RLG00000011030

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0005079
Physical Location & Seq
Forward (+)
6230266 .. 6232685
2420 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011030

Sequence Viewer

Length: 1056 bp
ATGGAAGACTCGGCTGCCGGCAAGCCCAAGAAGTCCGGCGCTCGCCAGTGCTGCATCTGTAACCAGAAAAGAGCTGCTCTCAAGAGGCCCAAAACCCTAGAACAGATTTGCAGGGAGTGTTTCTATGAGGTATTTGAGGAGGAGATACATCAGGTGATTGTTGAAAACCAGTTGTTTAAGCCCGGTGAACGCATTGCCATTGGTGCCTCCGGTGGAAAAGATTCCACTGTTCTTGCCTATGTATTATCGGAATTGAATAAGCGGCACAATTATGGGTTGGATCTCTTCCTGTTGTCTGTGGATGAGGGCATTACAGGCTACAGGGATGATTCTCTTGAAACCGTCAAAAGAAATGAAATGCAGTATGGATTGCCGTTAAAGATTGTGTCGTACAAGGATCTGTATGGGTGGACGATGGATGAAATAGTAAAGATGATTGGTCTCAAGAATAATTGCACATTTTGTGGTGTTTTTCGTCGTCAGGCCCTTGATCGCGGTGCAGCATTGTTGAAAGTAGACAAGCTTGCTACTGGACATAATGCGGATGATATAGCTGAAACAGTTCTATTGAACATATTACGTGGTGATATTGCAAGATTGAGTAGATGCACTTCAATTGTCACCGGTGAAGATGGACCAATTCCAAGATGCAAACCTTTTAAATACACCTATGAGAAGGAAATTATCTTAGAAGCTGCTGTTGTTAAAATTGTAGGCATTTATTCACCAAATGCTTATCGTGGTTTTGCTCGTGAGTTCATCAAAGATCTGGAAAGAATCAGACCTAGGGCCATACTTGACATAATCAAATCAGGAGAGAATTTCAGAATTTCTACTTCTACAAAAATGCCGGAACAGGGGACATGTGAACGCTGTGGTTACATTTCAAGCCAGAAATGGTGTAAAGCTTGTGTATTGCTTGAGGGACTGAATAGGGGTTTGCCAAAGATGGGAATTGGTCGGAATCGAGGAGTTAACAACAATCAGAAGGATATGAAACAAAGTAATGGAACAAAAAGTATAGAGAGCAAACAATGTGGGACTTTGGACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04122 map04122
Pfam Domains
Protein Families
ATP_bind_3 zn-ribbon_14

Protein Analysis

352

Amino Acids

39.4

Weight (kDa)

8.95

Isoelectric Point (pI)

