FvH4_2g37771

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005079
Physical Location & Seq
Forward (+)
27420922 .. 27424721
3800 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g37771.t2

Sequence Viewer

Length: 1149 bp
ATGAAACGCACAGCGTTAACTGAAATCGCGAACTTTCTCTCTCTATCTTTGAGCTCCCAGCAATTTCCGTCGCCAAAAATGGAAGACTCGGCCGCCGGCAAGCCCAAGAAGATTGGGCCGCGGCAGTGCTGCATCTGTAACCAGAAAAGAGCTGCTCTCAAGCGGCCCAAAACCCTAGAACAGATTTGCAGGGAGTGTTTCTATGAGGTGTTTGAGGAGGAGATTCATCAGGTGATTGTAGAGAACCAGTTGTTTAAGCCCGGTGAGCGAATTGCCATTGGTGCCTCTGGTGGAAAAGATTCCACTGTTCTTGCTTATGTATTGTCCGAATTGAATAAGCGGCACAATTATGGGTTGGATCTCTTCCTGTTGTCTGTGGATGAGGGCATTACAGGCTACAGGGATGATTCGCTGGAAACCGTCAAAAGAAATGAAATGCAGTATGGATTGCCGTTAAAGATTGTGTCATACAAGGATTTGTATGGGTGGACGATGGATGAAATAGTAAAGATGATTGGTCTCAAGAACAATTGCACTTTTTGTGGTGTTTTTCGTCGTCAGGCCCTTGATCGCGGTGCTGCATTGTTGAAAGTGGACAAGCTTGCTACTGGACATAATGCCGATGACATAGCTGAAACAGTTCTGTTGAACATATTACGTGGGGATATTGCAAGATTGAGTAGATGTACTTCAATTGTCACCGGTGAAGATGGACCAATTCCAAGATGCAAACCTTTTAAATACACCTATGAGAAGGAGATTGTTATGTATGCATACTTCAAGAGGCTGGATTACTTCTCCACCGAATGTATTTATTCACCAAATGCTTATCGTGGTTTTGCTCGCGAGTTCATTAAAGATTTGGAAAGAATCAGACCTAGGGCCATACTTGATATAATCAAATCAGGGGAGAATTTCAGAATTTCTACTTCTACGAAAATGCCAGAACAGGGAACATGTGAACGCTGTGGTTACATTTCAAGCCAGAAATGGTGTAAGGCTTGTGTTTTGCTTGAGGGACTGAATAGAGGCCTGCCAAAGCTAGGAATTGGTCGGAATCGGGGAGTCCATAACAATCAGAAGGACATAAAACCAAGTAATGGAACAAAAAGTATTGAGAGCAAACAATGTGGGACTTTGGACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04122 map04122
Pfam Domains
Protein Families
ATP_bind_3 zn-ribbon_14

Protein Analysis

383

Amino Acids

43.21

Weight (kDa)

9.01

Isoelectric Point (pI)

