AT2G04235

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0005008
Physical Location & Seq
Forward (+)
1449288 .. 1455314
6027 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G04235.2

Sequence Viewer

Length: 3762 bp
ATGGCATCGGAAAAGCCAGAAGATCCGATGAACAACACCGCCGGAATAGGAACAGACGAGGAATCTATAGCGCAACGGCGGAAGCGGCTAAGACGAGTGAGCTTCGCCGACCGGGAAATTACTTCCGTTCACATTTTCAACCGGGACGAAGATTACGAGACGCCGCCCAATACCTCCGCCGCGAAACCCCAAAACGGTGGTGATACATCAGAGCCTGATGAGGATAATAAAGTAATTAGGTTTTTCGGTGAATTATCTGACCGCGAAGATACTGACGGAGATGGTGATGGTGAATACGAACCTATCTTGGATAAATCATTCTTGAGGCCGAAATATTCTCCATCTTCAGGAGGTAGCACTGTTGGTTCCGCTACAAGTGATAACGGTACGCTTCAATTGCTCTGTGAATTTAGGGTTTTGTTCTTTGAATTTTTAGCTGAATCGATTTTGTTTTCGTTACCAGAGGATAATTTCTTCGGTCCTGTGTCTTCCCATTTTATTAATCCTGGAAGATTGTTGGATACTCCTATCTCTGAAGAACACCATGAAATGACGATGGATTCTACGGCGTTTTCGATGCATTTTCGGAGTCTTGCTAGGTCAGAGTCTGGAGATGTAAGGACCCCTACGAGTAGCCATCTTCTAGTAGAAGAGAAAACACCAACTGAGGTTACTTCCAGATCTGATACAGGAAGTGCAATGGTGTTAACAGAACCTAAGAAGCTATTTCCAAAATCACCTGTACCAGTTGATAAAGGAAGTGGTGGTAGAGACTCGAATGATATGAGTATCGTCGGTGAAAATTCTCGGAGATATGATTACGGGTATCTATCTCCTACACTAGCAGCTCTAATGGGAGATGAAAGCAAGGAATTGCTTCCTGAAGACAATACTGTGGAAGCAAGATCACCTATCGATGATTTTTCCTCGTCCCTTCCAAATGGATGCATTCCCATAGGTCTGCAGGAATCAGGAAGCCAACGTTACACAAAAGAGGCTTCCCTTTCCTCGTCAACTATTAGAAGGCAAAGTGCGTTTTTAGTTGGTATGTTGCCTCAGTCTTTATCTTGTGTTACTCCTTCCCCTACACAGGGTGGAAGCTTTATGAGCAGGGAGACTCGGGCACTAGTTGAGAGCTTGTCAACCATTCAGAAAAGCAAATCCAGACTTGGTTTGATTCCACCGTCACCTGGTTCTGCTCTTTCTCAAAGAATTGAGAAATCAAAGCTTCAATTATCCGGACACCGTTTTCTGACTACCCCATCAATTGGGAGGGAAGAAATAGGTGTACTCAGGGATAAGCATGCGGATATACCCATTACCAACTTGGAGGCTTTGTTATCTAAACATGATAATAGAACACCAATTTCTGAAGAGAAAAGCATGCCAGATAAATGCATTAGTGGTGCATTGAGCCATGCTGTTGACACTAGCGATGATAATAGAACACCAGTTCCAGAAGAGAAAGGCATACCAGATCAATGTATTAGTGGTGCATTGAGCCATGCTGTTGACACTAGCGATGATAATAAAACACCAGTTCCAGAAGAGAAAGGCATACCAGATCAATGCAGTAGTGGTGCATTGAACCCTGCCGTTGACACTAGCGATGATAATAGAACACCAGTTCAAGAAAAGAAAGGCTTGCCAGATCAATGCAGTAGTGGTGCATTGAGCCCTGCTGTTGACACTAGCGATGACAGGCCACCAGTTTCTGAAAAGAAAGGCATACCAGATCAACACAGTTGCGGTGCATTGATTCCTGCTGTTGACATTAGCGATGTGTTTGCACGGAGAAGTCCAGAAGGAAATACTAATTCTGAGATAGAAGGTTCACTGCTCTGTAAACAACAACAGAGAAATCAGGCAGCCAGCACCCCTGAGAAGTTTGTGTCATCTCCAACAAATTTGTCAAATGCAACAACGTCAGCATCGGAGAATTTTGTTCCTCTTCAAGATCAAGAGCAGCATAGTAAAGACATCGAAAAATCTGAGACTGGGGATGGAAATGTGACCAAGGAATATGCTAGTAATTGTTCTATGAATACATTATCTGAGAAAGTGGATTCCCTGCTTGCAGAATCTAGCGTATTACTTACCGATACAGGCTTCTTGAATGGCTCTGCTCAACAAAGAGAGAAGGACAGTGTGCGGAACAAAAAGCAAAACAGAACCAATATCAGTGCTGCTCATATCCTTCTGAAAGATAATAATCCCTTTAAAGTCCATTGTGAAACAGAAGTTATCTCAGCTGAAGACTTTACTGCTGTAGCTAAAGAAAACCTTCCTAGTACCTCTGGCTCTTCATCTGTGGACAGAAGCAAGAATGAGGCTTCACATGCTAAGGGACCATCCAGATTGAAAAGAAAAGCAGAAGATGTTGATTGTGCTGCTAGAAATTGTAGCCCCAAAGTCGAGAGAAGTACAAAGTATATTTCGAATTCAGTGATGGAACACCCTGATGGAAACATTGATGCTAACGATTGCCGCAGAGTTCGTGAACAAGTGAACTGGGTAGAAATTCCAGGAAAAGTATCAAAGGAAATCAATCAAATGCTTGCTCCATTGGCAGATAAGCTGAACTCAAGACTGATATGCAAGCTGGAAGATATATTAACACACATGAAAAAGGTTCATTTGTGTGAGATGCTTTGTCTACAAATTCAATCTCAGAAAGTATGTGACCACTTAAGTGGTGCCAAGACAAAGAGACGTGTTGAGAGTAGATCACTACTCTGCAAGTTAGCATACGATAAAGCTAAACTTGAGTTGCTGCACCTCAAGAAGGAGATAATGATGAAAAAGTTTCAAGCAGTGAGCACCGGTGTACAAACATCCGAAACATTGAGGTTGAATTGTGCTAACTTTCTACGTCAACATGGTTTCCGTTCTACTGGCTTGTTAAATCCTGACCAAGCACAAGAGGTTATTATAACTGGCAAACGAGCAGAGATAACACAGGAGATCAAAGAAATTGACTCAAAAATCAAGAACTTGATTCAATGTTTTACGGCTTGCGACACGATGACAGGGCCGCAGCCGGCATATGCAGACACTATTATGATTGCTGAAGAGACCTTGAAAAAGAGAATGAGTTGCAGATCCCTACGTCAGGATATTCTGATTTGGAAAGTTGACAGTTTAGGTGAATGGAACGATTGTCAGAGTATTGTTCTAAACTATAGTGGCGTGTTCAACCAAAGGCTCACACTAAAGCCTGGCCATCCTTCATGTGTACTAGTTTCAAACAGTTTGAGTGATACATTCGTTAAGCATTTCCCAGAGATGAATGTTTCAATTGCGTTTAACTCTATGTTTAACGCTGAAGACTCCCGGAGGTATATTGGTGGTTCCAATACATTGTTGGAAATTACACAGAAAACCAGTTTGCTCCTACACAATCTGCTAGATGTGGCTGAGGAATTTCATTTAGCTCAAATGAATATACCAAATTTGGTCCAAGGAAACTTTGATTCTCCATCAGCGGAGCAGCTACATTTGCAGATTAGCTTTCTTGATTGCACAAACTTGAGAAAACTAAGCGTGATTCTCGATGTGACATGTTTGATCCATGGGAAATATCCTAGTGATGTAGTTCCTTGCGAGTTCCGTAAAGTTTCAGGCACAAAAAGAGACGGTGTTGTGTCGAAACAACTGAAGAAGGAGATAGAATCTACAATTGATGATGTTGGAGTGGGATATCCTCGGATATTGAGACTCTGTCGTTGCATTTCCAAAGCGTTGCAATCAGAAAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1253

Amino Acids

137.91

Weight (kDa)

5.64

Isoelectric Point (pI)

50.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Knl1_RWD_C PF18210 958 - 1112 5.9e-13 Knl1 RWD C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2933
AccB1I GGYRCC 1 cut(s) 2696
AccB7I CCANNNNNTGG 1 cut(s) 1268
AccI GTMKAC 1 cut(s) 2656
AccII CGCG 2 cut(s) 182, 264
AccIII TCCGGA 1 cut(s) 1238
AclI AACGTT 1 cut(s) 982
AclWI GGATC 3 cut(s) 17, 3096, 3562
AcoI YGGCCR 1 cut(s) 3220
