Rh6CG452800

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0005079
Physical Location & Seq
Reverse (-)
62932934 .. 62935319
2386 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG452800.1

Sequence Viewer

Length: 1053 bp
ATGGAAGGCTCGGCCGCCGGCCAGCCCAAGAAGTCCGGCGCTCGCCAGTGCTGCATCTGTAACCAGAAAAGAGCTGCTCTCAAGAGGCCCAAAACCCTAGAACAGATTTGCAGGGAGTGTTTCTATGAGGTATTTGAGGAGGAGATACATCAGGTGATTGTTGAAAACCAGTTGTTTAAGCCCGGTGAACGCATTGCCATTGGTGCCTCTGGTGGAAAAGATTCCACTGTTCTTGCCTATGTATTATCGGAATTGAATAAGCGGCACAATTATGGGTTGGATCTCTTCCTGTTGTCTGTGGATGAGGGCATTACAGGCTACAGGGATGATTCTCTTGAAACCGTTAAAAGAAATGAAATGCAGTATGGATTGCCGTTAAAGATTGTGTCGTACAAGGATTTGTATGGGTGGACGATGGATGAAATAGTAAAGATGATTGGTCTCAAGAATAATTGCACATTTTGTGGTGTTTTTCGTCGTCAGGCCCTTGATCGCGGTGCTGCATTGTTGAAAGTAGACAAGCTTGCTACCGGACATAATGCCGATGATATAGCTGAAACAGTTCTATTGAACATATTACGTGGTGATATTGCAAGATTGAGTAGATGCACTTCAATTGTCACTGGTGAAGATGGACCAATTCCAAGATGCAAACCTTTTAAATACACCTATGAGAAGGAAATTAGGCTGGATTACTTCTCCACTGAATGCATTTATTCACCAAATGCTTATCGTGGTTTTGCTCGTGAGTTCATCAAAGATTTGGAAAGAATCAGACCTAGGGCCATACTTGACATAATCAAATCAGGGGAGAATTTCAGAATTTCTACTACTACAAAAATGCCGGAACAGGGGACATGTGAACGCTGTGGTTACATTTCAAGCCAGAAATGGTGTAAAGCTTGTGTATTGCTTGAGGGACTGAATAGGGGTTTGCCAAAGATGGGAATTGGTCGGAATCGAGGAATTAACAACAATCAGAAGAAGGATATGAAACAAAGTAATGGAACAAAAAGTATAGAGAGCAAACAATGTGGGACTTTGGACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04122 map04122
Pfam Domains
Protein Families
ATP_bind_3 zn-ribbon_14

Protein Analysis

350

Amino Acids

39.52

Weight (kDa)

8.92

Isoelectric Point (pI)

