Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G76170 AT1G76170 AT1G76170 AT1G76170 AT1G76170 AT1G76170 AT1G76170 AT2G44270 |
| fragaria_vesca | FvH4_2g37771 FvH4_2g37771 FvH4_2g37771 |
| malus_domestica | MD08G1004800.v1.1 MD15G1004300.v1.1 |
| prunus_persica | Prupe.1G356900_v2.0.a1 |
| pyrus_communis | pycom08g00360 pycom08g00370 pycom15g00310 pycom15g00320 |
| rosa_chinensis | RchiOBHm_Chr6g0303951 RchiOBHm_Chr6g0303961 |
| rosa_laevigata | RLG00000011030 |
| rosa_multiflora | Rmu_sc0004644.1_g000013 Rmu_sc0004644.1_g000014 |
| rosa_roxburghii | Rroxscaffold_7G00164230 |
| rosa_rugosa | Rorug06G0326200 |
| rosa_samantha | Rh6AG440500 Rh6BG462400 Rh6CG452800 Rh6DG439800 |
| rosa_wichuraiana | Rw6G038150 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 930 |
| AatII | GACGTC | 1 cut(s) | 190 |
| AccB1I | GGYRCC | 1 cut(s) | 128 |
| AccI | GTMKAC | 1 cut(s) | 403 |
| AclWI | GGATC | 1 cut(s) | 991 |
| AcuI | CTGAAG | 3 cut(s) | 135, 287, 748 |
| AcyI | GRCGYC | 1 cut(s) | 187 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 406, 797, 890 |
| AgsI | TTSAA | 6 cut(s) | 287, 370, 436, 586, 628, 871 |
| AluBI | AGCT | 4 cut(s) | 425, 448, 634, 848 |
| AluI | AGCT | 4 cut(s) | 425, 448, 634, 848 |
| Alw21I | GWGCWC | 1 cut(s) | 536 |
| Alw26I | GTCTC | 1 cut(s) | 257 |
| Alw44I | GTGCAC | 1 cut(s) | 532 |
| AlwI | GGATC | 1 cut(s) | 991 |
| Ama87I | CYCGRG | 1 cut(s) | 260 |
| AoxI | GGCC | 1 cut(s) | 722 |
| ApaLI | GTGCAC | 1 cut(s) | 532 |
| ApeKI | GCWGC | 1 cut(s) | 425 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 474, 506 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 69, 145 |
| AspS9I | GGNCC | 1 cut(s) | 560 |
| AsuHPI | GGTGA | 4 cut(s) | 65, 122, 767, 935 |
| AvaI | CYCGRG | 1 cut(s) | 260 |
| AvaII | GGWCC | 1 cut(s) | 560 |
| BaeGI | GKGCMC | 1 cut(s) | 536 |
| BanI | GGYRCC | 1 cut(s) | 128 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 623, 655 |
| BbsI | GAAGAC | 2 cut(s) | 765, 907 |
| Bbv12I | GWGCWC | 1 cut(s) | 536 |
| BbvI | GCAGC | 1 cut(s) | 412 |
| BccI | CCATC | 3 cut(s) | 327, 551, 883 |
| BcoDI | GTCTC | 1 cut(s) | 257 |
| BfaI | CTAG | 4 cut(s) | 449, 519, 828, 945 |
| BfmI | CTRYAG | 3 cut(s) | 123, 546, 963 |
| BglII | AGATCT | 2 cut(s) | 322, 947 |
| BisI | GCNGC | 1 cut(s) | 426 |
| BlsI | GCNGC | 1 cut(s) | 427 |
| Bme18I | GGWCC | 1 cut(s) | 560 |
| BmeT110I | CYCGRG | 1 cut(s) | 260 |
| BmgT120I | GGNCC | 1 cut(s) | 560 |
| BmiI | GGNNCC | 1 cut(s) | 130 |
| BpiI | GAAGAC | 2 cut(s) | 765, 907 |
| Bpu10I | CCTNAGC | 1 cut(s) | 792 |
| BsaHI | GRCGYC | 1 cut(s) | 187 |
| BsaJI | CCNNGG | 2 cut(s) | 725, 874 |
| BsaWI | WCCGGW | 1 cut(s) | 134 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 629, 659 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 406, 797, 890 |
