MD15G1004300.v1.1

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
Physical Location & Seq
Forward (+)
257717 .. 260249
2533 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1004300.v1.1.491

Sequence Viewer

Length: 966 bp
ATGGAGTCGTCAGCCGACGGCAAGCCGAAGAAGGCGGGGGGCCGCCAGTGCTGCATCTGTCATGAGAGACGGGCCGCCCTCAAGAGACCCAAAACCCTAGAGCAGATTTGCAGGGAATGTTTCTACGAGGTGTTTGAGGAGGAGATTCATCAGGTCATCGTCGAAAACCAGTTATTTAAGCCTGGTGAGCGCATTGCCCTCGGTGCCTCCGGCGGCAAACGTAAATTAATTAATCAAGCCGTGCTTCCAGTGCTAGTGAATTCCACTGTCCTCGCTTATGTATTATCCGAATTGAATAGGCGGCACAATTACGGTTTGGATCTCTTCCTCTTGTCAGTCGATGAGGGCATTACTGGCTACAGGGACGATTCTCTTGAAACCGTCAAAAGAAATGAAATCCAGTATGGATTGCCGCTGAAAATAGTCTCCTACAAGGATTTGTATGGGTGGACCATGGATGAAATAGTAAAGATGATAGGTCTCAAGAACAATTGCACCTTCTGTGGTGTTTTTTGTCGTCAGGCCCTTGATCGTGGTGCTGCACTGTTGAAGGTAGACAAGCTAGCTACTGGACATAATGCAGATGATATTGCTGAAACAGTTCTTTTGAACTTATTAAGAGGCATTTATTCACCCAATGCATATCGTGGTTTTGCTCGTGAATTCATCAAGGATTTAGAAAGAATCAGACCTAGGGCTATACTTGACCTCATCAAATCAGGGGAAAATTTCAGAATTTCAACTTGTACAAAAATGCCAGAACAGGGGACATGTGAACGGTGCGGTTACATTTCCAGCCAGAAATGGTGCAAGGCTTGTGTTTTGCTAGAGGGGCTGAATAAGGGTTTGCCAAAGCTGGGAATTGGCCGGAGTCGAGGACTTAATAACGATGGGAAGAATGATACGAAGGAAAGAAATGGAACAAAAAGTATTGAAAGCAAGCAATGTGGAAGTTTGGATTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
Pfam Domains
Protein Families

Protein Analysis

322

Amino Acids

36.01

Weight (kDa)

8.91

Isoelectric Point (pI)

