Rh6BG462400

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0005079
Physical Location & Seq
Forward (+)
67257196 .. 67260207
3012 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG462400.1

Sequence Viewer

Length: 738 bp
ATGGAAGACTCGGCCGCCGGCAAGCCCAAGAAGTCCGGCGCTCGCCAGTGCTGCATCTGTAACCAGAAAAGAGCTGCTCTCAAGAGGCCCAAAACCCTAGAACAGATTTGCAGGGAGTGTTTCTATGAGGTATTTGAGGAGGAGATACATCAGGTGATTGTTGAAAACCAGTTGTTTAAGCCCGGTGAACGCATTGCCATTGGTGCCTCCGGTGGAAAAGATTCCACTGTTCTTGCCTATGTATTATCTGAATTGAATAAGCGGCACAATTATGGGTTGGATCTCTTCCTGTTGTCTGTGGATGAGGGCATTACAGGCTACAGGGATGATTCTCTTGAAACCGTCAAAAGAAATGAAATGCAGTATGGATTGCCGTTAAAGATTGTGTCGTACAAGGATTTGTATGGGTGGACGATGGATGAAATAGTAAAGATGATTGGTCTCAAGAATAATTGCACATTTTGTGGTGTTTTTCGTCGTCAGGCCCTTGATCGCGGTGCTGCATTGTTGAAAGTAGACAAGCTTGCTACTGGACATAATGCCGATGATATAGCTGAAACAGTTCTATTGAACATATTACGTGGTGATATTGCTAGATTGAGTAGATGCACTTCAATTGTCACTGGTGAAGATGGACCAATTCCAAGATGCAAACCTTTTAAATACACCTATGAGAAGGAAATTGTTATGTACGCATACTTCAAGAGGCTGGATTACTTCTCCACTGAATGTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04122 map04122
Pfam Domains
Protein Families
ATP_bind_3 zn-ribbon_14

Protein Analysis

245

Amino Acids

27.79

Weight (kDa)

7.96

Isoelectric Point (pI)