AcuI CTGAAG 8 cut(s) 330, 555, 903, 1392, 2274, 3090, 3345, 3677
AcyI GRCGYC 1 cut(s) 161
AdeI CACNNNGTG 1 cut(s) 1094
AfaI GTAC 7 cut(s) 388, 744, 1290, 2293, 2425, 2829, 3237
AfiI CCNNNNNNNGG 8 cut(s) 194, 347, 1090, 1091, 1190, 1268, 2785, 3112
AflII CTTAAG 1 cut(s) 2689
AflIII ACRYGT 2 cut(s) 2713, 3560
AgeI ACCGGT 1 cut(s) 2822
AhlI ACTAGT 2 cut(s) 1126, 3238
AjiI CACGTC 1 cut(s) 2714
AjnI CCWGG 4 cut(s) 505, 1189, 2524, 3217
AleI CACNNNNGTG 1 cut(s) 2691
AloI GAACNNNNNNTCC 2 cut(s) 1438, 1470
Alw21I GWGCWC 1 cut(s) 2822
Alw26I GTCTC 8 cut(s) 152, 765, 1109, 1988, 2704, 3068, 3627, 3709
AlwI GGATC 3 cut(s) 17, 3096, 3562
AlwNI CAGNNNCTG 3 cut(s) 215, 608, 1715
Ama87I CYCGRG 1 cut(s) 1119
Aor13HI TCCGGA 1 cut(s) 1238
AoxI GGCC 4 cut(s) 326, 1703, 3032, 3220
ApeKI GCWGC 8 cut(s) 845, 1868, 1966, 2186, 2390, 2773, 3037, 3490
AseI ATTAAT 1 cut(s) 501
AsiGI ACCGGT 1 cut(s) 2822
Asp700I GAANNNNTTC 2 cut(s) 876, 2283
AspLEI GCGC 1 cut(s) 73
AspS9I GGNCC 5 cut(s) 479, 621, 2348, 3032, 3457
AsuC2I CCSGG 3 cut(s) 113, 143, 3334
AsuHPI GGTGA 9 cut(s) 212, 260, 296, 302, 729, 809, 900, 1179, 3158
AsuII TTCGAA 1 cut(s) 2438
AvaI CYCGRG 1 cut(s) 1119
AvaII GGWCC 4 cut(s) 479, 621, 2348, 3457
BaeGI GKGCMC 1 cut(s) 1126
BaeI ACNNNNGTAYC 2 cut(s) 3252, 3285
BalI TGGCCA 1 cut(s) 3222
BanI GGYRCC 1 cut(s) 2696
BanII GRGCYC 1 cut(s) 1679
BbsI GAAGAC 4 cut(s) 480, 891, 2262, 3333
Bbv12I GWGCWC 1 cut(s) 2822
BbvCI CCTCAGC 1 cut(s) 3417
BbvI GCAGC 8 cut(s) 857, 1880, 1978, 2173, 2377, 2760, 3049, 3502
BceAI ACGGC 4 cut(s) 92, 582, 1580, 3027
BcgI CGANNNNNNTGC 2 cut(s) 1769, 1803
BciT130I CCWGG 4 cut(s) 507, 1191, 2526, 3219
BciVI GTATCC 1 cut(s) 514
BcnI CCSGG 3 cut(s) 113, 143, 3334
BcoDI GTCTC 8 cut(s) 152, 765, 1109, 1988, 2704, 3068, 3627, 3709
BcuI ACTAGT 2 cut(s) 1126, 3238
BfmI CTRYAG 4 cut(s) 66, 962, 2268, 3181
BfrI CTTAAG 1 cut(s) 2689
BfuI GTATCC 1 cut(s) 514
BglII AGATCT 1 cut(s) 680
Bme1390I CCNGG 7 cut(s) 113, 143, 507, 1191, 2526, 3219, 3334
Bme18I GGWCC 4 cut(s) 479, 621, 2348, 3457
BmeT110I CYCGRG 1 cut(s) 1119
BmgBI CACGTC 1 cut(s) 2714
BmgT120I GGNCC 5 cut(s) 479, 621, 2348, 3032, 3457
BmiI GGNNCC 5 cut(s) 367, 623, 2349, 2698, 3352
BmrFI CCNGG 7 cut(s) 113, 143, 507, 1191, 2526, 3219, 3334
