RchiOBHm_Chr6g0303951

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0005079
Physical Location & Seq
Reverse (-)
63462518 .. 63464073
1556 bp
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UTR
Exon/CDS
Intron
PRQ27306

Sequence Viewer

Length: 390 bp
ATGCATACTTCAAGCGGCTGGATTACTTCTCCACTGAATGCATTTATTCACCAAATGCTTATCGTGGTTTTGCTCGTGAGTTCATCAAAGATTTGGAAAGAATCAGGTCTCCAACCTAGGGCCATACTTGACATAATCAAATCAGGGGAGAATTTCAGAATTTCTACTACTACAAAAATGCCGGAACAGGGGACATGTGAACGCTGTGGTTACATTTCAAGCCAGAAATGGTGTAAAGCTTGTGTATTGCTTGAGGGACTGAATAGGGGTTTGCCAAAGATGGGAATTGGTCGGAATCGAGGAGTTAACAACAATCAGAAGAAGGATATGAAACAAAGTAATGGAGCAAAAAATATAGAGAGCAAACAATGTGGGACTTTGGACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04122 map04122
Pfam Domains
Protein Families
ATP_bind_3 zn-ribbon_14

Protein Analysis

129

Amino Acids

14.33

Weight (kDa)

9.63

Isoelectric Point (pI)

