Prupe.1G356900_v2.0.a1

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0005079
Physical Location & Seq
Forward (+)
33058538 .. 33061834
3297 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G356900.1

Sequence Viewer

Length: 1074 bp
ATGGAGGAGTCAGCCGACGGCAAGGCCAAGAAGGCTGGGGCTCGCCTGTGCTGCCTCTGTCATCAGAAAAAAGCTGCCCTGAAGAGGCCCAAAACCCTAGAGCAGATTTGCAGGGAGTGTTTCTATGAGGTATTTGAGGAGGAGATCCATCAGGTAATTGTCGAAAACCAGTTATTTAAGCCTGGTGAGCGCATTGCCCTTGGCGCCTCTGGTGGGAAAGATTCCACTGTCCTTGCTTATGTGTTATCGGAATTGAATAGGCGGCACAATTACGGCCTGGATCTCTTCCTGTTGTCAGTGGATGAGGGCATTACTGGCTACAGGGATGATTCTCTTGAGACCGTCAAAAGAAATGAAATCCAGTATGGTTTGCCACTAAAAATAGTCTCGTACAAGGATTTGTATGGGTGGACCATGGATGAAATAGTTAAGATGATTGGTCTCAAGAATAATTGCACCTTCTGTGGTGTTTTTCGTCGTCAGGCCCTCGATCGCGGTGCTGCATTGTTGAAGGTAGACAAGCTCGCTACTGGACATAATGCAGATGATATTGCTGAAACAGTTCTGTTGAACATATTACGAGGTGACATTGCAAGATTGAGTAGATGCACTTCAATTGTCACTGGTGAAGATGGGCCAATTCCAAGATGCAAACCTTTTAAATACACCTACGAGAAAGAGATTGTTATGTATGCATACTTCAAGAGGCTGGACTACTTCTCCACCGAATGCATTTATTCACCCAATGCTTATCGTGGTTTTGCTCGCGAGTTCATCAAGGATTTGGAAAGAATAAGACCTAGAGCCATACTTGACATCATCAAATCAGGAGAGAACTTCAGAATTTCCACTTCTACAAAAATGCCAGAGCAGGGGACATGTGAACGGTGTGGTTACATTTCAAGCCAGAAATGGTGTAAGGCCTGTGTTTTGCTTGAGGGACTGAATAGGGGTTTGCCAAAGCTAGGAATTGGACGGACTCGTGGACTTAATAACGATGATAAGAAGGATACAAAGAAAACTAATGGAACCAAAAGCATCGAAAGCAAACAATGTGGAACTTTGGACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04122 map04122
Pfam Domains
Protein Families
ATP_bind_3 zn-ribbon_14

Protein Analysis

358

Amino Acids

40.42

Weight (kDa)

8.83

Isoelectric Point (pI)