34.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP_bind_3 PF01171 65 - 221 4.6e-12 PP-loop domain
zn-ribbon_14 PF16503 285 - 315 1.6e-18 Zinc-ribbon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 203
AccI GTMKAC 1 cut(s) 516
AccII CGCG 1 cut(s) 495
AciI CCGC 3 cut(s) 15, 262, 495
AclWI GGATC 1 cut(s) 288
AcoI YGGCCR 2 cut(s) 12, 19
AcsI RAATTY 2 cut(s) 814, 822
AfaI GTAC 1 cut(s) 392
AfiI CCNNNNNNNGG 2 cut(s) 851, 944
AflIII ACRYGT 1 cut(s) 857
AgsI TTSAA 7 cut(s) 164, 256, 338, 511, 571, 615, 882
AluBI AGCT 4 cut(s) 74, 523, 554, 902
AluI AGCT 4 cut(s) 74, 523, 554, 902
Alw26I GTCTC 1 cut(s) 446
AlwI GGATC 1 cut(s) 288
AoxI GGCC 5 cut(s) 12, 19, 86, 483, 783
ApeKI GCWGC 3 cut(s) 51, 74, 500
ApoI RAATTY 2 cut(s) 814, 822
Asp700I GAANNNNTTC 2 cut(s) 220, 561
AspA2I CCTAGG 1 cut(s) 779
AspLEI GCGC 1 cut(s) 41
AspS9I GGNCC 4 cut(s) 87, 484, 635, 783
AsuC2I CCSGG 1 cut(s) 183
AsuHPI GGTGA 5 cut(s) 166, 197, 596, 638, 711
AvaII GGWCC 1 cut(s) 635
AvrII CCTAGG 1 cut(s) 779
BanI GGYRCC 1 cut(s) 203
BarI GAAGNNNNNNTAC 2 cut(s) 595, 627
BauI CACGAG 1 cut(s) 744
BbvI GCAGC 3 cut(s) 38, 61, 487
BccI CCATC 3 cut(s) 409, 626, 937
BceAI ACGGC 1 cut(s) 358
BcnI CCSGG 1 cut(s) 183
BcoDI GTCTC 1 cut(s) 446
BfaI CTAG 2 cut(s) 98, 780
BfmI CTRYAG 1 cut(s) 319
BfoI RGCGCY 1 cut(s) 42
BisI GCNGC 5 cut(s) 15, 52, 75, 263, 501
BlnI CCTAGG 1 cut(s) 779
BlsI GCNGC 5 cut(s) 16, 53, 76, 264, 502
Bme1390I CCNGG 1 cut(s) 183
Bme18I GGWCC 1 cut(s) 635
BmgT120I GGNCC 4 cut(s) 87, 484, 635, 783
BmiI GGNNCC 1 cut(s) 205
BmrFI CCNGG 1 cut(s) 183
BmsI GCATC 3 cut(s) 63, 596, 638
BplI GAGNNNNNCTC 2 cut(s) 63, 95
BpuEI CTTGAG 3 cut(s) 65, 428, 935
BpuMI CCSGG 1 cut(s) 183
BsaAI YACGTR 1 cut(s) 581
BsaI GGTCTC 1 cut(s) 446
BsaJI CCNNGG 1 cut(s) 779
BsaWI WCCGGW 1 cut(s) 530
Bsc4I CCNNNNNNNGG 2 cut(s) 851, 944
Bse118I RCCGGY 1 cut(s) 17
Bse1I ACTGG 3 cut(s) 46, 169, 628
Bse3DI GCAATG 1 cut(s) 192
BseDI CCNNGG 1 cut(s) 779
BseGI GGATG 3 cut(s) 307, 331, 424
BseLI CCNNNNNNNGG 2 cut(s) 851, 944
BseMI GCAATG 1 cut(s) 192
BseNI ACTGG 3 cut(s) 46, 169, 628
BseRI GAGGAG 2 cut(s) 152, 155
BseX3I CGGCCG 1 cut(s) 12
BseXI GCAGC 3 cut(s) 38, 61, 487
Bsh1236I CGCG 1 cut(s) 495
Bsh1285I CGRYCG 1 cut(s) 15
BshFI GGCC 5 cut(s) 14, 21, 88, 485, 785
BshNI GGYRCC 1 cut(s) 203
BsiEI CGRYCG 1 cut(s) 15
BsiSI CCGG 5 cut(s) 18, 36, 183, 531, 845
BslFI GGGAC 2 cut(s) 868, 933
BslI CCNNNNNNNGG 2 cut(s) 851, 944
BsmAI GTCTC 1 cut(s) 446
BsmFI GGGAC 2 cut(s) 868, 933
BsmI GAATGC 1 cut(s) 713
BsnI GGCC 5 cut(s) 14, 21, 88, 485, 785
Bso31I GGTCTC 1 cut(s) 446
Bsp143I GATC 2 cut(s) 280, 490
BspACI CCGC 3 cut(s) 15, 262, 495
BspANI GGCC 5 cut(s) 14, 21, 88, 485, 785
BspFNI CGCG 1 cut(s) 495
BspLI GGNNCC 1 cut(s) 205
BspPI GGATC 1 cut(s) 288