53.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zn-ribbon_14 PF16503 64 - 94 3.6e-19 Zinc-ribbon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 15
AcsI RAATTY 2 cut(s) 151, 159
AfiI CCNNNNNNNGG 3 cut(s) 118, 188, 281
AflIII ACRYGT 1 cut(s) 194
AgsI TTSAA 2 cut(s) 12, 219
AluBI AGCT 1 cut(s) 239
AluI AGCT 1 cut(s) 239
Alw26I GTCTC 1 cut(s) 113
AoxI GGCC 1 cut(s) 120
ApoI RAATTY 2 cut(s) 151, 159
AspA2I CCTAGG 1 cut(s) 116
AspS9I GGNCC 1 cut(s) 120
AsuHPI GGTGA 1 cut(s) 41
AvrII CCTAGG 1 cut(s) 116
BauI CACGAG 1 cut(s) 74
BccI CCATC 1 cut(s) 274
BcoDI GTCTC 1 cut(s) 113
BfaI CTAG 1 cut(s) 117
BisI GCNGC 1 cut(s) 16
BlnI CCTAGG 1 cut(s) 116
BlsI GCNGC 1 cut(s) 17
BmgT120I GGNCC 1 cut(s) 120
BpuEI CTTGAG 1 cut(s) 272
BsaI GGTCTC 1 cut(s) 113
BsaJI CCNNGG 1 cut(s) 116
Bsc4I CCNNNNNNNGG 3 cut(s) 118, 188, 281
BseDI CCNNGG 1 cut(s) 116
BseLI CCNNNNNNNGG 3 cut(s) 118, 188, 281
BseRI GAGGAG 1 cut(s) 315
BshFI GGCC 1 cut(s) 122
BsiSI CCGG 1 cut(s) 182
BslFI GGGAC 2 cut(s) 205, 270
BslI CCNNNNNNNGG 3 cut(s) 118, 188, 281
BsmAI GTCTC 1 cut(s) 113
BsmFI GGGAC 2 cut(s) 205, 270
BsmI GAATGC 1 cut(s) 43
BsnI GGCC 1 cut(s) 122
Bso31I GGTCTC 1 cut(s) 113
BspACI CCGC 1 cut(s) 15
BspANI GGCC 1 cut(s) 122
BspTNI GGTCTC 1 cut(s) 113
BssECI CCNNGG 1 cut(s) 116
BssSI CACGAG 1 cut(s) 74
BssT1I CCWWGG 1 cut(s) 116
Bst2BI CACGAG 1 cut(s) 74
BstMAI GTCTC 1 cut(s) 113
BstNSI RCATGY 1 cut(s) 198
BsuRI GGCC 1 cut(s) 122
BtsIMutI CAGTG 1 cut(s) 32
Cfr13I GGNCC 1 cut(s) 120
CspCI CAANNNNNGTGG 2 cut(s) 352, 387
CviAII CATG 1 cut(s) 195
CviJI RGCY 4 cut(s) 18, 122, 222, 239
CviKI_1 RGCY 4 cut(s) 18, 122, 222, 239
Eco130I CCWWGG 1 cut(s) 116
Eco31I GGTCTC 1 cut(s) 113
EcoT14I CCWWGG 1 cut(s) 116
EcoT22I ATGCAT 2 cut(s) 6, 43
ErhI CCWWGG 1 cut(s) 116
FaeI CATG 1 cut(s) 198
FaiI YATR 6 cut(s) 6, 125, 134, 196, 329, 356
FaqI GGGAC 2 cut(s) 205, 270
FatI CATG 1 cut(s) 194
Fnu4HI GCNGC 1 cut(s) 16
Fsp4HI GCNGC 1 cut(s) 16
FspBI CTAG 1 cut(s) 117
GluI GCNGC 1 cut(s) 16
HaeIII GGCC 1 cut(s) 122
HapII CCGG 1 cut(s) 182
Hin1II CATG 1 cut(s) 198
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HindIII AAGCTT 1 cut(s) 237
HinfI GANTC 2 cut(s) 101, 295
HpaI GTTAAC 1 cut(s) 307
HpaII CCGG 1 cut(s) 182
HphI GGTGA 1 cut(s) 41
Hpy166II GTNNAC 2 cut(s) 200, 307
Hpy188I TCNGA 4 cut(s) 158, 294, 318, 389
Hpy188III TCNNGA 1 cut(s) 76
Hpy8I GTNNAC 2 cut(s) 200, 307
HpyAV CCTTC 1 cut(s) 316
HpyCH4V TGCA 2 cut(s) 4, 41
Hsp92II CATG 1 cut(s) 198
KspAI GTTAAC 1 cut(s) 307
LmnI GCTCC 1 cut(s) 344
LpnPI CCDG 6 cut(s) 4, 90, 129, 173, 195, 236
MaeI CTAG 1 cut(s) 117
MaeIII GTNAC 1 cut(s) 209
MboII GAAGA 1 cut(s) 331
MluCI AATT 3 cut(s) 151, 159, 285
MmeI TCCRAC 2 cut(s) 136, 272
MnlI CCTC 2 cut(s) 247, 293
Mph1103I ATGCAT 2 cut(s) 6, 43
MseI TTAA 1 cut(s) 306
MspI CCGG 1 cut(s) 182
Mva1269I GAATGC 1 cut(s) 43
NlaIII CATG 1 cut(s) 198
NsiI ATGCAT 2 cut(s) 6, 43
NspI RCATGY 1 cut(s) 198
PciI ACATGT 1 cut(s) 194
PctI GAATGC 1 cut(s) 43
PfeI GAWTC 2 cut(s) 101, 295
PkrI GCNGC 1 cut(s) 17
PscI ACATGT 1 cut(s) 194
PspPI GGNCC 1 cut(s) 120
SaqAI TTAA 1 cut(s) 306
SatI GCNGC 1 cut(s) 16
Sau96I GGNCC 1 cut(s) 120
SetI ASST 3 cut(s) 109, 118, 241
SmlI CTYRAG 1 cut(s) 251
SmoI CTYRAG 1 cut(s) 251
Sse9I AATT 3 cut(s) 151, 159, 285
SsiI CCGC 1 cut(s) 15
SspMI CTAG 1 cut(s) 117
StyI CCWWGG 1 cut(s) 116
TaqI TCGA 1 cut(s) 298
TasI AATT 3 cut(s) 151, 159, 285
TauI GCSGC 1 cut(s) 18
TfiI GAWTC 2 cut(s) 101, 295
Tru1I TTAA 1 cut(s) 306
Tru9I TTAA 1 cut(s) 306
TscAI CASTG 1 cut(s) 39
TspDTI ATGAA 2 cut(s) 72, 344
TspRI CASTG 1 cut(s) 39
XapI RAATTY 2 cut(s) 151, 159
XceI RCATGY 1 cut(s) 198
XmaJI CCTAGG 1 cut(s) 116
XspI CTAG 1 cut(s) 117
Zsp2I ATGCAT 2 cut(s) 6, 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.