35.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zn-ribbon_14 PF16503 256 - 286 1.2e-18 Zinc-ribbon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 203
AccI GTMKAC 1 cut(s) 555
AciI CCGC 7 cut(s) 35, 43, 75, 213, 301, 413, 783
AclWI GGATC 1 cut(s) 327
AcoI YGGCCR 1 cut(s) 865
AcsI RAATTY 4 cut(s) 259, 662, 727, 735
AfaI GTAC 1 cut(s) 748
AfiI CCNNNNNNNGG 2 cut(s) 764, 857
AflIII ACRYGT 1 cut(s) 770
AgsI TTSAA 6 cut(s) 295, 377, 550, 610, 741, 935
AjnI CCWGG 1 cut(s) 181
AluBI AGCT 3 cut(s) 562, 566, 856
AluI AGCT 3 cut(s) 562, 566, 856
Alw26I GTCTC 4 cut(s) 61, 79, 430, 485
AlwI GGATC 1 cut(s) 327
AoxI GGCC 4 cut(s) 40, 72, 522, 865
ApeKI GCWGC 2 cut(s) 51, 539
ApoI RAATTY 4 cut(s) 259, 662, 727, 735
AseI ATTAAT 2 cut(s) 227, 231
Asp700I GAANNNNTTC 1 cut(s) 600
AspA2I CCTAGG 1 cut(s) 692
AspLEI GCGC 1 cut(s) 192
AspS9I GGNCC 4 cut(s) 40, 72, 450, 523
AsuHPI GGTGA 2 cut(s) 197, 624
AsuNHI GCTAGC 1 cut(s) 562
AvaII GGWCC 1 cut(s) 450
AvrII CCTAGG 1 cut(s) 692
BanI GGYRCC 1 cut(s) 203
BauI CACGAG 1 cut(s) 657
BbvI GCAGC 2 cut(s) 38, 526
BccI CCATC 1 cut(s) 884
BceAI ACGGC 2 cut(s) 34, 224
BcgI CGANNNNNNTGC 2 cut(s) 181, 215
BciT130I CCWGG 1 cut(s) 183
BcoDI GTCTC 4 cut(s) 61, 79, 430, 485
BfaI CTAG 5 cut(s) 98, 254, 563, 693, 827
BfmI CTRYAG 1 cut(s) 358
BisI GCNGC 7 cut(s) 43, 52, 75, 214, 302, 413, 540
BlnI CCTAGG 1 cut(s) 692
BlsI GCNGC 7 cut(s) 44, 53, 76, 215, 303, 414, 541
Bme1390I CCNGG 1 cut(s) 183
Bme18I GGWCC 1 cut(s) 450
BmgT120I GGNCC 4 cut(s) 40, 72, 450, 523
BmiI GGNNCC 2 cut(s) 41, 205
BmrFI CCNGG 1 cut(s) 183
BmsI GCATC 1 cut(s) 63
BmtI GCTAGC 1 cut(s) 566
BpuEI CTTGAG 2 cut(s) 65, 467
BsaI GGTCTC 2 cut(s) 79, 485
BsaJI CCNNGG 3 cut(s) 199, 453, 692
Bsc4I CCNNNNNNNGG 2 cut(s) 764, 857
Bse1I ACTGG 6 cut(s) 46, 169, 248, 358, 400, 574
Bse3DI GCAATG 2 cut(s) 192, 950
BseBI CCWGG 1 cut(s) 183
BseDI CCNNGG 3 cut(s) 199, 453, 692
BseGI GGATG 1 cut(s) 463
BseLI CCNNNNNNNGG 2 cut(s) 764, 857
BseMI GCAATG 2 cut(s) 192, 950
BseNI ACTGG 6 cut(s) 46, 169, 248, 358, 400, 574
BseRI GAGGAG 2 cut(s) 152, 155
BseXI GCAGC 2 cut(s) 38, 526
BseYI CCCAGC 1 cut(s) 856
BsgI GTGCAG 1 cut(s) 525
BshFI GGCC 4 cut(s) 42, 74, 524, 867
BshNI GGYRCC 1 cut(s) 203
BsiSI CCGG 2 cut(s) 210, 868
BslFI GGGAC 2 cut(s) 377, 781
BslI CCNNNNNNNGG 2 cut(s) 764, 857
BsmAI GTCTC 4 cut(s) 61, 79, 430, 485
BsmBI CGTCTC 1 cut(s) 61
BsmFI GGGAC 2 cut(s) 377, 781
BsnI GGCC 4 cut(s) 42, 74, 524, 867
Bso31I GGTCTC 2 cut(s) 79, 485
Bsp1407I TGTACA 1 cut(s) 746
Bsp143I GATC 2 cut(s) 319, 529
Bsp19I CCATGG 1 cut(s) 453
BspACI CCGC 7 cut(s) 35, 43, 75, 213, 301, 413, 783
BspANI GGCC 4 cut(s) 42, 74, 524, 867
BspHI TCATGA 1 cut(s) 61
BspLI GGNNCC 2 cut(s) 41, 205
BspOI GCTAGC 1 cut(s) 566
BspPI GGATC 1 cut(s) 327
BspT107I GGYRCC 1 cut(s) 203
BspTNI GGTCTC 2 cut(s) 79, 485