32.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP_bind_3 PF01171 65 - 228 2.7e-12 PP-loop domain
zn-ribbon_14 PF16503 287 - 317 1.7e-18 Zinc-ribbon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 203
AccI GTMKAC 1 cut(s) 516
AccII CGCG 1 cut(s) 495
AciI CCGC 3 cut(s) 262, 495, 542
AclWI GGATC 2 cut(s) 288, 405
AcsI RAATTY 2 cut(s) 820, 828
AfaI GTAC 1 cut(s) 392
AfiI CCNNNNNNNGG 2 cut(s) 857, 950
AflIII ACRYGT 1 cut(s) 863
AgeI ACCGGT 1 cut(s) 623
AgsI TTSAA 7 cut(s) 164, 256, 338, 511, 571, 615, 888
AluBI AGCT 5 cut(s) 74, 523, 554, 695, 908
AluI AGCT 5 cut(s) 74, 523, 554, 695, 908
Alw26I GTCTC 1 cut(s) 446
AlwI GGATC 2 cut(s) 288, 405
AoxI GGCC 3 cut(s) 86, 483, 789
ApeKI GCWGC 5 cut(s) 14, 51, 74, 500, 695
ApoI RAATTY 2 cut(s) 820, 828
AsiGI ACCGGT 1 cut(s) 623
Asp700I GAANNNNTTC 2 cut(s) 220, 561
AspA2I CCTAGG 1 cut(s) 785
AspLEI GCGC 1 cut(s) 41
AspS9I GGNCC 4 cut(s) 87, 484, 635, 789
AsuC2I CCSGG 1 cut(s) 183
AsuHPI GGTGA 6 cut(s) 166, 197, 596, 613, 638, 717
AvaII GGWCC 1 cut(s) 635
AvrII CCTAGG 1 cut(s) 785
BanI GGYRCC 1 cut(s) 203
BarI GAAGNNNNNNTAC 2 cut(s) 595, 627
BauI CACGAG 1 cut(s) 750
BbsI GAAGAC 1 cut(s) 12
BbvI GCAGC 4 cut(s) 38, 61, 512, 682
BccI CCATC 3 cut(s) 409, 626, 943
BceAI ACGGC 1 cut(s) 358
BcnI CCSGG 1 cut(s) 183
BcoDI GTCTC 1 cut(s) 446
BfaI CTAG 2 cut(s) 98, 786
BfmI CTRYAG 1 cut(s) 319
BfoI RGCGCY 1 cut(s) 42
BglII AGATCT 1 cut(s) 766
BisI GCNGC 6 cut(s) 15, 52, 75, 263, 501, 696
BlnI CCTAGG 1 cut(s) 785
BlsI GCNGC 6 cut(s) 16, 53, 76, 264, 502, 697
Bme1390I CCNGG 1 cut(s) 183
Bme18I GGWCC 1 cut(s) 635
BmgT120I GGNCC 4 cut(s) 87, 484, 635, 789
BmiI GGNNCC 1 cut(s) 205
BmrFI CCNGG 1 cut(s) 183
BmsI GCATC 3 cut(s) 63, 596, 638
BpiI GAAGAC 1 cut(s) 12
BplI GAGNNNNNCTC 2 cut(s) 63, 95
BpuEI CTTGAG 3 cut(s) 65, 428, 941
BpuMI CCSGG 1 cut(s) 183
BsaAI YACGTR 1 cut(s) 581
BsaI GGTCTC 1 cut(s) 446
BsaJI CCNNGG 1 cut(s) 785
BsaWI WCCGGW 2 cut(s) 209, 623
Bsc4I CCNNNNNNNGG 2 cut(s) 857, 950
Bse118I RCCGGY 2 cut(s) 17, 623
Bse1I ACTGG 3 cut(s) 46, 169, 535
Bse3DI GCAATG 1 cut(s) 192
BseDI CCNNGG 1 cut(s) 785
BseGI GGATG 4 cut(s) 307, 331, 424, 550
BseLI CCNNNNNNNGG 2 cut(s) 857, 950
BseMI GCAATG 1 cut(s) 192
BseNI ACTGG 3 cut(s) 46, 169, 535
BseRI GAGGAG 3 cut(s) 152, 155, 984
BseXI GCAGC 4 cut(s) 38, 61, 512, 682
BsgI GTGCAG 1 cut(s) 519
Bsh1236I CGCG 1 cut(s) 495
BshFI GGCC 3 cut(s) 88, 485, 791
BshNI GGYRCC 1 cut(s) 203
BshTI ACCGGT 1 cut(s) 623
BsiSI CCGG 6 cut(s) 18, 36, 183, 210, 624, 851
BslFI GGGAC 2 cut(s) 874, 939
BslI CCNNNNNNNGG 2 cut(s) 857, 950
BsmAI GTCTC 1 cut(s) 446
BsmFI GGGAC 2 cut(s) 874, 939
BsnI GGCC 3 cut(s) 88, 485, 791
Bso31I GGTCTC 1 cut(s) 446
Bsp143I GATC 4 cut(s) 280, 397, 490, 766
BspACI CCGC 3 cut(s) 262, 495, 542
BspANI GGCC 3 cut(s) 88, 485, 791
BspFNI CGCG 1 cut(s) 495
BspLI GGNNCC 1 cut(s) 205
BspPI GGATC 2 cut(s) 288, 405
BspT107I GGYRCC 1 cut(s) 203