37.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP_bind_3 PF01171 91 - 247 2.2e-12 PP-loop domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 281
AccB7I CCANNNNNTGG 1 cut(s) 1100
AccII CGCG 4 cut(s) 29, 121, 573, 846
AciI CCGC 6 cut(s) 93, 119, 121, 163, 340, 573
AclWI GGATC 1 cut(s) 366
AcoI YGGCCR 1 cut(s) 90
AcsI RAATTY 2 cut(s) 913, 921
AfaI GTAC 1 cut(s) 688
AfiI CCNNNNNNNGG 3 cut(s) 950, 1043, 1100
AflIII ACRYGT 1 cut(s) 956
AgeI ACCGGT 1 cut(s) 701
AgsI TTSAA 6 cut(s) 334, 589, 649, 693, 781, 981
AluBI AGCT 5 cut(s) 54, 152, 601, 632, 1042
AluI AGCT 5 cut(s) 54, 152, 601, 632, 1042
Alw21I GWGCWC 1 cut(s) 56
Alw26I GTCTC 1 cut(s) 524
AlwI GGATC 1 cut(s) 366
AoxI GGCC 6 cut(s) 90, 116, 164, 561, 882, 1030
ApeKI GCWGC 3 cut(s) 129, 152, 578
ApoI RAATTY 2 cut(s) 913, 921
AsiGI ACCGGT 1 cut(s) 701
Asp700I GAANNNNTTC 2 cut(s) 298, 639
AspA2I CCTAGG 1 cut(s) 878
AspS9I GGNCC 5 cut(s) 116, 165, 562, 713, 882
AsuC2I CCSGG 1 cut(s) 261
AsuHPI GGTGA 5 cut(s) 244, 275, 691, 716, 810
AvaII GGWCC 1 cut(s) 713
AvrII CCTAGG 1 cut(s) 878
BanI GGYRCC 1 cut(s) 281
BanII GRGCYC 1 cut(s) 56
BarI GAAGNNNNNNTAC 4 cut(s) 673, 705, 761, 793
BbsI GAAGAC 1 cut(s) 90
Bbv12I GWGCWC 1 cut(s) 56
BbvI GCAGC 3 cut(s) 116, 139, 565
BccI CCATC 2 cut(s) 487, 704
BceAI ACGGC 1 cut(s) 436
BcnI CCSGG 1 cut(s) 261
BcoDI GTCTC 1 cut(s) 524
BfaI CTAG 3 cut(s) 176, 879, 1043
BfmI CTRYAG 1 cut(s) 397
BisI GCNGC 8 cut(s) 93, 119, 122, 130, 153, 164, 341, 579
BlnI CCTAGG 1 cut(s) 878
BlsI GCNGC 8 cut(s) 94, 120, 123, 131, 154, 165, 342, 580
Bme1390I CCNGG 1 cut(s) 261
Bme18I GGWCC 1 cut(s) 713
BmgT120I GGNCC 5 cut(s) 116, 165, 562, 713, 882
BmiI GGNNCC 1 cut(s) 283
BmrFI CCNGG 1 cut(s) 261
BmsI GCATC 2 cut(s) 141, 716
BpiI GAAGAC 1 cut(s) 90
BplI GAGNNNNNCTC 2 cut(s) 141, 173
BpuEI CTTGAG 3 cut(s) 143, 506, 1034
BpuMI CCSGG 1 cut(s) 261
BsaAI YACGTR 1 cut(s) 659
BsaI GGTCTC 1 cut(s) 524
BsaJI CCNNGG 2 cut(s) 119, 878
BsaWI WCCGGW 1 cut(s) 701
Bsc4I CCNNNNNNNGG 3 cut(s) 950, 1043, 1100
Bse118I RCCGGY 2 cut(s) 95, 701
Bse1I ACTGG 2 cut(s) 247, 613
BseDI CCNNGG 2 cut(s) 119, 878
BseGI GGATG 3 cut(s) 385, 409, 502
BseLI CCNNNNNNNGG 3 cut(s) 950, 1043, 1100
BseNI ACTGG 2 cut(s) 247, 613
BseRI GAGGAG 2 cut(s) 230, 233
BseX3I CGGCCG 1 cut(s) 90
BseXI GCAGC 3 cut(s) 116, 139, 565
BseYI CCCAGC 1 cut(s) 57
Bsh1236I CGCG 4 cut(s) 29, 121, 573, 846
Bsh1285I CGRYCG 1 cut(s) 93
