AT2G44270

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
Physical Location & Seq
Reverse (-)
18297878 .. 18300304
2427 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G44270.1

Sequence Viewer

Length: 1068 bp
ATGGAGGCCAAGAACAAGAAAGCAGTAGCCTCCCGTCTCTGCTGCTTATGCAACCTGAGACGTCCCGTCCTCAAAAGACCTAAAACCCTTCAACAGATATGCAGAGAGTGCTTTTATGAGGTTTTTGAGGAGGAGATTCATCAAGTCATTGTTCAGAATCGTTTATTCAAATCTGGTGAACGTGTTGCTATAGGTGCCTCTGGTGGAAAAGATTCTACTGTTCTGGCATATGTGTTATCAGAACTAAACAGACGCCACAATTATGGGCTAGACCTCTTTCTCTTGTCCATAGATGAAGGGATTACGGGTTATCGTGATGATTCTCTTGAGACTGTTAAACGGAACGAAGTCCAATATGGTTTGCCTCTTAAGATTGTTTCATATAAAGATCTGTATGGATGGACAATGGATGAGATTGTGAAAATGATTGGTTTGAAGAACAATTGCACCTTTTGTGGTGTATTCCGTCGACAGGCACTTGACCGAGGAGCTGCGTTATTGAAAGTAGAGAAGCTAGTCACTGGACATAATGCAGATGATATAGCAGAAACCGTTCTCTTGAACATATTGCGAGGGGATATTGCTAGATTAAGTAGGTGCACATCGATTACTACTGGTGAAGATGGTCCCATTCCAAGATGTAAACCTTTCAAGTATACATACGAAAAGGAGATTGTCATGTATGCTTATTTCAAGAAGCTGGATTACTTCTCCACTGAATGCATTTACTCTCCTAATGCATATCGTGGGTTTGCCCGTGAGTTCATCAAAGATTTGGAAAGAATAAGGCCAAGGGCGATTCTGGACATCATAAAGTCTGGTGAAGATTTTAGAATTGCAACAACCACAAAGATGCCTGAGCAAGGGACGTGTGAGCGATGCGGGTATATTTCTAGCCAGAAATGGTGTAAAGCTTGTGTTTTGCTGGAAGGACTGAACCGTGGTTTGCCTAAGATGGGTATTGGAAGACCTCGAGGCGTAAATGGTGATCATAATAAGGAAACAAAGAAGCCTGGATCTGTAGCAAAATCTATAGAGAGCAAACAATGTGGATCTCTGGATTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
Pfam Domains
Protein Families

Protein Analysis

355

Amino Acids

40.28

Weight (kDa)

9.08

Isoelectric Point (pI)

