MD08G1004800.v1.1

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0005079
Physical Location & Seq
Forward (+)
442149 .. 445054
2906 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1004800.v1.1.491

Sequence Viewer

Length: 1053 bp
ATGGAGTCATCAGCCGACGGCAAGCAGAAGAAGGCGGGGGGCCGCCAGTGCTGCATCTGTCATGAGAGACGGGCCGCCCTCAAGAGACCCAAAACCCTAGAGCAGATTTGCAGGGAGTGTTTCTACAAGGTGTTTGAGGAGGAGATTCATCAGGTCATCGTCGAAAACCAGTTATTTAAGCCTGGTGAGCGCATTGCCCTCGGTGCCTCCGGCGGCAAAGATTCCACTGTCCTCGCTTACGTATTATCCGAATTGAATAGGCGGCACAATTACGGTCTGGATCTCTTCCTCTTGTCAGTGGATGAGGGCATTACTGGCTACAGGGACGATTCTCTTGAAACCGTCAAAAGAAATGAAATCCAGTATGGTTTGCCGCTGAAAATAGTCTCGTACAAGGATTTGTATGGGTGGACCATGGATGAAATAGTAAAGATGATTGGTCTCAAGAACAATTGCACCTTCTGTGGTGTTTTTCGTCGTCAGGCCCTTGATCGTGGCGCTGCACTGTTGAAGGTAGACAAGCTTGCTACTGGACATAATGCAGATGATATTGCTGAAACAGTTCTATTGAACATTTTACGAGGTGACATTGCAAGATTAAGTAGATGCACTTCAATTGTCACTGGTGAAGACGGGCCAATTCCAAGATGCAAACCTTTTAAATACACCTATGAGAAGGAGATTAGGCTGGACTACTTCTCCACTGAATGCATTTATTCACCCAATGCGTATCGTGGTTTTGCTCGTGAGTTCATCAAGGATTTAGAAAGAATCAGACCCAGGGCAATACTTGACATCATCAAATCAGGGGAGAATTTCAGAATTTCAACTTCAACAAAGATGCCAGAACAGGGGACATGTGAACGGTGTGGTTACATTTCCAGTCAGAAATGGTGCAAGGCCTGTGTTTTGCTTGAGGGGCTGAATAAGGGTTTGCCAAAGCTGGGAATTGGCCGGACTCGAGGACTTAATAACGATGACAAGAAGGATGCTAAGGAAAGAAATGGAACAAAAAGTATCGAAAGCAAGCAATGTGGAAGTTTGGATTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04122 map04122
Pfam Domains
Protein Families
ATP_bind_3 zn-ribbon_14

Protein Analysis

351

Amino Acids

39.57

Weight (kDa)

8.85

Isoelectric Point (pI)