35.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP_bind_3 PF01171 65 - 245 5.2e-15 PP-loop domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 203
AccI GTMKAC 1 cut(s) 516
AccII CGCG 1 cut(s) 495
AciI CCGC 3 cut(s) 15, 262, 495
AclWI GGATC 1 cut(s) 288
AcoI YGGCCR 1 cut(s) 12
AfaI GTAC 2 cut(s) 392, 692
AgsI TTSAA 7 cut(s) 164, 256, 338, 511, 571, 615, 703
AluBI AGCT 3 cut(s) 74, 523, 554
AluI AGCT 3 cut(s) 74, 523, 554
Alw26I GTCTC 1 cut(s) 446
AlwI GGATC 1 cut(s) 288
AoxI GGCC 3 cut(s) 12, 86, 483
ApeKI GCWGC 3 cut(s) 51, 74, 500
Asp700I GAANNNNTTC 2 cut(s) 220, 561
AspLEI GCGC 1 cut(s) 41
AspS9I GGNCC 3 cut(s) 87, 484, 635
AsuC2I CCSGG 1 cut(s) 183
AsuHPI GGTGA 4 cut(s) 166, 197, 596, 638
AvaII GGWCC 1 cut(s) 635
BanI GGYRCC 1 cut(s) 203
BarI GAAGNNNNNNTAC 4 cut(s) 595, 627, 683, 715
BbsI GAAGAC 1 cut(s) 12
BbvI GCAGC 3 cut(s) 38, 61, 487
BccI CCATC 2 cut(s) 409, 626
BceAI ACGGC 1 cut(s) 358
BcnI CCSGG 1 cut(s) 183
BcoDI GTCTC 1 cut(s) 446
BfaI CTAG 2 cut(s) 98, 594
BfmI CTRYAG 1 cut(s) 319
BfoI RGCGCY 1 cut(s) 42
BisI GCNGC 5 cut(s) 15, 52, 75, 263, 501
BlsI GCNGC 5 cut(s) 16, 53, 76, 264, 502
Bme1390I CCNGG 1 cut(s) 183
Bme18I GGWCC 1 cut(s) 635
BmgT120I GGNCC 3 cut(s) 87, 484, 635
BmiI GGNNCC 1 cut(s) 205
BmrFI CCNGG 1 cut(s) 183
BmsI GCATC 3 cut(s) 63, 596, 638
BpiI GAAGAC 1 cut(s) 12
BplI GAGNNNNNCTC 2 cut(s) 63, 95
BpuEI CTTGAG 2 cut(s) 65, 428
BpuMI CCSGG 1 cut(s) 183
BsaAI YACGTR 1 cut(s) 581
BsaI GGTCTC 1 cut(s) 446
BsaWI WCCGGW 1 cut(s) 209
Bse118I RCCGGY 1 cut(s) 17
Bse1I ACTGG 4 cut(s) 46, 169, 535, 628
Bse3DI GCAATG 1 cut(s) 192
BseGI GGATG 3 cut(s) 307, 331, 424
BseMI GCAATG 1 cut(s) 192
BseNI ACTGG 4 cut(s) 46, 169, 535, 628
BseRI GAGGAG 2 cut(s) 152, 155
BseX3I CGGCCG 1 cut(s) 12
BseXI GCAGC 3 cut(s) 38, 61, 487
Bsh1236I CGCG 1 cut(s) 495
Bsh1285I CGRYCG 1 cut(s) 15
BshFI GGCC 3 cut(s) 14, 88, 485
BshNI GGYRCC 1 cut(s) 203
BsiEI CGRYCG 1 cut(s) 15
BsiSI CCGG 4 cut(s) 18, 36, 183, 210
BsmAI GTCTC 1 cut(s) 446
BsnI GGCC 3 cut(s) 14, 88, 485
Bso31I GGTCTC 1 cut(s) 446
Bsp143I GATC 2 cut(s) 280, 490
BspACI CCGC 3 cut(s) 15, 262, 495
BspANI GGCC 3 cut(s) 14, 88, 485
BspFNI CGCG 1 cut(s) 495
BspLI GGNNCC 1 cut(s) 205
BspPI GGATC 1 cut(s) 288
BspT107I GGYRCC 1 cut(s) 203
BspTNI GGTCTC 1 cut(s) 446
BsrDI GCAATG 1 cut(s) 192
BsrFI RCCGGY 1 cut(s) 17
BsrI ACTGG 4 cut(s) 46, 169, 535, 628