BshFI GGCC 6 cut(s) 92, 118, 166, 563, 884, 1032
BshNI GGYRCC 1 cut(s) 281
BshTI ACCGGT 1 cut(s) 701
BsiEI CGRYCG 1 cut(s) 93
BsiHKAI GWGCWC 1 cut(s) 56
BsiSI CCGG 3 cut(s) 96, 261, 702
BslFI GGGAC 1 cut(s) 1032
BslI CCNNNNNNNGG 3 cut(s) 950, 1043, 1100
BsmAI GTCTC 1 cut(s) 524
BsmFI GGGAC 1 cut(s) 1032
BsnI GGCC 6 cut(s) 92, 118, 166, 563, 884, 1032
Bso31I GGTCTC 1 cut(s) 524
Bsp1286I GDGCHC 1 cut(s) 56
Bsp143I GATC 2 cut(s) 358, 568
Bsp68I TCGCGA 2 cut(s) 29, 846
BspACI CCGC 6 cut(s) 93, 119, 121, 163, 340, 573
BspANI GGCC 6 cut(s) 92, 118, 166, 563, 884, 1032
BspFNI CGCG 4 cut(s) 29, 121, 573, 846
BspLI GGNNCC 1 cut(s) 283
BspPI GGATC 1 cut(s) 366
BspT107I GGYRCC 1 cut(s) 281
BspTNI GGTCTC 1 cut(s) 524
BsrFI RCCGGY 2 cut(s) 95, 701
BsrI ACTGG 2 cut(s) 247, 613
BssAI RCCGGY 2 cut(s) 95, 701
BssECI CCNNGG 2 cut(s) 119, 878
BssMI GATC 2 cut(s) 358, 568
BssT1I CCWWGG 1 cut(s) 878
Bst4CI ACNGT 3 cut(s) 307, 421, 640
Bst6I CTCTTC 1 cut(s) 368
BstBAI YACGTR 1 cut(s) 659
BstC8I GCNNGC 5 cut(s) 97, 101, 603, 844, 1034
BstDSI CCRYGG 1 cut(s) 119
BstF5I GGATG 3 cut(s) 385, 409, 502
BstFNI CGCG 4 cut(s) 29, 121, 573, 846
BstKTI GATC 2 cut(s) 361, 571
BstMAI GTCTC 1 cut(s) 524
BstMBI GATC 2 cut(s) 358, 568
BstMCI CGRYCG 1 cut(s) 93
BstMWI GCNNNNNNNGC 3 cut(s) 265, 281, 393
BstNSI RCATGY 1 cut(s) 960
BstSCI CCNGG 1 cut(s) 259
BstSFI CTRYAG 1 cut(s) 397
BstUI CGCG 4 cut(s) 29, 121, 573, 846
BstV1I GCAGC 3 cut(s) 116, 139, 565
BstV2I GAAGAC 1 cut(s) 90
BstX2I RGATCY 1 cut(s) 358
BstYI RGATCY 1 cut(s) 358
BstZI CGGCCG 1 cut(s) 90
BsuRI GGCC 6 cut(s) 92, 118, 166, 563, 884, 1032
BtgI CCRYGG 1 cut(s) 119
BtsCI GGATG 3 cut(s) 385, 409, 502
BtsI GCAGTG 1 cut(s) 131
BtsIMutI CAGTG 2 cut(s) 131, 303
BtuMI TCGCGA 2 cut(s) 29, 846
Cac8I GCNNGC 5 cut(s) 97, 101, 603, 844, 1034
Cfr10I RCCGGY 2 cut(s) 95, 701
Cfr13I GGNCC 5 cut(s) 116, 165, 562, 713, 882
Cfr42I CCGCGG 1 cut(s) 122
Csp6I GTAC 1 cut(s) 687
CspAI ACCGGT 1 cut(s) 701
CspCI CAANNNNNGTGG 4 cut(s) 292, 327, 1111, 1146
CviAII CATG 1 cut(s) 957
CviQI GTAC 1 cut(s) 687
DpnI GATC 2 cut(s) 360, 570
DpnII GATC 2 cut(s) 358, 568
DraI TTTAAA 1 cut(s) 739
EaeI YGGCCR 1 cut(s) 90
EagI CGGCCG 1 cut(s) 90
Eam1104I CTCTTC 1 cut(s) 368
EarI CTCTTC 1 cut(s) 368
Ecl136II GAGCTC 1 cut(s) 54
EclXI CGGCCG 1 cut(s) 90
Eco130I CCWWGG 1 cut(s) 878
Eco147I AGGCCT 1 cut(s) 1032
Eco24I GRGCYC 1 cut(s) 56
Eco31I GGTCTC 1 cut(s) 524
Eco47I GGWCC 1 cut(s) 713
