MD09G1132400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005008
Physical Location & Seq
Reverse (-)
10171942 .. 10172817
876 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1132400.v1.1.491

Sequence Viewer

Length: 735 bp
ATGTCTTTAACAAAACCGAAGATGGTTACCCTTCAGTCTTCTGTCCCTGCTGATAAGGTCAGAAGTGGGGAAGATTCAAATGACATGAGTATCATAGAAGAAAATCCACATAAGTATGATTATGGTAGATTATCTCCAATATTAGATGCACTTCTTGCAGCTGGCAGCAAGGATATGCATGCTGACTCAGTTGCCGTCCCTGCCACTTCAATGGCACCGAACGGGGGTGAGGTATCTGCATCAGATGAAAATGGGATTGGCCACATGAGAAATGGTAGAACTACTGAAATGGGCAATTTCGGCACCAATGACTTGTCTACTGAGGTAGAATCTGTTTCTCGTATCAAGTTGAGTGAGTCAAATGGTGGTTATCTTCATCAGACTGCTTACGATGCCTCATCTAACGGAAATCATAATTCAGCTGCTAGTGATTCTGGTGATGGCCAAATCCAGACCCAGAATCAATTAAACAATGTAAGAATGTCTCTTGCAATCAAGATTTTGATAGAACTGTTGCCGGTGTGGTTTGATCTCATACATTCGGTATTTTACATTAGTAGATTTGATGATGGACGTTTTAGTTGTTGTTCTGAGGGTGGTTGCATTGTTACACTGAAATTTTCAATGGTTGGGTTCTTTAATAAAATGGGATGTGAGAATGAAGGTAATGACAGTGGAAAATTAGGAACTTGGATATTTGGATATTGGATTGTACAACAGAAGCTGAAATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

245

Amino Acids

26.54

Weight (kDa)

5.1

Isoelectric Point (pI)

