Rorug06G0326200

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0005079
Physical Location & Seq
Forward (+)
47797944 .. 47799168
1225 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0326200.1

Sequence Viewer

Length: 510 bp
ATGGGGACTCTCTCTCTCTTCCCTACTTCTCTCTATGGTCCTCTGCGAACCCATCTCTCATCTTCAAAAACCCATCTCTGCAACTCCATTTTCTTGCCACACGTCTCTGTAACTCATCCTCAACAAAGACGTTTTCGCAGCAAGAATGTTGTTTTCGGTAGCAAATCAGTTGAACCGAAGGAGTCCCAGTTCTTTGATGAAGATGGAGCTGTGGATGACATGGATGGCTACATGAACTACCTTTCTCTTGAATATGACTCCGTTTGGGACACTAAACCTTCCTGGTGCCAACCATGGACAATTACACTGACCGGAGTGCTAGTGATTGCGTTTAGCTGGCTGATTTTGCACTCAGTATTAGCCACAGGCGTTGTAGTCTTCCTAATTGGCTTATGGTGGTACATCTTTCTGTATTCATACCCAAAGGCGTATGCAGATATGATTGCCGAGCGGAGGAAGAGGGTGTCCAACGGTGTTGAAGACACCTTTGGAATGGGGGAGACGCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04122 map04122
Pfam Domains
Protein Families
ATP_bind_3 zn-ribbon_14

Protein Analysis

169

Amino Acids

19.33

Weight (kDa)

6.5

Isoelectric Point (pI)