33.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 203
AccI GTMKAC 1 cut(s) 516
AccII CGCG 2 cut(s) 495, 768
AciI CCGC 2 cut(s) 262, 495
AclWI GGATC 2 cut(s) 139, 288
AcsI RAATTY 1 cut(s) 843
AcuI CTGAAG 2 cut(s) 101, 823
AcyI GRCGYC 1 cut(s) 204
AfaI GTAC 1 cut(s) 392
AfiI CCNNNNNNNGG 4 cut(s) 84, 213, 872, 965
AflIII ACRYGT 1 cut(s) 878
AgsI TTSAA 6 cut(s) 256, 511, 571, 615, 703, 903
AjnI CCWGG 2 cut(s) 181, 276
AluBI AGCT 3 cut(s) 74, 523, 964
AluI AGCT 3 cut(s) 74, 523, 964
Alw26I GTCTC 3 cut(s) 332, 391, 446
AlwI GGATC 2 cut(s) 139, 288
AoxI GGCC 6 cut(s) 24, 86, 274, 483, 635, 921
ApeKI GCWGC 3 cut(s) 51, 74, 500
ApoI RAATTY 1 cut(s) 843
Asp700I GAANNNNTTC 1 cut(s) 561
AspLEI GCGC 2 cut(s) 192, 206
AspS9I GGNCC 4 cut(s) 87, 411, 484, 635
AsuHPI GGTGA 4 cut(s) 197, 596, 638, 732
AvaII GGWCC 1 cut(s) 411
BanI GGYRCC 1 cut(s) 203
BanII GRGCYC 1 cut(s) 43
BarI GAAGNNNNNNTAC 4 cut(s) 595, 627, 683, 715
BauI CACGAG 1 cut(s) 981
BbvI GCAGC 3 cut(s) 38, 61, 487
BccI CCATC 2 cut(s) 156, 626
BceAI ACGGC 2 cut(s) 34, 289
BcgI CGANNNNNNTGC 2 cut(s) 479, 513
BciT130I CCWGG 2 cut(s) 183, 278
BciVI GTATCC 1 cut(s) 1003
BcoDI GTCTC 3 cut(s) 332, 391, 446
BfaI CTAG 3 cut(s) 98, 801, 965
BfmI CTRYAG 1 cut(s) 319
BfoI RGCGCY 1 cut(s) 207
BfuI GTATCC 1 cut(s) 1003
BglI GCCNNNNNGGC 1 cut(s) 32
BisI GCNGC 4 cut(s) 52, 75, 263, 501
BlsI GCNGC 4 cut(s) 53, 76, 264, 502
Bme1390I CCNGG 2 cut(s) 183, 278
Bme18I GGWCC 1 cut(s) 411
BmgT120I GGNCC 4 cut(s) 87, 411, 484, 635
BmiI GGNNCC 2 cut(s) 205, 1030
BmrFI CCNGG 2 cut(s) 183, 278
BmsI GCATC 3 cut(s) 596, 638, 1047
BpuEI CTTGAG 3 cut(s) 356, 428, 956
BsaHI GRCGYC 1 cut(s) 204
BsaI GGTCTC 2 cut(s) 332, 446
BsaJI CCNNGG 2 cut(s) 199, 414
BsaXI ACNNNNNCTCC 2 cut(s) 704, 734
Bsc4I CCNNNNNNNGG 4 cut(s) 84, 213, 872, 965
Bse1I ACTGG 5 cut(s) 169, 319, 361, 535, 628
Bse3DI GCAATG 2 cut(s) 192, 588
BseBI CCWGG 2 cut(s) 183, 278
BseDI CCNNGG 2 cut(s) 199, 414
BseGI GGATG 3 cut(s) 307, 331, 424
BseLI CCNNNNNNNGG 4 cut(s) 84, 213, 872, 965
BseMI GCAATG 2 cut(s) 192, 588
BseNI ACTGG 5 cut(s) 169, 319, 361, 535, 628
BseRI GAGGAG 3 cut(s) 20, 152, 155
BseXI GCAGC 3 cut(s) 38, 61, 487
BseYI CCCAGC 1 cut(s) 35
Bsh1236I CGCG 2 cut(s) 495, 768
Bsh1285I CGRYCG 1 cut(s) 493
BshFI GGCC 6 cut(s) 26, 88, 276, 485, 637, 923
