Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G76170 AT1G76170 AT1G76170 AT1G76170 AT1G76170 AT1G76170 AT1G76170 AT2G44270 |
| fragaria_vesca | FvH4_2g37771 FvH4_2g37771 FvH4_2g37771 |
| malus_domestica | MD08G1004800.v1.1 MD15G1004300.v1.1 |
| prunus_persica | Prupe.1G356900_v2.0.a1 |
| pyrus_communis | pycom08g00360 pycom08g00370 pycom15g00310 pycom15g00320 |
| rosa_chinensis | RchiOBHm_Chr6g0303951 RchiOBHm_Chr6g0303961 |
| rosa_laevigata | RLG00000011030 |
| rosa_multiflora | Rmu_sc0004644.1_g000013 Rmu_sc0004644.1_g000014 |
| rosa_roxburghii | Rroxscaffold_7G00164230 |
| rosa_rugosa | Rorug06G0326200 |
| rosa_samantha | Rh6AG440500 Rh6BG462400 Rh6CG452800 Rh6DG439800 |
| rosa_wichuraiana | Rw6G038150 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 203 |
| AciI | CCGC | 6 cut(s) | 35, 43, 75, 213, 262, 477 |
| AclWI | GGATC | 1 cut(s) | 288 |
| AcsI | RAATTY | 1 cut(s) | 386 |
| AgsI | TTSAA | 2 cut(s) | 256, 340 |
| AjnI | CCWGG | 2 cut(s) | 181, 362 |
| AluBI | AGCT | 1 cut(s) | 431 |
| AluI | AGCT | 1 cut(s) | 431 |
| Alw26I | GTCTC | 3 cut(s) | 61, 79, 494 |
| AlwI | GGATC | 1 cut(s) | 288 |
| AoxI | GGCC | 2 cut(s) | 40, 72 |
| ApeKI | GCWGC | 1 cut(s) | 51 |
| ApoI | RAATTY | 1 cut(s) | 386 |
| AspLEI | GCGC | 1 cut(s) | 192 |
| AspS9I | GGNCC | 3 cut(s) | 40, 72, 514 |
| AsuHPI | GGTGA | 1 cut(s) | 197 |
| AvaII | GGWCC | 1 cut(s) | 514 |
| BanI | GGYRCC | 1 cut(s) | 203 |
| BbvI | GCAGC | 1 cut(s) | 38 |
| BccI | CCATC | 1 cut(s) | 298 |
| BceAI | ACGGC | 1 cut(s) | 34 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 181, 215 |
| BciT130I | CCWGG | 2 cut(s) | 183, 364 |
| BcoDI | GTCTC | 3 cut(s) | 61, 79, 494 |
| BfaI | CTAG | 1 cut(s) | 98 |
| BfmI | CTRYAG | 1 cut(s) | 321 |
| BisI | GCNGC | 5 cut(s) | 43, 52, 75, 263, 477 |
| BlsI | GCNGC | 5 cut(s) | 44, 53, 76, 264, 478 |
| Bme1390I | CCNGG | 2 cut(s) | 183, 364 |
| Bme18I | GGWCC | 1 cut(s) | 514 |
| BmgT120I | GGNCC | 3 cut(s) | 40, 72, 514 |
| BmiI | GGNNCC | 2 cut(s) | 41, 205 |
| BmrFI | CCNGG | 2 cut(s) | 183, 364 |
| BmsI | GCATC | 1 cut(s) | 63 |
| BpuEI | CTTGAG | 1 cut(s) | 65 |
| Bsa29I | ATCGAT | 1 cut(s) | 283 |
| BsaBI | GATNNNNATC | 2 cut(s) | 285, 289 |
| BsaI | GGTCTC | 1 cut(s) | 79 |
