Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G76170 AT1G76170 AT1G76170 AT1G76170 AT1G76170 AT1G76170 AT1G76170 AT2G44270 |
| fragaria_vesca | FvH4_2g37771 FvH4_2g37771 FvH4_2g37771 |
| malus_domestica | MD08G1004800.v1.1 MD15G1004300.v1.1 |
| prunus_persica | Prupe.1G356900_v2.0.a1 |
| pyrus_communis | pycom08g00360 pycom08g00370 pycom15g00310 pycom15g00320 |
| rosa_chinensis | RchiOBHm_Chr6g0303951 RchiOBHm_Chr6g0303961 |
| rosa_laevigata | RLG00000011030 |
| rosa_multiflora | Rmu_sc0004644.1_g000013 Rmu_sc0004644.1_g000014 |
| rosa_roxburghii | Rroxscaffold_7G00164230 |
| rosa_rugosa | Rorug06G0326200 |
| rosa_samantha | Rh6AG440500 Rh6BG462400 Rh6CG452800 Rh6DG439800 |
| rosa_wichuraiana | Rw6G038150 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 203 |
| AccI | GTMKAC | 1 cut(s) | 516 |
| AccII | CGCG | 1 cut(s) | 495 |
| AciI | CCGC | 2 cut(s) | 262, 495 |
| AclWI | GGATC | 1 cut(s) | 288 |
| AcsI | RAATTY | 2 cut(s) | 820, 828 |
| AfaI | GTAC | 1 cut(s) | 392 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 857, 950 |
| AflIII | ACRYGT | 1 cut(s) | 863 |
| AgsI | TTSAA | 7 cut(s) | 164, 256, 338, 511, 571, 615, 888 |
| AluBI | AGCT | 5 cut(s) | 74, 523, 554, 695, 908 |
| AluI | AGCT | 5 cut(s) | 74, 523, 554, 695, 908 |
| Alw26I | GTCTC | 1 cut(s) | 446 |
| AlwI | GGATC | 1 cut(s) | 288 |
| AoxI | GGCC | 3 cut(s) | 86, 483, 789 |
| ApeKI | GCWGC | 5 cut(s) | 14, 51, 74, 500, 695 |
| ApoI | RAATTY | 2 cut(s) | 820, 828 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 220, 561 |
| AspA2I | CCTAGG | 1 cut(s) | 785 |
| AspLEI | GCGC | 1 cut(s) | 41 |
| AspS9I | GGNCC | 4 cut(s) | 87, 484, 635, 789 |
| AsuC2I | CCSGG | 1 cut(s) | 183 |
| AsuHPI | GGTGA | 5 cut(s) | 166, 197, 596, 638, 717 |
| AvaII | GGWCC | 1 cut(s) | 635 |
| AvrII | CCTAGG | 1 cut(s) | 785 |
| BanI | GGYRCC | 1 cut(s) | 203 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 595, 627 |
| BauI | CACGAG | 1 cut(s) | 750 |
| BbvI | GCAGC | 4 cut(s) | 38, 61, 487, 682 |
| BccI | CCATC | 3 cut(s) | 409, 626, 943 |
| BceAI | ACGGC | 1 cut(s) | 358 |
| BcnI | CCSGG | 1 cut(s) | 183 |
| BcoDI | GTCTC | 1 cut(s) | 446 |
| BfaI | CTAG | 2 cut(s) | 98, 786 |
| BfmI | CTRYAG | 1 cut(s) | 319 |
| BfoI | RGCGCY | 1 cut(s) | 42 |
| BisI | GCNGC | 6 cut(s) | 15, 52, 75, 263, 501, 696 |
| BlnI | CCTAGG | 1 cut(s) | 785 |
| BlsI | GCNGC | 6 cut(s) | 16, 53, 76, 264, 502, 697 |
| Bme1390I | CCNGG | 1 cut(s) | 183 |
| Bme18I | GGWCC | 1 cut(s) | 635 |
| BmgT120I | GGNCC | 4 cut(s) | 87, 484, 635, 789 |
| BmiI | GGNNCC | 1 cut(s) | 205 |
| BmrFI | CCNGG | 1 cut(s) | 183 |
| BmsI | GCATC | 3 cut(s) | 63, 596, 638 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 63, 95 |
| BpuEI | CTTGAG | 3 cut(s) | 65, 428, 941 |
| BpuMI | CCSGG | 1 cut(s) | 183 |