| Bse1I | ACTGG | 1 cut(s) | 460 |
| BseDI | CCNNGG | 2 cut(s) | 725, 874 |
| BseGI | GGATG | 1 cut(s) | 338 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 406, 797, 890 |
| BseMII | CTCAG | 1 cut(s) | 783 |
| BseNI | ACTGG | 1 cut(s) | 460 |
| BseRI | GAGGAG | 2 cut(s) | 51, 435 |
| BseSI | GKGCMC | 1 cut(s) | 536 |
| BseXI | GCAGC | 1 cut(s) | 412 |
| BshFI | GGCC | 1 cut(s) | 724 |
| BshNI | GGYRCC | 1 cut(s) | 128 |
| BsiHKAI | GWGCWC | 1 cut(s) | 536 |
| BsiHKCI | CYCGRG | 1 cut(s) | 260 |
| BsiSI | CCGG | 1 cut(s) | 135 |
| BslFI | GGGAC | 1 cut(s) | 814 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 406, 797, 890 |
| BsmAI | GTCTC | 1 cut(s) | 257 |
| BsmFI | GGGAC | 1 cut(s) | 814 |
| BsmI | GAATGC | 1 cut(s) | 659 |
| BsnI | GGCC | 1 cut(s) | 724 |
| BsoBI | CYCGRG | 1 cut(s) | 260 |
| Bsp1286I | GDGCHC | 1 cut(s) | 536 |
| Bsp143I | GATC | 6 cut(s) | 205, 322, 360, 415, 947, 983 |
| BspANI | GGCC | 1 cut(s) | 724 |
| BspCNI | CTCAG | 1 cut(s) | 784 |
| BspLI | GGNNCC | 1 cut(s) | 130 |
| BspPI | GGATC | 1 cut(s) | 991 |
| BspQI | GCTCTTC | 1 cut(s) | 14 |
| BspT107I | GGYRCC | 1 cut(s) | 128 |
| BsrI | ACTGG | 1 cut(s) | 460 |
| BssECI | CCNNGG | 2 cut(s) | 725, 874 |
| BssMI | GATC | 6 cut(s) | 205, 322, 360, 415, 947, 983 |
| BssNI | GRCGYC | 1 cut(s) | 187 |
| BssT1I | CCWWGG | 1 cut(s) | 725 |
| Bst4CI | ACNGT | 4 cut(s) | 194, 268, 487, 875 |
| Bst6I | CTCTTC | 4 cut(s) | 9, 14, 60, 306 |
| BstACI | GRCGYC | 1 cut(s) | 187 |
| BstC8I | GCNNGC | 1 cut(s) | 159 |
| BstDEI | CTNAG | 3 cut(s) | 719, 792, 885 |
| BstDSI | CCRYGG | 1 cut(s) | 874 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 795 |
| BstF5I | GGATG | 1 cut(s) | 338 |
| BstKTI | GATC | 6 cut(s) | 208, 325, 363, 418, 950, 986 |
| BstMAI | GTCTC | 1 cut(s) | 257 |
| BstMBI | GATC | 6 cut(s) | 205, 322, 360, 415, 947, 983 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 128 |
| BstSFI | CTRYAG | 3 cut(s) | 123, 546, 963 |
| BstSLI | GKGCMC | 1 cut(s) | 536 |
| BstV1I | GCAGC | 1 cut(s) | 412 |
| BstV2I | GAAGAC | 2 cut(s) | 765, 907 |
| BstX2I | RGATCY | 3 cut(s) | 322, 947, 983 |
| BstYI | RGATCY | 3 cut(s) | 322, 947, 983 |
| BsuRI | GGCC | 1 cut(s) | 724 |
| BtgI | CCRYGG | 1 cut(s) | 874 |
| BtgZI | GCGATG | 1 cut(s) | 826 |
| BtsCI | GGATG | 1 cut(s) | 338 |
| BtsIMutI | CAGTG | 3 cut(s) | 159, 453, 648 |
| Cac8I | GCNNGC | 1 cut(s) | 159 |
| Cfr13I | GGNCC | 1 cut(s) | 560 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 961, 996 |
| CviAII | CATG | 1 cut(s) | 613 |
| CviJI | RGCY | 9 cut(s) | 425, 448, 479, 634, 724, 731, 831, 848, 944 |
| CviKI_1 | RGCY | 9 cut(s) | 425, 448, 479, 634, 724, 731, 831, 848, 944 |
| DdeI | CTNAG | 3 cut(s) | 719, 792, 885 |
| DpnI | GATC | 6 cut(s) | 207, 324, 362, 417, 949, 985 |
