Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G76170 AT1G76170 AT1G76170 AT1G76170 AT1G76170 AT1G76170 AT1G76170 AT2G44270 |
| fragaria_vesca | FvH4_2g37771 FvH4_2g37771 FvH4_2g37771 |
| malus_domestica | MD08G1004800.v1.1 MD15G1004300.v1.1 |
| prunus_persica | Prupe.1G356900_v2.0.a1 |
| pyrus_communis | pycom08g00360 pycom08g00370 pycom15g00310 pycom15g00320 |
| rosa_chinensis | RchiOBHm_Chr6g0303951 RchiOBHm_Chr6g0303961 |
| rosa_laevigata | RLG00000011030 |
| rosa_multiflora | Rmu_sc0004644.1_g000013 Rmu_sc0004644.1_g000014 |
| rosa_roxburghii | Rroxscaffold_7G00164230 |
| rosa_rugosa | Rorug06G0326200 |
| rosa_samantha | Rh6AG440500 Rh6BG462400 Rh6CG452800 Rh6DG439800 |
| rosa_wichuraiana | Rw6G038150 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 2 cut(s) | 127, 135 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 164, 257 |
| AflIII | ACRYGT | 1 cut(s) | 170 |
| AgsI | TTSAA | 1 cut(s) | 195 |
| AluBI | AGCT | 1 cut(s) | 215 |
| AluI | AGCT | 1 cut(s) | 215 |
| AoxI | GGCC | 1 cut(s) | 96 |
| ApoI | RAATTY | 2 cut(s) | 127, 135 |
| AspA2I | CCTAGG | 1 cut(s) | 92 |
| AspS9I | GGNCC | 1 cut(s) | 96 |
| AsuHPI | GGTGA | 1 cut(s) | 24 |
| AvrII | CCTAGG | 1 cut(s) | 92 |
| BauI | CACGAG | 1 cut(s) | 57 |
| BccI | CCATC | 1 cut(s) | 250 |
| BfaI | CTAG | 1 cut(s) | 93 |
| BlnI | CCTAGG | 1 cut(s) | 92 |
| BmgT120I | GGNCC | 1 cut(s) | 96 |
| BpuEI | CTTGAG | 1 cut(s) | 248 |
| BsaJI | CCNNGG | 1 cut(s) | 92 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 164, 257 |
| BseDI | CCNNGG | 1 cut(s) | 92 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 164, 257 |
| BseMII | CTCAG | 1 cut(s) | 305 |
| BshFI | GGCC | 1 cut(s) | 98 |
| BsiSI | CCGG | 1 cut(s) | 158 |
| BslFI | GGGAC | 2 cut(s) | 181, 246 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 164, 257 |
| BsmFI | GGGAC | 2 cut(s) | 181, 246 |
| BsmI | GAATGC | 1 cut(s) | 26 |
| BsnI | GGCC | 1 cut(s) | 98 |
| BspANI | GGCC | 1 cut(s) | 98 |
| BspCNI | CTCAG | 1 cut(s) | 304 |
| BssECI | CCNNGG | 1 cut(s) | 92 |
| BssSI | CACGAG | 1 cut(s) | 57 |
| BssT1I | CCWWGG | 1 cut(s) | 92 |
| Bst2BI | CACGAG | 1 cut(s) | 57 |
| BstDEI | CTNAG | 1 cut(s) | 291 |
| BstNSI | RCATGY | 1 cut(s) | 174 |
| BsuRI | GGCC | 1 cut(s) | 98 |
| BtsIMutI | CAGTG | 1 cut(s) | 15 |
| Cfr13I | GGNCC | 1 cut(s) | 96 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 328, 363 |
| CviAII | CATG | 1 cut(s) | 171 |
| CviJI | RGCY | 3 cut(s) | 98, 198, 215 |