42.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP_bind_3 PF01171 62 - 246 2e-14 PP-loop domain
zn-ribbon_14 PF16503 289 - 319 1.7e-18 Zinc-ribbon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 64
AbsI CCTCGAGG 1 cut(s) 972
AccB1I GGYRCC 1 cut(s) 194
AccI GTMKAC 2 cut(s) 469, 656
AciI CCGC 1 cut(s) 882
AclWI GGATC 2 cut(s) 1024, 1060
AcyI GRCGYC 2 cut(s) 61, 253
AfiI CCNNNNNNNGG 3 cut(s) 472, 863, 956
AflII CTTAAG 1 cut(s) 368
AflIII ACRYGT 2 cut(s) 181, 869
AgsI TTSAA 7 cut(s) 92, 169, 436, 502, 562, 652, 694
AhdI GACNNNNNGTC 1 cut(s) 65
AjiI CACGTC 1 cut(s) 870
AjnI CCWGG 1 cut(s) 1012
AluBI AGCT 4 cut(s) 491, 514, 700, 914
AluI AGCT 4 cut(s) 491, 514, 700, 914
Alw21I GWGCWC 1 cut(s) 602
Alw26I GTCTC 3 cut(s) 41, 52, 323
Alw44I GTGCAC 1 cut(s) 598
AlwI GGATC 2 cut(s) 1024, 1060
Ama87I CYCGRG 1 cut(s) 972
AoxI GGCC 2 cut(s) 6, 788
ApaLI GTGCAC 1 cut(s) 598
ApeKI GCWGC 2 cut(s) 42, 491
Asp700I GAANNNNTTC 2 cut(s) 211, 552
AspS9I GGNCC 1 cut(s) 626
AsuHPI GGTGA 4 cut(s) 188, 629, 833, 998
AvaI CYCGRG 1 cut(s) 972
AvaII GGWCC 1 cut(s) 626
BaeGI GKGCMC 1 cut(s) 602
BanI GGYRCC 1 cut(s) 194
BarI GAAGNNNNNNTAC 2 cut(s) 689, 721
BbsI GAAGAC 1 cut(s) 973
Bbv12I GWGCWC 1 cut(s) 602
BbvI GCAGC 2 cut(s) 29, 478
BccI CCATC 3 cut(s) 393, 617, 949
BciT130I CCWGG 1 cut(s) 1014
BclI TGATCA 1 cut(s) 988
BcoDI GTCTC 3 cut(s) 41, 52, 323
BfaI CTAG 4 cut(s) 269, 515, 585, 894
BfmI CTRYAG 3 cut(s) 189, 1020, 1032
BfrI CTTAAG 1 cut(s) 368
BglII AGATCT 1 cut(s) 388
BisI GCNGC 2 cut(s) 43, 492
BlsI GCNGC 2 cut(s) 44, 493
Bme1390I CCNGG 1 cut(s) 1014
Bme18I GGWCC 1 cut(s) 626
BmeRI GACNNNNNGTC 1 cut(s) 65
BmeT110I CYCGRG 1 cut(s) 972
BmgBI CACGTC 1 cut(s) 870
BmgT120I GGNCC 1 cut(s) 626
BmiI GGNNCC 2 cut(s) 196, 628
BmrFI CCNGG 1 cut(s) 1014
BmsI GCATC 2 cut(s) 843, 869
BpiI GAAGAC 1 cut(s) 973
Bpu10I CCTNAGC 1 cut(s) 858
BpuEI CTTGAG 1 cut(s) 347
Bsa29I ATCGAT 1 cut(s) 605
BsaHI GRCGYC 2 cut(s) 61, 253
BsaJI CCNNGG 3 cut(s) 484, 791, 940
Bsc4I CCNNNNNNNGG 3 cut(s) 472, 863, 956
Bse1I ACTGG 2 cut(s) 526, 619
BseBI CCWGG 1 cut(s) 1014
BseCI ATCGAT 1 cut(s) 605
BseDI CCNNGG 3 cut(s) 484, 791, 940
BseGI GGATG 2 cut(s) 404, 415
BseLI CCNNNNNNNGG 3 cut(s) 472, 863, 956