| DpnII | GATC | 6 cut(s) | 205, 322, 360, 415, 947, 983 |
| Eam1104I | CTCTTC | 4 cut(s) | 9, 14, 60, 306 |
| EarI | CTCTTC | 4 cut(s) | 9, 14, 60, 306 |
| Eco130I | CCWWGG | 1 cut(s) | 725 |
| Eco32I | GATATC | 1 cut(s) | 742 |
| Eco47I | GGWCC | 1 cut(s) | 560 |
| Eco57I | CTGAAG | 3 cut(s) | 135, 287, 748 |
| Eco88I | CYCGRG | 1 cut(s) | 260 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 795 |
| EcoRV | GATATC | 1 cut(s) | 742 |
| EcoT14I | CCWWGG | 1 cut(s) | 725 |
| EcoT22I | ATGCAT | 1 cut(s) | 659 |
| ErhI | CCWWGG | 1 cut(s) | 725 |
| FaeI | CATG | 1 cut(s) | 616 |
| FaqI | GGGAC | 1 cut(s) | 814 |
| FatI | CATG | 1 cut(s) | 612 |
| FauNDI | CATATG | 1 cut(s) | 163 |
| FblI | GTMKAC | 1 cut(s) | 403 |
| Fnu4HI | GCNGC | 1 cut(s) | 426 |
| FokI | GGATG | 1 cut(s) | 345 |
| Fsp4HI | GCNGC | 1 cut(s) | 426 |
| FspBI | CTAG | 4 cut(s) | 449, 519, 828, 945 |
| GluI | GCNGC | 1 cut(s) | 426 |
| HaeIII | GGCC | 1 cut(s) | 724 |
| HapII | CCGG | 1 cut(s) | 135 |
| Hin1I | GRCGYC | 1 cut(s) | 187 |
| Hin1II | CATG | 1 cut(s) | 616 |
| HincII | GTYRAC | 1 cut(s) | 404 |
| HindII | GTYRAC | 1 cut(s) | 404 |
| HindIII | AAGCTT | 1 cut(s) | 846 |
| HinfI | GANTC | 6 cut(s) | 70, 91, 146, 201, 254, 684 |
| HpaII | CCGG | 1 cut(s) | 135 |
| HphI | GGTGA | 4 cut(s) | 65, 122, 767, 935 |
| Hpy166II | GTNNAC | 4 cut(s) | 113, 404, 534, 578 |
| Hpy188I | TCNGA | 3 cut(s) | 175, 306, 767 |
| Hpy188III | TCNNGA | 6 cut(s) | 248, 260, 262, 628, 737, 989 |
| Hpy8I | GTNNAC | 4 cut(s) | 113, 404, 534, 578 |
| Hpy99I | CGWCG | 1 cut(s) | 405 |
| HpyAV | CCTTC | 4 cut(s) | 224, 394, 595, 857 |
| HpyCH4III | ACNGT | 4 cut(s) | 194, 268, 487, 875 |
| HpyCH4IV | ACGT | 2 cut(s) | 187, 915 |
| HpyCH4V | TGCA | 6 cut(s) | 381, 467, 534, 657, 843, 956 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 128 |
| HpyF3I | CTNAG | 3 cut(s) | 719, 792, 885 |
| HpySE526I | ACGT | 2 cut(s) | 187, 915 |
| Hsp92I | GRCGYC | 1 cut(s) | 187 |
| Hsp92II | CATG | 1 cut(s) | 616 |
| Kzo9I | GATC | 6 cut(s) | 205, 322, 360, 415, 947, 983 |
| LguI | GCTCTTC | 1 cut(s) | 14 |
| LmnI | GCTCC | 1 cut(s) | 422 |
| Lsp1109I | GCAGC | 1 cut(s) | 412 |
| MaeI | CTAG | 4 cut(s) | 449, 519, 828, 945 |
| MaeII | ACGT | 2 cut(s) | 187, 915 |
| MaeIII | GTNAC | 3 cut(s) | 188, 194, 451 |
| MalI | GATC | 6 cut(s) | 207, 324, 362, 417, 949, 985 |
| MboI | GATC | 6 cut(s) | 205, 322, 360, 415, 947, 983 |
| MfeI | CAATTG | 1 cut(s) | 376 |
| MflI | RGATCY | 3 cut(s) | 322, 947, 983 |
| MhlI | GDGCHC | 1 cut(s) | 536 |
| MluCI | AATT | 3 cut(s) | 376, 564, 768 |
| MlyI | GAGTC | 1 cut(s) | 195 |
| MmeI | TCCRAC | 1 cut(s) | 840 |
| MnlI | CCTC | 8 cut(s) | 29, 46, 55, 142, 309, 413, 500, 902 |
| Mph1103I | ATGCAT | 1 cut(s) | 659 |