BshNI GGYRCC 1 cut(s) 203
BsiEI CGRYCG 1 cut(s) 493
BslFI GGGAC 2 cut(s) 889, 954
BslI CCNNNNNNNGG 4 cut(s) 84, 213, 872, 965
BsmAI GTCTC 3 cut(s) 332, 391, 446
BsmFI GGGAC 2 cut(s) 889, 954
BsmI GAATGC 1 cut(s) 734
BsnI GGCC 6 cut(s) 26, 88, 276, 485, 637, 923
Bso31I GGTCTC 2 cut(s) 332, 446
Bsp1286I GDGCHC 1 cut(s) 43
Bsp143I GATC 3 cut(s) 144, 280, 490
Bsp19I CCATGG 1 cut(s) 414
Bsp68I TCGCGA 1 cut(s) 768
BspACI CCGC 2 cut(s) 262, 495
BspANI GGCC 6 cut(s) 26, 88, 276, 485, 637, 923
BspFNI CGCG 2 cut(s) 495, 768
BspLI GGNNCC 2 cut(s) 205, 1030
BspPI GGATC 2 cut(s) 139, 288
BspT107I GGYRCC 1 cut(s) 203
BspTNI GGTCTC 2 cut(s) 332, 446
BsrDI GCAATG 2 cut(s) 192, 588
BsrI ACTGG 5 cut(s) 169, 319, 361, 535, 628
BssECI CCNNGG 2 cut(s) 199, 414
BssMI GATC 3 cut(s) 144, 280, 490
BssNI GRCGYC 1 cut(s) 204
BssSI CACGAG 1 cut(s) 981
BssT1I CCWWGG 2 cut(s) 199, 414
Bst2BI CACGAG 1 cut(s) 981
Bst2UI CCWGG 2 cut(s) 183, 278
Bst4CI ACNGT 4 cut(s) 229, 343, 562, 888
Bst6I CTCTTC 2 cut(s) 77, 290
BstACI GRCGYC 1 cut(s) 204
BstC8I GCNNGC 3 cut(s) 43, 525, 766
BstDSI CCRYGG 1 cut(s) 414
BstF5I GGATG 3 cut(s) 307, 331, 424
BstFNI CGCG 2 cut(s) 495, 768
BstH2I RGCGCY 1 cut(s) 207
BstHHI GCGC 2 cut(s) 192, 206
BstKTI GATC 3 cut(s) 147, 283, 493
BstMAI GTCTC 3 cut(s) 332, 391, 446
BstMBI GATC 3 cut(s) 144, 280, 490
BstMCI CGRYCG 1 cut(s) 493
BstMWI GCNNNNNNNGC 6 cut(s) 32, 51, 187, 203, 315, 1044
BstNI CCWGG 2 cut(s) 183, 278
BstNSI RCATGY 1 cut(s) 882
BstSCI CCNGG 2 cut(s) 181, 276
BstSFI CTRYAG 1 cut(s) 319
BstUI CGCG 2 cut(s) 495, 768
BstV1I GCAGC 3 cut(s) 38, 61, 487
BstX2I RGATCY 2 cut(s) 144, 280
BstYI RGATCY 2 cut(s) 144, 280
BsuI GTATCC 1 cut(s) 1003
BsuRI GGCC 6 cut(s) 26, 88, 276, 485, 637, 923
BtgI CCRYGG 1 cut(s) 414
BtsCI GGATG 3 cut(s) 307, 331, 424
BtsIMutI CAGTG 3 cut(s) 225, 303, 621
BtuMI TCGCGA 1 cut(s) 768
Cac8I GCNNGC 3 cut(s) 43, 525, 766
CfoI GCGC 2 cut(s) 192, 206
Cfr13I GGNCC 4 cut(s) 87, 411, 484, 635
Csp6I GTAC 1 cut(s) 391
CspCI CAANNNNNGTGG 4 cut(s) 214, 249, 1036, 1071
CviAII CATG 2 cut(s) 415, 879
CviQI GTAC 1 cut(s) 391
DinI GGCGCC 1 cut(s) 205
DpnI GATC 3 cut(s) 146, 282, 492
DpnII GATC 3 cut(s) 144, 280, 490
DraI TTTAAA 1 cut(s) 661
Eam1104I CTCTTC 2 cut(s) 77, 290
EarI CTCTTC 2 cut(s) 77, 290
Eco130I CCWWGG 2 cut(s) 199, 414
Eco147I AGGCCT 1 cut(s) 923