BspTNI GGTCTC 1 cut(s) 446
BsrDI GCAATG 1 cut(s) 192
BsrFI RCCGGY 2 cut(s) 17, 623
BsrI ACTGG 3 cut(s) 46, 169, 535
BssAI RCCGGY 2 cut(s) 17, 623
BssECI CCNNGG 1 cut(s) 785
BssMI GATC 4 cut(s) 280, 397, 490, 766
BssSI CACGAG 1 cut(s) 750
BssT1I CCWWGG 1 cut(s) 785
Bst2BI CACGAG 1 cut(s) 750
Bst4CI ACNGT 3 cut(s) 229, 343, 562
Bst6I CTCTTC 1 cut(s) 290
BstBAI YACGTR 1 cut(s) 581
BstC8I GCNNGC 4 cut(s) 19, 23, 43, 525
BstDEI CTNAG 1 cut(s) 688
BstF5I GGATG 4 cut(s) 307, 331, 424, 550
BstFNI CGCG 1 cut(s) 495
BstH2I RGCGCY 1 cut(s) 42
BstHHI GCGC 1 cut(s) 41
BstKTI GATC 4 cut(s) 283, 400, 493, 769
BstMAI GTCTC 1 cut(s) 446
BstMBI GATC 4 cut(s) 280, 397, 490, 766
BstMWI GCNNNNNNNGC 3 cut(s) 51, 203, 315
BstNSI RCATGY 1 cut(s) 867
BstSCI CCNGG 1 cut(s) 181
BstSFI CTRYAG 1 cut(s) 319
BstUI CGCG 1 cut(s) 495
BstV1I GCAGC 4 cut(s) 38, 61, 512, 682
BstV2I GAAGAC 1 cut(s) 12
BstX2I RGATCY 3 cut(s) 280, 397, 766
BstYI RGATCY 3 cut(s) 280, 397, 766
BsuRI GGCC 3 cut(s) 88, 485, 791
BtsCI GGATG 4 cut(s) 307, 331, 424, 550
BtsIMutI CAGTG 2 cut(s) 53, 225
Cac8I GCNNGC 4 cut(s) 19, 23, 43, 525
CfoI GCGC 1 cut(s) 41
Cfr10I RCCGGY 2 cut(s) 17, 623
Cfr13I GGNCC 4 cut(s) 87, 484, 635, 789
Csp6I GTAC 1 cut(s) 391
CspAI ACCGGT 1 cut(s) 623
CspCI CAANNNNNGTGG 4 cut(s) 214, 249, 1018, 1053
CviAII CATG 1 cut(s) 864
CviQI GTAC 1 cut(s) 391
DdeI CTNAG 1 cut(s) 688
DpnI GATC 4 cut(s) 282, 399, 492, 768
DpnII GATC 4 cut(s) 280, 397, 490, 766
DraI TTTAAA 1 cut(s) 661
Eam1104I CTCTTC 1 cut(s) 290
EarI CTCTTC 1 cut(s) 290
Eco130I CCWWGG 1 cut(s) 785
Eco31I GGTCTC 1 cut(s) 446
Eco47I GGWCC 1 cut(s) 635
EcoO109I RGGNCCY 1 cut(s) 484
EcoT14I CCWWGG 1 cut(s) 785
ErhI CCWWGG 1 cut(s) 785
FaeI CATG 1 cut(s) 867
FaqI GGGAC 2 cut(s) 874, 939
FatI CATG 1 cut(s) 863
FblI GTMKAC 1 cut(s) 516
Fnu4HI GCNGC 6 cut(s) 15, 52, 75, 263, 501, 696
FokI GGATG 4 cut(s) 314, 338, 431, 557
Fsp4HI GCNGC 6 cut(s) 15, 52, 75, 263, 501, 696
FspBI CTAG 2 cut(s) 98, 786
GlaI GCGC 1 cut(s) 40
GluI GCNGC 6 cut(s) 15, 52, 75, 263, 501, 696
HaeII RGCGCY 1 cut(s) 42
HaeIII GGCC 3 cut(s) 88, 485, 791
HapII CCGG 6 cut(s) 18, 36, 183, 210, 624, 851
HhaI GCGC 1 cut(s) 41
Hin1II CATG 1 cut(s) 867
Hin6I GCGC 1 cut(s) 39
HinP1I GCGC 1 cut(s) 39
HincII GTYRAC 1 cut(s) 976
HindII GTYRAC 1 cut(s) 976
HindIII AAGCTT 2 cut(s) 521, 906
HinfI GANTC 5 cut(s) 8, 221, 329, 777, 964
HpaI GTTAAC 1 cut(s) 976
HpaII CCGG 6 cut(s) 18, 36, 183, 210, 624, 851
HphI GGTGA 6 cut(s) 166, 197, 596, 613, 638, 717
Hpy166II GTNNAC 5 cut(s) 188, 411, 517, 869, 976
Hpy188I TCNGA 6 cut(s) 250, 782, 827, 963, 987, 1055
Hpy188III TCNNGA 6 cut(s) 82, 335, 445, 752, 770, 813
Hpy8I GTNNAC 5 cut(s) 188, 411, 517, 869, 976
Hpy99I CGWCG 1 cut(s) 480
HpyAV CCTTC 2 cut(s) 670, 982
HpyCH4III ACNGT 3 cut(s) 229, 343, 562
HpyCH4IV ACGT 1 cut(s) 580
HpyCH4V TGCA 8 cut(s) 54, 111, 361, 456, 500, 593, 609, 651