46.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 214, 302
AccI GTMKAC 1 cut(s) 317
AcoI YGGCCR 2 cut(s) 259, 442
AcsI RAATTY 2 cut(s) 617, 728
AcuI CTGAAG 1 cut(s) 17
AfaI GTAC 1 cut(s) 714
AfiI CCNNNNNNNGG 1 cut(s) 224
AgsI TTSAA 3 cut(s) 78, 210, 624
AjuI GAANNNNNNNTTGG 2 cut(s) 240, 272
AluBI AGCT 3 cut(s) 161, 422, 724
AluI AGCT 3 cut(s) 161, 422, 724
Alw26I GTCTC 1 cut(s) 489
AlwNI CAGNNNCTG 1 cut(s) 724
AoxI GGCC 2 cut(s) 259, 442
ApeKI GCWGC 3 cut(s) 158, 165, 422
ApoI RAATTY 2 cut(s) 617, 728
AsuHPI GGTGA 2 cut(s) 239, 449
BaeI ACNNNNGTAYC 2 cut(s) 73, 106
BalI TGGCCA 2 cut(s) 261, 444
BanI GGYRCC 2 cut(s) 214, 302
BbsI GAAGAC 1 cut(s) 30
BbvI GCAGC 3 cut(s) 170, 177, 409
BccI CCATC 3 cut(s) 16, 434, 563
BceAI ACGGC 1 cut(s) 179
BcoDI GTCTC 1 cut(s) 489
BfaI CTAG 1 cut(s) 426
BisI GCNGC 3 cut(s) 159, 166, 423
BlsI GCNGC 3 cut(s) 160, 167, 424
BmiI GGNNCC 2 cut(s) 216, 304
BmsI GCATC 3 cut(s) 136, 248, 382
BpiI GAAGAC 1 cut(s) 30
Bsc4I CCNNNNNNNGG 1 cut(s) 224
Bse118I RCCGGY 1 cut(s) 517
BseGI GGATG 1 cut(s) 656
BseLI CCNNNNNNNGG 1 cut(s) 224
BseMII CTCAG 3 cut(s) 201, 312, 582
BseXI GCAGC 3 cut(s) 170, 177, 409
BshFI GGCC 2 cut(s) 261, 444
BshNI GGYRCC 2 cut(s) 214, 302
BsiSI CCGG 1 cut(s) 518
BslFI GGGAC 2 cut(s) 29, 182
BslI CCNNNNNNNGG 1 cut(s) 224
BsmAI GTCTC 1 cut(s) 489
BsmFI GGGAC 2 cut(s) 29, 182
BsnI GGCC 2 cut(s) 261, 444
Bsp1407I TGTACA 1 cut(s) 712
Bsp143I GATC 1 cut(s) 529
BspANI GGCC 2 cut(s) 261, 444
BspCNI CTCAG 3 cut(s) 200, 313, 583
BspLI GGNNCC 2 cut(s) 216, 304
BspT107I GGYRCC 2 cut(s) 214, 302
BsrFI RCCGGY 1 cut(s) 517
BsrGI TGTACA 1 cut(s) 712
BssAI RCCGGY 1 cut(s) 517
BssMI GATC 1 cut(s) 529
Bst4CI ACNGT 2 cut(s) 513, 674
BstAPI GCANNNNNTGC 1 cut(s) 155
BstAUI TGTACA 1 cut(s) 712
BstC8I GCNNGC 2 cut(s) 163, 180
BstDEI CTNAG 3 cut(s) 187, 321, 591
BstEII GGTNACC 1 cut(s) 25
BstF5I GGATG 1 cut(s) 656
BstKTI GATC 1 cut(s) 532
BstMAI GTCTC 1 cut(s) 489
BstMBI GATC 1 cut(s) 529
BstMWI GCNNNNNNNGC 4 cut(s) 155, 200, 300, 392
BstNSI RCATGY 1 cut(s) 182
BstPI GGTNACC 1 cut(s) 25
BstV1I GCAGC 3 cut(s) 170, 177, 409
BstV2I GAAGAC 1 cut(s) 30
BstXI CCANNNNNNTGG 1 cut(s) 211
BsuRI GGCC 2 cut(s) 261, 444
BtsCI GGATG 1 cut(s) 656
BtsIMutI CAGTG 2 cut(s) 611, 679
Cac8I GCNNGC 2 cut(s) 163, 180
CaiI CAGNNNCTG 1 cut(s) 724
Cfr10I RCCGGY 1 cut(s) 517
Csp6I GTAC 1 cut(s) 713
CviAII CATG 3 cut(s) 85, 179, 265
CviJI RGCY 5 cut(s) 161, 261, 422, 444, 724
CviKI_1 RGCY 5 cut(s) 161, 261, 422, 444, 724
CviQI GTAC 1 cut(s) 713
DdeI CTNAG 3 cut(s) 187, 321, 591
DpnI GATC 1 cut(s) 531
DpnII GATC 1 cut(s) 529
EaeI YGGCCR 2 cut(s) 259, 442
Eco57I CTGAAG 1 cut(s) 17
Eco91I GGTNACC 1 cut(s) 25
EcoO65I GGTNACC 1 cut(s) 25
EcoT22I ATGCAT 1 cut(s) 180
FaeI CATG 3 cut(s) 88, 182, 268
FaqI GGGAC 2 cut(s) 29, 182
FatI CATG 3 cut(s) 84, 178, 264
FblI GTMKAC 1 cut(s) 317
Fnu4HI GCNGC 3 cut(s) 159, 166, 423
FokI GGATG 1 cut(s) 663
Fsp4HI GCNGC 3 cut(s) 159, 166, 423
