Rorug02G0447800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0005008
Physical Location & Seq
Reverse (-)
57431151 .. 57432794
1644 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0447800.1

Sequence Viewer

Length: 1185 bp
ATGTCAGGATTTGCTGCTGATTTTGTTCCTTATGTTCACATCAACAACATGACTCACAAGTCAACACGTAAAACAGAACATATCCGAATGGCAACTTTCAAGTTTCCTATTTACATCCTGGCTTTATGTGCAGGCTTTCTTATTCCAAGCAGCTGTCATGATGGTTATACTGGGATTCAGAAAAAACATGCAGCCTTGTTCATCTTTGGTGATTCAGTATTTGATGTTGGAAACAATAACTACATAAATACAACCACTACTTTTCAGTCAAATTTCTGGCCCTATGGGGAATCTTATTTCAGATACCCAACTGGCAGATTTTCTGATGGGCGTCTAATCCCCGATTTTATAGCTGAGTATGCAAATTTACCTTTTATACAACCATACTTACAACCCGGGTTCGATGACTATACTTATGGGGTGAACTTTGCTTCTGGTGGGGGTGGTGCTCTAGCTGAAACATATCAAGCATTTGCCATAGGCTTAAACACTCAATTGAGTTATTTCAAGAATGTGGAGAAGCAATTGAGGCACAAACTAGGTGATGGAGAAGCCTACATATTTCTTTCTAAAGCTGTTTACCTAATTAGCATTGGAGCCAATGATTATTACACCCCACTTATGAAAAATTACAGCTCGACTCAAGATGAAGAATATGTTGGCATGGTGATTGGCAACCTCACAGTTGTGATCAGAGAAATATATAAGGTAGGTGGAAGGAAATTCGGGTTTGCAAACGTGTTACCTCTGGGTTGTTTACCAAGCTTTAGAATAAAGAATCCAGAAAACATTGGAGCCTGTATGAAAGAAGCTATGTCACTGGTGAAATCTCACAATAAAGAACTTGCTAAAGTGCTCCTAAAGTTGAAGAGCCAGCTCCAAGGATTCAAATATTCCAATGCCGATTTCTACACATACCTAAGTGAAAGAATGAATAACCCATCAAAACACGGTTTCAGGGAAGGAAAAGCGGCATGTTGTGGCTCGGGTCCATACAGAGGACATCAGAACTGTGGAGGGAAGGGAGGTGTGAAAGAGTATGAATTATGTGACAATGTTACCGAATATGTTTTTTTTGACAATCATCCAACTGAAAGGGTTTACCATCAAATTTCCAAGATATGGTGGAACCAAACTCCAAATGTCACAGGGATTTACAATCTGAGATCTCTATTTGAAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

394

Amino Acids

44.5

Weight (kDa)

8.68

Isoelectric Point (pI)