HpyF10VI GCNNNNNNNGC 3 cut(s) 51, 203, 315
HpyF3I CTNAG 1 cut(s) 688
HpySE526I ACGT 1 cut(s) 580
Hsp92II CATG 1 cut(s) 867
HspAI GCGC 1 cut(s) 39
KroI GCCGGC 1 cut(s) 17
KroNI GCCGGC 1 cut(s) 19
KspAI GTTAAC 1 cut(s) 976
Kzo9I GATC 4 cut(s) 280, 397, 490, 766
Lsp1109I GCAGC 4 cut(s) 38, 61, 512, 682
LweI GCATC 3 cut(s) 63, 596, 638
MaeI CTAG 2 cut(s) 98, 786
MaeII ACGT 1 cut(s) 580
MaeIII GTNAC 3 cut(s) 59, 619, 878
MalI GATC 4 cut(s) 282, 399, 492, 768
MboI GATC 4 cut(s) 280, 397, 490, 766
MboII GAAGA 3 cut(s) 17, 277, 641
MfeI CAATTG 1 cut(s) 615
MflI RGATCY 3 cut(s) 280, 397, 766
MlyI GAGTC 1 cut(s) 2
MmeI TCCRAC 2 cut(s) 258, 941
MnlI CCTC 8 cut(s) 78, 121, 130, 133, 217, 298, 916, 962
MroNI GCCGGC 1 cut(s) 17
MroXI GAANNNNTTC 2 cut(s) 220, 561
MseI TTAA 5 cut(s) 177, 377, 660, 705, 975
MslI CAYNNNNRTG 1 cut(s) 270
MspI CCGG 6 cut(s) 18, 36, 183, 210, 624, 851
MspR9I CCNGG 1 cut(s) 183
MunI CAATTG 1 cut(s) 615
MvnI CGCG 1 cut(s) 495
MwoI GCNNNNNNNGC 3 cut(s) 51, 203, 315
NaeI GCCGGC 1 cut(s) 19
NciI CCSGG 1 cut(s) 183
NdeII GATC 4 cut(s) 280, 397, 490, 766
NgoMIV GCCGGC 1 cut(s) 17
NlaIII CATG 1 cut(s) 867
NlaIV GGNNCC 1 cut(s) 205
NmuCI GTSAC 1 cut(s) 619
NspI RCATGY 1 cut(s) 867
PciI ACATGT 1 cut(s) 863
PdiI GCCGGC 1 cut(s) 19
PdmI GAANNNNTTC 2 cut(s) 220, 561
PfeI GAWTC 4 cut(s) 221, 329, 777, 964
PinAI ACCGGT 1 cut(s) 623
PkrI GCNGC 6 cut(s) 16, 53, 76, 264, 502, 697
PleI GAGTC 1 cut(s) 2
PpsI GAGTC 1 cut(s) 2
Ppu21I YACGTR 1 cut(s) 581
PscI ACATGT 1 cut(s) 863
PspN4I GGNNCC 1 cut(s) 205
PspPI GGNCC 4 cut(s) 87, 484, 635, 789
PsuI RGATCY 3 cut(s) 280, 397, 766
RsaI GTAC 1 cut(s) 392
RsaNI GTAC 1 cut(s) 391
RseI CAYNNNNRTG 1 cut(s) 270
SaqAI TTAA 5 cut(s) 177, 377, 660, 705, 975
SatI GCNGC 6 cut(s) 15, 52, 75, 263, 501, 696
Sau3AI GATC 4 cut(s) 280, 397, 490, 766
Sau96I GGNCC 4 cut(s) 87, 484, 635, 789
SchI GAGTC 1 cut(s) 2
ScrFI CCNGG 1 cut(s) 183
SfaNI GCATC 3 cut(s) 63, 596, 638
SfcI CTRYAG 1 cut(s) 319
SgrAI CRCCGGYG 1 cut(s) 623
SinI GGWCC 1 cut(s) 635
SmiMI CAYNNNNRTG 1 cut(s) 270
SmlI CTYRAG 3 cut(s) 80, 443, 920
SmoI CTYRAG 3 cut(s) 80, 443, 920
SsiI CCGC 3 cut(s) 262, 495, 542
SspMI CTAG 2 cut(s) 98, 786
StyD4I CCNGG 1 cut(s) 181
StyI CCWWGG 1 cut(s) 785
TaaI ACNGT 3 cut(s) 229, 343, 562
TaiI ACGT 1 cut(s) 583
TaqI TCGA 1 cut(s) 967
TauI GCSGC 1 cut(s) 265
TfiI GAWTC 4 cut(s) 221, 329, 777, 964
Tru1I TTAA 5 cut(s) 177, 377, 660, 705, 975
Tru9I TTAA 5 cut(s) 177, 377, 660, 705, 975
TscAI CASTG 2 cut(s) 53, 232
TseFI GTSAC 1 cut(s) 619
TseI GCWGC 5 cut(s) 14, 51, 74, 500, 695
Tsp45I GTSAC 1 cut(s) 619
TspDTI ATGAA 4 cut(s) 369, 435, 748, 1010
TspRI CASTG 2 cut(s) 53, 232
VpaK11BI GGWCC 1 cut(s) 635
XapI RAATTY 2 cut(s) 820, 828
XceI RCATGY 1 cut(s) 867
XmaJI CCTAGG 1 cut(s) 785
XmiI GTMKAC 1 cut(s) 516
XmnI GAANNNNTTC 2 cut(s) 220, 561
XspI CTAG 2 cut(s) 98, 786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.