pycom08g00360

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0005079
Physical Location & Seq
Forward (+)
344203 .. 344947
745 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g00360.1

Sequence Viewer

Length: 384 bp
ATGGAGTCGTCAGCCGACGGCAAGCAGAAGAAGGCGGGGGGCCGCCAGTGCTGCATCTGTCATGAGAGACGGGCCGCCCTCAAGAGACCCAAAACCCTAGAGCAGATTTGCAGGGAGTGTTTCTACGAGGTGTTTGAGGAGGAGATTCATCATGTTATTGTCGAAAACCAGTTATTTAAGCCTGGTGAGCGCATTGCCCTCGGTGCCTCCGGCGGCAAAGATTCCACTGTCCTCGCTTACGTATTATCCGAATTGAATAGGCGCCACAATTACGGTCTGGATCGATCTTCCTTATCTTGTCAGTGGATGAGGGCATTACTGGCTACAGGGACGATTCTCTTGAAACCGTCAAAAGAAATGAAATCCAGTATGGTTTGCCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04122 map04122
Pfam Domains
Protein Families
ATP_bind_3 zn-ribbon_14

Protein Analysis

128

Amino Acids

14.36

Weight (kDa)

9.05

Isoelectric Point (pI)

56.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 203, 261
AciI CCGC 5 cut(s) 35, 43, 75, 213, 379
AclWI GGATC 1 cut(s) 288
AcyI GRCGYC 1 cut(s) 262
AgsI TTSAA 2 cut(s) 256, 343
AjnI CCWGG 1 cut(s) 181
Alw26I GTCTC 2 cut(s) 61, 79
AlwI GGATC 1 cut(s) 288
AoxI GGCC 2 cut(s) 40, 72
ApeKI GCWGC 1 cut(s) 51
AspLEI GCGC 2 cut(s) 192, 264
AspS9I GGNCC 2 cut(s) 40, 72
AsuHPI GGTGA 1 cut(s) 197
BanI GGYRCC 2 cut(s) 203, 261
BbvI GCAGC 1 cut(s) 38
BceAI ACGGC 1 cut(s) 34
BcgI CGANNNNNNTGC 2 cut(s) 181, 215
BciT130I CCWGG 1 cut(s) 183
BcoDI GTCTC 2 cut(s) 61, 79
BfaI CTAG 1 cut(s) 98
BfmI CTRYAG 1 cut(s) 324
BfoI RGCGCY 1 cut(s) 265
BisI GCNGC 5 cut(s) 43, 52, 75, 214, 379
BlsI GCNGC 5 cut(s) 44, 53, 76, 215, 380
Bme1390I CCNGG 1 cut(s) 183
BmgT120I GGNCC 2 cut(s) 40, 72
BmiI GGNNCC 3 cut(s) 41, 205, 263
BmrFI CCNGG 1 cut(s) 183
BmsI GCATC 1 cut(s) 63
BpuEI CTTGAG 1 cut(s) 65
Bsa29I ATCGAT 1 cut(s) 283
BsaAI YACGTR 1 cut(s) 241
BsaHI GRCGYC 1 cut(s) 262
BsaI GGTCTC 1 cut(s) 79
BsaJI CCNNGG 1 cut(s) 199
Bse1I ACTGG 4 cut(s) 46, 169, 324, 366
Bse3DI GCAATG 1 cut(s) 192
BseBI CCWGG 1 cut(s) 183
BseCI ATCGAT 1 cut(s) 283
BseDI CCNNGG 1 cut(s) 199
BseGI GGATG 1 cut(s) 312
BseMI GCAATG 1 cut(s) 192
BseNI ACTGG 4 cut(s) 46, 169, 324, 366
BseRI GAGGAG 2 cut(s) 152, 155
BseXI GCAGC 1 cut(s) 38
BshFI GGCC 2 cut(s) 42, 74
BshNI GGYRCC 2 cut(s) 203, 261
BshVI ATCGAT 1 cut(s) 283
BsiSI CCGG 1 cut(s) 210
BslFI GGGAC 1 cut(s) 343
BsmAI GTCTC 2 cut(s) 61, 79