BmrI ACTGGG 2 cut(s) 2007, 2521
BmsI GCATC 6 cut(s) 14, 567, 935, 1940, 2464, 2637
BmuI ACTGGG 2 cut(s) 2007, 2521
BpiI GAAGAC 4 cut(s) 480, 891, 2262, 3333
BpmI CTGGAG 1 cut(s) 630
Bpu10I CCTNAGC 2 cut(s) 2343, 3417
Bpu14I TTCGAA 1 cut(s) 2438
BpuEI CTTGAG 5 cut(s) 343, 2569, 2765, 2786, 3550
BpuMI CCSGG 3 cut(s) 113, 143, 3334
Bsa29I ATCGAT 2 cut(s) 443, 915
BsaBI GATNNNNATC 1 cut(s) 2211
BsaHI GRCGYC 1 cut(s) 161
BsaI GGTCTC 1 cut(s) 3068
BsaJI CCNNGG 4 cut(s) 2016, 3460, 3571, 3704
BsaWI WCCGGW 2 cut(s) 1238, 2822
BsaXI ACNNNNNCTCC 2 cut(s) 1322, 1352
Bsc4I CCNNNNNNNGG 8 cut(s) 194, 347, 1090, 1091, 1190, 1268, 2785, 3112
Bse118I RCCGGY 2 cut(s) 2822, 3040
Bse3DI GCAATG 1 cut(s) 705
Bse8I GATNNNNATC 1 cut(s) 2211
BseAI TCCGGA 1 cut(s) 1238
BseBI CCWGG 4 cut(s) 507, 1191, 2526, 3219
BseCI ATCGAT 2 cut(s) 443, 915
BseDI CCNNGG 4 cut(s) 2016, 3460, 3571, 3704
BseGI GGATG 5 cut(s) 950, 2008, 2351, 2834, 3223
BseJI GATNNNNATC 1 cut(s) 2211
BseLI CCNNNNNNNGG 8 cut(s) 194, 347, 1090, 1091, 1190, 1268, 2785, 3112
BseMI GCAATG 1 cut(s) 705
BseSI GKGCMC 1 cut(s) 1126
BseXI GCAGC 8 cut(s) 857, 1880, 1978, 2173, 2377, 2760, 3049, 3502
BsgI GTGCAG 1 cut(s) 2759
Bsh1236I CGCG 2 cut(s) 182, 264
Bsh1285I CGRYCG 1 cut(s) 112
BshFI GGCC 4 cut(s) 328, 1705, 3034, 3222
BshNI GGYRCC 1 cut(s) 2696
BshTI ACCGGT 1 cut(s) 2822
BshVI ATCGAT 2 cut(s) 443, 915
BsiEI CGRYCG 1 cut(s) 112
BsiHKAI GWGCWC 1 cut(s) 2822
BsiHKCI CYCGRG 1 cut(s) 1119
BsiSI CCGG 7 cut(s) 42, 112, 142, 1239, 2823, 3041, 3334
BslFI GGGAC 3 cut(s) 158, 916, 2361
BslI CCNNNNNNNGG 8 cut(s) 194, 347, 1090, 1091, 1190, 1268, 2785, 3112
BsmAI GTCTC 8 cut(s) 152, 765, 1109, 1988, 2704, 3068, 3627, 3709
BsmBI CGTCTC 3 cut(s) 152, 2704, 3627
BsmFI GGGAC 3 cut(s) 158, 916, 2361
BsmI GAATGC 1 cut(s) 948
BsnI GGCC 4 cut(s) 328, 1705, 3034, 3222
Bso31I GGTCTC 1 cut(s) 3068
BsoBI CYCGRG 1 cut(s) 1119
Bsp119I TTCGAA 1 cut(s) 2438
Bsp1286I GDGCHC 3 cut(s) 1126, 1679, 2822
Bsp13I TCCGGA 1 cut(s) 1238
Bsp1407I TGTACA 1 cut(s) 2827
Bsp19I CCATGG 1 cut(s) 3571
BspANI GGCC 4 cut(s) 328, 1705, 3034, 3222
BspDI ATCGAT 2 cut(s) 443, 915
BspEI TCCGGA 1 cut(s) 1238
BspFNI CGCG 2 cut(s) 182, 264
BspLI GGNNCC 5 cut(s) 367, 623, 2349, 2698, 3352
BspMAI CTGCAG 1 cut(s) 966