34.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 66 - 373 6.6e-28 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 58
AccB7I CCANNNNNTGG 1 cut(s) 1122
AciI CCGC 1 cut(s) 971
AcsI RAATTY 4 cut(s) 271, 364, 722, 1110
AcyI GRCGYC 1 cut(s) 331
AfiI CCNNNNNNNGG 2 cut(s) 998, 1122
AflIII ACRYGT 2 cut(s) 65, 738
AgsI TTSAA 5 cut(s) 100, 508, 868, 889, 1178
AjnI CCWGG 1 cut(s) 117
AjuI GAANNNNNNNTTGG 4 cut(s) 642, 674, 890, 922
AleI CACNNNNGTG 1 cut(s) 686
AluBI AGCT 8 cut(s) 153, 353, 455, 575, 636, 765, 812, 877
AluI AGCT 8 cut(s) 153, 353, 455, 575, 636, 765, 812, 877
Alw21I GWGCWC 2 cut(s) 451, 858
Ama87I CYCGRG 2 cut(s) 395, 985
AoxI GGCC 1 cut(s) 278
ApeKI GCWGC 3 cut(s) 14, 150, 191
ApoI RAATTY 4 cut(s) 271, 364, 722, 1110
AspS9I GGNCC 2 cut(s) 279, 989
AsuC2I CCSGG 2 cut(s) 396, 397
AsuHPI GGTGA 5 cut(s) 221, 433, 554, 679, 835
AvaI CYCGRG 2 cut(s) 395, 985
AvaII GGWCC 1 cut(s) 989
Bbv12I GWGCWC 2 cut(s) 451, 858
BbvI GCAGC 2 cut(s) 162, 203
BccI CCATC 5 cut(s) 155, 320, 539, 949, 1113
BciT130I CCWGG 1 cut(s) 119
BclI TGATCA 1 cut(s) 690
BcnI CCSGG 2 cut(s) 396, 397
BfaI CTAG 2 cut(s) 452, 539
BglII AGATCT 1 cut(s) 1166
BisI GCNGC 4 cut(s) 15, 151, 192, 972
BlsI GCNGC 4 cut(s) 16, 152, 193, 973
Bme1390I CCNGG 3 cut(s) 119, 396, 397
Bme18I GGWCC 1 cut(s) 989
BmeT110I CYCGRG 2 cut(s) 395, 985
BmgT120I GGNCC 2 cut(s) 279, 989
BmiI GGNNCC 4 cut(s) 598, 796, 990, 1130
BmrFI CCNGG 3 cut(s) 119, 396, 397
BmrI ACTGGG 1 cut(s) 180
BmuI ACTGGG 1 cut(s) 180
BpuEI CTTGAG 1 cut(s) 627
BpuMI CCSGG 2 cut(s) 396, 397
BsaAI YACGTR 1 cut(s) 68
BsaHI GRCGYC 1 cut(s) 331
BsaJI CCNNGG 2 cut(s) 395, 880
Bsc4I CCNNNNNNNGG 2 cut(s) 998, 1122
Bse1I ACTGG 3 cut(s) 175, 316, 825
BseBI CCWGG 1 cut(s) 119
BseDI CCNNGG 2 cut(s) 395, 880
BseGI GGATG 2 cut(s) 114, 1084
BseLI CCNNNNNNNGG 2 cut(s) 998, 1122
BseMII CTCAG 2 cut(s) 345, 1154
BseNI ACTGG 3 cut(s) 175, 316, 825
BseXI GCAGC 2 cut(s) 162, 203
BsgI GTGCAG 1 cut(s) 150
BshFI GGCC 1 cut(s) 280
BsiHKAI GWGCWC 2 cut(s) 451, 858
BsiHKCI CYCGRG 2 cut(s) 395, 985
BsiSI CCGG 1 cut(s) 396
BslI CCNNNNNNNGG 2 cut(s) 998, 1122
BsnI GGCC 1 cut(s) 280
BsoBI CYCGRG 2 cut(s) 395, 985
Bsp1286I GDGCHC 2 cut(s) 451, 858
Bsp143I GATC 2 cut(s) 690, 1166
BspACI CCGC 1 cut(s) 971
BspANI GGCC 1 cut(s) 280
BspCNI CTCAG 2 cut(s) 346, 1155
BspHI TCATGA 1 cut(s) 157
BspLI GGNNCC 4 cut(s) 598, 796, 990, 1130
BspQI GCTCTTC 1 cut(s) 863
BsrI ACTGG 3 cut(s) 175, 316, 825
BssECI CCNNGG 2 cut(s) 395, 880
BssMI GATC 2 cut(s) 690, 1166
BssNI GRCGYC 1 cut(s) 331
BssT1I CCWWGG 1 cut(s) 880
Bst2UI CCWGG 1 cut(s) 119