BsmBI CGTCTC 1 cut(s) 61
BsmFI GGGAC 1 cut(s) 343
BsnI GGCC 2 cut(s) 42, 74
Bso31I GGTCTC 1 cut(s) 79
Bsp143I GATC 2 cut(s) 280, 284
BspACI CCGC 5 cut(s) 35, 43, 75, 213, 379
BspANI GGCC 2 cut(s) 42, 74
BspDI ATCGAT 1 cut(s) 283
BspHI TCATGA 1 cut(s) 61
BspLI GGNNCC 3 cut(s) 41, 205, 263
BspPI GGATC 1 cut(s) 288
BspT107I GGYRCC 2 cut(s) 203, 261
BspTNI GGTCTC 1 cut(s) 79
BsrDI GCAATG 1 cut(s) 192
BsrI ACTGG 4 cut(s) 46, 169, 324, 366
BssECI CCNNGG 1 cut(s) 199
BssMI GATC 2 cut(s) 280, 284
BssNI GRCGYC 1 cut(s) 262
Bst2UI CCWGG 1 cut(s) 183
Bst4CI ACNGT 3 cut(s) 229, 275, 348
BstACI GRCGYC 1 cut(s) 262
BstBAI YACGTR 1 cut(s) 241
BstC8I GCNNGC 1 cut(s) 23
BstF5I GGATG 1 cut(s) 312
BstH2I RGCGCY 1 cut(s) 265
BstHHI GCGC 2 cut(s) 192, 264
BstKTI GATC 2 cut(s) 283, 287
BstMAI GTCTC 2 cut(s) 61, 79
BstMBI GATC 2 cut(s) 280, 284
BstMWI GCNNNNNNNGC 5 cut(s) 48, 51, 187, 203, 320
BstNI CCWGG 1 cut(s) 183
BstSCI CCNGG 1 cut(s) 181
BstSFI CTRYAG 1 cut(s) 324
BstSNI TACGTA 1 cut(s) 241
BstV1I GCAGC 1 cut(s) 38
Bsu15I ATCGAT 1 cut(s) 283
BsuRI GGCC 2 cut(s) 42, 74
BsuTUI ATCGAT 1 cut(s) 283
BtsCI GGATG 1 cut(s) 312
BtsIMutI CAGTG 3 cut(s) 53, 225, 308
Cac8I GCNNGC 1 cut(s) 23
CciI TCATGA 1 cut(s) 61
CfoI GCGC 2 cut(s) 192, 264
Cfr13I GGNCC 2 cut(s) 40, 72
ClaI ATCGAT 1 cut(s) 283
CviAII CATG 2 cut(s) 62, 152
CviJI RGCY 5 cut(s) 14, 42, 74, 181, 323
CviKI_1 RGCY 5 cut(s) 14, 42, 74, 181, 323
DinI GGCGCC 1 cut(s) 263
DpnI GATC 2 cut(s) 282, 286
DpnII GATC 2 cut(s) 280, 284
Eco105I TACGTA 1 cut(s) 241
Eco31I GGTCTC 1 cut(s) 79
EcoRII CCWGG 1 cut(s) 181
EgeI GGCGCC 1 cut(s) 263
EheI GGCGCC 1 cut(s) 263
Esp3I CGTCTC 1 cut(s) 61
FaeI CATG 2 cut(s) 65, 155
FaiI YATR 3 cut(s) 63, 153, 371
FaqI GGGAC 1 cut(s) 343
FatI CATG 2 cut(s) 61, 151
FauI CCCGC 1 cut(s) 28
Fnu4HI GCNGC 5 cut(s) 43, 52, 75, 214, 379
FokI GGATG 1 cut(s) 319
Fsp4HI GCNGC 5 cut(s) 43, 52, 75, 214, 379
FspBI CTAG 1 cut(s) 98
GlaI GCGC 2 cut(s) 191, 263
GluI GCNGC 5 cut(s) 43, 52, 75, 214, 379
HaeII RGCGCY 1 cut(s) 265
HaeIII GGCC 2 cut(s) 42, 74
HapII CCGG 1 cut(s) 210
HhaI GCGC 2 cut(s) 192, 264
Hin1I GRCGYC 1 cut(s) 262
Hin1II CATG 2 cut(s) 65, 155
Hin6I GCGC 2 cut(s) 190, 262
HinP1I GCGC 2 cut(s) 190, 262
HinfI GANTC 4 cut(s) 5, 145, 221, 334
HpaII CCGG 1 cut(s) 210
HphI GGTGA 1 cut(s) 197
Hpy188I TCNGA 1 cut(s) 250