BspPI GGATC 3 cut(s) 17, 3096, 3562
BspQI GCTCTTC 1 cut(s) 2308
BspT104I TTCGAA 1 cut(s) 2438
BspT107I GGYRCC 1 cut(s) 2696
BspTI CTTAAG 1 cut(s) 2689
BspTNI GGTCTC 1 cut(s) 3068
BsrDI GCAATG 1 cut(s) 705
BsrFI RCCGGY 2 cut(s) 2822, 3040
BsrGI TGTACA 1 cut(s) 2827
BssAI RCCGGY 2 cut(s) 2822, 3040
BssECI CCNNGG 4 cut(s) 2016, 3460, 3571, 3704
BssNI GRCGYC 1 cut(s) 161
BssT1I CCWWGG 3 cut(s) 2016, 3460, 3571
Bst2UI CCWGG 4 cut(s) 507, 1191, 2526, 3219
Bst6I CTCTTC 7 cut(s) 645, 1368, 1455, 1542, 1956, 2308, 3066
BstACI GRCGYC 1 cut(s) 161
BstAFI CTTAAG 1 cut(s) 2689
BstAUI TGTACA 1 cut(s) 2827
BstBI TTCGAA 1 cut(s) 2438
BstC8I GCNNGC 9 cut(s) 1305, 1385, 1646, 1873, 2076, 2559, 2600, 3016, 3042
BstDSI CCRYGG 1 cut(s) 3571
BstENI CCTNNNNNAGG 2 cut(s) 2783, 3110
BstF5I GGATG 5 cut(s) 950, 2008, 2351, 2834, 3223
BstFNI CGCG 2 cut(s) 182, 264
BstHHI GCGC 1 cut(s) 73
BstMAI GTCTC 8 cut(s) 152, 765, 1109, 1988, 2704, 3068, 3627, 3709
BstMCI CGRYCG 1 cut(s) 112
BstMWI GCNNNNNNNGC 6 cut(s) 85, 397, 1107, 2339, 2567, 3499
BstNI CCWGG 4 cut(s) 507, 1191, 2526, 3219
BstNSI RCATGY 4 cut(s) 1307, 1387, 2342, 3564
BstSCI CCNGG 7 cut(s) 111, 141, 505, 1189, 2524, 3217, 3332
BstSFI CTRYAG 4 cut(s) 66, 962, 2268, 3181
BstSLI GKGCMC 1 cut(s) 1126
BstUI CGCG 2 cut(s) 182, 264
BstV1I GCAGC 8 cut(s) 857, 1880, 1978, 2173, 2377, 2760, 3049, 3502
BstV2I GAAGAC 4 cut(s) 480, 891, 2262, 3333
BstX2I RGATCY 3 cut(s) 22, 680, 3101
BstXI CCANNNNNNTGG 2 cut(s) 197, 2693
BstYI RGATCY 3 cut(s) 22, 680, 3101
Bsu15I ATCGAT 2 cut(s) 443, 915
BsuI GTATCC 1 cut(s) 514
BsuRI GGCC 4 cut(s) 328, 1705, 3034, 3222
BsuTUI ATCGAT 2 cut(s) 443, 915
BtgI CCRYGG 1 cut(s) 3571
BtgZI GCGATG 5 cut(s) 1449, 1536, 1623, 1710, 1794
BtrI CACGTC 1 cut(s) 2714
BtsCI GGATG 5 cut(s) 950, 2008, 2351, 2834, 3223
BtsI GCAGTG 2 cut(s) 1835, 2820
BtsIMutI CAGTG 6 cut(s) 357, 1835, 2152, 2188, 2451, 2820
Cac8I GCNNGC 9 cut(s) 1305, 1385, 1646, 1873, 2076, 2559, 2600, 3016, 3042
CaiI CAGNNNCTG 3 cut(s) 215, 608, 1715
CfoI GCGC 1 cut(s) 73
Cfr10I RCCGGY 2 cut(s) 2822, 3040
Cfr13I GGNCC 5 cut(s) 479, 621, 2348, 3032, 3457
ClaI ATCGAT 2 cut(s) 443, 915
CseI GACGC 1 cut(s) 169
CsiI ACCWGGT 1 cut(s) 1189
Csp6I GTAC 7 cut(s) 387, 743, 1289, 2292, 2424, 2828, 3236
CspAI ACCGGT 1 cut(s) 2822