Eco24I GRGCYC 1 cut(s) 43
Eco31I GGTCTC 2 cut(s) 332, 446
Eco47I GGWCC 1 cut(s) 411
Eco57I CTGAAG 2 cut(s) 101, 823
EcoO109I RGGNCCY 1 cut(s) 484
EcoRII CCWGG 2 cut(s) 181, 276
EcoT14I CCWWGG 2 cut(s) 199, 414
EcoT22I ATGCAT 2 cut(s) 697, 734
EcoT38I GRGCYC 1 cut(s) 43
EgeI GGCGCC 1 cut(s) 205
EheI GGCGCC 1 cut(s) 205
ErhI CCWWGG 2 cut(s) 199, 414
FaeI CATG 2 cut(s) 418, 882
FaqI GGGAC 2 cut(s) 889, 954
FatI CATG 2 cut(s) 414, 878
FblI GTMKAC 1 cut(s) 516
Fnu4HI GCNGC 4 cut(s) 52, 75, 263, 501
FokI GGATG 3 cut(s) 314, 338, 431
FriOI GRGCYC 1 cut(s) 43
Fsp4HI GCNGC 4 cut(s) 52, 75, 263, 501
FspBI CTAG 3 cut(s) 98, 801, 965
GlaI GCGC 2 cut(s) 191, 205
GluI GCNGC 4 cut(s) 52, 75, 263, 501
GsaI CCCAGC 1 cut(s) 39
HaeII RGCGCY 1 cut(s) 207
HaeIII GGCC 6 cut(s) 26, 88, 276, 485, 637, 923
HhaI GCGC 2 cut(s) 192, 206
Hin1I GRCGYC 1 cut(s) 204
Hin1II CATG 2 cut(s) 418, 882
Hin6I GCGC 2 cut(s) 190, 204
HinP1I GCGC 2 cut(s) 190, 204
HinfI GANTC 4 cut(s) 8, 221, 329, 979
HphI GGTGA 4 cut(s) 197, 596, 638, 732
Hpy166II GTNNAC 4 cut(s) 411, 517, 884, 986
Hpy188I TCNGA 4 cut(s) 66, 250, 842, 1073
Hpy188III TCNNGA 5 cut(s) 335, 445, 703, 767, 828
Hpy8I GTNNAC 4 cut(s) 411, 517, 884, 986
Hpy99I CGWCG 2 cut(s) 20, 480
HpyAV CCTTC 4 cut(s) 25, 469, 505, 1000
HpyCH4III ACNGT 4 cut(s) 229, 343, 562, 888
HpyCH4V TGCA 9 cut(s) 111, 456, 503, 542, 593, 609, 651, 695, 732
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 51, 187, 203, 315, 1044
Hsp92I GRCGYC 1 cut(s) 204
Hsp92II CATG 2 cut(s) 418, 882
HspAI GCGC 2 cut(s) 190, 204
KasI GGCGCC 1 cut(s) 203
Kzo9I GATC 3 cut(s) 144, 280, 490
Lsp1109I GCAGC 3 cut(s) 38, 61, 487
LweI GCATC 3 cut(s) 596, 638, 1047
MaeI CTAG 3 cut(s) 98, 801, 965
MaeIII GTNAC 3 cut(s) 584, 619, 893
MalI GATC 3 cut(s) 146, 282, 492
MboI GATC 3 cut(s) 144, 280, 490
MboII GAAGA 3 cut(s) 94, 277, 641
MfeI CAATTG 1 cut(s) 615
MflI RGATCY 2 cut(s) 144, 280
MhlI GDGCHC 1 cut(s) 43
MluCI AATT 8 cut(s) 156, 251, 268, 451, 615, 639, 843, 969
Mly113I GGCGCC 1 cut(s) 204
MlyI GAGTC 2 cut(s) 17, 973
Mph1103I ATGCAT 2 cut(s) 697, 734
MroXI GAANNNNTTC 1 cut(s) 561
MseI TTAA 4 cut(s) 177, 429, 660, 990
MspR9I CCNGG 2 cut(s) 183, 278
MunI CAATTG 1 cut(s) 615
Mva1269I GAATGC 1 cut(s) 734
MvaI CCWGG 2 cut(s) 183, 278
MvnI CGCG 2 cut(s) 495, 768