36.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP_bind_3 PF01171 65 - 221 2.2e-11 PP-loop domain
zn-ribbon_14 PF16503 285 - 315 1.3e-18 Zinc-ribbon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 203
AccI GTMKAC 1 cut(s) 516
AciI CCGC 6 cut(s) 35, 43, 75, 213, 262, 374
AclWI GGATC 1 cut(s) 288
AcoI YGGCCR 1 cut(s) 952
AcsI RAATTY 2 cut(s) 814, 822
AfaI GTAC 1 cut(s) 392
AfiI CCNNNNNNNGG 2 cut(s) 851, 944
AflIII ACRYGT 1 cut(s) 857
AgsI TTSAA 7 cut(s) 256, 338, 511, 571, 615, 828, 834
AjnI CCWGG 2 cut(s) 181, 779
AluBI AGCT 2 cut(s) 523, 943
AluI AGCT 2 cut(s) 523, 943
Alw26I GTCTC 4 cut(s) 61, 79, 391, 446
AlwI GGATC 1 cut(s) 288
Ama87I CYCGRG 1 cut(s) 960
AoxI GGCC 6 cut(s) 40, 72, 483, 635, 900, 952
ApeKI GCWGC 2 cut(s) 51, 500
ApoI RAATTY 2 cut(s) 814, 822
Asp700I GAANNNNTTC 1 cut(s) 561
AspLEI GCGC 2 cut(s) 192, 500
AspS9I GGNCC 5 cut(s) 40, 72, 411, 484, 635
AsuHPI GGTGA 4 cut(s) 197, 596, 638, 711
AvaI CYCGRG 1 cut(s) 960
AvaII GGWCC 1 cut(s) 411
BanI GGYRCC 1 cut(s) 203
BarI GAAGNNNNNNTAC 2 cut(s) 595, 627
BauI CACGAG 1 cut(s) 744
BbsI GAAGAC 1 cut(s) 636
BbvI GCAGC 2 cut(s) 38, 487
BceAI ACGGC 1 cut(s) 34
BcgI CGANNNNNNTGC 2 cut(s) 181, 215
BciT130I CCWGG 2 cut(s) 183, 781
BcoDI GTCTC 4 cut(s) 61, 79, 391, 446
BfaI CTAG 1 cut(s) 98
BfmI CTRYAG 1 cut(s) 319
BfoI RGCGCY 1 cut(s) 501
BisI GCNGC 7 cut(s) 43, 52, 75, 214, 263, 374, 501
BlsI GCNGC 7 cut(s) 44, 53, 76, 215, 264, 375, 502
Bme1390I CCNGG 2 cut(s) 183, 781
Bme18I GGWCC 1 cut(s) 411
BmeT110I CYCGRG 1 cut(s) 960
BmgT120I GGNCC 5 cut(s) 40, 72, 411, 484, 635
BmiI GGNNCC 2 cut(s) 41, 205
BmrFI CCNGG 2 cut(s) 183, 781
BmsI GCATC 5 cut(s) 63, 596, 638, 831, 979
BpiI GAAGAC 1 cut(s) 636
Bpu10I CCTNAGC 1 cut(s) 993
BpuEI CTTGAG 3 cut(s) 65, 428, 935
BsaAI YACGTR 1 cut(s) 241
BsaI GGTCTC 2 cut(s) 79, 446
BsaJI CCNNGG 4 cut(s) 199, 414, 779, 780
BsaXI ACNNNNNCTCC 2 cut(s) 683, 713
Bsc4I CCNNNNNNNGG 2 cut(s) 851, 944
Bse1I ACTGG 7 cut(s) 46, 169, 319, 361, 535, 628, 882
Bse3DI GCAATG 3 cut(s) 192, 588, 1037
BseBI CCWGG 2 cut(s) 183, 781
BseDI CCNNGG 4 cut(s) 199, 414, 779, 780
BseGI GGATG 3 cut(s) 307, 424, 994
BseLI CCNNNNNNNGG 2 cut(s) 851, 944
BseMI GCAATG 3 cut(s) 192, 588, 1037
BseNI ACTGG 7 cut(s) 46, 169, 319, 361, 535, 628, 882
BseRI GAGGAG 2 cut(s) 152, 155
BseXI GCAGC 2 cut(s) 38, 487
BseYI CCCAGC 1 cut(s) 943
BsgI GTGCAG 1 cut(s) 486