Bst4CI ACNGT 3 cut(s) 685, 953, 1013
Bst6I CTCTTC 1 cut(s) 863
BstACI GRCGYC 1 cut(s) 331
BstBAI YACGTR 1 cut(s) 68
BstC8I GCNNGC 2 cut(s) 133, 875
BstDEI CTNAG 3 cut(s) 354, 920, 1163
BstF5I GGATG 2 cut(s) 114, 1084
BstKTI GATC 2 cut(s) 693, 1169
BstMBI GATC 2 cut(s) 690, 1166
BstMWI GCNNNNNNNGC 3 cut(s) 128, 359, 529
BstNI CCWGG 1 cut(s) 119
BstNSI RCATGY 2 cut(s) 191, 978
BstSCI CCNGG 3 cut(s) 117, 394, 395
BstV1I GCAGC 2 cut(s) 162, 203
BstX2I RGATCY 1 cut(s) 1166
BstYI RGATCY 1 cut(s) 1166
BsuRI GGCC 1 cut(s) 280
BtsCI GGATG 2 cut(s) 114, 1084
BtsIMutI CAGTG 1 cut(s) 818
Cac8I GCNNGC 2 cut(s) 133, 875
CciI TCATGA 1 cut(s) 157
Cfr13I GGNCC 2 cut(s) 279, 989
Cfr9I CCCGGG 1 cut(s) 395
CseI GACGC 1 cut(s) 320
CviAII CATG 5 cut(s) 49, 158, 188, 664, 975
DdeI CTNAG 3 cut(s) 354, 920, 1163
DpnI GATC 2 cut(s) 692, 1168
DpnII GATC 2 cut(s) 690, 1166
DrdI GACNNNNNNGTC 1 cut(s) 58
DseDI GACNNNNNNGTC 1 cut(s) 58
Eam1104I CTCTTC 1 cut(s) 863
EarI CTCTTC 1 cut(s) 863
Eco130I CCWWGG 1 cut(s) 880
Eco47I GGWCC 1 cut(s) 989
Eco88I CYCGRG 2 cut(s) 395, 985
EcoRII CCWGG 1 cut(s) 117
EcoT14I CCWWGG 1 cut(s) 880
ErhI CCWWGG 1 cut(s) 880
FaeI CATG 5 cut(s) 52, 161, 191, 667, 978
FatI CATG 5 cut(s) 48, 157, 187, 663, 974
FbaI TGATCA 1 cut(s) 690
Fnu4HI GCNGC 4 cut(s) 15, 151, 192, 972
FokI GGATG 2 cut(s) 101, 1071
Fsp4HI GCNGC 4 cut(s) 15, 151, 192, 972
FspBI CTAG 2 cut(s) 452, 539
GluI GCNGC 4 cut(s) 15, 151, 192, 972
HaeIII GGCC 1 cut(s) 280
HapII CCGG 1 cut(s) 396
HgaI GACGC 1 cut(s) 320
Hin1I GRCGYC 1 cut(s) 331
Hin1II CATG 5 cut(s) 52, 161, 191, 667, 978
HincII GTYRAC 1 cut(s) 63
HindII GTYRAC 1 cut(s) 63
HindIII AAGCTT 1 cut(s) 763
HinfI GANTC 7 cut(s) 52, 175, 212, 290, 640, 778, 885
HpaII CCGG 1 cut(s) 396
HphI GGTGA 5 cut(s) 221, 433, 554, 679, 835
Hpy166II GTNNAC 6 cut(s) 37, 63, 424, 580, 758, 1102
Hpy188I TCNGA 7 cut(s) 86, 180, 302, 325, 695, 1008, 1164
Hpy188III TCNNGA 5 cut(s) 6, 158, 508, 644, 782
Hpy8I GTNNAC 6 cut(s) 37, 63, 424, 580, 758, 1102
HpyAV CCTTC 3 cut(s) 711, 956, 1015
HpyCH4III ACNGT 3 cut(s) 685, 953, 1013
HpyCH4IV ACGT 2 cut(s) 67, 738
HpyCH4V TGCA 4 cut(s) 131, 191, 362, 734
HpyF10VI GCNNNNNNNGC 3 cut(s) 128, 359, 529
HpyF3I CTNAG 3 cut(s) 354, 920, 1163
HpySE526I ACGT 2 cut(s) 67, 738
Hsp92I GRCGYC 1 cut(s) 331
Hsp92II CATG 5 cut(s) 52, 161, 191, 667, 978
Ksp22I TGATCA 1 cut(s) 690
Kzo9I GATC 2 cut(s) 690, 1166
LguI GCTCTTC 1 cut(s) 863
LmnI GCTCC 4 cut(s) 596, 794, 861, 882
Lsp1109I GCAGC 2 cut(s) 162, 203
MaeI CTAG 2 cut(s) 452, 539
MaeII ACGT 2 cut(s) 67, 738
MaeIII GTNAC 5 cut(s) 741, 816, 1049, 1057, 1144
MalI GATC 2 cut(s) 692, 1168