| BsaJI | CCNNGG | 2 cut(s) | 199, 517 |
| Bse1I | ACTGG | 3 cut(s) | 46, 169, 321 |
| Bse3DI | GCAATG | 1 cut(s) | 192 |
| Bse8I | GATNNNNATC | 2 cut(s) | 285, 289 |
| BseBI | CCWGG | 2 cut(s) | 183, 364 |
| BseCI | ATCGAT | 1 cut(s) | 283 |
| BseDI | CCNNGG | 2 cut(s) | 199, 517 |
| BseJI | GATNNNNATC | 2 cut(s) | 285, 289 |
| BseMI | GCAATG | 1 cut(s) | 192 |
| BseNI | ACTGG | 3 cut(s) | 46, 169, 321 |
| BseXI | GCAGC | 1 cut(s) | 38 |
| BshFI | GGCC | 2 cut(s) | 42, 74 |
| BshNI | GGYRCC | 1 cut(s) | 203 |
| BshVI | ATCGAT | 1 cut(s) | 283 |
| BsiSI | CCGG | 1 cut(s) | 210 |
| BslFI | GGGAC | 1 cut(s) | 340 |
| BsmAI | GTCTC | 3 cut(s) | 61, 79, 494 |
| BsmBI | CGTCTC | 1 cut(s) | 61 |
| BsmFI | GGGAC | 1 cut(s) | 340 |
| BsnI | GGCC | 2 cut(s) | 42, 74 |
| Bso31I | GGTCTC | 1 cut(s) | 79 |
| Bsp143I | GATC | 1 cut(s) | 280 |
| Bsp19I | CCATGG | 1 cut(s) | 517 |
| BspACI | CCGC | 6 cut(s) | 35, 43, 75, 213, 262, 477 |
| BspANI | GGCC | 2 cut(s) | 42, 74 |
| BspDI | ATCGAT | 1 cut(s) | 283 |
| BspHI | TCATGA | 1 cut(s) | 61 |
| BspLI | GGNNCC | 2 cut(s) | 41, 205 |
| BspPI | GGATC | 1 cut(s) | 288 |
| BspT107I | GGYRCC | 1 cut(s) | 203 |
| BspTNI | GGTCTC | 1 cut(s) | 79 |
| BsrDI | GCAATG | 1 cut(s) | 192 |
| BsrI | ACTGG | 3 cut(s) | 46, 169, 321 |
| BssECI | CCNNGG | 2 cut(s) | 199, 517 |
| BssMI | GATC | 1 cut(s) | 280 |
| BssT1I | CCWWGG | 1 cut(s) | 517 |
| Bst2UI | CCWGG | 2 cut(s) | 183, 364 |
| Bst4CI | ACNGT | 3 cut(s) | 229, 275, 345 |
| BstC8I | GCNNGC | 1 cut(s) | 23 |
| BstDSI | CCRYGG | 1 cut(s) | 517 |
| BstHHI | GCGC | 1 cut(s) | 192 |
| BstKTI | GATC | 1 cut(s) | 283 |
| BstMAI | GTCTC | 3 cut(s) | 61, 79, 494 |
| BstMBI | GATC | 1 cut(s) | 280 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 48, 51, 187, 203, 317 |
| BstNI | CCWGG | 2 cut(s) | 183, 364 |
| BstSCI | CCNGG | 2 cut(s) | 181, 362 |
| BstSFI | CTRYAG | 1 cut(s) | 321 |
| BstV1I | GCAGC | 1 cut(s) | 38 |
| Bsu15I | ATCGAT | 1 cut(s) | 283 |
| BsuRI | GGCC | 2 cut(s) | 42, 74 |
| BsuTUI | ATCGAT | 1 cut(s) | 283 |
| BtgI | CCRYGG | 1 cut(s) | 517 |
| BtsIMutI | CAGTG | 2 cut(s) | 53, 225 |
| Cac8I | GCNNGC | 1 cut(s) | 23 |
| CciI | TCATGA | 1 cut(s) | 61 |
| CfoI | GCGC | 1 cut(s) | 192 |
