWCCY	2 cut(s)	2642, 2809
Psp6I	CCWGG	5 cut(s)	628, 1493, 1659, 1725, 1954
PspCI	CACGTG	1 cut(s)	2399
PspFI	CCCAGC	3 cut(s)	71, 1548, 1769
PspGI	CCWGG	5 cut(s)	628, 1493, 1659, 1725, 1954
PspN4I	GGNNCC	6 cut(s)	123, 177, 751, 1026, 1157, 1818
PspPI	GGNCC	9 cut(s)	121, 176, 1598, 1632, 1817, 2245, 2642, 2744, 2809
PspPPI	RGGWCCY	2 cut(s)	2642, 2809
PstI	CTGCAG	1 cut(s)	2059
PstNI	CAGNNNCTG	2 cut(s)	1043, 1472
PvuII	CAGCTG	1 cut(s)	1270
RsaI	GTAC	4 cut(s)	252, 962, 2119, 2630
RsaNI	GTAC	4 cut(s)	251, 961, 2118, 2629
RseI	CAYNNNNRTG	1 cut(s)	1089
SacI	GAGCTC	1 cut(s)	1452
SacII	CCGCGG	1 cut(s)	48
SapI	GCTCTTC	1 cut(s)	1440
SaqAI	TTAA	7 cut(s)	273, 1358, 1431, 1679, 2099, 2694, 2778
SatI	GCNGC	8 cut(s)	45, 48, 92, 891, 987, 1125, 1268, 1500
Sau96I	GGNCC	9 cut(s)	121, 176, 1598, 1632, 1817, 2245, 2642, 2744, 2809
SchI	GAGTC	5 cut(s)	44, 943, 1147, 1221, 1544
ScrFI	CCNGG	9 cut(s)	242, 529, 611, 630, 1495, 1661, 1727, 1956, 2669
SduI	GDGCHC	4 cut(s)	739, 1452, 1951, 2539
SfcI	CTRYAG	2 cut(s)	402, 2055
SfoI	GGCGCC	1 cut(s)	1157
Sfr303I	CCGCGG	1 cut(s)	48
SfuI	TTCGAA	2 cut(s)	1423, 1894
SgrBI	CCGCGG	1 cut(s)	48
SinI	GGWCC	9 cut(s)	121, 176, 1598, 1632, 1817, 2245, 2642, 2744, 2809
SmiMI	CAYNNNNRTG	1 cut(s)	1089
SmlI	CTYRAG	5 cut(s)	589, 1250, 1357, 1430, 2558
SmoI	CTYRAG	5 cut(s)	589, 1250, 1357, 1430, 2558
SseBI	AGGCCT	2 cut(s)	2011, 2347
SspDI	GGCGCC	1 cut(s)	1155
SspI	AATATT	1 cut(s)	2268
SstI	GAGCTC	1 cut(s)	1452
StuI	AGGCCT	2 cut(s)	2011, 2347
StyD4I	CCNGG	9 cut(s)	240, 527, 609, 628, 1493, 1659, 1725, 1954, 2667
StyI	CCWWGG	2 cut(s)	2161, 2747
TaaI	ACNGT	7 cut(s)	1045, 1226, 1594, 2028, 2122, 2140, 2626
TaiI	ACGT	3 cut(s)	1939, 2401, 2442
TatI	WGTACW	2 cut(s)	960, 2628
TauI	GCSGC	5 cut(s)	47, 50, 94, 893, 1502
TfiI	GAWTC	7 cut(s)	4, 343, 362, 583, 826, 1420, 2276
Tru1I	TTAA	7 cut(s)	273, 1358, 1431, 1679, 2099, 2694, 2778
Tru9I	TTAA	7 cut(s)	273, 1358, 1431, 1679, 2099, 2694, 2778
TscAI	CASTG	5 cut(s)	172, 549, 1048, 2004, 2145
TseFI	GTSAC	4 cut(s)	2399, 2479, 2572, 2701
TseI	GCWGC	3 cut(s)	986, 1124, 1267
Tsp45I	GTSAC	4 cut(s)	2399, 2479, 2572, 2701
TspRI	CASTG	5 cut(s)	172, 549, 1048, 2004, 2145
Van91I	CCANNNNNTGG	2 cut(s)	548, 635
Vha464I	CTTAAG	2 cut(s)	1357, 1430
VpaK11BI	GGWCC	9 cut(s)	121, 176, 1598, 1632, 1817, 2245, 2642, 2744, 2809
XagI	CCTNNNNNAGG	1 cut(s)	356
XapI	RAATTY	4 cut(s)	14, 481, 767, 1098
XceI	RCATGY	1 cut(s)	80
XcmI	CCANNNNNNNNNTGG	1 cut(s)	854

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.