Eco52I CGGCCG 1 cut(s) 90
Eco53kI GAGCTC 1 cut(s) 54
EcoICRI GAGCTC 1 cut(s) 54
EcoO109I RGGNCCY 1 cut(s) 562
EcoT14I CCWWGG 1 cut(s) 878
EcoT22I ATGCAT 1 cut(s) 775
EcoT38I GRGCYC 1 cut(s) 56
ErhI CCWWGG 1 cut(s) 878
FaeI CATG 1 cut(s) 960
FaqI GGGAC 1 cut(s) 1032
FatI CATG 1 cut(s) 956
Fnu4HI GCNGC 8 cut(s) 93, 119, 122, 130, 153, 164, 341, 579
FokI GGATG 3 cut(s) 392, 416, 509
FriOI GRGCYC 1 cut(s) 56
Fsp4HI GCNGC 8 cut(s) 93, 119, 122, 130, 153, 164, 341, 579
FspBI CTAG 3 cut(s) 176, 879, 1043
GluI GCNGC 8 cut(s) 93, 119, 122, 130, 153, 164, 341, 579
GsaI CCCAGC 1 cut(s) 61
HaeIII GGCC 6 cut(s) 92, 118, 166, 563, 884, 1032
HapII CCGG 3 cut(s) 96, 261, 702
Hin1II CATG 1 cut(s) 960
HincII GTYRAC 1 cut(s) 18
HindII GTYRAC 1 cut(s) 18
HindIII AAGCTT 1 cut(s) 599
HinfI GANTC 7 cut(s) 86, 223, 299, 407, 870, 1057, 1065
HpaI GTTAAC 1 cut(s) 18
HpaII CCGG 3 cut(s) 96, 261, 702
HphI GGTGA 5 cut(s) 244, 275, 691, 716, 810
Hpy166II GTNNAC 4 cut(s) 18, 489, 595, 962
Hpy188I TCNGA 6 cut(s) 328, 875, 920, 1056, 1080, 1148
Hpy188III TCNNGA 4 cut(s) 28, 523, 781, 845
Hpy8I GTNNAC 4 cut(s) 18, 489, 595, 962
Hpy99I CGWCG 2 cut(s) 73, 558
HpyAV CCTTC 2 cut(s) 748, 1075
HpyCH4III ACNGT 3 cut(s) 307, 421, 640
HpyCH4IV ACGT 1 cut(s) 658
HpyCH4V TGCA 8 cut(s) 132, 189, 439, 534, 581, 671, 729, 773
HpyF10VI GCNNNNNNNGC 3 cut(s) 265, 281, 393
HpySE526I ACGT 1 cut(s) 658
Hsp92II CATG 1 cut(s) 960
KroI GCCGGC 1 cut(s) 95
KroNI GCCGGC 1 cut(s) 97
KspAI GTTAAC 1 cut(s) 18
KspI CCGCGG 1 cut(s) 122
Kzo9I GATC 2 cut(s) 358, 568
LmnI GCTCC 1 cut(s) 59
Lsp1109I GCAGC 3 cut(s) 116, 139, 565
LweI GCATC 2 cut(s) 141, 716
MaeI CTAG 3 cut(s) 176, 879, 1043
MaeII ACGT 1 cut(s) 658
MaeIII GTNAC 3 cut(s) 137, 697, 971
MalI GATC 2 cut(s) 360, 570
MboI GATC 2 cut(s) 358, 568
MboII GAAGA 4 cut(s) 95, 121, 355, 719
MfeI CAATTG 2 cut(s) 529, 693
MflI RGATCY 1 cut(s) 358
MhlI GDGCHC 1 cut(s) 56
MlyI GAGTC 2 cut(s) 80, 1074
MmeI TCCRAC 2 cut(s) 336, 1034
MnlI CCTC 8 cut(s) 199, 208, 211, 295, 376, 777, 1009, 1022
Mph1103I ATGCAT 1 cut(s) 775
MroNI GCCGGC 1 cut(s) 95
MroXI GAANNNNTTC 2 cut(s) 298, 639
MseI TTAA 5 cut(s) 17, 255, 455, 738, 855
MslI CAYNNNNRTG 1 cut(s) 348
MspA1I CMGCKG 1 cut(s) 121
MspI CCGG 3 cut(s) 96, 261, 702
MspR9I CCNGG 1 cut(s) 261
MunI CAATTG 2 cut(s) 529, 693
MvnI CGCG 4 cut(s) 29, 121, 573, 846
MwoI GCNNNNNNNGC 3 cut(s) 265, 281, 393
NaeI GCCGGC 1 cut(s) 97
NciI CCSGG 1 cut(s) 261