FspBI CTAG 1 cut(s) 426
GluI GCNGC 3 cut(s) 159, 166, 423
HaeIII GGCC 2 cut(s) 261, 444
HapII CCGG 1 cut(s) 518
Hin1II CATG 3 cut(s) 88, 182, 268
HinfI GANTC 6 cut(s) 74, 185, 329, 356, 431, 460
HpaII CCGG 1 cut(s) 518
HphI GGTGA 2 cut(s) 239, 449
Hpy166II GTNNAC 1 cut(s) 318
Hpy188I TCNGA 4 cut(s) 62, 244, 381, 592
Hpy188III TCNNGA 2 cut(s) 451, 496
Hpy8I GTNNAC 1 cut(s) 318
HpyAV CCTTC 2 cut(s) 41, 656
HpyCH4III ACNGT 2 cut(s) 513, 674
HpyCH4IV ACGT 1 cut(s) 574
HpyCH4V TGCA 6 cut(s) 149, 158, 178, 239, 491, 603
HpyF10VI GCNNNNNNNGC 4 cut(s) 155, 200, 300, 392
HpyF3I CTNAG 3 cut(s) 187, 321, 591
HpySE526I ACGT 1 cut(s) 574
Hsp92II CATG 3 cut(s) 88, 182, 268
Kzo9I GATC 1 cut(s) 529
LpnPI CCDG 7 cut(s) 60, 147, 213, 420, 464, 470, 531
Lsp1109I GCAGC 3 cut(s) 170, 177, 409
LweI GCATC 3 cut(s) 136, 248, 382
MaeI CTAG 1 cut(s) 426
MaeII ACGT 1 cut(s) 574
MaeIII GTNAC 2 cut(s) 25, 607
MalI GATC 1 cut(s) 531
MboI GATC 1 cut(s) 529
MboII GAAGA 5 cut(s) 30, 31, 83, 110, 365
MlsI TGGCCA 2 cut(s) 261, 444
MluCI AATT 6 cut(s) 295, 415, 464, 617, 680, 728
MluNI TGGCCA 2 cut(s) 261, 444
MlyI GAGTC 2 cut(s) 179, 365
MnlI CCTC 4 cut(s) 223, 316, 406, 586
Mox20I TGGCCA 2 cut(s) 261, 444
Mph1103I ATGCAT 1 cut(s) 180
MscI TGGCCA 2 cut(s) 261, 444
MseI TTAA 3 cut(s) 8, 467, 639
MslI CAYNNNNRTG 2 cut(s) 114, 209
Msp20I TGGCCA 2 cut(s) 261, 444
MspA1I CMGCKG 2 cut(s) 161, 422
MspI CCGG 1 cut(s) 518
MwoI GCNNNNNNNGC 4 cut(s) 155, 200, 300, 392
NdeII GATC 1 cut(s) 529
NlaIII CATG 3 cut(s) 88, 182, 268
NlaIV GGNNCC 2 cut(s) 216, 304
NsiI ATGCAT 1 cut(s) 180
NspI RCATGY 1 cut(s) 182
PaeI GCATGC 1 cut(s) 182
PfeI GAWTC 4 cut(s) 74, 329, 431, 460
PkrI GCNGC 3 cut(s) 160, 167, 424
PleI GAGTC 2 cut(s) 179, 364
PpsI GAGTC 2 cut(s) 179, 364
PspEI GGTNACC 1 cut(s) 25
PspN4I GGNNCC 2 cut(s) 216, 304
PstNI CAGNNNCTG 1 cut(s) 724
PvuII CAGCTG 2 cut(s) 161, 422
RsaI GTAC 1 cut(s) 714
RsaNI GTAC 1 cut(s) 713
RseI CAYNNNNRTG 2 cut(s) 114, 209
SaqAI TTAA 3 cut(s) 8, 467, 639
SatI GCNGC 3 cut(s) 159, 166, 423
Sau3AI GATC 1 cut(s) 529
SchI GAGTC 2 cut(s) 179, 365
SetI ASST 8 cut(s) 60, 163, 234, 327, 424, 577, 667, 726
SfaNI GCATC 3 cut(s) 136, 248, 382
SmiMI CAYNNNNRTG 2 cut(s) 114, 209
SphI GCATGC 1 cut(s) 182
Sse9I AATT 6 cut(s) 295, 415, 464, 617, 680, 728
SspI AATATT 1 cut(s) 141
SspMI CTAG 1 cut(s) 426
TaaI ACNGT 2 cut(s) 513, 674
TaiI ACGT 1 cut(s) 577
TasI AATT 6 cut(s) 295, 415, 464, 617, 680, 728
TatI WGTACW 1 cut(s) 712
TfiI GAWTC 4 cut(s) 74, 329, 431, 460
Tru1I TTAA 3 cut(s) 8, 467, 639
Tru9I TTAA 3 cut(s) 8, 467, 639
TscAI CASTG 2 cut(s) 618, 679
TseI GCWGC 3 cut(s) 158, 165, 422
TspDTI ATGAA 3 cut(s) 261, 365, 675
TspGWI ACGGA 1 cut(s) 420
TspRI CASTG 2 cut(s) 618, 679
XapI RAATTY 2 cut(s) 617, 728
XceI RCATGY 1 cut(s) 182
XcmI CCANNNNNNNNNTGG 1 cut(s) 269
XmiI GTMKAC 1 cut(s) 317
XspI CTAG 1 cut(s) 426
Zsp2I ATGCAT 1 cut(s) 180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.