38.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6737 PF20522 87 - 143 4e-27 Domain of unknown function (DUF6737)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 285
AccBSI CCGCTC 1 cut(s) 451
AccII CGCG 1 cut(s) 505
AciI CCGC 2 cut(s) 451, 505
AfaI GTAC 1 cut(s) 401
AfiI CCNNNNNNNGG 1 cut(s) 453
AflIII ACRYGT 1 cut(s) 100
AgsI TTSAA 4 cut(s) 66, 173, 251, 479
AjiI CACGTC 1 cut(s) 103
AjnI CCWGG 1 cut(s) 281
AjuI GAANNNNNNNTTGG 2 cut(s) 471, 503
AloI GAACNNNNNNTCC 2 cut(s) 173, 205
AluBI AGCT 2 cut(s) 209, 336
AluI AGCT 2 cut(s) 209, 336
Alw26I GTCTC 2 cut(s) 109, 494
ApeKI GCWGC 1 cut(s) 138
AspS9I GGNCC 1 cut(s) 38
AvaII GGWCC 1 cut(s) 38
BanI GGYRCC 1 cut(s) 285
BbsI GAAGAC 2 cut(s) 370, 486
BbvI GCAGC 1 cut(s) 150
BccI CCATC 4 cut(s) 60, 81, 197, 218
BciT130I CCWGG 1 cut(s) 283
BcoDI GTCTC 2 cut(s) 109, 494
BfaI CTAG 1 cut(s) 320
BisI GCNGC 1 cut(s) 139
BlsI GCNGC 1 cut(s) 140
Bme1390I CCNGG 1 cut(s) 283
Bme18I GGWCC 1 cut(s) 38
BmgBI CACGTC 1 cut(s) 103
BmgT120I GGNCC 1 cut(s) 38
BmiI GGNNCC 1 cut(s) 287
BmrFI CCNGG 1 cut(s) 283
BmrI ACTGGG 1 cut(s) 181
BmuI ACTGGG 1 cut(s) 181
BpiI GAAGAC 2 cut(s) 370, 486
BsaJI CCNNGG 1 cut(s) 293
BsaWI WCCGGW 1 cut(s) 311
BsaXI ACNNNNNCTCC 5 cut(s) 173, 203, 306, 336, 491
Bsc4I CCNNNNNNNGG 1 cut(s) 453
Bse1I ACTGG 1 cut(s) 187
BseBI CCWGG 1 cut(s) 283
BseDI CCNNGG 1 cut(s) 293
BseGI GGATG 3 cut(s) 115, 220, 229
BseLI CCNNNNNNNGG 1 cut(s) 453
BseMII CTCAG 1 cut(s) 366
BseNI ACTGG 1 cut(s) 187
BseXI GCAGC 1 cut(s) 150
Bsh1236I CGCG 1 cut(s) 505
BshNI GGYRCC 1 cut(s) 285
BsiSI CCGG 1 cut(s) 312
BslFI GGGAC 3 cut(s) 19, 169, 281
BslI CCNNNNNNNGG 1 cut(s) 453
BsmAI GTCTC 2 cut(s) 109, 494
BsmBI CGTCTC 2 cut(s) 109, 494
BsmFI GGGAC 3 cut(s) 19, 169, 281
Bsp19I CCATGG 1 cut(s) 293
BspACI CCGC 2 cut(s) 451, 505
BspCNI CTCAG 1 cut(s) 365
BspFNI CGCG 1 cut(s) 505
BspLI GGNNCC 1 cut(s) 287
BspT107I GGYRCC 1 cut(s) 285
BsrBI CCGCTC 1 cut(s) 451
BsrI ACTGG 1 cut(s) 187
BssECI CCNNGG 1 cut(s) 293
BssT1I CCWWGG 1 cut(s) 293
Bst2UI CCWGG 1 cut(s) 283
Bst4CI ACNGT 1 cut(s) 473
Bst6I CTCTTC 2 cut(s) 23, 452
BstC8I GCNNGC 1 cut(s) 338
BstDEI CTNAG 1 cut(s) 352
BstDSI CCRYGG 1 cut(s) 293
BstF5I GGATG 3 cut(s) 115, 220, 229
BstFNI CGCG 1 cut(s) 505
BstMAI GTCTC 2 cut(s) 109, 494
BstMWI GCNNNNNNNGC 1 cut(s) 346
BstNI CCWGG 1 cut(s) 283
BstSCI CCNGG 1 cut(s) 281
BstUI CGCG 1 cut(s) 505
BstV1I GCAGC 1 cut(s) 150
BstV2I GAAGAC 2 cut(s) 370, 486
BtgI CCRYGG 1 cut(s) 293
BtrI CACGTC 1 cut(s) 103
BtsCI GGATG 3 cut(s) 115, 220, 229
BtsIMutI CAGTG 1 cut(s) 305
Cac8I GCNNGC 1 cut(s) 338
Cfr13I GGNCC 1 cut(s) 38
Csp6I GTAC 1 cut(s) 400
CspCI CAANNNNNGTGG 2 cut(s) 352, 387
CviAII CATG 3 cut(s) 220, 232, 294
CviJI RGCY 6 cut(s) 209, 228, 336, 340, 362, 390
CviKI_1 RGCY 6 cut(s) 209, 228, 336, 340, 362, 390
CviQI GTAC 1 cut(s) 400
DdeI CTNAG 1 cut(s) 352
Eam1104I CTCTTC 2 cut(s) 23, 452
EarI CTCTTC 2 cut(s) 23, 452
Eco130I CCWWGG 1 cut(s) 293
Eco47I GGWCC 1 cut(s) 38
EcoRII CCWGG 1 cut(s) 281
EcoT14I CCWWGG 1 cut(s) 293
ErhI CCWWGG 1 cut(s) 293
Esp3I CGTCTC 2 cut(s) 109, 494
FaeI CATG 3 cut(s) 223, 235, 297
FaiI YATR 9 cut(s) 36, 221, 233, 255, 295, 394, 418, 432, 440
FaqI GGGAC 3 cut(s) 19, 169, 281
FatI CATG 3 cut(s) 219, 231, 293
Fnu4HI GCNGC 1 cut(s) 139
FokI GGATG 3 cut(s) 102, 227, 236
Fsp4HI GCNGC 1 cut(s) 139
FspBI CTAG 1 cut(s) 320
GluI GCNGC 1 cut(s) 139
HapII CCGG 1 cut(s) 312
Hin1II CATG 3 cut(s) 223, 235, 297
HinfI GANTC 3 cut(s) 7, 182, 257
HpaII CCGG 1 cut(s) 312
Hpy188III TCNNGA 1 cut(s) 248
HpyAV CCTTC 2 cut(s) 172, 288
HpyCH4III ACNGT 1 cut(s) 473
HpyCH4IV ACGT 2 cut(s) 102, 130
HpyCH4V TGCA 3 cut(s) 81, 349, 434
HpyF10VI GCNNNNNNNGC 1 cut(s) 346
HpyF3I CTNAG 1 cut(s) 352
HpySE526I ACGT 2 cut(s) 102, 130
Hsp92II CATG 3 cut(s) 223, 235, 297
LmnI GCTCC 1 cut(s) 206
LpnPI CCDG 6 cut(s) 200, 268, 295, 322, 325, 351
Lsp1109I GCAGC 1 cut(s) 150
MaeI CTAG 1 cut(s) 320
MaeII ACGT 2 cut(s) 102, 130
MaeIII GTNAC 1 cut(s) 109
MbiI CCGCTC 1 cut(s) 451
MboII GAAGA 6 cut(s) 10, 54, 212, 370, 469, 491
MluCI AATT 2 cut(s) 300, 384
MlyI GAGTC 2 cut(s) 191, 251
MmeI TCCRAC 1 cut(s) 492
MnlI CCTC 4 cut(s) 51, 129, 447, 453
MspI CCGG 1 cut(s) 312
MspR9I CCNGG 1 cut(s) 283
MvaI CCWGG 1 cut(s) 283
MvnI CGCG 1 cut(s) 505
MwoI GCNNNNNNNGC 1 cut(s) 346
NcoI CCATGG 1 cut(s) 293
NlaIII CATG 3 cut(s) 223, 235, 297
NlaIV GGNNCC 1 cut(s) 287
NmeAIII GCCGAG 1 cut(s) 472
PkrI GCNGC 1 cut(s) 140
PleI GAGTC 2 cut(s) 190, 251
PpsI GAGTC 2 cut(s) 190, 251
Psp6I CCWGG 1 cut(s) 281
PspGI CCWGG 1 cut(s) 281
PspN4I GGNNCC 1 cut(s) 287
PspPI GGNCC 1 cut(s) 38
RsaI GTAC 1 cut(s) 401
RsaNI GTAC 1 cut(s) 400
SatI GCNGC 1 cut(s) 139
Sau96I GGNCC 1 cut(s) 38
SchI GAGTC 2 cut(s) 191, 251
ScrFI CCNGG 1 cut(s) 283
SetI ASST 7 cut(s) 105, 133, 211, 243, 280, 338, 488
SinI GGWCC 1 cut(s) 38
Sse9I AATT 2 cut(s) 300, 384
SsiI CCGC 2 cut(s) 451, 505
SspMI CTAG 1 cut(s) 320
StyD4I CCNGG 1 cut(s) 281
StyI CCWWGG 1 cut(s) 293
TaaI ACNGT 1 cut(s) 473
TaiI ACGT 2 cut(s) 105, 133
TasI AATT 2 cut(s) 300, 384
TscAI CASTG 1 cut(s) 312
TseI GCWGC 1 cut(s) 138
TspDTI ATGAA 3 cut(s) 213, 248, 405
TspGWI ACGGA 1 cut(s) 250
TspRI CASTG 1 cut(s) 312
VpaK11BI GGWCC 1 cut(s) 38
XspI CTAG 1 cut(s) 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.