CviQI GTAC 7 cut(s) 387, 743, 1289, 2292, 2424, 2828, 3236
DraI TTTAAA 1 cut(s) 2221
DraIII CACNNNGTG 1 cut(s) 1094
EaeI YGGCCR 1 cut(s) 3220
Eam1104I CTCTTC 7 cut(s) 645, 1368, 1455, 1542, 1956, 2308, 3066
EarI CTCTTC 7 cut(s) 645, 1368, 1455, 1542, 1956, 2308, 3066
EciI GGCGGA 2 cut(s) 94, 166
Eco130I CCWWGG 3 cut(s) 2016, 3460, 3571
Eco24I GRGCYC 1 cut(s) 1679
Eco31I GGTCTC 1 cut(s) 3068
Eco32I GATATC 1 cut(s) 3701
Eco47I GGWCC 4 cut(s) 479, 621, 2348, 3457
Eco57I CTGAAG 8 cut(s) 330, 555, 903, 1392, 2274, 3090, 3345, 3677
Eco88I CYCGRG 1 cut(s) 1119
EcoNI CCTNNNNNAGG 2 cut(s) 2783, 3110
EcoO109I RGGNCCY 1 cut(s) 621
EcoRI GAATTC 1 cut(s) 2440
EcoRII CCWGG 4 cut(s) 505, 1189, 2524, 3217
EcoRV GATATC 1 cut(s) 3701
EcoT14I CCWWGG 3 cut(s) 2016, 3460, 3571
EcoT22I ATGCAT 3 cut(s) 582, 950, 1400
EcoT38I GRGCYC 1 cut(s) 1679
ErhI CCWWGG 3 cut(s) 2016, 3460, 3571
Esp3I CGTCTC 3 cut(s) 152, 2704, 3627
FalI AAGNNNNNCTT 6 cut(s) 1628, 1660, 2268, 2300, 2748, 2780
FaqI GGGAC 3 cut(s) 158, 916, 2361
FauNDI CATATG 1 cut(s) 3046
FblI GTMKAC 1 cut(s) 2656
FokI GGATG 5 cut(s) 957, 2015, 2338, 2821, 3210
FriOI GRGCYC 1 cut(s) 1679
GlaI GCGC 1 cut(s) 72
GsuI CTGGAG 1 cut(s) 630
HaeIII GGCC 4 cut(s) 328, 1705, 3034, 3222
HapII CCGG 7 cut(s) 42, 112, 142, 1239, 2823, 3041, 3334
HgaI GACGC 1 cut(s) 169
HhaI GCGC 1 cut(s) 73
Hin1I GRCGYC 1 cut(s) 161
Hin6I GCGC 1 cut(s) 71
HinP1I GCGC 1 cut(s) 71
HindIII AAGCTT 2 cut(s) 1099, 1226
HpaI GTTAAC 1 cut(s) 708
HpaII CCGG 7 cut(s) 42, 112, 142, 1239, 2823, 3041, 3334
HphI GGTGA 9 cut(s) 212, 260, 296, 302, 729, 809, 900, 1179, 3158
Hpy99I CGWCG 1 cut(s) 797
HpyCH4IV ACGT 5 cut(s) 982, 1925, 2713, 2872, 3109
HpyF10VI GCNNNNNNNGC 6 cut(s) 85, 397, 1107, 2339, 2567, 3499
HpySE526I ACGT 5 cut(s) 982, 1925, 2713, 2872, 3109
Hsp92I GRCGYC 1 cut(s) 161
HspAI GCGC 1 cut(s) 71
Kpn2I TCCGGA 1 cut(s) 1238
KroI GCCGGC 1 cut(s) 3040
KroNI GCCGGC 1 cut(s) 3042
KspAI GTTAAC 1 cut(s) 708
LguI GCTCTTC 1 cut(s) 2308
LmnI GCTCC 3 cut(s) 2566, 3396, 3487
Lsp1109I GCAGC 8 cut(s) 857, 1880, 1978, 2173, 2377, 2760, 3049, 3502
LweI GCATC 6 cut(s) 14, 567, 935, 1940, 2464, 2637
MabI ACCWGGT 1 cut(s) 1189
MaeII ACGT 5 cut(s) 982, 1925, 2713, 2872, 3109
MaeIII GTNAC 8 cut(s) 456, 670, 983, 1072, 1185, 2011, 2681, 3556