BsrDI GCAATG 2 cut(s) 192, 950
BsrGI TGTACA 1 cut(s) 746
BsrI ACTGG 6 cut(s) 46, 169, 248, 358, 400, 574
BssECI CCNNGG 3 cut(s) 199, 453, 692
BssMI GATC 2 cut(s) 319, 529
BssSI CACGAG 1 cut(s) 657
BssT1I CCWWGG 2 cut(s) 453, 692
Bst2BI CACGAG 1 cut(s) 657
Bst2UI CCWGG 1 cut(s) 183
Bst4CI ACNGT 6 cut(s) 268, 314, 382, 546, 601, 780
Bst6I CTCTTC 1 cut(s) 329
BstAUI TGTACA 1 cut(s) 746
BstC8I GCNNGC 3 cut(s) 23, 564, 941
BstDSI CCRYGG 1 cut(s) 453
BstF5I GGATG 1 cut(s) 463
BstHHI GCGC 1 cut(s) 192
BstKTI GATC 2 cut(s) 322, 532
BstMAI GTCTC 4 cut(s) 61, 79, 430, 485
BstMBI GATC 2 cut(s) 319, 529
BstMWI GCNNNNNNNGC 7 cut(s) 48, 51, 187, 203, 250, 354, 832
BstNI CCWGG 1 cut(s) 183
BstNSI RCATGY 1 cut(s) 774
BstSCI CCNGG 1 cut(s) 181
BstSFI CTRYAG 1 cut(s) 358
BstV1I GCAGC 2 cut(s) 38, 526
BstX2I RGATCY 1 cut(s) 319
BstYI RGATCY 1 cut(s) 319
BsuRI GGCC 4 cut(s) 42, 74, 524, 867
BtgI CCRYGG 1 cut(s) 453
BtsCI GGATG 1 cut(s) 463
BtsIMutI CAGTG 4 cut(s) 53, 255, 264, 542
Cac8I GCNNGC 3 cut(s) 23, 564, 941
CciI TCATGA 1 cut(s) 61
CfoI GCGC 1 cut(s) 192
Cfr13I GGNCC 4 cut(s) 40, 72, 450, 523
Csp6I GTAC 1 cut(s) 747
CspCI CAANNNNNGTGG 2 cut(s) 928, 963
CviAII CATG 3 cut(s) 62, 454, 771
CviQI GTAC 1 cut(s) 747
DpnI GATC 2 cut(s) 321, 531
DpnII GATC 2 cut(s) 319, 529
EaeI YGGCCR 1 cut(s) 865
Eam1104I CTCTTC 1 cut(s) 329
EarI CTCTTC 1 cut(s) 329
Eco130I CCWWGG 2 cut(s) 453, 692
Eco31I GGTCTC 2 cut(s) 79, 485
Eco47I GGWCC 1 cut(s) 450
EcoO109I RGGNCCY 1 cut(s) 523
EcoRI GAATTC 2 cut(s) 259, 662
EcoRII CCWGG 1 cut(s) 181
EcoT14I CCWWGG 2 cut(s) 453, 692
EcoT22I ATGCAT 1 cut(s) 643
ErhI CCWWGG 2 cut(s) 453, 692
Esp3I CGTCTC 1 cut(s) 61
FaeI CATG 3 cut(s) 65, 457, 774
FaiI YATR 9 cut(s) 63, 279, 405, 444, 455, 576, 643, 701, 772
FalI AAGNNNNNCTT 2 cut(s) 228, 260
FaqI GGGAC 2 cut(s) 377, 781
FatI CATG 3 cut(s) 61, 453, 770
FauI CCCGC 1 cut(s) 28
FblI GTMKAC 1 cut(s) 555
Fnu4HI GCNGC 7 cut(s) 43, 52, 75, 214, 302, 413, 540
FokI GGATG 1 cut(s) 470
Fsp4HI GCNGC 7 cut(s) 43, 52, 75, 214, 302, 413, 540
FspBI CTAG 5 cut(s) 98, 254, 563, 693, 827
GlaI GCGC 1 cut(s) 191
GluI GCNGC 7 cut(s) 43, 52, 75, 214, 302, 413, 540
GsaI CCCAGC 1 cut(s) 860
HaeIII GGCC 4 cut(s) 42, 74, 524, 867
HapII CCGG 2 cut(s) 210, 868
HhaI GCGC 1 cut(s) 192
Hin1II CATG 3 cut(s) 65, 457, 774
Hin6I GCGC 1 cut(s) 190
HinP1I GCGC 1 cut(s) 190
HinfI GANTC 5 cut(s) 5, 145, 368, 684, 871
HpaII CCGG 2 cut(s) 210, 868
HphI GGTGA 2 cut(s) 197, 624
Hpy166II GTNNAC 3 cut(s) 450, 556, 776
Hpy188I TCNGA 4 cut(s) 289, 689, 734, 965
Hpy188III TCNNGA 5 cut(s) 62, 82, 374, 484, 659
Hpy8I GTNNAC 3 cut(s) 450, 556, 776
Hpy99I CGWCG 2 cut(s) 20, 164
HpyAV CCTTC 4 cut(s) 25, 508, 544, 901
HpyCH4III ACNGT 6 cut(s) 268, 314, 382, 546, 601, 780
HpyCH4IV ACGT 1 cut(s) 220