BssAI RCCGGY 1 cut(s) 17
BssMI GATC 2 cut(s) 280, 490
Bst4CI ACNGT 3 cut(s) 229, 343, 562
Bst6I CTCTTC 1 cut(s) 290
BstBAI YACGTR 1 cut(s) 581
BstC8I GCNNGC 4 cut(s) 19, 23, 43, 525
BstF5I GGATG 3 cut(s) 307, 331, 424
BstFNI CGCG 1 cut(s) 495
BstH2I RGCGCY 1 cut(s) 42
BstHHI GCGC 1 cut(s) 41
BstKTI GATC 2 cut(s) 283, 493
BstMAI GTCTC 1 cut(s) 446
BstMBI GATC 2 cut(s) 280, 490
BstMCI CGRYCG 1 cut(s) 15
BstMWI GCNNNNNNNGC 3 cut(s) 51, 203, 315
BstSCI CCNGG 1 cut(s) 181
BstSFI CTRYAG 1 cut(s) 319
BstUI CGCG 1 cut(s) 495
BstV1I GCAGC 3 cut(s) 38, 61, 487
BstV2I GAAGAC 1 cut(s) 12
BstX2I RGATCY 1 cut(s) 280
BstYI RGATCY 1 cut(s) 280
BstZI CGGCCG 1 cut(s) 12
BsuRI GGCC 3 cut(s) 14, 88, 485
BtsCI GGATG 3 cut(s) 307, 331, 424
BtsIMutI CAGTG 4 cut(s) 53, 225, 621, 723
Cac8I GCNNGC 4 cut(s) 19, 23, 43, 525
CfoI GCGC 1 cut(s) 41
Cfr10I RCCGGY 1 cut(s) 17
Cfr13I GGNCC 3 cut(s) 87, 484, 635
Csp6I GTAC 2 cut(s) 391, 691
CspCI CAANNNNNGTGG 2 cut(s) 214, 249
CviQI GTAC 2 cut(s) 391, 691
DpnI GATC 2 cut(s) 282, 492
DpnII GATC 2 cut(s) 280, 490
DraI TTTAAA 1 cut(s) 661
EaeI YGGCCR 1 cut(s) 12
EagI CGGCCG 1 cut(s) 12
Eam1104I CTCTTC 1 cut(s) 290
EarI CTCTTC 1 cut(s) 290
EclXI CGGCCG 1 cut(s) 12
Eco31I GGTCTC 1 cut(s) 446
Eco47I GGWCC 1 cut(s) 635
Eco52I CGGCCG 1 cut(s) 12
EcoO109I RGGNCCY 1 cut(s) 484
FblI GTMKAC 1 cut(s) 516
Fnu4HI GCNGC 5 cut(s) 15, 52, 75, 263, 501
FokI GGATG 3 cut(s) 314, 338, 431
Fsp4HI GCNGC 5 cut(s) 15, 52, 75, 263, 501
FspBI CTAG 2 cut(s) 98, 594
GlaI GCGC 1 cut(s) 40
GluI GCNGC 5 cut(s) 15, 52, 75, 263, 501
HaeII RGCGCY 1 cut(s) 42
HaeIII GGCC 3 cut(s) 14, 88, 485
HapII CCGG 4 cut(s) 18, 36, 183, 210
HhaI GCGC 1 cut(s) 41
Hin6I GCGC 1 cut(s) 39
HinP1I GCGC 1 cut(s) 39
HindIII AAGCTT 1 cut(s) 521
HinfI GANTC 3 cut(s) 8, 221, 329
HpaII CCGG 4 cut(s) 18, 36, 183, 210
HphI GGTGA 4 cut(s) 166, 197, 596, 638
Hpy166II GTNNAC 3 cut(s) 188, 411, 517
Hpy188I TCNGA 1 cut(s) 250
Hpy188III TCNNGA 4 cut(s) 82, 335, 445, 703
Hpy8I GTNNAC 3 cut(s) 188, 411, 517
Hpy99I CGWCG 1 cut(s) 480
HpyAV CCTTC 1 cut(s) 670
HpyCH4III ACNGT 3 cut(s) 229, 343, 562
HpyCH4IV ACGT 1 cut(s) 580
HpyCH4V TGCA 7 cut(s) 54, 111, 361, 456, 503, 609, 651
HpyF10VI GCNNNNNNNGC 3 cut(s) 51, 203, 315
HpySE526I ACGT 1 cut(s) 580
HspAI GCGC 1 cut(s) 39
KroI GCCGGC 1 cut(s) 17
KroNI GCCGGC 1 cut(s) 19
Kzo9I GATC 2 cut(s) 280, 490
Lsp1109I GCAGC 3 cut(s) 38, 61, 487