MfeI CAATTG 4 cut(s) 395, 1266, 3297, 3678
MflI RGATCY 3 cut(s) 22, 680, 3101
MhlI GDGCHC 3 cut(s) 1126, 1679, 2822
MlsI TGGCCA 1 cut(s) 3222
MluNI TGGCCA 1 cut(s) 3222
MlyI GAGTC 7 cut(s) 598, 614, 767, 1111, 2972, 3323, 3711
MmeI TCCRAC 4 cut(s) 498, 1925, 3345, 3670
Mox20I TGGCCA 1 cut(s) 3222
Mph1103I ATGCAT 3 cut(s) 582, 950, 1400
MroI TCCGGA 1 cut(s) 1238
MroNI GCCGGC 1 cut(s) 3040
MroXI GAANNNNTTC 2 cut(s) 876, 2283
MscI TGGCCA 1 cut(s) 3222
MseI TTAA 9 cut(s) 501, 707, 2220, 2615, 2690, 2903, 3270, 3306, 3318
MslI CAYNNNNRTG 4 cut(s) 2460, 2640, 2691, 3059
Msp20I TGGCCA 1 cut(s) 3222
MspA1I CMGCKG 2 cut(s) 2252, 3485
MspCI CTTAAG 1 cut(s) 2689
MspI CCGG 7 cut(s) 42, 112, 142, 1239, 2823, 3041, 3334
MspR9I CCNGG 7 cut(s) 113, 143, 507, 1191, 2526, 3219, 3334
MunI CAATTG 4 cut(s) 395, 1266, 3297, 3678
Mva1269I GAATGC 1 cut(s) 948
MvaI CCWGG 4 cut(s) 507, 1191, 2526, 3219
MvnI CGCG 2 cut(s) 182, 264
MwoI GCNNNNNNNGC 6 cut(s) 85, 397, 1107, 2339, 2567, 3499
NaeI GCCGGC 1 cut(s) 3042
NciI CCSGG 3 cut(s) 113, 143, 3334
NcoI CCATGG 1 cut(s) 3571
NdeI CATATG 1 cut(s) 3046
NgoMIV GCCGGC 1 cut(s) 3040
NlaIV GGNNCC 5 cut(s) 367, 623, 2349, 2698, 3352
NmuCI GTSAC 4 cut(s) 1185, 2011, 2681, 3556
NsiI ATGCAT 3 cut(s) 582, 950, 1400
NspI RCATGY 4 cut(s) 1307, 1387, 2342, 3564
NspV TTCGAA 1 cut(s) 2438
OliI CACNNNNGTG 1 cut(s) 2691
PaeI GCATGC 2 cut(s) 1307, 1387
PciI ACATGT 1 cut(s) 3560
PciSI GCTCTTC 1 cut(s) 2308
PcsI WCGNNNNNNNCGW 2 cut(s) 153, 572
PctI GAATGC 1 cut(s) 948
PdiI GCCGGC 1 cut(s) 3042
PdmI GAANNNNTTC 2 cut(s) 876, 2283
PflFI GACNNNGTC 1 cut(s) 3720
PflMI CCANNNNNTGG 1 cut(s) 1268
PfoI TCCNGGA 3 cut(s) 505, 2524, 3332
PinAI ACCGGT 1 cut(s) 2822
PleI GAGTC 7 cut(s) 597, 613, 767, 1111, 2972, 3323, 3711
PpsI GAGTC 7 cut(s) 597, 613, 767, 1111, 2972, 3323, 3711
PpuMI RGGWCCY 1 cut(s) 621
PscI ACATGT 1 cut(s) 3560
PshBI ATTAAT 1 cut(s) 501
PsiI TTATAA 1 cut(s) 2933
Psp1406I AACGTT 1 cut(s) 982
Psp5II RGGWCCY 1 cut(s) 621
Psp6I CCWGG 4 cut(s) 505, 1189, 2524, 3217
PspGI CCWGG 4 cut(s) 505, 1189, 2524, 3217
PspN4I GGNNCC 5 cut(s) 367, 623, 2349, 2698, 3352
PspPI GGNCC 5 cut(s) 479, 621, 2348, 3032, 3457
PspPPI RGGWCCY 1 cut(s) 621
PstI CTGCAG 1 cut(s) 966
PstNI CAGNNNCTG 3 cut(s) 215, 608, 1715