| MroXI | GAANNNNTTC | 2 cut(s) | 69, 145 |
| MseI | TTAA | 3 cut(s) | 102, 270, 494 |
| MslI | CAYNNNNRTG | 1 cut(s) | 785 |
| MspI | CCGG | 1 cut(s) | 135 |
| MunI | CAATTG | 1 cut(s) | 376 |
| Mva1269I | GAATGC | 1 cut(s) | 659 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 128 |
| NdeI | CATATG | 1 cut(s) | 163 |
| NdeII | GATC | 6 cut(s) | 205, 322, 360, 415, 947, 983 |
| NlaIII | CATG | 1 cut(s) | 616 |
| NlaIV | GGNNCC | 1 cut(s) | 130 |
| NmuCI | GTSAC | 2 cut(s) | 188, 451 |
| NsiI | ATGCAT | 1 cut(s) | 659 |
| PaeR7I | CTCGAG | 1 cut(s) | 260 |
| PciSI | GCTCTTC | 1 cut(s) | 14 |
| PctI | GAATGC | 1 cut(s) | 659 |
| PdmI | GAANNNNTTC | 2 cut(s) | 69, 145 |
| PfeI | GAWTC | 5 cut(s) | 70, 91, 146, 254, 684 |
| PkrI | GCNGC | 1 cut(s) | 427 |
| PleI | GAGTC | 1 cut(s) | 195 |
| PpsI | GAGTC | 1 cut(s) | 195 |
| PsiI | TTATAA | 1 cut(s) | 930 |
| PspN4I | GGNNCC | 1 cut(s) | 130 |
| PspPI | GGNCC | 1 cut(s) | 560 |
| PsuI | RGATCY | 3 cut(s) | 322, 947, 983 |
| RseI | CAYNNNNRTG | 1 cut(s) | 785 |
| SalI | GTCGAC | 1 cut(s) | 402 |
| SapI | GCTCTTC | 1 cut(s) | 14 |
| SaqAI | TTAA | 3 cut(s) | 102, 270, 494 |
| SatI | GCNGC | 1 cut(s) | 426 |
| Sau3AI | GATC | 6 cut(s) | 205, 322, 360, 415, 947, 983 |
| Sau96I | GGNCC | 1 cut(s) | 560 |
| SchI | GAGTC | 1 cut(s) | 195 |
| SduI | GDGCHC | 1 cut(s) | 536 |
| SfcI | CTRYAG | 3 cut(s) | 123, 546, 963 |
| Sfr274I | CTCGAG | 1 cut(s) | 260 |
| SinI | GGWCC | 1 cut(s) | 560 |
| SlaI | CTCGAG | 1 cut(s) | 260 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 785 |
| SmlI | CTYRAG | 1 cut(s) | 260 |
| SmoI | CTYRAG | 1 cut(s) | 260 |
| Sse9I | AATT | 3 cut(s) | 376, 564, 768 |
| SspMI | CTAG | 4 cut(s) | 449, 519, 828, 945 |
| StyI | CCWWGG | 1 cut(s) | 725 |
| TaaI | ACNGT | 4 cut(s) | 194, 268, 487, 875 |
| TaiI | ACGT | 2 cut(s) | 190, 918 |
| TaqI | TCGA | 4 cut(s) | 204, 261, 403, 418 |
| TasI | AATT | 3 cut(s) | 376, 564, 768 |
| TfiI | GAWTC | 5 cut(s) | 70, 91, 146, 254, 684 |
| Tru1I | TTAA | 3 cut(s) | 102, 270, 494 |
| Tru9I | TTAA | 3 cut(s) | 102, 270, 494 |
| TscAI | CASTG | 3 cut(s) | 159, 460, 655 |
| TseFI | GTSAC | 2 cut(s) | 188, 451 |
| TseI | GCWGC | 1 cut(s) | 425 |
| Tsp45I | GTSAC | 2 cut(s) | 188, 451 |
| TspDTI | ATGAA | 3 cut(s) | 243, 303, 688 |
| TspGWI | ACGGA | 2 cut(s) | 213, 389 |
| TspRI | CASTG | 3 cut(s) | 159, 460, 655 |
| VneI | GTGCAC | 1 cut(s) | 532 |
| VpaK11BI | GGWCC | 1 cut(s) | 560 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 795 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 83 |
| XhoI | CTCGAG | 1 cut(s) | 260 |
| XmiI | GTMKAC | 1 cut(s) | 403 |
| XmnI | GAANNNNTTC | 2 cut(s) | 69, 145 |
| XspI | CTAG | 4 cut(s) | 449, 519, 828, 945 |
| ZraI | GACGTC | 1 cut(s) | 188 |
| Zsp2I | ATGCAT | 1 cut(s) | 659 |