BseMII CTCAG 2 cut(s) 47, 849
BseNI ACTGG 2 cut(s) 526, 619
BseRI GAGGAG 3 cut(s) 143, 146, 501
BseSI GKGCMC 1 cut(s) 602
BseXI GCAGC 2 cut(s) 29, 478
BshFI GGCC 2 cut(s) 8, 790
BshNI GGYRCC 1 cut(s) 194
BshVI ATCGAT 1 cut(s) 605
BsiHKAI GWGCWC 1 cut(s) 602
BsiHKCI CYCGRG 1 cut(s) 972
BslFI GGGAC 3 cut(s) 48, 612, 880
BslI CCNNNNNNNGG 3 cut(s) 472, 863, 956
BsmAI GTCTC 3 cut(s) 41, 52, 323
BsmBI CGTCTC 2 cut(s) 41, 52
BsmFI GGGAC 3 cut(s) 48, 612, 880
BsmI GAATGC 1 cut(s) 725
BsnI GGCC 2 cut(s) 8, 790
BsoBI CYCGRG 1 cut(s) 972
Bsp1286I GDGCHC 1 cut(s) 602
Bsp143I GATC 4 cut(s) 388, 988, 1016, 1052
BspACI CCGC 1 cut(s) 882
BspANI GGCC 2 cut(s) 8, 790
BspCNI CTCAG 2 cut(s) 48, 850
BspDI ATCGAT 1 cut(s) 605
BspLI GGNNCC 2 cut(s) 196, 628
BspPI GGATC 2 cut(s) 1024, 1060
BspT107I GGYRCC 1 cut(s) 194
BspTI CTTAAG 1 cut(s) 368
BsrI ACTGG 2 cut(s) 526, 619
BssECI CCNNGG 3 cut(s) 484, 791, 940
BssMI GATC 4 cut(s) 388, 988, 1016, 1052
BssNAI GTATAC 1 cut(s) 657
BssNI GRCGYC 2 cut(s) 61, 253
BssT1I CCWWGG 1 cut(s) 791
Bst1107I GTATAC 1 cut(s) 657
Bst2UI CCWGG 1 cut(s) 1014
Bst4CI ACNGT 4 cut(s) 220, 334, 553, 941
BstACI GRCGYC 2 cut(s) 61, 253
BstAFI CTTAAG 1 cut(s) 368
BstAPI GCANNNNNTGC 1 cut(s) 108
BstDEI CTNAG 3 cut(s) 56, 858, 951
BstDSI CCRYGG 1 cut(s) 940
BstENI CCTNNNNNAGG 1 cut(s) 861
BstF5I GGATG 2 cut(s) 404, 415
BstKTI GATC 4 cut(s) 391, 991, 1019, 1055
BstMAI GTCTC 3 cut(s) 41, 52, 323
BstMBI GATC 4 cut(s) 388, 988, 1016, 1052
BstMWI GCNNNNNNNGC 3 cut(s) 48, 108, 194
BstNI CCWGG 1 cut(s) 1014
BstSCI CCNGG 1 cut(s) 1012
BstSFI CTRYAG 3 cut(s) 189, 1020, 1032
BstSLI GKGCMC 1 cut(s) 602
BstV1I GCAGC 2 cut(s) 29, 478
BstV2I GAAGAC 1 cut(s) 973
BstX2I RGATCY 3 cut(s) 388, 1016, 1052
BstXI CCANNNNNNTGG 1 cut(s) 263
BstYI RGATCY 3 cut(s) 388, 1016, 1052
BstZ17I GTATAC 1 cut(s) 657
Bsu15I ATCGAT 1 cut(s) 605
BsuRI GGCC 2 cut(s) 8, 790
BsuTUI ATCGAT 1 cut(s) 605
BtgI CCRYGG 1 cut(s) 940
BtgZI GCGATG 1 cut(s) 892
BtrI CACGTC 1 cut(s) 870
BtsCI GGATG 2 cut(s) 404, 415
BtsIMutI CAGTG 2 cut(s) 519, 714
Cfr13I GGNCC 1 cut(s) 626
ClaI ATCGAT 1 cut(s) 605
CseI GACGC 1 cut(s) 261
CspCI CAANNNNNGTGG 2 cut(s) 1030, 1065
CviAII CATG 1 cut(s) 679