Hpy188III TCNNGA 4 cut(s) 62, 82, 278, 340
Hpy99I CGWCG 1 cut(s) 20
HpyAV CCTTC 1 cut(s) 25
HpyCH4III ACNGT 3 cut(s) 229, 275, 348
HpyCH4IV ACGT 1 cut(s) 240
HpyCH4V TGCA 2 cut(s) 54, 111
HpyF10VI GCNNNNNNNGC 5 cut(s) 48, 51, 187, 203, 320
HpySE526I ACGT 1 cut(s) 240
Hsp92I GRCGYC 1 cut(s) 262
Hsp92II CATG 2 cut(s) 65, 155
HspAI GCGC 2 cut(s) 190, 262
KasI GGCGCC 1 cut(s) 261
Kzo9I GATC 2 cut(s) 280, 284
Lsp1109I GCAGC 1 cut(s) 38
LweI GCATC 1 cut(s) 63
MaeI CTAG 1 cut(s) 98
MaeII ACGT 1 cut(s) 240
MalI GATC 2 cut(s) 282, 286
MboI GATC 2 cut(s) 280, 284
MboII GAAGA 2 cut(s) 40, 279
MluCI AATT 2 cut(s) 251, 268
Mly113I GGCGCC 1 cut(s) 262
MlyI GAGTC 1 cut(s) 14
MnlI CCTC 8 cut(s) 89, 121, 130, 133, 209, 217, 242, 303
MseI TTAA 1 cut(s) 177
MspA1I CMGCKG 1 cut(s) 381
MspI CCGG 1 cut(s) 210
MspR9I CCNGG 1 cut(s) 183
MvaI CCWGG 1 cut(s) 183
MwoI GCNNNNNNNGC 5 cut(s) 48, 51, 187, 203, 320
NarI GGCGCC 1 cut(s) 262
NdeII GATC 2 cut(s) 280, 284
NlaIII CATG 2 cut(s) 65, 155
NlaIV GGNNCC 3 cut(s) 41, 205, 263
PagI TCATGA 1 cut(s) 61
PcsI WCGNNNNNNNCGW 1 cut(s) 246
PfeI GAWTC 3 cut(s) 145, 221, 334
PkrI GCNGC 5 cut(s) 44, 53, 76, 215, 380
PleI GAGTC 1 cut(s) 13
PluTI GGCGCC 1 cut(s) 265
PpsI GAGTC 1 cut(s) 13
Ppu21I YACGTR 1 cut(s) 241
Psp6I CCWGG 1 cut(s) 181
PspGI CCWGG 1 cut(s) 181
PspN4I GGNNCC 3 cut(s) 41, 205, 263
PspPI GGNCC 2 cut(s) 40, 72
SaqAI TTAA 1 cut(s) 177
SatI GCNGC 5 cut(s) 43, 52, 75, 214, 379
Sau3AI GATC 2 cut(s) 280, 284
Sau96I GGNCC 2 cut(s) 40, 72
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 1 cut(s) 183
SetI ASST 2 cut(s) 132, 243
SfaNI GCATC 1 cut(s) 63
SfcI CTRYAG 1 cut(s) 324
SfoI GGCGCC 1 cut(s) 263
SmlI CTYRAG 1 cut(s) 80
SmoI CTYRAG 1 cut(s) 80
SnaBI TACGTA 1 cut(s) 241
Sse9I AATT 2 cut(s) 251, 268
SsiI CCGC 5 cut(s) 35, 43, 75, 213, 379
SspDI GGCGCC 1 cut(s) 261
SspMI CTAG 1 cut(s) 98
StyD4I CCNGG 1 cut(s) 181
TaaI ACNGT 3 cut(s) 229, 275, 348
TaiI ACGT 1 cut(s) 243
TaqI TCGA 2 cut(s) 162, 283
TasI AATT 2 cut(s) 251, 268
TauI GCSGC 4 cut(s) 45, 77, 216, 381
TfiI GAWTC 3 cut(s) 145, 221, 334
Tru1I TTAA 1 cut(s) 177
Tru9I TTAA 1 cut(s) 177
TscAI CASTG 3 cut(s) 53, 232, 308
TseI GCWGC 1 cut(s) 51
TspDTI ATGAA 2 cut(s) 137, 374
TspRI CASTG 3 cut(s) 53, 232, 308
XspI CTAG 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.