| BsaAI | YACGTR | 1 cut(s) | 581 |
| BsaI | GGTCTC | 1 cut(s) | 446 |
| BsaJI | CCNNGG | 1 cut(s) | 785 |
| BsaWI | WCCGGW | 2 cut(s) | 209, 530 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 857, 950 |
| Bse118I | RCCGGY | 1 cut(s) | 17 |
| Bse1I | ACTGG | 3 cut(s) | 46, 169, 628 |
| Bse3DI | GCAATG | 1 cut(s) | 192 |
| BseDI | CCNNGG | 1 cut(s) | 785 |
| BseGI | GGATG | 3 cut(s) | 307, 331, 424 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 857, 950 |
| BseMI | GCAATG | 1 cut(s) | 192 |
| BseMII | CTCAG | 1 cut(s) | 998 |
| BseNI | ACTGG | 3 cut(s) | 46, 169, 628 |
| BseRI | GAGGAG | 2 cut(s) | 152, 155 |
| BseXI | GCAGC | 4 cut(s) | 38, 61, 487, 682 |
| Bsh1236I | CGCG | 1 cut(s) | 495 |
| BshFI | GGCC | 3 cut(s) | 88, 485, 791 |
| BshNI | GGYRCC | 1 cut(s) | 203 |
| BsiSI | CCGG | 6 cut(s) | 18, 36, 183, 210, 531, 851 |
| BslFI | GGGAC | 2 cut(s) | 874, 939 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 857, 950 |
| BsmAI | GTCTC | 1 cut(s) | 446 |
| BsmFI | GGGAC | 2 cut(s) | 874, 939 |
| BsnI | GGCC | 3 cut(s) | 88, 485, 791 |
| Bso31I | GGTCTC | 1 cut(s) | 446 |
| Bsp143I | GATC | 2 cut(s) | 280, 490 |
| BspACI | CCGC | 2 cut(s) | 262, 495 |
| BspANI | GGCC | 3 cut(s) | 88, 485, 791 |
| BspCNI | CTCAG | 1 cut(s) | 997 |
| BspFNI | CGCG | 1 cut(s) | 495 |
| BspLI | GGNNCC | 1 cut(s) | 205 |
| BspPI | GGATC | 1 cut(s) | 288 |
| BspT107I | GGYRCC | 1 cut(s) | 203 |
| BspTNI | GGTCTC | 1 cut(s) | 446 |
| BsrDI | GCAATG | 1 cut(s) | 192 |
| BsrFI | RCCGGY | 1 cut(s) | 17 |
| BsrI | ACTGG | 3 cut(s) | 46, 169, 628 |
| BssAI | RCCGGY | 1 cut(s) | 17 |
| BssECI | CCNNGG | 1 cut(s) | 785 |
| BssMI | GATC | 2 cut(s) | 280, 490 |
| BssSI | CACGAG | 1 cut(s) | 750 |
| BssT1I | CCWWGG | 1 cut(s) | 785 |
| Bst2BI | CACGAG | 1 cut(s) | 750 |
| Bst4CI | ACNGT | 3 cut(s) | 229, 343, 562 |
| Bst6I | CTCTTC | 1 cut(s) | 290 |
| BstBAI | YACGTR | 1 cut(s) | 581 |
| BstC8I | GCNNGC | 5 cut(s) | 19, 23, 43, 525, 715 |
| BstDEI | CTNAG | 2 cut(s) | 688, 984 |
| BstF5I | GGATG | 3 cut(s) | 307, 331, 424 |
| BstFNI | CGCG | 1 cut(s) | 495 |
| BstH2I | RGCGCY | 1 cut(s) | 42 |
| BstHHI | GCGC | 1 cut(s) | 41 |
| BstKTI | GATC | 2 cut(s) | 283, 493 |
| BstMAI | GTCTC | 1 cut(s) | 446 |
| BstMBI | GATC | 2 cut(s) | 280, 490 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 51, 203, 315 |
| BstNSI | RCATGY | 1 cut(s) | 867 |
| BstSCI | CCNGG | 1 cut(s) | 181 |
| BstSFI | CTRYAG | 1 cut(s) | 319 |
| BstUI | CGCG | 1 cut(s) | 495 |
| BstV1I | GCAGC | 4 cut(s) | 38, 61, 487, 682 |
| BstX2I | RGATCY | 1 cut(s) | 280 |
| BstYI | RGATCY | 1 cut(s) | 280 |
| BsuRI | GGCC | 3 cut(s) | 88, 485, 791 |
| BtsCI | GGATG | 3 cut(s) | 307, 331, 424 |
| BtsIMutI | CAGTG | 3 cut(s) | 53, 225, 621 |
| Cac8I | GCNNGC | 5 cut(s) | 19, 23, 43, 525, 715 |
| CfoI | GCGC | 1 cut(s) | 41 |