PsuI RGATCY 3 cut(s) 22, 680, 3101
PsyI GACNNNGTC 1 cut(s) 3720
PvuII CAGCTG 1 cut(s) 2252
RsaI GTAC 7 cut(s) 388, 744, 1290, 2293, 2425, 2829, 3237
RsaNI GTAC 7 cut(s) 387, 743, 1289, 2292, 2424, 2828, 3236
RseI CAYNNNNRTG 4 cut(s) 2460, 2640, 2691, 3059
SapI GCTCTTC 1 cut(s) 2308
SaqAI TTAA 9 cut(s) 501, 707, 2220, 2615, 2690, 2903, 3270, 3306, 3318
Sau96I GGNCC 5 cut(s) 479, 621, 2348, 3032, 3457
SchI GAGTC 7 cut(s) 598, 614, 767, 1111, 2972, 3323, 3711
ScrFI CCNGG 7 cut(s) 113, 143, 507, 1191, 2526, 3219, 3334
SduI GDGCHC 3 cut(s) 1126, 1679, 2822
SexAI ACCWGGT 1 cut(s) 1189
SfaNI GCATC 6 cut(s) 14, 567, 935, 1940, 2464, 2637
SfcI CTRYAG 4 cut(s) 66, 962, 2268, 3181
SfuI TTCGAA 1 cut(s) 2438
SgrAI CRCCGGYG 1 cut(s) 2822
SinI GGWCC 4 cut(s) 479, 621, 2348, 3457
SmiMI CAYNNNNRTG 4 cut(s) 2460, 2640, 2691, 3059
SmlI CTYRAG 6 cut(s) 322, 2584, 2689, 2765, 2780, 3529
SmoI CTYRAG 6 cut(s) 322, 2584, 2689, 2765, 2780, 3529
SpeI ACTAGT 2 cut(s) 1126, 3238
SphI GCATGC 2 cut(s) 1307, 1387
SspI AATATT 1 cut(s) 335
StyD4I CCNGG 7 cut(s) 111, 141, 505, 1189, 2524, 3217, 3332
StyI CCWWGG 3 cut(s) 2016, 3460, 3571
TaiI ACGT 5 cut(s) 985, 1928, 2716, 2875, 3112
TaqI TCGA 9 cut(s) 443, 575, 776, 915, 1983, 2415, 2438, 3552, 3647
TaqII GACCGA 1 cut(s) 467
TatI WGTACW 4 cut(s) 1288, 2423, 2827, 3235
TauI GCSGC 5 cut(s) 88, 166, 182, 2490, 3037
Tru1I TTAA 9 cut(s) 501, 707, 2220, 2615, 2690, 2903, 3270, 3306, 3318
Tru9I TTAA 9 cut(s) 501, 707, 2220, 2615, 2690, 2903, 3270, 3306, 3318
TscAI CASTG 6 cut(s) 364, 1842, 2152, 2188, 2451, 2820
TseFI GTSAC 4 cut(s) 1185, 2011, 2681, 3556
TseI GCWGC 8 cut(s) 845, 1868, 1966, 2186, 2390, 2773, 3037, 3490
Tsp45I GTSAC 4 cut(s) 1185, 2011, 2681, 3556
TspGWI ACGGA 5 cut(s) 115, 291, 1807, 2876, 3599
TspRI CASTG 6 cut(s) 364, 1842, 2152, 2188, 2451, 2820
Tth111I GACNNNGTC 1 cut(s) 3720
Van91I CCANNNNNTGG 1 cut(s) 1268
Vha464I CTTAAG 1 cut(s) 2689
VpaK11BI GGWCC 4 cut(s) 479, 621, 2348, 3457
VspI ATTAAT 1 cut(s) 501
XagI CCTNNNNNAGG 2 cut(s) 2783, 3110
XceI RCATGY 4 cut(s) 1307, 1387, 2342, 3564
XcmI CCANNNNNNNNNTGG 2 cut(s) 1324, 3361
XmiI GTMKAC 1 cut(s) 2656
XmnI GAANNNNTTC 2 cut(s) 876, 2283
Zsp2I ATGCAT 3 cut(s) 582, 950, 1400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.