DdeI CTNAG 3 cut(s) 56, 858, 951
DpnI GATC 4 cut(s) 390, 990, 1018, 1054
DpnII GATC 4 cut(s) 388, 988, 1016, 1052
DriI GACNNNNNGTC 1 cut(s) 65
Eam1105I GACNNNNNGTC 1 cut(s) 65
Eco130I CCWWGG 1 cut(s) 791
Eco47I GGWCC 1 cut(s) 626
Eco88I CYCGRG 1 cut(s) 972
EcoNI CCTNNNNNAGG 1 cut(s) 861
EcoRII CCWGG 1 cut(s) 1012
EcoT14I CCWWGG 1 cut(s) 791
EcoT22I ATGCAT 2 cut(s) 725, 742
ErhI CCWWGG 1 cut(s) 791
Esp3I CGTCTC 2 cut(s) 41, 52
FaeI CATG 1 cut(s) 682
FaqI GGGAC 3 cut(s) 48, 612, 880
FatI CATG 1 cut(s) 678
FauI CCCGC 1 cut(s) 875
FauNDI CATATG 1 cut(s) 229
FbaI TGATCA 1 cut(s) 988
FblI GTMKAC 2 cut(s) 469, 656
Fnu4HI GCNGC 2 cut(s) 43, 492
FokI GGATG 2 cut(s) 411, 422
Fsp4HI GCNGC 2 cut(s) 43, 492
FspBI CTAG 4 cut(s) 269, 515, 585, 894
GluI GCNGC 2 cut(s) 43, 492
HaeIII GGCC 2 cut(s) 8, 790
HgaI GACGC 1 cut(s) 261
Hin1I GRCGYC 2 cut(s) 61, 253
Hin1II CATG 1 cut(s) 682
HincII GTYRAC 1 cut(s) 470
HindII GTYRAC 1 cut(s) 470
HindIII AAGCTT 1 cut(s) 912
HinfI GANTC 5 cut(s) 136, 157, 212, 320, 799
HphI GGTGA 4 cut(s) 188, 629, 833, 998
Hpy166II GTNNAC 5 cut(s) 179, 470, 600, 644, 657
Hpy188I TCNGA 2 cut(s) 156, 241
Hpy188III TCNNGA 6 cut(s) 314, 326, 559, 694, 803, 1058
Hpy8I GTNNAC 5 cut(s) 179, 470, 600, 644, 657
Hpy99I CGWCG 1 cut(s) 471
HpyAV CCTTC 3 cut(s) 98, 290, 923
HpyCH4III ACNGT 4 cut(s) 220, 334, 553, 941
HpyCH4IV ACGT 3 cut(s) 61, 181, 869
HpyCH4V TGCA 8 cut(s) 51, 102, 447, 533, 600, 723, 740, 839
HpyF10VI GCNNNNNNNGC 3 cut(s) 48, 108, 194
HpyF3I CTNAG 3 cut(s) 56, 858, 951
HpySE526I ACGT 3 cut(s) 61, 181, 869
Hsp92I GRCGYC 2 cut(s) 61, 253
Hsp92II CATG 1 cut(s) 682
Ksp22I TGATCA 1 cut(s) 988
Kzo9I GATC 4 cut(s) 388, 988, 1016, 1052
LmnI GCTCC 1 cut(s) 488
Lsp1109I GCAGC 2 cut(s) 29, 478
LweI GCATC 2 cut(s) 843, 869
MaeI CTAG 4 cut(s) 269, 515, 585, 894
MaeII ACGT 3 cut(s) 61, 181, 869
MaeIII GTNAC 1 cut(s) 517
MalI GATC 4 cut(s) 390, 990, 1018, 1054
MboI GATC 4 cut(s) 388, 988, 1016, 1052
MboII GAAGA 4 cut(s) 448, 632, 836, 978
MfeI CAATTG 1 cut(s) 442
MflI RGATCY 3 cut(s) 388, 1016, 1052
MhlI GDGCHC 1 cut(s) 602
MluCI AATT 3 cut(s) 259, 442, 834
Mph1103I ATGCAT 2 cut(s) 725, 742
MroXI GAANNNNTTC 2 cut(s) 211, 552
MseI TTAA 3 cut(s) 336, 369, 590