BspT107I GGYRCC 1 cut(s) 203
BspTNI GGTCTC 1 cut(s) 446
BsrDI GCAATG 1 cut(s) 192
BsrFI RCCGGY 1 cut(s) 17
BsrI ACTGG 3 cut(s) 46, 169, 628
BssAI RCCGGY 1 cut(s) 17
BssECI CCNNGG 1 cut(s) 779
BssMI GATC 2 cut(s) 280, 490
BssSI CACGAG 1 cut(s) 744
BssT1I CCWWGG 1 cut(s) 779
Bst2BI CACGAG 1 cut(s) 744
Bst4CI ACNGT 3 cut(s) 229, 343, 562
Bst6I CTCTTC 1 cut(s) 290
BstBAI YACGTR 1 cut(s) 581
BstC8I GCNNGC 4 cut(s) 19, 23, 43, 525
BstF5I GGATG 3 cut(s) 307, 331, 424
BstFNI CGCG 1 cut(s) 495
BstH2I RGCGCY 1 cut(s) 42
BstHHI GCGC 1 cut(s) 41
BstKTI GATC 2 cut(s) 283, 493
BstMAI GTCTC 1 cut(s) 446
BstMBI GATC 2 cut(s) 280, 490
BstMCI CGRYCG 1 cut(s) 15
BstMWI GCNNNNNNNGC 3 cut(s) 51, 203, 315
BstNSI RCATGY 1 cut(s) 861
BstSCI CCNGG 1 cut(s) 181
BstSFI CTRYAG 1 cut(s) 319
BstUI CGCG 1 cut(s) 495
BstV1I GCAGC 3 cut(s) 38, 61, 487
BstX2I RGATCY 1 cut(s) 280
BstYI RGATCY 1 cut(s) 280
BstZI CGGCCG 1 cut(s) 12
BsuRI GGCC 5 cut(s) 14, 21, 88, 485, 785
BtsCI GGATG 3 cut(s) 307, 331, 424
BtsIMutI CAGTG 4 cut(s) 53, 225, 621, 702
Cac8I GCNNGC 4 cut(s) 19, 23, 43, 525
CfoI GCGC 1 cut(s) 41
Cfr10I RCCGGY 1 cut(s) 17
Cfr13I GGNCC 4 cut(s) 87, 484, 635, 783
Csp6I GTAC 1 cut(s) 391
CspCI CAANNNNNGTGG 4 cut(s) 214, 249, 1015, 1050
CviAII CATG 1 cut(s) 858
CviQI GTAC 1 cut(s) 391
DpnI GATC 2 cut(s) 282, 492
DpnII GATC 2 cut(s) 280, 490
DraI TTTAAA 1 cut(s) 661
EaeI YGGCCR 2 cut(s) 12, 19
EagI CGGCCG 1 cut(s) 12
Eam1104I CTCTTC 1 cut(s) 290
EarI CTCTTC 1 cut(s) 290
EclXI CGGCCG 1 cut(s) 12
Eco130I CCWWGG 1 cut(s) 779
Eco31I GGTCTC 1 cut(s) 446
Eco47I GGWCC 1 cut(s) 635
Eco52I CGGCCG 1 cut(s) 12
EcoO109I RGGNCCY 1 cut(s) 484
EcoT14I CCWWGG 1 cut(s) 779
EcoT22I ATGCAT 1 cut(s) 713
ErhI CCWWGG 1 cut(s) 779
FaeI CATG 1 cut(s) 861
FaqI GGGAC 2 cut(s) 868, 933
FatI CATG 1 cut(s) 857
FblI GTMKAC 1 cut(s) 516
Fnu4HI GCNGC 5 cut(s) 15, 52, 75, 263, 501
FokI GGATG 3 cut(s) 314, 338, 431
Fsp4HI GCNGC 5 cut(s) 15, 52, 75, 263, 501
FspBI CTAG 2 cut(s) 98, 780
GlaI GCGC 1 cut(s) 40
GluI GCNGC 5 cut(s) 15, 52, 75, 263, 501
HaeII RGCGCY 1 cut(s) 42
HaeIII GGCC 5 cut(s) 14, 21, 88, 485, 785
HapII CCGG 5 cut(s) 18, 36, 183, 531, 845
HhaI GCGC 1 cut(s) 41
Hin1II CATG 1 cut(s) 861
Hin6I GCGC 1 cut(s) 39
HinP1I GCGC 1 cut(s) 39
HindIII AAGCTT 2 cut(s) 521, 900
HinfI GANTC 4 cut(s) 221, 329, 771, 958
HpaII CCGG 5 cut(s) 18, 36, 183, 531, 845
HphI GGTGA 5 cut(s) 166, 197, 596, 638, 711
Hpy166II GTNNAC 4 cut(s) 188, 411, 517, 863
Hpy188I TCNGA 6 cut(s) 250, 776, 821, 957, 981, 1052
Hpy188III TCNNGA 4 cut(s) 82, 335, 445, 746
Hpy8I GTNNAC 4 cut(s) 188, 411, 517, 863
Hpy99I CGWCG 1 cut(s) 480
HpyAV CCTTC 2 cut(s) 670, 979
HpyCH4III ACNGT 3 cut(s) 229, 343, 562
HpyCH4IV ACGT 1 cut(s) 580
HpyCH4V TGCA 9 cut(s) 54, 111, 361, 456, 503, 593, 609, 651, 711