MwoI GCNNNNNNNGC 6 cut(s) 32, 51, 187, 203, 315, 1044
NarI GGCGCC 1 cut(s) 204
NcoI CCATGG 1 cut(s) 414
NdeII GATC 3 cut(s) 144, 280, 490
NlaIII CATG 2 cut(s) 418, 882
NlaIV GGNNCC 2 cut(s) 205, 1030
NmuCI GTSAC 2 cut(s) 584, 619
NruI TCGCGA 1 cut(s) 768
NsiI ATGCAT 2 cut(s) 697, 734
NspI RCATGY 1 cut(s) 882
PceI AGGCCT 1 cut(s) 923
PciI ACATGT 1 cut(s) 878
PctI GAATGC 1 cut(s) 734
PdmI GAANNNNTTC 1 cut(s) 561
PfeI GAWTC 2 cut(s) 221, 329
PkrI GCNGC 4 cut(s) 53, 76, 264, 502
Ple19I CGATCG 1 cut(s) 493
PleI GAGTC 2 cut(s) 16, 973
PluTI GGCGCC 1 cut(s) 207
PpsI GAGTC 2 cut(s) 16, 973
PscI ACATGT 1 cut(s) 878
Psp6I CCWGG 2 cut(s) 181, 276
PspFI CCCAGC 1 cut(s) 35
PspGI CCWGG 2 cut(s) 181, 276
PspN4I GGNNCC 2 cut(s) 205, 1030
PspPI GGNCC 4 cut(s) 87, 411, 484, 635
PsuI RGATCY 2 cut(s) 144, 280
PvuI CGATCG 1 cut(s) 493
RruI TCGCGA 1 cut(s) 768
RsaI GTAC 1 cut(s) 392
RsaNI GTAC 1 cut(s) 391
SaqAI TTAA 4 cut(s) 177, 429, 660, 990
SatI GCNGC 4 cut(s) 52, 75, 263, 501
Sau3AI GATC 3 cut(s) 144, 280, 490
Sau96I GGNCC 4 cut(s) 87, 411, 484, 635
SchI GAGTC 2 cut(s) 17, 973
ScrFI CCNGG 2 cut(s) 183, 278
SduI GDGCHC 1 cut(s) 43
SfaNI GCATC 3 cut(s) 596, 638, 1047
SfcI CTRYAG 1 cut(s) 319
SfoI GGCGCC 1 cut(s) 205
SinI GGWCC 1 cut(s) 411
SmlI CTYRAG 3 cut(s) 335, 443, 935
SmoI CTYRAG 3 cut(s) 335, 443, 935
Sse9I AATT 8 cut(s) 156, 251, 268, 451, 615, 639, 843, 969
SseBI AGGCCT 1 cut(s) 923
SsiI CCGC 2 cut(s) 262, 495
SspDI GGCGCC 1 cut(s) 203
SspMI CTAG 3 cut(s) 98, 801, 965
StuI AGGCCT 1 cut(s) 923
StyD4I CCNGG 2 cut(s) 181, 276
StyI CCWWGG 2 cut(s) 199, 414
TaaI ACNGT 4 cut(s) 229, 343, 562, 888
TaqI TCGA 3 cut(s) 162, 489, 1041
TasI AATT 8 cut(s) 156, 251, 268, 451, 615, 639, 843, 969
TauI GCSGC 1 cut(s) 265
TfiI GAWTC 2 cut(s) 221, 329
Tru1I TTAA 4 cut(s) 177, 429, 660, 990
Tru9I TTAA 4 cut(s) 177, 429, 660, 990
TscAI CASTG 3 cut(s) 232, 303, 628
TseFI GTSAC 2 cut(s) 584, 619
TseI GCWGC 3 cut(s) 51, 74, 500
Tsp45I GTSAC 2 cut(s) 584, 619
TspDTI ATGAA 3 cut(s) 369, 435, 763
TspGWI ACGGA 1 cut(s) 991
TspRI CASTG 3 cut(s) 232, 303, 628
VpaK11BI GGWCC 1 cut(s) 411
XapI RAATTY 1 cut(s) 843
XceI RCATGY 1 cut(s) 882
XmiI GTMKAC 1 cut(s) 516
XmnI GAANNNNTTC 1 cut(s) 561
XspI CTAG 3 cut(s) 98, 801, 965
Zsp2I ATGCAT 2 cut(s) 697, 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.