BshFI GGCC 6 cut(s) 42, 74, 485, 637, 902, 954
BshNI GGYRCC 1 cut(s) 203
BsiHKCI CYCGRG 1 cut(s) 960
BsiSI CCGG 2 cut(s) 210, 955
BslFI GGGAC 2 cut(s) 338, 868
BslI CCNNNNNNNGG 2 cut(s) 851, 944
BsmAI GTCTC 4 cut(s) 61, 79, 391, 446
BsmBI CGTCTC 1 cut(s) 61
BsmFI GGGAC 2 cut(s) 338, 868
BsmI GAATGC 1 cut(s) 713
BsnI GGCC 6 cut(s) 42, 74, 485, 637, 902, 954
Bso31I GGTCTC 2 cut(s) 79, 446
BsoBI CYCGRG 1 cut(s) 960
Bsp143I GATC 2 cut(s) 280, 490
Bsp19I CCATGG 1 cut(s) 414
BspACI CCGC 6 cut(s) 35, 43, 75, 213, 262, 374
BspANI GGCC 6 cut(s) 42, 74, 485, 637, 902, 954
BspHI TCATGA 1 cut(s) 61
BspLI GGNNCC 2 cut(s) 41, 205
BspPI GGATC 1 cut(s) 288
BspT107I GGYRCC 1 cut(s) 203
BspTNI GGTCTC 2 cut(s) 79, 446
BsrDI GCAATG 3 cut(s) 192, 588, 1037
BsrI ACTGG 7 cut(s) 46, 169, 319, 361, 535, 628, 882
BssECI CCNNGG 4 cut(s) 199, 414, 779, 780
BssMI GATC 2 cut(s) 280, 490
BssSI CACGAG 1 cut(s) 744
BssT1I CCWWGG 1 cut(s) 414
Bst2BI CACGAG 1 cut(s) 744
Bst2UI CCWGG 2 cut(s) 183, 781
Bst4CI ACNGT 6 cut(s) 229, 275, 343, 507, 562, 867
Bst6I CTCTTC 1 cut(s) 290
BstBAI YACGTR 1 cut(s) 241
BstC8I GCNNGC 3 cut(s) 23, 525, 1028
BstDEI CTNAG 1 cut(s) 993
BstDSI CCRYGG 1 cut(s) 414
BstF5I GGATG 3 cut(s) 307, 424, 994
BstH2I RGCGCY 1 cut(s) 501
BstHHI GCGC 2 cut(s) 192, 500
BstKTI GATC 2 cut(s) 283, 493
BstMAI GTCTC 4 cut(s) 61, 79, 391, 446
BstMBI GATC 2 cut(s) 280, 490
BstMWI GCNNNNNNNGC 6 cut(s) 48, 51, 187, 203, 315, 919
BstNI CCWGG 2 cut(s) 183, 781
BstNSI RCATGY 1 cut(s) 861
BstSCI CCNGG 2 cut(s) 181, 779
BstSFI CTRYAG 1 cut(s) 319
BstSNI TACGTA 1 cut(s) 241
BstV1I GCAGC 2 cut(s) 38, 487
BstV2I GAAGAC 1 cut(s) 636
BstX2I RGATCY 1 cut(s) 280
BstYI RGATCY 1 cut(s) 280
BsuRI GGCC 6 cut(s) 42, 74, 485, 637, 902, 954
BtgI CCRYGG 1 cut(s) 414
BtsCI GGATG 3 cut(s) 307, 424, 994
BtsIMutI CAGTG 6 cut(s) 53, 225, 303, 503, 621, 702
Cac8I GCNNGC 3 cut(s) 23, 525, 1028
CciI TCATGA 1 cut(s) 61
CfoI GCGC 2 cut(s) 192, 500
Cfr13I GGNCC 5 cut(s) 40, 72, 411, 484, 635
Csp6I GTAC 1 cut(s) 391
CspCI CAANNNNNGTGG 2 cut(s) 1015, 1050
CviAII CATG 3 cut(s) 62, 415, 858
CviQI GTAC 1 cut(s) 391
DdeI CTNAG 1 cut(s) 993
DpnI GATC 2 cut(s) 282, 492
DpnII GATC 2 cut(s) 280, 490
DraI TTTAAA 1 cut(s) 661
EaeI YGGCCR 1 cut(s) 952
Eam1104I CTCTTC 1 cut(s) 290
EarI CTCTTC 1 cut(s) 290
Eco105I TACGTA 1 cut(s) 241
Eco130I CCWWGG 1 cut(s) 414
Eco147I AGGCCT 1 cut(s) 902