| CviKI_1 | RGCY | 3 cut(s) | 98, 198, 215 |
| DdeI | CTNAG | 1 cut(s) | 291 |
| Eco130I | CCWWGG | 1 cut(s) | 92 |
| EcoT14I | CCWWGG | 1 cut(s) | 92 |
| EcoT22I | ATGCAT | 1 cut(s) | 26 |
| ErhI | CCWWGG | 1 cut(s) | 92 |
| FaeI | CATG | 1 cut(s) | 174 |
| FaiI | YATR | 5 cut(s) | 101, 110, 172, 305, 332 |
| FaqI | GGGAC | 2 cut(s) | 181, 246 |
| FatI | CATG | 1 cut(s) | 170 |
| FspBI | CTAG | 1 cut(s) | 93 |
| HaeIII | GGCC | 1 cut(s) | 98 |
| HapII | CCGG | 1 cut(s) | 158 |
| Hin1II | CATG | 1 cut(s) | 174 |
| HindIII | AAGCTT | 1 cut(s) | 213 |
| HinfI | GANTC | 3 cut(s) | 84, 238, 271 |
| HpaII | CCGG | 1 cut(s) | 158 |
| HphI | GGTGA | 1 cut(s) | 24 |
| Hpy166II | GTNNAC | 1 cut(s) | 176 |
| Hpy188I | TCNGA | 5 cut(s) | 89, 134, 270, 294, 365 |
| Hpy188III | TCNNGA | 1 cut(s) | 59 |
| Hpy8I | GTNNAC | 1 cut(s) | 176 |
| HpyAV | CCTTC | 1 cut(s) | 292 |
| HpyCH4V | TGCA | 1 cut(s) | 24 |
| HpyF3I | CTNAG | 1 cut(s) | 291 |
| Hsp92II | CATG | 1 cut(s) | 174 |
| LpnPI | CCDG | 4 cut(s) | 105, 149, 171, 212 |
| MaeI | CTAG | 1 cut(s) | 93 |
| MaeIII | GTNAC | 1 cut(s) | 185 |
| MboII | GAAGA | 1 cut(s) | 307 |
| MluCI | AATT | 4 cut(s) | 127, 135, 261, 279 |
| MmeI | TCCRAC | 1 cut(s) | 248 |
| MnlI | CCTC | 2 cut(s) | 223, 269 |
| Mph1103I | ATGCAT | 1 cut(s) | 26 |
| MseI | TTAA | 1 cut(s) | 282 |
| MspI | CCGG | 1 cut(s) | 158 |
| Mva1269I | GAATGC | 1 cut(s) | 26 |
| NlaIII | CATG | 1 cut(s) | 174 |
| NsiI | ATGCAT | 1 cut(s) | 26 |
| NspI | RCATGY | 1 cut(s) | 174 |
| PciI | ACATGT | 1 cut(s) | 170 |
| PctI | GAATGC | 1 cut(s) | 26 |
| PfeI | GAWTC | 3 cut(s) | 84, 238, 271 |
| PscI | ACATGT | 1 cut(s) | 170 |
| PspPI | GGNCC | 1 cut(s) | 96 |
| SaqAI | TTAA | 1 cut(s) | 282 |
| Sau96I | GGNCC | 1 cut(s) | 96 |
| SetI | ASST | 2 cut(s) | 94, 217 |
| SmlI | CTYRAG | 1 cut(s) | 227 |
| SmoI | CTYRAG | 1 cut(s) | 227 |
| Sse9I | AATT | 4 cut(s) | 127, 135, 261, 279 |
| SspMI | CTAG | 1 cut(s) | 93 |
| StyI | CCWWGG | 1 cut(s) | 92 |
| TaqI | TCGA | 1 cut(s) | 274 |
| TasI | AATT | 4 cut(s) | 127, 135, 261, 279 |
| TfiI | GAWTC | 3 cut(s) | 84, 238, 271 |
| Tru1I | TTAA | 1 cut(s) | 282 |
| Tru9I | TTAA | 1 cut(s) | 282 |
| TscAI | CASTG | 1 cut(s) | 22 |
| TspDTI | ATGAA | 2 cut(s) | 55, 320 |
| TspRI | CASTG | 1 cut(s) | 22 |
| XapI | RAATTY | 2 cut(s) | 127, 135 |
| XceI | RCATGY | 1 cut(s) | 174 |
| XmaJI | CCTAGG | 1 cut(s) | 92 |
| XspI | CTAG | 1 cut(s) | 93 |
| Zsp2I | ATGCAT | 1 cut(s) | 26 |