LweI GCATC 3 cut(s) 63, 596, 638
MaeI CTAG 2 cut(s) 98, 594
MaeII ACGT 1 cut(s) 580
MaeIII GTNAC 2 cut(s) 59, 619
MalI GATC 2 cut(s) 282, 492
MboI GATC 2 cut(s) 280, 490
MboII GAAGA 3 cut(s) 17, 277, 641
MfeI CAATTG 1 cut(s) 615
MflI RGATCY 1 cut(s) 280
MluCI AATT 7 cut(s) 251, 268, 451, 615, 639, 681, 733
MlyI GAGTC 1 cut(s) 2
MmeI TCCRAC 1 cut(s) 258
MnlI CCTC 7 cut(s) 78, 121, 130, 133, 217, 298, 699
MroNI GCCGGC 1 cut(s) 17
MroXI GAANNNNTTC 2 cut(s) 220, 561
MseI TTAA 4 cut(s) 177, 377, 660, 736
MslI CAYNNNNRTG 1 cut(s) 270
MspI CCGG 4 cut(s) 18, 36, 183, 210
MspR9I CCNGG 1 cut(s) 183
MunI CAATTG 1 cut(s) 615
MvnI CGCG 1 cut(s) 495
MwoI GCNNNNNNNGC 3 cut(s) 51, 203, 315
NaeI GCCGGC 1 cut(s) 19
NciI CCSGG 1 cut(s) 183
NdeII GATC 2 cut(s) 280, 490
NgoMIV GCCGGC 1 cut(s) 17
NlaIV GGNNCC 1 cut(s) 205
NmuCI GTSAC 1 cut(s) 619
PdiI GCCGGC 1 cut(s) 19
PdmI GAANNNNTTC 2 cut(s) 220, 561
PfeI GAWTC 2 cut(s) 221, 329
PkrI GCNGC 5 cut(s) 16, 53, 76, 264, 502
PleI GAGTC 1 cut(s) 2
PpsI GAGTC 1 cut(s) 2
Ppu21I YACGTR 1 cut(s) 581
PspN4I GGNNCC 1 cut(s) 205
PspPI GGNCC 3 cut(s) 87, 484, 635
PsuI RGATCY 1 cut(s) 280
RsaI GTAC 2 cut(s) 392, 692
RsaNI GTAC 2 cut(s) 391, 691
RseI CAYNNNNRTG 1 cut(s) 270
SaqAI TTAA 4 cut(s) 177, 377, 660, 736
SatI GCNGC 5 cut(s) 15, 52, 75, 263, 501
Sau3AI GATC 2 cut(s) 280, 490
Sau96I GGNCC 3 cut(s) 87, 484, 635
SchI GAGTC 1 cut(s) 2
ScrFI CCNGG 1 cut(s) 183
SetI ASST 8 cut(s) 76, 132, 156, 525, 556, 583, 658, 671
SfaNI GCATC 3 cut(s) 63, 596, 638
SfcI CTRYAG 1 cut(s) 319
SinI GGWCC 1 cut(s) 635
SmiMI CAYNNNNRTG 1 cut(s) 270
SmlI CTYRAG 2 cut(s) 80, 443
SmoI CTYRAG 2 cut(s) 80, 443
Sse9I AATT 7 cut(s) 251, 268, 451, 615, 639, 681, 733
SsiI CCGC 3 cut(s) 15, 262, 495
SspMI CTAG 2 cut(s) 98, 594
StyD4I CCNGG 1 cut(s) 181
TaaI ACNGT 3 cut(s) 229, 343, 562
TaiI ACGT 1 cut(s) 583
TasI AATT 7 cut(s) 251, 268, 451, 615, 639, 681, 733
TauI GCSGC 2 cut(s) 17, 265
TfiI GAWTC 2 cut(s) 221, 329
Tru1I TTAA 4 cut(s) 177, 377, 660, 736
Tru9I TTAA 4 cut(s) 177, 377, 660, 736
TscAI CASTG 4 cut(s) 53, 232, 628, 730
TseFI GTSAC 1 cut(s) 619
TseI GCWGC 3 cut(s) 51, 74, 500
Tsp45I GTSAC 1 cut(s) 619
TspDTI ATGAA 2 cut(s) 369, 435
TspRI CASTG 4 cut(s) 53, 232, 628, 730
VpaK11BI GGWCC 1 cut(s) 635
XmiI GTMKAC 1 cut(s) 516
XmnI GAANNNNTTC 2 cut(s) 220, 561
XspI CTAG 2 cut(s) 98, 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.