| Cfr10I | RCCGGY | 1 cut(s) | 17 |
| Cfr13I | GGNCC | 4 cut(s) | 87, 484, 635, 789 |
| Csp6I | GTAC | 1 cut(s) | 391 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 214, 249, 1021, 1056 |
| CviAII | CATG | 1 cut(s) | 864 |
| CviQI | GTAC | 1 cut(s) | 391 |
| DdeI | CTNAG | 2 cut(s) | 688, 984 |
| DpnI | GATC | 2 cut(s) | 282, 492 |
| DpnII | GATC | 2 cut(s) | 280, 490 |
| DraI | TTTAAA | 1 cut(s) | 661 |
| Eam1104I | CTCTTC | 1 cut(s) | 290 |
| EarI | CTCTTC | 1 cut(s) | 290 |
| Eco130I | CCWWGG | 1 cut(s) | 785 |
| Eco31I | GGTCTC | 1 cut(s) | 446 |
| Eco47I | GGWCC | 1 cut(s) | 635 |
| EcoO109I | RGGNCCY | 1 cut(s) | 484 |
| EcoT14I | CCWWGG | 1 cut(s) | 785 |
| ErhI | CCWWGG | 1 cut(s) | 785 |
| FaeI | CATG | 1 cut(s) | 867 |
| FaqI | GGGAC | 2 cut(s) | 874, 939 |
| FatI | CATG | 1 cut(s) | 863 |
| FblI | GTMKAC | 1 cut(s) | 516 |
| Fnu4HI | GCNGC | 6 cut(s) | 15, 52, 75, 263, 501, 696 |
| FokI | GGATG | 3 cut(s) | 314, 338, 431 |
| Fsp4HI | GCNGC | 6 cut(s) | 15, 52, 75, 263, 501, 696 |
| FspBI | CTAG | 2 cut(s) | 98, 786 |
| GlaI | GCGC | 1 cut(s) | 40 |
| GluI | GCNGC | 6 cut(s) | 15, 52, 75, 263, 501, 696 |
| HaeII | RGCGCY | 1 cut(s) | 42 |
| HaeIII | GGCC | 3 cut(s) | 88, 485, 791 |
| HapII | CCGG | 6 cut(s) | 18, 36, 183, 210, 531, 851 |
| HhaI | GCGC | 1 cut(s) | 41 |
| Hin1II | CATG | 1 cut(s) | 867 |
| Hin6I | GCGC | 1 cut(s) | 39 |
| HinP1I | GCGC | 1 cut(s) | 39 |
| HindIII | AAGCTT | 2 cut(s) | 521, 906 |
| HinfI | GANTC | 4 cut(s) | 221, 329, 777, 964 |
| HpaII | CCGG | 6 cut(s) | 18, 36, 183, 210, 531, 851 |
| HphI | GGTGA | 5 cut(s) | 166, 197, 596, 638, 717 |
| Hpy166II | GTNNAC | 4 cut(s) | 188, 411, 517, 869 |
| Hpy188I | TCNGA | 6 cut(s) | 250, 782, 827, 963, 987, 1058 |
| Hpy188III | TCNNGA | 4 cut(s) | 82, 335, 445, 752 |
| Hpy8I | GTNNAC | 4 cut(s) | 188, 411, 517, 869 |
| Hpy99I | CGWCG | 1 cut(s) | 480 |
| HpyAV | CCTTC | 2 cut(s) | 670, 985 |
| HpyCH4III | ACNGT | 3 cut(s) | 229, 343, 562 |
| HpyCH4IV | ACGT | 1 cut(s) | 580 |
| HpyCH4V | TGCA | 8 cut(s) | 54, 111, 361, 456, 593, 609, 651, 713 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 51, 203, 315 |
| HpyF3I | CTNAG | 2 cut(s) | 688, 984 |
| HpySE526I | ACGT | 1 cut(s) | 580 |
| Hsp92II | CATG | 1 cut(s) | 867 |
| HspAI | GCGC | 1 cut(s) | 39 |
| KroI | GCCGGC | 1 cut(s) | 17 |
| KroNI | GCCGGC | 1 cut(s) | 19 |
| Kzo9I | GATC | 2 cut(s) | 280, 490 |
| Lsp1109I | GCAGC | 4 cut(s) | 38, 61, 487, 682 |
| LweI | GCATC | 3 cut(s) | 63, 596, 638 |
| MaeI | CTAG | 2 cut(s) | 98, 786 |
| MaeII | ACGT | 1 cut(s) | 580 |
| MaeIII | GTNAC | 3 cut(s) | 59, 619, 878 |
| MalI | GATC | 2 cut(s) | 282, 492 |
| MboI | GATC | 2 cut(s) | 280, 490 |
| MboII | GAAGA | 3 cut(s) | 277, 641, 1000 |
| MfeI | CAATTG | 1 cut(s) | 615 |
| MflI | RGATCY | 1 cut(s) | 280 |