HpyCH4V TGCA 7 cut(s) 54, 111, 495, 542, 581, 641, 810
HpyF10VI GCNNNNNNNGC 7 cut(s) 48, 51, 187, 203, 250, 354, 832
HpySE526I ACGT 1 cut(s) 220
Hsp92II CATG 3 cut(s) 65, 457, 774
HspAI GCGC 1 cut(s) 190
Kzo9I GATC 2 cut(s) 319, 529
Lsp1109I GCAGC 2 cut(s) 38, 526
LweI GCATC 1 cut(s) 63
MaeI CTAG 5 cut(s) 98, 254, 563, 693, 827
MaeII ACGT 1 cut(s) 220
MaeIII GTNAC 1 cut(s) 785
MalI GATC 2 cut(s) 321, 531
MboI GATC 2 cut(s) 319, 529
MboII GAAGA 3 cut(s) 40, 316, 907
MfeI CAATTG 1 cut(s) 490
MflI RGATCY 1 cut(s) 319
MlyI GAGTC 2 cut(s) 14, 880
Mph1103I ATGCAT 1 cut(s) 643
MroXI GAANNNNTTC 1 cut(s) 600
MseI TTAA 5 cut(s) 177, 227, 231, 617, 882
MspA1I CMGCKG 1 cut(s) 415
MspI CCGG 2 cut(s) 210, 868
MspR9I CCNGG 1 cut(s) 183
MunI CAATTG 1 cut(s) 490
MvaI CCWGG 1 cut(s) 183
MwoI GCNNNNNNNGC 7 cut(s) 48, 51, 187, 203, 250, 354, 832
NcoI CCATGG 1 cut(s) 453
NdeII GATC 2 cut(s) 319, 529
NheI GCTAGC 1 cut(s) 562
NlaIII CATG 3 cut(s) 65, 457, 774
NlaIV GGNNCC 2 cut(s) 41, 205
NsiI ATGCAT 1 cut(s) 643
NspI RCATGY 1 cut(s) 774
PacI TTAATTAA 1 cut(s) 231
PagI TCATGA 1 cut(s) 61
PciI ACATGT 1 cut(s) 770
PdmI GAANNNNTTC 1 cut(s) 600
PfeI GAWTC 3 cut(s) 145, 368, 684
PkrI GCNGC 7 cut(s) 44, 53, 76, 215, 303, 414, 541
PleI GAGTC 2 cut(s) 13, 879
PpsI GAGTC 2 cut(s) 13, 879
PscI ACATGT 1 cut(s) 770
PshBI ATTAAT 2 cut(s) 227, 231
Psp6I CCWGG 1 cut(s) 181
PspFI CCCAGC 1 cut(s) 856
PspGI CCWGG 1 cut(s) 181
PspN4I GGNNCC 2 cut(s) 41, 205
PspPI GGNCC 4 cut(s) 40, 72, 450, 523
PsuI RGATCY 1 cut(s) 319
RsaI GTAC 1 cut(s) 748
RsaNI GTAC 1 cut(s) 747
SaqAI TTAA 5 cut(s) 177, 227, 231, 617, 882
SatI GCNGC 7 cut(s) 43, 52, 75, 214, 302, 413, 540
Sau3AI GATC 2 cut(s) 319, 529
Sau96I GGNCC 4 cut(s) 40, 72, 450, 523
SchI GAGTC 2 cut(s) 14, 880
ScrFI CCNGG 1 cut(s) 183
SfaNI GCATC 1 cut(s) 63
SfcI CTRYAG 1 cut(s) 358
SinI GGWCC 1 cut(s) 450
SmlI CTYRAG 2 cut(s) 80, 482
SmoI CTYRAG 2 cut(s) 80, 482
SsiI CCGC 7 cut(s) 35, 43, 75, 213, 301, 413, 783
SspMI CTAG 5 cut(s) 98, 254, 563, 693, 827
StyD4I CCNGG 1 cut(s) 181
StyI CCWWGG 2 cut(s) 453, 692
TaaI ACNGT 6 cut(s) 268, 314, 382, 546, 601, 780
TaiI ACGT 1 cut(s) 223
TaqI TCGA 3 cut(s) 162, 339, 874
TatI WGTACW 1 cut(s) 746
TauI GCSGC 5 cut(s) 45, 77, 216, 304, 415
TfiI GAWTC 3 cut(s) 145, 368, 684
Tru1I TTAA 5 cut(s) 177, 227, 231, 617, 882
Tru9I TTAA 5 cut(s) 177, 227, 231, 617, 882
TscAI CASTG 4 cut(s) 53, 255, 271, 549
TseI GCWGC 2 cut(s) 51, 539
TspDTI ATGAA 4 cut(s) 137, 408, 474, 655
TspRI CASTG 4 cut(s) 53, 255, 271, 549
VpaK11BI GGWCC 1 cut(s) 450
VspI ATTAAT 2 cut(s) 227, 231
XapI RAATTY 4 cut(s) 259, 662, 727, 735
XceI RCATGY 1 cut(s) 774
XmaJI CCTAGG 1 cut(s) 692
XmiI GTMKAC 1 cut(s) 555
XmnI GAANNNNTTC 1 cut(s) 600
XspI CTAG 5 cut(s) 98, 254, 563, 693, 827
Zsp2I ATGCAT 1 cut(s) 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.