| Cfr13I | GGNCC | 3 cut(s) | 40, 72, 514 |
| ClaI | ATCGAT | 1 cut(s) | 283 |
| CviAII | CATG | 3 cut(s) | 62, 397, 518 |
| CviJI | RGCY | 7 cut(s) | 14, 25, 42, 74, 181, 320, 431 |
| CviKI_1 | RGCY | 7 cut(s) | 14, 25, 42, 74, 181, 320, 431 |
| DpnI | GATC | 1 cut(s) | 282 |
| DpnII | GATC | 1 cut(s) | 280 |
| DraI | TTTAAA | 1 cut(s) | 427 |
| Eco130I | CCWWGG | 1 cut(s) | 517 |
| Eco31I | GGTCTC | 1 cut(s) | 79 |
| Eco32I | GATATC | 1 cut(s) | 287 |
| Eco47I | GGWCC | 1 cut(s) | 514 |
| EcoRII | CCWGG | 2 cut(s) | 181, 362 |
| EcoRV | GATATC | 1 cut(s) | 287 |
| EcoT14I | CCWWGG | 1 cut(s) | 517 |
| ErhI | CCWWGG | 1 cut(s) | 517 |
| Esp3I | CGTCTC | 1 cut(s) | 61 |
| FaeI | CATG | 3 cut(s) | 65, 400, 521 |
| FaiI | YATR | 7 cut(s) | 63, 240, 384, 398, 469, 508, 519 |
| FaqI | GGGAC | 1 cut(s) | 340 |
| FatI | CATG | 3 cut(s) | 61, 396, 517 |
| FauI | CCCGC | 1 cut(s) | 28 |
| Fnu4HI | GCNGC | 5 cut(s) | 43, 52, 75, 263, 477 |
| Fsp4HI | GCNGC | 5 cut(s) | 43, 52, 75, 263, 477 |
| FspBI | CTAG | 1 cut(s) | 98 |
| GlaI | GCGC | 1 cut(s) | 191 |
| GluI | GCNGC | 5 cut(s) | 43, 52, 75, 263, 477 |
| HaeIII | GGCC | 2 cut(s) | 42, 74 |
| HapII | CCGG | 1 cut(s) | 210 |
| HhaI | GCGC | 1 cut(s) | 192 |
| Hin1II | CATG | 3 cut(s) | 65, 400, 521 |
| Hin6I | GCGC | 1 cut(s) | 190 |
| HinP1I | GCGC | 1 cut(s) | 190 |
| HindIII | AAGCTT | 1 cut(s) | 429 |
| HinfI | GANTC | 6 cut(s) | 5, 145, 221, 331, 440, 452 |
| HpaII | CCGG | 1 cut(s) | 210 |
| HphI | GGTGA | 1 cut(s) | 197 |
| Hpy166II | GTNNAC | 1 cut(s) | 514 |
| Hpy188I | TCNGA | 1 cut(s) | 250 |
| Hpy188III | TCNNGA | 3 cut(s) | 62, 82, 337 |
| Hpy8I | GTNNAC | 1 cut(s) | 514 |
| Hpy99I | CGWCG | 2 cut(s) | 20, 164 |
| HpyAV | CCTTC | 1 cut(s) | 25 |
| HpyCH4III | ACNGT | 3 cut(s) | 229, 275, 345 |
| HpyCH4V | TGCA | 2 cut(s) | 54, 111 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 48, 51, 187, 203, 317 |
| Hsp92II | CATG | 3 cut(s) | 65, 400, 521 |
| HspAI | GCGC | 1 cut(s) | 190 |
| Kzo9I | GATC | 1 cut(s) | 280 |
| Lsp1109I | GCAGC | 1 cut(s) | 38 |
| LweI | GCATC | 1 cut(s) | 63 |
| MaeI | CTAG | 1 cut(s) | 98 |
| MalI | GATC | 1 cut(s) | 282 |
| MboI | GATC | 1 cut(s) | 280 |
| MboII | GAAGA | 1 cut(s) | 40 |
| MluCI | AATT | 5 cut(s) | 251, 268, 373, 386, 435 |