HpyF10VI GCNNNNNNNGC 3 cut(s) 51, 203, 315
HpySE526I ACGT 1 cut(s) 580
Hsp92II CATG 1 cut(s) 861
HspAI GCGC 1 cut(s) 39
KroI GCCGGC 1 cut(s) 17
KroNI GCCGGC 1 cut(s) 19
Kzo9I GATC 2 cut(s) 280, 490
Lsp1109I GCAGC 3 cut(s) 38, 61, 487
LweI GCATC 3 cut(s) 63, 596, 638
MaeI CTAG 2 cut(s) 98, 780
MaeII ACGT 1 cut(s) 580
MaeIII GTNAC 3 cut(s) 59, 619, 872
MalI GATC 2 cut(s) 282, 492
MboI GATC 2 cut(s) 280, 490
MboII GAAGA 3 cut(s) 277, 641, 994
MfeI CAATTG 1 cut(s) 615
MflI RGATCY 1 cut(s) 280
MmeI TCCRAC 2 cut(s) 258, 935
MnlI CCTC 8 cut(s) 78, 121, 130, 133, 217, 298, 910, 956
Mph1103I ATGCAT 1 cut(s) 713
MroNI GCCGGC 1 cut(s) 17
MroXI GAANNNNTTC 2 cut(s) 220, 561
MseI TTAA 5 cut(s) 177, 345, 377, 660, 969
MslI CAYNNNNRTG 1 cut(s) 270
MspI CCGG 5 cut(s) 18, 36, 183, 531, 845
MspR9I CCNGG 1 cut(s) 183
MunI CAATTG 1 cut(s) 615
Mva1269I GAATGC 1 cut(s) 713
MvnI CGCG 1 cut(s) 495
MwoI GCNNNNNNNGC 3 cut(s) 51, 203, 315
NaeI GCCGGC 1 cut(s) 19
NciI CCSGG 1 cut(s) 183
NdeII GATC 2 cut(s) 280, 490
NgoMIV GCCGGC 1 cut(s) 17
NlaIII CATG 1 cut(s) 861
NlaIV GGNNCC 1 cut(s) 205
NmuCI GTSAC 1 cut(s) 619
NsiI ATGCAT 1 cut(s) 713
NspI RCATGY 1 cut(s) 861
PciI ACATGT 1 cut(s) 857
PctI GAATGC 1 cut(s) 713
PdiI GCCGGC 1 cut(s) 19
PdmI GAANNNNTTC 2 cut(s) 220, 561
PfeI GAWTC 4 cut(s) 221, 329, 771, 958
PkrI GCNGC 5 cut(s) 16, 53, 76, 264, 502
Ppu21I YACGTR 1 cut(s) 581
PscI ACATGT 1 cut(s) 857
PspN4I GGNNCC 1 cut(s) 205
PspPI GGNCC 4 cut(s) 87, 484, 635, 783
PsuI RGATCY 1 cut(s) 280
RsaI GTAC 1 cut(s) 392
RsaNI GTAC 1 cut(s) 391
RseI CAYNNNNRTG 1 cut(s) 270
SaqAI TTAA 5 cut(s) 177, 345, 377, 660, 969
SatI GCNGC 5 cut(s) 15, 52, 75, 263, 501
Sau3AI GATC 2 cut(s) 280, 490
Sau96I GGNCC 4 cut(s) 87, 484, 635, 783
ScrFI CCNGG 1 cut(s) 183
SfaNI GCATC 3 cut(s) 63, 596, 638
SfcI CTRYAG 1 cut(s) 319
SinI GGWCC 1 cut(s) 635
SmiMI CAYNNNNRTG 1 cut(s) 270
SmlI CTYRAG 3 cut(s) 80, 443, 914
SmoI CTYRAG 3 cut(s) 80, 443, 914
SsiI CCGC 3 cut(s) 15, 262, 495
SspMI CTAG 2 cut(s) 98, 780
StyD4I CCNGG 1 cut(s) 181
StyI CCWWGG 1 cut(s) 779
TaaI ACNGT 3 cut(s) 229, 343, 562
TaiI ACGT 1 cut(s) 583
TaqI TCGA 1 cut(s) 961
TauI GCSGC 2 cut(s) 17, 265
TfiI GAWTC 4 cut(s) 221, 329, 771, 958
Tru1I TTAA 5 cut(s) 177, 345, 377, 660, 969
Tru9I TTAA 5 cut(s) 177, 345, 377, 660, 969
TscAI CASTG 4 cut(s) 53, 232, 628, 709
TseFI GTSAC 1 cut(s) 619
TseI GCWGC 3 cut(s) 51, 74, 500
Tsp45I GTSAC 1 cut(s) 619
TspDTI ATGAA 4 cut(s) 369, 435, 742, 1007
TspRI CASTG 4 cut(s) 53, 232, 628, 709
VpaK11BI GGWCC 1 cut(s) 635
XapI RAATTY 2 cut(s) 814, 822
XceI RCATGY 1 cut(s) 861
XmaJI CCTAGG 1 cut(s) 779
XmiI GTMKAC 1 cut(s) 516
XmnI GAANNNNTTC 2 cut(s) 220, 561
XspI CTAG 2 cut(s) 98, 780
Zsp2I ATGCAT 1 cut(s) 713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.