MslI CAYNNNNRTG 2 cut(s) 261, 851
MspCI CTTAAG 1 cut(s) 368
MspR9I CCNGG 1 cut(s) 1014
MunI CAATTG 1 cut(s) 442
Mva1269I GAATGC 1 cut(s) 725
MvaI CCWGG 1 cut(s) 1014
MwoI GCNNNNNNNGC 3 cut(s) 48, 108, 194
NdeI CATATG 1 cut(s) 229
NdeII GATC 4 cut(s) 388, 988, 1016, 1052
NlaIII CATG 1 cut(s) 682
NlaIV GGNNCC 2 cut(s) 196, 628
NmuCI GTSAC 1 cut(s) 517
NsiI ATGCAT 2 cut(s) 725, 742
PaeR7I CTCGAG 1 cut(s) 972
PctI GAATGC 1 cut(s) 725
PdmI GAANNNNTTC 2 cut(s) 211, 552
PfeI GAWTC 5 cut(s) 136, 157, 212, 320, 799
PkrI GCNGC 2 cut(s) 44, 493
Psp6I CCWGG 1 cut(s) 1012
PspGI CCWGG 1 cut(s) 1012
PspN4I GGNNCC 2 cut(s) 196, 628
PspPI GGNCC 1 cut(s) 626
PspXI VCTCGAGB 1 cut(s) 972
PsuI RGATCY 3 cut(s) 388, 1016, 1052
RseI CAYNNNNRTG 2 cut(s) 261, 851
SalI GTCGAC 1 cut(s) 468
SaqAI TTAA 3 cut(s) 336, 369, 590
SatI GCNGC 2 cut(s) 43, 492
Sau3AI GATC 4 cut(s) 388, 988, 1016, 1052
Sau96I GGNCC 1 cut(s) 626
ScrFI CCNGG 1 cut(s) 1014
SduI GDGCHC 1 cut(s) 602
SfaNI GCATC 2 cut(s) 843, 869
SfcI CTRYAG 3 cut(s) 189, 1020, 1032
Sfr274I CTCGAG 1 cut(s) 972
SinI GGWCC 1 cut(s) 626
SlaI CTCGAG 1 cut(s) 972
SmiMI CAYNNNNRTG 2 cut(s) 261, 851
SmlI CTYRAG 3 cut(s) 326, 368, 972
SmoI CTYRAG 3 cut(s) 326, 368, 972
Sse9I AATT 3 cut(s) 259, 442, 834
SsiI CCGC 1 cut(s) 882
SspMI CTAG 4 cut(s) 269, 515, 585, 894
StyD4I CCNGG 1 cut(s) 1012
StyI CCWWGG 1 cut(s) 791
TaaI ACNGT 4 cut(s) 220, 334, 553, 941
TaiI ACGT 3 cut(s) 64, 184, 872
TaqI TCGA 3 cut(s) 469, 605, 973
TaqII GACCGA 1 cut(s) 498
TasI AATT 3 cut(s) 259, 442, 834
TfiI GAWTC 5 cut(s) 136, 157, 212, 320, 799
Tru1I TTAA 3 cut(s) 336, 369, 590
Tru9I TTAA 3 cut(s) 336, 369, 590
TscAI CASTG 2 cut(s) 526, 721
TseFI GTSAC 1 cut(s) 517
TseI GCWGC 2 cut(s) 42, 491
Tsp45I GTSAC 1 cut(s) 517
TspDTI ATGAA 4 cut(s) 128, 309, 369, 754
TspGWI ACGGA 2 cut(s) 355, 455
TspRI CASTG 2 cut(s) 526, 721
Vha464I CTTAAG 1 cut(s) 368
VneI GTGCAC 1 cut(s) 598
VpaK11BI GGWCC 1 cut(s) 626
XagI CCTNNNNNAGG 1 cut(s) 861
XhoI CTCGAG 1 cut(s) 972
XmiI GTMKAC 2 cut(s) 469, 656
XmnI GAANNNNTTC 2 cut(s) 211, 552
XspI CTAG 4 cut(s) 269, 515, 585, 894
ZraI GACGTC 1 cut(s) 62
Zsp2I ATGCAT 2 cut(s) 725, 742
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.