| MlyI | GAGTC | 2 cut(s) | 14, 434 |
| MnlI | CCTC | 6 cut(s) | 89, 121, 209, 217, 242, 300 |
| MseI | TTAA | 3 cut(s) | 177, 426, 434 |
| MspA1I | CMGCKG | 1 cut(s) | 479 |
| MspI | CCGG | 1 cut(s) | 210 |
| MspR9I | CCNGG | 2 cut(s) | 183, 364 |
| MvaI | CCWGG | 2 cut(s) | 183, 364 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 48, 51, 187, 203, 317 |
| NcoI | CCATGG | 1 cut(s) | 517 |
| NdeII | GATC | 1 cut(s) | 280 |
| NlaIII | CATG | 3 cut(s) | 65, 400, 521 |
| NlaIV | GGNNCC | 2 cut(s) | 41, 205 |
| PagI | TCATGA | 1 cut(s) | 61 |
| PfeI | GAWTC | 4 cut(s) | 145, 221, 331, 452 |
| PkrI | GCNGC | 5 cut(s) | 44, 53, 76, 264, 478 |
| PleI | GAGTC | 2 cut(s) | 13, 434 |
| PpsI | GAGTC | 2 cut(s) | 13, 434 |
| Psp6I | CCWGG | 2 cut(s) | 181, 362 |
| PspGI | CCWGG | 2 cut(s) | 181, 362 |
| PspN4I | GGNNCC | 2 cut(s) | 41, 205 |
| PspPI | GGNCC | 3 cut(s) | 40, 72, 514 |
| SaqAI | TTAA | 3 cut(s) | 177, 426, 434 |
| SatI | GCNGC | 5 cut(s) | 43, 52, 75, 263, 477 |
| Sau3AI | GATC | 1 cut(s) | 280 |
| Sau96I | GGNCC | 3 cut(s) | 40, 72, 514 |
| SchI | GAGTC | 2 cut(s) | 14, 434 |
| ScrFI | CCNGG | 2 cut(s) | 183, 364 |
| SetI | ASST | 4 cut(s) | 132, 156, 368, 433 |
| SfaNI | GCATC | 1 cut(s) | 63 |
| SfcI | CTRYAG | 1 cut(s) | 321 |
| SinI | GGWCC | 1 cut(s) | 514 |
| SmlI | CTYRAG | 1 cut(s) | 80 |
| SmoI | CTYRAG | 1 cut(s) | 80 |
| Sse9I | AATT | 5 cut(s) | 251, 268, 373, 386, 435 |
| SsiI | CCGC | 6 cut(s) | 35, 43, 75, 213, 262, 477 |
| SspMI | CTAG | 1 cut(s) | 98 |
| StyD4I | CCNGG | 2 cut(s) | 181, 362 |
| StyI | CCWWGG | 1 cut(s) | 517 |
| TaaI | ACNGT | 3 cut(s) | 229, 275, 345 |
| TaqI | TCGA | 3 cut(s) | 162, 283, 378 |
| TaqII | GACCGA | 1 cut(s) | 382 |
| TasI | AATT | 5 cut(s) | 251, 268, 373, 386, 435 |
| TauI | GCSGC | 4 cut(s) | 45, 77, 265, 479 |
| TfiI | GAWTC | 4 cut(s) | 145, 221, 331, 452 |
| Tru1I | TTAA | 3 cut(s) | 177, 426, 434 |
| Tru9I | TTAA | 3 cut(s) | 177, 426, 434 |
| TscAI | CASTG | 2 cut(s) | 53, 232 |
| TseI | GCWGC | 1 cut(s) | 51 |
| TspDTI | ATGAA | 3 cut(s) | 137, 371, 399 |
| TspRI | CASTG | 2 cut(s) | 53, 232 |
| VpaK11BI | GGWCC | 1 cut(s) | 514 |
| XapI | RAATTY | 1 cut(s) | 386 |
| XspI | CTAG | 1 cut(s) | 98 |