| MmeI | TCCRAC | 2 cut(s) | 258, 941 |
| MnlI | CCTC | 8 cut(s) | 78, 121, 130, 133, 217, 298, 916, 962 |
| MroNI | GCCGGC | 1 cut(s) | 17 |
| MroXI | GAANNNNTTC | 2 cut(s) | 220, 561 |
| MseI | TTAA | 5 cut(s) | 177, 377, 660, 705, 975 |
| MslI | CAYNNNNRTG | 1 cut(s) | 270 |
| MspI | CCGG | 6 cut(s) | 18, 36, 183, 210, 531, 851 |
| MspR9I | CCNGG | 1 cut(s) | 183 |
| MunI | CAATTG | 1 cut(s) | 615 |
| MvnI | CGCG | 1 cut(s) | 495 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 51, 203, 315 |
| NaeI | GCCGGC | 1 cut(s) | 19 |
| NciI | CCSGG | 1 cut(s) | 183 |
| NdeII | GATC | 2 cut(s) | 280, 490 |
| NgoMIV | GCCGGC | 1 cut(s) | 17 |
| NlaIII | CATG | 1 cut(s) | 867 |
| NlaIV | GGNNCC | 1 cut(s) | 205 |
| NmuCI | GTSAC | 1 cut(s) | 619 |
| NspI | RCATGY | 1 cut(s) | 867 |
| PciI | ACATGT | 1 cut(s) | 863 |
| PdiI | GCCGGC | 1 cut(s) | 19 |
| PdmI | GAANNNNTTC | 2 cut(s) | 220, 561 |
| PfeI | GAWTC | 4 cut(s) | 221, 329, 777, 964 |
| PkrI | GCNGC | 6 cut(s) | 16, 53, 76, 264, 502, 697 |
| Ppu21I | YACGTR | 1 cut(s) | 581 |
| PscI | ACATGT | 1 cut(s) | 863 |
| PspN4I | GGNNCC | 1 cut(s) | 205 |
| PspPI | GGNCC | 4 cut(s) | 87, 484, 635, 789 |
| PsuI | RGATCY | 1 cut(s) | 280 |
| RsaI | GTAC | 1 cut(s) | 392 |
| RsaNI | GTAC | 1 cut(s) | 391 |
| RseI | CAYNNNNRTG | 1 cut(s) | 270 |
| SaqAI | TTAA | 5 cut(s) | 177, 377, 660, 705, 975 |
| SatI | GCNGC | 6 cut(s) | 15, 52, 75, 263, 501, 696 |
| Sau3AI | GATC | 2 cut(s) | 280, 490 |
| Sau96I | GGNCC | 4 cut(s) | 87, 484, 635, 789 |
| ScrFI | CCNGG | 1 cut(s) | 183 |
| SfaNI | GCATC | 3 cut(s) | 63, 596, 638 |
| SfcI | CTRYAG | 1 cut(s) | 319 |
| SinI | GGWCC | 1 cut(s) | 635 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 270 |
| SmlI | CTYRAG | 3 cut(s) | 80, 443, 920 |
| SmoI | CTYRAG | 3 cut(s) | 80, 443, 920 |
| SsiI | CCGC | 2 cut(s) | 262, 495 |
| SspMI | CTAG | 2 cut(s) | 98, 786 |
| StyD4I | CCNGG | 1 cut(s) | 181 |
| StyI | CCWWGG | 1 cut(s) | 785 |
| TaaI | ACNGT | 3 cut(s) | 229, 343, 562 |
| TaiI | ACGT | 1 cut(s) | 583 |
| TaqI | TCGA | 1 cut(s) | 967 |
| TauI | GCSGC | 1 cut(s) | 265 |
| TfiI | GAWTC | 4 cut(s) | 221, 329, 777, 964 |
| Tru1I | TTAA | 5 cut(s) | 177, 377, 660, 705, 975 |
| Tru9I | TTAA | 5 cut(s) | 177, 377, 660, 705, 975 |
| TscAI | CASTG | 3 cut(s) | 53, 232, 628 |
| TseFI | GTSAC | 1 cut(s) | 619 |
| TseI | GCWGC | 5 cut(s) | 14, 51, 74, 500, 695 |
| Tsp45I | GTSAC | 1 cut(s) | 619 |
| TspDTI | ATGAA | 4 cut(s) | 369, 435, 748, 1013 |
| TspRI | CASTG | 3 cut(s) | 53, 232, 628 |
| VpaK11BI | GGWCC | 1 cut(s) | 635 |
| XapI | RAATTY | 2 cut(s) | 820, 828 |
| XceI | RCATGY | 1 cut(s) | 867 |
| XmaJI | CCTAGG | 1 cut(s) | 785 |
| XmiI | GTMKAC | 1 cut(s) | 516 |
| XmnI | GAANNNNTTC | 2 cut(s) | 220, 561 |
| XspI | CTAG | 2 cut(s) | 98, 786 |