NdeII GATC 2 cut(s) 358, 568
NgoMIV GCCGGC 1 cut(s) 95
NlaIII CATG 1 cut(s) 960
NlaIV GGNNCC 1 cut(s) 283
NmeAIII GCCGAG 1 cut(s) 68
NmuCI GTSAC 1 cut(s) 697
NruI TCGCGA 2 cut(s) 29, 846
NsiI ATGCAT 1 cut(s) 775
NspI RCATGY 1 cut(s) 960
PceI AGGCCT 1 cut(s) 1032
PciI ACATGT 1 cut(s) 956
PdiI GCCGGC 1 cut(s) 97
PdmI GAANNNNTTC 2 cut(s) 298, 639
PfeI GAWTC 5 cut(s) 223, 299, 407, 870, 1057
PflMI CCANNNNNTGG 1 cut(s) 1100
PinAI ACCGGT 1 cut(s) 701
PkrI GCNGC 8 cut(s) 94, 120, 123, 131, 154, 165, 342, 580
PleI GAGTC 2 cut(s) 80, 1073
PpsI GAGTC 2 cut(s) 80, 1073
Ppu21I YACGTR 1 cut(s) 659
PscI ACATGT 1 cut(s) 956
Psp124BI GAGCTC 1 cut(s) 56
PspFI CCCAGC 1 cut(s) 57
PspN4I GGNNCC 1 cut(s) 283
PspPI GGNCC 5 cut(s) 116, 165, 562, 713, 882
PsuI RGATCY 1 cut(s) 358
RruI TCGCGA 2 cut(s) 29, 846
RsaI GTAC 1 cut(s) 688
RsaNI GTAC 1 cut(s) 687
RseI CAYNNNNRTG 1 cut(s) 348
SacI GAGCTC 1 cut(s) 56
SacII CCGCGG 1 cut(s) 122
SaqAI TTAA 5 cut(s) 17, 255, 455, 738, 855
SatI GCNGC 8 cut(s) 93, 119, 122, 130, 153, 164, 341, 579
Sau3AI GATC 2 cut(s) 358, 568
Sau96I GGNCC 5 cut(s) 116, 165, 562, 713, 882
SchI GAGTC 2 cut(s) 80, 1074
ScrFI CCNGG 1 cut(s) 261
SduI GDGCHC 1 cut(s) 56
SfaNI GCATC 2 cut(s) 141, 716
SfcI CTRYAG 1 cut(s) 397
Sfr303I CCGCGG 1 cut(s) 122
SgrAI CRCCGGYG 1 cut(s) 701
SgrBI CCGCGG 1 cut(s) 122
SinI GGWCC 1 cut(s) 713
SmiMI CAYNNNNRTG 1 cut(s) 348
SmlI CTYRAG 3 cut(s) 158, 521, 1013
SmoI CTYRAG 3 cut(s) 158, 521, 1013
SseBI AGGCCT 1 cut(s) 1032
SsiI CCGC 6 cut(s) 93, 119, 121, 163, 340, 573
SspMI CTAG 3 cut(s) 176, 879, 1043
SstI GAGCTC 1 cut(s) 56
StuI AGGCCT 1 cut(s) 1032
StyD4I CCNGG 1 cut(s) 259
StyI CCWWGG 1 cut(s) 878
TaaI ACNGT 3 cut(s) 307, 421, 640
TaiI ACGT 1 cut(s) 661
TatI WGTACW 1 cut(s) 686
TauI GCSGC 5 cut(s) 95, 121, 124, 166, 343
TfiI GAWTC 5 cut(s) 223, 299, 407, 870, 1057
Tru1I TTAA 5 cut(s) 17, 255, 455, 738, 855
Tru9I TTAA 5 cut(s) 17, 255, 455, 738, 855
TscAI CASTG 2 cut(s) 131, 310
TseFI GTSAC 1 cut(s) 697
TseI GCWGC 3 cut(s) 129, 152, 578
Tsp45I GTSAC 1 cut(s) 697
TspDTI ATGAA 5 cut(s) 17, 215, 447, 513, 841
TspGWI ACGGA 1 cut(s) 57
TspRI CASTG 2 cut(s) 131, 310
Van91I CCANNNNNTGG 1 cut(s) 1100
VpaK11BI GGWCC 1 cut(s) 713
XapI RAATTY 2 cut(s) 913, 921
XceI RCATGY 1 cut(s) 960
XmaJI CCTAGG 1 cut(s) 878
XmnI GAANNNNTTC 2 cut(s) 298, 639
XspI CTAG 3 cut(s) 176, 879, 1043
Zsp2I ATGCAT 1 cut(s) 775
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.