Eco31I GGTCTC 2 cut(s) 79, 446
Eco47I GGWCC 1 cut(s) 411
Eco88I CYCGRG 1 cut(s) 960
EcoO109I RGGNCCY 1 cut(s) 484
EcoRII CCWGG 2 cut(s) 181, 779
EcoT14I CCWWGG 1 cut(s) 414
EcoT22I ATGCAT 1 cut(s) 713
ErhI CCWWGG 1 cut(s) 414
Esp3I CGTCTC 1 cut(s) 61
FaeI CATG 3 cut(s) 65, 418, 861
FaiI YATR 7 cut(s) 63, 366, 405, 416, 537, 672, 859
FaqI GGGAC 2 cut(s) 338, 868
FatI CATG 3 cut(s) 61, 414, 857
FauI CCCGC 1 cut(s) 28
FblI GTMKAC 1 cut(s) 516
Fnu4HI GCNGC 7 cut(s) 43, 52, 75, 214, 263, 374, 501
FokI GGATG 3 cut(s) 314, 431, 1001
Fsp4HI GCNGC 7 cut(s) 43, 52, 75, 214, 263, 374, 501
FspBI CTAG 1 cut(s) 98
GlaI GCGC 2 cut(s) 191, 499
GluI GCNGC 7 cut(s) 43, 52, 75, 214, 263, 374, 501
GsaI CCCAGC 1 cut(s) 947
HaeII RGCGCY 1 cut(s) 501
HaeIII GGCC 6 cut(s) 42, 74, 485, 637, 902, 954
HapII CCGG 2 cut(s) 210, 955
HhaI GCGC 2 cut(s) 192, 500
Hin1II CATG 3 cut(s) 65, 418, 861
Hin6I GCGC 2 cut(s) 190, 498
HinP1I GCGC 2 cut(s) 190, 498
HindIII AAGCTT 1 cut(s) 521
HinfI GANTC 6 cut(s) 5, 145, 221, 329, 771, 958
HpaII CCGG 2 cut(s) 210, 955
HphI GGTGA 4 cut(s) 197, 596, 638, 711
Hpy166II GTNNAC 3 cut(s) 411, 517, 863
Hpy188I TCNGA 5 cut(s) 250, 776, 821, 888, 1052
Hpy188III TCNNGA 6 cut(s) 62, 82, 278, 335, 445, 746
Hpy8I GTNNAC 3 cut(s) 411, 517, 863
Hpy99I CGWCG 3 cut(s) 20, 164, 480
HpyAV CCTTC 5 cut(s) 25, 469, 505, 670, 979
HpyCH4III ACNGT 6 cut(s) 229, 275, 343, 507, 562, 867
HpyCH4IV ACGT 1 cut(s) 240
HpyF10VI GCNNNNNNNGC 6 cut(s) 48, 51, 187, 203, 315, 919
HpyF3I CTNAG 1 cut(s) 993
HpySE526I ACGT 1 cut(s) 240
Hsp92II CATG 3 cut(s) 65, 418, 861
HspAI GCGC 2 cut(s) 190, 498
Kzo9I GATC 2 cut(s) 280, 490
Lsp1109I GCAGC 2 cut(s) 38, 487
LweI GCATC 5 cut(s) 63, 596, 638, 831, 979
MaeI CTAG 1 cut(s) 98
MaeII ACGT 1 cut(s) 240
MaeIII GTNAC 3 cut(s) 584, 619, 872
MalI GATC 2 cut(s) 282, 492
MboI GATC 2 cut(s) 280, 490
MboII GAAGA 3 cut(s) 40, 277, 641
MfeI CAATTG 2 cut(s) 451, 615
MflI RGATCY 1 cut(s) 280
MluCI AATT 8 cut(s) 251, 268, 451, 615, 639, 814, 822, 948
MlyI GAGTC 2 cut(s) 14, 952
Mph1103I ATGCAT 1 cut(s) 713
MroXI GAANNNNTTC 1 cut(s) 561
MseI TTAA 4 cut(s) 177, 599, 660, 969
MspA1I CMGCKG 1 cut(s) 376
MspI CCGG 2 cut(s) 210, 955
MspR9I CCNGG 2 cut(s) 183, 781
MunI CAATTG 2 cut(s) 451, 615
Mva1269I GAATGC 1 cut(s) 713
MvaI CCWGG 2 cut(s) 183, 781
MwoI GCNNNNNNNGC 6 cut(s) 48, 51, 187, 203, 315, 919
NcoI CCATGG 1 cut(s) 414