MboI GATC 2 cut(s) 690, 1166
MboII GAAGA 2 cut(s) 662, 880
MfeI CAATTG 2 cut(s) 494, 524
MflI RGATCY 1 cut(s) 1166
MhlI GDGCHC 2 cut(s) 451, 858
MluCI AATT 9 cut(s) 271, 364, 494, 524, 585, 628, 722, 1043, 1110
MlyI GAGTC 2 cut(s) 46, 634
MmeI TCCRAC 2 cut(s) 208, 1112
MnlI CCTC 6 cut(s) 522, 689, 756, 992, 1010, 1019
MseI TTAA 1 cut(s) 485
MslI CAYNNNNRTG 1 cut(s) 686
MspA1I CMGCKG 1 cut(s) 153
MspI CCGG 1 cut(s) 396
MspR9I CCNGG 3 cut(s) 119, 396, 397
MunI CAATTG 2 cut(s) 494, 524
MvaI CCWGG 1 cut(s) 119
MwoI GCNNNNNNNGC 3 cut(s) 128, 359, 529
NciI CCSGG 2 cut(s) 396, 397
NdeII GATC 2 cut(s) 690, 1166
NlaIII CATG 5 cut(s) 52, 161, 191, 667, 978
NlaIV GGNNCC 4 cut(s) 598, 796, 990, 1130
NmuCI GTSAC 3 cut(s) 816, 1049, 1144
NspI RCATGY 2 cut(s) 191, 978
OliI CACNNNNGTG 1 cut(s) 686
PagI TCATGA 1 cut(s) 157
PciSI GCTCTTC 1 cut(s) 863
PfeI GAWTC 5 cut(s) 175, 212, 290, 778, 885
PflMI CCANNNNNTGG 1 cut(s) 1122
PkrI GCNGC 4 cut(s) 16, 152, 193, 973
PleI GAGTC 2 cut(s) 46, 634
PpsI GAGTC 2 cut(s) 46, 634
Ppu21I YACGTR 1 cut(s) 68
Psp6I CCWGG 1 cut(s) 117
PspGI CCWGG 1 cut(s) 117
PspN4I GGNNCC 4 cut(s) 598, 796, 990, 1130
PspPI GGNCC 2 cut(s) 279, 989
PsuI RGATCY 1 cut(s) 1166
PvuII CAGCTG 1 cut(s) 153
RseI CAYNNNNRTG 1 cut(s) 686
SapI GCTCTTC 1 cut(s) 863
SaqAI TTAA 1 cut(s) 485
SatI GCNGC 4 cut(s) 15, 151, 192, 972
Sau3AI GATC 2 cut(s) 690, 1166
Sau96I GGNCC 2 cut(s) 279, 989
SchI GAGTC 2 cut(s) 46, 634
ScrFI CCNGG 3 cut(s) 119, 396, 397
SduI GDGCHC 2 cut(s) 451, 858
SinI GGWCC 1 cut(s) 989
SmaI CCCGGG 1 cut(s) 397
SmiMI CAYNNNNRTG 1 cut(s) 686
SmlI CTYRAG 1 cut(s) 642
SmoI CTYRAG 1 cut(s) 642
Sse9I AATT 9 cut(s) 271, 364, 494, 524, 585, 628, 722, 1043, 1110
SsiI CCGC 1 cut(s) 971
SspI AATATT 1 cut(s) 893
SspMI CTAG 2 cut(s) 452, 539
StyD4I CCNGG 3 cut(s) 117, 394, 395
StyI CCWWGG 1 cut(s) 880
TaaI ACNGT 3 cut(s) 685, 953, 1013
TaiI ACGT 2 cut(s) 70, 741
TaqI TCGA 2 cut(s) 402, 638
TasI AATT 9 cut(s) 271, 364, 494, 524, 585, 628, 722, 1043, 1110
TauI GCSGC 1 cut(s) 974
TfiI GAWTC 5 cut(s) 175, 212, 290, 778, 885
Tru1I TTAA 1 cut(s) 485
Tru9I TTAA 1 cut(s) 485
TscAI CASTG 1 cut(s) 825
TseFI GTSAC 3 cut(s) 816, 1049, 1144
TseI GCWGC 3 cut(s) 14, 150, 191
Tsp45I GTSAC 3 cut(s) 816, 1049, 1144
TspDTI ATGAA 6 cut(s) 190, 638, 663, 818, 947, 1056
TspMI CCCGGG 1 cut(s) 395
TspRI CASTG 1 cut(s) 825
Van91I CCANNNNNTGG 1 cut(s) 1122
VpaK11BI GGWCC 1 cut(s) 989
XapI RAATTY 4 cut(s) 271, 364, 722, 1110
XceI RCATGY 2 cut(s) 191, 978
XmaI CCCGGG 1 cut(s) 395
XspI CTAG 2 cut(s) 452, 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.