NdeII GATC 2 cut(s) 280, 490
NlaIII CATG 3 cut(s) 65, 418, 861
NlaIV GGNNCC 2 cut(s) 41, 205
NmuCI GTSAC 2 cut(s) 584, 619
NsiI ATGCAT 1 cut(s) 713
NspI RCATGY 1 cut(s) 861
PaeR7I CTCGAG 1 cut(s) 960
PagI TCATGA 1 cut(s) 61
PasI CCCWGGG 1 cut(s) 780
PceI AGGCCT 1 cut(s) 902
PciI ACATGT 1 cut(s) 857
PcsI WCGNNNNNNNCGW 1 cut(s) 246
PctI GAATGC 1 cut(s) 713
PdmI GAANNNNTTC 1 cut(s) 561
PfeI GAWTC 4 cut(s) 145, 221, 329, 771
PkrI GCNGC 7 cut(s) 44, 53, 76, 215, 264, 375, 502
PleI GAGTC 2 cut(s) 13, 952
PpsI GAGTC 2 cut(s) 13, 952
Ppu21I YACGTR 1 cut(s) 241
PscI ACATGT 1 cut(s) 857
Psp6I CCWGG 2 cut(s) 181, 779
PspFI CCCAGC 1 cut(s) 943
PspGI CCWGG 2 cut(s) 181, 779
PspN4I GGNNCC 2 cut(s) 41, 205
PspPI GGNCC 5 cut(s) 40, 72, 411, 484, 635
PspXI VCTCGAGB 1 cut(s) 960
PsuI RGATCY 1 cut(s) 280
RsaI GTAC 1 cut(s) 392
RsaNI GTAC 1 cut(s) 391
SaqAI TTAA 4 cut(s) 177, 599, 660, 969
SatI GCNGC 7 cut(s) 43, 52, 75, 214, 263, 374, 501
Sau3AI GATC 2 cut(s) 280, 490
Sau96I GGNCC 5 cut(s) 40, 72, 411, 484, 635
SchI GAGTC 2 cut(s) 14, 952
ScrFI CCNGG 2 cut(s) 183, 781
SfaNI GCATC 5 cut(s) 63, 596, 638, 831, 979
SfcI CTRYAG 1 cut(s) 319
Sfr274I CTCGAG 1 cut(s) 960
SinI GGWCC 1 cut(s) 411
SlaI CTCGAG 1 cut(s) 960
SmlI CTYRAG 4 cut(s) 80, 443, 914, 960
SmoI CTYRAG 4 cut(s) 80, 443, 914, 960
SnaBI TACGTA 1 cut(s) 241
Sse9I AATT 8 cut(s) 251, 268, 451, 615, 639, 814, 822, 948
SseBI AGGCCT 1 cut(s) 902
SsiI CCGC 6 cut(s) 35, 43, 75, 213, 262, 374
SspMI CTAG 1 cut(s) 98
StuI AGGCCT 1 cut(s) 902
StyD4I CCNGG 2 cut(s) 181, 779
StyI CCWWGG 1 cut(s) 414
TaaI ACNGT 6 cut(s) 229, 275, 343, 507, 562, 867
TaiI ACGT 1 cut(s) 243
TaqI TCGA 3 cut(s) 162, 961, 1020
TasI AATT 8 cut(s) 251, 268, 451, 615, 639, 814, 822, 948
TauI GCSGC 5 cut(s) 45, 77, 216, 265, 376
TfiI GAWTC 4 cut(s) 145, 221, 329, 771
Tru1I TTAA 4 cut(s) 177, 599, 660, 969
Tru9I TTAA 4 cut(s) 177, 599, 660, 969
TscAI CASTG 6 cut(s) 53, 232, 303, 510, 628, 709
TseFI GTSAC 2 cut(s) 584, 619
TseI GCWGC 2 cut(s) 51, 500
Tsp45I GTSAC 2 cut(s) 584, 619
TspDTI ATGAA 4 cut(s) 137, 369, 435, 742
TspRI CASTG 6 cut(s) 53, 232, 303, 510, 628, 709
VpaK11BI GGWCC 1 cut(s) 411
XapI RAATTY 2 cut(s) 814, 822
XceI RCATGY 1 cut(s) 861
XhoI CTCGAG 1 cut(s) 960
XmiI GTMKAC 1 cut(s) 516
XmnI GAANNNNTTC 1 cut(s) 561
XspI CTAG 1 cut(s) 98
Zsp2I ATGCAT 1 cut(s) 713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.